hspa5b cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	hspa5b
Entrez Gene ID	100337562
Full Name	heat shock 70 kDa protein 5b
Synonyms	BiP/Grp78
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001172177.1	NP_001165648.1	heat shock 70 kDa protein 5b precursor

Pathways from BioSystems
Gene Ontology

The following hspa5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hspa5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf08511	NM_001172177.1 Latest version!	Xenopus laevis heat shock 70 kDa protein 5b (hspa5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hspa5b ( NM_001172177.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001165648.1 Xenopus laevis heat shock 70 kDa protein 5b (hspa5b), mRNA.

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CloneID	OAf08511
Clone ID Related Accession (Same CDS sequence)	NM_001172177.1
Accession Version	NM_001172177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-16
Organism	Xenopus laevis(African clawed frog)
Product	heat shock 70 kDa protein 5b precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U55069.1. ##Evidence-Data-START## Transcript exon combination :: U55069.1 [ECO:0000332] ##Evidence-Data-END##

ATGGTGACCA TGAAGCTGTT TGCCTTGGTG TTGATGGTGT CTGCCAGCGT ATTTGCCTCT 
GATGATGATG ACAAAAAAGA TGACATTGGG ACAGTGGTTG GAATAGATTT GGGCACAACA 
TACTCCTGTG TTGGAGTGTT TAAAAACGGA CGTGTAGAAA TTATTGCCAA TGACCAGGGT 
AACCGTATCA CACCCTCGTA TGTGGCATTT ACCCCAGAAG GTGAGCGTCT CATTGGAGAT 
GCTGCAAAAA ACCAGCTTAC ATCTAATCCA GAGAACACCG TGTTTGATGC CAAACGTCGT 
ATTGGCCGCA CATGGAATGA CCCTTCTGTT CAGCAGGACA TCAAGTACCT GCCATTTAAG 
GTAATTGAGA AGAAAACTAA ACCTTACATT GTAGTCGATG TTGGAGATCA GATGAAAACT 
TTTGCCCCAG AAGAGATTTC TGCTATGGTG CTAGTGAAGA TGAAGGAAAC TGCAGAGACT 
TACTTGGGCA GAAAGGTTAC TCATGCTGTT GTCACTGTAC CTGCTTATTT CAATGATGCA 
CAGCGTCAAG CCACCAAGGA TGCAGGAACT ATTGCAGGGC TGAATGTAAT GAGGATCATC 
AATGAACCCA CTGCTGCGGC CATTGCTTAT GGTCTGGACA AGAAAGAAGG AGAGAAGAAC 
ATCCTTGTGT TTGACTTGGG TGGTGGTACT TTTGATGTTT CCCTACTTAC CATCGATAAT 
GGTGTCTTTG AAGTGGTGGC TACTAATGGA GATACCCATC TTGGTGGAGA AGACTTTGAC 
CAGAGAGTAA TGGAGCATTT CATCAAGCTC TACAAGAAGA AAACCGGCAA GGATGTTAGA 
GCGGATAAAA GAGCTGTACA GAAACTTCGC AGAGAGGTGG AGAAAGCAAA GAGGGCATTG 
TCGGCCCAGC ATCAATCCAG AATTGAAATC GAATCTTTCT TTGAAGGGGA GGATTTCTCT 
GAAACTTTGA CAAGAGCCAA GTTTGAGGAG TTGAATATGG ACCTTTTCCG TTCCACTATG 
AAGCCAGTAC AGAAAGTCCT TGATGACTCT GACCTAAAAA AGTCTGATAT TGATGAAATT 
GTGCTTGTCG GGGGCTCTAC TCGTATCCCA AAAATCCAGC AATTGGTTAA AGAATTCTTT 
AATGGCAAGG AGCCTTCCCG TGGTATCAAT CCTGATGAGG CCGTTGCATA TGGTGCAGCT 
GTTCAAGCTG GAGTGCTTTC TGGTGACCAA GATACTGGTG ATTTGGTTCT CCTTGATGTG 
TGCCCACTTA CTCTTGGTAT TGAGACTGTT GGAGGTGTTA TGACAAAACT CATTCCCAGA 
AACACTGTTG TACCCACCAA AAAGTCACAG ATCTTCTCTA CAGCATCCGA CAACCAGCCC 
ACTGTCACTA TCAAAGTTTA TGAAGGTGAG CGTCCACTGA CAAAGGACAA CCAACTCCTG 
GGCACTTTTG ACTTGACCGG AATTCCTCCT GCACCACGTG GAGTTCCCCA GATTGAAGTT 
ACTTTTGAAA TTGACGTGAA TGGTATTCTG CGAGTTACTG CAGAGGACAA GGGCACTGGC 
AACAAGAACA AGATTACCAT TACAAATGAT CAGAACCGTC TGACACCTGA GGAAATTGAG 
AGGATGGTGA CTGATGCAGA GAAATTTGCA GAAGAGGACA AAAAATTGAA GGAGCGCATT 
GACACTCGGA ACGAATTGGA GAGCTATGCT TACTCCTTAC CGAACCAGAT TGGCGATACG 
GCGAAACTTG GGGGAAAACT CTCTCCTGAA GATAAGGCGA CCATTGAGAA GGCTGTGACC 
GAGAAGATTG AGTGGCCAGC TCGCCATCAG GATGCTGATA TAGAAGACTT CAAGGAAAAA 
AAGAAGGAAT TGGAAGAAAT CGTTCAACCA ATTGTAGGCA AACTATATGG TGGAGCTGGT 
GCACCTCCTC CAGAAGGTGC AGAAGGAGCA GAAGAGACTG AGAAGGATGA ATTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165648.1
CDS	43..2019
Translation	MVTMKLFALVLMVSASVFASDDDDKKDDIGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRRIGRTWNDPSVQQDIKYLPFKVIEKKTKPYIVVDVGDQMKTFAPEEISAMVLVKMKETAETYLGRKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKKEGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRADKRAVQKLRREVEKAKRALSAQHQSRIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLDDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNQLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVTDAEKFAEEDKKLKERIDTRNELESYAYSLPNQIGDTAKLGGKLSPEDKATIEKAVTEKIEWPARHQDADIEDFKEKKKELEEIVQPIVGKLYGGAGAPPPEGAEGAEETEKDEL

Target ORF information:

RefSeq Version	NM_001172177.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis heat shock 70 kDa protein 5b (hspa5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172177.1

ORF Insert Sequence:

ATGGTGACCA TGAAGCTGTT TGCCTTGGTG TTGATGGTGT CTGCCAGCGT ATTTGCCTCT 
GATGATGATG ACAAAAAAGA TGACATTGGG ACAGTGGTTG GAATAGATTT GGGCACAACA 
TACTCCTGTG TTGGAGTGTT TAAAAACGGA CGTGTAGAAA TTATTGCCAA TGACCAGGGT 
AACCGTATCA CACCCTCGTA TGTGGCATTT ACCCCAGAAG GTGAGCGTCT CATTGGAGAT 
GCTGCAAAAA ACCAGCTTAC ATCTAATCCA GAGAACACCG TGTTTGATGC CAAACGTCGT 
ATTGGCCGCA CATGGAATGA CCCTTCTGTT CAGCAGGACA TCAAGTACCT GCCATTTAAG 
GTAATTGAGA AGAAAACTAA ACCTTACATT GTAGTCGATG TTGGAGATCA GATGAAAACT 
TTTGCCCCAG AAGAGATTTC TGCTATGGTG CTAGTGAAGA TGAAGGAAAC TGCAGAGACT 
TACTTGGGCA GAAAGGTTAC TCATGCTGTT GTCACTGTAC CTGCTTATTT CAATGATGCA 
CAGCGTCAAG CCACCAAGGA TGCAGGAACT ATTGCAGGGC TGAATGTAAT GAGGATCATC 
AATGAACCCA CTGCTGCGGC CATTGCTTAT GGTCTGGACA AGAAAGAAGG AGAGAAGAAC 
ATCCTTGTGT TTGACTTGGG TGGTGGTACT TTTGATGTTT CCCTACTTAC CATCGATAAT 
GGTGTCTTTG AAGTGGTGGC TACTAATGGA GATACCCATC TTGGTGGAGA AGACTTTGAC 
CAGAGAGTAA TGGAGCATTT CATCAAGCTC TACAAGAAGA AAACCGGCAA GGATGTTAGA 
GCGGATAAAA GAGCTGTACA GAAACTTCGC AGAGAGGTGG AGAAAGCAAA GAGGGCATTG 
TCGGCCCAGC ATCAATCCAG AATTGAAATC GAATCTTTCT TTGAAGGGGA GGATTTCTCT 
GAAACTTTGA CAAGAGCCAA GTTTGAGGAG TTGAATATGG ACCTTTTCCG TTCCACTATG 
AAGCCAGTAC AGAAAGTCCT TGATGACTCT GACCTAAAAA AGTCTGATAT TGATGAAATT 
GTGCTTGTCG GGGGCTCTAC TCGTATCCCA AAAATCCAGC AATTGGTTAA AGAATTCTTT 
AATGGCAAGG AGCCTTCCCG TGGTATCAAT CCTGATGAGG CCGTTGCATA TGGTGCAGCT 
GTTCAAGCTG GAGTGCTTTC TGGTGACCAA GATACTGGTG ATTTGGTTCT CCTTGATGTG 
TGCCCACTTA CTCTTGGTAT TGAGACTGTT GGAGGTGTTA TGACAAAACT CATTCCCAGA 
AACACTGTTG TACCCACCAA AAAGTCACAG ATCTTCTCTA CAGCATCCGA CAACCAGCCC 
ACTGTCACTA TCAAAGTTTA TGAAGGTGAG CGTCCACTGA CAAAGGACAA CCAACTCCTG 
GGCACTTTTG ACTTGACCGG AATTCCTCCT GCACCACGTG GAGTTCCCCA GATTGAAGTT 
ACTTTTGAAA TTGACGTGAA TGGTATTCTG CGAGTTACTG CAGAGGACAA GGGCACTGGC 
AACAAGAACA AGATTACCAT TACAAATGAT CAGAACCGTC TGACACCTGA GGAAATTGAG 
AGGATGGTGA CTGATGCAGA GAAATTTGCA GAAGAGGACA AAAAATTGAA GGAGCGCATT 
GACACTCGGA ACGAATTGGA GAGCTATGCT TACTCCTTAC CGAACCAGAT TGGCGATACG 
GCGAAACTTG GGGGAAAACT CTCTCCTGAA GATAAGGCGA CCATTGAGAA GGCTGTGACC 
GAGAAGATTG AGTGGCCAGC TCGCCATCAG GATGCTGATA TAGAAGACTT CAAGGAAAAA 
AAGAAGGAAT TGGAAGAAAT CGTTCAACCA ATTGTAGGCA AACTATATGG TGGAGCTGGT 
GCACCTCCTC CAGAAGGTGC AGAAGGAGCA GAAGAGACTG AGAAGGATGA ATTGTAG