DBT cDNA ORF clone, Tyto alba(Barn owl)

Gene
Clones
gRNAs

Summary

Gene Symbol	DBT
Entrez Gene ID	104360859
Full Name	dihydrolipoamide branched chain transacylase E2
Gene Type	protein-coding
Organism	Tyto alba(Barn owl)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009966885.1	XP_009965187.1	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial

Pathways from BioSystems
Gene Ontology

The following DBT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DBT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTy03632	XM_009966885.1 Latest version!	Tyto alba dihydrolipoamide branched chain transacylase E2 (DBT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DBT ( XM_009966885.1 ) cDNA ORF clone, Tyto alba(Barn owl) -> XP_009965187.1 Tyto alba dihydrolipoamide branched chain transacylase E2 (DBT), mRNA.

Price & Availability↑

CloneID	OTy03632
Clone ID Related Accession (Same CDS sequence)	XM_009966885.1
Accession Version	XM_009966885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Tyto alba(Barn owl)
Product	lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009998556.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tyto alba Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGTTAAAAA CACATGGAAA GCATTATGAA ACTTCACATT TAGAAATAGT TCAGAGATTT 
GATGTGCTCC TTGTGATCAT CTTCACAGGT GTGCTGTTTT CTGGCTCCTC TAAGAAGATA 
ATATCTAAAT TTTACAGTTT TGAGCTACTT GAGGTTTGCA CTCACCGTGT TAGATCATAC 
AGCAGCATTC ACTTTATCAA ATCAAAATAT GTGTGCGTGT TTGACAGATC TGCCCTCAAG 
TTCAGTCATC AACAGCGATT ATTCAGAACA TCTGCTGTTT CATGTGGCCA AATTGTCCAG 
TTCAAGCTTT CTGACATTGG AGAAGGGATT ACAGAGGTGA CTGTAAAGGA ATGGTATATA 
AAAGAAGGTG ATAGCGTGTC TCAGTTTGAT AGCATCTGTG AAGTACAAAG TGATAAAGCT 
TCCGTCACTA TTACTAGTCG TTATGATGGC ATCATTAGGA AACTCCATTA CAATTTAGAT 
GAAATTGCTT ATGTTGGAAA ACCATTAGTG GACATTGAAA TTGATGCTTC AAAAGGTGTT 
GCCCCAGAAG AAGATGTTGT TGAAACACCT CCTATGTCTC ATGAAGAGCA CACTCACCAA 
GAGATAAAAG GTCATAAAAC ATTAGCAACC CCCGCAGTTC GTCGTCTGGC CATGGAGAAC 
AATATTAAAT TGAGTGAAGT TGTTGGAACA GGCAAAGATA ACAGAATCCT TAAAGAGGAC 
ATACTCAATT ACCTGGCCAA ACAAACAGGA GCTATTTTAC CTCCATCACC GAAGGCTGAA 
ATTATCCCAC CTTTGCCAAA ATCAGAGACT GTGCCAGCTC TGGACAAAGC ACGCAAAATT 
CCTGTACCTG TTTCCAGACC CATTGTGTTT TCAGGAAAAG ATAAAACTGA ACCTGTAACA 
GGTTTTCAAA AGGCAATGGT AAAGACTATG AGTGCAGCCC TGAAGATACC TCACTTTGGT 
TATTGTGATG AGATTGATTT GACTCAGCTT GTTCAGTTGC GAGAAGAACT AAAACCTCTA 
GCACAAATTC GTGGAGTTAA GCTCTCCTTT ATGCCTTTCT TCATAAAGGC AGCCTCTCTG 
GGATTATTGC AGTATCCCAT TCTTAATGCT TCTTTGGATG AAAGCTGTCA AAATGTCACA 
TACAAGGCTT CGCACAACAT TGGAGTTGCT ATGGACACAG AGCAAGGTTT AATTGTCCCA 
AATGTGAAAA ATGTTCAAGT CTGTAGTGTA TTTGAGATTG CTTCTGAATT AAATCGCCTA 
CAGTCCTTGG GCTCTGCAGG CCAACTGGGA ACAAATGATC TCACTGGGGG AACATTCACC 
CTTTCGAATA TTGGCACAAT TGGTGGCACT TACACCAAAC CAGTGATACT ACCTCCTGAA 
GTGGCTATTG GAGCACTTGG AAAGATACAG GTTCTTCCTC GGTTTAATGG AAAAGGTGAA 
GTATTTAAAG CACAGATAAT GAATGTGAGT TGGTCAGCTG ATCACCGCAT CATTGATGGA 
GCTACAATGG CCCGGTTTTC TAACTTATGG AAATCTTACT TGGAGAACCC TGCTTCGATG 
CTGCTAGACC TTAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009965187.1
CDS	1..1578
Translation	MLKTHGKHYETSHLEIVQRFDVLLVIIFTGVLFSGSSKKIISKFYSFELLEVCTHRVRSYSSIHFIKSKYVCVFDRSALKFSHQQRLFRTSAVSCGQIVQFKLSDIGEGITEVTVKEWYIKEGDSVSQFDSICEVQSDKASVTITSRYDGIIRKLHYNLDEIAYVGKPLVDIEIDASKGVAPEEDVVETPPMSHEEHTHQEIKGHKTLATPAVRRLAMENNIKLSEVVGTGKDNRILKEDILNYLAKQTGAILPPSPKAEIIPPLPKSETVPALDKARKIPVPVSRPIVFSGKDKTEPVTGFQKAMVKTMSAALKIPHFGYCDEIDLTQLVQLREELKPLAQIRGVKLSFMPFFIKAASLGLLQYPILNASLDESCQNVTYKASHNIGVAMDTEQGLIVPNVKNVQVCSVFEIASELNRLQSLGSAGQLGTNDLTGGTFTLSNIGTIGGTYTKPVILPPEVAIGALGKIQVLPRFNGKGEVFKAQIMNVSWSADHRIIDGATMARFSNLWKSYLENPASMLLDLK

Target ORF information:

RefSeq Version	XM_009966885.1
Organism	Tyto alba(Barn owl)
Definition	Tyto alba dihydrolipoamide branched chain transacylase E2 (DBT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009966885.1

ORF Insert Sequence:

ATGTTAAAAA CACATGGAAA GCATTATGAA ACTTCACATT TAGAAATAGT TCAGAGATTT 
GATGTGCTCC TTGTGATCAT CTTCACAGGT GTGCTGTTTT CTGGCTCCTC TAAGAAGATA 
ATATCTAAAT TTTACAGTTT TGAGCTACTT GAGGTTTGCA CTCACCGTGT TAGATCATAC 
AGCAGCATTC ACTTTATCAA ATCAAAATAT GTGTGCGTGT TTGACAGATC TGCCCTCAAG 
TTCAGTCATC AACAGCGATT ATTCAGAACA TCTGCTGTTT CATGTGGCCA AATTGTCCAG 
TTCAAGCTTT CTGACATTGG AGAAGGGATT ACAGAGGTGA CTGTAAAGGA ATGGTATATA 
AAAGAAGGTG ATAGCGTGTC TCAGTTTGAT AGCATCTGTG AAGTACAAAG TGATAAAGCT 
TCCGTCACTA TTACTAGTCG TTATGATGGC ATCATTAGGA AACTCCATTA CAATTTAGAT 
GAAATTGCTT ATGTTGGAAA ACCATTAGTG GACATTGAAA TTGATGCTTC AAAAGGTGTT 
GCCCCAGAAG AAGATGTTGT TGAAACACCT CCTATGTCTC ATGAAGAGCA CACTCACCAA 
GAGATAAAAG GTCATAAAAC ATTAGCAACC CCCGCAGTTC GTCGTCTGGC CATGGAGAAC 
AATATTAAAT TGAGTGAAGT TGTTGGAACA GGCAAAGATA ACAGAATCCT TAAAGAGGAC 
ATACTCAATT ACCTGGCCAA ACAAACAGGA GCTATTTTAC CTCCATCACC GAAGGCTGAA 
ATTATCCCAC CTTTGCCAAA ATCAGAGACT GTGCCAGCTC TGGACAAAGC ACGCAAAATT 
CCTGTACCTG TTTCCAGACC CATTGTGTTT TCAGGAAAAG ATAAAACTGA ACCTGTAACA 
GGTTTTCAAA AGGCAATGGT AAAGACTATG AGTGCAGCCC TGAAGATACC TCACTTTGGT 
TATTGTGATG AGATTGATTT GACTCAGCTT GTTCAGTTGC GAGAAGAACT AAAACCTCTA 
GCACAAATTC GTGGAGTTAA GCTCTCCTTT ATGCCTTTCT TCATAAAGGC AGCCTCTCTG 
GGATTATTGC AGTATCCCAT TCTTAATGCT TCTTTGGATG AAAGCTGTCA AAATGTCACA 
TACAAGGCTT CGCACAACAT TGGAGTTGCT ATGGACACAG AGCAAGGTTT AATTGTCCCA 
AATGTGAAAA ATGTTCAAGT CTGTAGTGTA TTTGAGATTG CTTCTGAATT AAATCGCCTA 
CAGTCCTTGG GCTCTGCAGG CCAACTGGGA ACAAATGATC TCACTGGGGG AACATTCACC 
CTTTCGAATA TTGGCACAAT TGGTGGCACT TACACCAAAC CAGTGATACT ACCTCCTGAA 
GTGGCTATTG GAGCACTTGG AAAGATACAG GTTCTTCCTC GGTTTAATGG AAAAGGTGAA 
GTATTTAAAG CACAGATAAT GAATGTGAGT TGGTCAGCTG ATCACCGCAT CATTGATGGA 
GCTACAATGG CCCGGTTTTC TAACTTATGG AAATCTTACT TGGAGAACCC TGCTTCGATG 
CTGCTAGACC TTAAATAA