LOC101361307 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101361307
Entrez Gene ID	101361307
Full Name	defensin beta 112
Synonyms	DEFB112
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012559165.1	XP_012414619.1	beta-defensin 112
XM_012559165.2	XP_012414619.1	beta-defensin 112

Pathways from BioSystems
Gene Ontology

The following LOC101361307 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101361307 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTr186997	XM_012559165.2 Latest version!	Trichechus manatus latirostris defensin beta 112 (LOC101361307), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
View Old Accession Versions >>
OTr186997	XM_012559165.1	Trichechus manatus latirostris defensin, beta 112 (LOC101361307), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101361307 ( XM_012559165.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_012414619.1 Trichechus manatus latirostris defensin, beta 112 (LOC101361307), mRNA.

Price & Availability↑

CloneID	OTr186997
Clone ID Related Accession (Same CDS sequence)	XM_012559165.1 , XM_012559165.2
Accession Version	XM_012559165.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	beta-defensin 112
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444151.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTGTATAT GGAAGCCTGA GAAACTATAC TCAAAGATGC ATAATTCTTC TATAATATTT 
GAAAAGACAC AATATGAGAC AGAGGAAAAG AACACAGCCA GAGGTAAAGA ACATCATGCT 
GTCTTTAACA TGTGGGATGC ATGTATAAAG CTCAGAGGTC AATGTAAAAA ACATTGTGGT 
GAAAATGAAT ATAGGATTGC ATATTGTGCA AGACATACAG TTCATTGCTG CATGAAAAGA 
TGTAAACCTG TGGAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012414619.1
CDS	1..261
Translation	MCIWKPEKLYSKMHNSSIIFEKTQYETEEKNTARGKEHHAVFNMWDACIKLRGQCKKHCGENEYRIAYCARHTVHCCMKRCKPVEK

Target ORF information:

RefSeq Version	XM_012559165.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris defensin, beta 112 (LOC101361307), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012559165.1

ORF Insert Sequence:

ATGTGTATAT GGAAGCCTGA GAAACTATAC TCAAAGATGC ATAATTCTTC TATAATATTT 
GAAAAGACAC AATATGAGAC AGAGGAAAAG AACACAGCCA GAGGTAAAGA ACATCATGCT 
GTCTTTAACA TGTGGGATGC ATGTATAAAG CTCAGAGGTC AATGTAAAAA ACATTGTGGT 
GAAAATGAAT ATAGGATTGC ATATTGTGCA AGACATACAG TTCATTGCTG CATGAAAAGA 
TGTAAACCTG TGGAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101361307 ( XM_012559165.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_012414619.1 Trichechus manatus latirostris defensin beta 112 (LOC101361307), mRNA.

Price & Availability↑

CloneID	OTr186997
Clone ID Related Accession (Same CDS sequence)	XM_012559165.1 , XM_012559165.2
Accession Version	XM_012559165.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 261bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	beta-defensin 112
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004444151.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 25, 2018 this sequence version replaced XM_012559165.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGTGTATAT GGAAGCCTGA GAAACTATAC TCAAAGATGC ATAATTCTTC TATAATATTT 
GAAAAGACAC AATATGAGAC AGAGGAAAAG AACACAGCCA GAGGTAAAGA ACATCATGCT 
GTCTTTAACA TGTGGGATGC ATGTATAAAG CTCAGAGGTC AATGTAAAAA ACATTGTGGT 
GAAAATGAAT ATAGGATTGC ATATTGTGCA AGACATACAG TTCATTGCTG CATGAAAAGA 
TGTAAACCTG TGGAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012414619.1
CDS	243..503
Translation	MCIWKPEKLYSKMHNSSIIFEKTQYETEEKNTARGKEHHAVFNMWDACIKLRGQCKKHCGENEYRIAYCARHTVHCCMKRCKPVEK

Target ORF information:

RefSeq Version	XM_012559165.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris defensin beta 112 (LOC101361307), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012559165.2

ORF Insert Sequence:

ATGTGTATAT GGAAGCCTGA GAAACTATAC TCAAAGATGC ATAATTCTTC TATAATATTT 
GAAAAGACAC AATATGAGAC AGAGGAAAAG AACACAGCCA GAGGTAAAGA ACATCATGCT 
GTCTTTAACA TGTGGGATGC ATGTATAAAG CTCAGAGGTC AATGTAAAAA ACATTGTGGT 
GAAAATGAAT ATAGGATTGC ATATTGTGCA AGACATACAG TTCATTGCTG CATGAAAAGA 
TGTAAACCTG TGGAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.