LOC101342577 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101342577
Entrez Gene ID	101342577
Full Name	TATA-box binding protein associated factor 2
Synonyms	TAF2
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004372974.3	XP_004373031.1	transcription initiation factor TFIID subunit 2 isoform X1
XM_004372974.2	XP_004373031.1	transcription initiation factor TFIID subunit 2 isoform X1
XM_023728169.1	XP_023583937.1	transcription initiation factor TFIID subunit 2 isoform X2
XM_012554539.1	XP_012409993.1	transcription initiation factor TFIID subunit 2 isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC101342577 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101342577 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTr216843	XM_023728169.1 Latest version!	Trichechus manatus latirostris TATA-box binding protein associated factor 2 (LOC101342577), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OTr169398	XM_004372974.3 Latest version!	Trichechus manatus latirostris TATA-box binding protein associated factor 2 (LOC101342577), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OTr169399	XM_012554539.1 Latest version!	Trichechus manatus latirostris TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa (LOC101342577), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OTr169398	XM_004372974.2	Trichechus manatus latirostris TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa (LOC101342577), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101342577 ( XM_023728169.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_023583937.1 Trichechus manatus latirostris TATA-box binding protein associated factor 2 (LOC101342577), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTr216843
Clone ID Related Accession (Same CDS sequence)	XM_023728169.1
Accession Version	XM_023728169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	transcription initiation factor TFIID subunit 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443958.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTTC TCCTTGCTTT GCGTTTTCAG GTGACTCATT TTTGTTTACC CCAACTTCTT 
CCATTGCTTA AACATACTAC GTCATATCTT CATGAAGTCT TTGAATTTTA TGAGGAAATT 
CTTACATGTC GATACCCATA CTCCTGTTTT AAAACTGTGT TCATCGATGA GGCTTATGTT 
GAAGTGGCTG CTTATGCTTC CATGAGCATT TTTAGCACAA ATCTTTTACA CAGTGCCATG 
ATTATAGATG AGACACCTTT GACTAGAAGG TGTTTAGCCC AGTCCTTGGC CCAGCAGTTT 
TTTGGATGTT TCATATCCAG AATGTCTTGG TCTGATGAAT GGGTGCTGAA GGGAATTTCA 
GGCTATATTT ATGGACTTTG GATGAAAAAA ACTTTTGGTG TTAATGAGTA CCGCCACTGG 
ATTAAGGAGG AACTAGACAA AATAGTGGCG TATGAATTGA AGACTGGTGG AGTTTTATTG 
CATCCCATTT TTGGTGGAGG GAAAGAGAAG GATAATCCAG CATCCCACCT ACACTTTTCA 
ATAAAGCATC CACATACACT CTCCTGGGAA TATTACACTA TGTTCCAGTG TAAAGCTCAC 
CTTGTGATGA GATTGATTGA AAATAGGATC AGTATGGAAT TTATGCTGCA AGTTTTCAAT 
AAACTGCTAA GTCTGGCTAG TACTGCTTCA TCTCAGAAGT TCCAGTCACA TATGTGGAGT 
CAGATGTTGG TTTCCACATC TGGGTTTTTG AAGTCCATCT CCAATGTCTC TGGCAAAGAC 
ATCCAGCCTT TAATAAAGCA GTGGGTAGAC CAGAGTGGAG TAGTAAAATT TTATGGAAGT 
TTTGCATTTA ATAGAAAACG AAATGTCTTG GAATTGGAAA TAAAACAAGA TTATACATCT 
CCTGGAACTC AGAAATATGT GGGACCACTT AAAGTAACAG TGCAGGAGTT AGATGGATCC 
TTCAATCATA CATTGCAAAT TGAAGAAAAC AGCCTTAAAC ATGATATACC CTGCCATTCC 
AAAAGCAGAC GAAATAAGAA GAAAAAGATT CCGCTGATGA ATGGAGAAGA AGTTGACATG 
GATCTTTCTG CAATGGATGC TGATTCCCCG TTACTGTGGA TAAGGATAGA CCCAGATATG 
TCAGTACTGA GGAAGGTAGA ATTTGAACAA GCAGATTTTA TGTGGCAGTA CCAACTCCGC 
TATGAGAGGG ATGTTGTTGC ACAGCAGGAA TCCATCTTAG CCTTGGAGAA GTTCCCTACT 
CCAGCCTCTC GGCTTGCACT CACTGACATA TTAGACCAAG AACAGTGTTT CTACCGAGTA 
AGAATGTCAG CTTGCTTCTG TCTTGCGAAG ATTGCAAATT CCATGGTGAG CACATGGACA 
GGACCACCAG CTATGAAGTC ACTCTTCACT AGAATGTTTT GTTGTAAAAC TTGTCCAAAC 
ATTGTGAAAA CAAACAACTT TTTGAGCTTT CAAAGTTATT TTCTACAGAA GACTATGCCA 
GTTGCAATGG CTTTGTTGAG AGATGTTCAT AACCTCTGTC CCAAAGAAGT CTTAACGTTT 
ATTTTAGACT TAATCAAGTA TAATGATAAC AGAAAAAATA AGTTTTCAGA TAACTATTAT 
CGTGCAGAAA TGATTGATGC CCTTGCCAAC TCTGTTACAC CTGCAGTCAG TGTGAATAAT 
GAAGTTAGAA CTTTGGATAA CTTGAATCCT GATGTGCGAC TCATTCTTGA AGAGATCACC 
AGGTTTTTGA ATATGGAAAA ACTTCTTCCA AGTTATAGAC ATACCATCAC TGTTAGTTGT 
TTGAGAGCCA TACGGGTGCT TCAGAAGAAT GGACATGTGC CAAGCGATCC AGCTCTTTTT 
AAGTCTTATG CTGAATATGG CCATTTTGTG GACATTAGGA TAGCAGCTTT GGAAGCAGTT 
GTTGATTATA CTAAAGTGGA CAGGAGTTAT GAAGAACTAC AGTGGCTACT TAATATGATT 
CAGACTGATC CTGTACCCTA TGTAAGGCAT AAGATTTTAA ACATGTTGAC TAAGAATCCA 
CCTTTTACTA AGAATATGGA ATCTCCTTTA TGCAATGAAG CCCTGGTAGA TCAGCTTTGG 
AAACTTATGA ACTCTGGTAC TTCACATGAC TGGAGGTTAC GGTGTGGTGC TGTGGACTTG 
TACTTCACAC TTTTTGGCCT CAGTAGACCT TCCTGCTTAC CTTTGCCAGA GCTTGGGTTG 
GTTCTTAATT TAAAGGAGAA AAAAGCTGTG TTGAATCCTA CCATAATTCC AGAGGCAGTA 
GCAGGCAACC AGGAAGTTGC GAGTAATCCA AGCAGTCACC CACAGCTAGT TGGATTTCAG 
AACACAGAAG ATGATCACCT TGCCAAGGAA GCATCATGTA ATATATCAGC TCATCAGCAG 
GGAGTGAAGA GGAAGGCTGA TACACCACTG GGGTCCCCAC TAGAACCTGG TCAAATACTG 
GAGAAGAATG AGGATAGCAG TAAAGTCAAA CTCAAAATCA GATTCTCAAA TTCTCAAGAT 
GAGGAGGAAA TTGATATGGA TACTGTTCAT GATAGCCAGG CTTTCATTTC TCATCATTTG 
AACATGCTGG AAAGGCCATC AACTCCAGGG CTGTCTAAAT ATCGGCCAGC CAGTTCCCGG 
TCGGCTTTAG TGCCCCAGCA CTCATCAGGC TGCGATGGCA CACCCACCAC AAAACCCCCG 
TGGAGTATGG AGCTTGCACG TAAAGGAACA GGGAAAGAAC AGTCACCCTT GGAGATGAGT 
ATGCATCCAG CAGTGGCACC TCCACTCTCA GTGTTTTCTA AGGAGTCTAC CTCCTCCAAA 
CACAGTGACC ACCATCACCA CCATCACCAC GAGCACAAGA AAAAGAAGAA GAAGCACAAG 
CATAAGCACA AGCACAAGCA TAAGCATGAC AGCAAAGAAA AGGACAAGGA GCCTTTTACT 
TACCACAGCC CCGCCAGTGG CAGGTCTATT CGTTCCCCCT CCCTTTCAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023583937.1
CDS	2095..5088
Translation	MIFLLALRFQVTHFCLPQLLPLLKHTTSYLHEVFEFYEEILTCRYPYSCFKTVFIDEAYVEVAAYASMSIFSTNLLHSAMIIDETPLTRRCLAQSLAQQFFGCFISRMSWSDEWVLKGISGYIYGLWMKKTFGVNEYRHWIKEELDKIVAYELKTGGVLLHPIFGGGKEKDNPASHLHFSIKHPHTLSWEYYTMFQCKAHLVMRLIENRISMEFMLQVFNKLLSLASTASSQKFQSHMWSQMLVSTSGFLKSISNVSGKDIQPLIKQWVDQSGVVKFYGSFAFNRKRNVLELEIKQDYTSPGTQKYVGPLKVTVQELDGSFNHTLQIEENSLKHDIPCHSKSRRNKKKKIPLMNGEEVDMDLSAMDADSPLLWIRIDPDMSVLRKVEFEQADFMWQYQLRYERDVVAQQESILALEKFPTPASRLALTDILDQEQCFYRVRMSACFCLAKIANSMVSTWTGPPAMKSLFTRMFCCKTCPNIVKTNNFLSFQSYFLQKTMPVAMALLRDVHNLCPKEVLTFILDLIKYNDNRKNKFSDNYYRAEMIDALANSVTPAVSVNNEVRTLDNLNPDVRLILEEITRFLNMEKLLPSYRHTITVSCLRAIRVLQKNGHVPSDPALFKSYAEYGHFVDIRIAALEAVVDYTKVDRSYEELQWLLNMIQTDPVPYVRHKILNMLTKNPPFTKNMESPLCNEALVDQLWKLMNSGTSHDWRLRCGAVDLYFTLFGLSRPSCLPLPELGLVLNLKEKKAVLNPTIIPEAVAGNQEVASNPSSHPQLVGFQNTEDDHLAKEASCNISAHQQGVKRKADTPLGSPLEPGQILEKNEDSSKVKLKIRFSNSQDEEEIDMDTVHDSQAFISHHLNMLERPSTPGLSKYRPASSRSALVPQHSSGCDGTPTTKPPWSMELARKGTGKEQSPLEMSMHPAVAPPLSVFSKESTSSKHSDHHHHHHHEHKKKKKKHKHKHKHKHKHDSKEKDKEPFTYHSPASGRSIRSPSLSD

Target ORF information:

RefSeq Version	XM_023728169.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris TATA-box binding protein associated factor 2 (LOC101342577), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023728169.1

ORF Insert Sequence:

ATGATTTTTC TCCTTGCTTT GCGTTTTCAG GTGACTCATT TTTGTTTACC CCAACTTCTT 
CCATTGCTTA AACATACTAC GTCATATCTT CATGAAGTCT TTGAATTTTA TGAGGAAATT 
CTTACATGTC GATACCCATA CTCCTGTTTT AAAACTGTGT TCATCGATGA GGCTTATGTT 
GAAGTGGCTG CTTATGCTTC CATGAGCATT TTTAGCACAA ATCTTTTACA CAGTGCCATG 
ATTATAGATG AGACACCTTT GACTAGAAGG TGTTTAGCCC AGTCCTTGGC CCAGCAGTTT 
TTTGGATGTT TCATATCCAG AATGTCTTGG TCTGATGAAT GGGTGCTGAA GGGAATTTCA 
GGCTATATTT ATGGACTTTG GATGAAAAAA ACTTTTGGTG TTAATGAGTA CCGCCACTGG 
ATTAAGGAGG AACTAGACAA AATAGTGGCG TATGAATTGA AGACTGGTGG AGTTTTATTG 
CATCCCATTT TTGGTGGAGG GAAAGAGAAG GATAATCCAG CATCCCACCT ACACTTTTCA 
ATAAAGCATC CACATACACT CTCCTGGGAA TATTACACTA TGTTCCAGTG TAAAGCTCAC 
CTTGTGATGA GATTGATTGA AAATAGGATC AGTATGGAAT TTATGCTGCA AGTTTTCAAT 
AAACTGCTAA GTCTGGCTAG TACTGCTTCA TCTCAGAAGT TCCAGTCACA TATGTGGAGT 
CAGATGTTGG TTTCCACATC TGGGTTTTTG AAGTCCATCT CCAATGTCTC TGGCAAAGAC 
ATCCAGCCTT TAATAAAGCA GTGGGTAGAC CAGAGTGGAG TAGTAAAATT TTATGGAAGT 
TTTGCATTTA ATAGAAAACG AAATGTCTTG GAATTGGAAA TAAAACAAGA TTATACATCT 
CCTGGAACTC AGAAATATGT GGGACCACTT AAAGTAACAG TGCAGGAGTT AGATGGATCC 
TTCAATCATA CATTGCAAAT TGAAGAAAAC AGCCTTAAAC ATGATATACC CTGCCATTCC 
AAAAGCAGAC GAAATAAGAA GAAAAAGATT CCGCTGATGA ATGGAGAAGA AGTTGACATG 
GATCTTTCTG CAATGGATGC TGATTCCCCG TTACTGTGGA TAAGGATAGA CCCAGATATG 
TCAGTACTGA GGAAGGTAGA ATTTGAACAA GCAGATTTTA TGTGGCAGTA CCAACTCCGC 
TATGAGAGGG ATGTTGTTGC ACAGCAGGAA TCCATCTTAG CCTTGGAGAA GTTCCCTACT 
CCAGCCTCTC GGCTTGCACT CACTGACATA TTAGACCAAG AACAGTGTTT CTACCGAGTA 
AGAATGTCAG CTTGCTTCTG TCTTGCGAAG ATTGCAAATT CCATGGTGAG CACATGGACA 
GGACCACCAG CTATGAAGTC ACTCTTCACT AGAATGTTTT GTTGTAAAAC TTGTCCAAAC 
ATTGTGAAAA CAAACAACTT TTTGAGCTTT CAAAGTTATT TTCTACAGAA GACTATGCCA 
GTTGCAATGG CTTTGTTGAG AGATGTTCAT AACCTCTGTC CCAAAGAAGT CTTAACGTTT 
ATTTTAGACT TAATCAAGTA TAATGATAAC AGAAAAAATA AGTTTTCAGA TAACTATTAT 
CGTGCAGAAA TGATTGATGC CCTTGCCAAC TCTGTTACAC CTGCAGTCAG TGTGAATAAT 
GAAGTTAGAA CTTTGGATAA CTTGAATCCT GATGTGCGAC TCATTCTTGA AGAGATCACC 
AGGTTTTTGA ATATGGAAAA ACTTCTTCCA AGTTATAGAC ATACCATCAC TGTTAGTTGT 
TTGAGAGCCA TACGGGTGCT TCAGAAGAAT GGACATGTGC CAAGCGATCC AGCTCTTTTT 
AAGTCTTATG CTGAATATGG CCATTTTGTG GACATTAGGA TAGCAGCTTT GGAAGCAGTT 
GTTGATTATA CTAAAGTGGA CAGGAGTTAT GAAGAACTAC AGTGGCTACT TAATATGATT 
CAGACTGATC CTGTACCCTA TGTAAGGCAT AAGATTTTAA ACATGTTGAC TAAGAATCCA 
CCTTTTACTA AGAATATGGA ATCTCCTTTA TGCAATGAAG CCCTGGTAGA TCAGCTTTGG 
AAACTTATGA ACTCTGGTAC TTCACATGAC TGGAGGTTAC GGTGTGGTGC TGTGGACTTG 
TACTTCACAC TTTTTGGCCT CAGTAGACCT TCCTGCTTAC CTTTGCCAGA GCTTGGGTTG 
GTTCTTAATT TAAAGGAGAA AAAAGCTGTG TTGAATCCTA CCATAATTCC AGAGGCAGTA 
GCAGGCAACC AGGAAGTTGC GAGTAATCCA AGCAGTCACC CACAGCTAGT TGGATTTCAG 
AACACAGAAG ATGATCACCT TGCCAAGGAA GCATCATGTA ATATATCAGC TCATCAGCAG 
GGAGTGAAGA GGAAGGCTGA TACACCACTG GGGTCCCCAC TAGAACCTGG TCAAATACTG 
GAGAAGAATG AGGATAGCAG TAAAGTCAAA CTCAAAATCA GATTCTCAAA TTCTCAAGAT 
GAGGAGGAAA TTGATATGGA TACTGTTCAT GATAGCCAGG CTTTCATTTC TCATCATTTG 
AACATGCTGG AAAGGCCATC AACTCCAGGG CTGTCTAAAT ATCGGCCAGC CAGTTCCCGG 
TCGGCTTTAG TGCCCCAGCA CTCATCAGGC TGCGATGGCA CACCCACCAC AAAACCCCCG 
TGGAGTATGG AGCTTGCACG TAAAGGAACA GGGAAAGAAC AGTCACCCTT GGAGATGAGT 
ATGCATCCAG CAGTGGCACC TCCACTCTCA GTGTTTTCTA AGGAGTCTAC CTCCTCCAAA 
CACAGTGACC ACCATCACCA CCATCACCAC GAGCACAAGA AAAAGAAGAA GAAGCACAAG 
CATAAGCACA AGCACAAGCA TAAGCATGAC AGCAAAGAAA AGGACAAGGA GCCTTTTACT 
TACCACAGCC CCGCCAGTGG CAGGTCTATT CGTTCCCCCT CCCTTTCAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101342577 ( XM_004372974.3 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004373031.1 Trichechus manatus latirostris TATA-box binding protein associated factor 2 (LOC101342577), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTr169398
Clone ID Related Accession (Same CDS sequence)	XM_004372974.3 , XM_004372974.2
Accession Version	XM_004372974.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	transcription initiation factor TFIID subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443958.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 25, 2018 this sequence version replaced XM_004372974.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGA CTGGTTTAGA GCCCGCCAGA ATGAACAGGA AGAAAGGAGA CAAGGGGTTT 
GAAAGCCCAA GGCCATATAA ATTAACCCAT CAGGTGGTCT GCATCAACAA CATAAACTTC 
CAGAGAAAAT CTGTTGTGGG ATTTGTAGAA CTGACTATAT TTCCCACAGT TGCAAACTTG 
AATAGAATCA AGTTGAACAG CAAACAGTGC AGAATATACC GAGTAAGGGT CAACGACTTA 
GAGGCTGCCT TTATTTATAA TGATCCAACC TTGGAAGTTT GTCACAATGA ATCGAAACAG 
AGAAATCTCA ATTATTTTTC CAATGCTTAT GCAGCTGCAG TTAGTGCTGT GGATCCTGAT 
GCGGGAAATG GAGAACTTTG CATTAAGGTT CCCTCAGAGC TTTGGAAACA TGTTGATGAG 
TTAAAGGTCT TGAAGATACA CATCAATTTT TCTTTGGATC AGCCAAAAGG AGGTCTTCAT 
TTTGTGGTAC CCAGTGTGGA GGGAAGCATG GCTGAGAGAG GTGCTCATGT TTTCTCTTGT 
GGGTATCAAA ATTCCACAAG ATTTTGGTTC CCATGTGTTG ATTCATACTC TGAATTATGT 
ACATGGAAAT TAGAATTCAC AGTAGATGCT GCAATGGTGG CTGTTTCCAA TGGAGATTTG 
GTGGAGACAG TGTATACCCA TGATATGAGG AAGAAGACCT TCCATTACAT GCTTACCATT 
CCAACAGCAG CGTCAAATAT CTCCTTGGCC ATTGGACCTT TTGAAATACT TGTAGATCCA 
TATATGCATG AGGTGACTCA TTTTTGTTTA CCCCAACTTC TTCCATTGCT TAAACATACT 
ACGTCATATC TTCATGAAGT CTTTGAATTT TATGAGGAAA TTCTTACATG TCGATACCCA 
TACTCCTGTT TTAAAACTGT GTTCATCGAT GAGGCTTATG TTGAAGTGGC TGCTTATGCT 
TCCATGAGCA TTTTTAGCAC AAATCTTTTA CACAGTGCCA TGATTATAGA TGAGACACCT 
TTGACTAGAA GGTGTTTAGC CCAGTCCTTG GCCCAGCAGT TTTTTGGATG TTTCATATCC 
AGAATGTCTT GGTCTGATGA ATGGGTGCTG AAGGGAATTT CAGGCTATAT TTATGGACTT 
TGGATGAAAA AAACTTTTGG TGTTAATGAG TACCGCCACT GGATTAAGGA GGAACTAGAC 
AAAATAGTGG CGTATGAATT GAAGACTGGT GGAGTTTTAT TGCATCCCAT TTTTGGTGGA 
GGGAAAGAGA AGGATAATCC AGCATCCCAC CTACACTTTT CAATAAAGCA TCCACATACA 
CTCTCCTGGG AATATTACAC TATGTTCCAG TGTAAAGCTC ACCTTGTGAT GAGATTGATT 
GAAAATAGGA TCAGTATGGA ATTTATGCTG CAAGTTTTCA ATAAACTGCT AAGTCTGGCT 
AGTACTGCTT CATCTCAGAA GTTCCAGTCA CATATGTGGA GTCAGATGTT GGTTTCCACA 
TCTGGGTTTT TGAAGTCCAT CTCCAATGTC TCTGGCAAAG ACATCCAGCC TTTAATAAAG 
CAGTGGGTAG ACCAGAGTGG AGTAGTAAAA TTTTATGGAA GTTTTGCATT TAATAGAAAA 
CGAAATGTCT TGGAATTGGA AATAAAACAA GATTATACAT CTCCTGGAAC TCAGAAATAT 
GTGGGACCAC TTAAAGTAAC AGTGCAGGAG TTAGATGGAT CCTTCAATCA TACATTGCAA 
ATTGAAGAAA ACAGCCTTAA ACATGATATA CCCTGCCATT CCAAAAGCAG ACGAAATAAG 
AAGAAAAAGA TTCCGCTGAT GAATGGAGAA GAAGTTGACA TGGATCTTTC TGCAATGGAT 
GCTGATTCCC CGTTACTGTG GATAAGGATA GACCCAGATA TGTCAGTACT GAGGAAGGTA 
GAATTTGAAC AAGCAGATTT TATGTGGCAG TACCAACTCC GCTATGAGAG GGATGTTGTT 
GCACAGCAGG AATCCATCTT AGCCTTGGAG AAGTTCCCTA CTCCAGCCTC TCGGCTTGCA 
CTCACTGACA TATTAGACCA AGAACAGTGT TTCTACCGAG TAAGAATGTC AGCTTGCTTC 
TGTCTTGCGA AGATTGCAAA TTCCATGGTG AGCACATGGA CAGGACCACC AGCTATGAAG 
TCACTCTTCA CTAGAATGTT TTGTTGTAAA ACTTGTCCAA ACATTGTGAA AACAAACAAC 
TTTTTGAGCT TTCAAAGTTA TTTTCTACAG AAGACTATGC CAGTTGCAAT GGCTTTGTTG 
AGAGATGTTC ATAACCTCTG TCCCAAAGAA GTCTTAACGT TTATTTTAGA CTTAATCAAG 
TATAATGATA ACAGAAAAAA TAAGTTTTCA GATAACTATT ATCGTGCAGA AATGATTGAT 
GCCCTTGCCA ACTCTGTTAC ACCTGCAGTC AGTGTGAATA ATGAAGTTAG AACTTTGGAT 
AACTTGAATC CTGATGTGCG ACTCATTCTT GAAGAGATCA CCAGGTTTTT GAATATGGAA 
AAACTTCTTC CAAGTTATAG ACATACCATC ACTGTTAGTT GTTTGAGAGC CATACGGGTG 
CTTCAGAAGA ATGGACATGT GCCAAGCGAT CCAGCTCTTT TTAAGTCTTA TGCTGAATAT 
GGCCATTTTG TGGACATTAG GATAGCAGCT TTGGAAGCAG TTGTTGATTA TACTAAAGTG 
GACAGGAGTT ATGAAGAACT ACAGTGGCTA CTTAATATGA TTCAGACTGA TCCTGTACCC 
TATGTAAGGC ATAAGATTTT AAACATGTTG ACTAAGAATC CACCTTTTAC TAAGAATATG 
GAATCTCCTT TATGCAATGA AGCCCTGGTA GATCAGCTTT GGAAACTTAT GAACTCTGGT 
ACTTCACATG ACTGGAGGTT ACGGTGTGGT GCTGTGGACT TGTACTTCAC ACTTTTTGGC 
CTCAGTAGAC CTTCCTGCTT ACCTTTGCCA GAGCTTGGGT TGGTTCTTAA TTTAAAGGAG 
AAAAAAGCTG TGTTGAATCC TACCATAATT CCAGAGGCAG TAGCAGGCAA CCAGGAAGTT 
GCGAGTAATC CAAGCAGTCA CCCACAGCTA GTTGGATTTC AGAACACATT CTCAAATTCT 
CAAGATGAGG AGGAAATTGA TATGGATACT GTTCATGATA GCCAGGCTTT CATTTCTCAT 
CATTTGAACA TGCTGGAAAG GCCATCAACT CCAGGGCTGT CTAAATATCG GCCAGCCAGT 
TCCCGGTCGG CTTTAGTGCC CCAGCACTCA TCAGGCTGCG ATGGCACACC CACCACAAAA 
CCCCCGTGGA GTATGGAGCT TGCACGTAAA GGAACAGGGA AAGAACAGTC ACCCTTGGAG 
ATGAGTATGC ATCCAGCAGT GGCACCTCCA CTCTCAGTGT TTTCTAAGGA GTCTACCTCC 
TCCAAACACA GTGACCACCA TCACCACCAT CACCACGAGC ACAAGAAAAA GAAGAAGAAG 
CACAAGCATA AGCACAAGCA CAAGCATAAG CATGACAGCA AAGAAAAGGA CAAGGAGCCT 
TTTACTTACC ACAGCCCCGC CAGTGGCAGG TCTATTCGTT CCCCCTCCCT TTCAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004373031.1
CDS	39..3638
Translation	MPPTGLEPARMNRKKGDKGFESPRPYKLTHQVVCINNINFQRKSVVGFVELTIFPTVANLNRIKLNSKQCRIYRVRVNDLEAAFIYNDPTLEVCHNESKQRNLNYFSNAYAAAVSAVDPDAGNGELCIKVPSELWKHVDELKVLKIHINFSLDQPKGGLHFVVPSVEGSMAERGAHVFSCGYQNSTRFWFPCVDSYSELCTWKLEFTVDAAMVAVSNGDLVETVYTHDMRKKTFHYMLTIPTAASNISLAIGPFEILVDPYMHEVTHFCLPQLLPLLKHTTSYLHEVFEFYEEILTCRYPYSCFKTVFIDEAYVEVAAYASMSIFSTNLLHSAMIIDETPLTRRCLAQSLAQQFFGCFISRMSWSDEWVLKGISGYIYGLWMKKTFGVNEYRHWIKEELDKIVAYELKTGGVLLHPIFGGGKEKDNPASHLHFSIKHPHTLSWEYYTMFQCKAHLVMRLIENRISMEFMLQVFNKLLSLASTASSQKFQSHMWSQMLVSTSGFLKSISNVSGKDIQPLIKQWVDQSGVVKFYGSFAFNRKRNVLELEIKQDYTSPGTQKYVGPLKVTVQELDGSFNHTLQIEENSLKHDIPCHSKSRRNKKKKIPLMNGEEVDMDLSAMDADSPLLWIRIDPDMSVLRKVEFEQADFMWQYQLRYERDVVAQQESILALEKFPTPASRLALTDILDQEQCFYRVRMSACFCLAKIANSMVSTWTGPPAMKSLFTRMFCCKTCPNIVKTNNFLSFQSYFLQKTMPVAMALLRDVHNLCPKEVLTFILDLIKYNDNRKNKFSDNYYRAEMIDALANSVTPAVSVNNEVRTLDNLNPDVRLILEEITRFLNMEKLLPSYRHTITVSCLRAIRVLQKNGHVPSDPALFKSYAEYGHFVDIRIAALEAVVDYTKVDRSYEELQWLLNMIQTDPVPYVRHKILNMLTKNPPFTKNMESPLCNEALVDQLWKLMNSGTSHDWRLRCGAVDLYFTLFGLSRPSCLPLPELGLVLNLKEKKAVLNPTIIPEAVAGNQEVASNPSSHPQLVGFQNTFSNSQDEEEIDMDTVHDSQAFISHHLNMLERPSTPGLSKYRPASSRSALVPQHSSGCDGTPTTKPPWSMELARKGTGKEQSPLEMSMHPAVAPPLSVFSKESTSSKHSDHHHHHHHEHKKKKKKHKHKHKHKHKHDSKEKDKEPFTYHSPASGRSIRSPSLSD

Target ORF information:

RefSeq Version	XM_004372974.3
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris TATA-box binding protein associated factor 2 (LOC101342577), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004372974.3

ORF Insert Sequence:

ATGCCGCCGA CTGGTTTAGA GCCCGCCAGA ATGAACAGGA AGAAAGGAGA CAAGGGGTTT 
GAAAGCCCAA GGCCATATAA ATTAACCCAT CAGGTGGTCT GCATCAACAA CATAAACTTC 
CAGAGAAAAT CTGTTGTGGG ATTTGTAGAA CTGACTATAT TTCCCACAGT TGCAAACTTG 
AATAGAATCA AGTTGAACAG CAAACAGTGC AGAATATACC GAGTAAGGGT CAACGACTTA 
GAGGCTGCCT TTATTTATAA TGATCCAACC TTGGAAGTTT GTCACAATGA ATCGAAACAG 
AGAAATCTCA ATTATTTTTC CAATGCTTAT GCAGCTGCAG TTAGTGCTGT GGATCCTGAT 
GCGGGAAATG GAGAACTTTG CATTAAGGTT CCCTCAGAGC TTTGGAAACA TGTTGATGAG 
TTAAAGGTCT TGAAGATACA CATCAATTTT TCTTTGGATC AGCCAAAAGG AGGTCTTCAT 
TTTGTGGTAC CCAGTGTGGA GGGAAGCATG GCTGAGAGAG GTGCTCATGT TTTCTCTTGT 
GGGTATCAAA ATTCCACAAG ATTTTGGTTC CCATGTGTTG ATTCATACTC TGAATTATGT 
ACATGGAAAT TAGAATTCAC AGTAGATGCT GCAATGGTGG CTGTTTCCAA TGGAGATTTG 
GTGGAGACAG TGTATACCCA TGATATGAGG AAGAAGACCT TCCATTACAT GCTTACCATT 
CCAACAGCAG CGTCAAATAT CTCCTTGGCC ATTGGACCTT TTGAAATACT TGTAGATCCA 
TATATGCATG AGGTGACTCA TTTTTGTTTA CCCCAACTTC TTCCATTGCT TAAACATACT 
ACGTCATATC TTCATGAAGT CTTTGAATTT TATGAGGAAA TTCTTACATG TCGATACCCA 
TACTCCTGTT TTAAAACTGT GTTCATCGAT GAGGCTTATG TTGAAGTGGC TGCTTATGCT 
TCCATGAGCA TTTTTAGCAC AAATCTTTTA CACAGTGCCA TGATTATAGA TGAGACACCT 
TTGACTAGAA GGTGTTTAGC CCAGTCCTTG GCCCAGCAGT TTTTTGGATG TTTCATATCC 
AGAATGTCTT GGTCTGATGA ATGGGTGCTG AAGGGAATTT CAGGCTATAT TTATGGACTT 
TGGATGAAAA AAACTTTTGG TGTTAATGAG TACCGCCACT GGATTAAGGA GGAACTAGAC 
AAAATAGTGG CGTATGAATT GAAGACTGGT GGAGTTTTAT TGCATCCCAT TTTTGGTGGA 
GGGAAAGAGA AGGATAATCC AGCATCCCAC CTACACTTTT CAATAAAGCA TCCACATACA 
CTCTCCTGGG AATATTACAC TATGTTCCAG TGTAAAGCTC ACCTTGTGAT GAGATTGATT 
GAAAATAGGA TCAGTATGGA ATTTATGCTG CAAGTTTTCA ATAAACTGCT AAGTCTGGCT 
AGTACTGCTT CATCTCAGAA GTTCCAGTCA CATATGTGGA GTCAGATGTT GGTTTCCACA 
TCTGGGTTTT TGAAGTCCAT CTCCAATGTC TCTGGCAAAG ACATCCAGCC TTTAATAAAG 
CAGTGGGTAG ACCAGAGTGG AGTAGTAAAA TTTTATGGAA GTTTTGCATT TAATAGAAAA 
CGAAATGTCT TGGAATTGGA AATAAAACAA GATTATACAT CTCCTGGAAC TCAGAAATAT 
GTGGGACCAC TTAAAGTAAC AGTGCAGGAG TTAGATGGAT CCTTCAATCA TACATTGCAA 
ATTGAAGAAA ACAGCCTTAA ACATGATATA CCCTGCCATT CCAAAAGCAG ACGAAATAAG 
AAGAAAAAGA TTCCGCTGAT GAATGGAGAA GAAGTTGACA TGGATCTTTC TGCAATGGAT 
GCTGATTCCC CGTTACTGTG GATAAGGATA GACCCAGATA TGTCAGTACT GAGGAAGGTA 
GAATTTGAAC AAGCAGATTT TATGTGGCAG TACCAACTCC GCTATGAGAG GGATGTTGTT 
GCACAGCAGG AATCCATCTT AGCCTTGGAG AAGTTCCCTA CTCCAGCCTC TCGGCTTGCA 
CTCACTGACA TATTAGACCA AGAACAGTGT TTCTACCGAG TAAGAATGTC AGCTTGCTTC 
TGTCTTGCGA AGATTGCAAA TTCCATGGTG AGCACATGGA CAGGACCACC AGCTATGAAG 
TCACTCTTCA CTAGAATGTT TTGTTGTAAA ACTTGTCCAA ACATTGTGAA AACAAACAAC 
TTTTTGAGCT TTCAAAGTTA TTTTCTACAG AAGACTATGC CAGTTGCAAT GGCTTTGTTG 
AGAGATGTTC ATAACCTCTG TCCCAAAGAA GTCTTAACGT TTATTTTAGA CTTAATCAAG 
TATAATGATA ACAGAAAAAA TAAGTTTTCA GATAACTATT ATCGTGCAGA AATGATTGAT 
GCCCTTGCCA ACTCTGTTAC ACCTGCAGTC AGTGTGAATA ATGAAGTTAG AACTTTGGAT 
AACTTGAATC CTGATGTGCG ACTCATTCTT GAAGAGATCA CCAGGTTTTT GAATATGGAA 
AAACTTCTTC CAAGTTATAG ACATACCATC ACTGTTAGTT GTTTGAGAGC CATACGGGTG 
CTTCAGAAGA ATGGACATGT GCCAAGCGAT CCAGCTCTTT TTAAGTCTTA TGCTGAATAT 
GGCCATTTTG TGGACATTAG GATAGCAGCT TTGGAAGCAG TTGTTGATTA TACTAAAGTG 
GACAGGAGTT ATGAAGAACT ACAGTGGCTA CTTAATATGA TTCAGACTGA TCCTGTACCC 
TATGTAAGGC ATAAGATTTT AAACATGTTG ACTAAGAATC CACCTTTTAC TAAGAATATG 
GAATCTCCTT TATGCAATGA AGCCCTGGTA GATCAGCTTT GGAAACTTAT GAACTCTGGT 
ACTTCACATG ACTGGAGGTT ACGGTGTGGT GCTGTGGACT TGTACTTCAC ACTTTTTGGC 
CTCAGTAGAC CTTCCTGCTT ACCTTTGCCA GAGCTTGGGT TGGTTCTTAA TTTAAAGGAG 
AAAAAAGCTG TGTTGAATCC TACCATAATT CCAGAGGCAG TAGCAGGCAA CCAGGAAGTT 
GCGAGTAATC CAAGCAGTCA CCCACAGCTA GTTGGATTTC AGAACACATT CTCAAATTCT 
CAAGATGAGG AGGAAATTGA TATGGATACT GTTCATGATA GCCAGGCTTT CATTTCTCAT 
CATTTGAACA TGCTGGAAAG GCCATCAACT CCAGGGCTGT CTAAATATCG GCCAGCCAGT 
TCCCGGTCGG CTTTAGTGCC CCAGCACTCA TCAGGCTGCG ATGGCACACC CACCACAAAA 
CCCCCGTGGA GTATGGAGCT TGCACGTAAA GGAACAGGGA AAGAACAGTC ACCCTTGGAG 
ATGAGTATGC ATCCAGCAGT GGCACCTCCA CTCTCAGTGT TTTCTAAGGA GTCTACCTCC 
TCCAAACACA GTGACCACCA TCACCACCAT CACCACGAGC ACAAGAAAAA GAAGAAGAAG 
CACAAGCATA AGCACAAGCA CAAGCATAAG CATGACAGCA AAGAAAAGGA CAAGGAGCCT 
TTTACTTACC ACAGCCCCGC CAGTGGCAGG TCTATTCGTT CCCCCTCCCT TTCAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101342577 ( XM_004372974.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_004373031.1 Trichechus manatus latirostris TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa (LOC101342577), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTr169398
Clone ID Related Accession (Same CDS sequence)	XM_004372974.3 , XM_004372974.2
Accession Version	XM_004372974.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	transcription initiation factor TFIID subunit 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443958.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2015 this sequence version replaced XM_004372974.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGA CTGGTTTAGA GCCCGCCAGA ATGAACAGGA AGAAAGGAGA CAAGGGGTTT 
GAAAGCCCAA GGCCATATAA ATTAACCCAT CAGGTGGTCT GCATCAACAA CATAAACTTC 
CAGAGAAAAT CTGTTGTGGG ATTTGTAGAA CTGACTATAT TTCCCACAGT TGCAAACTTG 
AATAGAATCA AGTTGAACAG CAAACAGTGC AGAATATACC GAGTAAGGGT CAACGACTTA 
GAGGCTGCCT TTATTTATAA TGATCCAACC TTGGAAGTTT GTCACAATGA ATCGAAACAG 
AGAAATCTCA ATTATTTTTC CAATGCTTAT GCAGCTGCAG TTAGTGCTGT GGATCCTGAT 
GCGGGAAATG GAGAACTTTG CATTAAGGTT CCCTCAGAGC TTTGGAAACA TGTTGATGAG 
TTAAAGGTCT TGAAGATACA CATCAATTTT TCTTTGGATC AGCCAAAAGG AGGTCTTCAT 
TTTGTGGTAC CCAGTGTGGA GGGAAGCATG GCTGAGAGAG GTGCTCATGT TTTCTCTTGT 
GGGTATCAAA ATTCCACAAG ATTTTGGTTC CCATGTGTTG ATTCATACTC TGAATTATGT 
ACATGGAAAT TAGAATTCAC AGTAGATGCT GCAATGGTGG CTGTTTCCAA TGGAGATTTG 
GTGGAGACAG TGTATACCCA TGATATGAGG AAGAAGACCT TCCATTACAT GCTTACCATT 
CCAACAGCAG CGTCAAATAT CTCCTTGGCC ATTGGACCTT TTGAAATACT TGTAGATCCA 
TATATGCATG AGGTGACTCA TTTTTGTTTA CCCCAACTTC TTCCATTGCT TAAACATACT 
ACGTCATATC TTCATGAAGT CTTTGAATTT TATGAGGAAA TTCTTACATG TCGATACCCA 
TACTCCTGTT TTAAAACTGT GTTCATCGAT GAGGCTTATG TTGAAGTGGC TGCTTATGCT 
TCCATGAGCA TTTTTAGCAC AAATCTTTTA CACAGTGCCA TGATTATAGA TGAGACACCT 
TTGACTAGAA GGTGTTTAGC CCAGTCCTTG GCCCAGCAGT TTTTTGGATG TTTCATATCC 
AGAATGTCTT GGTCTGATGA ATGGGTGCTG AAGGGAATTT CAGGCTATAT TTATGGACTT 
TGGATGAAAA AAACTTTTGG TGTTAATGAG TACCGCCACT GGATTAAGGA GGAACTAGAC 
AAAATAGTGG CGTATGAATT GAAGACTGGT GGAGTTTTAT TGCATCCCAT TTTTGGTGGA 
GGGAAAGAGA AGGATAATCC AGCATCCCAC CTACACTTTT CAATAAAGCA TCCACATACA 
CTCTCCTGGG AATATTACAC TATGTTCCAG TGTAAAGCTC ACCTTGTGAT GAGATTGATT 
GAAAATAGGA TCAGTATGGA ATTTATGCTG CAAGTTTTCA ATAAACTGCT AAGTCTGGCT 
AGTACTGCTT CATCTCAGAA GTTCCAGTCA CATATGTGGA GTCAGATGTT GGTTTCCACA 
TCTGGGTTTT TGAAGTCCAT CTCCAATGTC TCTGGCAAAG ACATCCAGCC TTTAATAAAG 
CAGTGGGTAG ACCAGAGTGG AGTAGTAAAA TTTTATGGAA GTTTTGCATT TAATAGAAAA 
CGAAATGTCT TGGAATTGGA AATAAAACAA GATTATACAT CTCCTGGAAC TCAGAAATAT 
GTGGGACCAC TTAAAGTAAC AGTGCAGGAG TTAGATGGAT CCTTCAATCA TACATTGCAA 
ATTGAAGAAA ACAGCCTTAA ACATGATATA CCCTGCCATT CCAAAAGCAG ACGAAATAAG 
AAGAAAAAGA TTCCGCTGAT GAATGGAGAA GAAGTTGACA TGGATCTTTC TGCAATGGAT 
GCTGATTCCC CGTTACTGTG GATAAGGATA GACCCAGATA TGTCAGTACT GAGGAAGGTA 
GAATTTGAAC AAGCAGATTT TATGTGGCAG TACCAACTCC GCTATGAGAG GGATGTTGTT 
GCACAGCAGG AATCCATCTT AGCCTTGGAG AAGTTCCCTA CTCCAGCCTC TCGGCTTGCA 
CTCACTGACA TATTAGACCA AGAACAGTGT TTCTACCGAG TAAGAATGTC AGCTTGCTTC 
TGTCTTGCGA AGATTGCAAA TTCCATGGTG AGCACATGGA CAGGACCACC AGCTATGAAG 
TCACTCTTCA CTAGAATGTT TTGTTGTAAA ACTTGTCCAA ACATTGTGAA AACAAACAAC 
TTTTTGAGCT TTCAAAGTTA TTTTCTACAG AAGACTATGC CAGTTGCAAT GGCTTTGTTG 
AGAGATGTTC ATAACCTCTG TCCCAAAGAA GTCTTAACGT TTATTTTAGA CTTAATCAAG 
TATAATGATA ACAGAAAAAA TAAGTTTTCA GATAACTATT ATCGTGCAGA AATGATTGAT 
GCCCTTGCCA ACTCTGTTAC ACCTGCAGTC AGTGTGAATA ATGAAGTTAG AACTTTGGAT 
AACTTGAATC CTGATGTGCG ACTCATTCTT GAAGAGATCA CCAGGTTTTT GAATATGGAA 
AAACTTCTTC CAAGTTATAG ACATACCATC ACTGTTAGTT GTTTGAGAGC CATACGGGTG 
CTTCAGAAGA ATGGACATGT GCCAAGCGAT CCAGCTCTTT TTAAGTCTTA TGCTGAATAT 
GGCCATTTTG TGGACATTAG GATAGCAGCT TTGGAAGCAG TTGTTGATTA TACTAAAGTG 
GACAGGAGTT ATGAAGAACT ACAGTGGCTA CTTAATATGA TTCAGACTGA TCCTGTACCC 
TATGTAAGGC ATAAGATTTT AAACATGTTG ACTAAGAATC CACCTTTTAC TAAGAATATG 
GAATCTCCTT TATGCAATGA AGCCCTGGTA GATCAGCTTT GGAAACTTAT GAACTCTGGT 
ACTTCACATG ACTGGAGGTT ACGGTGTGGT GCTGTGGACT TGTACTTCAC ACTTTTTGGC 
CTCAGTAGAC CTTCCTGCTT ACCTTTGCCA GAGCTTGGGT TGGTTCTTAA TTTAAAGGAG 
AAAAAAGCTG TGTTGAATCC TACCATAATT CCAGAGGCAG TAGCAGGCAA CCAGGAAGTT 
GCGAGTAATC CAAGCAGTCA CCCACAGCTA GTTGGATTTC AGAACACATT CTCAAATTCT 
CAAGATGAGG AGGAAATTGA TATGGATACT GTTCATGATA GCCAGGCTTT CATTTCTCAT 
CATTTGAACA TGCTGGAAAG GCCATCAACT CCAGGGCTGT CTAAATATCG GCCAGCCAGT 
TCCCGGTCGG CTTTAGTGCC CCAGCACTCA TCAGGCTGCG ATGGCACACC CACCACAAAA 
CCCCCGTGGA GTATGGAGCT TGCACGTAAA GGAACAGGGA AAGAACAGTC ACCCTTGGAG 
ATGAGTATGC ATCCAGCAGT GGCACCTCCA CTCTCAGTGT TTTCTAAGGA GTCTACCTCC 
TCCAAACACA GTGACCACCA TCACCACCAT CACCACGAGC ACAAGAAAAA GAAGAAGAAG 
CACAAGCATA AGCACAAGCA CAAGCATAAG CATGACAGCA AAGAAAAGGA CAAGGAGCCT 
TTTACTTACC ACAGCCCCGC CAGTGGCAGG TCTATTCGTT CCCCCTCCCT TTCAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004373031.1
CDS	1..3600
Translation	MPPTGLEPARMNRKKGDKGFESPRPYKLTHQVVCINNINFQRKSVVGFVELTIFPTVANLNRIKLNSKQCRIYRVRVNDLEAAFIYNDPTLEVCHNESKQRNLNYFSNAYAAAVSAVDPDAGNGELCIKVPSELWKHVDELKVLKIHINFSLDQPKGGLHFVVPSVEGSMAERGAHVFSCGYQNSTRFWFPCVDSYSELCTWKLEFTVDAAMVAVSNGDLVETVYTHDMRKKTFHYMLTIPTAASNISLAIGPFEILVDPYMHEVTHFCLPQLLPLLKHTTSYLHEVFEFYEEILTCRYPYSCFKTVFIDEAYVEVAAYASMSIFSTNLLHSAMIIDETPLTRRCLAQSLAQQFFGCFISRMSWSDEWVLKGISGYIYGLWMKKTFGVNEYRHWIKEELDKIVAYELKTGGVLLHPIFGGGKEKDNPASHLHFSIKHPHTLSWEYYTMFQCKAHLVMRLIENRISMEFMLQVFNKLLSLASTASSQKFQSHMWSQMLVSTSGFLKSISNVSGKDIQPLIKQWVDQSGVVKFYGSFAFNRKRNVLELEIKQDYTSPGTQKYVGPLKVTVQELDGSFNHTLQIEENSLKHDIPCHSKSRRNKKKKIPLMNGEEVDMDLSAMDADSPLLWIRIDPDMSVLRKVEFEQADFMWQYQLRYERDVVAQQESILALEKFPTPASRLALTDILDQEQCFYRVRMSACFCLAKIANSMVSTWTGPPAMKSLFTRMFCCKTCPNIVKTNNFLSFQSYFLQKTMPVAMALLRDVHNLCPKEVLTFILDLIKYNDNRKNKFSDNYYRAEMIDALANSVTPAVSVNNEVRTLDNLNPDVRLILEEITRFLNMEKLLPSYRHTITVSCLRAIRVLQKNGHVPSDPALFKSYAEYGHFVDIRIAALEAVVDYTKVDRSYEELQWLLNMIQTDPVPYVRHKILNMLTKNPPFTKNMESPLCNEALVDQLWKLMNSGTSHDWRLRCGAVDLYFTLFGLSRPSCLPLPELGLVLNLKEKKAVLNPTIIPEAVAGNQEVASNPSSHPQLVGFQNTFSNSQDEEEIDMDTVHDSQAFISHHLNMLERPSTPGLSKYRPASSRSALVPQHSSGCDGTPTTKPPWSMELARKGTGKEQSPLEMSMHPAVAPPLSVFSKESTSSKHSDHHHHHHHEHKKKKKKHKHKHKHKHKHDSKEKDKEPFTYHSPASGRSIRSPSLSD

Target ORF information:

RefSeq Version	XM_004372974.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa (LOC101342577), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004372974.2

ORF Insert Sequence:

ATGCCGCCGA CTGGTTTAGA GCCCGCCAGA ATGAACAGGA AGAAAGGAGA CAAGGGGTTT 
GAAAGCCCAA GGCCATATAA ATTAACCCAT CAGGTGGTCT GCATCAACAA CATAAACTTC 
CAGAGAAAAT CTGTTGTGGG ATTTGTAGAA CTGACTATAT TTCCCACAGT TGCAAACTTG 
AATAGAATCA AGTTGAACAG CAAACAGTGC AGAATATACC GAGTAAGGGT CAACGACTTA 
GAGGCTGCCT TTATTTATAA TGATCCAACC TTGGAAGTTT GTCACAATGA ATCGAAACAG 
AGAAATCTCA ATTATTTTTC CAATGCTTAT GCAGCTGCAG TTAGTGCTGT GGATCCTGAT 
GCGGGAAATG GAGAACTTTG CATTAAGGTT CCCTCAGAGC TTTGGAAACA TGTTGATGAG 
TTAAAGGTCT TGAAGATACA CATCAATTTT TCTTTGGATC AGCCAAAAGG AGGTCTTCAT 
TTTGTGGTAC CCAGTGTGGA GGGAAGCATG GCTGAGAGAG GTGCTCATGT TTTCTCTTGT 
GGGTATCAAA ATTCCACAAG ATTTTGGTTC CCATGTGTTG ATTCATACTC TGAATTATGT 
ACATGGAAAT TAGAATTCAC AGTAGATGCT GCAATGGTGG CTGTTTCCAA TGGAGATTTG 
GTGGAGACAG TGTATACCCA TGATATGAGG AAGAAGACCT TCCATTACAT GCTTACCATT 
CCAACAGCAG CGTCAAATAT CTCCTTGGCC ATTGGACCTT TTGAAATACT TGTAGATCCA 
TATATGCATG AGGTGACTCA TTTTTGTTTA CCCCAACTTC TTCCATTGCT TAAACATACT 
ACGTCATATC TTCATGAAGT CTTTGAATTT TATGAGGAAA TTCTTACATG TCGATACCCA 
TACTCCTGTT TTAAAACTGT GTTCATCGAT GAGGCTTATG TTGAAGTGGC TGCTTATGCT 
TCCATGAGCA TTTTTAGCAC AAATCTTTTA CACAGTGCCA TGATTATAGA TGAGACACCT 
TTGACTAGAA GGTGTTTAGC CCAGTCCTTG GCCCAGCAGT TTTTTGGATG TTTCATATCC 
AGAATGTCTT GGTCTGATGA ATGGGTGCTG AAGGGAATTT CAGGCTATAT TTATGGACTT 
TGGATGAAAA AAACTTTTGG TGTTAATGAG TACCGCCACT GGATTAAGGA GGAACTAGAC 
AAAATAGTGG CGTATGAATT GAAGACTGGT GGAGTTTTAT TGCATCCCAT TTTTGGTGGA 
GGGAAAGAGA AGGATAATCC AGCATCCCAC CTACACTTTT CAATAAAGCA TCCACATACA 
CTCTCCTGGG AATATTACAC TATGTTCCAG TGTAAAGCTC ACCTTGTGAT GAGATTGATT 
GAAAATAGGA TCAGTATGGA ATTTATGCTG CAAGTTTTCA ATAAACTGCT AAGTCTGGCT 
AGTACTGCTT CATCTCAGAA GTTCCAGTCA CATATGTGGA GTCAGATGTT GGTTTCCACA 
TCTGGGTTTT TGAAGTCCAT CTCCAATGTC TCTGGCAAAG ACATCCAGCC TTTAATAAAG 
CAGTGGGTAG ACCAGAGTGG AGTAGTAAAA TTTTATGGAA GTTTTGCATT TAATAGAAAA 
CGAAATGTCT TGGAATTGGA AATAAAACAA GATTATACAT CTCCTGGAAC TCAGAAATAT 
GTGGGACCAC TTAAAGTAAC AGTGCAGGAG TTAGATGGAT CCTTCAATCA TACATTGCAA 
ATTGAAGAAA ACAGCCTTAA ACATGATATA CCCTGCCATT CCAAAAGCAG ACGAAATAAG 
AAGAAAAAGA TTCCGCTGAT GAATGGAGAA GAAGTTGACA TGGATCTTTC TGCAATGGAT 
GCTGATTCCC CGTTACTGTG GATAAGGATA GACCCAGATA TGTCAGTACT GAGGAAGGTA 
GAATTTGAAC AAGCAGATTT TATGTGGCAG TACCAACTCC GCTATGAGAG GGATGTTGTT 
GCACAGCAGG AATCCATCTT AGCCTTGGAG AAGTTCCCTA CTCCAGCCTC TCGGCTTGCA 
CTCACTGACA TATTAGACCA AGAACAGTGT TTCTACCGAG TAAGAATGTC AGCTTGCTTC 
TGTCTTGCGA AGATTGCAAA TTCCATGGTG AGCACATGGA CAGGACCACC AGCTATGAAG 
TCACTCTTCA CTAGAATGTT TTGTTGTAAA ACTTGTCCAA ACATTGTGAA AACAAACAAC 
TTTTTGAGCT TTCAAAGTTA TTTTCTACAG AAGACTATGC CAGTTGCAAT GGCTTTGTTG 
AGAGATGTTC ATAACCTCTG TCCCAAAGAA GTCTTAACGT TTATTTTAGA CTTAATCAAG 
TATAATGATA ACAGAAAAAA TAAGTTTTCA GATAACTATT ATCGTGCAGA AATGATTGAT 
GCCCTTGCCA ACTCTGTTAC ACCTGCAGTC AGTGTGAATA ATGAAGTTAG AACTTTGGAT 
AACTTGAATC CTGATGTGCG ACTCATTCTT GAAGAGATCA CCAGGTTTTT GAATATGGAA 
AAACTTCTTC CAAGTTATAG ACATACCATC ACTGTTAGTT GTTTGAGAGC CATACGGGTG 
CTTCAGAAGA ATGGACATGT GCCAAGCGAT CCAGCTCTTT TTAAGTCTTA TGCTGAATAT 
GGCCATTTTG TGGACATTAG GATAGCAGCT TTGGAAGCAG TTGTTGATTA TACTAAAGTG 
GACAGGAGTT ATGAAGAACT ACAGTGGCTA CTTAATATGA TTCAGACTGA TCCTGTACCC 
TATGTAAGGC ATAAGATTTT AAACATGTTG ACTAAGAATC CACCTTTTAC TAAGAATATG 
GAATCTCCTT TATGCAATGA AGCCCTGGTA GATCAGCTTT GGAAACTTAT GAACTCTGGT 
ACTTCACATG ACTGGAGGTT ACGGTGTGGT GCTGTGGACT TGTACTTCAC ACTTTTTGGC 
CTCAGTAGAC CTTCCTGCTT ACCTTTGCCA GAGCTTGGGT TGGTTCTTAA TTTAAAGGAG 
AAAAAAGCTG TGTTGAATCC TACCATAATT CCAGAGGCAG TAGCAGGCAA CCAGGAAGTT 
GCGAGTAATC CAAGCAGTCA CCCACAGCTA GTTGGATTTC AGAACACATT CTCAAATTCT 
CAAGATGAGG AGGAAATTGA TATGGATACT GTTCATGATA GCCAGGCTTT CATTTCTCAT 
CATTTGAACA TGCTGGAAAG GCCATCAACT CCAGGGCTGT CTAAATATCG GCCAGCCAGT 
TCCCGGTCGG CTTTAGTGCC CCAGCACTCA TCAGGCTGCG ATGGCACACC CACCACAAAA 
CCCCCGTGGA GTATGGAGCT TGCACGTAAA GGAACAGGGA AAGAACAGTC ACCCTTGGAG 
ATGAGTATGC ATCCAGCAGT GGCACCTCCA CTCTCAGTGT TTTCTAAGGA GTCTACCTCC 
TCCAAACACA GTGACCACCA TCACCACCAT CACCACGAGC ACAAGAAAAA GAAGAAGAAG 
CACAAGCATA AGCACAAGCA CAAGCATAAG CATGACAGCA AAGAAAAGGA CAAGGAGCCT 
TTTACTTACC ACAGCCCCGC CAGTGGCAGG TCTATTCGTT CCCCCTCCCT TTCAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101342577 ( XM_012554539.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_012409993.1 Trichechus manatus latirostris TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa (LOC101342577), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTr169399
Clone ID Related Accession (Same CDS sequence)	XM_012554539.1
Accession Version	XM_012554539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	transcription initiation factor TFIID subunit 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443958.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCCGA CTGGTTTAGA GCCCGCCAGA ATGAACAGGA AGAAAGGAGA CAAGGGGTTT 
GAAAGCCCAA GGCCATATAA ATTAACCCAT CAGGTGGTCT GCATCAACAA CATAAACTTC 
CAGAGAAAAT CTGTTGTGGG ATTTGTAGAA CTGACTATAT TTCCCACAGT TGCAAACTTG 
AATAGAATCA AGTTGAACAG CAAACAGTGC AGAATATACC GAGTAAGGGT CAACGACTTA 
GAGGCTGCCT TTATTTATAA TGATCCAACC TTGGAAGTTT GTCACAATGA ATCGAAACAG 
AGAAATCTCA ATTATTTTTC CAATGCTTAT GCAGCTGCAG TTAGTGCTGT GGATCCTGAT 
GCGGGAAATG GAGAACTTTG CATTAAGGTT CCCTCAGAGC TTTGGAAACA TGTTGATGAG 
TTAAAGGTCT TGAAGATACA CATCAATTTT TCTTTGGATC AGCCAAAAGG AGGTCTTCAT 
TTTGTGGTAC CCAGTGTGGA GGGAAGCATG GCTGAGAGAG GTGCTCATGT TTTCTCTTGT 
GGGTATCAAA ATTCCACAAG ATTTTGGTTC CCATGTGTTG ATTCATACTC TGAATTATGT 
ACATGGAAAT TAGAATTCAC AGTAGATGCT GCAATGGTGG CTGTTTCCAA TGGAGATTTG 
GTGGAGACAG TGTATACCCA TGATATGAGG AAGAAGACCT TCCATTACAT GCTTACCATT 
CCAACAGCAG CGTCAAATAT CTCCTTGGCC ATTGGACCTT TTGAAATACT TGTAGATCCA 
TATATGCATG AGGTGACTCA TTTTTGTTTA CCCCAACTTC TTCCATTGCT TAAACATACT 
ACGTCATATC TTCATGAAGT CTTTGAATTT TATGAGGAAA TTCTTACATG TCGATACCCA 
TACTCCTGTT TTAAAACTGT GTTCATCGAT GAGGCTTATG TTGAAGTGGC TGCTTATGCT 
TCCATGAGCA TTTTTAGCAC AAATCTTTTA CACAGTGCCA TGATTATAGA TGAGACACCT 
TTGACTAGAA GGTGTTTAGC CCAGTCCTTG GCCCAGCAGT TTTTTGGATG TTTCATATCC 
AGAATGTCTT GGTCTGATGA ATGGGTGCTG AAGGGAATTT CAGGCTATAT TTATGGACTT 
TGGATGAAAA AAACTTTTGG TGTTAATGAG TACCGCCACT GGATTAAGGA GGAACTAGAC 
AAAATAGTGG CGTATGAATT GAAGACTGGT GGAGTTTTAT TGCATCCCAT TTTTGGTGGA 
GGGAAAGAGA AGGATAATCC AGCATCCCAC CTACACTTTT CAATAAAGCA TCCACATACA 
CTCTCCTGGG AATATTACAC TATGTTCCAG TGTAAAGCTC ACCTTGTGAT GAGATTGATT 
GAAAATAGGA TCAGTATGGA ATTTATGCTG CAAGTTTTCA ATAAACTGCT AAGTCTGGCT 
AGTACTGCTT CATCTCAGAA GTTCCAGTCA CATATGTGGA GTCAGATGTT GGTTTCCACA 
TCTGGGTTTT TGAAGTCCAT CTCCAATGTC TCTGGCAAAG ACATCCAGCC TTTAATAAAG 
CAGTGGGTAG ACCAGAGTGG AGTAGTAAAA TTTTATGGAA GTTTTGCATT TAATAGAAAA 
CGAAATGTCT TGGAATTGGA AATAAAACAA GATTATACAT CTCCTGGAAC TCAGAAATAT 
GTGGGACCAC TTAAAGTAAC AGTGCAGGAG TTAGATGGAT CCTTCAATCA TACATTGCAA 
ATTGAAGAAA ACAGCCTTAA ACATGATATA CCCTGCCATT CCAAAAGCAG ACGGTACAAA 
AAGATTCCGC TGATGAATGG AGAAGAAGTT GACATGGATC TTTCTGCAAT GGATGCTGAT 
TCCCCGTTAC TGTGGATAAG GATAGACCCA GATATGTCAG TACTGAGGAA GGTAGAATTT 
GAACAAGCAG ATTTTATGTG GCAGTACCAA CTCCGCTATG AGAGGGATGT TGTTGCACAG 
CAGGAATCCA TCTTAGCCTT GGAGAAGTTC CCTACTCCAG CCTCTCGGCT TGCACTCACT 
GACATATTAG ACCAAGAACA GTGTTTCTAC CGAGTAAGAA TGTCAGCTTG CTTCTGTCTT 
GCGAAGATTG CAAATTCCAT GGTGAGCACA TGGACAGGAC CACCAGCTAT GAAGTCACTC 
TTCACTAGAA TGTTTTGTTG TAAAACTTGT CCAAACATTG TGAAAACAAA CAACTTTTTG 
AGCTTTCAAA GTTATTTTCT ACAGAAGACT ATGCCAGTTG CAATGGCTTT GTTGAGAGAT 
GTTCATAACC TCTGTCCCAA AGAAGTCTTA ACGTTTATTT TAGACTTAAT CAAGTATAAT 
GATAACAGAA AAAATAAGTT TTCAGATAAC TATTATCGTG CAGAAATGAT TGATGCCCTT 
GCCAACTCTG TTACACCTGC AGTCAGTGTG AATAATGAAG TTAGAACTTT GGATAACTTG 
AATCCTGATG TGCGACTCAT TCTTGAAGAG ATCACCAGGT TTTTGAATAT GGAAAAACTT 
CTTCCAAGTT ATAGACATAC CATCACTGTT AGTTGTTTGA GAGCCATACG GGTGCTTCAG 
AAGAATGGAC ATGTGCCAAG CGATCCAGCT CTTTTTAAGT CTTATGCTGA ATATGGCCAT 
TTTGTGGACA TTAGGATAGC AGCTTTGGAA GCAGTTGTTG ATTATACTAA AGTGGACAGG 
AGTTATGAAG AACTACAGTG GCTACTTAAT ATGATTCAGA CTGATCCTGT ACCCTATGTA 
AGGCATAAGA TTTTAAACAT GTTGACTAAG AATCCACCTT TTACTAAGAA TATGGAATCT 
CCTTTATGCA ATGAAGCCCT GGTAGATCAG CTTTGGAAAC TTATGAACTC TGGTACTTCA 
CATGACTGGA GGTTACGGTG TGGTGCTGTG GACTTGTACT TCACACTTTT TGGCCTCAGT 
AGACCTTCCT GCTTACCTTT GCCAGAGCTT GGGTTGGTTC TTAATTTAAA GGAGAAAAAA 
GCTGTGTTGA ATCCTACCAT AATTCCAGAG GCAGTAGCAG GCAACCAGGA AGTTGCGAGT 
AATCCAAGCA GTCACCCACA GCTAGTTGGA TTTCAGAACA CATTCTCAAA TTCTCAAGAT 
GAGGAGGAAA TTGATATGGA TACTGTTCAT GATAGCCAGG CTTTCATTTC TCATCATTTG 
AACATGCTGG AAAGGCCATC AACTCCAGGG CTGTCTAAAT ATCGGCCAGC CAGTTCCCGG 
TCGGCTTTAG TGCCCCAGCA CTCATCAGGC TGCGATGGCA CACCCACCAC AAAACCCCCG 
TGGAGTATGG AGCTTGCACG TAAAGGAACA GGGAAAGAAC AGTCACCCTT GGAGATGAGT 
ATGCATCCAG CAGTGGCACC TCCACTCTCA GTGTTTTCTA AGGAGTCTAC CTCCTCCAAA 
CACAGTGACC ACCATCACCA CCATCACCAC GAGCACAAGA AAAAGAAGAA GAAGCACAAG 
CATAAGCACA AGCACAAGCA TAAGCATGAC AGCAAAGAAA AGGACAAGGA GCCTTTTACT 
TACCACAGCC CCGCCAGTGG CAGGTCTATT CGTTCCCCCT CCCTTTCAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012409993.1
CDS	304..3897
Translation	MPPTGLEPARMNRKKGDKGFESPRPYKLTHQVVCINNINFQRKSVVGFVELTIFPTVANLNRIKLNSKQCRIYRVRVNDLEAAFIYNDPTLEVCHNESKQRNLNYFSNAYAAAVSAVDPDAGNGELCIKVPSELWKHVDELKVLKIHINFSLDQPKGGLHFVVPSVEGSMAERGAHVFSCGYQNSTRFWFPCVDSYSELCTWKLEFTVDAAMVAVSNGDLVETVYTHDMRKKTFHYMLTIPTAASNISLAIGPFEILVDPYMHEVTHFCLPQLLPLLKHTTSYLHEVFEFYEEILTCRYPYSCFKTVFIDEAYVEVAAYASMSIFSTNLLHSAMIIDETPLTRRCLAQSLAQQFFGCFISRMSWSDEWVLKGISGYIYGLWMKKTFGVNEYRHWIKEELDKIVAYELKTGGVLLHPIFGGGKEKDNPASHLHFSIKHPHTLSWEYYTMFQCKAHLVMRLIENRISMEFMLQVFNKLLSLASTASSQKFQSHMWSQMLVSTSGFLKSISNVSGKDIQPLIKQWVDQSGVVKFYGSFAFNRKRNVLELEIKQDYTSPGTQKYVGPLKVTVQELDGSFNHTLQIEENSLKHDIPCHSKSRRYKKIPLMNGEEVDMDLSAMDADSPLLWIRIDPDMSVLRKVEFEQADFMWQYQLRYERDVVAQQESILALEKFPTPASRLALTDILDQEQCFYRVRMSACFCLAKIANSMVSTWTGPPAMKSLFTRMFCCKTCPNIVKTNNFLSFQSYFLQKTMPVAMALLRDVHNLCPKEVLTFILDLIKYNDNRKNKFSDNYYRAEMIDALANSVTPAVSVNNEVRTLDNLNPDVRLILEEITRFLNMEKLLPSYRHTITVSCLRAIRVLQKNGHVPSDPALFKSYAEYGHFVDIRIAALEAVVDYTKVDRSYEELQWLLNMIQTDPVPYVRHKILNMLTKNPPFTKNMESPLCNEALVDQLWKLMNSGTSHDWRLRCGAVDLYFTLFGLSRPSCLPLPELGLVLNLKEKKAVLNPTIIPEAVAGNQEVASNPSSHPQLVGFQNTFSNSQDEEEIDMDTVHDSQAFISHHLNMLERPSTPGLSKYRPASSRSALVPQHSSGCDGTPTTKPPWSMELARKGTGKEQSPLEMSMHPAVAPPLSVFSKESTSSKHSDHHHHHHHEHKKKKKKHKHKHKHKHKHDSKEKDKEPFTYHSPASGRSIRSPSLSD

Target ORF information:

RefSeq Version	XM_012554539.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa (LOC101342577), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012554539.1

ORF Insert Sequence:

ATGCCGCCGA CTGGTTTAGA GCCCGCCAGA ATGAACAGGA AGAAAGGAGA CAAGGGGTTT 
GAAAGCCCAA GGCCATATAA ATTAACCCAT CAGGTGGTCT GCATCAACAA CATAAACTTC 
CAGAGAAAAT CTGTTGTGGG ATTTGTAGAA CTGACTATAT TTCCCACAGT TGCAAACTTG 
AATAGAATCA AGTTGAACAG CAAACAGTGC AGAATATACC GAGTAAGGGT CAACGACTTA 
GAGGCTGCCT TTATTTATAA TGATCCAACC TTGGAAGTTT GTCACAATGA ATCGAAACAG 
AGAAATCTCA ATTATTTTTC CAATGCTTAT GCAGCTGCAG TTAGTGCTGT GGATCCTGAT 
GCGGGAAATG GAGAACTTTG CATTAAGGTT CCCTCAGAGC TTTGGAAACA TGTTGATGAG 
TTAAAGGTCT TGAAGATACA CATCAATTTT TCTTTGGATC AGCCAAAAGG AGGTCTTCAT 
TTTGTGGTAC CCAGTGTGGA GGGAAGCATG GCTGAGAGAG GTGCTCATGT TTTCTCTTGT 
GGGTATCAAA ATTCCACAAG ATTTTGGTTC CCATGTGTTG ATTCATACTC TGAATTATGT 
ACATGGAAAT TAGAATTCAC AGTAGATGCT GCAATGGTGG CTGTTTCCAA TGGAGATTTG 
GTGGAGACAG TGTATACCCA TGATATGAGG AAGAAGACCT TCCATTACAT GCTTACCATT 
CCAACAGCAG CGTCAAATAT CTCCTTGGCC ATTGGACCTT TTGAAATACT TGTAGATCCA 
TATATGCATG AGGTGACTCA TTTTTGTTTA CCCCAACTTC TTCCATTGCT TAAACATACT 
ACGTCATATC TTCATGAAGT CTTTGAATTT TATGAGGAAA TTCTTACATG TCGATACCCA 
TACTCCTGTT TTAAAACTGT GTTCATCGAT GAGGCTTATG TTGAAGTGGC TGCTTATGCT 
TCCATGAGCA TTTTTAGCAC AAATCTTTTA CACAGTGCCA TGATTATAGA TGAGACACCT 
TTGACTAGAA GGTGTTTAGC CCAGTCCTTG GCCCAGCAGT TTTTTGGATG TTTCATATCC 
AGAATGTCTT GGTCTGATGA ATGGGTGCTG AAGGGAATTT CAGGCTATAT TTATGGACTT 
TGGATGAAAA AAACTTTTGG TGTTAATGAG TACCGCCACT GGATTAAGGA GGAACTAGAC 
AAAATAGTGG CGTATGAATT GAAGACTGGT GGAGTTTTAT TGCATCCCAT TTTTGGTGGA 
GGGAAAGAGA AGGATAATCC AGCATCCCAC CTACACTTTT CAATAAAGCA TCCACATACA 
CTCTCCTGGG AATATTACAC TATGTTCCAG TGTAAAGCTC ACCTTGTGAT GAGATTGATT 
GAAAATAGGA TCAGTATGGA ATTTATGCTG CAAGTTTTCA ATAAACTGCT AAGTCTGGCT 
AGTACTGCTT CATCTCAGAA GTTCCAGTCA CATATGTGGA GTCAGATGTT GGTTTCCACA 
TCTGGGTTTT TGAAGTCCAT CTCCAATGTC TCTGGCAAAG ACATCCAGCC TTTAATAAAG 
CAGTGGGTAG ACCAGAGTGG AGTAGTAAAA TTTTATGGAA GTTTTGCATT TAATAGAAAA 
CGAAATGTCT TGGAATTGGA AATAAAACAA GATTATACAT CTCCTGGAAC TCAGAAATAT 
GTGGGACCAC TTAAAGTAAC AGTGCAGGAG TTAGATGGAT CCTTCAATCA TACATTGCAA 
ATTGAAGAAA ACAGCCTTAA ACATGATATA CCCTGCCATT CCAAAAGCAG ACGGTACAAA 
AAGATTCCGC TGATGAATGG AGAAGAAGTT GACATGGATC TTTCTGCAAT GGATGCTGAT 
TCCCCGTTAC TGTGGATAAG GATAGACCCA GATATGTCAG TACTGAGGAA GGTAGAATTT 
GAACAAGCAG ATTTTATGTG GCAGTACCAA CTCCGCTATG AGAGGGATGT TGTTGCACAG 
CAGGAATCCA TCTTAGCCTT GGAGAAGTTC CCTACTCCAG CCTCTCGGCT TGCACTCACT 
GACATATTAG ACCAAGAACA GTGTTTCTAC CGAGTAAGAA TGTCAGCTTG CTTCTGTCTT 
GCGAAGATTG CAAATTCCAT GGTGAGCACA TGGACAGGAC CACCAGCTAT GAAGTCACTC 
TTCACTAGAA TGTTTTGTTG TAAAACTTGT CCAAACATTG TGAAAACAAA CAACTTTTTG 
AGCTTTCAAA GTTATTTTCT ACAGAAGACT ATGCCAGTTG CAATGGCTTT GTTGAGAGAT 
GTTCATAACC TCTGTCCCAA AGAAGTCTTA ACGTTTATTT TAGACTTAAT CAAGTATAAT 
GATAACAGAA AAAATAAGTT TTCAGATAAC TATTATCGTG CAGAAATGAT TGATGCCCTT 
GCCAACTCTG TTACACCTGC AGTCAGTGTG AATAATGAAG TTAGAACTTT GGATAACTTG 
AATCCTGATG TGCGACTCAT TCTTGAAGAG ATCACCAGGT TTTTGAATAT GGAAAAACTT 
CTTCCAAGTT ATAGACATAC CATCACTGTT AGTTGTTTGA GAGCCATACG GGTGCTTCAG 
AAGAATGGAC ATGTGCCAAG CGATCCAGCT CTTTTTAAGT CTTATGCTGA ATATGGCCAT 
TTTGTGGACA TTAGGATAGC AGCTTTGGAA GCAGTTGTTG ATTATACTAA AGTGGACAGG 
AGTTATGAAG AACTACAGTG GCTACTTAAT ATGATTCAGA CTGATCCTGT ACCCTATGTA 
AGGCATAAGA TTTTAAACAT GTTGACTAAG AATCCACCTT TTACTAAGAA TATGGAATCT 
CCTTTATGCA ATGAAGCCCT GGTAGATCAG CTTTGGAAAC TTATGAACTC TGGTACTTCA 
CATGACTGGA GGTTACGGTG TGGTGCTGTG GACTTGTACT TCACACTTTT TGGCCTCAGT 
AGACCTTCCT GCTTACCTTT GCCAGAGCTT GGGTTGGTTC TTAATTTAAA GGAGAAAAAA 
GCTGTGTTGA ATCCTACCAT AATTCCAGAG GCAGTAGCAG GCAACCAGGA AGTTGCGAGT 
AATCCAAGCA GTCACCCACA GCTAGTTGGA TTTCAGAACA CATTCTCAAA TTCTCAAGAT 
GAGGAGGAAA TTGATATGGA TACTGTTCAT GATAGCCAGG CTTTCATTTC TCATCATTTG 
AACATGCTGG AAAGGCCATC AACTCCAGGG CTGTCTAAAT ATCGGCCAGC CAGTTCCCGG 
TCGGCTTTAG TGCCCCAGCA CTCATCAGGC TGCGATGGCA CACCCACCAC AAAACCCCCG 
TGGAGTATGG AGCTTGCACG TAAAGGAACA GGGAAAGAAC AGTCACCCTT GGAGATGAGT 
ATGCATCCAG CAGTGGCACC TCCACTCTCA GTGTTTTCTA AGGAGTCTAC CTCCTCCAAA 
CACAGTGACC ACCATCACCA CCATCACCAC GAGCACAAGA AAAAGAAGAA GAAGCACAAG 
CATAAGCACA AGCACAAGCA TAAGCATGAC AGCAAAGAAA AGGACAAGGA GCCTTTTACT 
TACCACAGCC CCGCCAGTGG CAGGTCTATT CGTTCCCCCT CCCTTTCAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC101342577 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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電話

FAX

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