LOC101340282 cDNA ORF clone, Trichechus manatus latirostris(Florida manatee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101340282
Entrez Gene ID	101340282
Full Name	focadhesin
Synonyms	FOCAD
Gene Type	protein-coding
Organism	Trichechus manatus latirostris(Florida manatee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012554721.2	XP_012410175.2	focadhesin
XM_012554721.1	XP_012410175.1	focadhesin

Pathways from BioSystems
Gene Ontology

The following LOC101340282 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101340282 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTr170006	XM_012554721.1 Latest version!	Trichechus manatus latirostris focadhesin (LOC101340282), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OTr217144	XM_012554721.2 Latest version!	Trichechus manatus latirostris focadhesin (LOC101340282), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101340282 ( XM_012554721.1 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_012410175.1 Trichechus manatus latirostris focadhesin (LOC101340282), mRNA.

Price & Availability↑

CloneID	OTr170006
Clone ID Related Accession (Same CDS sequence)	XM_012554721.1
Accession Version	XM_012554721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3720bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Trichechus manatus latirostris(Florida manatee)
Product	focadhesin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443961.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Trichechus manatus latirostris Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATGT CTGATGTGCC TTCTCTGTCT GTGGGCAGGG AGGTCCAGTG GGAGAAACTA 
ATCGCGAAAG CAGCATCGAT CAGAGATATA TGTAAGCAAA GACCATATCA GCATGGTGCT 
GATATGTTGG CAGCTATTTC TCAGGTGTTA AACGAATGCA CTAAATCTGA TCAAGCTACT 
CCAGCAGCCT TGGTGTTACA AGGTCTTCAT GCGCTCTGTC AAGCTGAGGT TGTTTGTATT 
CGCTCTACTT GGAATGCTCT CTCCCCAAAA CTGAGTTGTG ACACGAGACC TCTCATTTTA 
AAGACCCTCA GTGAACTCTT TTCTCTGGTT CCTTCCTTAA CAGTCAATAC AGCTGAATAT 
GAGAATTTTA AAGTTCAAGT CCTCAGCTTC CTCTGGACTC AAACTCAAAA CAAGGACCCA 
GTTGTAGCAA ATGCTGCATA TAAATCTCTG TCACAGTTTA GCGCAGGGGA GCACACCATT 
CTTCATCTGC CTGAAAAGAT AAGACCAGAA ATTCCTGTTT CTGATGAGCT AGATGAAGAA 
GAAGATGATG AGGGTGATGA AAAGGAAGTG GACCTTTCAA TTCCTGGTTT TTGCTATCTC 
AAACTGTTGT CACTTACTGC TCCTTCAGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAAC AAGAAATGGT GAATATGCCC CGTGGGGTGT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGTT CAGATCAAGG AAAGACTATT GCAGGAATCC CCAATTTTAT GTTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CCGGTGGCAT GTTATTTTGT 
TACGATATTT CAATGTATCA GAGTAAAGAT GGCAAACCAT TGAATCGATT GATGGCCAGT 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTGC ATGAGGTTCA CATTCAGCTC 
TTGGAGTGGC ACCGTGCAGT ATTTCTCCCA CAGGCCTGGC TTGCATATAT GAATCGAGCA 
TATCATGCCA TTTTACAGGG AAGAATAGCA GAGTTGGAGT TACAGTTAAA ACACGGAAAA 
GAAGGACCTG AGGAGGTGCA GTACAAGAAA AGCACAGCCT GGCTCTGGGT CAGAGACATG 
TTGACTGATG AGATCACCAA GGCAGCTGCA AAGGACAGCC CAGTGGTCAA AGGGAACGCA 
CTGTTAGCCT TAAGTAGCCT TGCTGTTGTC GTATCCAAAC ATGAAGCCAG CCTCTCCTCA 
GACTCGGATG AGGCCCTGGA GATTCAACCT AACTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGGTGCTTG ATACCCTCCT GGTAATTGTG GATAGTCATT ACCAACCCCG AGGACAACTC 
TTCTCCTGGT TTTATTATAA ATCCTATTCC GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTC GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAC 
GAGTCTCAGG CCGTGCAAAT CCACATGGGC CTTGCTTTGG GGATGTTTCT CTCTCGTTTG 
TGTGAGGAGA AACTCAGTGA TATATCTGGC CAACAGATGA ACCTTCTTCT CATGAAGTCT 
TTGAATGCTC TGGAAAATTG CTGCTTCGAC ACCAGTCTTG AATACAACAC GGGCTGTATA 
CTGGGAGTTG GACTTGTTCT GTGTCTGATG AGCCACAGCA GCCACACGGA ATCCCGGGTC 
CACGTGGCAG CATCGCTTCG AAAGCTCTCT ACATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTATACC CTTAGCTGTG TATGTACGTC AGCGTTCAGT 
GCTGGAATTA TCGAGGCAGC AGAGGCTGAA GATATTATGA ATAAACTTCG GCTGTTAGTA 
GAGAACAACC AGCAGACTTC AGGTTTTGCC CTGGCATTAG GAAATATAGT TCATGGTTTG 
TCTGTGTGTG GACACGGAAA AGCTGAAGAC TTGGGCAACA AACTGCTTCC TGCCTGGATC 
AGAATTGTTC TATCAGAGGG CACTCCCACG ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCTTGG TAGGCTCTGA AGGGGATGTA ATACAGCTGA AGTCAGAAGC CATCCAGACC 
TCTGAGTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATCAGTGTC 
TCTGGGATGA TCGGCCTTCA GTCAAATGCA ATCTGGCTTC TAGGACATCT TCATCTATCT 
ACTCTCTCCT CAAATCAAAG TAGAAGCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TGAGAGCAGC CATTGGCTTC TTCGTTACGG GAGGAAAAAA AGGTCCTGAG 
TCTGTACCTC CTTCCCTTCT TAAAGTAGTG ATGAAGCCCA TAGCAACTGT TGGAGAAAAC 
TACCAATTCC CTCCTGTGAA CTGGGCTTCA CTCCTCTCAC CACTGATGAG ACTAAACTTT 
GGTGAAGAGA TACAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCACAG 
AATGCAGCTG CTCTGCTGGG CTTGTGGACA ATGCCACCAC TAATCCATGG TCTGAGTCTG 
AATATCAAGA AATACCTCCT GGTATCTGCA CCTCTGTGGA TAAAACATGT CTCTGAGGAA 
CAGCTCCTGG CTTTTGTTGA AAACTTAATG GTGGCAGTTT TTCAAGCTGC TCCCCGCATT 
TCAAGTACTG AGTTATGTCA GAATTCCTTA CAGGGTCTAA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCCC ACCACCTCTG GAGTCTGCTC TGTGATGCTG TTGGGAAAAT TTTTGACCTT 
CTGCCAAATA AGATTCGGAG AAATGATATA GAGCTGTATA TCAGTGTAGC AAAATGCCTC 
TTGGAAATGA CAGATGACAA CATCAATCGA GTTGCCCAGA TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAATC TCTCAAGGAC GATTCCCCTT GATGCACTTG 
TCTGATGTGC TCAGTGTCGC TGTGCAGCAC CAGGAGAGGG AGACACTGGC CTGGATGATA 
CTGCACAGCT TATACCAGGC GCGGATCGTG AGCCATGCCA ACACAGGCGT GTTGAAGCGA 
ATGGAGTGGC TCTTGGAAAT GATGGGTTAT ATTAGAAGTG TTGCTTACCA GTCAACATCT 
GTTCAGAATG TGGCTCTTGA TAAGGCCCTG GACTTCTTGT TGTTGATATT TGCAGCAGCA 
GTCGTTGCGT GGGCAGACCA TGCAGCCCCT CTTCTCCTTG GCCTCAGTGC CAGCTGGTTG 
CCATGGCATC TTGGCTCAGC TGGGTCAGCA CCCAGCTTCC TTGGCAGGAG TCCCATGCAC 
AGGGTCATTC TGGAGGAGGT TCTCACTCTC CTTCCTAGTA GCATGCCTCT TCTGCTGCAG 
AAGGAGCCAT GGAAGGAGCA GACTCAGAAG TTCATTGACT GGCTATTCAA CATCATGGAA 
GCCCCCAAAG AAGCCCTTTC AGCAAAATCC AGGAATCTGT TAAAAGCCAC CCTCCTGTCC 
TTGAGAGTTC TCCCAGAGTT TAAGAAGAAA GCTGTATGGA CCAGAGCATA TGGTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012410175.1
CDS	161..3880
Translation	MAMSDVPSLSVGREVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKSDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTAEYENFKVQVLSFLWTQTQNKDPVVANAAYKSLSQFSAGEHTILHLPEKIRPEIPVSDELDEEEDDEGDEKEVDLSIPGFCYLKLLSLTAPSVLPALEEFFTSLVKQEMVNMPRGVYHSALKGGVRSDQGKTIAGIPNFMLKMYETNKQPGLKPGLAGGMLFCYDISMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLLEWHRAVFLPQAWLAYMNRAYHAILQGRIAELELQLKHGKEGPEEVQYKKSTAWLWVRDMLTDEITKAAAKDSPVVKGNALLALSSLAVVVSKHEASLSSDSDEALEIQPNFLSMKEWVSMVLDTLLVIVDSHYQPRGQLFSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDISGQQMNLLLMKSLNALENCCFDTSLEYNTGCILGVGLVLCLMSHSSHTESRVHVAASLRKLSTYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEAAEAEDIMNKLRLLVENNQQTSGFALALGNIVHGLSVCGHGKAEDLGNKLLPAWIRIVLSEGTPTMLCLAALHGMVALVGSEGDVIQLKSEAIQTSEFQGRLNEVIRTLTQVISVSGMIGLQSNAIWLLGHLHLSTLSSNQSRSSVPTDYSYLPESSFMRAAIGFFVTGGKKGPESVPPSLLKVVMKPIATVGENYQFPPVNWASLLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWTMPPLIHGLSLNIKKYLLVSAPLWIKHVSEEQLLAFVENLMVAVFQAAPRISSTELCQNSLQGLSQAMKLPSPAHHLWSLLCDAVGKIFDLLPNKIRRNDIELYISVAKCLLEMTDDNINRVAQITKSNIEKAAFVKLYLISQGRFPLMHLSDVLSVAVQHQERETLAWMILHSLYQARIVSHANTGVLKRMEWLLEMMGYIRSVAYQSTSVQNVALDKALDFLLLIFAAAVVAWADHAAPLLLGLSASWLPWHLGSAGSAPSFLGRSPMHRVILEEVLTLLPSSMPLLLQKEPWKEQTQKFIDWLFNIMEAPKEALSAKSRNLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_012554721.1
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris focadhesin (LOC101340282), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012554721.1

ORF Insert Sequence:

ATGGCCATGT CTGATGTGCC TTCTCTGTCT GTGGGCAGGG AGGTCCAGTG GGAGAAACTA 
ATCGCGAAAG CAGCATCGAT CAGAGATATA TGTAAGCAAA GACCATATCA GCATGGTGCT 
GATATGTTGG CAGCTATTTC TCAGGTGTTA AACGAATGCA CTAAATCTGA TCAAGCTACT 
CCAGCAGCCT TGGTGTTACA AGGTCTTCAT GCGCTCTGTC AAGCTGAGGT TGTTTGTATT 
CGCTCTACTT GGAATGCTCT CTCCCCAAAA CTGAGTTGTG ACACGAGACC TCTCATTTTA 
AAGACCCTCA GTGAACTCTT TTCTCTGGTT CCTTCCTTAA CAGTCAATAC AGCTGAATAT 
GAGAATTTTA AAGTTCAAGT CCTCAGCTTC CTCTGGACTC AAACTCAAAA CAAGGACCCA 
GTTGTAGCAA ATGCTGCATA TAAATCTCTG TCACAGTTTA GCGCAGGGGA GCACACCATT 
CTTCATCTGC CTGAAAAGAT AAGACCAGAA ATTCCTGTTT CTGATGAGCT AGATGAAGAA 
GAAGATGATG AGGGTGATGA AAAGGAAGTG GACCTTTCAA TTCCTGGTTT TTGCTATCTC 
AAACTGTTGT CACTTACTGC TCCTTCAGTT TTACCAGCTT TGGAGGAATT TTTTACATCA 
CTTGTGAAAC AAGAAATGGT GAATATGCCC CGTGGGGTGT ATCACTCTGC ATTAAAAGGA 
GGTGTCCGTT CAGATCAAGG AAAGACTATT GCAGGAATCC CCAATTTTAT GTTGAAAATG 
TATGAAACAA ACAAGCAACC AGGATTGAAA CCTGGCCTTG CCGGTGGCAT GTTATTTTGT 
TACGATATTT CAATGTATCA GAGTAAAGAT GGCAAACCAT TGAATCGATT GATGGCCAGT 
AGAGGGCGAA GTTTCAAGCA GACATCACTT GCTCTTGTGC ATGAGGTTCA CATTCAGCTC 
TTGGAGTGGC ACCGTGCAGT ATTTCTCCCA CAGGCCTGGC TTGCATATAT GAATCGAGCA 
TATCATGCCA TTTTACAGGG AAGAATAGCA GAGTTGGAGT TACAGTTAAA ACACGGAAAA 
GAAGGACCTG AGGAGGTGCA GTACAAGAAA AGCACAGCCT GGCTCTGGGT CAGAGACATG 
TTGACTGATG AGATCACCAA GGCAGCTGCA AAGGACAGCC CAGTGGTCAA AGGGAACGCA 
CTGTTAGCCT TAAGTAGCCT TGCTGTTGTC GTATCCAAAC ATGAAGCCAG CCTCTCCTCA 
GACTCGGATG AGGCCCTGGA GATTCAACCT AACTTCCTTT CAATGAAAGA GTGGGTTTCC 
ATGGTGCTTG ATACCCTCCT GGTAATTGTG GATAGTCATT ACCAACCCCG AGGACAACTC 
TTCTCCTGGT TTTATTATAA ATCCTATTCC GGTGAAAACA CAGCTAGTGC CATTGCCCGT 
TCTGCTGCCG CCACGGCTTT GTCTCTCCTC GTGCCAGTTT TCATTATCTC TTGCAAAGAG 
AAGGTTGAGG AAATCCTGAA CATGCTGACT GCCAGGTTAC CTGGGAAACC AAGTGCTGAC 
GAGTCTCAGG CCGTGCAAAT CCACATGGGC CTTGCTTTGG GGATGTTTCT CTCTCGTTTG 
TGTGAGGAGA AACTCAGTGA TATATCTGGC CAACAGATGA ACCTTCTTCT CATGAAGTCT 
TTGAATGCTC TGGAAAATTG CTGCTTCGAC ACCAGTCTTG AATACAACAC GGGCTGTATA 
CTGGGAGTTG GACTTGTTCT GTGTCTGATG AGCCACAGCA GCCACACGGA ATCCCGGGTC 
CACGTGGCAG CATCGCTTCG AAAGCTCTCT ACATACCTAG ATGACAGTGG GAGCCAGAGC 
AGAACGTTTC AGGAGGTCCT TGCCTATACC CTTAGCTGTG TATGTACGTC AGCGTTCAGT 
GCTGGAATTA TCGAGGCAGC AGAGGCTGAA GATATTATGA ATAAACTTCG GCTGTTAGTA 
GAGAACAACC AGCAGACTTC AGGTTTTGCC CTGGCATTAG GAAATATAGT TCATGGTTTG 
TCTGTGTGTG GACACGGAAA AGCTGAAGAC TTGGGCAACA AACTGCTTCC TGCCTGGATC 
AGAATTGTTC TATCAGAGGG CACTCCCACG ATGCTTTGTC TGGCAGCTCT TCATGGCATG 
GTGGCCTTGG TAGGCTCTGA AGGGGATGTA ATACAGCTGA AGTCAGAAGC CATCCAGACC 
TCTGAGTTTC AAGGCAGACT TAATGAAGTC ATTAGAACCT TAACTCAGGT CATCAGTGTC 
TCTGGGATGA TCGGCCTTCA GTCAAATGCA ATCTGGCTTC TAGGACATCT TCATCTATCT 
ACTCTCTCCT CAAATCAAAG TAGAAGCTCT GTTCCTACTG ACTATAGCTA TTTGCCTGAA 
AGCAGTTTTA TGAGAGCAGC CATTGGCTTC TTCGTTACGG GAGGAAAAAA AGGTCCTGAG 
TCTGTACCTC CTTCCCTTCT TAAAGTAGTG ATGAAGCCCA TAGCAACTGT TGGAGAAAAC 
TACCAATTCC CTCCTGTGAA CTGGGCTTCA CTCCTCTCAC CACTGATGAG ACTAAACTTT 
GGTGAAGAGA TACAGCAACT GTGCCTTGAA ATTATGGTGA CCCAGGCACA GTCATCACAG 
AATGCAGCTG CTCTGCTGGG CTTGTGGACA ATGCCACCAC TAATCCATGG TCTGAGTCTG 
AATATCAAGA AATACCTCCT GGTATCTGCA CCTCTGTGGA TAAAACATGT CTCTGAGGAA 
CAGCTCCTGG CTTTTGTTGA AAACTTAATG GTGGCAGTTT TTCAAGCTGC TCCCCGCATT 
TCAAGTACTG AGTTATGTCA GAATTCCTTA CAGGGTCTAA GCCAGGCCAT GAAACTGCCC 
AGCCCTGCCC ACCACCTCTG GAGTCTGCTC TGTGATGCTG TTGGGAAAAT TTTTGACCTT 
CTGCCAAATA AGATTCGGAG AAATGATATA GAGCTGTATA TCAGTGTAGC AAAATGCCTC 
TTGGAAATGA CAGATGACAA CATCAATCGA GTTGCCCAGA TTACTAAGAG CAACATAGAA 
AAGGCTGCCT TTGTCAAACT GTACTTAATC TCTCAAGGAC GATTCCCCTT GATGCACTTG 
TCTGATGTGC TCAGTGTCGC TGTGCAGCAC CAGGAGAGGG AGACACTGGC CTGGATGATA 
CTGCACAGCT TATACCAGGC GCGGATCGTG AGCCATGCCA ACACAGGCGT GTTGAAGCGA 
ATGGAGTGGC TCTTGGAAAT GATGGGTTAT ATTAGAAGTG TTGCTTACCA GTCAACATCT 
GTTCAGAATG TGGCTCTTGA TAAGGCCCTG GACTTCTTGT TGTTGATATT TGCAGCAGCA 
GTCGTTGCGT GGGCAGACCA TGCAGCCCCT CTTCTCCTTG GCCTCAGTGC CAGCTGGTTG 
CCATGGCATC TTGGCTCAGC TGGGTCAGCA CCCAGCTTCC TTGGCAGGAG TCCCATGCAC 
AGGGTCATTC TGGAGGAGGT TCTCACTCTC CTTCCTAGTA GCATGCCTCT TCTGCTGCAG 
AAGGAGCCAT GGAAGGAGCA GACTCAGAAG TTCATTGACT GGCTATTCAA CATCATGGAA 
GCCCCCAAAG AAGCCCTTTC AGCAAAATCC AGGAATCTGT TAAAAGCCAC CCTCCTGTCC 
TTGAGAGTTC TCCCAGAGTT TAAGAAGAAA GCTGTATGGA CCAGAGCATA TGGTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101340282 ( XM_012554721.2 ) cDNA ORF clone, Trichechus manatus latirostris(Florida manatee) -> XP_012410175.2 Trichechus manatus latirostris focadhesin (LOC101340282), mRNA.

Price & Availability↑

CloneID	OTr217144
Clone ID Related Accession (Same CDS sequence)	XM_012554721.2
Accession Version	XM_012554721.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-24
Organism	Trichechus manatus latirostris(Florida manatee)
Product	focadhesin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004443961.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 25, 2018 this sequence version replaced XM_012554721.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Trichechus manatus latirostris Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA GTCACAGGCT 
GTGGGTCATC TTATTGCTGC AGTCCTAAAG GAAAATGGTT ATTCAGAAAA AATCCACCAG 
TCTACAAACC AGACTCCTGC TTTGAACTTG CTGTGGGAAA AGTGTTGCAG TGACAACGTG 
GTGGTTCGAA CAGCCTGCTG TGAGGGTTTG GTGGCACTTG TTGCTCAGGA TCATGCGGAA 
TTCAGCTATG TTCTCAATGG GATACTCAAC CTGATTCCCT CGACCAGGAA TACCCATGGC 
TTGATAAAAG CCATTGTGAA ATTATTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAGAAGAATA TTCAGGATAT ATATACTATT AGGTTTTGTG TGAGACTCAG AGTCAGCATG 
TTTATCCAGC TGCTCAGCGA TATGAACATA GGTCATTTGT GGACCACATT TGAGACATAC 
TGCTTTAGCC CCTGGGAGCT TGACATTACC ACAGCTAATC ACTATGTGAA TTACAGGTCA 
GAGATTCCAT GTGTCCAAAT AATGGCACCA TTTCTATGGT ATTTATATTG TGAACCATCC 
CAGTTACAAG AATATGCTAA ACTTCGACTA GCCCTGCTAA AAGTCTTACT TCAACCCTCC 
GTTCTTTGTG AGAAAGAACA ACCATCAGTA TTGGAACAAC ACATACTTCA ACTGTGTTGT 
GACATGGTTC CGTGTTTGCA GGACTTTCCT GTTGAACTGG TCTTAAGTGG AATAGCTTTA 
CTTCTTCTGC AGACTCCAGC AAGTCAACAG AGGCCGATCT TAAGTCTAGC TTTGAAGCTC 
CTCTCTTTTG CTGAGGGTCA GAAAATCCCA AAGTCATCCC TGCTGCTGGT AATGCCAGTT 
CTGCAGATTT TATCGTCTAC TGCCTTGGAA GACTGTATAT CTGTGGATGA AGAAGGGCCC 
TCTAGGCAGC AGTTGGCTGT AAACCTTTTG GAAATGGTAC AGCAGGAGTG CTACAGAGAC 
GACAGCCAAA AGGTTCCAAG TCTGATTCCA CTTTTGATGT TCAAATTAGG AAGACCACTG 
GAACCTGTAT CGTACAATGA TATCTTGTAT ACTTTACCTA CTCTCGGTGT TCACAAGGTG 
TGCATAGGAC AAATTCTACG AGTAATCCAA CTGCTTGGAA CCACCCCACA GCTAAGAGCT 
GTCACTTTGC GCTTGTTGAC ATCTTTGTGG GAAAAACAGG ATCGTGTCTA TCCTGAGCTG 
CAGCGGTTCA TGGCCATGTC TGATGTGCCT TCTCTGTCTG TGGGCAGGGA GGTCCAGTGG 
GAGAAACTAA TCGCGAAAGC AGCATCGATC AGAGATATAT GTAAGCAAAG ACCATATCAG 
CATGGTGCTG ATATGTTGGC AGCTATTTCT CAGGTGTTAA ACGAATGCAC TAAATCTGAT 
CAAGCTACTC CAGCAGCCTT GGTGTTACAA GGTCTTCATG CGCTCTGTCA AGCTGAGGTT 
GTTTGTATTC GCTCTACTTG GAATGCTCTC TCCCCAAAAC TGAGTTGTGA CACGAGACCT 
CTCATTTTAA AGACCCTCAG TGAACTCTTT TCTCTGGTTC CTTCCTTAAC AGTCAATACA 
GCTGAATATG AGAATTTTAA AGTTCAAGTC CTCAGCTTCC TCTGGACTCA AACTCAAAAC 
AAGGACCCAG TTGTAGCAAA TGCTGCATAT AAATCTCTGT CACAGTTTAG CGCAGGGGAG 
CACACCATTC TTCATCTGCC TGAAAAGATA AGACCAGAAA TTCCTGTTTC TGATGAGCTA 
GATGAAGAAG AAGATGATGA GGGTGATGAA AAGGAAGTGG ACCTTTCAAT TCCTGGTTTT 
TGCTATCTCA AACTGTTGTC ACTTACTGCT CCTTCAGTTT TACCAGCTTT GGAGGAATTT 
TTTACATCAC TTGTGAAACA AGAAATGGTG AATATGCCCC GTGGGGTGTA TCACTCTGCA 
TTAAAAGGAG GTGTCCGTTC AGATCAAGGA AAGACTATTG CAGGAATCCC CAATTTTATG 
TTGAAAATGT ATGAAACAAA CAAGCAACCA GGATTGAAAC CTGGCCTTGC CGGTGGCATG 
TTATTTTGTT ACGATATTTC AATGTATCAG AGTAAAGATG GCAAACCATT GAATCGATTG 
ATGGCCAGTA GAGGGCGAAG TTTCAAGCAG ACATCACTTG CTCTTGTGCA TGAGGTTCAC 
ATTCAGCTCT TGGAGTGGCA CCGTGCAGTA TTTCTCCCAC AGGCCTGGCT TGCATATATG 
AATCGAGCAT ATCATGCCAT TTTACAGGGA AGAATAGCAG AGTTGGAGTT ACAGTTAAAA 
CACGGAAAAG AAGGACCTGA GGAGGTGCAG TACAAGAAAA GCACAGCCTG GCTCTGGGTC 
AGAGACATGT TGACTGATGA GATCACCAAG GCAGCTGCAA AGGACAGCCC AGTGGTCAAA 
GGGAACGCAC TGTTAGCCTT AAGTAGCCTT GCTGTTGTCG TATCCAAACA TGAAGCCAGC 
CTCTCCTCAG ACTCGGATGA GGCCCTGGAG ATTCAACCTA ACTTCCTTTC AATGAAAGAG 
TGGGTTTCCA TGGTGCTTGA TACCCTCCTG GTAATTGTGG ATAGTCATTA CCAACCCCGA 
GGACAACTCT TCTCCTGGTT TTATTATAAA TCCTATTCCG GTGAAAACAC AGCTAGTGCC 
ATTGCCCGTT CTGCTGCCGC CACGGCTTTG TCTCTCCTCG TGCCAGTTTT CATTATCTCT 
TGCAAAGAGA AGGTTGAGGA AATCCTGAAC ATGCTGACTG CCAGGTTACC TGGGAAACCA 
AGTGCTGACG AGTCTCAGGC CGTGCAAATC CACATGGGCC TTGCTTTGGG GATGTTTCTC 
TCTCGTTTGT GTGAGGAGAA ACTCAGTGAT ATATCTGGCC AACAGATGAA CCTTCTTCTC 
ATGAAGTCTT TGAATGCTCT GGAAAATTGC TGCTTCGACA CCAGTCTTGA ATACAACACG 
GGCTGTATAC TGGGAGTTGG ACTTGTTCTG TGTCTGATGA GCCACAGCAG CCACACGGAA 
TCCCGGGTCC ACGTGGCAGC ATCGCTTCGA AAGCTCTCTA CATACCTAGA TGACAGTGGG 
AGCCAGAGCA GAACGTTTCA GGAGGTCCTT GCCTATACCC TTAGCTGTGT ATGTACGTCA 
GCGTTCAGTG CTGGAATTAT CGAGGCAGCA GAGGCTGAAG ATATTATGAA TAAACTTCGG 
CTGTTAGTAG AGAACAACCA GCAGACTTCA GGTTTTGCCC TGGCATTAGG AAATATAGTT 
CATGGTTTGT CTGTGTGTGG ACACGGAAAA GCTGAAGACT TGGGCAACAA ACTGCTTCCT 
GCCTGGATCA GAATTGTTCT ATCAGAGGGC ACTCCCACGA TGCTTTGTCT GGCAGCTCTT 
CATGGCATGG TGGCCTTGGT AGGCTCTGAA GGGGATGTAA TACAGCTGAA GTCAGAAGCC 
ATCCAGACCT CTGAGTTTCA AGGCAGACTT AATGAAGTCA TTAGAACCTT AACTCAGGTC 
ATCAGTGTCT CTGGGATGAT CGGCCTTCAG TCAAATGCAA TCTGGCTTCT AGGACATCTT 
CATCTATCTA CTCTCTCCTC AAATCAAAGT AGAAGCTCTG TTCCTACTGA CTATAGCTAT 
TTGCCTGAAA GCAGTTTTAT GAGAGCAGCC ATTGGCTTCT TCGTTACGGG AGGAAAAAAA 
GGTCCTGAGT CTGTACCTCC TTCCCTTCTT AAAGTAGTGA TGAAGCCCAT AGCAACTGTT 
GGAGAAAACT ACCAATTCCC TCCTGTGAAC TGGGCTTCAC TCCTCTCACC ACTGATGAGA 
CTAAACTTTG GTGAAGAGAT ACAGCAACTG TGCCTTGAAA TTATGGTGAC CCAGGCACAG 
TCATCACAGA ATGCAGCTGC TCTGCTGGGC TTGTGGACAA TGCCACCACT AATCCATGGT 
CTGAGTCTGA ATATCAAGAA ATACCTCCTG GTATCTGCAC CTCTGTGGAT AAAACATGTC 
TCTGAGGAAC AGCTCCTGGC TTTTGTTGAA AACTTAATGG TGGCAGTTTT TCAAGCTGCT 
CCCCGCATTT CAAGTACTGA GTTATGTCAG AATTCCTTAC AGGGTCTAAG CCAGGCCATG 
AAACTGCCCA GCCCTGCCCA CCACCTCTGG AGTCTGCTCT GTGATGCTGT TGGGAAAATT 
TTTGACCTTC TGCCAAATAA GATTCGGAGA AATGATATAG AGCTGTATAT CAGTGTAGCA 
AAATGCCTCT TGGAAATGAC AGATGACAAC ATCAATCGAG TTGCCCAGAT TACTAAGAGC 
AACATAGAAA AGGCTGCCTT TGTCAAACTG TACTTAATCT CTCAAGGACG ATTCCCCTTG 
ATGCACTTGT CTGATGTGCT CAGTGTCGCT GTGCAGCACC AGGAGAGGGA GACACTGGCC 
TGGATGATAC TGCACAGCTT ATACCAGGCG CGGATCGTGA GCCATGCCAA CACAGGCGTG 
TTGAAGCGAA TGGAGTGGCT CTTGGAAATG ATGGGTTATA TTAGAAGTGT TGCTTACCAG 
TCAACATCTG TTCAGAATGT GGCTCTTGAT AAGGCCCTGG ACTTCTTGTT GTTGATATTT 
GCAGCAGCAG TCGTTGCGTG GGCAGACCAT GCAGCCCCTC TTCTCCTTGG CCTCAGTGCC 
AGCTGGTTGC CATGGCATCT TGGCTCAGCT GGGTCAGCAC CCAGCTTCCT TGGCAGGAGT 
CCCATGCACA GGGTCATTCT GGAGGAGGTT CTCACTCTCC TTCCTAGTAG CATGCCTCTT 
CTGCTGCAGA AGGAGCCATG GAAGGAGCAG ACTCAGAAGT TCATTGACTG GCTATTCAAC 
ATCATGGAAG CCCCCAAAGA AGCCCTTTCA GCAAAATCCA GGAATCTGTT AAAAGCCACC 
CTCCTGTCCT TGAGAGTTCT CCCAGAGTTT AAGAAGAAAG CTGTATGGAC CAGAGCATAT 
GGTTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012410175.2
CDS	45..5033
Translation	MSDDIRKRFEFPNSLIQSQAVGHLIAAVLKENGYSEKIHQSTNQTPALNLLWEKCCSDNVVVRTACCEGLVALVAQDHAEFSYVLNGILNLIPSTRNTHGLIKAIVKLLQMQALKEGQGGEKNIQDIYTIRFCVRLRVSMFIQLLSDMNIGHLWTTFETYCFSPWELDITTANHYVNYRSEIPCVQIMAPFLWYLYCEPSQLQEYAKLRLALLKVLLQPSVLCEKEQPSVLEQHILQLCCDMVPCLQDFPVELVLSGIALLLLQTPASQQRPILSLALKLLSFAEGQKIPKSSLLLVMPVLQILSSTALEDCISVDEEGPSRQQLAVNLLEMVQQECYRDDSQKVPSLIPLLMFKLGRPLEPVSYNDILYTLPTLGVHKVCIGQILRVIQLLGTTPQLRAVTLRLLTSLWEKQDRVYPELQRFMAMSDVPSLSVGREVQWEKLIAKAASIRDICKQRPYQHGADMLAAISQVLNECTKSDQATPAALVLQGLHALCQAEVVCIRSTWNALSPKLSCDTRPLILKTLSELFSLVPSLTVNTAEYENFKVQVLSFLWTQTQNKDPVVANAAYKSLSQFSAGEHTILHLPEKIRPEIPVSDELDEEEDDEGDEKEVDLSIPGFCYLKLLSLTAPSVLPALEEFFTSLVKQEMVNMPRGVYHSALKGGVRSDQGKTIAGIPNFMLKMYETNKQPGLKPGLAGGMLFCYDISMYQSKDGKPLNRLMASRGRSFKQTSLALVHEVHIQLLEWHRAVFLPQAWLAYMNRAYHAILQGRIAELELQLKHGKEGPEEVQYKKSTAWLWVRDMLTDEITKAAAKDSPVVKGNALLALSSLAVVVSKHEASLSSDSDEALEIQPNFLSMKEWVSMVLDTLLVIVDSHYQPRGQLFSWFYYKSYSGENTASAIARSAAATALSLLVPVFIISCKEKVEEILNMLTARLPGKPSADESQAVQIHMGLALGMFLSRLCEEKLSDISGQQMNLLLMKSLNALENCCFDTSLEYNTGCILGVGLVLCLMSHSSHTESRVHVAASLRKLSTYLDDSGSQSRTFQEVLAYTLSCVCTSAFSAGIIEAAEAEDIMNKLRLLVENNQQTSGFALALGNIVHGLSVCGHGKAEDLGNKLLPAWIRIVLSEGTPTMLCLAALHGMVALVGSEGDVIQLKSEAIQTSEFQGRLNEVIRTLTQVISVSGMIGLQSNAIWLLGHLHLSTLSSNQSRSSVPTDYSYLPESSFMRAAIGFFVTGGKKGPESVPPSLLKVVMKPIATVGENYQFPPVNWASLLSPLMRLNFGEEIQQLCLEIMVTQAQSSQNAAALLGLWTMPPLIHGLSLNIKKYLLVSAPLWIKHVSEEQLLAFVENLMVAVFQAAPRISSTELCQNSLQGLSQAMKLPSPAHHLWSLLCDAVGKIFDLLPNKIRRNDIELYISVAKCLLEMTDDNINRVAQITKSNIEKAAFVKLYLISQGRFPLMHLSDVLSVAVQHQERETLAWMILHSLYQARIVSHANTGVLKRMEWLLEMMGYIRSVAYQSTSVQNVALDKALDFLLLIFAAAVVAWADHAAPLLLGLSASWLPWHLGSAGSAPSFLGRSPMHRVILEEVLTLLPSSMPLLLQKEPWKEQTQKFIDWLFNIMEAPKEALSAKSRNLLKATLLSLRVLPEFKKKAVWTRAYGW

Target ORF information:

RefSeq Version	XM_012554721.2
Organism	Trichechus manatus latirostris(Florida manatee)
Definition	Trichechus manatus latirostris focadhesin (LOC101340282), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012554721.2

ORF Insert Sequence:

ATGTCAGATG ATATCAGGAA AAGGTTTGAA TTTCCAAATT CTCTTATCCA GTCACAGGCT 
GTGGGTCATC TTATTGCTGC AGTCCTAAAG GAAAATGGTT ATTCAGAAAA AATCCACCAG 
TCTACAAACC AGACTCCTGC TTTGAACTTG CTGTGGGAAA AGTGTTGCAG TGACAACGTG 
GTGGTTCGAA CAGCCTGCTG TGAGGGTTTG GTGGCACTTG TTGCTCAGGA TCATGCGGAA 
TTCAGCTATG TTCTCAATGG GATACTCAAC CTGATTCCCT CGACCAGGAA TACCCATGGC 
TTGATAAAAG CCATTGTGAA ATTATTACAA ATGCAAGCTC TTAAGGAAGG ACAAGGTGGG 
GAGAAGAATA TTCAGGATAT ATATACTATT AGGTTTTGTG TGAGACTCAG AGTCAGCATG 
TTTATCCAGC TGCTCAGCGA TATGAACATA GGTCATTTGT GGACCACATT TGAGACATAC 
TGCTTTAGCC CCTGGGAGCT TGACATTACC ACAGCTAATC ACTATGTGAA TTACAGGTCA 
GAGATTCCAT GTGTCCAAAT AATGGCACCA TTTCTATGGT ATTTATATTG TGAACCATCC 
CAGTTACAAG AATATGCTAA ACTTCGACTA GCCCTGCTAA AAGTCTTACT TCAACCCTCC 
GTTCTTTGTG AGAAAGAACA ACCATCAGTA TTGGAACAAC ACATACTTCA ACTGTGTTGT 
GACATGGTTC CGTGTTTGCA GGACTTTCCT GTTGAACTGG TCTTAAGTGG AATAGCTTTA 
CTTCTTCTGC AGACTCCAGC AAGTCAACAG AGGCCGATCT TAAGTCTAGC TTTGAAGCTC 
CTCTCTTTTG CTGAGGGTCA GAAAATCCCA AAGTCATCCC TGCTGCTGGT AATGCCAGTT 
CTGCAGATTT TATCGTCTAC TGCCTTGGAA GACTGTATAT CTGTGGATGA AGAAGGGCCC 
TCTAGGCAGC AGTTGGCTGT AAACCTTTTG GAAATGGTAC AGCAGGAGTG CTACAGAGAC 
GACAGCCAAA AGGTTCCAAG TCTGATTCCA CTTTTGATGT TCAAATTAGG AAGACCACTG 
GAACCTGTAT CGTACAATGA TATCTTGTAT ACTTTACCTA CTCTCGGTGT TCACAAGGTG 
TGCATAGGAC AAATTCTACG AGTAATCCAA CTGCTTGGAA CCACCCCACA GCTAAGAGCT 
GTCACTTTGC GCTTGTTGAC ATCTTTGTGG GAAAAACAGG ATCGTGTCTA TCCTGAGCTG 
CAGCGGTTCA TGGCCATGTC TGATGTGCCT TCTCTGTCTG TGGGCAGGGA GGTCCAGTGG 
GAGAAACTAA TCGCGAAAGC AGCATCGATC AGAGATATAT GTAAGCAAAG ACCATATCAG 
CATGGTGCTG ATATGTTGGC AGCTATTTCT CAGGTGTTAA ACGAATGCAC TAAATCTGAT 
CAAGCTACTC CAGCAGCCTT GGTGTTACAA GGTCTTCATG CGCTCTGTCA AGCTGAGGTT 
GTTTGTATTC GCTCTACTTG GAATGCTCTC TCCCCAAAAC TGAGTTGTGA CACGAGACCT 
CTCATTTTAA AGACCCTCAG TGAACTCTTT TCTCTGGTTC CTTCCTTAAC AGTCAATACA 
GCTGAATATG AGAATTTTAA AGTTCAAGTC CTCAGCTTCC TCTGGACTCA AACTCAAAAC 
AAGGACCCAG TTGTAGCAAA TGCTGCATAT AAATCTCTGT CACAGTTTAG CGCAGGGGAG 
CACACCATTC TTCATCTGCC TGAAAAGATA AGACCAGAAA TTCCTGTTTC TGATGAGCTA 
GATGAAGAAG AAGATGATGA GGGTGATGAA AAGGAAGTGG ACCTTTCAAT TCCTGGTTTT 
TGCTATCTCA AACTGTTGTC ACTTACTGCT CCTTCAGTTT TACCAGCTTT GGAGGAATTT 
TTTACATCAC TTGTGAAACA AGAAATGGTG AATATGCCCC GTGGGGTGTA TCACTCTGCA 
TTAAAAGGAG GTGTCCGTTC AGATCAAGGA AAGACTATTG CAGGAATCCC CAATTTTATG 
TTGAAAATGT ATGAAACAAA CAAGCAACCA GGATTGAAAC CTGGCCTTGC CGGTGGCATG 
TTATTTTGTT ACGATATTTC AATGTATCAG AGTAAAGATG GCAAACCATT GAATCGATTG 
ATGGCCAGTA GAGGGCGAAG TTTCAAGCAG ACATCACTTG CTCTTGTGCA TGAGGTTCAC 
ATTCAGCTCT TGGAGTGGCA CCGTGCAGTA TTTCTCCCAC AGGCCTGGCT TGCATATATG 
AATCGAGCAT ATCATGCCAT TTTACAGGGA AGAATAGCAG AGTTGGAGTT ACAGTTAAAA 
CACGGAAAAG AAGGACCTGA GGAGGTGCAG TACAAGAAAA GCACAGCCTG GCTCTGGGTC 
AGAGACATGT TGACTGATGA GATCACCAAG GCAGCTGCAA AGGACAGCCC AGTGGTCAAA 
GGGAACGCAC TGTTAGCCTT AAGTAGCCTT GCTGTTGTCG TATCCAAACA TGAAGCCAGC 
CTCTCCTCAG ACTCGGATGA GGCCCTGGAG ATTCAACCTA ACTTCCTTTC AATGAAAGAG 
TGGGTTTCCA TGGTGCTTGA TACCCTCCTG GTAATTGTGG ATAGTCATTA CCAACCCCGA 
GGACAACTCT TCTCCTGGTT TTATTATAAA TCCTATTCCG GTGAAAACAC AGCTAGTGCC 
ATTGCCCGTT CTGCTGCCGC CACGGCTTTG TCTCTCCTCG TGCCAGTTTT CATTATCTCT 
TGCAAAGAGA AGGTTGAGGA AATCCTGAAC ATGCTGACTG CCAGGTTACC TGGGAAACCA 
AGTGCTGACG AGTCTCAGGC CGTGCAAATC CACATGGGCC TTGCTTTGGG GATGTTTCTC 
TCTCGTTTGT GTGAGGAGAA ACTCAGTGAT ATATCTGGCC AACAGATGAA CCTTCTTCTC 
ATGAAGTCTT TGAATGCTCT GGAAAATTGC TGCTTCGACA CCAGTCTTGA ATACAACACG 
GGCTGTATAC TGGGAGTTGG ACTTGTTCTG TGTCTGATGA GCCACAGCAG CCACACGGAA 
TCCCGGGTCC ACGTGGCAGC ATCGCTTCGA AAGCTCTCTA CATACCTAGA TGACAGTGGG 
AGCCAGAGCA GAACGTTTCA GGAGGTCCTT GCCTATACCC TTAGCTGTGT ATGTACGTCA 
GCGTTCAGTG CTGGAATTAT CGAGGCAGCA GAGGCTGAAG ATATTATGAA TAAACTTCGG 
CTGTTAGTAG AGAACAACCA GCAGACTTCA GGTTTTGCCC TGGCATTAGG AAATATAGTT 
CATGGTTTGT CTGTGTGTGG ACACGGAAAA GCTGAAGACT TGGGCAACAA ACTGCTTCCT 
GCCTGGATCA GAATTGTTCT ATCAGAGGGC ACTCCCACGA TGCTTTGTCT GGCAGCTCTT 
CATGGCATGG TGGCCTTGGT AGGCTCTGAA GGGGATGTAA TACAGCTGAA GTCAGAAGCC 
ATCCAGACCT CTGAGTTTCA AGGCAGACTT AATGAAGTCA TTAGAACCTT AACTCAGGTC 
ATCAGTGTCT CTGGGATGAT CGGCCTTCAG TCAAATGCAA TCTGGCTTCT AGGACATCTT 
CATCTATCTA CTCTCTCCTC AAATCAAAGT AGAAGCTCTG TTCCTACTGA CTATAGCTAT 
TTGCCTGAAA GCAGTTTTAT GAGAGCAGCC ATTGGCTTCT TCGTTACGGG AGGAAAAAAA 
GGTCCTGAGT CTGTACCTCC TTCCCTTCTT AAAGTAGTGA TGAAGCCCAT AGCAACTGTT 
GGAGAAAACT ACCAATTCCC TCCTGTGAAC TGGGCTTCAC TCCTCTCACC ACTGATGAGA 
CTAAACTTTG GTGAAGAGAT ACAGCAACTG TGCCTTGAAA TTATGGTGAC CCAGGCACAG 
TCATCACAGA ATGCAGCTGC TCTGCTGGGC TTGTGGACAA TGCCACCACT AATCCATGGT 
CTGAGTCTGA ATATCAAGAA ATACCTCCTG GTATCTGCAC CTCTGTGGAT AAAACATGTC 
TCTGAGGAAC AGCTCCTGGC TTTTGTTGAA AACTTAATGG TGGCAGTTTT TCAAGCTGCT 
CCCCGCATTT CAAGTACTGA GTTATGTCAG AATTCCTTAC AGGGTCTAAG CCAGGCCATG 
AAACTGCCCA GCCCTGCCCA CCACCTCTGG AGTCTGCTCT GTGATGCTGT TGGGAAAATT 
TTTGACCTTC TGCCAAATAA GATTCGGAGA AATGATATAG AGCTGTATAT CAGTGTAGCA 
AAATGCCTCT TGGAAATGAC AGATGACAAC ATCAATCGAG TTGCCCAGAT TACTAAGAGC 
AACATAGAAA AGGCTGCCTT TGTCAAACTG TACTTAATCT CTCAAGGACG ATTCCCCTTG 
ATGCACTTGT CTGATGTGCT CAGTGTCGCT GTGCAGCACC AGGAGAGGGA GACACTGGCC 
TGGATGATAC TGCACAGCTT ATACCAGGCG CGGATCGTGA GCCATGCCAA CACAGGCGTG 
TTGAAGCGAA TGGAGTGGCT CTTGGAAATG ATGGGTTATA TTAGAAGTGT TGCTTACCAG 
TCAACATCTG TTCAGAATGT GGCTCTTGAT AAGGCCCTGG ACTTCTTGTT GTTGATATTT 
GCAGCAGCAG TCGTTGCGTG GGCAGACCAT GCAGCCCCTC TTCTCCTTGG CCTCAGTGCC 
AGCTGGTTGC CATGGCATCT TGGCTCAGCT GGGTCAGCAC CCAGCTTCCT TGGCAGGAGT 
CCCATGCACA GGGTCATTCT GGAGGAGGTT CTCACTCTCC TTCCTAGTAG CATGCCTCTT 
CTGCTGCAGA AGGAGCCATG GAAGGAGCAG ACTCAGAAGT TCATTGACTG GCTATTCAAC 
ATCATGGAAG CCCCCAAAGA AGCCCTTTCA GCAAAATCCA GGAATCTGTT AAAAGCCACC 
CTCCTGTCCT TGAGAGTTCT CCCAGAGTTT AAGAAGAAAG CTGTATGGAC CAGAGCATAT 
GGTTGGTGA