LOC101066527 gene cDNA ORF clone, Takifugu rubripes(torafugu)

mRNA	Protein	Name
XM_011617371.1	XP_011615673.1	collagen alpha-2(XI) chain isoform X6
XM_011617365.2	XP_011615667.2	collagen alpha-1(XI) chain isoform X1
XM_011617365.1	XP_011615667.1	collagen alpha-2(XI) chain isoform X1
XM_011617367.1	XP_011615669.1	collagen alpha-2(XI) chain isoform X3
XM_011617370.1	XP_011615672.1	collagen alpha-2(XI) chain isoform X5
XM_011617366.2	XP_011615668.2	collagen alpha-1(XI) chain isoform X2
XM_011617369.1	XP_011615671.1	collagen alpha-2(XI) chain isoform X4
XM_011617366.1	XP_011615668.1	collagen alpha-2(XI) chain isoform X2
XM_011617367.2	XP_011615669.2	collagen alpha-1(V) chain isoform X3
XM_011617369.2	XP_011615671.2	collagen alpha-2(XI) chain isoform X4
XM_011617370.2	XP_011615672.2	collagen alpha-2(XI) chain isoform X5
XM_011617371.2	XP_011615673.2	collagen alpha-2(XI) chain isoform X6
XM_029844529.1	XP_029700389.1	collagen alpha-1(XI) chain isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo45740	XM_011617369.2 Latest version!	Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo45741	XM_011617370.2 Latest version!	Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo45742	XM_011617371.2 Latest version!	Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OTo45743	XM_029844529.1 Latest version!	Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OTo45737	XM_011617365.2 Latest version!	Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo45738	XM_011617366.2 Latest version!	Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo45739	XM_011617367.2 Latest version!	Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OTo22616	XM_011617365.1 Latest version!	Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OTo22617	XM_011617366.1 Latest version!	Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OTo22618	XM_011617367.1 Latest version!	Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
OTo22619	XM_011617369.1 Latest version!	Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OTo22620	XM_011617370.1 Latest version!	Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OTo22621	XM_011617371.1 Latest version!	Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

LOC101066527 ( XM_011617369.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011615671.2 Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTo45740
Clone ID Related Accession (Same CDS sequence)	XM_011617369.2
Accession Version	XM_011617369.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	collagen alpha-2(XI) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042296.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011617369.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGAC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATTAC 
TTTGATGAGG AGCAGGATGA CCATGAATAC CCTTATTATT ATGAGGAAAC AACAGGCCGT 
CCCTCCTCCT CTGCACCCCC CTCTCCCCAA CCTGGGGCCC CCAGTCAACA GGATGTAGAC 
ACTGAATATT CCGAGGCTGG CCACACCCCT ACAGAGAGAG ATTATTACTA TGAGGAGACG 
GTACCACTGC CAGAGGGTGA GGTGGTTGGG CAAGAGGAAC TCGCCGTACA GCCCCAGGTG 
GAGCCTGTGT TGGAGGGAGA AGAGGTGCAT TCGACCAAGG CTGTCGGTGA GGGAGAGGAG 
GCCTTCACAG AGGAGTATGT GACTGGAGAT GTGACCCTTA AGGAATACGA CTATTCCTAC 
AGGGACTACA ATGAGCCATT AGTGGAGACT GGAGAAGTCG ATGCCAACAT GGGCCCCGCC 
CTCTCTGCTG TGACAGACGA GGGAGGCGCT GGGGTGAGAG GCCAGAAAGG AGAGAAAGGA 
GAACCTGCTG TTTTGGAGCC TGGGATGCTG ATTGAAGGAC CACCAGGACC TGAGGGACCT 
GCTGGCCTAC CTGGTCCTTC TGGCCCTTCT GGACCTCCTG GCTCTGTGGG TGACCCAGGA 
GAGAGGGGCC CTCCTGGTAA GGCTGGTCTT CCTGGAGCTG ATGGAGTCCC TGGACCTCCT 
GGGACCTCTG TTATGCTGCC TTTCCGTTTT GGTCAGAGTG GAGGAGATAA AGGTCCTGCT 
GTTTCTGCTC AGGAAGCTCA GGCAGCAGCC ATCTTGTCTC AGGCCAGGAT GGCTCTTAAA 
GGACCTCCTG GTCCAATGGG ATTCACTGGG CGCCCCGGAC CCTTGGGAAA TCCTGGAAGT 
CCTGGTCTGA AAGGAGAGAG TGGAGACCCT GGACCCCAGG GCCCGAGGGG ATCTCTAGGC 
TTGATGGGAC CTCCAGGCAA ATCAGGAAGA AGAGGCCGCG CTGGAGCCGA CGGTGCCAGA 
GGAATGCCTG GAGAGTCTGG TTCTAAGGGT GATCGTGGTT TTGATGGTCT TCCTGGTTTA 
CCTGGAGACA AAGGACACAG GGGTGACTCG GGACCAATGG GACCTCCAGG ATCTCAGGGA 
GAGGATGGAG AAAGGGGAGA CGACGGAGAC GTTGGACCCC GGGGTCTTCC AGGTGAACCA 
GGACCTCGTG GTTTGCTTGG ACCTAAAGGT CCTCCTGGAA TCCCAGGCCC TCCTGGTGTA 
CGAGGAAGTG ACGGACCCCA CGGTCCAAAA GGAAACTTGG GTCCTCAAGG TGAGCCAGGA 
CCCCCTGGTC AGCAGGGAAC CCCAGGAACT CAGGGAATGC CAGGACCTCA AGGACATACT 
GGACCTCCAG GAGAAAAGGG TCCAACAGGA AAACCGGGTT TACCAGGAAT GCCTGGAGCT 
GATGGCCCTC CTGGTCACCC AGGAAAGGAA GGCCCATCAG GCACCAAAGG AAACCAGGGT 
CCCAGTGGTC CCCAGGGGCC TATTGGCTAT CCTGGTCCTC GTGGCCTCAA GGGCGGTCAA 
GGAATCCGGG GATTGAAGGG TCACAAAGGA GAGAAGGGAG AAGATGGTTT CCCTGGAATT 
AAGGGAGACT TTGGGATCAA GGGTGAGAGG GGTGAGATTG GAGTACCAGG TCCTAGAGGA 
GAGGACGGTC CAGAGGGGCC AAAAGGTCGT TTTGGTCCCC CTGGTGAAAC TGGACCACTC 
GGAGCAGTAG GAGAGAAGGG TAAACTTGGT GTACCGGGAC TTCCTGGATA TCCTGGAAGA 
CAAGGACCAA AGGGATCTCT TGGATTCCCA GGATTCCCTG GATCCAATGG CGAGAAGGGA 
ACAAGGGGTC TCACTGGCAA ATCAGGACCA AGAGGACAAA GAGGACCAAC GGGCCCCAGA 
GGGCAGAGGG GACCGAGGGG CGCAACTGGC AAACTGGGGC CAAAGGGGAC TTCGGGAAGT 
GATGGCCCCC CTGGTCCTCC TGGAGAAAGG GGCTTGCCTG GGCCTCAGGG AGCCAATGGG 
TTCCCCGGAC CTAAAGGGCC ACCGGGGCCA CCAGGGAAGG ACGGGCTGCC TGGACACCCT 
GGGCAGAGAG GAGAAGTGGG TTTCCAAGGT AAAATGGGTC CACCTGGGCC TCCTGGAGTT 
GTTGGACCTC AGGGTCCAGC TGGAGAGACT GGCCCTAATG GTGAACGTGG CCACCCTGGA 
CCACCAGGTC CACCAGGAGA ACAGGGACTT CCTGGTCCCT CAGGAAAGGA GGGAACCAAA 
GGAGACCCTG GACCCCCAGG AGGCCCTGGC AAAGATGGGC CTCCTGGACT GAGGGGCTTC 
CCTGGTGAGA GAGGACTGCC AGGAACACCT GGAGGTGGAG GATTCAAAGG AAGCGAAGGA 
CCTGCTGGAC CTCCTGGACC GGCTGGCTCT CCTGGTGAGA GGGGGCAATC TGGTACCGCT 
GGGCCTGTTG GACCTCCTGG AAGACCAGGT CCTCAGGGAC CTCCTGGACC TGCAGGAGAG 
AAAGGTGTTT CTGGTGAGAA AGGCCCAATT GGCCCAGCTG GTCGTGACGG ACTACAGGGT 
CCTGTGGGTC TGCCTGGCCC AGCTGGACCG CCCGGAGTAC CTGGAGAAGA TGGAGATAAG 
GGGGAGGTTG GAGAACACGG TCAGAAAGGT GCCAAGGGAG GGAAAGGAGA GCATGGTCCT 
CCAGGTCCAC CTGGACCAAT GGGACCAGTC GGCCAGCCTG GTGCTCCAGG TGCTGATGGA 
GAGCTTGGGC CGAGGGGTCA GCAGGGACCT TTTGGAGCTA AAGGGGATGA AGGAACCAGA 
GGATTCCCAG GAGCCCCAGG ACCCATTGGA CTCCAGGGTT TGCCTGGGCC ACCAGGTGAG 
AAGGGAGAAA CCGGAGATGT TGGACCTCTG GGACCTCCAG GCCCTCCAGG ACCCCGTGGC 
CCTGCTGGAC CCAACGGTGC TGATGGCCCT CAAGGGCCTC CTGGTGGTTT GGGTAATCCT 
GGACCTATCG GTGAGAAGGG AGAGCCTGGA GAGTCTGGAC CCCCTGGAAT TGGAGGGGAG 
CCTGGAAAGA AGGGCCCCAG AGGGGAACGT GGGGAGAAGG GGGAGGCTGG ACAACCAGGA 
ACCGCTGGAT CCACTGGAGG ACGTGGACTA CCTGGAGATG ATGGACCCAA AGGAAACCCA 
GGTCCTGTTG GTTTCCCCGG TGATCCTGGT CCACCGGGTG AGGTTGGACC CAGAGGTCAA 
GACGGTGCCA AGGGAGAAAG AGGAGAAGAT GGTGAAGCAG GAGAGTCTGG ATCCCCTGGA 
CCTCCCGGTG AGAACGGACC CCCCGGCCCC CTAGGAAAGA GGGGTCCTGC TGGAACAAGA 
GGACCAGAGG GACGCCAGGG AGAGAAGGGA ACAAAGGGGG ATCCAGGTGC TGTTGGCCCC 
CCAGGCAAAA CAGGCCCTGT GGGTCCACAG GGAGTGCCAG GAAAACCTGG AACAGAAGGC 
CTTAGAGGGC TTCCAGGATC AGTGGGTGAG CAAGGATCTC CAGGACCTGC AGGACAGAAA 
GGACCACCTG GTCCTATTGG ACCTCCCGGT TTACCTGGTC TGCGTGGAGA TTCTGGCGGC 
AAAGGAGAAA AGGGACACCC GGGTCTTATC GGTCTCATTG GACCCCCAGG AGAACAGGGT 
GAGAAGGGAG ACAGAGGCAT GCCTGGGCCC CATGGATCCA ATGGACCAAA AGGAGAGACT 
GGAATGCCTG GAGGCACAGG ACCTATCGGC CCCTCTGGTC CTCCTGGTCT GCCTGGTCCT 
CAAGGTATTA AAGGAGCCAA AGGTTCTTCT GGTGGAGCTG GTCCAAAAGG AGAAAAAGGA 
GTACAAGGAC CCCCTGGACT TCCTGGCCCA CCAGGGGAGG TTATTCAGCC ACTACCCATC 
CAAAGGAGTC CCAAGTCCAA GCGGTCCATT GATGCCAGCC AGCTACTTCC TGAGAATGAC 
ATGCCTGCAT CCGATGCCAC CGGGACAGAA TTCCTGATGG GTAGTGAAGG CATGGAGGAG 
ATCTTTGGCT CCCTCAACTC GCTTCGGCAG GAGATTGAGA CCATGCGCTT CCCTCTGGGC 
ACCCAGGACA GCCCCGGCCG CACCTGCCAA GACCTGCACC TCAGTCAGCC AGAACTCAAA 
GATGGAGAAT ACTGGATTGA CCCCAACCAG GGCTGCTCCA GAGACGCCTT CAAGGTCTTC 
TGTAACTTTA CTAAGGGGGC AGAGACGTGC CTCTACCCCA GCCAGAGCAT CAGCACGGTG 
AAGATGAATT CCTGGAACAA AGAGACTCCA GGTTCCTGGT ACAGTCAGTT CTCCACTGGC 
AGTAAGTTTT CATACGTGGA CTCCAACGGC GAACCTGTGG GTGTGGTTCA GTTGGGTTTC 
CTTCGTCTCT TGAGCGTGCA GGCCCACCAG ACTCTCACAT ACCACTGCCA CCGCTCGGTA 
GCGTGGGCTG ACCGCAACGC CAAGAACACA TACAGCCGAG CTCTGCACTT CAGGGGTGCC 
AACGACGAGG ATCTAAGCTA TGAGACCAAT CCATACGTCA AGGCTTTGGT TGATGGCTGC 
TCTTATCGTA AGGGCTTTGA CCGGACAGTC CTGGAGATCA ACACACCACA GGTGGAACAT 
CTTCCTCTGC TGGATATCAA GGTGTCAGAC TTTGGGGAGA GCAACCAGCA GTTTGGCTTT 
GAAGTGGGAC CTGTCTGTTT CCAAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615671.2
CDS	408..5837
Translation	MDTGDCPCRKKRRPWRMDFSSFALVTVMLIVCQTALVRADPVDVLRALQVPSLPEGVKKVPGFCTSRRSSSPDHAYRITKKAQISAPTKQLFSGRFPENFSIMALVKAQAGLQAFLLSIYSEQGIQQLGIEMGRSPVFLYEDQNGKPAPEDYPLFRGVNLADGKWHRIALSVSKKNVTLLLDCKKKMTRPLPRSNSAEVDTNGITVFGARLLDEEVFQGDIQQLLIASNPQAAYDFCEHYSPDCDSPLPKTQAQDPNTYYFDEEQDDHEYPYYYEETTGRPSSSAPPSPQPGAPSQQDVDTEYSEAGHTPTERDYYYEETVPLPEGEVVGQEELAVQPQVEPVLEGEEVHSTKAVGEGEEAFTEEYVTGDVTLKEYDYSYRDYNEPLVETGEVDANMGPALSAVTDEGGAGVRGQKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPSGPPGSVGDPGERGPPGKAGLPGADGVPGPPGTSVMLPFRFGQSGGDKGPAVSAQEAQAAAILSQARMALKGPPGPMGFTGRPGPLGNPGSPGLKGESGDPGPQGPRGSLGLMGPPGKSGRRGRAGADGARGMPGESGSKGDRGFDGLPGLPGDKGHRGDSGPMGPPGSQGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGIPGPPGVRGSDGPHGPKGNLGPQGEPGPPGQQGTPGTQGMPGPQGHTGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGPIGYPGPRGLKGGQGIRGLKGHKGEKGEDGFPGIKGDFGIKGERGEIGVPGPRGEDGPEGPKGRFGPPGETGPLGAVGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGSNGEKGTRGLTGKSGPRGQRGPTGPRGQRGPRGATGKLGPKGTSGSDGPPGPPGERGLPGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKMGPPGPPGVVGPQGPAGETGPNGERGHPGPPGPPGEQGLPGPSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGGGGFKGSEGPAGPPGPAGSPGERGQSGTAGPVGPPGRPGPQGPPGPAGEKGVSGEKGPIGPAGRDGLQGPVGLPGPAGPPGVPGEDGDKGEVGEHGQKGAKGGKGEHGPPGPPGPMGPVGQPGAPGADGELGPRGQQGPFGAKGDEGTRGFPGAPGPIGLQGLPGPPGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGIGGEPGKKGPRGERGEKGEAGQPGTAGSTGGRGLPGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGERGEDGEAGESGSPGPPGENGPPGPLGKRGPAGTRGPEGRQGEKGTKGDPGAVGPPGKTGPVGPQGVPGKPGTEGLRGLPGSVGEQGSPGPAGQKGPPGPIGPPGLPGLRGDSGGKGEKGHPGLIGLIGPPGEQGEKGDRGMPGPHGSNGPKGETGMPGGTGPIGPSGPPGLPGPQGIKGAKGSSGGAGPKGEKGVQGPPGLPGPPGEVIQPLPIQRSPKSKRSIDASQLLPENDMPASDATGTEFLMGSEGMEEIFGSLNSLRQEIETMRFPLGTQDSPGRTCQDLHLSQPELKDGEYWIDPNQGCSRDAFKVFCNFTKGAETCLYPSQSISTVKMNSWNKETPGSWYSQFSTGSKFSYVDSNGEPVGVVQLGFLRLLSVQAHQTLTYHCHRSVAWADRNAKNTYSRALHFRGANDEDLSYETNPYVKALVDGCSYRKGFDRTVLEINTPQVEHLPLLDIKVSDFGESNQQFGFEVGPVCFQG

Target ORF information:

RefSeq Version	XM_011617369.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617369.2

ORF Insert Sequence:

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGAC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATTAC 
TTTGATGAGG AGCAGGATGA CCATGAATAC CCTTATTATT ATGAGGAAAC AACAGGCCGT 
CCCTCCTCCT CTGCACCCCC CTCTCCCCAA CCTGGGGCCC CCAGTCAACA GGATGTAGAC 
ACTGAATATT CCGAGGCTGG CCACACCCCT ACAGAGAGAG ATTATTACTA TGAGGAGACG 
GTACCACTGC CAGAGGGTGA GGTGGTTGGG CAAGAGGAAC TCGCCGTACA GCCCCAGGTG 
GAGCCTGTGT TGGAGGGAGA AGAGGTGCAT TCGACCAAGG CTGTCGGTGA GGGAGAGGAG 
GCCTTCACAG AGGAGTATGT GACTGGAGAT GTGACCCTTA AGGAATACGA CTATTCCTAC 
AGGGACTACA ATGAGCCATT AGTGGAGACT GGAGAAGTCG ATGCCAACAT GGGCCCCGCC 
CTCTCTGCTG TGACAGACGA GGGAGGCGCT GGGGTGAGAG GCCAGAAAGG AGAGAAAGGA 
GAACCTGCTG TTTTGGAGCC TGGGATGCTG ATTGAAGGAC CACCAGGACC TGAGGGACCT 
GCTGGCCTAC CTGGTCCTTC TGGCCCTTCT GGACCTCCTG GCTCTGTGGG TGACCCAGGA 
GAGAGGGGCC CTCCTGGTAA GGCTGGTCTT CCTGGAGCTG ATGGAGTCCC TGGACCTCCT 
GGGACCTCTG TTATGCTGCC TTTCCGTTTT GGTCAGAGTG GAGGAGATAA AGGTCCTGCT 
GTTTCTGCTC AGGAAGCTCA GGCAGCAGCC ATCTTGTCTC AGGCCAGGAT GGCTCTTAAA 
GGACCTCCTG GTCCAATGGG ATTCACTGGG CGCCCCGGAC CCTTGGGAAA TCCTGGAAGT 
CCTGGTCTGA AAGGAGAGAG TGGAGACCCT GGACCCCAGG GCCCGAGGGG ATCTCTAGGC 
TTGATGGGAC CTCCAGGCAA ATCAGGAAGA AGAGGCCGCG CTGGAGCCGA CGGTGCCAGA 
GGAATGCCTG GAGAGTCTGG TTCTAAGGGT GATCGTGGTT TTGATGGTCT TCCTGGTTTA 
CCTGGAGACA AAGGACACAG GGGTGACTCG GGACCAATGG GACCTCCAGG ATCTCAGGGA 
GAGGATGGAG AAAGGGGAGA CGACGGAGAC GTTGGACCCC GGGGTCTTCC AGGTGAACCA 
GGACCTCGTG GTTTGCTTGG ACCTAAAGGT CCTCCTGGAA TCCCAGGCCC TCCTGGTGTA 
CGAGGAAGTG ACGGACCCCA CGGTCCAAAA GGAAACTTGG GTCCTCAAGG TGAGCCAGGA 
CCCCCTGGTC AGCAGGGAAC CCCAGGAACT CAGGGAATGC CAGGACCTCA AGGACATACT 
GGACCTCCAG GAGAAAAGGG TCCAACAGGA AAACCGGGTT TACCAGGAAT GCCTGGAGCT 
GATGGCCCTC CTGGTCACCC AGGAAAGGAA GGCCCATCAG GCACCAAAGG AAACCAGGGT 
CCCAGTGGTC CCCAGGGGCC TATTGGCTAT CCTGGTCCTC GTGGCCTCAA GGGCGGTCAA 
GGAATCCGGG GATTGAAGGG TCACAAAGGA GAGAAGGGAG AAGATGGTTT CCCTGGAATT 
AAGGGAGACT TTGGGATCAA GGGTGAGAGG GGTGAGATTG GAGTACCAGG TCCTAGAGGA 
GAGGACGGTC CAGAGGGGCC AAAAGGTCGT TTTGGTCCCC CTGGTGAAAC TGGACCACTC 
GGAGCAGTAG GAGAGAAGGG TAAACTTGGT GTACCGGGAC TTCCTGGATA TCCTGGAAGA 
CAAGGACCAA AGGGATCTCT TGGATTCCCA GGATTCCCTG GATCCAATGG CGAGAAGGGA 
ACAAGGGGTC TCACTGGCAA ATCAGGACCA AGAGGACAAA GAGGACCAAC GGGCCCCAGA 
GGGCAGAGGG GACCGAGGGG CGCAACTGGC AAACTGGGGC CAAAGGGGAC TTCGGGAAGT 
GATGGCCCCC CTGGTCCTCC TGGAGAAAGG GGCTTGCCTG GGCCTCAGGG AGCCAATGGG 
TTCCCCGGAC CTAAAGGGCC ACCGGGGCCA CCAGGGAAGG ACGGGCTGCC TGGACACCCT 
GGGCAGAGAG GAGAAGTGGG TTTCCAAGGT AAAATGGGTC CACCTGGGCC TCCTGGAGTT 
GTTGGACCTC AGGGTCCAGC TGGAGAGACT GGCCCTAATG GTGAACGTGG CCACCCTGGA 
CCACCAGGTC CACCAGGAGA ACAGGGACTT CCTGGTCCCT CAGGAAAGGA GGGAACCAAA 
GGAGACCCTG GACCCCCAGG AGGCCCTGGC AAAGATGGGC CTCCTGGACT GAGGGGCTTC 
CCTGGTGAGA GAGGACTGCC AGGAACACCT GGAGGTGGAG GATTCAAAGG AAGCGAAGGA 
CCTGCTGGAC CTCCTGGACC GGCTGGCTCT CCTGGTGAGA GGGGGCAATC TGGTACCGCT 
GGGCCTGTTG GACCTCCTGG AAGACCAGGT CCTCAGGGAC CTCCTGGACC TGCAGGAGAG 
AAAGGTGTTT CTGGTGAGAA AGGCCCAATT GGCCCAGCTG GTCGTGACGG ACTACAGGGT 
CCTGTGGGTC TGCCTGGCCC AGCTGGACCG CCCGGAGTAC CTGGAGAAGA TGGAGATAAG 
GGGGAGGTTG GAGAACACGG TCAGAAAGGT GCCAAGGGAG GGAAAGGAGA GCATGGTCCT 
CCAGGTCCAC CTGGACCAAT GGGACCAGTC GGCCAGCCTG GTGCTCCAGG TGCTGATGGA 
GAGCTTGGGC CGAGGGGTCA GCAGGGACCT TTTGGAGCTA AAGGGGATGA AGGAACCAGA 
GGATTCCCAG GAGCCCCAGG ACCCATTGGA CTCCAGGGTT TGCCTGGGCC ACCAGGTGAG 
AAGGGAGAAA CCGGAGATGT TGGACCTCTG GGACCTCCAG GCCCTCCAGG ACCCCGTGGC 
CCTGCTGGAC CCAACGGTGC TGATGGCCCT CAAGGGCCTC CTGGTGGTTT GGGTAATCCT 
GGACCTATCG GTGAGAAGGG AGAGCCTGGA GAGTCTGGAC CCCCTGGAAT TGGAGGGGAG 
CCTGGAAAGA AGGGCCCCAG AGGGGAACGT GGGGAGAAGG GGGAGGCTGG ACAACCAGGA 
ACCGCTGGAT CCACTGGAGG ACGTGGACTA CCTGGAGATG ATGGACCCAA AGGAAACCCA 
GGTCCTGTTG GTTTCCCCGG TGATCCTGGT CCACCGGGTG AGGTTGGACC CAGAGGTCAA 
GACGGTGCCA AGGGAGAAAG AGGAGAAGAT GGTGAAGCAG GAGAGTCTGG ATCCCCTGGA 
CCTCCCGGTG AGAACGGACC CCCCGGCCCC CTAGGAAAGA GGGGTCCTGC TGGAACAAGA 
GGACCAGAGG GACGCCAGGG AGAGAAGGGA ACAAAGGGGG ATCCAGGTGC TGTTGGCCCC 
CCAGGCAAAA CAGGCCCTGT GGGTCCACAG GGAGTGCCAG GAAAACCTGG AACAGAAGGC 
CTTAGAGGGC TTCCAGGATC AGTGGGTGAG CAAGGATCTC CAGGACCTGC AGGACAGAAA 
GGACCACCTG GTCCTATTGG ACCTCCCGGT TTACCTGGTC TGCGTGGAGA TTCTGGCGGC 
AAAGGAGAAA AGGGACACCC GGGTCTTATC GGTCTCATTG GACCCCCAGG AGAACAGGGT 
GAGAAGGGAG ACAGAGGCAT GCCTGGGCCC CATGGATCCA ATGGACCAAA AGGAGAGACT 
GGAATGCCTG GAGGCACAGG ACCTATCGGC CCCTCTGGTC CTCCTGGTCT GCCTGGTCCT 
CAAGGTATTA AAGGAGCCAA AGGTTCTTCT GGTGGAGCTG GTCCAAAAGG AGAAAAAGGA 
GTACAAGGAC CCCCTGGACT TCCTGGCCCA CCAGGGGAGG TTATTCAGCC ACTACCCATC 
CAAAGGAGTC CCAAGTCCAA GCGGTCCATT GATGCCAGCC AGCTACTTCC TGAGAATGAC 
ATGCCTGCAT CCGATGCCAC CGGGACAGAA TTCCTGATGG GTAGTGAAGG CATGGAGGAG 
ATCTTTGGCT CCCTCAACTC GCTTCGGCAG GAGATTGAGA CCATGCGCTT CCCTCTGGGC 
ACCCAGGACA GCCCCGGCCG CACCTGCCAA GACCTGCACC TCAGTCAGCC AGAACTCAAA 
GATGGAGAAT ACTGGATTGA CCCCAACCAG GGCTGCTCCA GAGACGCCTT CAAGGTCTTC 
TGTAACTTTA CTAAGGGGGC AGAGACGTGC CTCTACCCCA GCCAGAGCAT CAGCACGGTG 
AAGATGAATT CCTGGAACAA AGAGACTCCA GGTTCCTGGT ACAGTCAGTT CTCCACTGGC 
AGTAAGTTTT CATACGTGGA CTCCAACGGC GAACCTGTGG GTGTGGTTCA GTTGGGTTTC 
CTTCGTCTCT TGAGCGTGCA GGCCCACCAG ACTCTCACAT ACCACTGCCA CCGCTCGGTA 
GCGTGGGCTG ACCGCAACGC CAAGAACACA TACAGCCGAG CTCTGCACTT CAGGGGTGCC 
AACGACGAGG ATCTAAGCTA TGAGACCAAT CCATACGTCA AGGCTTTGGT TGATGGCTGC 
TCTTATCGTA AGGGCTTTGA CCGGACAGTC CTGGAGATCA ACACACCACA GGTGGAACAT 
CTTCCTCTGC TGGATATCAA GGTGTCAGAC TTTGGGGAGA GCAACCAGCA GTTTGGCTTT 
GAAGTGGGAC CTGTCTGTTT CCAAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101066527 ( XM_011617370.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011615672.2 Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X5, mRNA.

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CloneID	OTo45741
Clone ID Related Accession (Same CDS sequence)	XM_011617370.2
Accession Version	XM_011617370.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	collagen alpha-2(XI) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042296.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011617370.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGAC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATGAT 
GTAGACACTG AATATTCCGA GGCTGGCCAC ACCCCTACAG AGAGAGATTA TTACTATGAG 
GAGACGGTAC CACTGCCAGA GGGTGAGGTG GTTGGGCAAG AGGAACTCGC CGTACAGCCC 
CAGGTGGAGC CTGTGTTGGA GGGAGAAGAG GTGCATTCGA CCAAGGCTGT CGGTGAGGGA 
GAGGAGGCCT TCACAGAGGA GTATGTGACT GGAGATGTGA CCCTTAAGGA ATACGACTAT 
TCCTACAGGG ACTACAATGA GCCATTAGTG GAGACTGGAG AAGTCGATGC CAACATGGGC 
CCCGCCCTCT CTGCTGTGAC AGACGAGGGA GGCGCTGGGG TGAGAGGCCA GAAAGGAGAG 
AAAGGAGAAC CTGCTGTTTT GGAGCCTGGG ATGCTGATTG AAGGACCACC AGGACCTGAG 
GGACCTGCTG GCCTACCTGG TCCTTCTGGC CCTTCTGGAC CTCCTGGCTC TGTGGGTGAC 
CCAGGAGAGA GGGGCCCTCC TGGTAAGGCT GGTCTTCCTG GAGCTGATGG AGTCCCTGGA 
CCTCCTGGGA CCTCTGTTAT GCTGCCTTTC CGTTTTGGTC AGAGTGGAGG AGATAAAGGT 
CCTGCTGTTT CTGCTCAGGA AGCTCAGGCA GCAGCCATCT TGTCTCAGGC CAGGATGGCT 
CTTAAAGGAC CTCCTGGTCC AATGGGATTC ACTGGGCGCC CCGGACCCTT GGGAAATCCT 
GGAAGTCCTG GTCTGAAAGG AGAGAGTGGA GACCCTGGAC CCCAGGGCCC GAGGGGATCT 
CTAGGCTTGA TGGGACCTCC AGGCAAATCA GGAAGAAGAG GCCGCGCTGG AGCCGACGGT 
GCCAGAGGAA TGCCTGGAGA GTCTGGTTCT AAGGGTGATC GTGGTTTTGA TGGTCTTCCT 
GGTTTACCTG GAGACAAAGG ACACAGGGGT GACTCGGGAC CAATGGGACC TCCAGGATCT 
CAGGGAGAGG ATGGAGAAAG GGGAGACGAC GGAGACGTTG GACCCCGGGG TCTTCCAGGT 
GAACCAGGAC CTCGTGGTTT GCTTGGACCT AAAGGTCCTC CTGGAATCCC AGGCCCTCCT 
GGTGTACGAG GAAGTGACGG ACCCCACGGT CCAAAAGGAA ACTTGGGTCC TCAAGGTGAG 
CCAGGACCCC CTGGTCAGCA GGGAACCCCA GGAACTCAGG GAATGCCAGG ACCTCAAGGA 
CATACTGGAC CTCCAGGAGA AAAGGGTCCA ACAGGAAAAC CGGGTTTACC AGGAATGCCT 
GGAGCTGATG GCCCTCCTGG TCACCCAGGA AAGGAAGGCC CATCAGGCAC CAAAGGAAAC 
CAGGGTCCCA GTGGTCCCCA GGGGCCTATT GGCTATCCTG GTCCTCGTGG CCTCAAGGGC 
GGTCAAGGAA TCCGGGGATT GAAGGGTCAC AAAGGAGAGA AGGGAGAAGA TGGTTTCCCT 
GGAATTAAGG GAGACTTTGG GATCAAGGGT GAGAGGGGTG AGATTGGAGT ACCAGGTCCT 
AGAGGAGAGG ACGGTCCAGA GGGGCCAAAA GGTCGTTTTG GTCCCCCTGG TGAAACTGGA 
CCACTCGGAG CAGTAGGAGA GAAGGGTAAA CTTGGTGTAC CGGGACTTCC TGGATATCCT 
GGAAGACAAG GACCAAAGGG ATCTCTTGGA TTCCCAGGAT TCCCTGGATC CAATGGCGAG 
AAGGGAACAA GGGGTCTCAC TGGCAAATCA GGACCAAGAG GACAAAGAGG ACCAACGGGC 
CCCAGAGGGC AGAGGGGACC GAGGGGCGCA ACTGGCAAAC TGGGGCCAAA GGGGACTTCG 
GGAAGTGATG GCCCCCCTGG TCCTCCTGGA GAAAGGGGCT TGCCTGGGCC TCAGGGAGCC 
AATGGGTTCC CCGGACCTAA AGGGCCACCG GGGCCACCAG GGAAGGACGG GCTGCCTGGA 
CACCCTGGGC AGAGAGGAGA AGTGGGTTTC CAAGGTAAAA TGGGTCCACC TGGGCCTCCT 
GGAGTTGTTG GACCTCAGGG TCCAGCTGGA GAGACTGGCC CTAATGGTGA ACGTGGCCAC 
CCTGGACCAC CAGGTCCACC AGGAGAACAG GGACTTCCTG GTCCCTCAGG AAAGGAGGGA 
ACCAAAGGAG ACCCTGGACC CCCAGGAGGC CCTGGCAAAG ATGGGCCTCC TGGACTGAGG 
GGCTTCCCTG GTGAGAGAGG ACTGCCAGGA ACACCTGGAG GTGGAGGATT CAAAGGAAGC 
GAAGGACCTG CTGGACCTCC TGGACCGGCT GGCTCTCCTG GTGAGAGGGG GCAATCTGGT 
ACCGCTGGGC CTGTTGGACC TCCTGGAAGA CCAGGTCCTC AGGGACCTCC TGGACCTGCA 
GGAGAGAAAG GTGTTTCTGG TGAGAAAGGC CCAATTGGCC CAGCTGGTCG TGACGGACTA 
CAGGGTCCTG TGGGTCTGCC TGGCCCAGCT GGACCGCCCG GAGTACCTGG AGAAGATGGA 
GATAAGGGGG AGGTTGGAGA ACACGGTCAG AAAGGTGCCA AGGGAGGGAA AGGAGAGCAT 
GGTCCTCCAG GTCCACCTGG ACCAATGGGA CCAGTCGGCC AGCCTGGTGC TCCAGGTGCT 
GATGGAGAGC TTGGGCCGAG GGGTCAGCAG GGACCTTTTG GAGCTAAAGG GGATGAAGGA 
ACCAGAGGAT TCCCAGGAGC CCCAGGACCC ATTGGACTCC AGGGTTTGCC TGGGCCACCA 
GGTGAGAAGG GAGAAACCGG AGATGTTGGA CCTCTGGGAC CTCCAGGCCC TCCAGGACCC 
CGTGGCCCTG CTGGACCCAA CGGTGCTGAT GGCCCTCAAG GGCCTCCTGG TGGTTTGGGT 
AATCCTGGAC CTATCGGTGA GAAGGGAGAG CCTGGAGAGT CTGGACCCCC TGGAATTGGA 
GGGGAGCCTG GAAAGAAGGG CCCCAGAGGG GAACGTGGGG AGAAGGGGGA GGCTGGACAA 
CCAGGAACCG CTGGATCCAC TGGAGGACGT GGACTACCTG GAGATGATGG ACCCAAAGGA 
AACCCAGGTC CTGTTGGTTT CCCCGGTGAT CCTGGTCCAC CGGGTGAGGT TGGACCCAGA 
GGTCAAGACG GTGCCAAGGG AGAAAGAGGA GAAGATGGTG AAGCAGGAGA GTCTGGATCC 
CCTGGACCTC CCGGTGAGAA CGGACCCCCC GGCCCCCTAG GAAAGAGGGG TCCTGCTGGA 
ACAAGAGGAC CAGAGGGACG CCAGGGAGAG AAGGGAACAA AGGGGGATCC AGGTGCTGTT 
GGCCCCCCAG GCAAAACAGG CCCTGTGGGT CCACAGGGAG TGCCAGGAAA ACCTGGAACA 
GAAGGCCTTA GAGGGCTTCC AGGATCAGTG GGTGAGCAAG GATCTCCAGG ACCTGCAGGA 
CAGAAAGGAC CACCTGGTCC TATTGGACCT CCCGGTTTAC CTGGTCTGCG TGGAGATTCT 
GGCGGCAAAG GAGAAAAGGG ACACCCGGGT CTTATCGGTC TCATTGGACC CCCAGGAGAA 
CAGGGTGAGA AGGGAGACAG AGGCATGCCT GGGCCCCATG GATCCAATGG ACCAAAAGGA 
GAGACTGGAA TGCCTGGAGG CACAGGACCT ATCGGCCCCT CTGGTCCTCC TGGTCTGCCT 
GGTCCTCAAG GTATTAAAGG AGCCAAAGGT TCTTCTGGTG GAGCTGGTCC AAAAGGAGAA 
AAAGGAGTAC AAGGACCCCC TGGACTTCCT GGCCCACCAG GGGAGGTTAT TCAGCCACTA 
CCCATCCAAA GGAGTCCCAA GTCCAAGCGG TCCATTGATG CCAGCCAGCT ACTTCCTGAG 
AATGACATGC CTGCATCCGA TGCCACCGGG ACAGAATTCC TGATGGGTAG TGAAGGCATG 
GAGGAGATCT TTGGCTCCCT CAACTCGCTT CGGCAGGAGA TTGAGACCAT GCGCTTCCCT 
CTGGGCACCC AGGACAGCCC CGGCCGCACC TGCCAAGACC TGCACCTCAG TCAGCCAGAA 
CTCAAAGATG GAGAATACTG GATTGACCCC AACCAGGGCT GCTCCAGAGA CGCCTTCAAG 
GTCTTCTGTA ACTTTACTAA GGGGGCAGAG ACGTGCCTCT ACCCCAGCCA GAGCATCAGC 
ACGGTGAAGA TGAATTCCTG GAACAAAGAG ACTCCAGGTT CCTGGTACAG TCAGTTCTCC 
ACTGGCAGTA AGTTTTCATA CGTGGACTCC AACGGCGAAC CTGTGGGTGT GGTTCAGTTG 
GGTTTCCTTC GTCTCTTGAG CGTGCAGGCC CACCAGACTC TCACATACCA CTGCCACCGC 
TCGGTAGCGT GGGCTGACCG CAACGCCAAG AACACATACA GCCGAGCTCT GCACTTCAGG 
GGTGCCAACG ACGAGGATCT AAGCTATGAG ACCAATCCAT ACGTCAAGGC TTTGGTTGAT 
GGCTGCTCTT ATCGTAAGGG CTTTGACCGG ACAGTCCTGG AGATCAACAC ACCACAGGTG 
GAACATCTTC CTCTGCTGGA TATCAAGGTG TCAGACTTTG GGGAGAGCAA CCAGCAGTTT 
GGCTTTGAAG TGGGACCTGT CTGTTTCCAA GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615672.2
CDS	408..5723
Translation	MDTGDCPCRKKRRPWRMDFSSFALVTVMLIVCQTALVRADPVDVLRALQVPSLPEGVKKVPGFCTSRRSSSPDHAYRITKKAQISAPTKQLFSGRFPENFSIMALVKAQAGLQAFLLSIYSEQGIQQLGIEMGRSPVFLYEDQNGKPAPEDYPLFRGVNLADGKWHRIALSVSKKNVTLLLDCKKKMTRPLPRSNSAEVDTNGITVFGARLLDEEVFQGDIQQLLIASNPQAAYDFCEHYSPDCDSPLPKTQAQDPNTYDVDTEYSEAGHTPTERDYYYEETVPLPEGEVVGQEELAVQPQVEPVLEGEEVHSTKAVGEGEEAFTEEYVTGDVTLKEYDYSYRDYNEPLVETGEVDANMGPALSAVTDEGGAGVRGQKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPSGPPGSVGDPGERGPPGKAGLPGADGVPGPPGTSVMLPFRFGQSGGDKGPAVSAQEAQAAAILSQARMALKGPPGPMGFTGRPGPLGNPGSPGLKGESGDPGPQGPRGSLGLMGPPGKSGRRGRAGADGARGMPGESGSKGDRGFDGLPGLPGDKGHRGDSGPMGPPGSQGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGIPGPPGVRGSDGPHGPKGNLGPQGEPGPPGQQGTPGTQGMPGPQGHTGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGPIGYPGPRGLKGGQGIRGLKGHKGEKGEDGFPGIKGDFGIKGERGEIGVPGPRGEDGPEGPKGRFGPPGETGPLGAVGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGSNGEKGTRGLTGKSGPRGQRGPTGPRGQRGPRGATGKLGPKGTSGSDGPPGPPGERGLPGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKMGPPGPPGVVGPQGPAGETGPNGERGHPGPPGPPGEQGLPGPSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGGGGFKGSEGPAGPPGPAGSPGERGQSGTAGPVGPPGRPGPQGPPGPAGEKGVSGEKGPIGPAGRDGLQGPVGLPGPAGPPGVPGEDGDKGEVGEHGQKGAKGGKGEHGPPGPPGPMGPVGQPGAPGADGELGPRGQQGPFGAKGDEGTRGFPGAPGPIGLQGLPGPPGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGIGGEPGKKGPRGERGEKGEAGQPGTAGSTGGRGLPGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGERGEDGEAGESGSPGPPGENGPPGPLGKRGPAGTRGPEGRQGEKGTKGDPGAVGPPGKTGPVGPQGVPGKPGTEGLRGLPGSVGEQGSPGPAGQKGPPGPIGPPGLPGLRGDSGGKGEKGHPGLIGLIGPPGEQGEKGDRGMPGPHGSNGPKGETGMPGGTGPIGPSGPPGLPGPQGIKGAKGSSGGAGPKGEKGVQGPPGLPGPPGEVIQPLPIQRSPKSKRSIDASQLLPENDMPASDATGTEFLMGSEGMEEIFGSLNSLRQEIETMRFPLGTQDSPGRTCQDLHLSQPELKDGEYWIDPNQGCSRDAFKVFCNFTKGAETCLYPSQSISTVKMNSWNKETPGSWYSQFSTGSKFSYVDSNGEPVGVVQLGFLRLLSVQAHQTLTYHCHRSVAWADRNAKNTYSRALHFRGANDEDLSYETNPYVKALVDGCSYRKGFDRTVLEINTPQVEHLPLLDIKVSDFGESNQQFGFEVGPVCFQG

Target ORF information:

RefSeq Version	XM_011617370.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617370.2

ORF Insert Sequence:

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGAC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATGAT 
GTAGACACTG AATATTCCGA GGCTGGCCAC ACCCCTACAG AGAGAGATTA TTACTATGAG 
GAGACGGTAC CACTGCCAGA GGGTGAGGTG GTTGGGCAAG AGGAACTCGC CGTACAGCCC 
CAGGTGGAGC CTGTGTTGGA GGGAGAAGAG GTGCATTCGA CCAAGGCTGT CGGTGAGGGA 
GAGGAGGCCT TCACAGAGGA GTATGTGACT GGAGATGTGA CCCTTAAGGA ATACGACTAT 
TCCTACAGGG ACTACAATGA GCCATTAGTG GAGACTGGAG AAGTCGATGC CAACATGGGC 
CCCGCCCTCT CTGCTGTGAC AGACGAGGGA GGCGCTGGGG TGAGAGGCCA GAAAGGAGAG 
AAAGGAGAAC CTGCTGTTTT GGAGCCTGGG ATGCTGATTG AAGGACCACC AGGACCTGAG 
GGACCTGCTG GCCTACCTGG TCCTTCTGGC CCTTCTGGAC CTCCTGGCTC TGTGGGTGAC 
CCAGGAGAGA GGGGCCCTCC TGGTAAGGCT GGTCTTCCTG GAGCTGATGG AGTCCCTGGA 
CCTCCTGGGA CCTCTGTTAT GCTGCCTTTC CGTTTTGGTC AGAGTGGAGG AGATAAAGGT 
CCTGCTGTTT CTGCTCAGGA AGCTCAGGCA GCAGCCATCT TGTCTCAGGC CAGGATGGCT 
CTTAAAGGAC CTCCTGGTCC AATGGGATTC ACTGGGCGCC CCGGACCCTT GGGAAATCCT 
GGAAGTCCTG GTCTGAAAGG AGAGAGTGGA GACCCTGGAC CCCAGGGCCC GAGGGGATCT 
CTAGGCTTGA TGGGACCTCC AGGCAAATCA GGAAGAAGAG GCCGCGCTGG AGCCGACGGT 
GCCAGAGGAA TGCCTGGAGA GTCTGGTTCT AAGGGTGATC GTGGTTTTGA TGGTCTTCCT 
GGTTTACCTG GAGACAAAGG ACACAGGGGT GACTCGGGAC CAATGGGACC TCCAGGATCT 
CAGGGAGAGG ATGGAGAAAG GGGAGACGAC GGAGACGTTG GACCCCGGGG TCTTCCAGGT 
GAACCAGGAC CTCGTGGTTT GCTTGGACCT AAAGGTCCTC CTGGAATCCC AGGCCCTCCT 
GGTGTACGAG GAAGTGACGG ACCCCACGGT CCAAAAGGAA ACTTGGGTCC TCAAGGTGAG 
CCAGGACCCC CTGGTCAGCA GGGAACCCCA GGAACTCAGG GAATGCCAGG ACCTCAAGGA 
CATACTGGAC CTCCAGGAGA AAAGGGTCCA ACAGGAAAAC CGGGTTTACC AGGAATGCCT 
GGAGCTGATG GCCCTCCTGG TCACCCAGGA AAGGAAGGCC CATCAGGCAC CAAAGGAAAC 
CAGGGTCCCA GTGGTCCCCA GGGGCCTATT GGCTATCCTG GTCCTCGTGG CCTCAAGGGC 
GGTCAAGGAA TCCGGGGATT GAAGGGTCAC AAAGGAGAGA AGGGAGAAGA TGGTTTCCCT 
GGAATTAAGG GAGACTTTGG GATCAAGGGT GAGAGGGGTG AGATTGGAGT ACCAGGTCCT 
AGAGGAGAGG ACGGTCCAGA GGGGCCAAAA GGTCGTTTTG GTCCCCCTGG TGAAACTGGA 
CCACTCGGAG CAGTAGGAGA GAAGGGTAAA CTTGGTGTAC CGGGACTTCC TGGATATCCT 
GGAAGACAAG GACCAAAGGG ATCTCTTGGA TTCCCAGGAT TCCCTGGATC CAATGGCGAG 
AAGGGAACAA GGGGTCTCAC TGGCAAATCA GGACCAAGAG GACAAAGAGG ACCAACGGGC 
CCCAGAGGGC AGAGGGGACC GAGGGGCGCA ACTGGCAAAC TGGGGCCAAA GGGGACTTCG 
GGAAGTGATG GCCCCCCTGG TCCTCCTGGA GAAAGGGGCT TGCCTGGGCC TCAGGGAGCC 
AATGGGTTCC CCGGACCTAA AGGGCCACCG GGGCCACCAG GGAAGGACGG GCTGCCTGGA 
CACCCTGGGC AGAGAGGAGA AGTGGGTTTC CAAGGTAAAA TGGGTCCACC TGGGCCTCCT 
GGAGTTGTTG GACCTCAGGG TCCAGCTGGA GAGACTGGCC CTAATGGTGA ACGTGGCCAC 
CCTGGACCAC CAGGTCCACC AGGAGAACAG GGACTTCCTG GTCCCTCAGG AAAGGAGGGA 
ACCAAAGGAG ACCCTGGACC CCCAGGAGGC CCTGGCAAAG ATGGGCCTCC TGGACTGAGG 
GGCTTCCCTG GTGAGAGAGG ACTGCCAGGA ACACCTGGAG GTGGAGGATT CAAAGGAAGC 
GAAGGACCTG CTGGACCTCC TGGACCGGCT GGCTCTCCTG GTGAGAGGGG GCAATCTGGT 
ACCGCTGGGC CTGTTGGACC TCCTGGAAGA CCAGGTCCTC AGGGACCTCC TGGACCTGCA 
GGAGAGAAAG GTGTTTCTGG TGAGAAAGGC CCAATTGGCC CAGCTGGTCG TGACGGACTA 
CAGGGTCCTG TGGGTCTGCC TGGCCCAGCT GGACCGCCCG GAGTACCTGG AGAAGATGGA 
GATAAGGGGG AGGTTGGAGA ACACGGTCAG AAAGGTGCCA AGGGAGGGAA AGGAGAGCAT 
GGTCCTCCAG GTCCACCTGG ACCAATGGGA CCAGTCGGCC AGCCTGGTGC TCCAGGTGCT 
GATGGAGAGC TTGGGCCGAG GGGTCAGCAG GGACCTTTTG GAGCTAAAGG GGATGAAGGA 
ACCAGAGGAT TCCCAGGAGC CCCAGGACCC ATTGGACTCC AGGGTTTGCC TGGGCCACCA 
GGTGAGAAGG GAGAAACCGG AGATGTTGGA CCTCTGGGAC CTCCAGGCCC TCCAGGACCC 
CGTGGCCCTG CTGGACCCAA CGGTGCTGAT GGCCCTCAAG GGCCTCCTGG TGGTTTGGGT 
AATCCTGGAC CTATCGGTGA GAAGGGAGAG CCTGGAGAGT CTGGACCCCC TGGAATTGGA 
GGGGAGCCTG GAAAGAAGGG CCCCAGAGGG GAACGTGGGG AGAAGGGGGA GGCTGGACAA 
CCAGGAACCG CTGGATCCAC TGGAGGACGT GGACTACCTG GAGATGATGG ACCCAAAGGA 
AACCCAGGTC CTGTTGGTTT CCCCGGTGAT CCTGGTCCAC CGGGTGAGGT TGGACCCAGA 
GGTCAAGACG GTGCCAAGGG AGAAAGAGGA GAAGATGGTG AAGCAGGAGA GTCTGGATCC 
CCTGGACCTC CCGGTGAGAA CGGACCCCCC GGCCCCCTAG GAAAGAGGGG TCCTGCTGGA 
ACAAGAGGAC CAGAGGGACG CCAGGGAGAG AAGGGAACAA AGGGGGATCC AGGTGCTGTT 
GGCCCCCCAG GCAAAACAGG CCCTGTGGGT CCACAGGGAG TGCCAGGAAA ACCTGGAACA 
GAAGGCCTTA GAGGGCTTCC AGGATCAGTG GGTGAGCAAG GATCTCCAGG ACCTGCAGGA 
CAGAAAGGAC CACCTGGTCC TATTGGACCT CCCGGTTTAC CTGGTCTGCG TGGAGATTCT 
GGCGGCAAAG GAGAAAAGGG ACACCCGGGT CTTATCGGTC TCATTGGACC CCCAGGAGAA 
CAGGGTGAGA AGGGAGACAG AGGCATGCCT GGGCCCCATG GATCCAATGG ACCAAAAGGA 
GAGACTGGAA TGCCTGGAGG CACAGGACCT ATCGGCCCCT CTGGTCCTCC TGGTCTGCCT 
GGTCCTCAAG GTATTAAAGG AGCCAAAGGT TCTTCTGGTG GAGCTGGTCC AAAAGGAGAA 
AAAGGAGTAC AAGGACCCCC TGGACTTCCT GGCCCACCAG GGGAGGTTAT TCAGCCACTA 
CCCATCCAAA GGAGTCCCAA GTCCAAGCGG TCCATTGATG CCAGCCAGCT ACTTCCTGAG 
AATGACATGC CTGCATCCGA TGCCACCGGG ACAGAATTCC TGATGGGTAG TGAAGGCATG 
GAGGAGATCT TTGGCTCCCT CAACTCGCTT CGGCAGGAGA TTGAGACCAT GCGCTTCCCT 
CTGGGCACCC AGGACAGCCC CGGCCGCACC TGCCAAGACC TGCACCTCAG TCAGCCAGAA 
CTCAAAGATG GAGAATACTG GATTGACCCC AACCAGGGCT GCTCCAGAGA CGCCTTCAAG 
GTCTTCTGTA ACTTTACTAA GGGGGCAGAG ACGTGCCTCT ACCCCAGCCA GAGCATCAGC 
ACGGTGAAGA TGAATTCCTG GAACAAAGAG ACTCCAGGTT CCTGGTACAG TCAGTTCTCC 
ACTGGCAGTA AGTTTTCATA CGTGGACTCC AACGGCGAAC CTGTGGGTGT GGTTCAGTTG 
GGTTTCCTTC GTCTCTTGAG CGTGCAGGCC CACCAGACTC TCACATACCA CTGCCACCGC 
TCGGTAGCGT GGGCTGACCG CAACGCCAAG AACACATACA GCCGAGCTCT GCACTTCAGG 
GGTGCCAACG ACGAGGATCT AAGCTATGAG ACCAATCCAT ACGTCAAGGC TTTGGTTGAT 
GGCTGCTCTT ATCGTAAGGG CTTTGACCGG ACAGTCCTGG AGATCAACAC ACCACAGGTG 
GAACATCTTC CTCTGCTGGA TATCAAGGTG TCAGACTTTG GGGAGAGCAA CCAGCAGTTT 
GGCTTTGAAG TGGGACCTGT CTGTTTCCAA GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101066527 ( XM_011617371.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011615673.2 Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X6, mRNA.

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CloneID	OTo45742
Clone ID Related Accession (Same CDS sequence)	XM_011617371.2
Accession Version	XM_011617371.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	collagen alpha-2(XI) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042296.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011617371.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGAC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATCCC 
CAGGTGGAGC CTGTGTTGGA GGGAGAAGAG GTGCATTCGA CCAAGGCTGT CGGTGAGGGA 
GAGGAGGCCT TCACAGAGGA GTATGTGACT GGAGATGTGA CCCTTAAGGA ATACGACTAT 
TCCTACAGGG ACTACAATGA GCCATTAGTG GAGACTGGAG AAGTCGATGC CAACATGGGC 
CCCGCCCTCT CTGCTGTGAC AGACGAGGGA GGCGCTGGGG TGAGAGGCCA GAAAGGAGAG 
AAAGGAGAAC CTGCTGTTTT GGAGCCTGGG ATGCTGATTG AAGGACCACC AGGACCTGAG 
GGACCTGCTG GCCTACCTGG TCCTTCTGGC CCTTCTGGAC CTCCTGGCTC TGTGGGTGAC 
CCAGGAGAGA GGGGCCCTCC TGGTAAGGCT GGTCTTCCTG GAGCTGATGG AGTCCCTGGA 
CCTCCTGGGA CCTCTGTTAT GCTGCCTTTC CGTTTTGGTC AGAGTGGAGG AGATAAAGGT 
CCTGCTGTTT CTGCTCAGGA AGCTCAGGCA GCAGCCATCT TGTCTCAGGC CAGGATGGCT 
CTTAAAGGAC CTCCTGGTCC AATGGGATTC ACTGGGCGCC CCGGACCCTT GGGAAATCCT 
GGAAGTCCTG GTCTGAAAGG AGAGAGTGGA GACCCTGGAC CCCAGGGCCC GAGGGGATCT 
CTAGGCTTGA TGGGACCTCC AGGCAAATCA GGAAGAAGAG GCCGCGCTGG AGCCGACGGT 
GCCAGAGGAA TGCCTGGAGA GTCTGGTTCT AAGGGTGATC GTGGTTTTGA TGGTCTTCCT 
GGTTTACCTG GAGACAAAGG ACACAGGGGT GACTCGGGAC CAATGGGACC TCCAGGATCT 
CAGGGAGAGG ATGGAGAAAG GGGAGACGAC GGAGACGTTG GACCCCGGGG TCTTCCAGGT 
GAACCAGGAC CTCGTGGTTT GCTTGGACCT AAAGGTCCTC CTGGAATCCC AGGCCCTCCT 
GGTGTACGAG GAAGTGACGG ACCCCACGGT CCAAAAGGAA ACTTGGGTCC TCAAGGTGAG 
CCAGGACCCC CTGGTCAGCA GGGAACCCCA GGAACTCAGG GAATGCCAGG ACCTCAAGGA 
CATACTGGAC CTCCAGGAGA AAAGGGTCCA ACAGGAAAAC CGGGTTTACC AGGAATGCCT 
GGAGCTGATG GCCCTCCTGG TCACCCAGGA AAGGAAGGCC CATCAGGCAC CAAAGGAAAC 
CAGGGTCCCA GTGGTCCCCA GGGGCCTATT GGCTATCCTG GTCCTCGTGG CCTCAAGGGC 
GGTCAAGGAA TCCGGGGATT GAAGGGTCAC AAAGGAGAGA AGGGAGAAGA TGGTTTCCCT 
GGAATTAAGG GAGACTTTGG GATCAAGGGT GAGAGGGGTG AGATTGGAGT ACCAGGTCCT 
AGAGGAGAGG ACGGTCCAGA GGGGCCAAAA GGTCGTTTTG GTCCCCCTGG TGAAACTGGA 
CCACTCGGAG CAGTAGGAGA GAAGGGTAAA CTTGGTGTAC CGGGACTTCC TGGATATCCT 
GGAAGACAAG GACCAAAGGG ATCTCTTGGA TTCCCAGGAT TCCCTGGATC CAATGGCGAG 
AAGGGAACAA GGGGTCTCAC TGGCAAATCA GGACCAAGAG GACAAAGAGG ACCAACGGGC 
CCCAGAGGGC AGAGGGGACC GAGGGGCGCA ACTGGCAAAC TGGGGCCAAA GGGGACTTCG 
GGAAGTGATG GCCCCCCTGG TCCTCCTGGA GAAAGGGGCT TGCCTGGGCC TCAGGGAGCC 
AATGGGTTCC CCGGACCTAA AGGGCCACCG GGGCCACCAG GGAAGGACGG GCTGCCTGGA 
CACCCTGGGC AGAGAGGAGA AGTGGGTTTC CAAGGTAAAA TGGGTCCACC TGGGCCTCCT 
GGAGTTGTTG GACCTCAGGG TCCAGCTGGA GAGACTGGCC CTAATGGTGA ACGTGGCCAC 
CCTGGACCAC CAGGTCCACC AGGAGAACAG GGACTTCCTG GTCCCTCAGG AAAGGAGGGA 
ACCAAAGGAG ACCCTGGACC CCCAGGAGGC CCTGGCAAAG ATGGGCCTCC TGGACTGAGG 
GGCTTCCCTG GTGAGAGAGG ACTGCCAGGA ACACCTGGAG GTGGAGGATT CAAAGGAAGC 
GAAGGACCTG CTGGACCTCC TGGACCGGCT GGCTCTCCTG GTGAGAGGGG GCAATCTGGT 
ACCGCTGGGC CTGTTGGACC TCCTGGAAGA CCAGGTCCTC AGGGACCTCC TGGACCTGCA 
GGAGAGAAAG GTGTTTCTGG TGAGAAAGGC CCAATTGGCC CAGCTGGTCG TGACGGACTA 
CAGGGTCCTG TGGGTCTGCC TGGCCCAGCT GGACCGCCCG GAGTACCTGG AGAAGATGGA 
GATAAGGGGG AGGTTGGAGA ACACGGTCAG AAAGGTGCCA AGGGAGGGAA AGGAGAGCAT 
GGTCCTCCAG GTCCACCTGG ACCAATGGGA CCAGTCGGCC AGCCTGGTGC TCCAGGTGCT 
GATGGAGAGC TTGGGCCGAG GGGTCAGCAG GGACCTTTTG GAGCTAAAGG GGATGAAGGA 
ACCAGAGGAT TCCCAGGAGC CCCAGGACCC ATTGGACTCC AGGGTTTGCC TGGGCCACCA 
GGTGAGAAGG GAGAAACCGG AGATGTTGGA CCTCTGGGAC CTCCAGGCCC TCCAGGACCC 
CGTGGCCCTG CTGGACCCAA CGGTGCTGAT GGCCCTCAAG GGCCTCCTGG TGGTTTGGGT 
AATCCTGGAC CTATCGGTGA GAAGGGAGAG CCTGGAGAGT CTGGACCCCC TGGAATTGGA 
GGGGAGCCTG GAAAGAAGGG CCCCAGAGGG GAACGTGGGG AGAAGGGGGA GGCTGGACAA 
CCAGGAACCG CTGGATCCAC TGGAGGACGT GGACTACCTG GAGATGATGG ACCCAAAGGA 
AACCCAGGTC CTGTTGGTTT CCCCGGTGAT CCTGGTCCAC CGGGTGAGGT TGGACCCAGA 
GGTCAAGACG GTGCCAAGGG AGAAAGAGGA GAAGATGGTG AAGCAGGAGA GTCTGGATCC 
CCTGGACCTC CCGGTGAGAA CGGACCCCCC GGCCCCCTAG GAAAGAGGGG TCCTGCTGGA 
ACAAGAGGAC CAGAGGGACG CCAGGGAGAG AAGGGAACAA AGGGGGATCC AGGTGCTGTT 
GGCCCCCCAG GCAAAACAGG CCCTGTGGGT CCACAGGGAG TGCCAGGAAA ACCTGGAACA 
GAAGGCCTTA GAGGGCTTCC AGGATCAGTG GGTGAGCAAG GATCTCCAGG ACCTGCAGGA 
CAGAAAGGAC CACCTGGTCC TATTGGACCT CCCGGTTTAC CTGGTCTGCG TGGAGATTCT 
GGCGGCAAAG GAGAAAAGGG ACACCCGGGT CTTATCGGTC TCATTGGACC CCCAGGAGAA 
CAGGGTGAGA AGGGAGACAG AGGCATGCCT GGGCCCCATG GATCCAATGG ACCAAAAGGA 
GAGACTGGAA TGCCTGGAGG CACAGGACCT ATCGGCCCCT CTGGTCCTCC TGGTCTGCCT 
GGTCCTCAAG GTATTAAAGG AGCCAAAGGT TCTTCTGGTG GAGCTGGTCC AAAAGGAGAA 
AAAGGAGTAC AAGGACCCCC TGGACTTCCT GGCCCACCAG GGGAGGTTAT TCAGCCACTA 
CCCATCCAAA GGAGTCCCAA GTCCAAGCGG TCCATTGATG CCAGCCAGCT ACTTCCTGAG 
AATGACATGC CTGCATCCGA TGCCACCGGG ACAGAATTCC TGATGGGTAG TGAAGGCATG 
GAGGAGATCT TTGGCTCCCT CAACTCGCTT CGGCAGGAGA TTGAGACCAT GCGCTTCCCT 
CTGGGCACCC AGGACAGCCC CGGCCGCACC TGCCAAGACC TGCACCTCAG TCAGCCAGAA 
CTCAAAGATG GAGAATACTG GATTGACCCC AACCAGGGCT GCTCCAGAGA CGCCTTCAAG 
GTCTTCTGTA ACTTTACTAA GGGGGCAGAG ACGTGCCTCT ACCCCAGCCA GAGCATCAGC 
ACGGTGAAGA TGAATTCCTG GAACAAAGAG ACTCCAGGTT CCTGGTACAG TCAGTTCTCC 
ACTGGCAGTA AGTTTTCATA CGTGGACTCC AACGGCGAAC CTGTGGGTGT GGTTCAGTTG 
GGTTTCCTTC GTCTCTTGAG CGTGCAGGCC CACCAGACTC TCACATACCA CTGCCACCGC 
TCGGTAGCGT GGGCTGACCG CAACGCCAAG AACACATACA GCCGAGCTCT GCACTTCAGG 
GGTGCCAACG ACGAGGATCT AAGCTATGAG ACCAATCCAT ACGTCAAGGC TTTGGTTGAT 
GGCTGCTCTT ATCGTAAGGG CTTTGACCGG ACAGTCCTGG AGATCAACAC ACCACAGGTG 
GAACATCTTC CTCTGCTGGA TATCAAGGTG TCAGACTTTG GGGAGAGCAA CCAGCAGTTT 
GGCTTTGAAG TGGGACCTGT CTGTTTCCAA GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615673.2
CDS	408..5603
Translation	MDTGDCPCRKKRRPWRMDFSSFALVTVMLIVCQTALVRADPVDVLRALQVPSLPEGVKKVPGFCTSRRSSSPDHAYRITKKAQISAPTKQLFSGRFPENFSIMALVKAQAGLQAFLLSIYSEQGIQQLGIEMGRSPVFLYEDQNGKPAPEDYPLFRGVNLADGKWHRIALSVSKKNVTLLLDCKKKMTRPLPRSNSAEVDTNGITVFGARLLDEEVFQGDIQQLLIASNPQAAYDFCEHYSPDCDSPLPKTQAQDPNTYPQVEPVLEGEEVHSTKAVGEGEEAFTEEYVTGDVTLKEYDYSYRDYNEPLVETGEVDANMGPALSAVTDEGGAGVRGQKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPSGPPGSVGDPGERGPPGKAGLPGADGVPGPPGTSVMLPFRFGQSGGDKGPAVSAQEAQAAAILSQARMALKGPPGPMGFTGRPGPLGNPGSPGLKGESGDPGPQGPRGSLGLMGPPGKSGRRGRAGADGARGMPGESGSKGDRGFDGLPGLPGDKGHRGDSGPMGPPGSQGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGIPGPPGVRGSDGPHGPKGNLGPQGEPGPPGQQGTPGTQGMPGPQGHTGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGPIGYPGPRGLKGGQGIRGLKGHKGEKGEDGFPGIKGDFGIKGERGEIGVPGPRGEDGPEGPKGRFGPPGETGPLGAVGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGSNGEKGTRGLTGKSGPRGQRGPTGPRGQRGPRGATGKLGPKGTSGSDGPPGPPGERGLPGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKMGPPGPPGVVGPQGPAGETGPNGERGHPGPPGPPGEQGLPGPSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGGGGFKGSEGPAGPPGPAGSPGERGQSGTAGPVGPPGRPGPQGPPGPAGEKGVSGEKGPIGPAGRDGLQGPVGLPGPAGPPGVPGEDGDKGEVGEHGQKGAKGGKGEHGPPGPPGPMGPVGQPGAPGADGELGPRGQQGPFGAKGDEGTRGFPGAPGPIGLQGLPGPPGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGIGGEPGKKGPRGERGEKGEAGQPGTAGSTGGRGLPGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGERGEDGEAGESGSPGPPGENGPPGPLGKRGPAGTRGPEGRQGEKGTKGDPGAVGPPGKTGPVGPQGVPGKPGTEGLRGLPGSVGEQGSPGPAGQKGPPGPIGPPGLPGLRGDSGGKGEKGHPGLIGLIGPPGEQGEKGDRGMPGPHGSNGPKGETGMPGGTGPIGPSGPPGLPGPQGIKGAKGSSGGAGPKGEKGVQGPPGLPGPPGEVIQPLPIQRSPKSKRSIDASQLLPENDMPASDATGTEFLMGSEGMEEIFGSLNSLRQEIETMRFPLGTQDSPGRTCQDLHLSQPELKDGEYWIDPNQGCSRDAFKVFCNFTKGAETCLYPSQSISTVKMNSWNKETPGSWYSQFSTGSKFSYVDSNGEPVGVVQLGFLRLLSVQAHQTLTYHCHRSVAWADRNAKNTYSRALHFRGANDEDLSYETNPYVKALVDGCSYRKGFDRTVLEINTPQVEHLPLLDIKVSDFGESNQQFGFEVGPVCFQG

Target ORF information:

RefSeq Version	XM_011617371.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617371.2

ORF Insert Sequence:

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGAC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATCCC 
CAGGTGGAGC CTGTGTTGGA GGGAGAAGAG GTGCATTCGA CCAAGGCTGT CGGTGAGGGA 
GAGGAGGCCT TCACAGAGGA GTATGTGACT GGAGATGTGA CCCTTAAGGA ATACGACTAT 
TCCTACAGGG ACTACAATGA GCCATTAGTG GAGACTGGAG AAGTCGATGC CAACATGGGC 
CCCGCCCTCT CTGCTGTGAC AGACGAGGGA GGCGCTGGGG TGAGAGGCCA GAAAGGAGAG 
AAAGGAGAAC CTGCTGTTTT GGAGCCTGGG ATGCTGATTG AAGGACCACC AGGACCTGAG 
GGACCTGCTG GCCTACCTGG TCCTTCTGGC CCTTCTGGAC CTCCTGGCTC TGTGGGTGAC 
CCAGGAGAGA GGGGCCCTCC TGGTAAGGCT GGTCTTCCTG GAGCTGATGG AGTCCCTGGA 
CCTCCTGGGA CCTCTGTTAT GCTGCCTTTC CGTTTTGGTC AGAGTGGAGG AGATAAAGGT 
CCTGCTGTTT CTGCTCAGGA AGCTCAGGCA GCAGCCATCT TGTCTCAGGC CAGGATGGCT 
CTTAAAGGAC CTCCTGGTCC AATGGGATTC ACTGGGCGCC CCGGACCCTT GGGAAATCCT 
GGAAGTCCTG GTCTGAAAGG AGAGAGTGGA GACCCTGGAC CCCAGGGCCC GAGGGGATCT 
CTAGGCTTGA TGGGACCTCC AGGCAAATCA GGAAGAAGAG GCCGCGCTGG AGCCGACGGT 
GCCAGAGGAA TGCCTGGAGA GTCTGGTTCT AAGGGTGATC GTGGTTTTGA TGGTCTTCCT 
GGTTTACCTG GAGACAAAGG ACACAGGGGT GACTCGGGAC CAATGGGACC TCCAGGATCT 
CAGGGAGAGG ATGGAGAAAG GGGAGACGAC GGAGACGTTG GACCCCGGGG TCTTCCAGGT 
GAACCAGGAC CTCGTGGTTT GCTTGGACCT AAAGGTCCTC CTGGAATCCC AGGCCCTCCT 
GGTGTACGAG GAAGTGACGG ACCCCACGGT CCAAAAGGAA ACTTGGGTCC TCAAGGTGAG 
CCAGGACCCC CTGGTCAGCA GGGAACCCCA GGAACTCAGG GAATGCCAGG ACCTCAAGGA 
CATACTGGAC CTCCAGGAGA AAAGGGTCCA ACAGGAAAAC CGGGTTTACC AGGAATGCCT 
GGAGCTGATG GCCCTCCTGG TCACCCAGGA AAGGAAGGCC CATCAGGCAC CAAAGGAAAC 
CAGGGTCCCA GTGGTCCCCA GGGGCCTATT GGCTATCCTG GTCCTCGTGG CCTCAAGGGC 
GGTCAAGGAA TCCGGGGATT GAAGGGTCAC AAAGGAGAGA AGGGAGAAGA TGGTTTCCCT 
GGAATTAAGG GAGACTTTGG GATCAAGGGT GAGAGGGGTG AGATTGGAGT ACCAGGTCCT 
AGAGGAGAGG ACGGTCCAGA GGGGCCAAAA GGTCGTTTTG GTCCCCCTGG TGAAACTGGA 
CCACTCGGAG CAGTAGGAGA GAAGGGTAAA CTTGGTGTAC CGGGACTTCC TGGATATCCT 
GGAAGACAAG GACCAAAGGG ATCTCTTGGA TTCCCAGGAT TCCCTGGATC CAATGGCGAG 
AAGGGAACAA GGGGTCTCAC TGGCAAATCA GGACCAAGAG GACAAAGAGG ACCAACGGGC 
CCCAGAGGGC AGAGGGGACC GAGGGGCGCA ACTGGCAAAC TGGGGCCAAA GGGGACTTCG 
GGAAGTGATG GCCCCCCTGG TCCTCCTGGA GAAAGGGGCT TGCCTGGGCC TCAGGGAGCC 
AATGGGTTCC CCGGACCTAA AGGGCCACCG GGGCCACCAG GGAAGGACGG GCTGCCTGGA 
CACCCTGGGC AGAGAGGAGA AGTGGGTTTC CAAGGTAAAA TGGGTCCACC TGGGCCTCCT 
GGAGTTGTTG GACCTCAGGG TCCAGCTGGA GAGACTGGCC CTAATGGTGA ACGTGGCCAC 
CCTGGACCAC CAGGTCCACC AGGAGAACAG GGACTTCCTG GTCCCTCAGG AAAGGAGGGA 
ACCAAAGGAG ACCCTGGACC CCCAGGAGGC CCTGGCAAAG ATGGGCCTCC TGGACTGAGG 
GGCTTCCCTG GTGAGAGAGG ACTGCCAGGA ACACCTGGAG GTGGAGGATT CAAAGGAAGC 
GAAGGACCTG CTGGACCTCC TGGACCGGCT GGCTCTCCTG GTGAGAGGGG GCAATCTGGT 
ACCGCTGGGC CTGTTGGACC TCCTGGAAGA CCAGGTCCTC AGGGACCTCC TGGACCTGCA 
GGAGAGAAAG GTGTTTCTGG TGAGAAAGGC CCAATTGGCC CAGCTGGTCG TGACGGACTA 
CAGGGTCCTG TGGGTCTGCC TGGCCCAGCT GGACCGCCCG GAGTACCTGG AGAAGATGGA 
GATAAGGGGG AGGTTGGAGA ACACGGTCAG AAAGGTGCCA AGGGAGGGAA AGGAGAGCAT 
GGTCCTCCAG GTCCACCTGG ACCAATGGGA CCAGTCGGCC AGCCTGGTGC TCCAGGTGCT 
GATGGAGAGC TTGGGCCGAG GGGTCAGCAG GGACCTTTTG GAGCTAAAGG GGATGAAGGA 
ACCAGAGGAT TCCCAGGAGC CCCAGGACCC ATTGGACTCC AGGGTTTGCC TGGGCCACCA 
GGTGAGAAGG GAGAAACCGG AGATGTTGGA CCTCTGGGAC CTCCAGGCCC TCCAGGACCC 
CGTGGCCCTG CTGGACCCAA CGGTGCTGAT GGCCCTCAAG GGCCTCCTGG TGGTTTGGGT 
AATCCTGGAC CTATCGGTGA GAAGGGAGAG CCTGGAGAGT CTGGACCCCC TGGAATTGGA 
GGGGAGCCTG GAAAGAAGGG CCCCAGAGGG GAACGTGGGG AGAAGGGGGA GGCTGGACAA 
CCAGGAACCG CTGGATCCAC TGGAGGACGT GGACTACCTG GAGATGATGG ACCCAAAGGA 
AACCCAGGTC CTGTTGGTTT CCCCGGTGAT CCTGGTCCAC CGGGTGAGGT TGGACCCAGA 
GGTCAAGACG GTGCCAAGGG AGAAAGAGGA GAAGATGGTG AAGCAGGAGA GTCTGGATCC 
CCTGGACCTC CCGGTGAGAA CGGACCCCCC GGCCCCCTAG GAAAGAGGGG TCCTGCTGGA 
ACAAGAGGAC CAGAGGGACG CCAGGGAGAG AAGGGAACAA AGGGGGATCC AGGTGCTGTT 
GGCCCCCCAG GCAAAACAGG CCCTGTGGGT CCACAGGGAG TGCCAGGAAA ACCTGGAACA 
GAAGGCCTTA GAGGGCTTCC AGGATCAGTG GGTGAGCAAG GATCTCCAGG ACCTGCAGGA 
CAGAAAGGAC CACCTGGTCC TATTGGACCT CCCGGTTTAC CTGGTCTGCG TGGAGATTCT 
GGCGGCAAAG GAGAAAAGGG ACACCCGGGT CTTATCGGTC TCATTGGACC CCCAGGAGAA 
CAGGGTGAGA AGGGAGACAG AGGCATGCCT GGGCCCCATG GATCCAATGG ACCAAAAGGA 
GAGACTGGAA TGCCTGGAGG CACAGGACCT ATCGGCCCCT CTGGTCCTCC TGGTCTGCCT 
GGTCCTCAAG GTATTAAAGG AGCCAAAGGT TCTTCTGGTG GAGCTGGTCC AAAAGGAGAA 
AAAGGAGTAC AAGGACCCCC TGGACTTCCT GGCCCACCAG GGGAGGTTAT TCAGCCACTA 
CCCATCCAAA GGAGTCCCAA GTCCAAGCGG TCCATTGATG CCAGCCAGCT ACTTCCTGAG 
AATGACATGC CTGCATCCGA TGCCACCGGG ACAGAATTCC TGATGGGTAG TGAAGGCATG 
GAGGAGATCT TTGGCTCCCT CAACTCGCTT CGGCAGGAGA TTGAGACCAT GCGCTTCCCT 
CTGGGCACCC AGGACAGCCC CGGCCGCACC TGCCAAGACC TGCACCTCAG TCAGCCAGAA 
CTCAAAGATG GAGAATACTG GATTGACCCC AACCAGGGCT GCTCCAGAGA CGCCTTCAAG 
GTCTTCTGTA ACTTTACTAA GGGGGCAGAG ACGTGCCTCT ACCCCAGCCA GAGCATCAGC 
ACGGTGAAGA TGAATTCCTG GAACAAAGAG ACTCCAGGTT CCTGGTACAG TCAGTTCTCC 
ACTGGCAGTA AGTTTTCATA CGTGGACTCC AACGGCGAAC CTGTGGGTGT GGTTCAGTTG 
GGTTTCCTTC GTCTCTTGAG CGTGCAGGCC CACCAGACTC TCACATACCA CTGCCACCGC 
TCGGTAGCGT GGGCTGACCG CAACGCCAAG AACACATACA GCCGAGCTCT GCACTTCAGG 
GGTGCCAACG ACGAGGATCT AAGCTATGAG ACCAATCCAT ACGTCAAGGC TTTGGTTGAT 
GGCTGCTCTT ATCGTAAGGG CTTTGACCGG ACAGTCCTGG AGATCAACAC ACCACAGGTG 
GAACATCTTC CTCTGCTGGA TATCAAGGTG TCAGACTTTG GGGAGAGCAA CCAGCAGTTT 
GGCTTTGAAG TGGGACCTGT CTGTTTCCAA GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101066527 ( XM_029844529.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029700389.1 Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X7, mRNA.

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CloneID	OTo45743
Clone ID Related Accession (Same CDS sequence)	XM_029844529.1
Accession Version	XM_029844529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	collagen alpha-1(XI) chain isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042296.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGAC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATGCT 
GGGGTGAGAG GCCAGAAAGG AGAGAAAGGA GAACCTGCTG TTTTGGAGCC TGGGATGCTG 
ATTGAAGGAC CACCAGGACC TGAGGGACCT GCTGGCCTAC CTGGTCCTTC TGGCCCTTCT 
GGACCTCCTG GCTCTGTGGG TGACCCAGGA GAGAGGGGCC CTCCTGGTAA GGCTGGTCTT 
CCTGGAGCTG ATGGAGTCCC TGGACCTCCT GGGACCTCTG TTATGCTGCC TTTCCGTTTT 
GGTCAGAGTG GAGGAGATAA AGGTCCTGCT GTTTCTGCTC AGGAAGCTCA GGCAGCAGCC 
ATCTTGTCTC AGGCCAGGAT GGCTCTTAAA GGACCTCCTG GTCCAATGGG ATTCACTGGG 
CGCCCCGGAC CCTTGGGAAA TCCTGGAAGT CCTGGTCTGA AAGGAGAGAG TGGAGACCCT 
GGACCCCAGG GCCCGAGGGG ATCTCTAGGC TTGATGGGAC CTCCAGGCAA ATCAGGAAGA 
AGAGGCCGCG CTGGAGCCGA CGGTGCCAGA GGAATGCCTG GAGAGTCTGG TTCTAAGGGT 
GATCGTGGTT TTGATGGTCT TCCTGGTTTA CCTGGAGACA AAGGACACAG GGGTGACTCG 
GGACCAATGG GACCTCCAGG ATCTCAGGGA GAGGATGGAG AAAGGGGAGA CGACGGAGAC 
GTTGGACCCC GGGGTCTTCC AGGTGAACCA GGACCTCGTG GTTTGCTTGG ACCTAAAGGT 
CCTCCTGGAA TCCCAGGCCC TCCTGGTGTA CGAGGAAGTG ACGGACCCCA CGGTCCAAAA 
GGAAACTTGG GTCCTCAAGG TGAGCCAGGA CCCCCTGGTC AGCAGGGAAC CCCAGGAACT 
CAGGGAATGC CAGGACCTCA AGGACATACT GGACCTCCAG GAGAAAAGGG TCCAACAGGA 
AAACCGGGTT TACCAGGAAT GCCTGGAGCT GATGGCCCTC CTGGTCACCC AGGAAAGGAA 
GGCCCATCAG GCACCAAAGG AAACCAGGGT CCCAGTGGTC CCCAGGGGCC TATTGGCTAT 
CCTGGTCCTC GTGGCCTCAA GGGCGGTCAA GGAATCCGGG GATTGAAGGG TCACAAAGGA 
GAGAAGGGAG AAGATGGTTT CCCTGGAATT AAGGGAGACT TTGGGATCAA GGGTGAGAGG 
GGTGAGATTG GAGTACCAGG TCCTAGAGGA GAGGACGGTC CAGAGGGGCC AAAAGGTCGT 
TTTGGTCCCC CTGGTGAAAC TGGACCACTC GGAGCAGTAG GAGAGAAGGG TAAACTTGGT 
GTACCGGGAC TTCCTGGATA TCCTGGAAGA CAAGGACCAA AGGGATCTCT TGGATTCCCA 
GGATTCCCTG GATCCAATGG CGAGAAGGGA ACAAGGGGTC TCACTGGCAA ATCAGGACCA 
AGAGGACAAA GAGGACCAAC GGGCCCCAGA GGGCAGAGGG GACCGAGGGG CGCAACTGGC 
AAACTGGGGC CAAAGGGGAC TTCGGGAAGT GATGGCCCCC CTGGTCCTCC TGGAGAAAGG 
GGCTTGCCTG GGCCTCAGGG AGCCAATGGG TTCCCCGGAC CTAAAGGGCC ACCGGGGCCA 
CCAGGGAAGG ACGGGCTGCC TGGACACCCT GGGCAGAGAG GAGAAGTGGG TTTCCAAGGT 
AAAATGGGTC CACCTGGGCC TCCTGGAGTT GTTGGACCTC AGGGTCCAGC TGGAGAGACT 
GGCCCTAATG GTGAACGTGG CCACCCTGGA CCACCAGGTC CACCAGGAGA ACAGGGACTT 
CCTGGTCCCT CAGGAAAGGA GGGAACCAAA GGAGACCCTG GACCCCCAGG AGGCCCTGGC 
AAAGATGGGC CTCCTGGACT GAGGGGCTTC CCTGGTGAGA GAGGACTGCC AGGAACACCT 
GGAGGTGGAG GATTCAAAGG AAGCGAAGGA CCTGCTGGAC CTCCTGGACC GGCTGGCTCT 
CCTGGTGAGA GGGGGCAATC TGGTACCGCT GGGCCTGTTG GACCTCCTGG AAGACCAGGT 
CCTCAGGGAC CTCCTGGACC TGCAGGAGAG AAAGGTGTTT CTGGTGAGAA AGGCCCAATT 
GGCCCAGCTG GTCGTGACGG ACTACAGGGT CCTGTGGGTC TGCCTGGCCC AGCTGGACCG 
CCCGGAGTAC CTGGAGAAGA TGGAGATAAG GGGGAGGTTG GAGAACACGG TCAGAAAGGT 
GCCAAGGGAG GGAAAGGAGA GCATGGTCCT CCAGGTCCAC CTGGACCAAT GGGACCAGTC 
GGCCAGCCTG GTGCTCCAGG TGCTGATGGA GAGCTTGGGC CGAGGGGTCA GCAGGGACCT 
TTTGGAGCTA AAGGGGATGA AGGAACCAGA GGATTCCCAG GAGCCCCAGG ACCCATTGGA 
CTCCAGGGTT TGCCTGGGCC ACCAGGTGAG AAGGGAGAAA CCGGAGATGT TGGACCTCTG 
GGACCTCCAG GCCCTCCAGG ACCCCGTGGC CCTGCTGGAC CCAACGGTGC TGATGGCCCT 
CAAGGGCCTC CTGGTGGTTT GGGTAATCCT GGACCTATCG GTGAGAAGGG AGAGCCTGGA 
GAGTCTGGAC CCCCTGGAAT TGGAGGGGAG CCTGGAAAGA AGGGCCCCAG AGGGGAACGT 
GGGGAGAAGG GGGAGGCTGG ACAACCAGGA ACCGCTGGAT CCACTGGAGG ACGTGGACTA 
CCTGGAGATG ATGGACCCAA AGGAAACCCA GGTCCTGTTG GTTTCCCCGG TGATCCTGGT 
CCACCGGGTG AGGTTGGACC CAGAGGTCAA GACGGTGCCA AGGGAGAAAG AGGAGAAGAT 
GGTGAAGCAG GAGAGTCTGG ATCCCCTGGA CCTCCCGGTG AGAACGGACC CCCCGGCCCC 
CTAGGAAAGA GGGGTCCTGC TGGAACAAGA GGACCAGAGG GACGCCAGGG AGAGAAGGGA 
ACAAAGGGGG ATCCAGGTGC TGTTGGCCCC CCAGGCAAAA CAGGCCCTGT GGGTCCACAG 
GGAGTGCCAG GAAAACCTGG AACAGAAGGC CTTAGAGGGC TTCCAGGATC AGTGGGTGAG 
CAAGGATCTC CAGGACCTGC AGGACAGAAA GGACCACCTG GTCCTATTGG ACCTCCCGGT 
TTACCTGGTC TGCGTGGAGA TTCTGGCGGC AAAGGAGAAA AGGGACACCC GGGTCTTATC 
GGTCTCATTG GACCCCCAGG AGAACAGGGT GAGAAGGGAG ACAGAGGCAT GCCTGGGCCC 
CATGGATCCA ATGGACCAAA AGGAGAGACT GGAATGCCTG GAGGCACAGG ACCTATCGGC 
CCCTCTGGTC CTCCTGGTCT GCCTGGTCCT CAAGGTATTA AAGGAGCCAA AGGTTCTTCT 
GGTGGAGCTG GTCCAAAAGG AGAAAAAGGA GTACAAGGAC CCCCTGGACT TCCTGGCCCA 
CCAGGGGAGG TTATTCAGCC ACTACCCATC CAAAGGAGTC CCAAGTCCAA GCGGTCCATT 
GATGCCAGCC AGCTACTTCC TGAGAATGAC ATGCCTGCAT CCGATGCCAC CGGGACAGAA 
TTCCTGATGG GTAGTGAAGG CATGGAGGAG ATCTTTGGCT CCCTCAACTC GCTTCGGCAG 
GAGATTGAGA CCATGCGCTT CCCTCTGGGC ACCCAGGACA GCCCCGGCCG CACCTGCCAA 
GACCTGCACC TCAGTCAGCC AGAACTCAAA GATGGAGAAT ACTGGATTGA CCCCAACCAG 
GGCTGCTCCA GAGACGCCTT CAAGGTCTTC TGTAACTTTA CTAAGGGGGC AGAGACGTGC 
CTCTACCCCA GCCAGAGCAT CAGCACGGTG AAGATGAATT CCTGGAACAA AGAGACTCCA 
GGTTCCTGGT ACAGTCAGTT CTCCACTGGC AGTAAGTTTT CATACGTGGA CTCCAACGGC 
GAACCTGTGG GTGTGGTTCA GTTGGGTTTC CTTCGTCTCT TGAGCGTGCA GGCCCACCAG 
ACTCTCACAT ACCACTGCCA CCGCTCGGTA GCGTGGGCTG ACCGCAACGC CAAGAACACA 
TACAGCCGAG CTCTGCACTT CAGGGGTGCC AACGACGAGG ATCTAAGCTA TGAGACCAAT 
CCATACGTCA AGGCTTTGGT TGATGGCTGC TCTTATCGTA AGGGCTTTGA CCGGACAGTC 
CTGGAGATCA ACACACCACA GGTGGAACAT CTTCCTCTGC TGGATATCAA GGTGTCAGAC 
TTTGGGGAGA GCAACCAGCA GTTTGGCTTT GAAGTGGGAC CTGTCTGTTT CCAAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029700389.1
CDS	408..5387
Translation	MDTGDCPCRKKRRPWRMDFSSFALVTVMLIVCQTALVRADPVDVLRALQVPSLPEGVKKVPGFCTSRRSSSPDHAYRITKKAQISAPTKQLFSGRFPENFSIMALVKAQAGLQAFLLSIYSEQGIQQLGIEMGRSPVFLYEDQNGKPAPEDYPLFRGVNLADGKWHRIALSVSKKNVTLLLDCKKKMTRPLPRSNSAEVDTNGITVFGARLLDEEVFQGDIQQLLIASNPQAAYDFCEHYSPDCDSPLPKTQAQDPNTYAGVRGQKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPSGPPGSVGDPGERGPPGKAGLPGADGVPGPPGTSVMLPFRFGQSGGDKGPAVSAQEAQAAAILSQARMALKGPPGPMGFTGRPGPLGNPGSPGLKGESGDPGPQGPRGSLGLMGPPGKSGRRGRAGADGARGMPGESGSKGDRGFDGLPGLPGDKGHRGDSGPMGPPGSQGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGIPGPPGVRGSDGPHGPKGNLGPQGEPGPPGQQGTPGTQGMPGPQGHTGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGPIGYPGPRGLKGGQGIRGLKGHKGEKGEDGFPGIKGDFGIKGERGEIGVPGPRGEDGPEGPKGRFGPPGETGPLGAVGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGSNGEKGTRGLTGKSGPRGQRGPTGPRGQRGPRGATGKLGPKGTSGSDGPPGPPGERGLPGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKMGPPGPPGVVGPQGPAGETGPNGERGHPGPPGPPGEQGLPGPSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGGGGFKGSEGPAGPPGPAGSPGERGQSGTAGPVGPPGRPGPQGPPGPAGEKGVSGEKGPIGPAGRDGLQGPVGLPGPAGPPGVPGEDGDKGEVGEHGQKGAKGGKGEHGPPGPPGPMGPVGQPGAPGADGELGPRGQQGPFGAKGDEGTRGFPGAPGPIGLQGLPGPPGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGIGGEPGKKGPRGERGEKGEAGQPGTAGSTGGRGLPGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGERGEDGEAGESGSPGPPGENGPPGPLGKRGPAGTRGPEGRQGEKGTKGDPGAVGPPGKTGPVGPQGVPGKPGTEGLRGLPGSVGEQGSPGPAGQKGPPGPIGPPGLPGLRGDSGGKGEKGHPGLIGLIGPPGEQGEKGDRGMPGPHGSNGPKGETGMPGGTGPIGPSGPPGLPGPQGIKGAKGSSGGAGPKGEKGVQGPPGLPGPPGEVIQPLPIQRSPKSKRSIDASQLLPENDMPASDATGTEFLMGSEGMEEIFGSLNSLRQEIETMRFPLGTQDSPGRTCQDLHLSQPELKDGEYWIDPNQGCSRDAFKVFCNFTKGAETCLYPSQSISTVKMNSWNKETPGSWYSQFSTGSKFSYVDSNGEPVGVVQLGFLRLLSVQAHQTLTYHCHRSVAWADRNAKNTYSRALHFRGANDEDLSYETNPYVKALVDGCSYRKGFDRTVLEINTPQVEHLPLLDIKVSDFGESNQQFGFEVGPVCFQG

Target ORF information:

RefSeq Version	XM_029844529.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029844529.1

ORF Insert Sequence:

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGAC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATGCT 
GGGGTGAGAG GCCAGAAAGG AGAGAAAGGA GAACCTGCTG TTTTGGAGCC TGGGATGCTG 
ATTGAAGGAC CACCAGGACC TGAGGGACCT GCTGGCCTAC CTGGTCCTTC TGGCCCTTCT 
GGACCTCCTG GCTCTGTGGG TGACCCAGGA GAGAGGGGCC CTCCTGGTAA GGCTGGTCTT 
CCTGGAGCTG ATGGAGTCCC TGGACCTCCT GGGACCTCTG TTATGCTGCC TTTCCGTTTT 
GGTCAGAGTG GAGGAGATAA AGGTCCTGCT GTTTCTGCTC AGGAAGCTCA GGCAGCAGCC 
ATCTTGTCTC AGGCCAGGAT GGCTCTTAAA GGACCTCCTG GTCCAATGGG ATTCACTGGG 
CGCCCCGGAC CCTTGGGAAA TCCTGGAAGT CCTGGTCTGA AAGGAGAGAG TGGAGACCCT 
GGACCCCAGG GCCCGAGGGG ATCTCTAGGC TTGATGGGAC CTCCAGGCAA ATCAGGAAGA 
AGAGGCCGCG CTGGAGCCGA CGGTGCCAGA GGAATGCCTG GAGAGTCTGG TTCTAAGGGT 
GATCGTGGTT TTGATGGTCT TCCTGGTTTA CCTGGAGACA AAGGACACAG GGGTGACTCG 
GGACCAATGG GACCTCCAGG ATCTCAGGGA GAGGATGGAG AAAGGGGAGA CGACGGAGAC 
GTTGGACCCC GGGGTCTTCC AGGTGAACCA GGACCTCGTG GTTTGCTTGG ACCTAAAGGT 
CCTCCTGGAA TCCCAGGCCC TCCTGGTGTA CGAGGAAGTG ACGGACCCCA CGGTCCAAAA 
GGAAACTTGG GTCCTCAAGG TGAGCCAGGA CCCCCTGGTC AGCAGGGAAC CCCAGGAACT 
CAGGGAATGC CAGGACCTCA AGGACATACT GGACCTCCAG GAGAAAAGGG TCCAACAGGA 
AAACCGGGTT TACCAGGAAT GCCTGGAGCT GATGGCCCTC CTGGTCACCC AGGAAAGGAA 
GGCCCATCAG GCACCAAAGG AAACCAGGGT CCCAGTGGTC CCCAGGGGCC TATTGGCTAT 
CCTGGTCCTC GTGGCCTCAA GGGCGGTCAA GGAATCCGGG GATTGAAGGG TCACAAAGGA 
GAGAAGGGAG AAGATGGTTT CCCTGGAATT AAGGGAGACT TTGGGATCAA GGGTGAGAGG 
GGTGAGATTG GAGTACCAGG TCCTAGAGGA GAGGACGGTC CAGAGGGGCC AAAAGGTCGT 
TTTGGTCCCC CTGGTGAAAC TGGACCACTC GGAGCAGTAG GAGAGAAGGG TAAACTTGGT 
GTACCGGGAC TTCCTGGATA TCCTGGAAGA CAAGGACCAA AGGGATCTCT TGGATTCCCA 
GGATTCCCTG GATCCAATGG CGAGAAGGGA ACAAGGGGTC TCACTGGCAA ATCAGGACCA 
AGAGGACAAA GAGGACCAAC GGGCCCCAGA GGGCAGAGGG GACCGAGGGG CGCAACTGGC 
AAACTGGGGC CAAAGGGGAC TTCGGGAAGT GATGGCCCCC CTGGTCCTCC TGGAGAAAGG 
GGCTTGCCTG GGCCTCAGGG AGCCAATGGG TTCCCCGGAC CTAAAGGGCC ACCGGGGCCA 
CCAGGGAAGG ACGGGCTGCC TGGACACCCT GGGCAGAGAG GAGAAGTGGG TTTCCAAGGT 
AAAATGGGTC CACCTGGGCC TCCTGGAGTT GTTGGACCTC AGGGTCCAGC TGGAGAGACT 
GGCCCTAATG GTGAACGTGG CCACCCTGGA CCACCAGGTC CACCAGGAGA ACAGGGACTT 
CCTGGTCCCT CAGGAAAGGA GGGAACCAAA GGAGACCCTG GACCCCCAGG AGGCCCTGGC 
AAAGATGGGC CTCCTGGACT GAGGGGCTTC CCTGGTGAGA GAGGACTGCC AGGAACACCT 
GGAGGTGGAG GATTCAAAGG AAGCGAAGGA CCTGCTGGAC CTCCTGGACC GGCTGGCTCT 
CCTGGTGAGA GGGGGCAATC TGGTACCGCT GGGCCTGTTG GACCTCCTGG AAGACCAGGT 
CCTCAGGGAC CTCCTGGACC TGCAGGAGAG AAAGGTGTTT CTGGTGAGAA AGGCCCAATT 
GGCCCAGCTG GTCGTGACGG ACTACAGGGT CCTGTGGGTC TGCCTGGCCC AGCTGGACCG 
CCCGGAGTAC CTGGAGAAGA TGGAGATAAG GGGGAGGTTG GAGAACACGG TCAGAAAGGT 
GCCAAGGGAG GGAAAGGAGA GCATGGTCCT CCAGGTCCAC CTGGACCAAT GGGACCAGTC 
GGCCAGCCTG GTGCTCCAGG TGCTGATGGA GAGCTTGGGC CGAGGGGTCA GCAGGGACCT 
TTTGGAGCTA AAGGGGATGA AGGAACCAGA GGATTCCCAG GAGCCCCAGG ACCCATTGGA 
CTCCAGGGTT TGCCTGGGCC ACCAGGTGAG AAGGGAGAAA CCGGAGATGT TGGACCTCTG 
GGACCTCCAG GCCCTCCAGG ACCCCGTGGC CCTGCTGGAC CCAACGGTGC TGATGGCCCT 
CAAGGGCCTC CTGGTGGTTT GGGTAATCCT GGACCTATCG GTGAGAAGGG AGAGCCTGGA 
GAGTCTGGAC CCCCTGGAAT TGGAGGGGAG CCTGGAAAGA AGGGCCCCAG AGGGGAACGT 
GGGGAGAAGG GGGAGGCTGG ACAACCAGGA ACCGCTGGAT CCACTGGAGG ACGTGGACTA 
CCTGGAGATG ATGGACCCAA AGGAAACCCA GGTCCTGTTG GTTTCCCCGG TGATCCTGGT 
CCACCGGGTG AGGTTGGACC CAGAGGTCAA GACGGTGCCA AGGGAGAAAG AGGAGAAGAT 
GGTGAAGCAG GAGAGTCTGG ATCCCCTGGA CCTCCCGGTG AGAACGGACC CCCCGGCCCC 
CTAGGAAAGA GGGGTCCTGC TGGAACAAGA GGACCAGAGG GACGCCAGGG AGAGAAGGGA 
ACAAAGGGGG ATCCAGGTGC TGTTGGCCCC CCAGGCAAAA CAGGCCCTGT GGGTCCACAG 
GGAGTGCCAG GAAAACCTGG AACAGAAGGC CTTAGAGGGC TTCCAGGATC AGTGGGTGAG 
CAAGGATCTC CAGGACCTGC AGGACAGAAA GGACCACCTG GTCCTATTGG ACCTCCCGGT 
TTACCTGGTC TGCGTGGAGA TTCTGGCGGC AAAGGAGAAA AGGGACACCC GGGTCTTATC 
GGTCTCATTG GACCCCCAGG AGAACAGGGT GAGAAGGGAG ACAGAGGCAT GCCTGGGCCC 
CATGGATCCA ATGGACCAAA AGGAGAGACT GGAATGCCTG GAGGCACAGG ACCTATCGGC 
CCCTCTGGTC CTCCTGGTCT GCCTGGTCCT CAAGGTATTA AAGGAGCCAA AGGTTCTTCT 
GGTGGAGCTG GTCCAAAAGG AGAAAAAGGA GTACAAGGAC CCCCTGGACT TCCTGGCCCA 
CCAGGGGAGG TTATTCAGCC ACTACCCATC CAAAGGAGTC CCAAGTCCAA GCGGTCCATT 
GATGCCAGCC AGCTACTTCC TGAGAATGAC ATGCCTGCAT CCGATGCCAC CGGGACAGAA 
TTCCTGATGG GTAGTGAAGG CATGGAGGAG ATCTTTGGCT CCCTCAACTC GCTTCGGCAG 
GAGATTGAGA CCATGCGCTT CCCTCTGGGC ACCCAGGACA GCCCCGGCCG CACCTGCCAA 
GACCTGCACC TCAGTCAGCC AGAACTCAAA GATGGAGAAT ACTGGATTGA CCCCAACCAG 
GGCTGCTCCA GAGACGCCTT CAAGGTCTTC TGTAACTTTA CTAAGGGGGC AGAGACGTGC 
CTCTACCCCA GCCAGAGCAT CAGCACGGTG AAGATGAATT CCTGGAACAA AGAGACTCCA 
GGTTCCTGGT ACAGTCAGTT CTCCACTGGC AGTAAGTTTT CATACGTGGA CTCCAACGGC 
GAACCTGTGG GTGTGGTTCA GTTGGGTTTC CTTCGTCTCT TGAGCGTGCA GGCCCACCAG 
ACTCTCACAT ACCACTGCCA CCGCTCGGTA GCGTGGGCTG ACCGCAACGC CAAGAACACA 
TACAGCCGAG CTCTGCACTT CAGGGGTGCC AACGACGAGG ATCTAAGCTA TGAGACCAAT 
CCATACGTCA AGGCTTTGGT TGATGGCTGC TCTTATCGTA AGGGCTTTGA CCGGACAGTC 
CTGGAGATCA ACACACCACA GGTGGAACAT CTTCCTCTGC TGGATATCAA GGTGTCAGAC 
TTTGGGGAGA GCAACCAGCA GTTTGGCTTT GAAGTGGGAC CTGTCTGTTT CCAAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101066527 ( XM_011617365.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011615667.2 Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X1, mRNA.

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CloneID	OTo45737
Clone ID Related Accession (Same CDS sequence)	XM_011617365.2
Accession Version	XM_011617365.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	collagen alpha-1(XI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042296.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011617365.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGAC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATAAG 
AAGCAGCTCA ATGGAAAAGG AGCCCCCACT AAATCCACTG CTGTTAAAGC AAAACCTACC 
CCAGCAAAGC CAGCCAAGTC TGGATCATAT AAGCTTGTAG TCAAGTCCCC TATGCCCATC 
AAAGCAGCAA AGTCTACAGC CAAGCCATTT AAAGGCAGGC CCACTGCAGT AGAGATCCTT 
CTCTCTAAGA TAAACCCAAT AACACCATCT AAATCCACAA TTTCCTTGAT TAAAAATGGC 
AACGGTAAAT CAGCTGCAGC AAAGACAAAT GGATTGGCCA AAGCCAACAA CAATGGAAAT 
GGAAGAGCAA CAGGCGCTAA AGCAAATGGC AATGGCAAAG CTGATTCTAA GCCCATAGCT 
CAGGCCAGAG TGAGCGGAAA TGGCAAAGTT GTAGTGGAGA AAAAAGTCAT CATAAATGCC 
AAATCTTCAG CAGAAAAGAA ATCCAGCGGA AATGGAAAAG TCACCATTGT AACCAAGAGT 
AATGGTGCAG CAGAGAAGAA AACCAGTGGT AGTGGCAAAG CTACCATTGA GGTTAAAGAA 
ATTAAAGTCA AAGTTCATGG AAAACCTGAA AATAAAGTTG CAGGTGAGGC CAAGGCTAAC 
GGCAATGGTA AAATTATCAC TGTGGTAGAA AAGAAATTCA CCAATGGTGC TGCGAATCGG 
GCAAAGATTG AAACCACTAC AAGAGCAAAG ACGGAGAAAG TTATCAAAGT TGAAAAGATA 
GAAAAGACTA CGCTCAGTAC AGCTAAATCA GCCACAAAAC TAGATGCTTC TAAAGCAGCC 
AAACCCACAA AAGCCACAAA ATCTGATGCT ACAACATTAA CAAAGGCCAC CAAAGCAGCG 
GCAGCAGCAG GAAAGACCCA AGCAAGGGCA GAGGTCAAAG AAGTCACCCA AGTTAAAACT 
GTTGTCACCA AAGTCCCTCT GGTCAAAACC ACTGTGAGCA AACCAGCGAA GAATGTGGTG 
GAGAGAGTTA CGATGCCCAG GAAAGTAGCC TCGACGTCCA TGCCTGTGAG GCCCACTCCA 
CCCAGACCTA CGAAACCCAA GATGGCGGTG GACATTAATG TCAAATCCCA GCACAAACCT 
TTCCCACCAT TTGGCAAGAC TGCGTTGAGT GGAACTACGA AGAAAGTTAC CAACGGTTAT 
CAGCAGGATG TAGACACTGA ATATTCCGAG GCTGGCCACA CCCCTACAGA GAGAGATTAT 
TACTATGAGG AGACGGTACC ACTGCCAGAG GGTGAGGTGG TTGGGCAAGA GGAACTCGCC 
GTACAGCCCC AGGTGGAGCC TGTGTTGGAG GGAGAAGAGG TGCATTCGAC CAAGGCTGTC 
GGTGAGGGAG AGGAGGCCTT CACAGAGGAG TATGTGACTG GAGATGTGAC CCTTAAGGAA 
TACGACTATT CCTACAGGGA CTACAATGAG CCATTAGTGG AGACTGGAGA AGTCGATGCC 
AACATGGGCC CCGCCCTCTC TGCTGTGACA GACGAGGGAG GCGCTGGGGT GAGAGGCCAG 
AAAGGAGAGA AAGGAGAACC TGCTGTTTTG GAGCCTGGGA TGCTGATTGA AGGACCACCA 
GGACCTGAGG GACCTGCTGG CCTACCTGGT CCTTCTGGCC CTTCTGGACC TCCTGGCTCT 
GTGGGTGACC CAGGAGAGAG GGGCCCTCCT GGTAAGGCTG GTCTTCCTGG AGCTGATGGA 
GTCCCTGGAC CTCCTGGGAC CTCTGTTATG CTGCCTTTCC GTTTTGGTCA GAGTGGAGGA 
GATAAAGGTC CTGCTGTTTC TGCTCAGGAA GCTCAGGCAG CAGCCATCTT GTCTCAGGCC 
AGGATGGCTC TTAAAGGACC TCCTGGTCCA ATGGGATTCA CTGGGCGCCC CGGACCCTTG 
GGAAATCCTG GAAGTCCTGG TCTGAAAGGA GAGAGTGGAG ACCCTGGACC CCAGGGCCCG 
AGGGGATCTC TAGGCTTGAT GGGACCTCCA GGCAAATCAG GAAGAAGAGG CCGCGCTGGA 
GCCGACGGTG CCAGAGGAAT GCCTGGAGAG TCTGGTTCTA AGGGTGATCG TGGTTTTGAT 
GGTCTTCCTG GTTTACCTGG AGACAAAGGA CACAGGGGTG ACTCGGGACC AATGGGACCT 
CCAGGATCTC AGGGAGAGGA TGGAGAAAGG GGAGACGACG GAGACGTTGG ACCCCGGGGT 
CTTCCAGGTG AACCAGGACC TCGTGGTTTG CTTGGACCTA AAGGTCCTCC TGGAATCCCA 
GGCCCTCCTG GTGTACGAGG AAGTGACGGA CCCCACGGTC CAAAAGGAAA CTTGGGTCCT 
CAAGGTGAGC CAGGACCCCC TGGTCAGCAG GGAACCCCAG GAACTCAGGG AATGCCAGGA 
CCTCAAGGAC ATACTGGACC TCCAGGAGAA AAGGGTCCAA CAGGAAAACC GGGTTTACCA 
GGAATGCCTG GAGCTGATGG CCCTCCTGGT CACCCAGGAA AGGAAGGCCC ATCAGGCACC 
AAAGGAAACC AGGGTCCCAG TGGTCCCCAG GGGCCTATTG GCTATCCTGG TCCTCGTGGC 
CTCAAGGGCG GTCAAGGAAT CCGGGGATTG AAGGGTCACA AAGGAGAGAA GGGAGAAGAT 
GGTTTCCCTG GAATTAAGGG AGACTTTGGG ATCAAGGGTG AGAGGGGTGA GATTGGAGTA 
CCAGGTCCTA GAGGAGAGGA CGGTCCAGAG GGGCCAAAAG GTCGTTTTGG TCCCCCTGGT 
GAAACTGGAC CACTCGGAGC AGTAGGAGAG AAGGGTAAAC TTGGTGTACC GGGACTTCCT 
GGATATCCTG GAAGACAAGG ACCAAAGGGA TCTCTTGGAT TCCCAGGATT CCCTGGATCC 
AATGGCGAGA AGGGAACAAG GGGTCTCACT GGCAAATCAG GACCAAGAGG ACAAAGAGGA 
CCAACGGGCC CCAGAGGGCA GAGGGGACCG AGGGGCGCAA CTGGCAAACT GGGGCCAAAG 
GGGACTTCGG GAAGTGATGG CCCCCCTGGT CCTCCTGGAG AAAGGGGCTT GCCTGGGCCT 
CAGGGAGCCA ATGGGTTCCC CGGACCTAAA GGGCCACCGG GGCCACCAGG GAAGGACGGG 
CTGCCTGGAC ACCCTGGGCA GAGAGGAGAA GTGGGTTTCC AAGGTAAAAT GGGTCCACCT 
GGGCCTCCTG GAGTTGTTGG ACCTCAGGGT CCAGCTGGAG AGACTGGCCC TAATGGTGAA 
CGTGGCCACC CTGGACCACC AGGTCCACCA GGAGAACAGG GACTTCCTGG TCCCTCAGGA 
AAGGAGGGAA CCAAAGGAGA CCCTGGACCC CCAGGAGGCC CTGGCAAAGA TGGGCCTCCT 
GGACTGAGGG GCTTCCCTGG TGAGAGAGGA CTGCCAGGAA CACCTGGAGG TGGAGGATTC 
AAAGGAAGCG AAGGACCTGC TGGACCTCCT GGACCGGCTG GCTCTCCTGG TGAGAGGGGG 
CAATCTGGTA CCGCTGGGCC TGTTGGACCT CCTGGAAGAC CAGGTCCTCA GGGACCTCCT 
GGACCTGCAG GAGAGAAAGG TGTTTCTGGT GAGAAAGGCC CAATTGGCCC AGCTGGTCGT 
GACGGACTAC AGGGTCCTGT GGGTCTGCCT GGCCCAGCTG GACCGCCCGG AGTACCTGGA 
GAAGATGGAG ATAAGGGGGA GGTTGGAGAA CACGGTCAGA AAGGTGCCAA GGGAGGGAAA 
GGAGAGCATG GTCCTCCAGG TCCACCTGGA CCAATGGGAC CAGTCGGCCA GCCTGGTGCT 
CCAGGTGCTG ATGGAGAGCT TGGGCCGAGG GGTCAGCAGG GACCTTTTGG AGCTAAAGGG 
GATGAAGGAA CCAGAGGATT CCCAGGAGCC CCAGGACCCA TTGGACTCCA GGGTTTGCCT 
GGGCCACCAG GTGAGAAGGG AGAAACCGGA GATGTTGGAC CTCTGGGACC TCCAGGCCCT 
CCAGGACCCC GTGGCCCTGC TGGACCCAAC GGTGCTGATG GCCCTCAAGG GCCTCCTGGT 
GGTTTGGGTA ATCCTGGACC TATCGGTGAG AAGGGAGAGC CTGGAGAGTC TGGACCCCCT 
GGAATTGGAG GGGAGCCTGG AAAGAAGGGC CCCAGAGGGG AACGTGGGGA GAAGGGGGAG 
GCTGGACAAC CAGGAACCGC TGGATCCACT GGAGGACGTG GACTACCTGG AGATGATGGA 
CCCAAAGGAA ACCCAGGTCC TGTTGGTTTC CCCGGTGATC CTGGTCCACC GGGTGAGGTT 
GGACCCAGAG GTCAAGACGG TGCCAAGGGA GAAAGAGGAG AAGATGGTGA AGCAGGAGAG 
TCTGGATCCC CTGGACCTCC CGGTGAGAAC GGACCCCCCG GCCCCCTAGG AAAGAGGGGT 
CCTGCTGGAA CAAGAGGACC AGAGGGACGC CAGGGAGAGA AGGGAACAAA GGGGGATCCA 
GGTGCTGTTG GCCCCCCAGG CAAAACAGGC CCTGTGGGTC CACAGGGAGT GCCAGGAAAA 
CCTGGAACAG AAGGCCTTAG AGGGCTTCCA GGATCAGTGG GTGAGCAAGG ATCTCCAGGA 
CCTGCAGGAC AGAAAGGACC ACCTGGTCCT ATTGGACCTC CCGGTTTACC TGGTCTGCGT 
GGAGATTCTG GCGGCAAAGG AGAAAAGGGA CACCCGGGTC TTATCGGTCT CATTGGACCC 
CCAGGAGAAC AGGGTGAGAA GGGAGACAGA GGCATGCCTG GGCCCCATGG ATCCAATGGA 
CCAAAAGGAG AGACTGGAAT GCCTGGAGGC ACAGGACCTA TCGGCCCCTC TGGTCCTCCT 
GGTCTGCCTG GTCCTCAAGG TATTAAAGGA GCCAAAGGTT CTTCTGGTGG AGCTGGTCCA 
AAAGGAGAAA AAGGAGTACA AGGACCCCCT GGACTTCCTG GCCCACCAGG GGAGGTTATT 
CAGCCACTAC CCATCCAAAG GAGTCCCAAG TCCAAGCGGT CCATTGATGC CAGCCAGCTA 
CTTCCTGAGA ATGACATGCC TGCATCCGAT GCCACCGGGA CAGAATTCCT GATGGGTAGT 
GAAGGCATGG AGGAGATCTT TGGCTCCCTC AACTCGCTTC GGCAGGAGAT TGAGACCATG 
CGCTTCCCTC TGGGCACCCA GGACAGCCCC GGCCGCACCT GCCAAGACCT GCACCTCAGT 
CAGCCAGAAC TCAAAGATGG AGAATACTGG ATTGACCCCA ACCAGGGCTG CTCCAGAGAC 
GCCTTCAAGG TCTTCTGTAA CTTTACTAAG GGGGCAGAGA CGTGCCTCTA CCCCAGCCAG 
AGCATCAGCA CGGTGAAGAT GAATTCCTGG AACAAAGAGA CTCCAGGTTC CTGGTACAGT 
CAGTTCTCCA CTGGCAGTAA GTTTTCATAC GTGGACTCCA ACGGCGAACC TGTGGGTGTG 
GTTCAGTTGG GTTTCCTTCG TCTCTTGAGC GTGCAGGCCC ACCAGACTCT CACATACCAC 
TGCCACCGCT CGGTAGCGTG GGCTGACCGC AACGCCAAGA ACACATACAG CCGAGCTCTG 
CACTTCAGGG GTGCCAACGA CGAGGATCTA AGCTATGAGA CCAATCCATA CGTCAAGGCT 
TTGGTTGATG GCTGCTCTTA TCGTAAGGGC TTTGACCGGA CAGTCCTGGA GATCAACACA 
CCACAGGTGG AACATCTTCC TCTGCTGGAT ATCAAGGTGT CAGACTTTGG GGAGAGCAAC 
CAGCAGTTTG GCTTTGAAGT GGGACCTGTC TGTTTCCAAG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615667.2
CDS	408..6872
Translation	MDTGDCPCRKKRRPWRMDFSSFALVTVMLIVCQTALVRADPVDVLRALQVPSLPEGVKKVPGFCTSRRSSSPDHAYRITKKAQISAPTKQLFSGRFPENFSIMALVKAQAGLQAFLLSIYSEQGIQQLGIEMGRSPVFLYEDQNGKPAPEDYPLFRGVNLADGKWHRIALSVSKKNVTLLLDCKKKMTRPLPRSNSAEVDTNGITVFGARLLDEEVFQGDIQQLLIASNPQAAYDFCEHYSPDCDSPLPKTQAQDPNTYKKQLNGKGAPTKSTAVKAKPTPAKPAKSGSYKLVVKSPMPIKAAKSTAKPFKGRPTAVEILLSKINPITPSKSTISLIKNGNGKSAAAKTNGLAKANNNGNGRATGAKANGNGKADSKPIAQARVSGNGKVVVEKKVIINAKSSAEKKSSGNGKVTIVTKSNGAAEKKTSGSGKATIEVKEIKVKVHGKPENKVAGEAKANGNGKIITVVEKKFTNGAANRAKIETTTRAKTEKVIKVEKIEKTTLSTAKSATKLDASKAAKPTKATKSDATTLTKATKAAAAAGKTQARAEVKEVTQVKTVVTKVPLVKTTVSKPAKNVVERVTMPRKVASTSMPVRPTPPRPTKPKMAVDINVKSQHKPFPPFGKTALSGTTKKVTNGYQQDVDTEYSEAGHTPTERDYYYEETVPLPEGEVVGQEELAVQPQVEPVLEGEEVHSTKAVGEGEEAFTEEYVTGDVTLKEYDYSYRDYNEPLVETGEVDANMGPALSAVTDEGGAGVRGQKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPSGPPGSVGDPGERGPPGKAGLPGADGVPGPPGTSVMLPFRFGQSGGDKGPAVSAQEAQAAAILSQARMALKGPPGPMGFTGRPGPLGNPGSPGLKGESGDPGPQGPRGSLGLMGPPGKSGRRGRAGADGARGMPGESGSKGDRGFDGLPGLPGDKGHRGDSGPMGPPGSQGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGIPGPPGVRGSDGPHGPKGNLGPQGEPGPPGQQGTPGTQGMPGPQGHTGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGPIGYPGPRGLKGGQGIRGLKGHKGEKGEDGFPGIKGDFGIKGERGEIGVPGPRGEDGPEGPKGRFGPPGETGPLGAVGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGSNGEKGTRGLTGKSGPRGQRGPTGPRGQRGPRGATGKLGPKGTSGSDGPPGPPGERGLPGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKMGPPGPPGVVGPQGPAGETGPNGERGHPGPPGPPGEQGLPGPSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGGGGFKGSEGPAGPPGPAGSPGERGQSGTAGPVGPPGRPGPQGPPGPAGEKGVSGEKGPIGPAGRDGLQGPVGLPGPAGPPGVPGEDGDKGEVGEHGQKGAKGGKGEHGPPGPPGPMGPVGQPGAPGADGELGPRGQQGPFGAKGDEGTRGFPGAPGPIGLQGLPGPPGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGIGGEPGKKGPRGERGEKGEAGQPGTAGSTGGRGLPGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGERGEDGEAGESGSPGPPGENGPPGPLGKRGPAGTRGPEGRQGEKGTKGDPGAVGPPGKTGPVGPQGVPGKPGTEGLRGLPGSVGEQGSPGPAGQKGPPGPIGPPGLPGLRGDSGGKGEKGHPGLIGLIGPPGEQGEKGDRGMPGPHGSNGPKGETGMPGGTGPIGPSGPPGLPGPQGIKGAKGSSGGAGPKGEKGVQGPPGLPGPPGEVIQPLPIQRSPKSKRSIDASQLLPENDMPASDATGTEFLMGSEGMEEIFGSLNSLRQEIETMRFPLGTQDSPGRTCQDLHLSQPELKDGEYWIDPNQGCSRDAFKVFCNFTKGAETCLYPSQSISTVKMNSWNKETPGSWYSQFSTGSKFSYVDSNGEPVGVVQLGFLRLLSVQAHQTLTYHCHRSVAWADRNAKNTYSRALHFRGANDEDLSYETNPYVKALVDGCSYRKGFDRTVLEINTPQVEHLPLLDIKVSDFGESNQQFGFEVGPVCFQG

Target ORF information:

RefSeq Version	XM_011617365.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617365.2

ORF Insert Sequence:

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGAC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATAAG 
AAGCAGCTCA ATGGAAAAGG AGCCCCCACT AAATCCACTG CTGTTAAAGC AAAACCTACC 
CCAGCAAAGC CAGCCAAGTC TGGATCATAT AAGCTTGTAG TCAAGTCCCC TATGCCCATC 
AAAGCAGCAA AGTCTACAGC CAAGCCATTT AAAGGCAGGC CCACTGCAGT AGAGATCCTT 
CTCTCTAAGA TAAACCCAAT AACACCATCT AAATCCACAA TTTCCTTGAT TAAAAATGGC 
AACGGTAAAT CAGCTGCAGC AAAGACAAAT GGATTGGCCA AAGCCAACAA CAATGGAAAT 
GGAAGAGCAA CAGGCGCTAA AGCAAATGGC AATGGCAAAG CTGATTCTAA GCCCATAGCT 
CAGGCCAGAG TGAGCGGAAA TGGCAAAGTT GTAGTGGAGA AAAAAGTCAT CATAAATGCC 
AAATCTTCAG CAGAAAAGAA ATCCAGCGGA AATGGAAAAG TCACCATTGT AACCAAGAGT 
AATGGTGCAG CAGAGAAGAA AACCAGTGGT AGTGGCAAAG CTACCATTGA GGTTAAAGAA 
ATTAAAGTCA AAGTTCATGG AAAACCTGAA AATAAAGTTG CAGGTGAGGC CAAGGCTAAC 
GGCAATGGTA AAATTATCAC TGTGGTAGAA AAGAAATTCA CCAATGGTGC TGCGAATCGG 
GCAAAGATTG AAACCACTAC AAGAGCAAAG ACGGAGAAAG TTATCAAAGT TGAAAAGATA 
GAAAAGACTA CGCTCAGTAC AGCTAAATCA GCCACAAAAC TAGATGCTTC TAAAGCAGCC 
AAACCCACAA AAGCCACAAA ATCTGATGCT ACAACATTAA CAAAGGCCAC CAAAGCAGCG 
GCAGCAGCAG GAAAGACCCA AGCAAGGGCA GAGGTCAAAG AAGTCACCCA AGTTAAAACT 
GTTGTCACCA AAGTCCCTCT GGTCAAAACC ACTGTGAGCA AACCAGCGAA GAATGTGGTG 
GAGAGAGTTA CGATGCCCAG GAAAGTAGCC TCGACGTCCA TGCCTGTGAG GCCCACTCCA 
CCCAGACCTA CGAAACCCAA GATGGCGGTG GACATTAATG TCAAATCCCA GCACAAACCT 
TTCCCACCAT TTGGCAAGAC TGCGTTGAGT GGAACTACGA AGAAAGTTAC CAACGGTTAT 
CAGCAGGATG TAGACACTGA ATATTCCGAG GCTGGCCACA CCCCTACAGA GAGAGATTAT 
TACTATGAGG AGACGGTACC ACTGCCAGAG GGTGAGGTGG TTGGGCAAGA GGAACTCGCC 
GTACAGCCCC AGGTGGAGCC TGTGTTGGAG GGAGAAGAGG TGCATTCGAC CAAGGCTGTC 
GGTGAGGGAG AGGAGGCCTT CACAGAGGAG TATGTGACTG GAGATGTGAC CCTTAAGGAA 
TACGACTATT CCTACAGGGA CTACAATGAG CCATTAGTGG AGACTGGAGA AGTCGATGCC 
AACATGGGCC CCGCCCTCTC TGCTGTGACA GACGAGGGAG GCGCTGGGGT GAGAGGCCAG 
AAAGGAGAGA AAGGAGAACC TGCTGTTTTG GAGCCTGGGA TGCTGATTGA AGGACCACCA 
GGACCTGAGG GACCTGCTGG CCTACCTGGT CCTTCTGGCC CTTCTGGACC TCCTGGCTCT 
GTGGGTGACC CAGGAGAGAG GGGCCCTCCT GGTAAGGCTG GTCTTCCTGG AGCTGATGGA 
GTCCCTGGAC CTCCTGGGAC CTCTGTTATG CTGCCTTTCC GTTTTGGTCA GAGTGGAGGA 
GATAAAGGTC CTGCTGTTTC TGCTCAGGAA GCTCAGGCAG CAGCCATCTT GTCTCAGGCC 
AGGATGGCTC TTAAAGGACC TCCTGGTCCA ATGGGATTCA CTGGGCGCCC CGGACCCTTG 
GGAAATCCTG GAAGTCCTGG TCTGAAAGGA GAGAGTGGAG ACCCTGGACC CCAGGGCCCG 
AGGGGATCTC TAGGCTTGAT GGGACCTCCA GGCAAATCAG GAAGAAGAGG CCGCGCTGGA 
GCCGACGGTG CCAGAGGAAT GCCTGGAGAG TCTGGTTCTA AGGGTGATCG TGGTTTTGAT 
GGTCTTCCTG GTTTACCTGG AGACAAAGGA CACAGGGGTG ACTCGGGACC AATGGGACCT 
CCAGGATCTC AGGGAGAGGA TGGAGAAAGG GGAGACGACG GAGACGTTGG ACCCCGGGGT 
CTTCCAGGTG AACCAGGACC TCGTGGTTTG CTTGGACCTA AAGGTCCTCC TGGAATCCCA 
GGCCCTCCTG GTGTACGAGG AAGTGACGGA CCCCACGGTC CAAAAGGAAA CTTGGGTCCT 
CAAGGTGAGC CAGGACCCCC TGGTCAGCAG GGAACCCCAG GAACTCAGGG AATGCCAGGA 
CCTCAAGGAC ATACTGGACC TCCAGGAGAA AAGGGTCCAA CAGGAAAACC GGGTTTACCA 
GGAATGCCTG GAGCTGATGG CCCTCCTGGT CACCCAGGAA AGGAAGGCCC ATCAGGCACC 
AAAGGAAACC AGGGTCCCAG TGGTCCCCAG GGGCCTATTG GCTATCCTGG TCCTCGTGGC 
CTCAAGGGCG GTCAAGGAAT CCGGGGATTG AAGGGTCACA AAGGAGAGAA GGGAGAAGAT 
GGTTTCCCTG GAATTAAGGG AGACTTTGGG ATCAAGGGTG AGAGGGGTGA GATTGGAGTA 
CCAGGTCCTA GAGGAGAGGA CGGTCCAGAG GGGCCAAAAG GTCGTTTTGG TCCCCCTGGT 
GAAACTGGAC CACTCGGAGC AGTAGGAGAG AAGGGTAAAC TTGGTGTACC GGGACTTCCT 
GGATATCCTG GAAGACAAGG ACCAAAGGGA TCTCTTGGAT TCCCAGGATT CCCTGGATCC 
AATGGCGAGA AGGGAACAAG GGGTCTCACT GGCAAATCAG GACCAAGAGG ACAAAGAGGA 
CCAACGGGCC CCAGAGGGCA GAGGGGACCG AGGGGCGCAA CTGGCAAACT GGGGCCAAAG 
GGGACTTCGG GAAGTGATGG CCCCCCTGGT CCTCCTGGAG AAAGGGGCTT GCCTGGGCCT 
CAGGGAGCCA ATGGGTTCCC CGGACCTAAA GGGCCACCGG GGCCACCAGG GAAGGACGGG 
CTGCCTGGAC ACCCTGGGCA GAGAGGAGAA GTGGGTTTCC AAGGTAAAAT GGGTCCACCT 
GGGCCTCCTG GAGTTGTTGG ACCTCAGGGT CCAGCTGGAG AGACTGGCCC TAATGGTGAA 
CGTGGCCACC CTGGACCACC AGGTCCACCA GGAGAACAGG GACTTCCTGG TCCCTCAGGA 
AAGGAGGGAA CCAAAGGAGA CCCTGGACCC CCAGGAGGCC CTGGCAAAGA TGGGCCTCCT 
GGACTGAGGG GCTTCCCTGG TGAGAGAGGA CTGCCAGGAA CACCTGGAGG TGGAGGATTC 
AAAGGAAGCG AAGGACCTGC TGGACCTCCT GGACCGGCTG GCTCTCCTGG TGAGAGGGGG 
CAATCTGGTA CCGCTGGGCC TGTTGGACCT CCTGGAAGAC CAGGTCCTCA GGGACCTCCT 
GGACCTGCAG GAGAGAAAGG TGTTTCTGGT GAGAAAGGCC CAATTGGCCC AGCTGGTCGT 
GACGGACTAC AGGGTCCTGT GGGTCTGCCT GGCCCAGCTG GACCGCCCGG AGTACCTGGA 
GAAGATGGAG ATAAGGGGGA GGTTGGAGAA CACGGTCAGA AAGGTGCCAA GGGAGGGAAA 
GGAGAGCATG GTCCTCCAGG TCCACCTGGA CCAATGGGAC CAGTCGGCCA GCCTGGTGCT 
CCAGGTGCTG ATGGAGAGCT TGGGCCGAGG GGTCAGCAGG GACCTTTTGG AGCTAAAGGG 
GATGAAGGAA CCAGAGGATT CCCAGGAGCC CCAGGACCCA TTGGACTCCA GGGTTTGCCT 
GGGCCACCAG GTGAGAAGGG AGAAACCGGA GATGTTGGAC CTCTGGGACC TCCAGGCCCT 
CCAGGACCCC GTGGCCCTGC TGGACCCAAC GGTGCTGATG GCCCTCAAGG GCCTCCTGGT 
GGTTTGGGTA ATCCTGGACC TATCGGTGAG AAGGGAGAGC CTGGAGAGTC TGGACCCCCT 
GGAATTGGAG GGGAGCCTGG AAAGAAGGGC CCCAGAGGGG AACGTGGGGA GAAGGGGGAG 
GCTGGACAAC CAGGAACCGC TGGATCCACT GGAGGACGTG GACTACCTGG AGATGATGGA 
CCCAAAGGAA ACCCAGGTCC TGTTGGTTTC CCCGGTGATC CTGGTCCACC GGGTGAGGTT 
GGACCCAGAG GTCAAGACGG TGCCAAGGGA GAAAGAGGAG AAGATGGTGA AGCAGGAGAG 
TCTGGATCCC CTGGACCTCC CGGTGAGAAC GGACCCCCCG GCCCCCTAGG AAAGAGGGGT 
CCTGCTGGAA CAAGAGGACC AGAGGGACGC CAGGGAGAGA AGGGAACAAA GGGGGATCCA 
GGTGCTGTTG GCCCCCCAGG CAAAACAGGC CCTGTGGGTC CACAGGGAGT GCCAGGAAAA 
CCTGGAACAG AAGGCCTTAG AGGGCTTCCA GGATCAGTGG GTGAGCAAGG ATCTCCAGGA 
CCTGCAGGAC AGAAAGGACC ACCTGGTCCT ATTGGACCTC CCGGTTTACC TGGTCTGCGT 
GGAGATTCTG GCGGCAAAGG AGAAAAGGGA CACCCGGGTC TTATCGGTCT CATTGGACCC 
CCAGGAGAAC AGGGTGAGAA GGGAGACAGA GGCATGCCTG GGCCCCATGG ATCCAATGGA 
CCAAAAGGAG AGACTGGAAT GCCTGGAGGC ACAGGACCTA TCGGCCCCTC TGGTCCTCCT 
GGTCTGCCTG GTCCTCAAGG TATTAAAGGA GCCAAAGGTT CTTCTGGTGG AGCTGGTCCA 
AAAGGAGAAA AAGGAGTACA AGGACCCCCT GGACTTCCTG GCCCACCAGG GGAGGTTATT 
CAGCCACTAC CCATCCAAAG GAGTCCCAAG TCCAAGCGGT CCATTGATGC CAGCCAGCTA 
CTTCCTGAGA ATGACATGCC TGCATCCGAT GCCACCGGGA CAGAATTCCT GATGGGTAGT 
GAAGGCATGG AGGAGATCTT TGGCTCCCTC AACTCGCTTC GGCAGGAGAT TGAGACCATG 
CGCTTCCCTC TGGGCACCCA GGACAGCCCC GGCCGCACCT GCCAAGACCT GCACCTCAGT 
CAGCCAGAAC TCAAAGATGG AGAATACTGG ATTGACCCCA ACCAGGGCTG CTCCAGAGAC 
GCCTTCAAGG TCTTCTGTAA CTTTACTAAG GGGGCAGAGA CGTGCCTCTA CCCCAGCCAG 
AGCATCAGCA CGGTGAAGAT GAATTCCTGG AACAAAGAGA CTCCAGGTTC CTGGTACAGT 
CAGTTCTCCA CTGGCAGTAA GTTTTCATAC GTGGACTCCA ACGGCGAACC TGTGGGTGTG 
GTTCAGTTGG GTTTCCTTCG TCTCTTGAGC GTGCAGGCCC ACCAGACTCT CACATACCAC 
TGCCACCGCT CGGTAGCGTG GGCTGACCGC AACGCCAAGA ACACATACAG CCGAGCTCTG 
CACTTCAGGG GTGCCAACGA CGAGGATCTA AGCTATGAGA CCAATCCATA CGTCAAGGCT 
TTGGTTGATG GCTGCTCTTA TCGTAAGGGC TTTGACCGGA CAGTCCTGGA GATCAACACA 
CCACAGGTGG AACATCTTCC TCTGCTGGAT ATCAAGGTGT CAGACTTTGG GGAGAGCAAC 
CAGCAGTTTG GCTTTGAAGT GGGACCTGTC TGTTTCCAAG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101066527 ( XM_011617366.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011615668.2 Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X2, mRNA.

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CloneID	OTo45738
Clone ID Related Accession (Same CDS sequence)	XM_011617366.2
Accession Version	XM_011617366.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	collagen alpha-1(XI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042296.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011617366.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGAC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATAAG 
AAGCAGCTCA ATGGAAAAGG AGCCCCCACT AAATCCACTG CTGTTAAAGC AAAACCTACC 
CCAGCAAAGC CAGCCAAGTC TGGATCATAT AAGCTTGTAG TCAAGTCCCC TATGCCCATC 
AAAGCAGCAA AGTCTACAGC CAAGCCATTT AAAGGCAGGC CCACTGCAGT AGAGATCCTT 
CTCTCTAAGA TAAACCCAAT AACACCATCT AAATCCACAA TTTCCTTGAT TAAAAATGGC 
AACGGTAAAT CAGCTGCAGC AAAGACAAAT GGATTGGCCA AAGCCAACAA CAATGGAAAT 
GGAAGAGCAA CAGGCGCTAA AGCAAATGGC AATGGCAAAG CTGATTCTAA GCCCATAGCT 
CAGGCCAGAG TGAGCGGAAA TGGCAAAGTT GTAGTGGAGA AAAAAGTCAT CATAAATGCC 
AAATCTTCAG CAGAAAAGAA ATCCAGCGGA AATGGAAAAG TCACCATTGT AACCAAGAGT 
AATGGTGCAG CAGAGAAGAA AACCAGTGGT AGTGGCAAAG CTACCATTGA GGTTAAAGAA 
ATTAAAGTCA AAGTTCATGG AAAACCTGAA AATAAAGTTG CAGGTGAGGC CAAGGCTAAC 
GGCAATGGTA AAATTATCAC TGTGGTAGAA AAGAAATTCA CCAATGGTGC TGCGAATCGG 
GCAAAGATTG AAACCACTAC AAGAGCAAAG ACGGAGAAAG TTATCAAAGT TGAAAAGATA 
GAAAAGACTA CGCTCAGTAC AGCTAAATCA GCCACAAAAC TAGATGCTTC TAAAGCAGCC 
AAACCCACAA AAGCCACAAA ATCTGATGCT ACAACATTAA CAAAGGCCAC CAAAGCAGCG 
GCAGCAGCAG GAAAGACCCA AGCAAGGGCA GAGGTCAAAG AAGTCACCCA AGTTAAAACT 
GTTGTCACCA AAGTCCCTCT GGTCAAAACC ACTGTGAGCA AACCAGCGAA GAATGTGGTG 
GAGAGAGTTA CGATGCCCAG GAAAGTAGCC TCGACGTCCA TGCCTGTGAG GCCCACTCCA 
CCCAGACCTA CGAAACCCAA GATGGCGGTG GACATTAATG TCAAATCCCA GCACAAACCT 
TTCCCACCAT TTGGCAAGAC TGCGTTGAGT GGAACTACGA AGAAAGTTAC CAACGGTTAT 
CAGCAGCCCC AGGTGGAGCC TGTGTTGGAG GGAGAAGAGG TGCATTCGAC CAAGGCTGTC 
GGTGAGGGAG AGGAGGCCTT CACAGAGGAG TATGTGACTG GAGATGTGAC CCTTAAGGAA 
TACGACTATT CCTACAGGGA CTACAATGAG CCATTAGTGG AGACTGGAGA AGTCGATGCC 
AACATGGGCC CCGCCCTCTC TGCTGTGACA GACGAGGGAG GCGCTGGGGT GAGAGGCCAG 
AAAGGAGAGA AAGGAGAACC TGCTGTTTTG GAGCCTGGGA TGCTGATTGA AGGACCACCA 
GGACCTGAGG GACCTGCTGG CCTACCTGGT CCTTCTGGCC CTTCTGGACC TCCTGGCTCT 
GTGGGTGACC CAGGAGAGAG GGGCCCTCCT GGTAAGGCTG GTCTTCCTGG AGCTGATGGA 
GTCCCTGGAC CTCCTGGGAC CTCTGTTATG CTGCCTTTCC GTTTTGGTCA GAGTGGAGGA 
GATAAAGGTC CTGCTGTTTC TGCTCAGGAA GCTCAGGCAG CAGCCATCTT GTCTCAGGCC 
AGGATGGCTC TTAAAGGACC TCCTGGTCCA ATGGGATTCA CTGGGCGCCC CGGACCCTTG 
GGAAATCCTG GAAGTCCTGG TCTGAAAGGA GAGAGTGGAG ACCCTGGACC CCAGGGCCCG 
AGGGGATCTC TAGGCTTGAT GGGACCTCCA GGCAAATCAG GAAGAAGAGG CCGCGCTGGA 
GCCGACGGTG CCAGAGGAAT GCCTGGAGAG TCTGGTTCTA AGGGTGATCG TGGTTTTGAT 
GGTCTTCCTG GTTTACCTGG AGACAAAGGA CACAGGGGTG ACTCGGGACC AATGGGACCT 
CCAGGATCTC AGGGAGAGGA TGGAGAAAGG GGAGACGACG GAGACGTTGG ACCCCGGGGT 
CTTCCAGGTG AACCAGGACC TCGTGGTTTG CTTGGACCTA AAGGTCCTCC TGGAATCCCA 
GGCCCTCCTG GTGTACGAGG AAGTGACGGA CCCCACGGTC CAAAAGGAAA CTTGGGTCCT 
CAAGGTGAGC CAGGACCCCC TGGTCAGCAG GGAACCCCAG GAACTCAGGG AATGCCAGGA 
CCTCAAGGAC ATACTGGACC TCCAGGAGAA AAGGGTCCAA CAGGAAAACC GGGTTTACCA 
GGAATGCCTG GAGCTGATGG CCCTCCTGGT CACCCAGGAA AGGAAGGCCC ATCAGGCACC 
AAAGGAAACC AGGGTCCCAG TGGTCCCCAG GGGCCTATTG GCTATCCTGG TCCTCGTGGC 
CTCAAGGGCG GTCAAGGAAT CCGGGGATTG AAGGGTCACA AAGGAGAGAA GGGAGAAGAT 
GGTTTCCCTG GAATTAAGGG AGACTTTGGG ATCAAGGGTG AGAGGGGTGA GATTGGAGTA 
CCAGGTCCTA GAGGAGAGGA CGGTCCAGAG GGGCCAAAAG GTCGTTTTGG TCCCCCTGGT 
GAAACTGGAC CACTCGGAGC AGTAGGAGAG AAGGGTAAAC TTGGTGTACC GGGACTTCCT 
GGATATCCTG GAAGACAAGG ACCAAAGGGA TCTCTTGGAT TCCCAGGATT CCCTGGATCC 
AATGGCGAGA AGGGAACAAG GGGTCTCACT GGCAAATCAG GACCAAGAGG ACAAAGAGGA 
CCAACGGGCC CCAGAGGGCA GAGGGGACCG AGGGGCGCAA CTGGCAAACT GGGGCCAAAG 
GGGACTTCGG GAAGTGATGG CCCCCCTGGT CCTCCTGGAG AAAGGGGCTT GCCTGGGCCT 
CAGGGAGCCA ATGGGTTCCC CGGACCTAAA GGGCCACCGG GGCCACCAGG GAAGGACGGG 
CTGCCTGGAC ACCCTGGGCA GAGAGGAGAA GTGGGTTTCC AAGGTAAAAT GGGTCCACCT 
GGGCCTCCTG GAGTTGTTGG ACCTCAGGGT CCAGCTGGAG AGACTGGCCC TAATGGTGAA 
CGTGGCCACC CTGGACCACC AGGTCCACCA GGAGAACAGG GACTTCCTGG TCCCTCAGGA 
AAGGAGGGAA CCAAAGGAGA CCCTGGACCC CCAGGAGGCC CTGGCAAAGA TGGGCCTCCT 
GGACTGAGGG GCTTCCCTGG TGAGAGAGGA CTGCCAGGAA CACCTGGAGG TGGAGGATTC 
AAAGGAAGCG AAGGACCTGC TGGACCTCCT GGACCGGCTG GCTCTCCTGG TGAGAGGGGG 
CAATCTGGTA CCGCTGGGCC TGTTGGACCT CCTGGAAGAC CAGGTCCTCA GGGACCTCCT 
GGACCTGCAG GAGAGAAAGG TGTTTCTGGT GAGAAAGGCC CAATTGGCCC AGCTGGTCGT 
GACGGACTAC AGGGTCCTGT GGGTCTGCCT GGCCCAGCTG GACCGCCCGG AGTACCTGGA 
GAAGATGGAG ATAAGGGGGA GGTTGGAGAA CACGGTCAGA AAGGTGCCAA GGGAGGGAAA 
GGAGAGCATG GTCCTCCAGG TCCACCTGGA CCAATGGGAC CAGTCGGCCA GCCTGGTGCT 
CCAGGTGCTG ATGGAGAGCT TGGGCCGAGG GGTCAGCAGG GACCTTTTGG AGCTAAAGGG 
GATGAAGGAA CCAGAGGATT CCCAGGAGCC CCAGGACCCA TTGGACTCCA GGGTTTGCCT 
GGGCCACCAG GTGAGAAGGG AGAAACCGGA GATGTTGGAC CTCTGGGACC TCCAGGCCCT 
CCAGGACCCC GTGGCCCTGC TGGACCCAAC GGTGCTGATG GCCCTCAAGG GCCTCCTGGT 
GGTTTGGGTA ATCCTGGACC TATCGGTGAG AAGGGAGAGC CTGGAGAGTC TGGACCCCCT 
GGAATTGGAG GGGAGCCTGG AAAGAAGGGC CCCAGAGGGG AACGTGGGGA GAAGGGGGAG 
GCTGGACAAC CAGGAACCGC TGGATCCACT GGAGGACGTG GACTACCTGG AGATGATGGA 
CCCAAAGGAA ACCCAGGTCC TGTTGGTTTC CCCGGTGATC CTGGTCCACC GGGTGAGGTT 
GGACCCAGAG GTCAAGACGG TGCCAAGGGA GAAAGAGGAG AAGATGGTGA AGCAGGAGAG 
TCTGGATCCC CTGGACCTCC CGGTGAGAAC GGACCCCCCG GCCCCCTAGG AAAGAGGGGT 
CCTGCTGGAA CAAGAGGACC AGAGGGACGC CAGGGAGAGA AGGGAACAAA GGGGGATCCA 
GGTGCTGTTG GCCCCCCAGG CAAAACAGGC CCTGTGGGTC CACAGGGAGT GCCAGGAAAA 
CCTGGAACAG AAGGCCTTAG AGGGCTTCCA GGATCAGTGG GTGAGCAAGG ATCTCCAGGA 
CCTGCAGGAC AGAAAGGACC ACCTGGTCCT ATTGGACCTC CCGGTTTACC TGGTCTGCGT 
GGAGATTCTG GCGGCAAAGG AGAAAAGGGA CACCCGGGTC TTATCGGTCT CATTGGACCC 
CCAGGAGAAC AGGGTGAGAA GGGAGACAGA GGCATGCCTG GGCCCCATGG ATCCAATGGA 
CCAAAAGGAG AGACTGGAAT GCCTGGAGGC ACAGGACCTA TCGGCCCCTC TGGTCCTCCT 
GGTCTGCCTG GTCCTCAAGG TATTAAAGGA GCCAAAGGTT CTTCTGGTGG AGCTGGTCCA 
AAAGGAGAAA AAGGAGTACA AGGACCCCCT GGACTTCCTG GCCCACCAGG GGAGGTTATT 
CAGCCACTAC CCATCCAAAG GAGTCCCAAG TCCAAGCGGT CCATTGATGC CAGCCAGCTA 
CTTCCTGAGA ATGACATGCC TGCATCCGAT GCCACCGGGA CAGAATTCCT GATGGGTAGT 
GAAGGCATGG AGGAGATCTT TGGCTCCCTC AACTCGCTTC GGCAGGAGAT TGAGACCATG 
CGCTTCCCTC TGGGCACCCA GGACAGCCCC GGCCGCACCT GCCAAGACCT GCACCTCAGT 
CAGCCAGAAC TCAAAGATGG AGAATACTGG ATTGACCCCA ACCAGGGCTG CTCCAGAGAC 
GCCTTCAAGG TCTTCTGTAA CTTTACTAAG GGGGCAGAGA CGTGCCTCTA CCCCAGCCAG 
AGCATCAGCA CGGTGAAGAT GAATTCCTGG AACAAAGAGA CTCCAGGTTC CTGGTACAGT 
CAGTTCTCCA CTGGCAGTAA GTTTTCATAC GTGGACTCCA ACGGCGAACC TGTGGGTGTG 
GTTCAGTTGG GTTTCCTTCG TCTCTTGAGC GTGCAGGCCC ACCAGACTCT CACATACCAC 
TGCCACCGCT CGGTAGCGTG GGCTGACCGC AACGCCAAGA ACACATACAG CCGAGCTCTG 
CACTTCAGGG GTGCCAACGA CGAGGATCTA AGCTATGAGA CCAATCCATA CGTCAAGGCT 
TTGGTTGATG GCTGCTCTTA TCGTAAGGGC TTTGACCGGA CAGTCCTGGA GATCAACACA 
CCACAGGTGG AACATCTTCC TCTGCTGGAT ATCAAGGTGT CAGACTTTGG GGAGAGCAAC 
CAGCAGTTTG GCTTTGAAGT GGGACCTGTC TGTTTCCAAG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615668.2
CDS	408..6752
Translation	MDTGDCPCRKKRRPWRMDFSSFALVTVMLIVCQTALVRADPVDVLRALQVPSLPEGVKKVPGFCTSRRSSSPDHAYRITKKAQISAPTKQLFSGRFPENFSIMALVKAQAGLQAFLLSIYSEQGIQQLGIEMGRSPVFLYEDQNGKPAPEDYPLFRGVNLADGKWHRIALSVSKKNVTLLLDCKKKMTRPLPRSNSAEVDTNGITVFGARLLDEEVFQGDIQQLLIASNPQAAYDFCEHYSPDCDSPLPKTQAQDPNTYKKQLNGKGAPTKSTAVKAKPTPAKPAKSGSYKLVVKSPMPIKAAKSTAKPFKGRPTAVEILLSKINPITPSKSTISLIKNGNGKSAAAKTNGLAKANNNGNGRATGAKANGNGKADSKPIAQARVSGNGKVVVEKKVIINAKSSAEKKSSGNGKVTIVTKSNGAAEKKTSGSGKATIEVKEIKVKVHGKPENKVAGEAKANGNGKIITVVEKKFTNGAANRAKIETTTRAKTEKVIKVEKIEKTTLSTAKSATKLDASKAAKPTKATKSDATTLTKATKAAAAAGKTQARAEVKEVTQVKTVVTKVPLVKTTVSKPAKNVVERVTMPRKVASTSMPVRPTPPRPTKPKMAVDINVKSQHKPFPPFGKTALSGTTKKVTNGYQQPQVEPVLEGEEVHSTKAVGEGEEAFTEEYVTGDVTLKEYDYSYRDYNEPLVETGEVDANMGPALSAVTDEGGAGVRGQKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPSGPPGSVGDPGERGPPGKAGLPGADGVPGPPGTSVMLPFRFGQSGGDKGPAVSAQEAQAAAILSQARMALKGPPGPMGFTGRPGPLGNPGSPGLKGESGDPGPQGPRGSLGLMGPPGKSGRRGRAGADGARGMPGESGSKGDRGFDGLPGLPGDKGHRGDSGPMGPPGSQGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGIPGPPGVRGSDGPHGPKGNLGPQGEPGPPGQQGTPGTQGMPGPQGHTGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGPIGYPGPRGLKGGQGIRGLKGHKGEKGEDGFPGIKGDFGIKGERGEIGVPGPRGEDGPEGPKGRFGPPGETGPLGAVGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGSNGEKGTRGLTGKSGPRGQRGPTGPRGQRGPRGATGKLGPKGTSGSDGPPGPPGERGLPGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKMGPPGPPGVVGPQGPAGETGPNGERGHPGPPGPPGEQGLPGPSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGGGGFKGSEGPAGPPGPAGSPGERGQSGTAGPVGPPGRPGPQGPPGPAGEKGVSGEKGPIGPAGRDGLQGPVGLPGPAGPPGVPGEDGDKGEVGEHGQKGAKGGKGEHGPPGPPGPMGPVGQPGAPGADGELGPRGQQGPFGAKGDEGTRGFPGAPGPIGLQGLPGPPGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGIGGEPGKKGPRGERGEKGEAGQPGTAGSTGGRGLPGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGERGEDGEAGESGSPGPPGENGPPGPLGKRGPAGTRGPEGRQGEKGTKGDPGAVGPPGKTGPVGPQGVPGKPGTEGLRGLPGSVGEQGSPGPAGQKGPPGPIGPPGLPGLRGDSGGKGEKGHPGLIGLIGPPGEQGEKGDRGMPGPHGSNGPKGETGMPGGTGPIGPSGPPGLPGPQGIKGAKGSSGGAGPKGEKGVQGPPGLPGPPGEVIQPLPIQRSPKSKRSIDASQLLPENDMPASDATGTEFLMGSEGMEEIFGSLNSLRQEIETMRFPLGTQDSPGRTCQDLHLSQPELKDGEYWIDPNQGCSRDAFKVFCNFTKGAETCLYPSQSISTVKMNSWNKETPGSWYSQFSTGSKFSYVDSNGEPVGVVQLGFLRLLSVQAHQTLTYHCHRSVAWADRNAKNTYSRALHFRGANDEDLSYETNPYVKALVDGCSYRKGFDRTVLEINTPQVEHLPLLDIKVSDFGESNQQFGFEVGPVCFQG

Target ORF information:

RefSeq Version	XM_011617366.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617366.2

ORF Insert Sequence:

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGAC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATAAG 
AAGCAGCTCA ATGGAAAAGG AGCCCCCACT AAATCCACTG CTGTTAAAGC AAAACCTACC 
CCAGCAAAGC CAGCCAAGTC TGGATCATAT AAGCTTGTAG TCAAGTCCCC TATGCCCATC 
AAAGCAGCAA AGTCTACAGC CAAGCCATTT AAAGGCAGGC CCACTGCAGT AGAGATCCTT 
CTCTCTAAGA TAAACCCAAT AACACCATCT AAATCCACAA TTTCCTTGAT TAAAAATGGC 
AACGGTAAAT CAGCTGCAGC AAAGACAAAT GGATTGGCCA AAGCCAACAA CAATGGAAAT 
GGAAGAGCAA CAGGCGCTAA AGCAAATGGC AATGGCAAAG CTGATTCTAA GCCCATAGCT 
CAGGCCAGAG TGAGCGGAAA TGGCAAAGTT GTAGTGGAGA AAAAAGTCAT CATAAATGCC 
AAATCTTCAG CAGAAAAGAA ATCCAGCGGA AATGGAAAAG TCACCATTGT AACCAAGAGT 
AATGGTGCAG CAGAGAAGAA AACCAGTGGT AGTGGCAAAG CTACCATTGA GGTTAAAGAA 
ATTAAAGTCA AAGTTCATGG AAAACCTGAA AATAAAGTTG CAGGTGAGGC CAAGGCTAAC 
GGCAATGGTA AAATTATCAC TGTGGTAGAA AAGAAATTCA CCAATGGTGC TGCGAATCGG 
GCAAAGATTG AAACCACTAC AAGAGCAAAG ACGGAGAAAG TTATCAAAGT TGAAAAGATA 
GAAAAGACTA CGCTCAGTAC AGCTAAATCA GCCACAAAAC TAGATGCTTC TAAAGCAGCC 
AAACCCACAA AAGCCACAAA ATCTGATGCT ACAACATTAA CAAAGGCCAC CAAAGCAGCG 
GCAGCAGCAG GAAAGACCCA AGCAAGGGCA GAGGTCAAAG AAGTCACCCA AGTTAAAACT 
GTTGTCACCA AAGTCCCTCT GGTCAAAACC ACTGTGAGCA AACCAGCGAA GAATGTGGTG 
GAGAGAGTTA CGATGCCCAG GAAAGTAGCC TCGACGTCCA TGCCTGTGAG GCCCACTCCA 
CCCAGACCTA CGAAACCCAA GATGGCGGTG GACATTAATG TCAAATCCCA GCACAAACCT 
TTCCCACCAT TTGGCAAGAC TGCGTTGAGT GGAACTACGA AGAAAGTTAC CAACGGTTAT 
CAGCAGCCCC AGGTGGAGCC TGTGTTGGAG GGAGAAGAGG TGCATTCGAC CAAGGCTGTC 
GGTGAGGGAG AGGAGGCCTT CACAGAGGAG TATGTGACTG GAGATGTGAC CCTTAAGGAA 
TACGACTATT CCTACAGGGA CTACAATGAG CCATTAGTGG AGACTGGAGA AGTCGATGCC 
AACATGGGCC CCGCCCTCTC TGCTGTGACA GACGAGGGAG GCGCTGGGGT GAGAGGCCAG 
AAAGGAGAGA AAGGAGAACC TGCTGTTTTG GAGCCTGGGA TGCTGATTGA AGGACCACCA 
GGACCTGAGG GACCTGCTGG CCTACCTGGT CCTTCTGGCC CTTCTGGACC TCCTGGCTCT 
GTGGGTGACC CAGGAGAGAG GGGCCCTCCT GGTAAGGCTG GTCTTCCTGG AGCTGATGGA 
GTCCCTGGAC CTCCTGGGAC CTCTGTTATG CTGCCTTTCC GTTTTGGTCA GAGTGGAGGA 
GATAAAGGTC CTGCTGTTTC TGCTCAGGAA GCTCAGGCAG CAGCCATCTT GTCTCAGGCC 
AGGATGGCTC TTAAAGGACC TCCTGGTCCA ATGGGATTCA CTGGGCGCCC CGGACCCTTG 
GGAAATCCTG GAAGTCCTGG TCTGAAAGGA GAGAGTGGAG ACCCTGGACC CCAGGGCCCG 
AGGGGATCTC TAGGCTTGAT GGGACCTCCA GGCAAATCAG GAAGAAGAGG CCGCGCTGGA 
GCCGACGGTG CCAGAGGAAT GCCTGGAGAG TCTGGTTCTA AGGGTGATCG TGGTTTTGAT 
GGTCTTCCTG GTTTACCTGG AGACAAAGGA CACAGGGGTG ACTCGGGACC AATGGGACCT 
CCAGGATCTC AGGGAGAGGA TGGAGAAAGG GGAGACGACG GAGACGTTGG ACCCCGGGGT 
CTTCCAGGTG AACCAGGACC TCGTGGTTTG CTTGGACCTA AAGGTCCTCC TGGAATCCCA 
GGCCCTCCTG GTGTACGAGG AAGTGACGGA CCCCACGGTC CAAAAGGAAA CTTGGGTCCT 
CAAGGTGAGC CAGGACCCCC TGGTCAGCAG GGAACCCCAG GAACTCAGGG AATGCCAGGA 
CCTCAAGGAC ATACTGGACC TCCAGGAGAA AAGGGTCCAA CAGGAAAACC GGGTTTACCA 
GGAATGCCTG GAGCTGATGG CCCTCCTGGT CACCCAGGAA AGGAAGGCCC ATCAGGCACC 
AAAGGAAACC AGGGTCCCAG TGGTCCCCAG GGGCCTATTG GCTATCCTGG TCCTCGTGGC 
CTCAAGGGCG GTCAAGGAAT CCGGGGATTG AAGGGTCACA AAGGAGAGAA GGGAGAAGAT 
GGTTTCCCTG GAATTAAGGG AGACTTTGGG ATCAAGGGTG AGAGGGGTGA GATTGGAGTA 
CCAGGTCCTA GAGGAGAGGA CGGTCCAGAG GGGCCAAAAG GTCGTTTTGG TCCCCCTGGT 
GAAACTGGAC CACTCGGAGC AGTAGGAGAG AAGGGTAAAC TTGGTGTACC GGGACTTCCT 
GGATATCCTG GAAGACAAGG ACCAAAGGGA TCTCTTGGAT TCCCAGGATT CCCTGGATCC 
AATGGCGAGA AGGGAACAAG GGGTCTCACT GGCAAATCAG GACCAAGAGG ACAAAGAGGA 
CCAACGGGCC CCAGAGGGCA GAGGGGACCG AGGGGCGCAA CTGGCAAACT GGGGCCAAAG 
GGGACTTCGG GAAGTGATGG CCCCCCTGGT CCTCCTGGAG AAAGGGGCTT GCCTGGGCCT 
CAGGGAGCCA ATGGGTTCCC CGGACCTAAA GGGCCACCGG GGCCACCAGG GAAGGACGGG 
CTGCCTGGAC ACCCTGGGCA GAGAGGAGAA GTGGGTTTCC AAGGTAAAAT GGGTCCACCT 
GGGCCTCCTG GAGTTGTTGG ACCTCAGGGT CCAGCTGGAG AGACTGGCCC TAATGGTGAA 
CGTGGCCACC CTGGACCACC AGGTCCACCA GGAGAACAGG GACTTCCTGG TCCCTCAGGA 
AAGGAGGGAA CCAAAGGAGA CCCTGGACCC CCAGGAGGCC CTGGCAAAGA TGGGCCTCCT 
GGACTGAGGG GCTTCCCTGG TGAGAGAGGA CTGCCAGGAA CACCTGGAGG TGGAGGATTC 
AAAGGAAGCG AAGGACCTGC TGGACCTCCT GGACCGGCTG GCTCTCCTGG TGAGAGGGGG 
CAATCTGGTA CCGCTGGGCC TGTTGGACCT CCTGGAAGAC CAGGTCCTCA GGGACCTCCT 
GGACCTGCAG GAGAGAAAGG TGTTTCTGGT GAGAAAGGCC CAATTGGCCC AGCTGGTCGT 
GACGGACTAC AGGGTCCTGT GGGTCTGCCT GGCCCAGCTG GACCGCCCGG AGTACCTGGA 
GAAGATGGAG ATAAGGGGGA GGTTGGAGAA CACGGTCAGA AAGGTGCCAA GGGAGGGAAA 
GGAGAGCATG GTCCTCCAGG TCCACCTGGA CCAATGGGAC CAGTCGGCCA GCCTGGTGCT 
CCAGGTGCTG ATGGAGAGCT TGGGCCGAGG GGTCAGCAGG GACCTTTTGG AGCTAAAGGG 
GATGAAGGAA CCAGAGGATT CCCAGGAGCC CCAGGACCCA TTGGACTCCA GGGTTTGCCT 
GGGCCACCAG GTGAGAAGGG AGAAACCGGA GATGTTGGAC CTCTGGGACC TCCAGGCCCT 
CCAGGACCCC GTGGCCCTGC TGGACCCAAC GGTGCTGATG GCCCTCAAGG GCCTCCTGGT 
GGTTTGGGTA ATCCTGGACC TATCGGTGAG AAGGGAGAGC CTGGAGAGTC TGGACCCCCT 
GGAATTGGAG GGGAGCCTGG AAAGAAGGGC CCCAGAGGGG AACGTGGGGA GAAGGGGGAG 
GCTGGACAAC CAGGAACCGC TGGATCCACT GGAGGACGTG GACTACCTGG AGATGATGGA 
CCCAAAGGAA ACCCAGGTCC TGTTGGTTTC CCCGGTGATC CTGGTCCACC GGGTGAGGTT 
GGACCCAGAG GTCAAGACGG TGCCAAGGGA GAAAGAGGAG AAGATGGTGA AGCAGGAGAG 
TCTGGATCCC CTGGACCTCC CGGTGAGAAC GGACCCCCCG GCCCCCTAGG AAAGAGGGGT 
CCTGCTGGAA CAAGAGGACC AGAGGGACGC CAGGGAGAGA AGGGAACAAA GGGGGATCCA 
GGTGCTGTTG GCCCCCCAGG CAAAACAGGC CCTGTGGGTC CACAGGGAGT GCCAGGAAAA 
CCTGGAACAG AAGGCCTTAG AGGGCTTCCA GGATCAGTGG GTGAGCAAGG ATCTCCAGGA 
CCTGCAGGAC AGAAAGGACC ACCTGGTCCT ATTGGACCTC CCGGTTTACC TGGTCTGCGT 
GGAGATTCTG GCGGCAAAGG AGAAAAGGGA CACCCGGGTC TTATCGGTCT CATTGGACCC 
CCAGGAGAAC AGGGTGAGAA GGGAGACAGA GGCATGCCTG GGCCCCATGG ATCCAATGGA 
CCAAAAGGAG AGACTGGAAT GCCTGGAGGC ACAGGACCTA TCGGCCCCTC TGGTCCTCCT 
GGTCTGCCTG GTCCTCAAGG TATTAAAGGA GCCAAAGGTT CTTCTGGTGG AGCTGGTCCA 
AAAGGAGAAA AAGGAGTACA AGGACCCCCT GGACTTCCTG GCCCACCAGG GGAGGTTATT 
CAGCCACTAC CCATCCAAAG GAGTCCCAAG TCCAAGCGGT CCATTGATGC CAGCCAGCTA 
CTTCCTGAGA ATGACATGCC TGCATCCGAT GCCACCGGGA CAGAATTCCT GATGGGTAGT 
GAAGGCATGG AGGAGATCTT TGGCTCCCTC AACTCGCTTC GGCAGGAGAT TGAGACCATG 
CGCTTCCCTC TGGGCACCCA GGACAGCCCC GGCCGCACCT GCCAAGACCT GCACCTCAGT 
CAGCCAGAAC TCAAAGATGG AGAATACTGG ATTGACCCCA ACCAGGGCTG CTCCAGAGAC 
GCCTTCAAGG TCTTCTGTAA CTTTACTAAG GGGGCAGAGA CGTGCCTCTA CCCCAGCCAG 
AGCATCAGCA CGGTGAAGAT GAATTCCTGG AACAAAGAGA CTCCAGGTTC CTGGTACAGT 
CAGTTCTCCA CTGGCAGTAA GTTTTCATAC GTGGACTCCA ACGGCGAACC TGTGGGTGTG 
GTTCAGTTGG GTTTCCTTCG TCTCTTGAGC GTGCAGGCCC ACCAGACTCT CACATACCAC 
TGCCACCGCT CGGTAGCGTG GGCTGACCGC AACGCCAAGA ACACATACAG CCGAGCTCTG 
CACTTCAGGG GTGCCAACGA CGAGGATCTA AGCTATGAGA CCAATCCATA CGTCAAGGCT 
TTGGTTGATG GCTGCTCTTA TCGTAAGGGC TTTGACCGGA CAGTCCTGGA GATCAACACA 
CCACAGGTGG AACATCTTCC TCTGCTGGAT ATCAAGGTGT CAGACTTTGG GGAGAGCAAC 
CAGCAGTTTG GCTTTGAAGT GGGACCTGTC TGTTTCCAAG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101066527 ( XM_011617367.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011615669.2 Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X3, mRNA.

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CloneID	OTo45739
Clone ID Related Accession (Same CDS sequence)	XM_011617367.2
Accession Version	XM_011617367.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	collagen alpha-1(V) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042296.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011617367.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGAC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATAAG 
AAGCAGCTCA ATGGAAAAGG AGCCCCCACT AAATCCACTG CTGTTAAAGC AAAACCTACC 
CCAGCAAAGC CAGCCAAGTC TGGATCATAT AAGCTTGTAG TCAAGTCCCC TATGCCCATC 
AAAGCAGCAA AGTCTACAGC CAAGCCATTT AAAGGCAGGC CCACTGCAGT AGAGATCCTT 
CTCTCTAAGA TAAACCCAAT AACACCATCT AAATCCACAA TTTCCTTGAT TAAAAATGGC 
AACGGTAAAT CAGCTGCAGC AAAGACAAAT GGATTGGCCA AAGCCAACAA CAATGGAAAT 
GGAAGAGCAA CAGGCGCTAA AGCAAATGGC AATGGCAAAG CTGATTCTAA GCCCATAGCT 
CAGGCCAGAG TGAGCGGAAA TGGCAAAGTT GTAGTGGAGA AAAAAGTCAT CATAAATGCC 
AAATCTTCAG CAGAAAAGAA ATCCAGCGGA AATGGAAAAG TCACCATTGT AACCAAGAGT 
AATGGTGCAG CAGAGAAGAA AACCAGTGGT AGTGGCAAAG CTACCATTGA GGTTAAAGAA 
ATTAAAGTCA AAGTTCATGG AAAACCTGAA AATAAAGTTG CAGGTGAGGC CAAGGCTAAC 
GGCAATGGTA AAATTATCAC TGTGGTAGAA AAGAAATTCA CCAATGGTGC TGCGAATCGG 
GCAAAGATTG AAACCACTAC AAGAGCAAAG ACGGAGAAAG TTATCAAAGT TGAAAAGATA 
GAAAAGACTA CGCTCAGTAC AGCTAAATCA GCCACAAAAC TAGATGCTTC TAAAGCAGCC 
AAACCCACAA AAGCCACAAA ATCTGATGCT ACAACATTAA CAAAGGCCAC CAAAGCAGCG 
GCAGCAGCAG GAAAGACCCA AGCAAGGGCA GAGGTCAAAG AAGTCACCCA AGTTAAAACT 
GTTGTCACCA AAGTCCCTCT GGTCAAAACC ACTGTGAGCA AACCAGCGAA GAATGTGGTG 
GAGAGAGTTA CGATGCCCAG GAAAGTAGCC TCGACGTCCA TGCCTGTGAG GCCCACTCCA 
CCCAGACCTA CGAAACCCAA GATGGCGGTG GACATTAATG TCAAATCCCA GCACAAACCT 
TTCCCACCAT TTGGCAAGAC TGCGTTGAGT GGAACTACGA AGAAAGTTAC CAACGGTTAT 
CAGCAGGCTG GGGTGAGAGG CCAGAAAGGA GAGAAAGGAG AACCTGCTGT TTTGGAGCCT 
GGGATGCTGA TTGAAGGACC ACCAGGACCT GAGGGACCTG CTGGCCTACC TGGTCCTTCT 
GGCCCTTCTG GACCTCCTGG CTCTGTGGGT GACCCAGGAG AGAGGGGCCC TCCTGGTAAG 
GCTGGTCTTC CTGGAGCTGA TGGAGTCCCT GGACCTCCTG GGACCTCTGT TATGCTGCCT 
TTCCGTTTTG GTCAGAGTGG AGGAGATAAA GGTCCTGCTG TTTCTGCTCA GGAAGCTCAG 
GCAGCAGCCA TCTTGTCTCA GGCCAGGATG GCTCTTAAAG GACCTCCTGG TCCAATGGGA 
TTCACTGGGC GCCCCGGACC CTTGGGAAAT CCTGGAAGTC CTGGTCTGAA AGGAGAGAGT 
GGAGACCCTG GACCCCAGGG CCCGAGGGGA TCTCTAGGCT TGATGGGACC TCCAGGCAAA 
TCAGGAAGAA GAGGCCGCGC TGGAGCCGAC GGTGCCAGAG GAATGCCTGG AGAGTCTGGT 
TCTAAGGGTG ATCGTGGTTT TGATGGTCTT CCTGGTTTAC CTGGAGACAA AGGACACAGG 
GGTGACTCGG GACCAATGGG ACCTCCAGGA TCTCAGGGAG AGGATGGAGA AAGGGGAGAC 
GACGGAGACG TTGGACCCCG GGGTCTTCCA GGTGAACCAG GACCTCGTGG TTTGCTTGGA 
CCTAAAGGTC CTCCTGGAAT CCCAGGCCCT CCTGGTGTAC GAGGAAGTGA CGGACCCCAC 
GGTCCAAAAG GAAACTTGGG TCCTCAAGGT GAGCCAGGAC CCCCTGGTCA GCAGGGAACC 
CCAGGAACTC AGGGAATGCC AGGACCTCAA GGACATACTG GACCTCCAGG AGAAAAGGGT 
CCAACAGGAA AACCGGGTTT ACCAGGAATG CCTGGAGCTG ATGGCCCTCC TGGTCACCCA 
GGAAAGGAAG GCCCATCAGG CACCAAAGGA AACCAGGGTC CCAGTGGTCC CCAGGGGCCT 
ATTGGCTATC CTGGTCCTCG TGGCCTCAAG GGCGGTCAAG GAATCCGGGG ATTGAAGGGT 
CACAAAGGAG AGAAGGGAGA AGATGGTTTC CCTGGAATTA AGGGAGACTT TGGGATCAAG 
GGTGAGAGGG GTGAGATTGG AGTACCAGGT CCTAGAGGAG AGGACGGTCC AGAGGGGCCA 
AAAGGTCGTT TTGGTCCCCC TGGTGAAACT GGACCACTCG GAGCAGTAGG AGAGAAGGGT 
AAACTTGGTG TACCGGGACT TCCTGGATAT CCTGGAAGAC AAGGACCAAA GGGATCTCTT 
GGATTCCCAG GATTCCCTGG ATCCAATGGC GAGAAGGGAA CAAGGGGTCT CACTGGCAAA 
TCAGGACCAA GAGGACAAAG AGGACCAACG GGCCCCAGAG GGCAGAGGGG ACCGAGGGGC 
GCAACTGGCA AACTGGGGCC AAAGGGGACT TCGGGAAGTG ATGGCCCCCC TGGTCCTCCT 
GGAGAAAGGG GCTTGCCTGG GCCTCAGGGA GCCAATGGGT TCCCCGGACC TAAAGGGCCA 
CCGGGGCCAC CAGGGAAGGA CGGGCTGCCT GGACACCCTG GGCAGAGAGG AGAAGTGGGT 
TTCCAAGGTA AAATGGGTCC ACCTGGGCCT CCTGGAGTTG TTGGACCTCA GGGTCCAGCT 
GGAGAGACTG GCCCTAATGG TGAACGTGGC CACCCTGGAC CACCAGGTCC ACCAGGAGAA 
CAGGGACTTC CTGGTCCCTC AGGAAAGGAG GGAACCAAAG GAGACCCTGG ACCCCCAGGA 
GGCCCTGGCA AAGATGGGCC TCCTGGACTG AGGGGCTTCC CTGGTGAGAG AGGACTGCCA 
GGAACACCTG GAGGTGGAGG ATTCAAAGGA AGCGAAGGAC CTGCTGGACC TCCTGGACCG 
GCTGGCTCTC CTGGTGAGAG GGGGCAATCT GGTACCGCTG GGCCTGTTGG ACCTCCTGGA 
AGACCAGGTC CTCAGGGACC TCCTGGACCT GCAGGAGAGA AAGGTGTTTC TGGTGAGAAA 
GGCCCAATTG GCCCAGCTGG TCGTGACGGA CTACAGGGTC CTGTGGGTCT GCCTGGCCCA 
GCTGGACCGC CCGGAGTACC TGGAGAAGAT GGAGATAAGG GGGAGGTTGG AGAACACGGT 
CAGAAAGGTG CCAAGGGAGG GAAAGGAGAG CATGGTCCTC CAGGTCCACC TGGACCAATG 
GGACCAGTCG GCCAGCCTGG TGCTCCAGGT GCTGATGGAG AGCTTGGGCC GAGGGGTCAG 
CAGGGACCTT TTGGAGCTAA AGGGGATGAA GGAACCAGAG GATTCCCAGG AGCCCCAGGA 
CCCATTGGAC TCCAGGGTTT GCCTGGGCCA CCAGGTGAGA AGGGAGAAAC CGGAGATGTT 
GGACCTCTGG GACCTCCAGG CCCTCCAGGA CCCCGTGGCC CTGCTGGACC CAACGGTGCT 
GATGGCCCTC AAGGGCCTCC TGGTGGTTTG GGTAATCCTG GACCTATCGG TGAGAAGGGA 
GAGCCTGGAG AGTCTGGACC CCCTGGAATT GGAGGGGAGC CTGGAAAGAA GGGCCCCAGA 
GGGGAACGTG GGGAGAAGGG GGAGGCTGGA CAACCAGGAA CCGCTGGATC CACTGGAGGA 
CGTGGACTAC CTGGAGATGA TGGACCCAAA GGAAACCCAG GTCCTGTTGG TTTCCCCGGT 
GATCCTGGTC CACCGGGTGA GGTTGGACCC AGAGGTCAAG ACGGTGCCAA GGGAGAAAGA 
GGAGAAGATG GTGAAGCAGG AGAGTCTGGA TCCCCTGGAC CTCCCGGTGA GAACGGACCC 
CCCGGCCCCC TAGGAAAGAG GGGTCCTGCT GGAACAAGAG GACCAGAGGG ACGCCAGGGA 
GAGAAGGGAA CAAAGGGGGA TCCAGGTGCT GTTGGCCCCC CAGGCAAAAC AGGCCCTGTG 
GGTCCACAGG GAGTGCCAGG AAAACCTGGA ACAGAAGGCC TTAGAGGGCT TCCAGGATCA 
GTGGGTGAGC AAGGATCTCC AGGACCTGCA GGACAGAAAG GACCACCTGG TCCTATTGGA 
CCTCCCGGTT TACCTGGTCT GCGTGGAGAT TCTGGCGGCA AAGGAGAAAA GGGACACCCG 
GGTCTTATCG GTCTCATTGG ACCCCCAGGA GAACAGGGTG AGAAGGGAGA CAGAGGCATG 
CCTGGGCCCC ATGGATCCAA TGGACCAAAA GGAGAGACTG GAATGCCTGG AGGCACAGGA 
CCTATCGGCC CCTCTGGTCC TCCTGGTCTG CCTGGTCCTC AAGGTATTAA AGGAGCCAAA 
GGTTCTTCTG GTGGAGCTGG TCCAAAAGGA GAAAAAGGAG TACAAGGACC CCCTGGACTT 
CCTGGCCCAC CAGGGGAGGT TATTCAGCCA CTACCCATCC AAAGGAGTCC CAAGTCCAAG 
CGGTCCATTG ATGCCAGCCA GCTACTTCCT GAGAATGACA TGCCTGCATC CGATGCCACC 
GGGACAGAAT TCCTGATGGG TAGTGAAGGC ATGGAGGAGA TCTTTGGCTC CCTCAACTCG 
CTTCGGCAGG AGATTGAGAC CATGCGCTTC CCTCTGGGCA CCCAGGACAG CCCCGGCCGC 
ACCTGCCAAG ACCTGCACCT CAGTCAGCCA GAACTCAAAG ATGGAGAATA CTGGATTGAC 
CCCAACCAGG GCTGCTCCAG AGACGCCTTC AAGGTCTTCT GTAACTTTAC TAAGGGGGCA 
GAGACGTGCC TCTACCCCAG CCAGAGCATC AGCACGGTGA AGATGAATTC CTGGAACAAA 
GAGACTCCAG GTTCCTGGTA CAGTCAGTTC TCCACTGGCA GTAAGTTTTC ATACGTGGAC 
TCCAACGGCG AACCTGTGGG TGTGGTTCAG TTGGGTTTCC TTCGTCTCTT GAGCGTGCAG 
GCCCACCAGA CTCTCACATA CCACTGCCAC CGCTCGGTAG CGTGGGCTGA CCGCAACGCC 
AAGAACACAT ACAGCCGAGC TCTGCACTTC AGGGGTGCCA ACGACGAGGA TCTAAGCTAT 
GAGACCAATC CATACGTCAA GGCTTTGGTT GATGGCTGCT CTTATCGTAA GGGCTTTGAC 
CGGACAGTCC TGGAGATCAA CACACCACAG GTGGAACATC TTCCTCTGCT GGATATCAAG 
GTGTCAGACT TTGGGGAGAG CAACCAGCAG TTTGGCTTTG AAGTGGGACC TGTCTGTTTC 
CAAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615669.2
CDS	408..6536
Translation	MDTGDCPCRKKRRPWRMDFSSFALVTVMLIVCQTALVRADPVDVLRALQVPSLPEGVKKVPGFCTSRRSSSPDHAYRITKKAQISAPTKQLFSGRFPENFSIMALVKAQAGLQAFLLSIYSEQGIQQLGIEMGRSPVFLYEDQNGKPAPEDYPLFRGVNLADGKWHRIALSVSKKNVTLLLDCKKKMTRPLPRSNSAEVDTNGITVFGARLLDEEVFQGDIQQLLIASNPQAAYDFCEHYSPDCDSPLPKTQAQDPNTYKKQLNGKGAPTKSTAVKAKPTPAKPAKSGSYKLVVKSPMPIKAAKSTAKPFKGRPTAVEILLSKINPITPSKSTISLIKNGNGKSAAAKTNGLAKANNNGNGRATGAKANGNGKADSKPIAQARVSGNGKVVVEKKVIINAKSSAEKKSSGNGKVTIVTKSNGAAEKKTSGSGKATIEVKEIKVKVHGKPENKVAGEAKANGNGKIITVVEKKFTNGAANRAKIETTTRAKTEKVIKVEKIEKTTLSTAKSATKLDASKAAKPTKATKSDATTLTKATKAAAAAGKTQARAEVKEVTQVKTVVTKVPLVKTTVSKPAKNVVERVTMPRKVASTSMPVRPTPPRPTKPKMAVDINVKSQHKPFPPFGKTALSGTTKKVTNGYQQAGVRGQKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPSGPPGSVGDPGERGPPGKAGLPGADGVPGPPGTSVMLPFRFGQSGGDKGPAVSAQEAQAAAILSQARMALKGPPGPMGFTGRPGPLGNPGSPGLKGESGDPGPQGPRGSLGLMGPPGKSGRRGRAGADGARGMPGESGSKGDRGFDGLPGLPGDKGHRGDSGPMGPPGSQGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGIPGPPGVRGSDGPHGPKGNLGPQGEPGPPGQQGTPGTQGMPGPQGHTGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGPIGYPGPRGLKGGQGIRGLKGHKGEKGEDGFPGIKGDFGIKGERGEIGVPGPRGEDGPEGPKGRFGPPGETGPLGAVGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGSNGEKGTRGLTGKSGPRGQRGPTGPRGQRGPRGATGKLGPKGTSGSDGPPGPPGERGLPGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKMGPPGPPGVVGPQGPAGETGPNGERGHPGPPGPPGEQGLPGPSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGGGGFKGSEGPAGPPGPAGSPGERGQSGTAGPVGPPGRPGPQGPPGPAGEKGVSGEKGPIGPAGRDGLQGPVGLPGPAGPPGVPGEDGDKGEVGEHGQKGAKGGKGEHGPPGPPGPMGPVGQPGAPGADGELGPRGQQGPFGAKGDEGTRGFPGAPGPIGLQGLPGPPGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGIGGEPGKKGPRGERGEKGEAGQPGTAGSTGGRGLPGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGERGEDGEAGESGSPGPPGENGPPGPLGKRGPAGTRGPEGRQGEKGTKGDPGAVGPPGKTGPVGPQGVPGKPGTEGLRGLPGSVGEQGSPGPAGQKGPPGPIGPPGLPGLRGDSGGKGEKGHPGLIGLIGPPGEQGEKGDRGMPGPHGSNGPKGETGMPGGTGPIGPSGPPGLPGPQGIKGAKGSSGGAGPKGEKGVQGPPGLPGPPGEVIQPLPIQRSPKSKRSIDASQLLPENDMPASDATGTEFLMGSEGMEEIFGSLNSLRQEIETMRFPLGTQDSPGRTCQDLHLSQPELKDGEYWIDPNQGCSRDAFKVFCNFTKGAETCLYPSQSISTVKMNSWNKETPGSWYSQFSTGSKFSYVDSNGEPVGVVQLGFLRLLSVQAHQTLTYHCHRSVAWADRNAKNTYSRALHFRGANDEDLSYETNPYVKALVDGCSYRKGFDRTVLEINTPQVEHLPLLDIKVSDFGESNQQFGFEVGPVCFQG

Target ORF information:

RefSeq Version	XM_011617367.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes collagen alpha-1(XI) chain (LOC101066527), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617367.2

ORF Insert Sequence:

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGAC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATAAG 
AAGCAGCTCA ATGGAAAAGG AGCCCCCACT AAATCCACTG CTGTTAAAGC AAAACCTACC 
CCAGCAAAGC CAGCCAAGTC TGGATCATAT AAGCTTGTAG TCAAGTCCCC TATGCCCATC 
AAAGCAGCAA AGTCTACAGC CAAGCCATTT AAAGGCAGGC CCACTGCAGT AGAGATCCTT 
CTCTCTAAGA TAAACCCAAT AACACCATCT AAATCCACAA TTTCCTTGAT TAAAAATGGC 
AACGGTAAAT CAGCTGCAGC AAAGACAAAT GGATTGGCCA AAGCCAACAA CAATGGAAAT 
GGAAGAGCAA CAGGCGCTAA AGCAAATGGC AATGGCAAAG CTGATTCTAA GCCCATAGCT 
CAGGCCAGAG TGAGCGGAAA TGGCAAAGTT GTAGTGGAGA AAAAAGTCAT CATAAATGCC 
AAATCTTCAG CAGAAAAGAA ATCCAGCGGA AATGGAAAAG TCACCATTGT AACCAAGAGT 
AATGGTGCAG CAGAGAAGAA AACCAGTGGT AGTGGCAAAG CTACCATTGA GGTTAAAGAA 
ATTAAAGTCA AAGTTCATGG AAAACCTGAA AATAAAGTTG CAGGTGAGGC CAAGGCTAAC 
GGCAATGGTA AAATTATCAC TGTGGTAGAA AAGAAATTCA CCAATGGTGC TGCGAATCGG 
GCAAAGATTG AAACCACTAC AAGAGCAAAG ACGGAGAAAG TTATCAAAGT TGAAAAGATA 
GAAAAGACTA CGCTCAGTAC AGCTAAATCA GCCACAAAAC TAGATGCTTC TAAAGCAGCC 
AAACCCACAA AAGCCACAAA ATCTGATGCT ACAACATTAA CAAAGGCCAC CAAAGCAGCG 
GCAGCAGCAG GAAAGACCCA AGCAAGGGCA GAGGTCAAAG AAGTCACCCA AGTTAAAACT 
GTTGTCACCA AAGTCCCTCT GGTCAAAACC ACTGTGAGCA AACCAGCGAA GAATGTGGTG 
GAGAGAGTTA CGATGCCCAG GAAAGTAGCC TCGACGTCCA TGCCTGTGAG GCCCACTCCA 
CCCAGACCTA CGAAACCCAA GATGGCGGTG GACATTAATG TCAAATCCCA GCACAAACCT 
TTCCCACCAT TTGGCAAGAC TGCGTTGAGT GGAACTACGA AGAAAGTTAC CAACGGTTAT 
CAGCAGGCTG GGGTGAGAGG CCAGAAAGGA GAGAAAGGAG AACCTGCTGT TTTGGAGCCT 
GGGATGCTGA TTGAAGGACC ACCAGGACCT GAGGGACCTG CTGGCCTACC TGGTCCTTCT 
GGCCCTTCTG GACCTCCTGG CTCTGTGGGT GACCCAGGAG AGAGGGGCCC TCCTGGTAAG 
GCTGGTCTTC CTGGAGCTGA TGGAGTCCCT GGACCTCCTG GGACCTCTGT TATGCTGCCT 
TTCCGTTTTG GTCAGAGTGG AGGAGATAAA GGTCCTGCTG TTTCTGCTCA GGAAGCTCAG 
GCAGCAGCCA TCTTGTCTCA GGCCAGGATG GCTCTTAAAG GACCTCCTGG TCCAATGGGA 
TTCACTGGGC GCCCCGGACC CTTGGGAAAT CCTGGAAGTC CTGGTCTGAA AGGAGAGAGT 
GGAGACCCTG GACCCCAGGG CCCGAGGGGA TCTCTAGGCT TGATGGGACC TCCAGGCAAA 
TCAGGAAGAA GAGGCCGCGC TGGAGCCGAC GGTGCCAGAG GAATGCCTGG AGAGTCTGGT 
TCTAAGGGTG ATCGTGGTTT TGATGGTCTT CCTGGTTTAC CTGGAGACAA AGGACACAGG 
GGTGACTCGG GACCAATGGG ACCTCCAGGA TCTCAGGGAG AGGATGGAGA AAGGGGAGAC 
GACGGAGACG TTGGACCCCG GGGTCTTCCA GGTGAACCAG GACCTCGTGG TTTGCTTGGA 
CCTAAAGGTC CTCCTGGAAT CCCAGGCCCT CCTGGTGTAC GAGGAAGTGA CGGACCCCAC 
GGTCCAAAAG GAAACTTGGG TCCTCAAGGT GAGCCAGGAC CCCCTGGTCA GCAGGGAACC 
CCAGGAACTC AGGGAATGCC AGGACCTCAA GGACATACTG GACCTCCAGG AGAAAAGGGT 
CCAACAGGAA AACCGGGTTT ACCAGGAATG CCTGGAGCTG ATGGCCCTCC TGGTCACCCA 
GGAAAGGAAG GCCCATCAGG CACCAAAGGA AACCAGGGTC CCAGTGGTCC CCAGGGGCCT 
ATTGGCTATC CTGGTCCTCG TGGCCTCAAG GGCGGTCAAG GAATCCGGGG ATTGAAGGGT 
CACAAAGGAG AGAAGGGAGA AGATGGTTTC CCTGGAATTA AGGGAGACTT TGGGATCAAG 
GGTGAGAGGG GTGAGATTGG AGTACCAGGT CCTAGAGGAG AGGACGGTCC AGAGGGGCCA 
AAAGGTCGTT TTGGTCCCCC TGGTGAAACT GGACCACTCG GAGCAGTAGG AGAGAAGGGT 
AAACTTGGTG TACCGGGACT TCCTGGATAT CCTGGAAGAC AAGGACCAAA GGGATCTCTT 
GGATTCCCAG GATTCCCTGG ATCCAATGGC GAGAAGGGAA CAAGGGGTCT CACTGGCAAA 
TCAGGACCAA GAGGACAAAG AGGACCAACG GGCCCCAGAG GGCAGAGGGG ACCGAGGGGC 
GCAACTGGCA AACTGGGGCC AAAGGGGACT TCGGGAAGTG ATGGCCCCCC TGGTCCTCCT 
GGAGAAAGGG GCTTGCCTGG GCCTCAGGGA GCCAATGGGT TCCCCGGACC TAAAGGGCCA 
CCGGGGCCAC CAGGGAAGGA CGGGCTGCCT GGACACCCTG GGCAGAGAGG AGAAGTGGGT 
TTCCAAGGTA AAATGGGTCC ACCTGGGCCT CCTGGAGTTG TTGGACCTCA GGGTCCAGCT 
GGAGAGACTG GCCCTAATGG TGAACGTGGC CACCCTGGAC CACCAGGTCC ACCAGGAGAA 
CAGGGACTTC CTGGTCCCTC AGGAAAGGAG GGAACCAAAG GAGACCCTGG ACCCCCAGGA 
GGCCCTGGCA AAGATGGGCC TCCTGGACTG AGGGGCTTCC CTGGTGAGAG AGGACTGCCA 
GGAACACCTG GAGGTGGAGG ATTCAAAGGA AGCGAAGGAC CTGCTGGACC TCCTGGACCG 
GCTGGCTCTC CTGGTGAGAG GGGGCAATCT GGTACCGCTG GGCCTGTTGG ACCTCCTGGA 
AGACCAGGTC CTCAGGGACC TCCTGGACCT GCAGGAGAGA AAGGTGTTTC TGGTGAGAAA 
GGCCCAATTG GCCCAGCTGG TCGTGACGGA CTACAGGGTC CTGTGGGTCT GCCTGGCCCA 
GCTGGACCGC CCGGAGTACC TGGAGAAGAT GGAGATAAGG GGGAGGTTGG AGAACACGGT 
CAGAAAGGTG CCAAGGGAGG GAAAGGAGAG CATGGTCCTC CAGGTCCACC TGGACCAATG 
GGACCAGTCG GCCAGCCTGG TGCTCCAGGT GCTGATGGAG AGCTTGGGCC GAGGGGTCAG 
CAGGGACCTT TTGGAGCTAA AGGGGATGAA GGAACCAGAG GATTCCCAGG AGCCCCAGGA 
CCCATTGGAC TCCAGGGTTT GCCTGGGCCA CCAGGTGAGA AGGGAGAAAC CGGAGATGTT 
GGACCTCTGG GACCTCCAGG CCCTCCAGGA CCCCGTGGCC CTGCTGGACC CAACGGTGCT 
GATGGCCCTC AAGGGCCTCC TGGTGGTTTG GGTAATCCTG GACCTATCGG TGAGAAGGGA 
GAGCCTGGAG AGTCTGGACC CCCTGGAATT GGAGGGGAGC CTGGAAAGAA GGGCCCCAGA 
GGGGAACGTG GGGAGAAGGG GGAGGCTGGA CAACCAGGAA CCGCTGGATC CACTGGAGGA 
CGTGGACTAC CTGGAGATGA TGGACCCAAA GGAAACCCAG GTCCTGTTGG TTTCCCCGGT 
GATCCTGGTC CACCGGGTGA GGTTGGACCC AGAGGTCAAG ACGGTGCCAA GGGAGAAAGA 
GGAGAAGATG GTGAAGCAGG AGAGTCTGGA TCCCCTGGAC CTCCCGGTGA GAACGGACCC 
CCCGGCCCCC TAGGAAAGAG GGGTCCTGCT GGAACAAGAG GACCAGAGGG ACGCCAGGGA 
GAGAAGGGAA CAAAGGGGGA TCCAGGTGCT GTTGGCCCCC CAGGCAAAAC AGGCCCTGTG 
GGTCCACAGG GAGTGCCAGG AAAACCTGGA ACAGAAGGCC TTAGAGGGCT TCCAGGATCA 
GTGGGTGAGC AAGGATCTCC AGGACCTGCA GGACAGAAAG GACCACCTGG TCCTATTGGA 
CCTCCCGGTT TACCTGGTCT GCGTGGAGAT TCTGGCGGCA AAGGAGAAAA GGGACACCCG 
GGTCTTATCG GTCTCATTGG ACCCCCAGGA GAACAGGGTG AGAAGGGAGA CAGAGGCATG 
CCTGGGCCCC ATGGATCCAA TGGACCAAAA GGAGAGACTG GAATGCCTGG AGGCACAGGA 
CCTATCGGCC CCTCTGGTCC TCCTGGTCTG CCTGGTCCTC AAGGTATTAA AGGAGCCAAA 
GGTTCTTCTG GTGGAGCTGG TCCAAAAGGA GAAAAAGGAG TACAAGGACC CCCTGGACTT 
CCTGGCCCAC CAGGGGAGGT TATTCAGCCA CTACCCATCC AAAGGAGTCC CAAGTCCAAG 
CGGTCCATTG ATGCCAGCCA GCTACTTCCT GAGAATGACA TGCCTGCATC CGATGCCACC 
GGGACAGAAT TCCTGATGGG TAGTGAAGGC ATGGAGGAGA TCTTTGGCTC CCTCAACTCG 
CTTCGGCAGG AGATTGAGAC CATGCGCTTC CCTCTGGGCA CCCAGGACAG CCCCGGCCGC 
ACCTGCCAAG ACCTGCACCT CAGTCAGCCA GAACTCAAAG ATGGAGAATA CTGGATTGAC 
CCCAACCAGG GCTGCTCCAG AGACGCCTTC AAGGTCTTCT GTAACTTTAC TAAGGGGGCA 
GAGACGTGCC TCTACCCCAG CCAGAGCATC AGCACGGTGA AGATGAATTC CTGGAACAAA 
GAGACTCCAG GTTCCTGGTA CAGTCAGTTC TCCACTGGCA GTAAGTTTTC ATACGTGGAC 
TCCAACGGCG AACCTGTGGG TGTGGTTCAG TTGGGTTTCC TTCGTCTCTT GAGCGTGCAG 
GCCCACCAGA CTCTCACATA CCACTGCCAC CGCTCGGTAG CGTGGGCTGA CCGCAACGCC 
AAGAACACAT ACAGCCGAGC TCTGCACTTC AGGGGTGCCA ACGACGAGGA TCTAAGCTAT 
GAGACCAATC CATACGTCAA GGCTTTGGTT GATGGCTGCT CTTATCGTAA GGGCTTTGAC 
CGGACAGTCC TGGAGATCAA CACACCACAG GTGGAACATC TTCCTCTGCT GGATATCAAG 
GTGTCAGACT TTGGGGAGAG CAACCAGCAG TTTGGCTTTG AAGTGGGACC TGTCTGTTTC 
CAAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101066527 ( XM_011617365.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011615667.1 Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X1, mRNA.

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CloneID	OTo22616
Clone ID Related Accession (Same CDS sequence)	XM_011617365.1
Accession Version	XM_011617365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	collagen alpha-2(XI) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071261.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGGC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATAAG 
AAGCAGCTCA ATGGAAAAGG AGCCCCCACT AAATCCACTG CTGTTAAAGC AAAACCTACC 
CCAGCAAAGC CAGCCAAGTC TGGATCATAT AAGCTTGTAG TCAAGTCCCC TATGCCCATC 
AAAGCAGCAA AGTCTACAGC CAAGCCATTT AAAGGCAGGC CCACTGCAGT AGAGATCCTT 
CTCTCTAAGA TAAACCCAAT AACACCATCT AAATCCACAA TTTCCTTGAT TAAAAATGGC 
AACGGTAAAT CAGCTGCAGC AAAGACAAAT GGATTGGCCA AAGCCAACAA CAATGGAAAT 
GGAAGAGCAA CAGGCGCTAA AGTAAATGGC AATGGCAAAG CTGATTCTAA GCCCACAGCT 
CAGGCCAGAG TGAGCGGAAA TGGCAAAGTT GTAGTGGAGA AAAAAGTCAT CATAAATGCC 
AAATCTTCAG CAGAAAAGAA ATCCAGCGGA AATGGAAAAG TCACCATTGT AACCAAGAGT 
AATGGTGCAG CAGAGAAGAA AACCAGTGGT AGTGGCAAAG CTACCATTGA GGTTAAAGAA 
ATTAAAGTCA AAGTTCATGG AAAACCTGAA AATAAAGTTG CAGGTGAGGC CAAGGCTAAC 
GGCAATGGTA AAATTATCAC TGTGGTAGAA AAGAAATTCA CCAATGGTGC TGCGAATCGG 
GCAAAGATTG AAACCACTAC AAGAGCAAAG ACGGAGAAAG TTATCAAAGT TGAAAAGATA 
GAAAAGACTA CGCTCAGTAC AGCTAAATCA GCCACAAAAC TAGATGCTTC TAAAGCAGCC 
AAACCCACAA AAGCCACAAA ATCTGATGCT ACAACATTAA CAAAGGCCAC CAAAGCAGCG 
GCAGCAGCAG GAAAGACCCA AGCAAGGGCA GAGGTCAAAG AAGTCACCCA AGTTAAAACT 
GTTGTCACCA AAGTCCCTCT GGTCAAAACC ACTGTGAGCA AACCAGCGAA GAATGTGGTG 
GAGAGAGTTA CGATGCCCAG GAAAGTAGCC TCGACGTCCA TGCCCGTGAG GCCCACTCCA 
CCCAGACCTA CGAAACCCAA GATGGCGGTG GACATTAATG TCAAATCCCA GCACAAACCT 
TTCCCACCAT TTGGCAAGAC TGCGTTGAGT GGAACTACGA AGAAAGTTAC CAACGGTTAT 
CAGCAGGATG TAGACACTGA ATATTCCGAG GCTGGCCACA CCCCTACAGA GAGAGATTAT 
TACTATGAGG AGACGGTACC ACTGCCAGAG GGTGAGGTGG TTGGGCAAGA GGAACTCGCC 
GTACAGCCCC AGGTGGAGCC TGTGTTGGAG GGAGAAGAGG TGCATTCGAC CAAGGCTGTC 
GGTGAGGGAG AGGAGGCCTT CACAGAGGAG TATGTGACTG GAGATGTGAC CCTTAAGGAA 
TACGACTATT CCTACAGGGA CTACAATGAG CCATTAGTGG AGACTGGAGA AGTCGATGCC 
AACATGGGCC CCGCCCTCTC TGCTGTGACA GACGAGGGAG GCGCTGGGGT GAGAGGCCAG 
AAAGGAGAGA AAGGAGAACC TGCTGTTTTG GAGCCTGGGA TGCTGATTGA AGGACCACCA 
GGACCTGAGG GACCTGCTGG CCTACCTGGT CCTTCTGGCC CTTCTGGACC TCCTGGCTCT 
GTGGGTGACC CAGGAGAGAG GGGCCCTCCT GGTAAGGCTG GTCTTCCTGG AGCTGATGGA 
GTCCCTGGAC CTCCTGGGAC CTCTGTTATG CTGCCTTTCC GTTTTGGTCA GAGTGGAGGA 
GATAAAGGTC CTGCTGTTTC TGCTCAGGAA GCTCAGGCAG CAGCCATCTT GTCTCAGGCC 
AGGATGGCTC TTAAAGGACC TCCTGGTCCA ATGGGATTCA CTGGGCGTCC CGGACCCTTG 
GGAAATCCTG GAAGTCCTGG TCTGAAAGGA GAGAGTGGAG ACCCTGGACC CCAGGGCCCG 
AGGGGATCTC TAGGCTTGAT GGGACCTCCA GGCAAATCAG GAAGAAGAGG TCGCGCTGGA 
GCCGACGGTG CCAGAGGAAT GCCTGGAGAG TCTGGTTCTA AGGGTGATCG TGGTTTTGAT 
GGTCTTCCTG GTTTACCTGG AGACAAAGGA CACAGGGGTG ACTCGGGACC AATGGGACCT 
CCAGGATCTC AGGGAGAGGA TGGAGAAAGG GGAGACGACG GAGACGTTGG ACCCCGGGGT 
CTTCCAGGTG AACCAGGACC TCGTGGTTTG CTTGGACCTA AAGGTCCTCC TGGAATCCCA 
GGCCCTCCTG GTGTACGAGG AAGTGACGGA CCCCACGGTC CAAAAGGAAA CTTGGGTCCT 
CAAGGTGAGC CAGGACCCCC TGGTCAGCAG GGAACCCCAG GAACTCAGGG AATGCCAGGA 
CCTCAAGGAC ATACTGGACC TCCAGGAGAA AAGGGTCCAA CAGGAAAACC GGGTTTACCA 
GGAATGCCTG GAGCTGATGG CCCTCCTGGT CACCCAGGAA AGGAAGGCCC ATCAGGCACC 
AAAGGAAACC AGGGTCCCAG TGGTCCCCAG GGGCCTATTG GCTATCCTGG TCCTCGTGGC 
CTCAAGGGCG GTCAAGGAAT CCGGGGATTG AAGGGTCACA AAGGAGAGAA GGGAGAAGAT 
GGTTTCCCTG GAATTAAGGG AGACTTTGGG ATCAAGGGTG AGAGGGGTGA GATTGGAGTA 
CCAGGTCCTA GAGGAGAGGA CGGTCCAGAG GGGCCAAAAG GTCGTTTTGG TCCCCCTGGT 
GAAACTGGAC CACTCGGAGC AGTAGGAGAG AAGGGTAAAC TTGGTGTACC GGGACTTCCT 
GGATATCCTG GAAGACAAGG ACCAAAGGGA TCTCTTGGAT TCCCAGGATT CCCTGGATCC 
AATGGCGAGA AGGGAACAAG GGGTCTCACT GGCAAATCAG GACCAAGAGG ACAAAGAGGA 
CCAACGGGCC CCAGAGGGCA GAGGGGACCG AGGGGCGCAA CTGGCAAACT GGGGCCAAAG 
GGGACTTCGG GAAGTGATGG CCCCCCTGGT CCTCCTGGAG AAAGGGGCTT GCCTGGGCCT 
CAGGGAGCCA ATGGGTTCCC CGGACCTAAA GGGCCACCGG GGCCACCAGG GAAGGACGGG 
CTGCCTGGAC ACCCTGGGCA GAGAGGAGAA GTGGGTTTCC AAGGTAAAAT GGGTCCACCT 
GGGCCTCCTG GAGTTGTTGG ACCTCAGGGT CCAGCTGGAG AGACTGGCCC TAATGGTGAA 
CGTGGCCACC CTGGACCACC AGGTCCACCA GGAGAACAGG GACTTCCTGG TCCCTCAGGA 
AAGGAGGGAA CCAAAGGAGA CCCTGGACCC CCAGGAGGCC CTGGCAAAGA TGGGCCTCCT 
GGACTGAGGG GCTTCCCTGG TGAGAGAGGA CTGCCAGGAA CACCTGGAGG TGGAGGACTC 
AAAGGAAGCG AAGGACCTGC TGGACCTCCT GGACCGGCTG GCTCTCCTGG TGAGAGGGGG 
CAATCTGGTA CCGCTGGGCC TGTTGGACCT CCTGGAAGAC CAGGTCCTCA GGGACCTCCT 
GGACCTGCAG GAGAGAAAGG TGTTTCTGGT GAGAAAGGCC CAATTGGCCC AGCTGGTCGT 
GACGGACTAC AGGGTCCTGT GGGTCTGCCT GGCCCAGCTG GACCGCCCGG AGTACCTGGA 
GAAGATGGAG ATAAGGGGGA GGTTGGAGAA CACGGTCAGA AAGGTGCCAA GGGAGGGAAA 
GGAGAGCATG GTCCTCCAGG TCCACCTGGA CCAATGGGAC CAGTCGGCCA GCCTGGTGCT 
CCAGGTGCTG ATGGAGAGCT TGGGCCGAGG GGTCAGCAGG GACCTTTTGG AGCTAAAGGG 
GATGAAGGAA CCAGAGGATT CCCAGGAGCC CCAGGACCCA TTGGACTCCA GGGTTTGCCT 
GGGCCACCAG GTGAGAAGGG AGAAACCGGA GATGTTGGAC CTCTGGGACC TCCAGGCCCT 
CCAGGACCCC GTGGCCCTGC TGGACCCAAC GGTGCTGATG GCCCTCAAGG GCCTCCTGGT 
GGTTTGGGTA ATCCTGGACC TATCGGTGAG AAGGGAGAGC CTGGAGAGTC TGGACCCCCT 
GGAATTGGAG GGGAGCCTGG AAAGAAGGGC CCCAGAGGGG AACGTGGGGA GAAGGGGGAG 
GCTGGACAAC CAGGAACCGC TGGATCCACT GGAGGACGTG GACTACCTGG AGATGATGGA 
CCCAAAGGAA ACCCAGGTCC TGTTGGTTTC CCCGGTGATC CTGGTCCACC GGGTGAGGTT 
GGACCCAGAG GTCAAGACGG TGCCAAGGGA GAAAGAGGAG AAGATGGTGA AGCAGGAGAG 
TCTGGATCCC CTGGACCTCC CGGTGAGAAC GGACCCCCCG GCCCCCTAGG AAAGAGGGGT 
CCTGCTGGAA CAAGAGGACC AGAGGGACGC CAGGGAGAGA AGGGAACAAA GGGGGATCCA 
GGTGCTGTTG GCCCCCCAGG CAAAACAGGC CCTGTGGGTC CACAGGGAGT GCCAGGAAAA 
CCTGGAACAG AAGGCCTTAG AGGGCTTCCA GGATCAGTGG GTGAGCAAGG ATCTCCAGGA 
CCTGCAGGAC AGAAAGGACC ACCTGGTCCT ATTGGACCTC CCGGTTTACC TGGTCTGCGT 
GGAGATTCTG GCGGCAAAGG AGAAAAGGGA CACCCGGGTC TTATCGGTCT CATTGGACCC 
CCAGGAGAAC AGGGTGAGAA AGGAGACAGA GGCATGCCTG GGCCCCATGG ATCCAATGGA 
CCAAAAGGAG AGACTGGAAT GCCTGGAGGC ACAGGACCTA TCGGCCCCTC TGGTCCTCCT 
GGTCTGCCTG GTCCTCAAGG TATTAAAGGA GCCAAAGGTT CTTCTGGTGG AGCTGGTCCA 
AAAGGAGAAA AAGGAGTACA AGGACCCCCT GGACTTCCTG GCCCACCAGG GGAGGTTATT 
CAGCCACTAC CCATCCAAAG GAGTCCCAAG TCCAAGCGGT CCATTGATGC CAGCCAGCTA 
CTTCCTGAGA ATGACATGCC TGCATCCGAT GCCACCGGGA CAGAATTCCT GATGGGTAGT 
GAAGGCATGG AGGAGATCTT TGGCTCCCTC AACTCGCTTC GGCAGGAGAT TGAGACCATG 
CGCTTCCCTC TGGGCACCCA GGACAGCCCC GGCCGCACCT GCCAAGACCT GCACCTCAGT 
CAGCCAGAAC TCAAAGATGG AGAATACTGG ATTGACCCCA ACCAGGGCTG CTCCAGAGAC 
GCCTTCAAGG TCTTCTGTAA CTTTACTAAG GGGGCAGAGA CGTGCCTCTA CCCCAGCCAG 
AGCATCAGCA CGGTGAAGAT GAATTCCTGG AACAAAGAGA CTCCAGGTTC CTGGTACAGT 
CAGTTCTCCA CTGGCAGTAA GTTTTCATAC GTGGACTCCA ACGGCGAACC TGTGGGTGTG 
GTTCAGTTGG GTTTCCTTCG TCTCTTGAGC GTGCAGGCCC ACCAGACTCT CACATACCAC 
TGCCACCGCT CGGTAGCGTG GGCTGACCGC AACGCCAAGA ACACATACAG CCGAGCTCTG 
CACTTCAGGG GTGCCAACGA CGAGGATCTA AGCTATGAGA CCAATCCATA CGTCAAGGCT 
TTGGTTGATG GCTGCTCTTA TCGTAAGGGC TTTGACCGGA CAGTCCTGGA GATCAACACA 
CCACAGGTGG AACATCTTCC TCTGCTGGAT ATCAAGGTGT CAGACTTTGG GGAGAGCAAC 
CAGCAGTTTG GCTTTGAAGT GGGACCTGTC TGTTTCCAAG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615667.1
CDS	392..6856
Translation	MDTGDCPCRKKRRPWRMDFSSFALVTVMLIVCQTALVRADPVDVLRALQVPSLPEGVKKVPGFCTSRRSSSPDHAYRITKKAQISAPTKQLFSGRFPENFSIMALVKAQAGLQAFLLSIYSEQGIQQLGIEMGRSPVFLYEDQNGKPAPEDYPLFRGVNLADGKWHRIALSVSKKNVTLLLDCKKKMTRPLPRSNSAEVDTNGITVFGARLLDEEVFQGDIQQLLIASNPQAAYDFCEHYSPDCDSPLPKTQAQDPNTYKKQLNGKGAPTKSTAVKAKPTPAKPAKSGSYKLVVKSPMPIKAAKSTAKPFKGRPTAVEILLSKINPITPSKSTISLIKNGNGKSAAAKTNGLAKANNNGNGRATGAKVNGNGKADSKPTAQARVSGNGKVVVEKKVIINAKSSAEKKSSGNGKVTIVTKSNGAAEKKTSGSGKATIEVKEIKVKVHGKPENKVAGEAKANGNGKIITVVEKKFTNGAANRAKIETTTRAKTEKVIKVEKIEKTTLSTAKSATKLDASKAAKPTKATKSDATTLTKATKAAAAAGKTQARAEVKEVTQVKTVVTKVPLVKTTVSKPAKNVVERVTMPRKVASTSMPVRPTPPRPTKPKMAVDINVKSQHKPFPPFGKTALSGTTKKVTNGYQQDVDTEYSEAGHTPTERDYYYEETVPLPEGEVVGQEELAVQPQVEPVLEGEEVHSTKAVGEGEEAFTEEYVTGDVTLKEYDYSYRDYNEPLVETGEVDANMGPALSAVTDEGGAGVRGQKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPSGPPGSVGDPGERGPPGKAGLPGADGVPGPPGTSVMLPFRFGQSGGDKGPAVSAQEAQAAAILSQARMALKGPPGPMGFTGRPGPLGNPGSPGLKGESGDPGPQGPRGSLGLMGPPGKSGRRGRAGADGARGMPGESGSKGDRGFDGLPGLPGDKGHRGDSGPMGPPGSQGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGIPGPPGVRGSDGPHGPKGNLGPQGEPGPPGQQGTPGTQGMPGPQGHTGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGPIGYPGPRGLKGGQGIRGLKGHKGEKGEDGFPGIKGDFGIKGERGEIGVPGPRGEDGPEGPKGRFGPPGETGPLGAVGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGSNGEKGTRGLTGKSGPRGQRGPTGPRGQRGPRGATGKLGPKGTSGSDGPPGPPGERGLPGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKMGPPGPPGVVGPQGPAGETGPNGERGHPGPPGPPGEQGLPGPSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGGGGLKGSEGPAGPPGPAGSPGERGQSGTAGPVGPPGRPGPQGPPGPAGEKGVSGEKGPIGPAGRDGLQGPVGLPGPAGPPGVPGEDGDKGEVGEHGQKGAKGGKGEHGPPGPPGPMGPVGQPGAPGADGELGPRGQQGPFGAKGDEGTRGFPGAPGPIGLQGLPGPPGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGIGGEPGKKGPRGERGEKGEAGQPGTAGSTGGRGLPGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGERGEDGEAGESGSPGPPGENGPPGPLGKRGPAGTRGPEGRQGEKGTKGDPGAVGPPGKTGPVGPQGVPGKPGTEGLRGLPGSVGEQGSPGPAGQKGPPGPIGPPGLPGLRGDSGGKGEKGHPGLIGLIGPPGEQGEKGDRGMPGPHGSNGPKGETGMPGGTGPIGPSGPPGLPGPQGIKGAKGSSGGAGPKGEKGVQGPPGLPGPPGEVIQPLPIQRSPKSKRSIDASQLLPENDMPASDATGTEFLMGSEGMEEIFGSLNSLRQEIETMRFPLGTQDSPGRTCQDLHLSQPELKDGEYWIDPNQGCSRDAFKVFCNFTKGAETCLYPSQSISTVKMNSWNKETPGSWYSQFSTGSKFSYVDSNGEPVGVVQLGFLRLLSVQAHQTLTYHCHRSVAWADRNAKNTYSRALHFRGANDEDLSYETNPYVKALVDGCSYRKGFDRTVLEINTPQVEHLPLLDIKVSDFGESNQQFGFEVGPVCFQG

Target ORF information:

RefSeq Version	XM_011617365.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617365.1

ORF Insert Sequence:

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGGC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATAAG 
AAGCAGCTCA ATGGAAAAGG AGCCCCCACT AAATCCACTG CTGTTAAAGC AAAACCTACC 
CCAGCAAAGC CAGCCAAGTC TGGATCATAT AAGCTTGTAG TCAAGTCCCC TATGCCCATC 
AAAGCAGCAA AGTCTACAGC CAAGCCATTT AAAGGCAGGC CCACTGCAGT AGAGATCCTT 
CTCTCTAAGA TAAACCCAAT AACACCATCT AAATCCACAA TTTCCTTGAT TAAAAATGGC 
AACGGTAAAT CAGCTGCAGC AAAGACAAAT GGATTGGCCA AAGCCAACAA CAATGGAAAT 
GGAAGAGCAA CAGGCGCTAA AGTAAATGGC AATGGCAAAG CTGATTCTAA GCCCACAGCT 
CAGGCCAGAG TGAGCGGAAA TGGCAAAGTT GTAGTGGAGA AAAAAGTCAT CATAAATGCC 
AAATCTTCAG CAGAAAAGAA ATCCAGCGGA AATGGAAAAG TCACCATTGT AACCAAGAGT 
AATGGTGCAG CAGAGAAGAA AACCAGTGGT AGTGGCAAAG CTACCATTGA GGTTAAAGAA 
ATTAAAGTCA AAGTTCATGG AAAACCTGAA AATAAAGTTG CAGGTGAGGC CAAGGCTAAC 
GGCAATGGTA AAATTATCAC TGTGGTAGAA AAGAAATTCA CCAATGGTGC TGCGAATCGG 
GCAAAGATTG AAACCACTAC AAGAGCAAAG ACGGAGAAAG TTATCAAAGT TGAAAAGATA 
GAAAAGACTA CGCTCAGTAC AGCTAAATCA GCCACAAAAC TAGATGCTTC TAAAGCAGCC 
AAACCCACAA AAGCCACAAA ATCTGATGCT ACAACATTAA CAAAGGCCAC CAAAGCAGCG 
GCAGCAGCAG GAAAGACCCA AGCAAGGGCA GAGGTCAAAG AAGTCACCCA AGTTAAAACT 
GTTGTCACCA AAGTCCCTCT GGTCAAAACC ACTGTGAGCA AACCAGCGAA GAATGTGGTG 
GAGAGAGTTA CGATGCCCAG GAAAGTAGCC TCGACGTCCA TGCCCGTGAG GCCCACTCCA 
CCCAGACCTA CGAAACCCAA GATGGCGGTG GACATTAATG TCAAATCCCA GCACAAACCT 
TTCCCACCAT TTGGCAAGAC TGCGTTGAGT GGAACTACGA AGAAAGTTAC CAACGGTTAT 
CAGCAGGATG TAGACACTGA ATATTCCGAG GCTGGCCACA CCCCTACAGA GAGAGATTAT 
TACTATGAGG AGACGGTACC ACTGCCAGAG GGTGAGGTGG TTGGGCAAGA GGAACTCGCC 
GTACAGCCCC AGGTGGAGCC TGTGTTGGAG GGAGAAGAGG TGCATTCGAC CAAGGCTGTC 
GGTGAGGGAG AGGAGGCCTT CACAGAGGAG TATGTGACTG GAGATGTGAC CCTTAAGGAA 
TACGACTATT CCTACAGGGA CTACAATGAG CCATTAGTGG AGACTGGAGA AGTCGATGCC 
AACATGGGCC CCGCCCTCTC TGCTGTGACA GACGAGGGAG GCGCTGGGGT GAGAGGCCAG 
AAAGGAGAGA AAGGAGAACC TGCTGTTTTG GAGCCTGGGA TGCTGATTGA AGGACCACCA 
GGACCTGAGG GACCTGCTGG CCTACCTGGT CCTTCTGGCC CTTCTGGACC TCCTGGCTCT 
GTGGGTGACC CAGGAGAGAG GGGCCCTCCT GGTAAGGCTG GTCTTCCTGG AGCTGATGGA 
GTCCCTGGAC CTCCTGGGAC CTCTGTTATG CTGCCTTTCC GTTTTGGTCA GAGTGGAGGA 
GATAAAGGTC CTGCTGTTTC TGCTCAGGAA GCTCAGGCAG CAGCCATCTT GTCTCAGGCC 
AGGATGGCTC TTAAAGGACC TCCTGGTCCA ATGGGATTCA CTGGGCGTCC CGGACCCTTG 
GGAAATCCTG GAAGTCCTGG TCTGAAAGGA GAGAGTGGAG ACCCTGGACC CCAGGGCCCG 
AGGGGATCTC TAGGCTTGAT GGGACCTCCA GGCAAATCAG GAAGAAGAGG TCGCGCTGGA 
GCCGACGGTG CCAGAGGAAT GCCTGGAGAG TCTGGTTCTA AGGGTGATCG TGGTTTTGAT 
GGTCTTCCTG GTTTACCTGG AGACAAAGGA CACAGGGGTG ACTCGGGACC AATGGGACCT 
CCAGGATCTC AGGGAGAGGA TGGAGAAAGG GGAGACGACG GAGACGTTGG ACCCCGGGGT 
CTTCCAGGTG AACCAGGACC TCGTGGTTTG CTTGGACCTA AAGGTCCTCC TGGAATCCCA 
GGCCCTCCTG GTGTACGAGG AAGTGACGGA CCCCACGGTC CAAAAGGAAA CTTGGGTCCT 
CAAGGTGAGC CAGGACCCCC TGGTCAGCAG GGAACCCCAG GAACTCAGGG AATGCCAGGA 
CCTCAAGGAC ATACTGGACC TCCAGGAGAA AAGGGTCCAA CAGGAAAACC GGGTTTACCA 
GGAATGCCTG GAGCTGATGG CCCTCCTGGT CACCCAGGAA AGGAAGGCCC ATCAGGCACC 
AAAGGAAACC AGGGTCCCAG TGGTCCCCAG GGGCCTATTG GCTATCCTGG TCCTCGTGGC 
CTCAAGGGCG GTCAAGGAAT CCGGGGATTG AAGGGTCACA AAGGAGAGAA GGGAGAAGAT 
GGTTTCCCTG GAATTAAGGG AGACTTTGGG ATCAAGGGTG AGAGGGGTGA GATTGGAGTA 
CCAGGTCCTA GAGGAGAGGA CGGTCCAGAG GGGCCAAAAG GTCGTTTTGG TCCCCCTGGT 
GAAACTGGAC CACTCGGAGC AGTAGGAGAG AAGGGTAAAC TTGGTGTACC GGGACTTCCT 
GGATATCCTG GAAGACAAGG ACCAAAGGGA TCTCTTGGAT TCCCAGGATT CCCTGGATCC 
AATGGCGAGA AGGGAACAAG GGGTCTCACT GGCAAATCAG GACCAAGAGG ACAAAGAGGA 
CCAACGGGCC CCAGAGGGCA GAGGGGACCG AGGGGCGCAA CTGGCAAACT GGGGCCAAAG 
GGGACTTCGG GAAGTGATGG CCCCCCTGGT CCTCCTGGAG AAAGGGGCTT GCCTGGGCCT 
CAGGGAGCCA ATGGGTTCCC CGGACCTAAA GGGCCACCGG GGCCACCAGG GAAGGACGGG 
CTGCCTGGAC ACCCTGGGCA GAGAGGAGAA GTGGGTTTCC AAGGTAAAAT GGGTCCACCT 
GGGCCTCCTG GAGTTGTTGG ACCTCAGGGT CCAGCTGGAG AGACTGGCCC TAATGGTGAA 
CGTGGCCACC CTGGACCACC AGGTCCACCA GGAGAACAGG GACTTCCTGG TCCCTCAGGA 
AAGGAGGGAA CCAAAGGAGA CCCTGGACCC CCAGGAGGCC CTGGCAAAGA TGGGCCTCCT 
GGACTGAGGG GCTTCCCTGG TGAGAGAGGA CTGCCAGGAA CACCTGGAGG TGGAGGACTC 
AAAGGAAGCG AAGGACCTGC TGGACCTCCT GGACCGGCTG GCTCTCCTGG TGAGAGGGGG 
CAATCTGGTA CCGCTGGGCC TGTTGGACCT CCTGGAAGAC CAGGTCCTCA GGGACCTCCT 
GGACCTGCAG GAGAGAAAGG TGTTTCTGGT GAGAAAGGCC CAATTGGCCC AGCTGGTCGT 
GACGGACTAC AGGGTCCTGT GGGTCTGCCT GGCCCAGCTG GACCGCCCGG AGTACCTGGA 
GAAGATGGAG ATAAGGGGGA GGTTGGAGAA CACGGTCAGA AAGGTGCCAA GGGAGGGAAA 
GGAGAGCATG GTCCTCCAGG TCCACCTGGA CCAATGGGAC CAGTCGGCCA GCCTGGTGCT 
CCAGGTGCTG ATGGAGAGCT TGGGCCGAGG GGTCAGCAGG GACCTTTTGG AGCTAAAGGG 
GATGAAGGAA CCAGAGGATT CCCAGGAGCC CCAGGACCCA TTGGACTCCA GGGTTTGCCT 
GGGCCACCAG GTGAGAAGGG AGAAACCGGA GATGTTGGAC CTCTGGGACC TCCAGGCCCT 
CCAGGACCCC GTGGCCCTGC TGGACCCAAC GGTGCTGATG GCCCTCAAGG GCCTCCTGGT 
GGTTTGGGTA ATCCTGGACC TATCGGTGAG AAGGGAGAGC CTGGAGAGTC TGGACCCCCT 
GGAATTGGAG GGGAGCCTGG AAAGAAGGGC CCCAGAGGGG AACGTGGGGA GAAGGGGGAG 
GCTGGACAAC CAGGAACCGC TGGATCCACT GGAGGACGTG GACTACCTGG AGATGATGGA 
CCCAAAGGAA ACCCAGGTCC TGTTGGTTTC CCCGGTGATC CTGGTCCACC GGGTGAGGTT 
GGACCCAGAG GTCAAGACGG TGCCAAGGGA GAAAGAGGAG AAGATGGTGA AGCAGGAGAG 
TCTGGATCCC CTGGACCTCC CGGTGAGAAC GGACCCCCCG GCCCCCTAGG AAAGAGGGGT 
CCTGCTGGAA CAAGAGGACC AGAGGGACGC CAGGGAGAGA AGGGAACAAA GGGGGATCCA 
GGTGCTGTTG GCCCCCCAGG CAAAACAGGC CCTGTGGGTC CACAGGGAGT GCCAGGAAAA 
CCTGGAACAG AAGGCCTTAG AGGGCTTCCA GGATCAGTGG GTGAGCAAGG ATCTCCAGGA 
CCTGCAGGAC AGAAAGGACC ACCTGGTCCT ATTGGACCTC CCGGTTTACC TGGTCTGCGT 
GGAGATTCTG GCGGCAAAGG AGAAAAGGGA CACCCGGGTC TTATCGGTCT CATTGGACCC 
CCAGGAGAAC AGGGTGAGAA AGGAGACAGA GGCATGCCTG GGCCCCATGG ATCCAATGGA 
CCAAAAGGAG AGACTGGAAT GCCTGGAGGC ACAGGACCTA TCGGCCCCTC TGGTCCTCCT 
GGTCTGCCTG GTCCTCAAGG TATTAAAGGA GCCAAAGGTT CTTCTGGTGG AGCTGGTCCA 
AAAGGAGAAA AAGGAGTACA AGGACCCCCT GGACTTCCTG GCCCACCAGG GGAGGTTATT 
CAGCCACTAC CCATCCAAAG GAGTCCCAAG TCCAAGCGGT CCATTGATGC CAGCCAGCTA 
CTTCCTGAGA ATGACATGCC TGCATCCGAT GCCACCGGGA CAGAATTCCT GATGGGTAGT 
GAAGGCATGG AGGAGATCTT TGGCTCCCTC AACTCGCTTC GGCAGGAGAT TGAGACCATG 
CGCTTCCCTC TGGGCACCCA GGACAGCCCC GGCCGCACCT GCCAAGACCT GCACCTCAGT 
CAGCCAGAAC TCAAAGATGG AGAATACTGG ATTGACCCCA ACCAGGGCTG CTCCAGAGAC 
GCCTTCAAGG TCTTCTGTAA CTTTACTAAG GGGGCAGAGA CGTGCCTCTA CCCCAGCCAG 
AGCATCAGCA CGGTGAAGAT GAATTCCTGG AACAAAGAGA CTCCAGGTTC CTGGTACAGT 
CAGTTCTCCA CTGGCAGTAA GTTTTCATAC GTGGACTCCA ACGGCGAACC TGTGGGTGTG 
GTTCAGTTGG GTTTCCTTCG TCTCTTGAGC GTGCAGGCCC ACCAGACTCT CACATACCAC 
TGCCACCGCT CGGTAGCGTG GGCTGACCGC AACGCCAAGA ACACATACAG CCGAGCTCTG 
CACTTCAGGG GTGCCAACGA CGAGGATCTA AGCTATGAGA CCAATCCATA CGTCAAGGCT 
TTGGTTGATG GCTGCTCTTA TCGTAAGGGC TTTGACCGGA CAGTCCTGGA GATCAACACA 
CCACAGGTGG AACATCTTCC TCTGCTGGAT ATCAAGGTGT CAGACTTTGG GGAGAGCAAC 
CAGCAGTTTG GCTTTGAAGT GGGACCTGTC TGTTTCCAAG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101066527 ( XM_011617366.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011615668.1 Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X2, mRNA.

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CloneID	OTo22617
Clone ID Related Accession (Same CDS sequence)	XM_011617366.1
Accession Version	XM_011617366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	collagen alpha-2(XI) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071261.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGGC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATAAG 
AAGCAGCTCA ATGGAAAAGG AGCCCCCACT AAATCCACTG CTGTTAAAGC AAAACCTACC 
CCAGCAAAGC CAGCCAAGTC TGGATCATAT AAGCTTGTAG TCAAGTCCCC TATGCCCATC 
AAAGCAGCAA AGTCTACAGC CAAGCCATTT AAAGGCAGGC CCACTGCAGT AGAGATCCTT 
CTCTCTAAGA TAAACCCAAT AACACCATCT AAATCCACAA TTTCCTTGAT TAAAAATGGC 
AACGGTAAAT CAGCTGCAGC AAAGACAAAT GGATTGGCCA AAGCCAACAA CAATGGAAAT 
GGAAGAGCAA CAGGCGCTAA AGTAAATGGC AATGGCAAAG CTGATTCTAA GCCCACAGCT 
CAGGCCAGAG TGAGCGGAAA TGGCAAAGTT GTAGTGGAGA AAAAAGTCAT CATAAATGCC 
AAATCTTCAG CAGAAAAGAA ATCCAGCGGA AATGGAAAAG TCACCATTGT AACCAAGAGT 
AATGGTGCAG CAGAGAAGAA AACCAGTGGT AGTGGCAAAG CTACCATTGA GGTTAAAGAA 
ATTAAAGTCA AAGTTCATGG AAAACCTGAA AATAAAGTTG CAGGTGAGGC CAAGGCTAAC 
GGCAATGGTA AAATTATCAC TGTGGTAGAA AAGAAATTCA CCAATGGTGC TGCGAATCGG 
GCAAAGATTG AAACCACTAC AAGAGCAAAG ACGGAGAAAG TTATCAAAGT TGAAAAGATA 
GAAAAGACTA CGCTCAGTAC AGCTAAATCA GCCACAAAAC TAGATGCTTC TAAAGCAGCC 
AAACCCACAA AAGCCACAAA ATCTGATGCT ACAACATTAA CAAAGGCCAC CAAAGCAGCG 
GCAGCAGCAG GAAAGACCCA AGCAAGGGCA GAGGTCAAAG AAGTCACCCA AGTTAAAACT 
GTTGTCACCA AAGTCCCTCT GGTCAAAACC ACTGTGAGCA AACCAGCGAA GAATGTGGTG 
GAGAGAGTTA CGATGCCCAG GAAAGTAGCC TCGACGTCCA TGCCCGTGAG GCCCACTCCA 
CCCAGACCTA CGAAACCCAA GATGGCGGTG GACATTAATG TCAAATCCCA GCACAAACCT 
TTCCCACCAT TTGGCAAGAC TGCGTTGAGT GGAACTACGA AGAAAGTTAC CAACGGTTAT 
CAGCAGCCCC AGGTGGAGCC TGTGTTGGAG GGAGAAGAGG TGCATTCGAC CAAGGCTGTC 
GGTGAGGGAG AGGAGGCCTT CACAGAGGAG TATGTGACTG GAGATGTGAC CCTTAAGGAA 
TACGACTATT CCTACAGGGA CTACAATGAG CCATTAGTGG AGACTGGAGA AGTCGATGCC 
AACATGGGCC CCGCCCTCTC TGCTGTGACA GACGAGGGAG GCGCTGGGGT GAGAGGCCAG 
AAAGGAGAGA AAGGAGAACC TGCTGTTTTG GAGCCTGGGA TGCTGATTGA AGGACCACCA 
GGACCTGAGG GACCTGCTGG CCTACCTGGT CCTTCTGGCC CTTCTGGACC TCCTGGCTCT 
GTGGGTGACC CAGGAGAGAG GGGCCCTCCT GGTAAGGCTG GTCTTCCTGG AGCTGATGGA 
GTCCCTGGAC CTCCTGGGAC CTCTGTTATG CTGCCTTTCC GTTTTGGTCA GAGTGGAGGA 
GATAAAGGTC CTGCTGTTTC TGCTCAGGAA GCTCAGGCAG CAGCCATCTT GTCTCAGGCC 
AGGATGGCTC TTAAAGGACC TCCTGGTCCA ATGGGATTCA CTGGGCGTCC CGGACCCTTG 
GGAAATCCTG GAAGTCCTGG TCTGAAAGGA GAGAGTGGAG ACCCTGGACC CCAGGGCCCG 
AGGGGATCTC TAGGCTTGAT GGGACCTCCA GGCAAATCAG GAAGAAGAGG TCGCGCTGGA 
GCCGACGGTG CCAGAGGAAT GCCTGGAGAG TCTGGTTCTA AGGGTGATCG TGGTTTTGAT 
GGTCTTCCTG GTTTACCTGG AGACAAAGGA CACAGGGGTG ACTCGGGACC AATGGGACCT 
CCAGGATCTC AGGGAGAGGA TGGAGAAAGG GGAGACGACG GAGACGTTGG ACCCCGGGGT 
CTTCCAGGTG AACCAGGACC TCGTGGTTTG CTTGGACCTA AAGGTCCTCC TGGAATCCCA 
GGCCCTCCTG GTGTACGAGG AAGTGACGGA CCCCACGGTC CAAAAGGAAA CTTGGGTCCT 
CAAGGTGAGC CAGGACCCCC TGGTCAGCAG GGAACCCCAG GAACTCAGGG AATGCCAGGA 
CCTCAAGGAC ATACTGGACC TCCAGGAGAA AAGGGTCCAA CAGGAAAACC GGGTTTACCA 
GGAATGCCTG GAGCTGATGG CCCTCCTGGT CACCCAGGAA AGGAAGGCCC ATCAGGCACC 
AAAGGAAACC AGGGTCCCAG TGGTCCCCAG GGGCCTATTG GCTATCCTGG TCCTCGTGGC 
CTCAAGGGCG GTCAAGGAAT CCGGGGATTG AAGGGTCACA AAGGAGAGAA GGGAGAAGAT 
GGTTTCCCTG GAATTAAGGG AGACTTTGGG ATCAAGGGTG AGAGGGGTGA GATTGGAGTA 
CCAGGTCCTA GAGGAGAGGA CGGTCCAGAG GGGCCAAAAG GTCGTTTTGG TCCCCCTGGT 
GAAACTGGAC CACTCGGAGC AGTAGGAGAG AAGGGTAAAC TTGGTGTACC GGGACTTCCT 
GGATATCCTG GAAGACAAGG ACCAAAGGGA TCTCTTGGAT TCCCAGGATT CCCTGGATCC 
AATGGCGAGA AGGGAACAAG GGGTCTCACT GGCAAATCAG GACCAAGAGG ACAAAGAGGA 
CCAACGGGCC CCAGAGGGCA GAGGGGACCG AGGGGCGCAA CTGGCAAACT GGGGCCAAAG 
GGGACTTCGG GAAGTGATGG CCCCCCTGGT CCTCCTGGAG AAAGGGGCTT GCCTGGGCCT 
CAGGGAGCCA ATGGGTTCCC CGGACCTAAA GGGCCACCGG GGCCACCAGG GAAGGACGGG 
CTGCCTGGAC ACCCTGGGCA GAGAGGAGAA GTGGGTTTCC AAGGTAAAAT GGGTCCACCT 
GGGCCTCCTG GAGTTGTTGG ACCTCAGGGT CCAGCTGGAG AGACTGGCCC TAATGGTGAA 
CGTGGCCACC CTGGACCACC AGGTCCACCA GGAGAACAGG GACTTCCTGG TCCCTCAGGA 
AAGGAGGGAA CCAAAGGAGA CCCTGGACCC CCAGGAGGCC CTGGCAAAGA TGGGCCTCCT 
GGACTGAGGG GCTTCCCTGG TGAGAGAGGA CTGCCAGGAA CACCTGGAGG TGGAGGACTC 
AAAGGAAGCG AAGGACCTGC TGGACCTCCT GGACCGGCTG GCTCTCCTGG TGAGAGGGGG 
CAATCTGGTA CCGCTGGGCC TGTTGGACCT CCTGGAAGAC CAGGTCCTCA GGGACCTCCT 
GGACCTGCAG GAGAGAAAGG TGTTTCTGGT GAGAAAGGCC CAATTGGCCC AGCTGGTCGT 
GACGGACTAC AGGGTCCTGT GGGTCTGCCT GGCCCAGCTG GACCGCCCGG AGTACCTGGA 
GAAGATGGAG ATAAGGGGGA GGTTGGAGAA CACGGTCAGA AAGGTGCCAA GGGAGGGAAA 
GGAGAGCATG GTCCTCCAGG TCCACCTGGA CCAATGGGAC CAGTCGGCCA GCCTGGTGCT 
CCAGGTGCTG ATGGAGAGCT TGGGCCGAGG GGTCAGCAGG GACCTTTTGG AGCTAAAGGG 
GATGAAGGAA CCAGAGGATT CCCAGGAGCC CCAGGACCCA TTGGACTCCA GGGTTTGCCT 
GGGCCACCAG GTGAGAAGGG AGAAACCGGA GATGTTGGAC CTCTGGGACC TCCAGGCCCT 
CCAGGACCCC GTGGCCCTGC TGGACCCAAC GGTGCTGATG GCCCTCAAGG GCCTCCTGGT 
GGTTTGGGTA ATCCTGGACC TATCGGTGAG AAGGGAGAGC CTGGAGAGTC TGGACCCCCT 
GGAATTGGAG GGGAGCCTGG AAAGAAGGGC CCCAGAGGGG AACGTGGGGA GAAGGGGGAG 
GCTGGACAAC CAGGAACCGC TGGATCCACT GGAGGACGTG GACTACCTGG AGATGATGGA 
CCCAAAGGAA ACCCAGGTCC TGTTGGTTTC CCCGGTGATC CTGGTCCACC GGGTGAGGTT 
GGACCCAGAG GTCAAGACGG TGCCAAGGGA GAAAGAGGAG AAGATGGTGA AGCAGGAGAG 
TCTGGATCCC CTGGACCTCC CGGTGAGAAC GGACCCCCCG GCCCCCTAGG AAAGAGGGGT 
CCTGCTGGAA CAAGAGGACC AGAGGGACGC CAGGGAGAGA AGGGAACAAA GGGGGATCCA 
GGTGCTGTTG GCCCCCCAGG CAAAACAGGC CCTGTGGGTC CACAGGGAGT GCCAGGAAAA 
CCTGGAACAG AAGGCCTTAG AGGGCTTCCA GGATCAGTGG GTGAGCAAGG ATCTCCAGGA 
CCTGCAGGAC AGAAAGGACC ACCTGGTCCT ATTGGACCTC CCGGTTTACC TGGTCTGCGT 
GGAGATTCTG GCGGCAAAGG AGAAAAGGGA CACCCGGGTC TTATCGGTCT CATTGGACCC 
CCAGGAGAAC AGGGTGAGAA AGGAGACAGA GGCATGCCTG GGCCCCATGG ATCCAATGGA 
CCAAAAGGAG AGACTGGAAT GCCTGGAGGC ACAGGACCTA TCGGCCCCTC TGGTCCTCCT 
GGTCTGCCTG GTCCTCAAGG TATTAAAGGA GCCAAAGGTT CTTCTGGTGG AGCTGGTCCA 
AAAGGAGAAA AAGGAGTACA AGGACCCCCT GGACTTCCTG GCCCACCAGG GGAGGTTATT 
CAGCCACTAC CCATCCAAAG GAGTCCCAAG TCCAAGCGGT CCATTGATGC CAGCCAGCTA 
CTTCCTGAGA ATGACATGCC TGCATCCGAT GCCACCGGGA CAGAATTCCT GATGGGTAGT 
GAAGGCATGG AGGAGATCTT TGGCTCCCTC AACTCGCTTC GGCAGGAGAT TGAGACCATG 
CGCTTCCCTC TGGGCACCCA GGACAGCCCC GGCCGCACCT GCCAAGACCT GCACCTCAGT 
CAGCCAGAAC TCAAAGATGG AGAATACTGG ATTGACCCCA ACCAGGGCTG CTCCAGAGAC 
GCCTTCAAGG TCTTCTGTAA CTTTACTAAG GGGGCAGAGA CGTGCCTCTA CCCCAGCCAG 
AGCATCAGCA CGGTGAAGAT GAATTCCTGG AACAAAGAGA CTCCAGGTTC CTGGTACAGT 
CAGTTCTCCA CTGGCAGTAA GTTTTCATAC GTGGACTCCA ACGGCGAACC TGTGGGTGTG 
GTTCAGTTGG GTTTCCTTCG TCTCTTGAGC GTGCAGGCCC ACCAGACTCT CACATACCAC 
TGCCACCGCT CGGTAGCGTG GGCTGACCGC AACGCCAAGA ACACATACAG CCGAGCTCTG 
CACTTCAGGG GTGCCAACGA CGAGGATCTA AGCTATGAGA CCAATCCATA CGTCAAGGCT 
TTGGTTGATG GCTGCTCTTA TCGTAAGGGC TTTGACCGGA CAGTCCTGGA GATCAACACA 
CCACAGGTGG AACATCTTCC TCTGCTGGAT ATCAAGGTGT CAGACTTTGG GGAGAGCAAC 
CAGCAGTTTG GCTTTGAAGT GGGACCTGTC TGTTTCCAAG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615668.1
CDS	392..6736
Translation	MDTGDCPCRKKRRPWRMDFSSFALVTVMLIVCQTALVRADPVDVLRALQVPSLPEGVKKVPGFCTSRRSSSPDHAYRITKKAQISAPTKQLFSGRFPENFSIMALVKAQAGLQAFLLSIYSEQGIQQLGIEMGRSPVFLYEDQNGKPAPEDYPLFRGVNLADGKWHRIALSVSKKNVTLLLDCKKKMTRPLPRSNSAEVDTNGITVFGARLLDEEVFQGDIQQLLIASNPQAAYDFCEHYSPDCDSPLPKTQAQDPNTYKKQLNGKGAPTKSTAVKAKPTPAKPAKSGSYKLVVKSPMPIKAAKSTAKPFKGRPTAVEILLSKINPITPSKSTISLIKNGNGKSAAAKTNGLAKANNNGNGRATGAKVNGNGKADSKPTAQARVSGNGKVVVEKKVIINAKSSAEKKSSGNGKVTIVTKSNGAAEKKTSGSGKATIEVKEIKVKVHGKPENKVAGEAKANGNGKIITVVEKKFTNGAANRAKIETTTRAKTEKVIKVEKIEKTTLSTAKSATKLDASKAAKPTKATKSDATTLTKATKAAAAAGKTQARAEVKEVTQVKTVVTKVPLVKTTVSKPAKNVVERVTMPRKVASTSMPVRPTPPRPTKPKMAVDINVKSQHKPFPPFGKTALSGTTKKVTNGYQQPQVEPVLEGEEVHSTKAVGEGEEAFTEEYVTGDVTLKEYDYSYRDYNEPLVETGEVDANMGPALSAVTDEGGAGVRGQKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPSGPPGSVGDPGERGPPGKAGLPGADGVPGPPGTSVMLPFRFGQSGGDKGPAVSAQEAQAAAILSQARMALKGPPGPMGFTGRPGPLGNPGSPGLKGESGDPGPQGPRGSLGLMGPPGKSGRRGRAGADGARGMPGESGSKGDRGFDGLPGLPGDKGHRGDSGPMGPPGSQGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGIPGPPGVRGSDGPHGPKGNLGPQGEPGPPGQQGTPGTQGMPGPQGHTGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGPIGYPGPRGLKGGQGIRGLKGHKGEKGEDGFPGIKGDFGIKGERGEIGVPGPRGEDGPEGPKGRFGPPGETGPLGAVGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGSNGEKGTRGLTGKSGPRGQRGPTGPRGQRGPRGATGKLGPKGTSGSDGPPGPPGERGLPGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKMGPPGPPGVVGPQGPAGETGPNGERGHPGPPGPPGEQGLPGPSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGGGGLKGSEGPAGPPGPAGSPGERGQSGTAGPVGPPGRPGPQGPPGPAGEKGVSGEKGPIGPAGRDGLQGPVGLPGPAGPPGVPGEDGDKGEVGEHGQKGAKGGKGEHGPPGPPGPMGPVGQPGAPGADGELGPRGQQGPFGAKGDEGTRGFPGAPGPIGLQGLPGPPGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGIGGEPGKKGPRGERGEKGEAGQPGTAGSTGGRGLPGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGERGEDGEAGESGSPGPPGENGPPGPLGKRGPAGTRGPEGRQGEKGTKGDPGAVGPPGKTGPVGPQGVPGKPGTEGLRGLPGSVGEQGSPGPAGQKGPPGPIGPPGLPGLRGDSGGKGEKGHPGLIGLIGPPGEQGEKGDRGMPGPHGSNGPKGETGMPGGTGPIGPSGPPGLPGPQGIKGAKGSSGGAGPKGEKGVQGPPGLPGPPGEVIQPLPIQRSPKSKRSIDASQLLPENDMPASDATGTEFLMGSEGMEEIFGSLNSLRQEIETMRFPLGTQDSPGRTCQDLHLSQPELKDGEYWIDPNQGCSRDAFKVFCNFTKGAETCLYPSQSISTVKMNSWNKETPGSWYSQFSTGSKFSYVDSNGEPVGVVQLGFLRLLSVQAHQTLTYHCHRSVAWADRNAKNTYSRALHFRGANDEDLSYETNPYVKALVDGCSYRKGFDRTVLEINTPQVEHLPLLDIKVSDFGESNQQFGFEVGPVCFQG

Target ORF information:

RefSeq Version	XM_011617366.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617366.1

ORF Insert Sequence:

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGGC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATAAG 
AAGCAGCTCA ATGGAAAAGG AGCCCCCACT AAATCCACTG CTGTTAAAGC AAAACCTACC 
CCAGCAAAGC CAGCCAAGTC TGGATCATAT AAGCTTGTAG TCAAGTCCCC TATGCCCATC 
AAAGCAGCAA AGTCTACAGC CAAGCCATTT AAAGGCAGGC CCACTGCAGT AGAGATCCTT 
CTCTCTAAGA TAAACCCAAT AACACCATCT AAATCCACAA TTTCCTTGAT TAAAAATGGC 
AACGGTAAAT CAGCTGCAGC AAAGACAAAT GGATTGGCCA AAGCCAACAA CAATGGAAAT 
GGAAGAGCAA CAGGCGCTAA AGTAAATGGC AATGGCAAAG CTGATTCTAA GCCCACAGCT 
CAGGCCAGAG TGAGCGGAAA TGGCAAAGTT GTAGTGGAGA AAAAAGTCAT CATAAATGCC 
AAATCTTCAG CAGAAAAGAA ATCCAGCGGA AATGGAAAAG TCACCATTGT AACCAAGAGT 
AATGGTGCAG CAGAGAAGAA AACCAGTGGT AGTGGCAAAG CTACCATTGA GGTTAAAGAA 
ATTAAAGTCA AAGTTCATGG AAAACCTGAA AATAAAGTTG CAGGTGAGGC CAAGGCTAAC 
GGCAATGGTA AAATTATCAC TGTGGTAGAA AAGAAATTCA CCAATGGTGC TGCGAATCGG 
GCAAAGATTG AAACCACTAC AAGAGCAAAG ACGGAGAAAG TTATCAAAGT TGAAAAGATA 
GAAAAGACTA CGCTCAGTAC AGCTAAATCA GCCACAAAAC TAGATGCTTC TAAAGCAGCC 
AAACCCACAA AAGCCACAAA ATCTGATGCT ACAACATTAA CAAAGGCCAC CAAAGCAGCG 
GCAGCAGCAG GAAAGACCCA AGCAAGGGCA GAGGTCAAAG AAGTCACCCA AGTTAAAACT 
GTTGTCACCA AAGTCCCTCT GGTCAAAACC ACTGTGAGCA AACCAGCGAA GAATGTGGTG 
GAGAGAGTTA CGATGCCCAG GAAAGTAGCC TCGACGTCCA TGCCCGTGAG GCCCACTCCA 
CCCAGACCTA CGAAACCCAA GATGGCGGTG GACATTAATG TCAAATCCCA GCACAAACCT 
TTCCCACCAT TTGGCAAGAC TGCGTTGAGT GGAACTACGA AGAAAGTTAC CAACGGTTAT 
CAGCAGCCCC AGGTGGAGCC TGTGTTGGAG GGAGAAGAGG TGCATTCGAC CAAGGCTGTC 
GGTGAGGGAG AGGAGGCCTT CACAGAGGAG TATGTGACTG GAGATGTGAC CCTTAAGGAA 
TACGACTATT CCTACAGGGA CTACAATGAG CCATTAGTGG AGACTGGAGA AGTCGATGCC 
AACATGGGCC CCGCCCTCTC TGCTGTGACA GACGAGGGAG GCGCTGGGGT GAGAGGCCAG 
AAAGGAGAGA AAGGAGAACC TGCTGTTTTG GAGCCTGGGA TGCTGATTGA AGGACCACCA 
GGACCTGAGG GACCTGCTGG CCTACCTGGT CCTTCTGGCC CTTCTGGACC TCCTGGCTCT 
GTGGGTGACC CAGGAGAGAG GGGCCCTCCT GGTAAGGCTG GTCTTCCTGG AGCTGATGGA 
GTCCCTGGAC CTCCTGGGAC CTCTGTTATG CTGCCTTTCC GTTTTGGTCA GAGTGGAGGA 
GATAAAGGTC CTGCTGTTTC TGCTCAGGAA GCTCAGGCAG CAGCCATCTT GTCTCAGGCC 
AGGATGGCTC TTAAAGGACC TCCTGGTCCA ATGGGATTCA CTGGGCGTCC CGGACCCTTG 
GGAAATCCTG GAAGTCCTGG TCTGAAAGGA GAGAGTGGAG ACCCTGGACC CCAGGGCCCG 
AGGGGATCTC TAGGCTTGAT GGGACCTCCA GGCAAATCAG GAAGAAGAGG TCGCGCTGGA 
GCCGACGGTG CCAGAGGAAT GCCTGGAGAG TCTGGTTCTA AGGGTGATCG TGGTTTTGAT 
GGTCTTCCTG GTTTACCTGG AGACAAAGGA CACAGGGGTG ACTCGGGACC AATGGGACCT 
CCAGGATCTC AGGGAGAGGA TGGAGAAAGG GGAGACGACG GAGACGTTGG ACCCCGGGGT 
CTTCCAGGTG AACCAGGACC TCGTGGTTTG CTTGGACCTA AAGGTCCTCC TGGAATCCCA 
GGCCCTCCTG GTGTACGAGG AAGTGACGGA CCCCACGGTC CAAAAGGAAA CTTGGGTCCT 
CAAGGTGAGC CAGGACCCCC TGGTCAGCAG GGAACCCCAG GAACTCAGGG AATGCCAGGA 
CCTCAAGGAC ATACTGGACC TCCAGGAGAA AAGGGTCCAA CAGGAAAACC GGGTTTACCA 
GGAATGCCTG GAGCTGATGG CCCTCCTGGT CACCCAGGAA AGGAAGGCCC ATCAGGCACC 
AAAGGAAACC AGGGTCCCAG TGGTCCCCAG GGGCCTATTG GCTATCCTGG TCCTCGTGGC 
CTCAAGGGCG GTCAAGGAAT CCGGGGATTG AAGGGTCACA AAGGAGAGAA GGGAGAAGAT 
GGTTTCCCTG GAATTAAGGG AGACTTTGGG ATCAAGGGTG AGAGGGGTGA GATTGGAGTA 
CCAGGTCCTA GAGGAGAGGA CGGTCCAGAG GGGCCAAAAG GTCGTTTTGG TCCCCCTGGT 
GAAACTGGAC CACTCGGAGC AGTAGGAGAG AAGGGTAAAC TTGGTGTACC GGGACTTCCT 
GGATATCCTG GAAGACAAGG ACCAAAGGGA TCTCTTGGAT TCCCAGGATT CCCTGGATCC 
AATGGCGAGA AGGGAACAAG GGGTCTCACT GGCAAATCAG GACCAAGAGG ACAAAGAGGA 
CCAACGGGCC CCAGAGGGCA GAGGGGACCG AGGGGCGCAA CTGGCAAACT GGGGCCAAAG 
GGGACTTCGG GAAGTGATGG CCCCCCTGGT CCTCCTGGAG AAAGGGGCTT GCCTGGGCCT 
CAGGGAGCCA ATGGGTTCCC CGGACCTAAA GGGCCACCGG GGCCACCAGG GAAGGACGGG 
CTGCCTGGAC ACCCTGGGCA GAGAGGAGAA GTGGGTTTCC AAGGTAAAAT GGGTCCACCT 
GGGCCTCCTG GAGTTGTTGG ACCTCAGGGT CCAGCTGGAG AGACTGGCCC TAATGGTGAA 
CGTGGCCACC CTGGACCACC AGGTCCACCA GGAGAACAGG GACTTCCTGG TCCCTCAGGA 
AAGGAGGGAA CCAAAGGAGA CCCTGGACCC CCAGGAGGCC CTGGCAAAGA TGGGCCTCCT 
GGACTGAGGG GCTTCCCTGG TGAGAGAGGA CTGCCAGGAA CACCTGGAGG TGGAGGACTC 
AAAGGAAGCG AAGGACCTGC TGGACCTCCT GGACCGGCTG GCTCTCCTGG TGAGAGGGGG 
CAATCTGGTA CCGCTGGGCC TGTTGGACCT CCTGGAAGAC CAGGTCCTCA GGGACCTCCT 
GGACCTGCAG GAGAGAAAGG TGTTTCTGGT GAGAAAGGCC CAATTGGCCC AGCTGGTCGT 
GACGGACTAC AGGGTCCTGT GGGTCTGCCT GGCCCAGCTG GACCGCCCGG AGTACCTGGA 
GAAGATGGAG ATAAGGGGGA GGTTGGAGAA CACGGTCAGA AAGGTGCCAA GGGAGGGAAA 
GGAGAGCATG GTCCTCCAGG TCCACCTGGA CCAATGGGAC CAGTCGGCCA GCCTGGTGCT 
CCAGGTGCTG ATGGAGAGCT TGGGCCGAGG GGTCAGCAGG GACCTTTTGG AGCTAAAGGG 
GATGAAGGAA CCAGAGGATT CCCAGGAGCC CCAGGACCCA TTGGACTCCA GGGTTTGCCT 
GGGCCACCAG GTGAGAAGGG AGAAACCGGA GATGTTGGAC CTCTGGGACC TCCAGGCCCT 
CCAGGACCCC GTGGCCCTGC TGGACCCAAC GGTGCTGATG GCCCTCAAGG GCCTCCTGGT 
GGTTTGGGTA ATCCTGGACC TATCGGTGAG AAGGGAGAGC CTGGAGAGTC TGGACCCCCT 
GGAATTGGAG GGGAGCCTGG AAAGAAGGGC CCCAGAGGGG AACGTGGGGA GAAGGGGGAG 
GCTGGACAAC CAGGAACCGC TGGATCCACT GGAGGACGTG GACTACCTGG AGATGATGGA 
CCCAAAGGAA ACCCAGGTCC TGTTGGTTTC CCCGGTGATC CTGGTCCACC GGGTGAGGTT 
GGACCCAGAG GTCAAGACGG TGCCAAGGGA GAAAGAGGAG AAGATGGTGA AGCAGGAGAG 
TCTGGATCCC CTGGACCTCC CGGTGAGAAC GGACCCCCCG GCCCCCTAGG AAAGAGGGGT 
CCTGCTGGAA CAAGAGGACC AGAGGGACGC CAGGGAGAGA AGGGAACAAA GGGGGATCCA 
GGTGCTGTTG GCCCCCCAGG CAAAACAGGC CCTGTGGGTC CACAGGGAGT GCCAGGAAAA 
CCTGGAACAG AAGGCCTTAG AGGGCTTCCA GGATCAGTGG GTGAGCAAGG ATCTCCAGGA 
CCTGCAGGAC AGAAAGGACC ACCTGGTCCT ATTGGACCTC CCGGTTTACC TGGTCTGCGT 
GGAGATTCTG GCGGCAAAGG AGAAAAGGGA CACCCGGGTC TTATCGGTCT CATTGGACCC 
CCAGGAGAAC AGGGTGAGAA AGGAGACAGA GGCATGCCTG GGCCCCATGG ATCCAATGGA 
CCAAAAGGAG AGACTGGAAT GCCTGGAGGC ACAGGACCTA TCGGCCCCTC TGGTCCTCCT 
GGTCTGCCTG GTCCTCAAGG TATTAAAGGA GCCAAAGGTT CTTCTGGTGG AGCTGGTCCA 
AAAGGAGAAA AAGGAGTACA AGGACCCCCT GGACTTCCTG GCCCACCAGG GGAGGTTATT 
CAGCCACTAC CCATCCAAAG GAGTCCCAAG TCCAAGCGGT CCATTGATGC CAGCCAGCTA 
CTTCCTGAGA ATGACATGCC TGCATCCGAT GCCACCGGGA CAGAATTCCT GATGGGTAGT 
GAAGGCATGG AGGAGATCTT TGGCTCCCTC AACTCGCTTC GGCAGGAGAT TGAGACCATG 
CGCTTCCCTC TGGGCACCCA GGACAGCCCC GGCCGCACCT GCCAAGACCT GCACCTCAGT 
CAGCCAGAAC TCAAAGATGG AGAATACTGG ATTGACCCCA ACCAGGGCTG CTCCAGAGAC 
GCCTTCAAGG TCTTCTGTAA CTTTACTAAG GGGGCAGAGA CGTGCCTCTA CCCCAGCCAG 
AGCATCAGCA CGGTGAAGAT GAATTCCTGG AACAAAGAGA CTCCAGGTTC CTGGTACAGT 
CAGTTCTCCA CTGGCAGTAA GTTTTCATAC GTGGACTCCA ACGGCGAACC TGTGGGTGTG 
GTTCAGTTGG GTTTCCTTCG TCTCTTGAGC GTGCAGGCCC ACCAGACTCT CACATACCAC 
TGCCACCGCT CGGTAGCGTG GGCTGACCGC AACGCCAAGA ACACATACAG CCGAGCTCTG 
CACTTCAGGG GTGCCAACGA CGAGGATCTA AGCTATGAGA CCAATCCATA CGTCAAGGCT 
TTGGTTGATG GCTGCTCTTA TCGTAAGGGC TTTGACCGGA CAGTCCTGGA GATCAACACA 
CCACAGGTGG AACATCTTCC TCTGCTGGAT ATCAAGGTGT CAGACTTTGG GGAGAGCAAC 
CAGCAGTTTG GCTTTGAAGT GGGACCTGTC TGTTTCCAAG GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101066527 ( XM_011617367.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011615669.1 Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X3, mRNA.

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CloneID	OTo22618
Clone ID Related Accession (Same CDS sequence)	XM_011617367.1
Accession Version	XM_011617367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6129bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	collagen alpha-2(XI) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071261.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGGC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATAAG 
AAGCAGCTCA ATGGAAAAGG AGCCCCCACT AAATCCACTG CTGTTAAAGC AAAACCTACC 
CCAGCAAAGC CAGCCAAGTC TGGATCATAT AAGCTTGTAG TCAAGTCCCC TATGCCCATC 
AAAGCAGCAA AGTCTACAGC CAAGCCATTT AAAGGCAGGC CCACTGCAGT AGAGATCCTT 
CTCTCTAAGA TAAACCCAAT AACACCATCT AAATCCACAA TTTCCTTGAT TAAAAATGGC 
AACGGTAAAT CAGCTGCAGC AAAGACAAAT GGATTGGCCA AAGCCAACAA CAATGGAAAT 
GGAAGAGCAA CAGGCGCTAA AGTAAATGGC AATGGCAAAG CTGATTCTAA GCCCACAGCT 
CAGGCCAGAG TGAGCGGAAA TGGCAAAGTT GTAGTGGAGA AAAAAGTCAT CATAAATGCC 
AAATCTTCAG CAGAAAAGAA ATCCAGCGGA AATGGAAAAG TCACCATTGT AACCAAGAGT 
AATGGTGCAG CAGAGAAGAA AACCAGTGGT AGTGGCAAAG CTACCATTGA GGTTAAAGAA 
ATTAAAGTCA AAGTTCATGG AAAACCTGAA AATAAAGTTG CAGGTGAGGC CAAGGCTAAC 
GGCAATGGTA AAATTATCAC TGTGGTAGAA AAGAAATTCA CCAATGGTGC TGCGAATCGG 
GCAAAGATTG AAACCACTAC AAGAGCAAAG ACGGAGAAAG TTATCAAAGT TGAAAAGATA 
GAAAAGACTA CGCTCAGTAC AGCTAAATCA GCCACAAAAC TAGATGCTTC TAAAGCAGCC 
AAACCCACAA AAGCCACAAA ATCTGATGCT ACAACATTAA CAAAGGCCAC CAAAGCAGCG 
GCAGCAGCAG GAAAGACCCA AGCAAGGGCA GAGGTCAAAG AAGTCACCCA AGTTAAAACT 
GTTGTCACCA AAGTCCCTCT GGTCAAAACC ACTGTGAGCA AACCAGCGAA GAATGTGGTG 
GAGAGAGTTA CGATGCCCAG GAAAGTAGCC TCGACGTCCA TGCCCGTGAG GCCCACTCCA 
CCCAGACCTA CGAAACCCAA GATGGCGGTG GACATTAATG TCAAATCCCA GCACAAACCT 
TTCCCACCAT TTGGCAAGAC TGCGTTGAGT GGAACTACGA AGAAAGTTAC CAACGGTTAT 
CAGCAGGCTG GGGTGAGAGG CCAGAAAGGA GAGAAAGGAG AACCTGCTGT TTTGGAGCCT 
GGGATGCTGA TTGAAGGACC ACCAGGACCT GAGGGACCTG CTGGCCTACC TGGTCCTTCT 
GGCCCTTCTG GACCTCCTGG CTCTGTGGGT GACCCAGGAG AGAGGGGCCC TCCTGGTAAG 
GCTGGTCTTC CTGGAGCTGA TGGAGTCCCT GGACCTCCTG GGACCTCTGT TATGCTGCCT 
TTCCGTTTTG GTCAGAGTGG AGGAGATAAA GGTCCTGCTG TTTCTGCTCA GGAAGCTCAG 
GCAGCAGCCA TCTTGTCTCA GGCCAGGATG GCTCTTAAAG GACCTCCTGG TCCAATGGGA 
TTCACTGGGC GTCCCGGACC CTTGGGAAAT CCTGGAAGTC CTGGTCTGAA AGGAGAGAGT 
GGAGACCCTG GACCCCAGGG CCCGAGGGGA TCTCTAGGCT TGATGGGACC TCCAGGCAAA 
TCAGGAAGAA GAGGTCGCGC TGGAGCCGAC GGTGCCAGAG GAATGCCTGG AGAGTCTGGT 
TCTAAGGGTG ATCGTGGTTT TGATGGTCTT CCTGGTTTAC CTGGAGACAA AGGACACAGG 
GGTGACTCGG GACCAATGGG ACCTCCAGGA TCTCAGGGAG AGGATGGAGA AAGGGGAGAC 
GACGGAGACG TTGGACCCCG GGGTCTTCCA GGTGAACCAG GACCTCGTGG TTTGCTTGGA 
CCTAAAGGTC CTCCTGGAAT CCCAGGCCCT CCTGGTGTAC GAGGAAGTGA CGGACCCCAC 
GGTCCAAAAG GAAACTTGGG TCCTCAAGGT GAGCCAGGAC CCCCTGGTCA GCAGGGAACC 
CCAGGAACTC AGGGAATGCC AGGACCTCAA GGACATACTG GACCTCCAGG AGAAAAGGGT 
CCAACAGGAA AACCGGGTTT ACCAGGAATG CCTGGAGCTG ATGGCCCTCC TGGTCACCCA 
GGAAAGGAAG GCCCATCAGG CACCAAAGGA AACCAGGGTC CCAGTGGTCC CCAGGGGCCT 
ATTGGCTATC CTGGTCCTCG TGGCCTCAAG GGCGGTCAAG GAATCCGGGG ATTGAAGGGT 
CACAAAGGAG AGAAGGGAGA AGATGGTTTC CCTGGAATTA AGGGAGACTT TGGGATCAAG 
GGTGAGAGGG GTGAGATTGG AGTACCAGGT CCTAGAGGAG AGGACGGTCC AGAGGGGCCA 
AAAGGTCGTT TTGGTCCCCC TGGTGAAACT GGACCACTCG GAGCAGTAGG AGAGAAGGGT 
AAACTTGGTG TACCGGGACT TCCTGGATAT CCTGGAAGAC AAGGACCAAA GGGATCTCTT 
GGATTCCCAG GATTCCCTGG ATCCAATGGC GAGAAGGGAA CAAGGGGTCT CACTGGCAAA 
TCAGGACCAA GAGGACAAAG AGGACCAACG GGCCCCAGAG GGCAGAGGGG ACCGAGGGGC 
GCAACTGGCA AACTGGGGCC AAAGGGGACT TCGGGAAGTG ATGGCCCCCC TGGTCCTCCT 
GGAGAAAGGG GCTTGCCTGG GCCTCAGGGA GCCAATGGGT TCCCCGGACC TAAAGGGCCA 
CCGGGGCCAC CAGGGAAGGA CGGGCTGCCT GGACACCCTG GGCAGAGAGG AGAAGTGGGT 
TTCCAAGGTA AAATGGGTCC ACCTGGGCCT CCTGGAGTTG TTGGACCTCA GGGTCCAGCT 
GGAGAGACTG GCCCTAATGG TGAACGTGGC CACCCTGGAC CACCAGGTCC ACCAGGAGAA 
CAGGGACTTC CTGGTCCCTC AGGAAAGGAG GGAACCAAAG GAGACCCTGG ACCCCCAGGA 
GGCCCTGGCA AAGATGGGCC TCCTGGACTG AGGGGCTTCC CTGGTGAGAG AGGACTGCCA 
GGAACACCTG GAGGTGGAGG ACTCAAAGGA AGCGAAGGAC CTGCTGGACC TCCTGGACCG 
GCTGGCTCTC CTGGTGAGAG GGGGCAATCT GGTACCGCTG GGCCTGTTGG ACCTCCTGGA 
AGACCAGGTC CTCAGGGACC TCCTGGACCT GCAGGAGAGA AAGGTGTTTC TGGTGAGAAA 
GGCCCAATTG GCCCAGCTGG TCGTGACGGA CTACAGGGTC CTGTGGGTCT GCCTGGCCCA 
GCTGGACCGC CCGGAGTACC TGGAGAAGAT GGAGATAAGG GGGAGGTTGG AGAACACGGT 
CAGAAAGGTG CCAAGGGAGG GAAAGGAGAG CATGGTCCTC CAGGTCCACC TGGACCAATG 
GGACCAGTCG GCCAGCCTGG TGCTCCAGGT GCTGATGGAG AGCTTGGGCC GAGGGGTCAG 
CAGGGACCTT TTGGAGCTAA AGGGGATGAA GGAACCAGAG GATTCCCAGG AGCCCCAGGA 
CCCATTGGAC TCCAGGGTTT GCCTGGGCCA CCAGGTGAGA AGGGAGAAAC CGGAGATGTT 
GGACCTCTGG GACCTCCAGG CCCTCCAGGA CCCCGTGGCC CTGCTGGACC CAACGGTGCT 
GATGGCCCTC AAGGGCCTCC TGGTGGTTTG GGTAATCCTG GACCTATCGG TGAGAAGGGA 
GAGCCTGGAG AGTCTGGACC CCCTGGAATT GGAGGGGAGC CTGGAAAGAA GGGCCCCAGA 
GGGGAACGTG GGGAGAAGGG GGAGGCTGGA CAACCAGGAA CCGCTGGATC CACTGGAGGA 
CGTGGACTAC CTGGAGATGA TGGACCCAAA GGAAACCCAG GTCCTGTTGG TTTCCCCGGT 
GATCCTGGTC CACCGGGTGA GGTTGGACCC AGAGGTCAAG ACGGTGCCAA GGGAGAAAGA 
GGAGAAGATG GTGAAGCAGG AGAGTCTGGA TCCCCTGGAC CTCCCGGTGA GAACGGACCC 
CCCGGCCCCC TAGGAAAGAG GGGTCCTGCT GGAACAAGAG GACCAGAGGG ACGCCAGGGA 
GAGAAGGGAA CAAAGGGGGA TCCAGGTGCT GTTGGCCCCC CAGGCAAAAC AGGCCCTGTG 
GGTCCACAGG GAGTGCCAGG AAAACCTGGA ACAGAAGGCC TTAGAGGGCT TCCAGGATCA 
GTGGGTGAGC AAGGATCTCC AGGACCTGCA GGACAGAAAG GACCACCTGG TCCTATTGGA 
CCTCCCGGTT TACCTGGTCT GCGTGGAGAT TCTGGCGGCA AAGGAGAAAA GGGACACCCG 
GGTCTTATCG GTCTCATTGG ACCCCCAGGA GAACAGGGTG AGAAAGGAGA CAGAGGCATG 
CCTGGGCCCC ATGGATCCAA TGGACCAAAA GGAGAGACTG GAATGCCTGG AGGCACAGGA 
CCTATCGGCC CCTCTGGTCC TCCTGGTCTG CCTGGTCCTC AAGGTATTAA AGGAGCCAAA 
GGTTCTTCTG GTGGAGCTGG TCCAAAAGGA GAAAAAGGAG TACAAGGACC CCCTGGACTT 
CCTGGCCCAC CAGGGGAGGT TATTCAGCCA CTACCCATCC AAAGGAGTCC CAAGTCCAAG 
CGGTCCATTG ATGCCAGCCA GCTACTTCCT GAGAATGACA TGCCTGCATC CGATGCCACC 
GGGACAGAAT TCCTGATGGG TAGTGAAGGC ATGGAGGAGA TCTTTGGCTC CCTCAACTCG 
CTTCGGCAGG AGATTGAGAC CATGCGCTTC CCTCTGGGCA CCCAGGACAG CCCCGGCCGC 
ACCTGCCAAG ACCTGCACCT CAGTCAGCCA GAACTCAAAG ATGGAGAATA CTGGATTGAC 
CCCAACCAGG GCTGCTCCAG AGACGCCTTC AAGGTCTTCT GTAACTTTAC TAAGGGGGCA 
GAGACGTGCC TCTACCCCAG CCAGAGCATC AGCACGGTGA AGATGAATTC CTGGAACAAA 
GAGACTCCAG GTTCCTGGTA CAGTCAGTTC TCCACTGGCA GTAAGTTTTC ATACGTGGAC 
TCCAACGGCG AACCTGTGGG TGTGGTTCAG TTGGGTTTCC TTCGTCTCTT GAGCGTGCAG 
GCCCACCAGA CTCTCACATA CCACTGCCAC CGCTCGGTAG CGTGGGCTGA CCGCAACGCC 
AAGAACACAT ACAGCCGAGC TCTGCACTTC AGGGGTGCCA ACGACGAGGA TCTAAGCTAT 
GAGACCAATC CATACGTCAA GGCTTTGGTT GATGGCTGCT CTTATCGTAA GGGCTTTGAC 
CGGACAGTCC TGGAGATCAA CACACCACAG GTGGAACATC TTCCTCTGCT GGATATCAAG 
GTGTCAGACT TTGGGGAGAG CAACCAGCAG TTTGGCTTTG AAGTGGGACC TGTCTGTTTC 
CAAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615669.1
CDS	392..6520
Translation	MDTGDCPCRKKRRPWRMDFSSFALVTVMLIVCQTALVRADPVDVLRALQVPSLPEGVKKVPGFCTSRRSSSPDHAYRITKKAQISAPTKQLFSGRFPENFSIMALVKAQAGLQAFLLSIYSEQGIQQLGIEMGRSPVFLYEDQNGKPAPEDYPLFRGVNLADGKWHRIALSVSKKNVTLLLDCKKKMTRPLPRSNSAEVDTNGITVFGARLLDEEVFQGDIQQLLIASNPQAAYDFCEHYSPDCDSPLPKTQAQDPNTYKKQLNGKGAPTKSTAVKAKPTPAKPAKSGSYKLVVKSPMPIKAAKSTAKPFKGRPTAVEILLSKINPITPSKSTISLIKNGNGKSAAAKTNGLAKANNNGNGRATGAKVNGNGKADSKPTAQARVSGNGKVVVEKKVIINAKSSAEKKSSGNGKVTIVTKSNGAAEKKTSGSGKATIEVKEIKVKVHGKPENKVAGEAKANGNGKIITVVEKKFTNGAANRAKIETTTRAKTEKVIKVEKIEKTTLSTAKSATKLDASKAAKPTKATKSDATTLTKATKAAAAAGKTQARAEVKEVTQVKTVVTKVPLVKTTVSKPAKNVVERVTMPRKVASTSMPVRPTPPRPTKPKMAVDINVKSQHKPFPPFGKTALSGTTKKVTNGYQQAGVRGQKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPSGPPGSVGDPGERGPPGKAGLPGADGVPGPPGTSVMLPFRFGQSGGDKGPAVSAQEAQAAAILSQARMALKGPPGPMGFTGRPGPLGNPGSPGLKGESGDPGPQGPRGSLGLMGPPGKSGRRGRAGADGARGMPGESGSKGDRGFDGLPGLPGDKGHRGDSGPMGPPGSQGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGIPGPPGVRGSDGPHGPKGNLGPQGEPGPPGQQGTPGTQGMPGPQGHTGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGPIGYPGPRGLKGGQGIRGLKGHKGEKGEDGFPGIKGDFGIKGERGEIGVPGPRGEDGPEGPKGRFGPPGETGPLGAVGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGSNGEKGTRGLTGKSGPRGQRGPTGPRGQRGPRGATGKLGPKGTSGSDGPPGPPGERGLPGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKMGPPGPPGVVGPQGPAGETGPNGERGHPGPPGPPGEQGLPGPSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGGGGLKGSEGPAGPPGPAGSPGERGQSGTAGPVGPPGRPGPQGPPGPAGEKGVSGEKGPIGPAGRDGLQGPVGLPGPAGPPGVPGEDGDKGEVGEHGQKGAKGGKGEHGPPGPPGPMGPVGQPGAPGADGELGPRGQQGPFGAKGDEGTRGFPGAPGPIGLQGLPGPPGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGIGGEPGKKGPRGERGEKGEAGQPGTAGSTGGRGLPGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGERGEDGEAGESGSPGPPGENGPPGPLGKRGPAGTRGPEGRQGEKGTKGDPGAVGPPGKTGPVGPQGVPGKPGTEGLRGLPGSVGEQGSPGPAGQKGPPGPIGPPGLPGLRGDSGGKGEKGHPGLIGLIGPPGEQGEKGDRGMPGPHGSNGPKGETGMPGGTGPIGPSGPPGLPGPQGIKGAKGSSGGAGPKGEKGVQGPPGLPGPPGEVIQPLPIQRSPKSKRSIDASQLLPENDMPASDATGTEFLMGSEGMEEIFGSLNSLRQEIETMRFPLGTQDSPGRTCQDLHLSQPELKDGEYWIDPNQGCSRDAFKVFCNFTKGAETCLYPSQSISTVKMNSWNKETPGSWYSQFSTGSKFSYVDSNGEPVGVVQLGFLRLLSVQAHQTLTYHCHRSVAWADRNAKNTYSRALHFRGANDEDLSYETNPYVKALVDGCSYRKGFDRTVLEINTPQVEHLPLLDIKVSDFGESNQQFGFEVGPVCFQG

Target ORF information:

RefSeq Version	XM_011617367.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617367.1

ORF Insert Sequence:

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGGC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATAAG 
AAGCAGCTCA ATGGAAAAGG AGCCCCCACT AAATCCACTG CTGTTAAAGC AAAACCTACC 
CCAGCAAAGC CAGCCAAGTC TGGATCATAT AAGCTTGTAG TCAAGTCCCC TATGCCCATC 
AAAGCAGCAA AGTCTACAGC CAAGCCATTT AAAGGCAGGC CCACTGCAGT AGAGATCCTT 
CTCTCTAAGA TAAACCCAAT AACACCATCT AAATCCACAA TTTCCTTGAT TAAAAATGGC 
AACGGTAAAT CAGCTGCAGC AAAGACAAAT GGATTGGCCA AAGCCAACAA CAATGGAAAT 
GGAAGAGCAA CAGGCGCTAA AGTAAATGGC AATGGCAAAG CTGATTCTAA GCCCACAGCT 
CAGGCCAGAG TGAGCGGAAA TGGCAAAGTT GTAGTGGAGA AAAAAGTCAT CATAAATGCC 
AAATCTTCAG CAGAAAAGAA ATCCAGCGGA AATGGAAAAG TCACCATTGT AACCAAGAGT 
AATGGTGCAG CAGAGAAGAA AACCAGTGGT AGTGGCAAAG CTACCATTGA GGTTAAAGAA 
ATTAAAGTCA AAGTTCATGG AAAACCTGAA AATAAAGTTG CAGGTGAGGC CAAGGCTAAC 
GGCAATGGTA AAATTATCAC TGTGGTAGAA AAGAAATTCA CCAATGGTGC TGCGAATCGG 
GCAAAGATTG AAACCACTAC AAGAGCAAAG ACGGAGAAAG TTATCAAAGT TGAAAAGATA 
GAAAAGACTA CGCTCAGTAC AGCTAAATCA GCCACAAAAC TAGATGCTTC TAAAGCAGCC 
AAACCCACAA AAGCCACAAA ATCTGATGCT ACAACATTAA CAAAGGCCAC CAAAGCAGCG 
GCAGCAGCAG GAAAGACCCA AGCAAGGGCA GAGGTCAAAG AAGTCACCCA AGTTAAAACT 
GTTGTCACCA AAGTCCCTCT GGTCAAAACC ACTGTGAGCA AACCAGCGAA GAATGTGGTG 
GAGAGAGTTA CGATGCCCAG GAAAGTAGCC TCGACGTCCA TGCCCGTGAG GCCCACTCCA 
CCCAGACCTA CGAAACCCAA GATGGCGGTG GACATTAATG TCAAATCCCA GCACAAACCT 
TTCCCACCAT TTGGCAAGAC TGCGTTGAGT GGAACTACGA AGAAAGTTAC CAACGGTTAT 
CAGCAGGCTG GGGTGAGAGG CCAGAAAGGA GAGAAAGGAG AACCTGCTGT TTTGGAGCCT 
GGGATGCTGA TTGAAGGACC ACCAGGACCT GAGGGACCTG CTGGCCTACC TGGTCCTTCT 
GGCCCTTCTG GACCTCCTGG CTCTGTGGGT GACCCAGGAG AGAGGGGCCC TCCTGGTAAG 
GCTGGTCTTC CTGGAGCTGA TGGAGTCCCT GGACCTCCTG GGACCTCTGT TATGCTGCCT 
TTCCGTTTTG GTCAGAGTGG AGGAGATAAA GGTCCTGCTG TTTCTGCTCA GGAAGCTCAG 
GCAGCAGCCA TCTTGTCTCA GGCCAGGATG GCTCTTAAAG GACCTCCTGG TCCAATGGGA 
TTCACTGGGC GTCCCGGACC CTTGGGAAAT CCTGGAAGTC CTGGTCTGAA AGGAGAGAGT 
GGAGACCCTG GACCCCAGGG CCCGAGGGGA TCTCTAGGCT TGATGGGACC TCCAGGCAAA 
TCAGGAAGAA GAGGTCGCGC TGGAGCCGAC GGTGCCAGAG GAATGCCTGG AGAGTCTGGT 
TCTAAGGGTG ATCGTGGTTT TGATGGTCTT CCTGGTTTAC CTGGAGACAA AGGACACAGG 
GGTGACTCGG GACCAATGGG ACCTCCAGGA TCTCAGGGAG AGGATGGAGA AAGGGGAGAC 
GACGGAGACG TTGGACCCCG GGGTCTTCCA GGTGAACCAG GACCTCGTGG TTTGCTTGGA 
CCTAAAGGTC CTCCTGGAAT CCCAGGCCCT CCTGGTGTAC GAGGAAGTGA CGGACCCCAC 
GGTCCAAAAG GAAACTTGGG TCCTCAAGGT GAGCCAGGAC CCCCTGGTCA GCAGGGAACC 
CCAGGAACTC AGGGAATGCC AGGACCTCAA GGACATACTG GACCTCCAGG AGAAAAGGGT 
CCAACAGGAA AACCGGGTTT ACCAGGAATG CCTGGAGCTG ATGGCCCTCC TGGTCACCCA 
GGAAAGGAAG GCCCATCAGG CACCAAAGGA AACCAGGGTC CCAGTGGTCC CCAGGGGCCT 
ATTGGCTATC CTGGTCCTCG TGGCCTCAAG GGCGGTCAAG GAATCCGGGG ATTGAAGGGT 
CACAAAGGAG AGAAGGGAGA AGATGGTTTC CCTGGAATTA AGGGAGACTT TGGGATCAAG 
GGTGAGAGGG GTGAGATTGG AGTACCAGGT CCTAGAGGAG AGGACGGTCC AGAGGGGCCA 
AAAGGTCGTT TTGGTCCCCC TGGTGAAACT GGACCACTCG GAGCAGTAGG AGAGAAGGGT 
AAACTTGGTG TACCGGGACT TCCTGGATAT CCTGGAAGAC AAGGACCAAA GGGATCTCTT 
GGATTCCCAG GATTCCCTGG ATCCAATGGC GAGAAGGGAA CAAGGGGTCT CACTGGCAAA 
TCAGGACCAA GAGGACAAAG AGGACCAACG GGCCCCAGAG GGCAGAGGGG ACCGAGGGGC 
GCAACTGGCA AACTGGGGCC AAAGGGGACT TCGGGAAGTG ATGGCCCCCC TGGTCCTCCT 
GGAGAAAGGG GCTTGCCTGG GCCTCAGGGA GCCAATGGGT TCCCCGGACC TAAAGGGCCA 
CCGGGGCCAC CAGGGAAGGA CGGGCTGCCT GGACACCCTG GGCAGAGAGG AGAAGTGGGT 
TTCCAAGGTA AAATGGGTCC ACCTGGGCCT CCTGGAGTTG TTGGACCTCA GGGTCCAGCT 
GGAGAGACTG GCCCTAATGG TGAACGTGGC CACCCTGGAC CACCAGGTCC ACCAGGAGAA 
CAGGGACTTC CTGGTCCCTC AGGAAAGGAG GGAACCAAAG GAGACCCTGG ACCCCCAGGA 
GGCCCTGGCA AAGATGGGCC TCCTGGACTG AGGGGCTTCC CTGGTGAGAG AGGACTGCCA 
GGAACACCTG GAGGTGGAGG ACTCAAAGGA AGCGAAGGAC CTGCTGGACC TCCTGGACCG 
GCTGGCTCTC CTGGTGAGAG GGGGCAATCT GGTACCGCTG GGCCTGTTGG ACCTCCTGGA 
AGACCAGGTC CTCAGGGACC TCCTGGACCT GCAGGAGAGA AAGGTGTTTC TGGTGAGAAA 
GGCCCAATTG GCCCAGCTGG TCGTGACGGA CTACAGGGTC CTGTGGGTCT GCCTGGCCCA 
GCTGGACCGC CCGGAGTACC TGGAGAAGAT GGAGATAAGG GGGAGGTTGG AGAACACGGT 
CAGAAAGGTG CCAAGGGAGG GAAAGGAGAG CATGGTCCTC CAGGTCCACC TGGACCAATG 
GGACCAGTCG GCCAGCCTGG TGCTCCAGGT GCTGATGGAG AGCTTGGGCC GAGGGGTCAG 
CAGGGACCTT TTGGAGCTAA AGGGGATGAA GGAACCAGAG GATTCCCAGG AGCCCCAGGA 
CCCATTGGAC TCCAGGGTTT GCCTGGGCCA CCAGGTGAGA AGGGAGAAAC CGGAGATGTT 
GGACCTCTGG GACCTCCAGG CCCTCCAGGA CCCCGTGGCC CTGCTGGACC CAACGGTGCT 
GATGGCCCTC AAGGGCCTCC TGGTGGTTTG GGTAATCCTG GACCTATCGG TGAGAAGGGA 
GAGCCTGGAG AGTCTGGACC CCCTGGAATT GGAGGGGAGC CTGGAAAGAA GGGCCCCAGA 
GGGGAACGTG GGGAGAAGGG GGAGGCTGGA CAACCAGGAA CCGCTGGATC CACTGGAGGA 
CGTGGACTAC CTGGAGATGA TGGACCCAAA GGAAACCCAG GTCCTGTTGG TTTCCCCGGT 
GATCCTGGTC CACCGGGTGA GGTTGGACCC AGAGGTCAAG ACGGTGCCAA GGGAGAAAGA 
GGAGAAGATG GTGAAGCAGG AGAGTCTGGA TCCCCTGGAC CTCCCGGTGA GAACGGACCC 
CCCGGCCCCC TAGGAAAGAG GGGTCCTGCT GGAACAAGAG GACCAGAGGG ACGCCAGGGA 
GAGAAGGGAA CAAAGGGGGA TCCAGGTGCT GTTGGCCCCC CAGGCAAAAC AGGCCCTGTG 
GGTCCACAGG GAGTGCCAGG AAAACCTGGA ACAGAAGGCC TTAGAGGGCT TCCAGGATCA 
GTGGGTGAGC AAGGATCTCC AGGACCTGCA GGACAGAAAG GACCACCTGG TCCTATTGGA 
CCTCCCGGTT TACCTGGTCT GCGTGGAGAT TCTGGCGGCA AAGGAGAAAA GGGACACCCG 
GGTCTTATCG GTCTCATTGG ACCCCCAGGA GAACAGGGTG AGAAAGGAGA CAGAGGCATG 
CCTGGGCCCC ATGGATCCAA TGGACCAAAA GGAGAGACTG GAATGCCTGG AGGCACAGGA 
CCTATCGGCC CCTCTGGTCC TCCTGGTCTG CCTGGTCCTC AAGGTATTAA AGGAGCCAAA 
GGTTCTTCTG GTGGAGCTGG TCCAAAAGGA GAAAAAGGAG TACAAGGACC CCCTGGACTT 
CCTGGCCCAC CAGGGGAGGT TATTCAGCCA CTACCCATCC AAAGGAGTCC CAAGTCCAAG 
CGGTCCATTG ATGCCAGCCA GCTACTTCCT GAGAATGACA TGCCTGCATC CGATGCCACC 
GGGACAGAAT TCCTGATGGG TAGTGAAGGC ATGGAGGAGA TCTTTGGCTC CCTCAACTCG 
CTTCGGCAGG AGATTGAGAC CATGCGCTTC CCTCTGGGCA CCCAGGACAG CCCCGGCCGC 
ACCTGCCAAG ACCTGCACCT CAGTCAGCCA GAACTCAAAG ATGGAGAATA CTGGATTGAC 
CCCAACCAGG GCTGCTCCAG AGACGCCTTC AAGGTCTTCT GTAACTTTAC TAAGGGGGCA 
GAGACGTGCC TCTACCCCAG CCAGAGCATC AGCACGGTGA AGATGAATTC CTGGAACAAA 
GAGACTCCAG GTTCCTGGTA CAGTCAGTTC TCCACTGGCA GTAAGTTTTC ATACGTGGAC 
TCCAACGGCG AACCTGTGGG TGTGGTTCAG TTGGGTTTCC TTCGTCTCTT GAGCGTGCAG 
GCCCACCAGA CTCTCACATA CCACTGCCAC CGCTCGGTAG CGTGGGCTGA CCGCAACGCC 
AAGAACACAT ACAGCCGAGC TCTGCACTTC AGGGGTGCCA ACGACGAGGA TCTAAGCTAT 
GAGACCAATC CATACGTCAA GGCTTTGGTT GATGGCTGCT CTTATCGTAA GGGCTTTGAC 
CGGACAGTCC TGGAGATCAA CACACCACAG GTGGAACATC TTCCTCTGCT GGATATCAAG 
GTGTCAGACT TTGGGGAGAG CAACCAGCAG TTTGGCTTTG AAGTGGGACC TGTCTGTTTC 
CAAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101066527 ( XM_011617369.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011615671.1 Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X4, mRNA.

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CloneID	OTo22619
Clone ID Related Accession (Same CDS sequence)	XM_011617369.1
Accession Version	XM_011617369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	collagen alpha-2(XI) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071261.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGGC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATTAC 
TTTGATGAGG AGCAGGATGA CCATGAATAC CCTTATTATT ATGAGGAAAC AACAGGCCGT 
CCCTCCTCCT CTGCACCCCC CTCTCCCCAA CCTGGGGCCC CCAGTCAACA GGATGTAGAC 
ACTGAATATT CCGAGGCTGG CCACACCCCT ACAGAGAGAG ATTATTACTA TGAGGAGACG 
GTACCACTGC CAGAGGGTGA GGTGGTTGGG CAAGAGGAAC TCGCCGTACA GCCCCAGGTG 
GAGCCTGTGT TGGAGGGAGA AGAGGTGCAT TCGACCAAGG CTGTCGGTGA GGGAGAGGAG 
GCCTTCACAG AGGAGTATGT GACTGGAGAT GTGACCCTTA AGGAATACGA CTATTCCTAC 
AGGGACTACA ATGAGCCATT AGTGGAGACT GGAGAAGTCG ATGCCAACAT GGGCCCCGCC 
CTCTCTGCTG TGACAGACGA GGGAGGCGCT GGGGTGAGAG GCCAGAAAGG AGAGAAAGGA 
GAACCTGCTG TTTTGGAGCC TGGGATGCTG ATTGAAGGAC CACCAGGACC TGAGGGACCT 
GCTGGCCTAC CTGGTCCTTC TGGCCCTTCT GGACCTCCTG GCTCTGTGGG TGACCCAGGA 
GAGAGGGGCC CTCCTGGTAA GGCTGGTCTT CCTGGAGCTG ATGGAGTCCC TGGACCTCCT 
GGGACCTCTG TTATGCTGCC TTTCCGTTTT GGTCAGAGTG GAGGAGATAA AGGTCCTGCT 
GTTTCTGCTC AGGAAGCTCA GGCAGCAGCC ATCTTGTCTC AGGCCAGGAT GGCTCTTAAA 
GGACCTCCTG GTCCAATGGG ATTCACTGGG CGTCCCGGAC CCTTGGGAAA TCCTGGAAGT 
CCTGGTCTGA AAGGAGAGAG TGGAGACCCT GGACCCCAGG GCCCGAGGGG ATCTCTAGGC 
TTGATGGGAC CTCCAGGCAA ATCAGGAAGA AGAGGTCGCG CTGGAGCCGA CGGTGCCAGA 
GGAATGCCTG GAGAGTCTGG TTCTAAGGGT GATCGTGGTT TTGATGGTCT TCCTGGTTTA 
CCTGGAGACA AAGGACACAG GGGTGACTCG GGACCAATGG GACCTCCAGG ATCTCAGGGA 
GAGGATGGAG AAAGGGGAGA CGACGGAGAC GTTGGACCCC GGGGTCTTCC AGGTGAACCA 
GGACCTCGTG GTTTGCTTGG ACCTAAAGGT CCTCCTGGAA TCCCAGGCCC TCCTGGTGTA 
CGAGGAAGTG ACGGACCCCA CGGTCCAAAA GGAAACTTGG GTCCTCAAGG TGAGCCAGGA 
CCCCCTGGTC AGCAGGGAAC CCCAGGAACT CAGGGAATGC CAGGACCTCA AGGACATACT 
GGACCTCCAG GAGAAAAGGG TCCAACAGGA AAACCGGGTT TACCAGGAAT GCCTGGAGCT 
GATGGCCCTC CTGGTCACCC AGGAAAGGAA GGCCCATCAG GCACCAAAGG AAACCAGGGT 
CCCAGTGGTC CCCAGGGGCC TATTGGCTAT CCTGGTCCTC GTGGCCTCAA GGGCGGTCAA 
GGAATCCGGG GATTGAAGGG TCACAAAGGA GAGAAGGGAG AAGATGGTTT CCCTGGAATT 
AAGGGAGACT TTGGGATCAA GGGTGAGAGG GGTGAGATTG GAGTACCAGG TCCTAGAGGA 
GAGGACGGTC CAGAGGGGCC AAAAGGTCGT TTTGGTCCCC CTGGTGAAAC TGGACCACTC 
GGAGCAGTAG GAGAGAAGGG TAAACTTGGT GTACCGGGAC TTCCTGGATA TCCTGGAAGA 
CAAGGACCAA AGGGATCTCT TGGATTCCCA GGATTCCCTG GATCCAATGG CGAGAAGGGA 
ACAAGGGGTC TCACTGGCAA ATCAGGACCA AGAGGACAAA GAGGACCAAC GGGCCCCAGA 
GGGCAGAGGG GACCGAGGGG CGCAACTGGC AAACTGGGGC CAAAGGGGAC TTCGGGAAGT 
GATGGCCCCC CTGGTCCTCC TGGAGAAAGG GGCTTGCCTG GGCCTCAGGG AGCCAATGGG 
TTCCCCGGAC CTAAAGGGCC ACCGGGGCCA CCAGGGAAGG ACGGGCTGCC TGGACACCCT 
GGGCAGAGAG GAGAAGTGGG TTTCCAAGGT AAAATGGGTC CACCTGGGCC TCCTGGAGTT 
GTTGGACCTC AGGGTCCAGC TGGAGAGACT GGCCCTAATG GTGAACGTGG CCACCCTGGA 
CCACCAGGTC CACCAGGAGA ACAGGGACTT CCTGGTCCCT CAGGAAAGGA GGGAACCAAA 
GGAGACCCTG GACCCCCAGG AGGCCCTGGC AAAGATGGGC CTCCTGGACT GAGGGGCTTC 
CCTGGTGAGA GAGGACTGCC AGGAACACCT GGAGGTGGAG GACTCAAAGG AAGCGAAGGA 
CCTGCTGGAC CTCCTGGACC GGCTGGCTCT CCTGGTGAGA GGGGGCAATC TGGTACCGCT 
GGGCCTGTTG GACCTCCTGG AAGACCAGGT CCTCAGGGAC CTCCTGGACC TGCAGGAGAG 
AAAGGTGTTT CTGGTGAGAA AGGCCCAATT GGCCCAGCTG GTCGTGACGG ACTACAGGGT 
CCTGTGGGTC TGCCTGGCCC AGCTGGACCG CCCGGAGTAC CTGGAGAAGA TGGAGATAAG 
GGGGAGGTTG GAGAACACGG TCAGAAAGGT GCCAAGGGAG GGAAAGGAGA GCATGGTCCT 
CCAGGTCCAC CTGGACCAAT GGGACCAGTC GGCCAGCCTG GTGCTCCAGG TGCTGATGGA 
GAGCTTGGGC CGAGGGGTCA GCAGGGACCT TTTGGAGCTA AAGGGGATGA AGGAACCAGA 
GGATTCCCAG GAGCCCCAGG ACCCATTGGA CTCCAGGGTT TGCCTGGGCC ACCAGGTGAG 
AAGGGAGAAA CCGGAGATGT TGGACCTCTG GGACCTCCAG GCCCTCCAGG ACCCCGTGGC 
CCTGCTGGAC CCAACGGTGC TGATGGCCCT CAAGGGCCTC CTGGTGGTTT GGGTAATCCT 
GGACCTATCG GTGAGAAGGG AGAGCCTGGA GAGTCTGGAC CCCCTGGAAT TGGAGGGGAG 
CCTGGAAAGA AGGGCCCCAG AGGGGAACGT GGGGAGAAGG GGGAGGCTGG ACAACCAGGA 
ACCGCTGGAT CCACTGGAGG ACGTGGACTA CCTGGAGATG ATGGACCCAA AGGAAACCCA 
GGTCCTGTTG GTTTCCCCGG TGATCCTGGT CCACCGGGTG AGGTTGGACC CAGAGGTCAA 
GACGGTGCCA AGGGAGAAAG AGGAGAAGAT GGTGAAGCAG GAGAGTCTGG ATCCCCTGGA 
CCTCCCGGTG AGAACGGACC CCCCGGCCCC CTAGGAAAGA GGGGTCCTGC TGGAACAAGA 
GGACCAGAGG GACGCCAGGG AGAGAAGGGA ACAAAGGGGG ATCCAGGTGC TGTTGGCCCC 
CCAGGCAAAA CAGGCCCTGT GGGTCCACAG GGAGTGCCAG GAAAACCTGG AACAGAAGGC 
CTTAGAGGGC TTCCAGGATC AGTGGGTGAG CAAGGATCTC CAGGACCTGC AGGACAGAAA 
GGACCACCTG GTCCTATTGG ACCTCCCGGT TTACCTGGTC TGCGTGGAGA TTCTGGCGGC 
AAAGGAGAAA AGGGACACCC GGGTCTTATC GGTCTCATTG GACCCCCAGG AGAACAGGGT 
GAGAAAGGAG ACAGAGGCAT GCCTGGGCCC CATGGATCCA ATGGACCAAA AGGAGAGACT 
GGAATGCCTG GAGGCACAGG ACCTATCGGC CCCTCTGGTC CTCCTGGTCT GCCTGGTCCT 
CAAGGTATTA AAGGAGCCAA AGGTTCTTCT GGTGGAGCTG GTCCAAAAGG AGAAAAAGGA 
GTACAAGGAC CCCCTGGACT TCCTGGCCCA CCAGGGGAGG TTATTCAGCC ACTACCCATC 
CAAAGGAGTC CCAAGTCCAA GCGGTCCATT GATGCCAGCC AGCTACTTCC TGAGAATGAC 
ATGCCTGCAT CCGATGCCAC CGGGACAGAA TTCCTGATGG GTAGTGAAGG CATGGAGGAG 
ATCTTTGGCT CCCTCAACTC GCTTCGGCAG GAGATTGAGA CCATGCGCTT CCCTCTGGGC 
ACCCAGGACA GCCCCGGCCG CACCTGCCAA GACCTGCACC TCAGTCAGCC AGAACTCAAA 
GATGGAGAAT ACTGGATTGA CCCCAACCAG GGCTGCTCCA GAGACGCCTT CAAGGTCTTC 
TGTAACTTTA CTAAGGGGGC AGAGACGTGC CTCTACCCCA GCCAGAGCAT CAGCACGGTG 
AAGATGAATT CCTGGAACAA AGAGACTCCA GGTTCCTGGT ACAGTCAGTT CTCCACTGGC 
AGTAAGTTTT CATACGTGGA CTCCAACGGC GAACCTGTGG GTGTGGTTCA GTTGGGTTTC 
CTTCGTCTCT TGAGCGTGCA GGCCCACCAG ACTCTCACAT ACCACTGCCA CCGCTCGGTA 
GCGTGGGCTG ACCGCAACGC CAAGAACACA TACAGCCGAG CTCTGCACTT CAGGGGTGCC 
AACGACGAGG ATCTAAGCTA TGAGACCAAT CCATACGTCA AGGCTTTGGT TGATGGCTGC 
TCTTATCGTA AGGGCTTTGA CCGGACAGTC CTGGAGATCA ACACACCACA GGTGGAACAT 
CTTCCTCTGC TGGATATCAA GGTGTCAGAC TTTGGGGAGA GCAACCAGCA GTTTGGCTTT 
GAAGTGGGAC CTGTCTGTTT CCAAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615671.1
CDS	392..5821
Translation	MDTGDCPCRKKRRPWRMDFSSFALVTVMLIVCQTALVRADPVDVLRALQVPSLPEGVKKVPGFCTSRRSSSPDHAYRITKKAQISAPTKQLFSGRFPENFSIMALVKAQAGLQAFLLSIYSEQGIQQLGIEMGRSPVFLYEDQNGKPAPEDYPLFRGVNLADGKWHRIALSVSKKNVTLLLDCKKKMTRPLPRSNSAEVDTNGITVFGARLLDEEVFQGDIQQLLIASNPQAAYDFCEHYSPDCDSPLPKTQAQDPNTYYFDEEQDDHEYPYYYEETTGRPSSSAPPSPQPGAPSQQDVDTEYSEAGHTPTERDYYYEETVPLPEGEVVGQEELAVQPQVEPVLEGEEVHSTKAVGEGEEAFTEEYVTGDVTLKEYDYSYRDYNEPLVETGEVDANMGPALSAVTDEGGAGVRGQKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPSGPPGSVGDPGERGPPGKAGLPGADGVPGPPGTSVMLPFRFGQSGGDKGPAVSAQEAQAAAILSQARMALKGPPGPMGFTGRPGPLGNPGSPGLKGESGDPGPQGPRGSLGLMGPPGKSGRRGRAGADGARGMPGESGSKGDRGFDGLPGLPGDKGHRGDSGPMGPPGSQGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGIPGPPGVRGSDGPHGPKGNLGPQGEPGPPGQQGTPGTQGMPGPQGHTGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGPIGYPGPRGLKGGQGIRGLKGHKGEKGEDGFPGIKGDFGIKGERGEIGVPGPRGEDGPEGPKGRFGPPGETGPLGAVGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGSNGEKGTRGLTGKSGPRGQRGPTGPRGQRGPRGATGKLGPKGTSGSDGPPGPPGERGLPGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKMGPPGPPGVVGPQGPAGETGPNGERGHPGPPGPPGEQGLPGPSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGGGGLKGSEGPAGPPGPAGSPGERGQSGTAGPVGPPGRPGPQGPPGPAGEKGVSGEKGPIGPAGRDGLQGPVGLPGPAGPPGVPGEDGDKGEVGEHGQKGAKGGKGEHGPPGPPGPMGPVGQPGAPGADGELGPRGQQGPFGAKGDEGTRGFPGAPGPIGLQGLPGPPGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGIGGEPGKKGPRGERGEKGEAGQPGTAGSTGGRGLPGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGERGEDGEAGESGSPGPPGENGPPGPLGKRGPAGTRGPEGRQGEKGTKGDPGAVGPPGKTGPVGPQGVPGKPGTEGLRGLPGSVGEQGSPGPAGQKGPPGPIGPPGLPGLRGDSGGKGEKGHPGLIGLIGPPGEQGEKGDRGMPGPHGSNGPKGETGMPGGTGPIGPSGPPGLPGPQGIKGAKGSSGGAGPKGEKGVQGPPGLPGPPGEVIQPLPIQRSPKSKRSIDASQLLPENDMPASDATGTEFLMGSEGMEEIFGSLNSLRQEIETMRFPLGTQDSPGRTCQDLHLSQPELKDGEYWIDPNQGCSRDAFKVFCNFTKGAETCLYPSQSISTVKMNSWNKETPGSWYSQFSTGSKFSYVDSNGEPVGVVQLGFLRLLSVQAHQTLTYHCHRSVAWADRNAKNTYSRALHFRGANDEDLSYETNPYVKALVDGCSYRKGFDRTVLEINTPQVEHLPLLDIKVSDFGESNQQFGFEVGPVCFQG

Target ORF information:

RefSeq Version	XM_011617369.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617369.1

ORF Insert Sequence:

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGGC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATTAC 
TTTGATGAGG AGCAGGATGA CCATGAATAC CCTTATTATT ATGAGGAAAC AACAGGCCGT 
CCCTCCTCCT CTGCACCCCC CTCTCCCCAA CCTGGGGCCC CCAGTCAACA GGATGTAGAC 
ACTGAATATT CCGAGGCTGG CCACACCCCT ACAGAGAGAG ATTATTACTA TGAGGAGACG 
GTACCACTGC CAGAGGGTGA GGTGGTTGGG CAAGAGGAAC TCGCCGTACA GCCCCAGGTG 
GAGCCTGTGT TGGAGGGAGA AGAGGTGCAT TCGACCAAGG CTGTCGGTGA GGGAGAGGAG 
GCCTTCACAG AGGAGTATGT GACTGGAGAT GTGACCCTTA AGGAATACGA CTATTCCTAC 
AGGGACTACA ATGAGCCATT AGTGGAGACT GGAGAAGTCG ATGCCAACAT GGGCCCCGCC 
CTCTCTGCTG TGACAGACGA GGGAGGCGCT GGGGTGAGAG GCCAGAAAGG AGAGAAAGGA 
GAACCTGCTG TTTTGGAGCC TGGGATGCTG ATTGAAGGAC CACCAGGACC TGAGGGACCT 
GCTGGCCTAC CTGGTCCTTC TGGCCCTTCT GGACCTCCTG GCTCTGTGGG TGACCCAGGA 
GAGAGGGGCC CTCCTGGTAA GGCTGGTCTT CCTGGAGCTG ATGGAGTCCC TGGACCTCCT 
GGGACCTCTG TTATGCTGCC TTTCCGTTTT GGTCAGAGTG GAGGAGATAA AGGTCCTGCT 
GTTTCTGCTC AGGAAGCTCA GGCAGCAGCC ATCTTGTCTC AGGCCAGGAT GGCTCTTAAA 
GGACCTCCTG GTCCAATGGG ATTCACTGGG CGTCCCGGAC CCTTGGGAAA TCCTGGAAGT 
CCTGGTCTGA AAGGAGAGAG TGGAGACCCT GGACCCCAGG GCCCGAGGGG ATCTCTAGGC 
TTGATGGGAC CTCCAGGCAA ATCAGGAAGA AGAGGTCGCG CTGGAGCCGA CGGTGCCAGA 
GGAATGCCTG GAGAGTCTGG TTCTAAGGGT GATCGTGGTT TTGATGGTCT TCCTGGTTTA 
CCTGGAGACA AAGGACACAG GGGTGACTCG GGACCAATGG GACCTCCAGG ATCTCAGGGA 
GAGGATGGAG AAAGGGGAGA CGACGGAGAC GTTGGACCCC GGGGTCTTCC AGGTGAACCA 
GGACCTCGTG GTTTGCTTGG ACCTAAAGGT CCTCCTGGAA TCCCAGGCCC TCCTGGTGTA 
CGAGGAAGTG ACGGACCCCA CGGTCCAAAA GGAAACTTGG GTCCTCAAGG TGAGCCAGGA 
CCCCCTGGTC AGCAGGGAAC CCCAGGAACT CAGGGAATGC CAGGACCTCA AGGACATACT 
GGACCTCCAG GAGAAAAGGG TCCAACAGGA AAACCGGGTT TACCAGGAAT GCCTGGAGCT 
GATGGCCCTC CTGGTCACCC AGGAAAGGAA GGCCCATCAG GCACCAAAGG AAACCAGGGT 
CCCAGTGGTC CCCAGGGGCC TATTGGCTAT CCTGGTCCTC GTGGCCTCAA GGGCGGTCAA 
GGAATCCGGG GATTGAAGGG TCACAAAGGA GAGAAGGGAG AAGATGGTTT CCCTGGAATT 
AAGGGAGACT TTGGGATCAA GGGTGAGAGG GGTGAGATTG GAGTACCAGG TCCTAGAGGA 
GAGGACGGTC CAGAGGGGCC AAAAGGTCGT TTTGGTCCCC CTGGTGAAAC TGGACCACTC 
GGAGCAGTAG GAGAGAAGGG TAAACTTGGT GTACCGGGAC TTCCTGGATA TCCTGGAAGA 
CAAGGACCAA AGGGATCTCT TGGATTCCCA GGATTCCCTG GATCCAATGG CGAGAAGGGA 
ACAAGGGGTC TCACTGGCAA ATCAGGACCA AGAGGACAAA GAGGACCAAC GGGCCCCAGA 
GGGCAGAGGG GACCGAGGGG CGCAACTGGC AAACTGGGGC CAAAGGGGAC TTCGGGAAGT 
GATGGCCCCC CTGGTCCTCC TGGAGAAAGG GGCTTGCCTG GGCCTCAGGG AGCCAATGGG 
TTCCCCGGAC CTAAAGGGCC ACCGGGGCCA CCAGGGAAGG ACGGGCTGCC TGGACACCCT 
GGGCAGAGAG GAGAAGTGGG TTTCCAAGGT AAAATGGGTC CACCTGGGCC TCCTGGAGTT 
GTTGGACCTC AGGGTCCAGC TGGAGAGACT GGCCCTAATG GTGAACGTGG CCACCCTGGA 
CCACCAGGTC CACCAGGAGA ACAGGGACTT CCTGGTCCCT CAGGAAAGGA GGGAACCAAA 
GGAGACCCTG GACCCCCAGG AGGCCCTGGC AAAGATGGGC CTCCTGGACT GAGGGGCTTC 
CCTGGTGAGA GAGGACTGCC AGGAACACCT GGAGGTGGAG GACTCAAAGG AAGCGAAGGA 
CCTGCTGGAC CTCCTGGACC GGCTGGCTCT CCTGGTGAGA GGGGGCAATC TGGTACCGCT 
GGGCCTGTTG GACCTCCTGG AAGACCAGGT CCTCAGGGAC CTCCTGGACC TGCAGGAGAG 
AAAGGTGTTT CTGGTGAGAA AGGCCCAATT GGCCCAGCTG GTCGTGACGG ACTACAGGGT 
CCTGTGGGTC TGCCTGGCCC AGCTGGACCG CCCGGAGTAC CTGGAGAAGA TGGAGATAAG 
GGGGAGGTTG GAGAACACGG TCAGAAAGGT GCCAAGGGAG GGAAAGGAGA GCATGGTCCT 
CCAGGTCCAC CTGGACCAAT GGGACCAGTC GGCCAGCCTG GTGCTCCAGG TGCTGATGGA 
GAGCTTGGGC CGAGGGGTCA GCAGGGACCT TTTGGAGCTA AAGGGGATGA AGGAACCAGA 
GGATTCCCAG GAGCCCCAGG ACCCATTGGA CTCCAGGGTT TGCCTGGGCC ACCAGGTGAG 
AAGGGAGAAA CCGGAGATGT TGGACCTCTG GGACCTCCAG GCCCTCCAGG ACCCCGTGGC 
CCTGCTGGAC CCAACGGTGC TGATGGCCCT CAAGGGCCTC CTGGTGGTTT GGGTAATCCT 
GGACCTATCG GTGAGAAGGG AGAGCCTGGA GAGTCTGGAC CCCCTGGAAT TGGAGGGGAG 
CCTGGAAAGA AGGGCCCCAG AGGGGAACGT GGGGAGAAGG GGGAGGCTGG ACAACCAGGA 
ACCGCTGGAT CCACTGGAGG ACGTGGACTA CCTGGAGATG ATGGACCCAA AGGAAACCCA 
GGTCCTGTTG GTTTCCCCGG TGATCCTGGT CCACCGGGTG AGGTTGGACC CAGAGGTCAA 
GACGGTGCCA AGGGAGAAAG AGGAGAAGAT GGTGAAGCAG GAGAGTCTGG ATCCCCTGGA 
CCTCCCGGTG AGAACGGACC CCCCGGCCCC CTAGGAAAGA GGGGTCCTGC TGGAACAAGA 
GGACCAGAGG GACGCCAGGG AGAGAAGGGA ACAAAGGGGG ATCCAGGTGC TGTTGGCCCC 
CCAGGCAAAA CAGGCCCTGT GGGTCCACAG GGAGTGCCAG GAAAACCTGG AACAGAAGGC 
CTTAGAGGGC TTCCAGGATC AGTGGGTGAG CAAGGATCTC CAGGACCTGC AGGACAGAAA 
GGACCACCTG GTCCTATTGG ACCTCCCGGT TTACCTGGTC TGCGTGGAGA TTCTGGCGGC 
AAAGGAGAAA AGGGACACCC GGGTCTTATC GGTCTCATTG GACCCCCAGG AGAACAGGGT 
GAGAAAGGAG ACAGAGGCAT GCCTGGGCCC CATGGATCCA ATGGACCAAA AGGAGAGACT 
GGAATGCCTG GAGGCACAGG ACCTATCGGC CCCTCTGGTC CTCCTGGTCT GCCTGGTCCT 
CAAGGTATTA AAGGAGCCAA AGGTTCTTCT GGTGGAGCTG GTCCAAAAGG AGAAAAAGGA 
GTACAAGGAC CCCCTGGACT TCCTGGCCCA CCAGGGGAGG TTATTCAGCC ACTACCCATC 
CAAAGGAGTC CCAAGTCCAA GCGGTCCATT GATGCCAGCC AGCTACTTCC TGAGAATGAC 
ATGCCTGCAT CCGATGCCAC CGGGACAGAA TTCCTGATGG GTAGTGAAGG CATGGAGGAG 
ATCTTTGGCT CCCTCAACTC GCTTCGGCAG GAGATTGAGA CCATGCGCTT CCCTCTGGGC 
ACCCAGGACA GCCCCGGCCG CACCTGCCAA GACCTGCACC TCAGTCAGCC AGAACTCAAA 
GATGGAGAAT ACTGGATTGA CCCCAACCAG GGCTGCTCCA GAGACGCCTT CAAGGTCTTC 
TGTAACTTTA CTAAGGGGGC AGAGACGTGC CTCTACCCCA GCCAGAGCAT CAGCACGGTG 
AAGATGAATT CCTGGAACAA AGAGACTCCA GGTTCCTGGT ACAGTCAGTT CTCCACTGGC 
AGTAAGTTTT CATACGTGGA CTCCAACGGC GAACCTGTGG GTGTGGTTCA GTTGGGTTTC 
CTTCGTCTCT TGAGCGTGCA GGCCCACCAG ACTCTCACAT ACCACTGCCA CCGCTCGGTA 
GCGTGGGCTG ACCGCAACGC CAAGAACACA TACAGCCGAG CTCTGCACTT CAGGGGTGCC 
AACGACGAGG ATCTAAGCTA TGAGACCAAT CCATACGTCA AGGCTTTGGT TGATGGCTGC 
TCTTATCGTA AGGGCTTTGA CCGGACAGTC CTGGAGATCA ACACACCACA GGTGGAACAT 
CTTCCTCTGC TGGATATCAA GGTGTCAGAC TTTGGGGAGA GCAACCAGCA GTTTGGCTTT 
GAAGTGGGAC CTGTCTGTTT CCAAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101066527 ( XM_011617370.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011615672.1 Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X5, mRNA.

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CloneID	OTo22620
Clone ID Related Accession (Same CDS sequence)	XM_011617370.1
Accession Version	XM_011617370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	collagen alpha-2(XI) chain isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071261.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGGC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATGAT 
GTAGACACTG AATATTCCGA GGCTGGCCAC ACCCCTACAG AGAGAGATTA TTACTATGAG 
GAGACGGTAC CACTGCCAGA GGGTGAGGTG GTTGGGCAAG AGGAACTCGC CGTACAGCCC 
CAGGTGGAGC CTGTGTTGGA GGGAGAAGAG GTGCATTCGA CCAAGGCTGT CGGTGAGGGA 
GAGGAGGCCT TCACAGAGGA GTATGTGACT GGAGATGTGA CCCTTAAGGA ATACGACTAT 
TCCTACAGGG ACTACAATGA GCCATTAGTG GAGACTGGAG AAGTCGATGC CAACATGGGC 
CCCGCCCTCT CTGCTGTGAC AGACGAGGGA GGCGCTGGGG TGAGAGGCCA GAAAGGAGAG 
AAAGGAGAAC CTGCTGTTTT GGAGCCTGGG ATGCTGATTG AAGGACCACC AGGACCTGAG 
GGACCTGCTG GCCTACCTGG TCCTTCTGGC CCTTCTGGAC CTCCTGGCTC TGTGGGTGAC 
CCAGGAGAGA GGGGCCCTCC TGGTAAGGCT GGTCTTCCTG GAGCTGATGG AGTCCCTGGA 
CCTCCTGGGA CCTCTGTTAT GCTGCCTTTC CGTTTTGGTC AGAGTGGAGG AGATAAAGGT 
CCTGCTGTTT CTGCTCAGGA AGCTCAGGCA GCAGCCATCT TGTCTCAGGC CAGGATGGCT 
CTTAAAGGAC CTCCTGGTCC AATGGGATTC ACTGGGCGTC CCGGACCCTT GGGAAATCCT 
GGAAGTCCTG GTCTGAAAGG AGAGAGTGGA GACCCTGGAC CCCAGGGCCC GAGGGGATCT 
CTAGGCTTGA TGGGACCTCC AGGCAAATCA GGAAGAAGAG GTCGCGCTGG AGCCGACGGT 
GCCAGAGGAA TGCCTGGAGA GTCTGGTTCT AAGGGTGATC GTGGTTTTGA TGGTCTTCCT 
GGTTTACCTG GAGACAAAGG ACACAGGGGT GACTCGGGAC CAATGGGACC TCCAGGATCT 
CAGGGAGAGG ATGGAGAAAG GGGAGACGAC GGAGACGTTG GACCCCGGGG TCTTCCAGGT 
GAACCAGGAC CTCGTGGTTT GCTTGGACCT AAAGGTCCTC CTGGAATCCC AGGCCCTCCT 
GGTGTACGAG GAAGTGACGG ACCCCACGGT CCAAAAGGAA ACTTGGGTCC TCAAGGTGAG 
CCAGGACCCC CTGGTCAGCA GGGAACCCCA GGAACTCAGG GAATGCCAGG ACCTCAAGGA 
CATACTGGAC CTCCAGGAGA AAAGGGTCCA ACAGGAAAAC CGGGTTTACC AGGAATGCCT 
GGAGCTGATG GCCCTCCTGG TCACCCAGGA AAGGAAGGCC CATCAGGCAC CAAAGGAAAC 
CAGGGTCCCA GTGGTCCCCA GGGGCCTATT GGCTATCCTG GTCCTCGTGG CCTCAAGGGC 
GGTCAAGGAA TCCGGGGATT GAAGGGTCAC AAAGGAGAGA AGGGAGAAGA TGGTTTCCCT 
GGAATTAAGG GAGACTTTGG GATCAAGGGT GAGAGGGGTG AGATTGGAGT ACCAGGTCCT 
AGAGGAGAGG ACGGTCCAGA GGGGCCAAAA GGTCGTTTTG GTCCCCCTGG TGAAACTGGA 
CCACTCGGAG CAGTAGGAGA GAAGGGTAAA CTTGGTGTAC CGGGACTTCC TGGATATCCT 
GGAAGACAAG GACCAAAGGG ATCTCTTGGA TTCCCAGGAT TCCCTGGATC CAATGGCGAG 
AAGGGAACAA GGGGTCTCAC TGGCAAATCA GGACCAAGAG GACAAAGAGG ACCAACGGGC 
CCCAGAGGGC AGAGGGGACC GAGGGGCGCA ACTGGCAAAC TGGGGCCAAA GGGGACTTCG 
GGAAGTGATG GCCCCCCTGG TCCTCCTGGA GAAAGGGGCT TGCCTGGGCC TCAGGGAGCC 
AATGGGTTCC CCGGACCTAA AGGGCCACCG GGGCCACCAG GGAAGGACGG GCTGCCTGGA 
CACCCTGGGC AGAGAGGAGA AGTGGGTTTC CAAGGTAAAA TGGGTCCACC TGGGCCTCCT 
GGAGTTGTTG GACCTCAGGG TCCAGCTGGA GAGACTGGCC CTAATGGTGA ACGTGGCCAC 
CCTGGACCAC CAGGTCCACC AGGAGAACAG GGACTTCCTG GTCCCTCAGG AAAGGAGGGA 
ACCAAAGGAG ACCCTGGACC CCCAGGAGGC CCTGGCAAAG ATGGGCCTCC TGGACTGAGG 
GGCTTCCCTG GTGAGAGAGG ACTGCCAGGA ACACCTGGAG GTGGAGGACT CAAAGGAAGC 
GAAGGACCTG CTGGACCTCC TGGACCGGCT GGCTCTCCTG GTGAGAGGGG GCAATCTGGT 
ACCGCTGGGC CTGTTGGACC TCCTGGAAGA CCAGGTCCTC AGGGACCTCC TGGACCTGCA 
GGAGAGAAAG GTGTTTCTGG TGAGAAAGGC CCAATTGGCC CAGCTGGTCG TGACGGACTA 
CAGGGTCCTG TGGGTCTGCC TGGCCCAGCT GGACCGCCCG GAGTACCTGG AGAAGATGGA 
GATAAGGGGG AGGTTGGAGA ACACGGTCAG AAAGGTGCCA AGGGAGGGAA AGGAGAGCAT 
GGTCCTCCAG GTCCACCTGG ACCAATGGGA CCAGTCGGCC AGCCTGGTGC TCCAGGTGCT 
GATGGAGAGC TTGGGCCGAG GGGTCAGCAG GGACCTTTTG GAGCTAAAGG GGATGAAGGA 
ACCAGAGGAT TCCCAGGAGC CCCAGGACCC ATTGGACTCC AGGGTTTGCC TGGGCCACCA 
GGTGAGAAGG GAGAAACCGG AGATGTTGGA CCTCTGGGAC CTCCAGGCCC TCCAGGACCC 
CGTGGCCCTG CTGGACCCAA CGGTGCTGAT GGCCCTCAAG GGCCTCCTGG TGGTTTGGGT 
AATCCTGGAC CTATCGGTGA GAAGGGAGAG CCTGGAGAGT CTGGACCCCC TGGAATTGGA 
GGGGAGCCTG GAAAGAAGGG CCCCAGAGGG GAACGTGGGG AGAAGGGGGA GGCTGGACAA 
CCAGGAACCG CTGGATCCAC TGGAGGACGT GGACTACCTG GAGATGATGG ACCCAAAGGA 
AACCCAGGTC CTGTTGGTTT CCCCGGTGAT CCTGGTCCAC CGGGTGAGGT TGGACCCAGA 
GGTCAAGACG GTGCCAAGGG AGAAAGAGGA GAAGATGGTG AAGCAGGAGA GTCTGGATCC 
CCTGGACCTC CCGGTGAGAA CGGACCCCCC GGCCCCCTAG GAAAGAGGGG TCCTGCTGGA 
ACAAGAGGAC CAGAGGGACG CCAGGGAGAG AAGGGAACAA AGGGGGATCC AGGTGCTGTT 
GGCCCCCCAG GCAAAACAGG CCCTGTGGGT CCACAGGGAG TGCCAGGAAA ACCTGGAACA 
GAAGGCCTTA GAGGGCTTCC AGGATCAGTG GGTGAGCAAG GATCTCCAGG ACCTGCAGGA 
CAGAAAGGAC CACCTGGTCC TATTGGACCT CCCGGTTTAC CTGGTCTGCG TGGAGATTCT 
GGCGGCAAAG GAGAAAAGGG ACACCCGGGT CTTATCGGTC TCATTGGACC CCCAGGAGAA 
CAGGGTGAGA AAGGAGACAG AGGCATGCCT GGGCCCCATG GATCCAATGG ACCAAAAGGA 
GAGACTGGAA TGCCTGGAGG CACAGGACCT ATCGGCCCCT CTGGTCCTCC TGGTCTGCCT 
GGTCCTCAAG GTATTAAAGG AGCCAAAGGT TCTTCTGGTG GAGCTGGTCC AAAAGGAGAA 
AAAGGAGTAC AAGGACCCCC TGGACTTCCT GGCCCACCAG GGGAGGTTAT TCAGCCACTA 
CCCATCCAAA GGAGTCCCAA GTCCAAGCGG TCCATTGATG CCAGCCAGCT ACTTCCTGAG 
AATGACATGC CTGCATCCGA TGCCACCGGG ACAGAATTCC TGATGGGTAG TGAAGGCATG 
GAGGAGATCT TTGGCTCCCT CAACTCGCTT CGGCAGGAGA TTGAGACCAT GCGCTTCCCT 
CTGGGCACCC AGGACAGCCC CGGCCGCACC TGCCAAGACC TGCACCTCAG TCAGCCAGAA 
CTCAAAGATG GAGAATACTG GATTGACCCC AACCAGGGCT GCTCCAGAGA CGCCTTCAAG 
GTCTTCTGTA ACTTTACTAA GGGGGCAGAG ACGTGCCTCT ACCCCAGCCA GAGCATCAGC 
ACGGTGAAGA TGAATTCCTG GAACAAAGAG ACTCCAGGTT CCTGGTACAG TCAGTTCTCC 
ACTGGCAGTA AGTTTTCATA CGTGGACTCC AACGGCGAAC CTGTGGGTGT GGTTCAGTTG 
GGTTTCCTTC GTCTCTTGAG CGTGCAGGCC CACCAGACTC TCACATACCA CTGCCACCGC 
TCGGTAGCGT GGGCTGACCG CAACGCCAAG AACACATACA GCCGAGCTCT GCACTTCAGG 
GGTGCCAACG ACGAGGATCT AAGCTATGAG ACCAATCCAT ACGTCAAGGC TTTGGTTGAT 
GGCTGCTCTT ATCGTAAGGG CTTTGACCGG ACAGTCCTGG AGATCAACAC ACCACAGGTG 
GAACATCTTC CTCTGCTGGA TATCAAGGTG TCAGACTTTG GGGAGAGCAA CCAGCAGTTT 
GGCTTTGAAG TGGGACCTGT CTGTTTCCAA GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615672.1
CDS	392..5707
Translation	MDTGDCPCRKKRRPWRMDFSSFALVTVMLIVCQTALVRADPVDVLRALQVPSLPEGVKKVPGFCTSRRSSSPDHAYRITKKAQISAPTKQLFSGRFPENFSIMALVKAQAGLQAFLLSIYSEQGIQQLGIEMGRSPVFLYEDQNGKPAPEDYPLFRGVNLADGKWHRIALSVSKKNVTLLLDCKKKMTRPLPRSNSAEVDTNGITVFGARLLDEEVFQGDIQQLLIASNPQAAYDFCEHYSPDCDSPLPKTQAQDPNTYDVDTEYSEAGHTPTERDYYYEETVPLPEGEVVGQEELAVQPQVEPVLEGEEVHSTKAVGEGEEAFTEEYVTGDVTLKEYDYSYRDYNEPLVETGEVDANMGPALSAVTDEGGAGVRGQKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPSGPPGSVGDPGERGPPGKAGLPGADGVPGPPGTSVMLPFRFGQSGGDKGPAVSAQEAQAAAILSQARMALKGPPGPMGFTGRPGPLGNPGSPGLKGESGDPGPQGPRGSLGLMGPPGKSGRRGRAGADGARGMPGESGSKGDRGFDGLPGLPGDKGHRGDSGPMGPPGSQGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGIPGPPGVRGSDGPHGPKGNLGPQGEPGPPGQQGTPGTQGMPGPQGHTGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGPIGYPGPRGLKGGQGIRGLKGHKGEKGEDGFPGIKGDFGIKGERGEIGVPGPRGEDGPEGPKGRFGPPGETGPLGAVGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGSNGEKGTRGLTGKSGPRGQRGPTGPRGQRGPRGATGKLGPKGTSGSDGPPGPPGERGLPGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKMGPPGPPGVVGPQGPAGETGPNGERGHPGPPGPPGEQGLPGPSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGGGGLKGSEGPAGPPGPAGSPGERGQSGTAGPVGPPGRPGPQGPPGPAGEKGVSGEKGPIGPAGRDGLQGPVGLPGPAGPPGVPGEDGDKGEVGEHGQKGAKGGKGEHGPPGPPGPMGPVGQPGAPGADGELGPRGQQGPFGAKGDEGTRGFPGAPGPIGLQGLPGPPGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGIGGEPGKKGPRGERGEKGEAGQPGTAGSTGGRGLPGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGERGEDGEAGESGSPGPPGENGPPGPLGKRGPAGTRGPEGRQGEKGTKGDPGAVGPPGKTGPVGPQGVPGKPGTEGLRGLPGSVGEQGSPGPAGQKGPPGPIGPPGLPGLRGDSGGKGEKGHPGLIGLIGPPGEQGEKGDRGMPGPHGSNGPKGETGMPGGTGPIGPSGPPGLPGPQGIKGAKGSSGGAGPKGEKGVQGPPGLPGPPGEVIQPLPIQRSPKSKRSIDASQLLPENDMPASDATGTEFLMGSEGMEEIFGSLNSLRQEIETMRFPLGTQDSPGRTCQDLHLSQPELKDGEYWIDPNQGCSRDAFKVFCNFTKGAETCLYPSQSISTVKMNSWNKETPGSWYSQFSTGSKFSYVDSNGEPVGVVQLGFLRLLSVQAHQTLTYHCHRSVAWADRNAKNTYSRALHFRGANDEDLSYETNPYVKALVDGCSYRKGFDRTVLEINTPQVEHLPLLDIKVSDFGESNQQFGFEVGPVCFQG

Target ORF information:

RefSeq Version	XM_011617370.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617370.1

ORF Insert Sequence:

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGGC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATGAT 
GTAGACACTG AATATTCCGA GGCTGGCCAC ACCCCTACAG AGAGAGATTA TTACTATGAG 
GAGACGGTAC CACTGCCAGA GGGTGAGGTG GTTGGGCAAG AGGAACTCGC CGTACAGCCC 
CAGGTGGAGC CTGTGTTGGA GGGAGAAGAG GTGCATTCGA CCAAGGCTGT CGGTGAGGGA 
GAGGAGGCCT TCACAGAGGA GTATGTGACT GGAGATGTGA CCCTTAAGGA ATACGACTAT 
TCCTACAGGG ACTACAATGA GCCATTAGTG GAGACTGGAG AAGTCGATGC CAACATGGGC 
CCCGCCCTCT CTGCTGTGAC AGACGAGGGA GGCGCTGGGG TGAGAGGCCA GAAAGGAGAG 
AAAGGAGAAC CTGCTGTTTT GGAGCCTGGG ATGCTGATTG AAGGACCACC AGGACCTGAG 
GGACCTGCTG GCCTACCTGG TCCTTCTGGC CCTTCTGGAC CTCCTGGCTC TGTGGGTGAC 
CCAGGAGAGA GGGGCCCTCC TGGTAAGGCT GGTCTTCCTG GAGCTGATGG AGTCCCTGGA 
CCTCCTGGGA CCTCTGTTAT GCTGCCTTTC CGTTTTGGTC AGAGTGGAGG AGATAAAGGT 
CCTGCTGTTT CTGCTCAGGA AGCTCAGGCA GCAGCCATCT TGTCTCAGGC CAGGATGGCT 
CTTAAAGGAC CTCCTGGTCC AATGGGATTC ACTGGGCGTC CCGGACCCTT GGGAAATCCT 
GGAAGTCCTG GTCTGAAAGG AGAGAGTGGA GACCCTGGAC CCCAGGGCCC GAGGGGATCT 
CTAGGCTTGA TGGGACCTCC AGGCAAATCA GGAAGAAGAG GTCGCGCTGG AGCCGACGGT 
GCCAGAGGAA TGCCTGGAGA GTCTGGTTCT AAGGGTGATC GTGGTTTTGA TGGTCTTCCT 
GGTTTACCTG GAGACAAAGG ACACAGGGGT GACTCGGGAC CAATGGGACC TCCAGGATCT 
CAGGGAGAGG ATGGAGAAAG GGGAGACGAC GGAGACGTTG GACCCCGGGG TCTTCCAGGT 
GAACCAGGAC CTCGTGGTTT GCTTGGACCT AAAGGTCCTC CTGGAATCCC AGGCCCTCCT 
GGTGTACGAG GAAGTGACGG ACCCCACGGT CCAAAAGGAA ACTTGGGTCC TCAAGGTGAG 
CCAGGACCCC CTGGTCAGCA GGGAACCCCA GGAACTCAGG GAATGCCAGG ACCTCAAGGA 
CATACTGGAC CTCCAGGAGA AAAGGGTCCA ACAGGAAAAC CGGGTTTACC AGGAATGCCT 
GGAGCTGATG GCCCTCCTGG TCACCCAGGA AAGGAAGGCC CATCAGGCAC CAAAGGAAAC 
CAGGGTCCCA GTGGTCCCCA GGGGCCTATT GGCTATCCTG GTCCTCGTGG CCTCAAGGGC 
GGTCAAGGAA TCCGGGGATT GAAGGGTCAC AAAGGAGAGA AGGGAGAAGA TGGTTTCCCT 
GGAATTAAGG GAGACTTTGG GATCAAGGGT GAGAGGGGTG AGATTGGAGT ACCAGGTCCT 
AGAGGAGAGG ACGGTCCAGA GGGGCCAAAA GGTCGTTTTG GTCCCCCTGG TGAAACTGGA 
CCACTCGGAG CAGTAGGAGA GAAGGGTAAA CTTGGTGTAC CGGGACTTCC TGGATATCCT 
GGAAGACAAG GACCAAAGGG ATCTCTTGGA TTCCCAGGAT TCCCTGGATC CAATGGCGAG 
AAGGGAACAA GGGGTCTCAC TGGCAAATCA GGACCAAGAG GACAAAGAGG ACCAACGGGC 
CCCAGAGGGC AGAGGGGACC GAGGGGCGCA ACTGGCAAAC TGGGGCCAAA GGGGACTTCG 
GGAAGTGATG GCCCCCCTGG TCCTCCTGGA GAAAGGGGCT TGCCTGGGCC TCAGGGAGCC 
AATGGGTTCC CCGGACCTAA AGGGCCACCG GGGCCACCAG GGAAGGACGG GCTGCCTGGA 
CACCCTGGGC AGAGAGGAGA AGTGGGTTTC CAAGGTAAAA TGGGTCCACC TGGGCCTCCT 
GGAGTTGTTG GACCTCAGGG TCCAGCTGGA GAGACTGGCC CTAATGGTGA ACGTGGCCAC 
CCTGGACCAC CAGGTCCACC AGGAGAACAG GGACTTCCTG GTCCCTCAGG AAAGGAGGGA 
ACCAAAGGAG ACCCTGGACC CCCAGGAGGC CCTGGCAAAG ATGGGCCTCC TGGACTGAGG 
GGCTTCCCTG GTGAGAGAGG ACTGCCAGGA ACACCTGGAG GTGGAGGACT CAAAGGAAGC 
GAAGGACCTG CTGGACCTCC TGGACCGGCT GGCTCTCCTG GTGAGAGGGG GCAATCTGGT 
ACCGCTGGGC CTGTTGGACC TCCTGGAAGA CCAGGTCCTC AGGGACCTCC TGGACCTGCA 
GGAGAGAAAG GTGTTTCTGG TGAGAAAGGC CCAATTGGCC CAGCTGGTCG TGACGGACTA 
CAGGGTCCTG TGGGTCTGCC TGGCCCAGCT GGACCGCCCG GAGTACCTGG AGAAGATGGA 
GATAAGGGGG AGGTTGGAGA ACACGGTCAG AAAGGTGCCA AGGGAGGGAA AGGAGAGCAT 
GGTCCTCCAG GTCCACCTGG ACCAATGGGA CCAGTCGGCC AGCCTGGTGC TCCAGGTGCT 
GATGGAGAGC TTGGGCCGAG GGGTCAGCAG GGACCTTTTG GAGCTAAAGG GGATGAAGGA 
ACCAGAGGAT TCCCAGGAGC CCCAGGACCC ATTGGACTCC AGGGTTTGCC TGGGCCACCA 
GGTGAGAAGG GAGAAACCGG AGATGTTGGA CCTCTGGGAC CTCCAGGCCC TCCAGGACCC 
CGTGGCCCTG CTGGACCCAA CGGTGCTGAT GGCCCTCAAG GGCCTCCTGG TGGTTTGGGT 
AATCCTGGAC CTATCGGTGA GAAGGGAGAG CCTGGAGAGT CTGGACCCCC TGGAATTGGA 
GGGGAGCCTG GAAAGAAGGG CCCCAGAGGG GAACGTGGGG AGAAGGGGGA GGCTGGACAA 
CCAGGAACCG CTGGATCCAC TGGAGGACGT GGACTACCTG GAGATGATGG ACCCAAAGGA 
AACCCAGGTC CTGTTGGTTT CCCCGGTGAT CCTGGTCCAC CGGGTGAGGT TGGACCCAGA 
GGTCAAGACG GTGCCAAGGG AGAAAGAGGA GAAGATGGTG AAGCAGGAGA GTCTGGATCC 
CCTGGACCTC CCGGTGAGAA CGGACCCCCC GGCCCCCTAG GAAAGAGGGG TCCTGCTGGA 
ACAAGAGGAC CAGAGGGACG CCAGGGAGAG AAGGGAACAA AGGGGGATCC AGGTGCTGTT 
GGCCCCCCAG GCAAAACAGG CCCTGTGGGT CCACAGGGAG TGCCAGGAAA ACCTGGAACA 
GAAGGCCTTA GAGGGCTTCC AGGATCAGTG GGTGAGCAAG GATCTCCAGG ACCTGCAGGA 
CAGAAAGGAC CACCTGGTCC TATTGGACCT CCCGGTTTAC CTGGTCTGCG TGGAGATTCT 
GGCGGCAAAG GAGAAAAGGG ACACCCGGGT CTTATCGGTC TCATTGGACC CCCAGGAGAA 
CAGGGTGAGA AAGGAGACAG AGGCATGCCT GGGCCCCATG GATCCAATGG ACCAAAAGGA 
GAGACTGGAA TGCCTGGAGG CACAGGACCT ATCGGCCCCT CTGGTCCTCC TGGTCTGCCT 
GGTCCTCAAG GTATTAAAGG AGCCAAAGGT TCTTCTGGTG GAGCTGGTCC AAAAGGAGAA 
AAAGGAGTAC AAGGACCCCC TGGACTTCCT GGCCCACCAG GGGAGGTTAT TCAGCCACTA 
CCCATCCAAA GGAGTCCCAA GTCCAAGCGG TCCATTGATG CCAGCCAGCT ACTTCCTGAG 
AATGACATGC CTGCATCCGA TGCCACCGGG ACAGAATTCC TGATGGGTAG TGAAGGCATG 
GAGGAGATCT TTGGCTCCCT CAACTCGCTT CGGCAGGAGA TTGAGACCAT GCGCTTCCCT 
CTGGGCACCC AGGACAGCCC CGGCCGCACC TGCCAAGACC TGCACCTCAG TCAGCCAGAA 
CTCAAAGATG GAGAATACTG GATTGACCCC AACCAGGGCT GCTCCAGAGA CGCCTTCAAG 
GTCTTCTGTA ACTTTACTAA GGGGGCAGAG ACGTGCCTCT ACCCCAGCCA GAGCATCAGC 
ACGGTGAAGA TGAATTCCTG GAACAAAGAG ACTCCAGGTT CCTGGTACAG TCAGTTCTCC 
ACTGGCAGTA AGTTTTCATA CGTGGACTCC AACGGCGAAC CTGTGGGTGT GGTTCAGTTG 
GGTTTCCTTC GTCTCTTGAG CGTGCAGGCC CACCAGACTC TCACATACCA CTGCCACCGC 
TCGGTAGCGT GGGCTGACCG CAACGCCAAG AACACATACA GCCGAGCTCT GCACTTCAGG 
GGTGCCAACG ACGAGGATCT AAGCTATGAG ACCAATCCAT ACGTCAAGGC TTTGGTTGAT 
GGCTGCTCTT ATCGTAAGGG CTTTGACCGG ACAGTCCTGG AGATCAACAC ACCACAGGTG 
GAACATCTTC CTCTGCTGGA TATCAAGGTG TCAGACTTTG GGGAGAGCAA CCAGCAGTTT 
GGCTTTGAAG TGGGACCTGT CTGTTTCCAA GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101066527 ( XM_011617371.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011615673.1 Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X6, mRNA.

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CloneID	OTo22621
Clone ID Related Accession (Same CDS sequence)	XM_011617371.1
Accession Version	XM_011617371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	collagen alpha-2(XI) chain isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071261.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGGC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATCCC 
CAGGTGGAGC CTGTGTTGGA GGGAGAAGAG GTGCATTCGA CCAAGGCTGT CGGTGAGGGA 
GAGGAGGCCT TCACAGAGGA GTATGTGACT GGAGATGTGA CCCTTAAGGA ATACGACTAT 
TCCTACAGGG ACTACAATGA GCCATTAGTG GAGACTGGAG AAGTCGATGC CAACATGGGC 
CCCGCCCTCT CTGCTGTGAC AGACGAGGGA GGCGCTGGGG TGAGAGGCCA GAAAGGAGAG 
AAAGGAGAAC CTGCTGTTTT GGAGCCTGGG ATGCTGATTG AAGGACCACC AGGACCTGAG 
GGACCTGCTG GCCTACCTGG TCCTTCTGGC CCTTCTGGAC CTCCTGGCTC TGTGGGTGAC 
CCAGGAGAGA GGGGCCCTCC TGGTAAGGCT GGTCTTCCTG GAGCTGATGG AGTCCCTGGA 
CCTCCTGGGA CCTCTGTTAT GCTGCCTTTC CGTTTTGGTC AGAGTGGAGG AGATAAAGGT 
CCTGCTGTTT CTGCTCAGGA AGCTCAGGCA GCAGCCATCT TGTCTCAGGC CAGGATGGCT 
CTTAAAGGAC CTCCTGGTCC AATGGGATTC ACTGGGCGTC CCGGACCCTT GGGAAATCCT 
GGAAGTCCTG GTCTGAAAGG AGAGAGTGGA GACCCTGGAC CCCAGGGCCC GAGGGGATCT 
CTAGGCTTGA TGGGACCTCC AGGCAAATCA GGAAGAAGAG GTCGCGCTGG AGCCGACGGT 
GCCAGAGGAA TGCCTGGAGA GTCTGGTTCT AAGGGTGATC GTGGTTTTGA TGGTCTTCCT 
GGTTTACCTG GAGACAAAGG ACACAGGGGT GACTCGGGAC CAATGGGACC TCCAGGATCT 
CAGGGAGAGG ATGGAGAAAG GGGAGACGAC GGAGACGTTG GACCCCGGGG TCTTCCAGGT 
GAACCAGGAC CTCGTGGTTT GCTTGGACCT AAAGGTCCTC CTGGAATCCC AGGCCCTCCT 
GGTGTACGAG GAAGTGACGG ACCCCACGGT CCAAAAGGAA ACTTGGGTCC TCAAGGTGAG 
CCAGGACCCC CTGGTCAGCA GGGAACCCCA GGAACTCAGG GAATGCCAGG ACCTCAAGGA 
CATACTGGAC CTCCAGGAGA AAAGGGTCCA ACAGGAAAAC CGGGTTTACC AGGAATGCCT 
GGAGCTGATG GCCCTCCTGG TCACCCAGGA AAGGAAGGCC CATCAGGCAC CAAAGGAAAC 
CAGGGTCCCA GTGGTCCCCA GGGGCCTATT GGCTATCCTG GTCCTCGTGG CCTCAAGGGC 
GGTCAAGGAA TCCGGGGATT GAAGGGTCAC AAAGGAGAGA AGGGAGAAGA TGGTTTCCCT 
GGAATTAAGG GAGACTTTGG GATCAAGGGT GAGAGGGGTG AGATTGGAGT ACCAGGTCCT 
AGAGGAGAGG ACGGTCCAGA GGGGCCAAAA GGTCGTTTTG GTCCCCCTGG TGAAACTGGA 
CCACTCGGAG CAGTAGGAGA GAAGGGTAAA CTTGGTGTAC CGGGACTTCC TGGATATCCT 
GGAAGACAAG GACCAAAGGG ATCTCTTGGA TTCCCAGGAT TCCCTGGATC CAATGGCGAG 
AAGGGAACAA GGGGTCTCAC TGGCAAATCA GGACCAAGAG GACAAAGAGG ACCAACGGGC 
CCCAGAGGGC AGAGGGGACC GAGGGGCGCA ACTGGCAAAC TGGGGCCAAA GGGGACTTCG 
GGAAGTGATG GCCCCCCTGG TCCTCCTGGA GAAAGGGGCT TGCCTGGGCC TCAGGGAGCC 
AATGGGTTCC CCGGACCTAA AGGGCCACCG GGGCCACCAG GGAAGGACGG GCTGCCTGGA 
CACCCTGGGC AGAGAGGAGA AGTGGGTTTC CAAGGTAAAA TGGGTCCACC TGGGCCTCCT 
GGAGTTGTTG GACCTCAGGG TCCAGCTGGA GAGACTGGCC CTAATGGTGA ACGTGGCCAC 
CCTGGACCAC CAGGTCCACC AGGAGAACAG GGACTTCCTG GTCCCTCAGG AAAGGAGGGA 
ACCAAAGGAG ACCCTGGACC CCCAGGAGGC CCTGGCAAAG ATGGGCCTCC TGGACTGAGG 
GGCTTCCCTG GTGAGAGAGG ACTGCCAGGA ACACCTGGAG GTGGAGGACT CAAAGGAAGC 
GAAGGACCTG CTGGACCTCC TGGACCGGCT GGCTCTCCTG GTGAGAGGGG GCAATCTGGT 
ACCGCTGGGC CTGTTGGACC TCCTGGAAGA CCAGGTCCTC AGGGACCTCC TGGACCTGCA 
GGAGAGAAAG GTGTTTCTGG TGAGAAAGGC CCAATTGGCC CAGCTGGTCG TGACGGACTA 
CAGGGTCCTG TGGGTCTGCC TGGCCCAGCT GGACCGCCCG GAGTACCTGG AGAAGATGGA 
GATAAGGGGG AGGTTGGAGA ACACGGTCAG AAAGGTGCCA AGGGAGGGAA AGGAGAGCAT 
GGTCCTCCAG GTCCACCTGG ACCAATGGGA CCAGTCGGCC AGCCTGGTGC TCCAGGTGCT 
GATGGAGAGC TTGGGCCGAG GGGTCAGCAG GGACCTTTTG GAGCTAAAGG GGATGAAGGA 
ACCAGAGGAT TCCCAGGAGC CCCAGGACCC ATTGGACTCC AGGGTTTGCC TGGGCCACCA 
GGTGAGAAGG GAGAAACCGG AGATGTTGGA CCTCTGGGAC CTCCAGGCCC TCCAGGACCC 
CGTGGCCCTG CTGGACCCAA CGGTGCTGAT GGCCCTCAAG GGCCTCCTGG TGGTTTGGGT 
AATCCTGGAC CTATCGGTGA GAAGGGAGAG CCTGGAGAGT CTGGACCCCC TGGAATTGGA 
GGGGAGCCTG GAAAGAAGGG CCCCAGAGGG GAACGTGGGG AGAAGGGGGA GGCTGGACAA 
CCAGGAACCG CTGGATCCAC TGGAGGACGT GGACTACCTG GAGATGATGG ACCCAAAGGA 
AACCCAGGTC CTGTTGGTTT CCCCGGTGAT CCTGGTCCAC CGGGTGAGGT TGGACCCAGA 
GGTCAAGACG GTGCCAAGGG AGAAAGAGGA GAAGATGGTG AAGCAGGAGA GTCTGGATCC 
CCTGGACCTC CCGGTGAGAA CGGACCCCCC GGCCCCCTAG GAAAGAGGGG TCCTGCTGGA 
ACAAGAGGAC CAGAGGGACG CCAGGGAGAG AAGGGAACAA AGGGGGATCC AGGTGCTGTT 
GGCCCCCCAG GCAAAACAGG CCCTGTGGGT CCACAGGGAG TGCCAGGAAA ACCTGGAACA 
GAAGGCCTTA GAGGGCTTCC AGGATCAGTG GGTGAGCAAG GATCTCCAGG ACCTGCAGGA 
CAGAAAGGAC CACCTGGTCC TATTGGACCT CCCGGTTTAC CTGGTCTGCG TGGAGATTCT 
GGCGGCAAAG GAGAAAAGGG ACACCCGGGT CTTATCGGTC TCATTGGACC CCCAGGAGAA 
CAGGGTGAGA AAGGAGACAG AGGCATGCCT GGGCCCCATG GATCCAATGG ACCAAAAGGA 
GAGACTGGAA TGCCTGGAGG CACAGGACCT ATCGGCCCCT CTGGTCCTCC TGGTCTGCCT 
GGTCCTCAAG GTATTAAAGG AGCCAAAGGT TCTTCTGGTG GAGCTGGTCC AAAAGGAGAA 
AAAGGAGTAC AAGGACCCCC TGGACTTCCT GGCCCACCAG GGGAGGTTAT TCAGCCACTA 
CCCATCCAAA GGAGTCCCAA GTCCAAGCGG TCCATTGATG CCAGCCAGCT ACTTCCTGAG 
AATGACATGC CTGCATCCGA TGCCACCGGG ACAGAATTCC TGATGGGTAG TGAAGGCATG 
GAGGAGATCT TTGGCTCCCT CAACTCGCTT CGGCAGGAGA TTGAGACCAT GCGCTTCCCT 
CTGGGCACCC AGGACAGCCC CGGCCGCACC TGCCAAGACC TGCACCTCAG TCAGCCAGAA 
CTCAAAGATG GAGAATACTG GATTGACCCC AACCAGGGCT GCTCCAGAGA CGCCTTCAAG 
GTCTTCTGTA ACTTTACTAA GGGGGCAGAG ACGTGCCTCT ACCCCAGCCA GAGCATCAGC 
ACGGTGAAGA TGAATTCCTG GAACAAAGAG ACTCCAGGTT CCTGGTACAG TCAGTTCTCC 
ACTGGCAGTA AGTTTTCATA CGTGGACTCC AACGGCGAAC CTGTGGGTGT GGTTCAGTTG 
GGTTTCCTTC GTCTCTTGAG CGTGCAGGCC CACCAGACTC TCACATACCA CTGCCACCGC 
TCGGTAGCGT GGGCTGACCG CAACGCCAAG AACACATACA GCCGAGCTCT GCACTTCAGG 
GGTGCCAACG ACGAGGATCT AAGCTATGAG ACCAATCCAT ACGTCAAGGC TTTGGTTGAT 
GGCTGCTCTT ATCGTAAGGG CTTTGACCGG ACAGTCCTGG AGATCAACAC ACCACAGGTG 
GAACATCTTC CTCTGCTGGA TATCAAGGTG TCAGACTTTG GGGAGAGCAA CCAGCAGTTT 
GGCTTTGAAG TGGGACCTGT CTGTTTCCAA GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011615673.1
CDS	392..5587
Translation	MDTGDCPCRKKRRPWRMDFSSFALVTVMLIVCQTALVRADPVDVLRALQVPSLPEGVKKVPGFCTSRRSSSPDHAYRITKKAQISAPTKQLFSGRFPENFSIMALVKAQAGLQAFLLSIYSEQGIQQLGIEMGRSPVFLYEDQNGKPAPEDYPLFRGVNLADGKWHRIALSVSKKNVTLLLDCKKKMTRPLPRSNSAEVDTNGITVFGARLLDEEVFQGDIQQLLIASNPQAAYDFCEHYSPDCDSPLPKTQAQDPNTYPQVEPVLEGEEVHSTKAVGEGEEAFTEEYVTGDVTLKEYDYSYRDYNEPLVETGEVDANMGPALSAVTDEGGAGVRGQKGEKGEPAVLEPGMLIEGPPGPEGPAGLPGPSGPSGPPGSVGDPGERGPPGKAGLPGADGVPGPPGTSVMLPFRFGQSGGDKGPAVSAQEAQAAAILSQARMALKGPPGPMGFTGRPGPLGNPGSPGLKGESGDPGPQGPRGSLGLMGPPGKSGRRGRAGADGARGMPGESGSKGDRGFDGLPGLPGDKGHRGDSGPMGPPGSQGEDGERGDDGDVGPRGLPGEPGPRGLLGPKGPPGIPGPPGVRGSDGPHGPKGNLGPQGEPGPPGQQGTPGTQGMPGPQGHTGPPGEKGPTGKPGLPGMPGADGPPGHPGKEGPSGTKGNQGPSGPQGPIGYPGPRGLKGGQGIRGLKGHKGEKGEDGFPGIKGDFGIKGERGEIGVPGPRGEDGPEGPKGRFGPPGETGPLGAVGEKGKLGVPGLPGYPGRQGPKGSLGFPGFPGSNGEKGTRGLTGKSGPRGQRGPTGPRGQRGPRGATGKLGPKGTSGSDGPPGPPGERGLPGPQGANGFPGPKGPPGPPGKDGLPGHPGQRGEVGFQGKMGPPGPPGVVGPQGPAGETGPNGERGHPGPPGPPGEQGLPGPSGKEGTKGDPGPPGGPGKDGPPGLRGFPGERGLPGTPGGGGLKGSEGPAGPPGPAGSPGERGQSGTAGPVGPPGRPGPQGPPGPAGEKGVSGEKGPIGPAGRDGLQGPVGLPGPAGPPGVPGEDGDKGEVGEHGQKGAKGGKGEHGPPGPPGPMGPVGQPGAPGADGELGPRGQQGPFGAKGDEGTRGFPGAPGPIGLQGLPGPPGEKGETGDVGPLGPPGPPGPRGPAGPNGADGPQGPPGGLGNPGPIGEKGEPGESGPPGIGGEPGKKGPRGERGEKGEAGQPGTAGSTGGRGLPGDDGPKGNPGPVGFPGDPGPPGEVGPRGQDGAKGERGEDGEAGESGSPGPPGENGPPGPLGKRGPAGTRGPEGRQGEKGTKGDPGAVGPPGKTGPVGPQGVPGKPGTEGLRGLPGSVGEQGSPGPAGQKGPPGPIGPPGLPGLRGDSGGKGEKGHPGLIGLIGPPGEQGEKGDRGMPGPHGSNGPKGETGMPGGTGPIGPSGPPGLPGPQGIKGAKGSSGGAGPKGEKGVQGPPGLPGPPGEVIQPLPIQRSPKSKRSIDASQLLPENDMPASDATGTEFLMGSEGMEEIFGSLNSLRQEIETMRFPLGTQDSPGRTCQDLHLSQPELKDGEYWIDPNQGCSRDAFKVFCNFTKGAETCLYPSQSISTVKMNSWNKETPGSWYSQFSTGSKFSYVDSNGEPVGVVQLGFLRLLSVQAHQTLTYHCHRSVAWADRNAKNTYSRALHFRGANDEDLSYETNPYVKALVDGCSYRKGFDRTVLEINTPQVEHLPLLDIKVSDFGESNQQFGFEVGPVCFQG

Target ORF information:

RefSeq Version	XM_011617371.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes collagen, type XI, alpha 2 (col11a2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011617371.1

ORF Insert Sequence:

ATGGATACTG GAGACTGTCC CTGTCGGAAG AAGCGGAGAC CATGGCGCAT GGACTTCTCC 
TCCTTCGCTT TGGTAACCGT GATGCTCATC GTGTGCCAAA CAGCCTTGGT TCGGGCAGAC 
CCAGTTGATG TGTTGAGGGC TCTGCAGGTC CCATCTCTGC CCGAGGGTGT GAAGAAGGTT 
CCTGGTTTCT GCACATCCCG ACGCTCCAGC AGCCCCGATC ATGCTTACCG TATTACCAAA 
AAGGCCCAAA TCTCTGCTCC CACCAAGCAG CTCTTCTCAG GTCGATTCCC TGAAAACTTT 
TCCATCATGG CTCTGGTCAA GGCCCAGGCT GGACTCCAGG CCTTCCTCCT GTCCATCTAC 
AGTGAGCAGG GTATCCAACA GCTGGGCATT GAAATGGGGC GTTCACCTGT CTTTCTGTAC 
GAGGACCAGA ACGGCAAGCC AGCCCCCGAG GACTACCCAC TGTTCAGAGG CGTCAACCTG 
GCTGATGGCA AGTGGCACCG CATTGCTCTC TCTGTCTCAA AGAAGAATGT GACACTGCTG 
CTGGACTGCA AGAAGAAAAT GACCCGACCC CTTCCACGCA GCAACAGCGC AGAGGTCGAC 
ACTAACGGCA TCACGGTGTT CGGAGCCCGT CTGCTGGATG AGGAGGTTTT CCAGGGAGAC 
ATCCAGCAGC TGCTGATCGC CTCCAACCCT CAGGCTGCCT ATGACTTCTG TGAGCACTAC 
AGCCCTGACT GTGACTCCCC TCTGCCCAAA ACACAGGCGC AGGACCCCAA CACCTATCCC 
CAGGTGGAGC CTGTGTTGGA GGGAGAAGAG GTGCATTCGA CCAAGGCTGT CGGTGAGGGA 
GAGGAGGCCT TCACAGAGGA GTATGTGACT GGAGATGTGA CCCTTAAGGA ATACGACTAT 
TCCTACAGGG ACTACAATGA GCCATTAGTG GAGACTGGAG AAGTCGATGC CAACATGGGC 
CCCGCCCTCT CTGCTGTGAC AGACGAGGGA GGCGCTGGGG TGAGAGGCCA GAAAGGAGAG 
AAAGGAGAAC CTGCTGTTTT GGAGCCTGGG ATGCTGATTG AAGGACCACC AGGACCTGAG 
GGACCTGCTG GCCTACCTGG TCCTTCTGGC CCTTCTGGAC CTCCTGGCTC TGTGGGTGAC 
CCAGGAGAGA GGGGCCCTCC TGGTAAGGCT GGTCTTCCTG GAGCTGATGG AGTCCCTGGA 
CCTCCTGGGA CCTCTGTTAT GCTGCCTTTC CGTTTTGGTC AGAGTGGAGG AGATAAAGGT 
CCTGCTGTTT CTGCTCAGGA AGCTCAGGCA GCAGCCATCT TGTCTCAGGC CAGGATGGCT 
CTTAAAGGAC CTCCTGGTCC AATGGGATTC ACTGGGCGTC CCGGACCCTT GGGAAATCCT 
GGAAGTCCTG GTCTGAAAGG AGAGAGTGGA GACCCTGGAC CCCAGGGCCC GAGGGGATCT 
CTAGGCTTGA TGGGACCTCC AGGCAAATCA GGAAGAAGAG GTCGCGCTGG AGCCGACGGT 
GCCAGAGGAA TGCCTGGAGA GTCTGGTTCT AAGGGTGATC GTGGTTTTGA TGGTCTTCCT 
GGTTTACCTG GAGACAAAGG ACACAGGGGT GACTCGGGAC CAATGGGACC TCCAGGATCT 
CAGGGAGAGG ATGGAGAAAG GGGAGACGAC GGAGACGTTG GACCCCGGGG TCTTCCAGGT 
GAACCAGGAC CTCGTGGTTT GCTTGGACCT AAAGGTCCTC CTGGAATCCC AGGCCCTCCT 
GGTGTACGAG GAAGTGACGG ACCCCACGGT CCAAAAGGAA ACTTGGGTCC TCAAGGTGAG 
CCAGGACCCC CTGGTCAGCA GGGAACCCCA GGAACTCAGG GAATGCCAGG ACCTCAAGGA 
CATACTGGAC CTCCAGGAGA AAAGGGTCCA ACAGGAAAAC CGGGTTTACC AGGAATGCCT 
GGAGCTGATG GCCCTCCTGG TCACCCAGGA AAGGAAGGCC CATCAGGCAC CAAAGGAAAC 
CAGGGTCCCA GTGGTCCCCA GGGGCCTATT GGCTATCCTG GTCCTCGTGG CCTCAAGGGC 
GGTCAAGGAA TCCGGGGATT GAAGGGTCAC AAAGGAGAGA AGGGAGAAGA TGGTTTCCCT 
GGAATTAAGG GAGACTTTGG GATCAAGGGT GAGAGGGGTG AGATTGGAGT ACCAGGTCCT 
AGAGGAGAGG ACGGTCCAGA GGGGCCAAAA GGTCGTTTTG GTCCCCCTGG TGAAACTGGA 
CCACTCGGAG CAGTAGGAGA GAAGGGTAAA CTTGGTGTAC CGGGACTTCC TGGATATCCT 
GGAAGACAAG GACCAAAGGG ATCTCTTGGA TTCCCAGGAT TCCCTGGATC CAATGGCGAG 
AAGGGAACAA GGGGTCTCAC TGGCAAATCA GGACCAAGAG GACAAAGAGG ACCAACGGGC 
CCCAGAGGGC AGAGGGGACC GAGGGGCGCA ACTGGCAAAC TGGGGCCAAA GGGGACTTCG 
GGAAGTGATG GCCCCCCTGG TCCTCCTGGA GAAAGGGGCT TGCCTGGGCC TCAGGGAGCC 
AATGGGTTCC CCGGACCTAA AGGGCCACCG GGGCCACCAG GGAAGGACGG GCTGCCTGGA 
CACCCTGGGC AGAGAGGAGA AGTGGGTTTC CAAGGTAAAA TGGGTCCACC TGGGCCTCCT 
GGAGTTGTTG GACCTCAGGG TCCAGCTGGA GAGACTGGCC CTAATGGTGA ACGTGGCCAC 
CCTGGACCAC CAGGTCCACC AGGAGAACAG GGACTTCCTG GTCCCTCAGG AAAGGAGGGA 
ACCAAAGGAG ACCCTGGACC CCCAGGAGGC CCTGGCAAAG ATGGGCCTCC TGGACTGAGG 
GGCTTCCCTG GTGAGAGAGG ACTGCCAGGA ACACCTGGAG GTGGAGGACT CAAAGGAAGC 
GAAGGACCTG CTGGACCTCC TGGACCGGCT GGCTCTCCTG GTGAGAGGGG GCAATCTGGT 
ACCGCTGGGC CTGTTGGACC TCCTGGAAGA CCAGGTCCTC AGGGACCTCC TGGACCTGCA 
GGAGAGAAAG GTGTTTCTGG TGAGAAAGGC CCAATTGGCC CAGCTGGTCG TGACGGACTA 
CAGGGTCCTG TGGGTCTGCC TGGCCCAGCT GGACCGCCCG GAGTACCTGG AGAAGATGGA 
GATAAGGGGG AGGTTGGAGA ACACGGTCAG AAAGGTGCCA AGGGAGGGAA AGGAGAGCAT 
GGTCCTCCAG GTCCACCTGG ACCAATGGGA CCAGTCGGCC AGCCTGGTGC TCCAGGTGCT 
GATGGAGAGC TTGGGCCGAG GGGTCAGCAG GGACCTTTTG GAGCTAAAGG GGATGAAGGA 
ACCAGAGGAT TCCCAGGAGC CCCAGGACCC ATTGGACTCC AGGGTTTGCC TGGGCCACCA 
GGTGAGAAGG GAGAAACCGG AGATGTTGGA CCTCTGGGAC CTCCAGGCCC TCCAGGACCC 
CGTGGCCCTG CTGGACCCAA CGGTGCTGAT GGCCCTCAAG GGCCTCCTGG TGGTTTGGGT 
AATCCTGGAC CTATCGGTGA GAAGGGAGAG CCTGGAGAGT CTGGACCCCC TGGAATTGGA 
GGGGAGCCTG GAAAGAAGGG CCCCAGAGGG GAACGTGGGG AGAAGGGGGA GGCTGGACAA 
CCAGGAACCG CTGGATCCAC TGGAGGACGT GGACTACCTG GAGATGATGG ACCCAAAGGA 
AACCCAGGTC CTGTTGGTTT CCCCGGTGAT CCTGGTCCAC CGGGTGAGGT TGGACCCAGA 
GGTCAAGACG GTGCCAAGGG AGAAAGAGGA GAAGATGGTG AAGCAGGAGA GTCTGGATCC 
CCTGGACCTC CCGGTGAGAA CGGACCCCCC GGCCCCCTAG GAAAGAGGGG TCCTGCTGGA 
ACAAGAGGAC CAGAGGGACG CCAGGGAGAG AAGGGAACAA AGGGGGATCC AGGTGCTGTT 
GGCCCCCCAG GCAAAACAGG CCCTGTGGGT CCACAGGGAG TGCCAGGAAA ACCTGGAACA 
GAAGGCCTTA GAGGGCTTCC AGGATCAGTG GGTGAGCAAG GATCTCCAGG ACCTGCAGGA 
CAGAAAGGAC CACCTGGTCC TATTGGACCT CCCGGTTTAC CTGGTCTGCG TGGAGATTCT 
GGCGGCAAAG GAGAAAAGGG ACACCCGGGT CTTATCGGTC TCATTGGACC CCCAGGAGAA 
CAGGGTGAGA AAGGAGACAG AGGCATGCCT GGGCCCCATG GATCCAATGG ACCAAAAGGA 
GAGACTGGAA TGCCTGGAGG CACAGGACCT ATCGGCCCCT CTGGTCCTCC TGGTCTGCCT 
GGTCCTCAAG GTATTAAAGG AGCCAAAGGT TCTTCTGGTG GAGCTGGTCC AAAAGGAGAA 
AAAGGAGTAC AAGGACCCCC TGGACTTCCT GGCCCACCAG GGGAGGTTAT TCAGCCACTA 
CCCATCCAAA GGAGTCCCAA GTCCAAGCGG TCCATTGATG CCAGCCAGCT ACTTCCTGAG 
AATGACATGC CTGCATCCGA TGCCACCGGG ACAGAATTCC TGATGGGTAG TGAAGGCATG 
GAGGAGATCT TTGGCTCCCT CAACTCGCTT CGGCAGGAGA TTGAGACCAT GCGCTTCCCT 
CTGGGCACCC AGGACAGCCC CGGCCGCACC TGCCAAGACC TGCACCTCAG TCAGCCAGAA 
CTCAAAGATG GAGAATACTG GATTGACCCC AACCAGGGCT GCTCCAGAGA CGCCTTCAAG 
GTCTTCTGTA ACTTTACTAA GGGGGCAGAG ACGTGCCTCT ACCCCAGCCA GAGCATCAGC 
ACGGTGAAGA TGAATTCCTG GAACAAAGAG ACTCCAGGTT CCTGGTACAG TCAGTTCTCC 
ACTGGCAGTA AGTTTTCATA CGTGGACTCC AACGGCGAAC CTGTGGGTGT GGTTCAGTTG 
GGTTTCCTTC GTCTCTTGAG CGTGCAGGCC CACCAGACTC TCACATACCA CTGCCACCGC 
TCGGTAGCGT GGGCTGACCG CAACGCCAAG AACACATACA GCCGAGCTCT GCACTTCAGG 
GGTGCCAACG ACGAGGATCT AAGCTATGAG ACCAATCCAT ACGTCAAGGC TTTGGTTGAT 
GGCTGCTCTT ATCGTAAGGG CTTTGACCGG ACAGTCCTGG AGATCAACAC ACCACAGGTG 
GAACATCTTC CTCTGCTGGA TATCAAGGTG TCAGACTTTG GGGAGAGCAA CCAGCAGTTT 
GGCTTTGAAG TGGGACCTGT CTGTTTCCAA GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	LOC101066527
Entrez Gene ID	101066527
Full Name	collagen type XI alpha 2 chain
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC101066527 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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