ARSA cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARSA
Entrez Gene ID	396973
Full Name	arylsulfatase A
Synonyms	AS-A
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 5 Map Location: 5p15-p11

mRNA and Protein(s)

mRNA	Protein	Name
NM_213933.1	NP_999098.1	arylsulfatase A precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Interaction of arylsulfatase-A (ASA) with its natural sulfoglycolipid substrates: a computational and site-directed mutagenesis study.
Schenk M, Koppisetty CA, Santos DC, Carmona E, Bhatia S, Nyholm PG, Tanphaichitr N
Glycoconjugate journal26(8)1029-45(2009 Nov)

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Uenishi H, Eguchi-Ogawa T, Shinkai H, Okumura N, Suzuki K, Toki D, Hamasima N, Awata T
Nucleic acids research35(Database issue)D650-3(2007 Jan)

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Uenishi H, Eguchi T, Suzuki K, Sawazaki T, Toki D, Shinkai H, Okumura N, Hamasima N, Awata T
Nucleic acids research32(Database issue)D484-8(2004 Jan)

Arylsulfatase a is present on the pig sperm surface and is involved in sperm-zona pellucida binding.
Carmona E, Weerachatyanukul W, Soboloff T, Fluharty AL, White D, Promdee L, Ekker M, Berger T, Buhr M, Tanphaichitr N
Developmental biology247(1)182-96(2002 Jul)

Comparative porcine gene mapping relative to human chromosomes 9, 10, 20 and 22.
Lee JH, Zhang W, Moran C
Animal genetics32(5)313-5(2001 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cys69 was the active site residue, and Lys302 & Lys123 as residues anchoring the sulfate group of sulfogalactosylceramide/sulfogalactosylglycerolipid to the active site, as observed for nitrocatechol sulfate.
Title: Interaction of arylsulfatase-A (ASA) with its natural sulfoglycolipid substrates: a computational and site-directed mutagenesis study.

The following ARSA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARSA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe29528	NM_213933.1 Latest version!	Sus scrofa arylsulfatase A (ARSA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARSA ( NM_213933.1 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999098.1 Sus scrofa arylsulfatase A (ARSA), mRNA.

Price & Availability↑

CloneID	OSe29528
Clone ID Related Accession (Same CDS sequence)	NM_213933.1
Accession Version	NM_213933.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-17
Organism	Sus scrofa(pig)
Product	arylsulfatase A precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF316108.2. ##Evidence-Data-START## Transcript exon combination :: AF316108.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN01048301, SAMN01083986 [ECO:0000350] ##Evidence-Data-END##

ATGGTGGCTC TGTGGGCCCT CACTCTGGCC TTGGCCTCAG GCCTGGCTGC TACCAGCCCA 
CCTAACATCG TGCTGATCTT TGCTGATGAC CTGGGCTACG GAGACCTGGG CTCCTACGGG 
CACCCCAGTT CCACCACCCC CAATCTGGAC CAGCTGGCCG CAGGGGGTCT GCGGTTCACC 
GACTTCTATG TGCCGGTGTC TCTGTGCACA CCCTCCCGGG CTGCCCTCCT GACCGGCCGA 
CTCCCAGTTC GGATGGGCCT GTACCCTGGC GTTCTGGAAC CCAGCTCCCG AGGGGGTCTT 
CCCCTGGAGG AGGTGACCCT GGCTGAGGTC CTTGCTGCCC GAGGCTACCT CACAGGGATG 
GCTGGCAAGT GGCACCTTGG GGTGGGGCCT GAGGGGGCCT TTCTGCCCCC CCACCAGGGC 
TTCCATCGAT TCCTGGGCAT CCCATACTCC CATGACCAGG GCCCCTGCCA GAACCTGACC 
TGCTTCCCGC CGTCCACCCC CTGCGACGGC AGCTGTGACC AGGGCCTAGT CCCTGTCCCG 
CTATTGGCCA ACCTGTCGGT GGAGGCGCAG CCCCCTTGGC TGCCTGGACT TGAGGCCCGC 
TACGTGGCCT TCGCCCGTGA CCTCATGGCT GATGCCCAGC GCCAGGGCCG CCCATTCTTC 
CTGTACTACG CCTCCCACCA CACCCACTAC CCCCAGTTCA GTGGGCAGAG CTTTTCGGGG 
CACTCAGGCC GAGGGCCATT TGGGGACTCC CTGATGGAGC TGGATGCAGC TGTGGGGGCC 
CTGATGACAG CTGTGGGGGA CCTGGGGCTG CTCGGAGAGA CACTGGTCAT CTTCACTGCA 
GACAACGGAC CCGAGACGAT GCGGATGTCC CATGGCGGCT GCTCCGGCCT CCTACGATGC 
GGAAAGGGAA CCACTTTTGA GGGAGGTGTC CGAGAGCCTG CCTTGGCTTT CTGGCCAGGC 
CACATTGCTC CTGGTGTGAC CCACGAGCTG GCCAGCTCCC TAGACCTACT GCCCACCCTG 
GCGGCCCTGG CCGGGGCCCC GCTCCCCAAT GTCACCCTGG ATGGCGTTGA CCTCAGCCCC 
CTGCTGTTGG GTACAGGCAA GAGTCCCCGG CGGACGCTCT TCTTCTACCC GGCCTACCCA 
GATGAGGTCC GAGGGGTCTT TGCTGTGCGC AGCGGGAAGT ACAAGGCGCA TTTCTTCACC 
CAGGGCTCTA TCCACAGCGA CACCACTGCG GACCCTGCAT GCCACGCCTC TAGTCCTCTG 
ACTGCCCATG AGCCCCCGCT GCTCTTTGAC CTGTCTGAGG ACCCGGGTGA GAACTACAAC 
CTTCTGGGGG GTGTGGCTGA GGTTGCCCCA GAGGTGCTGC AGGTGCTGAA GCAACTTCAG 
CTGCTCAAGG CTCAGTTTGA TGCTGCCGTG ACCTTCAGCC CCAGCCAGAT AGCCCGGGGT 
GAGGACCCTG CCCTGCAGAT CTGCTGTCAA CCCAGCTGCA CCCCGAGGCC ATCCTGCTGC 
CACTGTCCTG AGCTCCAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999098.1
CDS	268..1791
Translation	MVALWALTLALASGLAATSPPNIVLIFADDLGYGDLGSYGHPSSTTPNLDQLAAGGLRFTDFYVPVSLCTPSRAALLTGRLPVRMGLYPGVLEPSSRGGLPLEEVTLAEVLAARGYLTGMAGKWHLGVGPEGAFLPPHQGFHRFLGIPYSHDQGPCQNLTCFPPSTPCDGSCDQGLVPVPLLANLSVEAQPPWLPGLEARYVAFARDLMADAQRQGRPFFLYYASHHTHYPQFSGQSFSGHSGRGPFGDSLMELDAAVGALMTAVGDLGLLGETLVIFTADNGPETMRMSHGGCSGLLRCGKGTTFEGGVREPALAFWPGHIAPGVTHELASSLDLLPTLAALAGAPLPNVTLDGVDLSPLLLGTGKSPRRTLFFYPAYPDEVRGVFAVRSGKYKAHFFTQGSIHSDTTADPACHASSPLTAHEPPLLFDLSEDPGENYNLLGGVAEVAPEVLQVLKQLQLLKAQFDAAVTFSPSQIARGEDPALQICCQPSCTPRPSCCHCPELQP

Target ORF information:

RefSeq Version	NM_213933.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa arylsulfatase A (ARSA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213933.1

ORF Insert Sequence:

ATGGTGGCTC TGTGGGCCCT CACTCTGGCC TTGGCCTCAG GCCTGGCTGC TACCAGCCCA 
CCTAACATCG TGCTGATCTT TGCTGATGAC CTGGGCTACG GAGACCTGGG CTCCTACGGG 
CACCCCAGTT CCACCACCCC CAATCTGGAC CAGCTGGCCG CAGGGGGTCT GCGGTTCACC 
GACTTCTATG TGCCGGTGTC TCTGTGCACA CCCTCCCGGG CTGCCCTCCT GACCGGCCGA 
CTCCCAGTTC GGATGGGCCT GTACCCTGGC GTTCTGGAAC CCAGCTCCCG AGGGGGTCTT 
CCCCTGGAGG AGGTGACCCT GGCTGAGGTC CTTGCTGCCC GAGGCTACCT CACAGGGATG 
GCTGGCAAGT GGCACCTTGG GGTGGGGCCT GAGGGGGCCT TTCTGCCCCC CCACCAGGGC 
TTCCATCGAT TCCTGGGCAT CCCATACTCC CATGACCAGG GCCCCTGCCA GAACCTGACC 
TGCTTCCCGC CGTCCACCCC CTGCGACGGC AGCTGTGACC AGGGCCTAGT CCCTGTCCCG 
CTATTGGCCA ACCTGTCGGT GGAGGCGCAG CCCCCTTGGC TGCCTGGACT TGAGGCCCGC 
TACGTGGCCT TCGCCCGTGA CCTCATGGCT GATGCCCAGC GCCAGGGCCG CCCATTCTTC 
CTGTACTACG CCTCCCACCA CACCCACTAC CCCCAGTTCA GTGGGCAGAG CTTTTCGGGG 
CACTCAGGCC GAGGGCCATT TGGGGACTCC CTGATGGAGC TGGATGCAGC TGTGGGGGCC 
CTGATGACAG CTGTGGGGGA CCTGGGGCTG CTCGGAGAGA CACTGGTCAT CTTCACTGCA 
GACAACGGAC CCGAGACGAT GCGGATGTCC CATGGCGGCT GCTCCGGCCT CCTACGATGC 
GGAAAGGGAA CCACTTTTGA GGGAGGTGTC CGAGAGCCTG CCTTGGCTTT CTGGCCAGGC 
CACATTGCTC CTGGTGTGAC CCACGAGCTG GCCAGCTCCC TAGACCTACT GCCCACCCTG 
GCGGCCCTGG CCGGGGCCCC GCTCCCCAAT GTCACCCTGG ATGGCGTTGA CCTCAGCCCC 
CTGCTGTTGG GTACAGGCAA GAGTCCCCGG CGGACGCTCT TCTTCTACCC GGCCTACCCA 
GATGAGGTCC GAGGGGTCTT TGCTGTGCGC AGCGGGAAGT ACAAGGCGCA TTTCTTCACC 
CAGGGCTCTA TCCACAGCGA CACCACTGCG GACCCTGCAT GCCACGCCTC TAGTCCTCTG 
ACTGCCCATG AGCCCCCGCT GCTCTTTGAC CTGTCTGAGG ACCCGGGTGA GAACTACAAC 
CTTCTGGGGG GTGTGGCTGA GGTTGCCCCA GAGGTGCTGC AGGTGCTGAA GCAACTTCAG 
CTGCTCAAGG CTCAGTTTGA TGCTGCCGTG ACCTTCAGCC CCAGCCAGAT AGCCCGGGGT 
GAGGACCCTG CCCTGCAGAT CTGCTGTCAA CCCAGCTGCA CCCCGAGGCC ATCCTGCTGC 
CACTGTCCTG AGCTCCAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interaction of arylsulfatase-A (ASA) with its natural sulfoglycolipid substrates: a computational and site-directed mutagenesis study.
Glycoconjugate journal26(8)1029-45(2009 Nov)
Schenk M,Koppisetty CA,Santos DC,Carmona E,Bhatia S,Nyholm PG,Tanphaichitr N

PEDE (Pig EST Data Explorer) has been expanded into Pig Expression Data Explorer, including 10 147 porcine full-length cDNA sequences.
Nucleic acids research35(Database issue)D650-3(2007 Jan)
Uenishi H,Eguchi-Ogawa T,Shinkai H,Okumura N,Suzuki K,Toki D,Hamasima N,Awata T

PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries.
Nucleic acids research32(Database issue)D484-8(2004 Jan)
Uenishi H,Eguchi T,Suzuki K,Sawazaki T,Toki D,Shinkai H,Okumura N,Hamasima N,Awata T

Arylsulfatase a is present on the pig sperm surface and is involved in sperm-zona pellucida binding.
Developmental biology247(1)182-96(2002 Jul)
Carmona E,Weerachatyanukul W,Soboloff T,Fluharty AL,White D,Promdee L,Ekker M,Berger T,Buhr M,Tanphaichitr N

Comparative porcine gene mapping relative to human chromosomes 9, 10, 20 and 22.
Animal genetics32(5)313-5(2001 Oct)
Lee JH,Zhang W,Moran C

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ARSA cDNA ORF clone, Sus scrofa(pig)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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