DHX30 cDNA ORF clone, Struthio camelus australis

Gene
Clones
gRNAs

Summary

Gene Symbol	DHX30
Entrez Gene ID	104150101
Full Name	DExH-box helicase 30
Gene Type	protein-coding
Organism	Struthio camelus australis

mRNA and Protein(s)

mRNA	Protein	Name
XM_009684122.1	XP_009682417.1	putative ATP-dependent RNA helicase DHX30 isoform X1
XM_009684123.1	XP_009682418.1	putative ATP-dependent RNA helicase DHX30 isoform X2

Pathways from BioSystems
Gene Ontology

The following DHX30 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHX30 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSt79959	XM_009684122.1 Latest version!	Struthio camelus australis DEAH (Asp-Glu-Ala-His) box helicase 30 (DHX30), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OSt79960	XM_009684123.1 Latest version!	Struthio camelus australis DEAH (Asp-Glu-Ala-His) box helicase 30 (DHX30), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DHX30 ( XM_009684122.1 ) cDNA ORF clone, Struthio camelus australis -> XP_009682417.1 Struthio camelus australis DEAH (Asp-Glu-Ala-His) box helicase 30 (DHX30), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSt79959
Clone ID Related Accession (Same CDS sequence)	XM_009684122.1
Accession Version	XM_009684122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-14
Organism	Struthio camelus australis
Product	putative ATP-dependent RNA helicase DHX30 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009271904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Struthio camelus australis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATGT TCTCGTTGGA TTCCCTTAAA GATTCCAGAG ACTTGCTAAA GGAATTTCCA 
CAGCCTAAAA ACTTGCTCAA CAGCGTGATT GGACGAGCCC TGGGTATTTC TCATGCAAGG 
GACAAACTGG TATACATCCA TACTAATGGG CCACGGAAAA AGAAAGTCAC TCTGCATATA 
AAGTGGCCTA AGAATGTGGA AGTGGAAGGC TATGGGACCA AGAAGATTGA TGCTGAAAGG 
CAGGCAGCAG CTGCTGCATG TCAGCTCTTC AAGGGCTGGG GTTTGCTGGG GCCCAGAAAT 
GAGCTGTTTG ATGCTGCAAA GTACCGGCTT CTGGCTGACC AGCTTGGCTG TCCTGATGAG 
AGGTGGTGCT CCGAGGGCAA GTGGCGCTCC AAGTCCGGAC CTTCTCTCGC GGACTTGTCA 
ACCTGCTGGC GACGGATGGA GCCGGATGAT TCCATCCAGC CCATGGAGCA AGGCAGGATG 
CCTAAAGCAA TGAGGAGAGA AGAGCTGGAA GAAGGGGAGC TAGAGGAAGG CGAACTAGAG 
GAAGGGGAGC TGGAAGAAGA AGCAATTGAT GTTTCTGATT ATCTACCTAT GGCACATCAG 
GATGCTCGAA CCCCAGGCAG AGATGCGAGC AGGGGAGGGA GTTCCATTGA AATGACAGAT 
GACAACACTG CCATCCGCGC TCTGACTCAG TTCCCGCTTC CCAAAAACCT TCTGGCCCAA 
GTGATTCAGA TTGCGACCTC TTCTTCTACA GTCAAGGAAT ACATGCAGTT CCGTACAGTC 
GGCACCAAGA CCAAGATCTG CAAGCTCACG CTTCGCTGGC CCTGTCCAAT GACTTTTGCT 
GCCAAGGGCC GTCGCAAGGT GGAGGCAGAA AACAAGGCAG CGGCACTGGC TTGTCAGAAG 
CTTAAGAGCC TTGGCTTAGT GGACAAGAAC AACAACCCAC TCAGCCATGC TATGTACAAC 
ATGACCTCCC TCCGAGAGCT TGGTGAGAAC CAGCGGAAAC CCTGCCACAT CAAAGTCCCT 
GAAGCCACCC TCCGCAAGAT TGAGAACTAT CTGAATCATT ATCCAGTGGA CATTAGGGAA 
TCCAGGCCCC GGATTGCTGA TGACATGATG AACCTGAGCA AGGAGACTGG TGCGATAAGC 
GATGCGATCA CGGGGAAGAC GTATATACCC ATGTTGGAAG CCGAGGAAGT GCGCCTTAGT 
CAGAATCTCC TGGCCCTCTG GAAGAGGAGA GGAGCCTCGT GGCAGGAGAG CCACCCGCTG 
CCAGTGGATC CTCACAAGGA CACCATCCTA TCAGCCATTG AGCAGAATCC TGTAGTGGTA 
ATAGCAGGAG ACACAGGCTG TGGGAAAACC ACACGGATTC CTCAGCTCCT GCTGGAGCAC 
TACATCCTGG AGGGCCGTGG TGCCCGTTGC AACGTAGTGA TCACCCAGCC GAGGAGGATC 
AGCGCCATCT CGGTTGCCCA GCGCGTGGCA CAAGAATTGG GTCCCAACAT GAGGAAGAAT 
GTGGGCTACC AGGTGCGACT GGAGAGCAAG CCGCCCGCCA GGGGAGGTGC TCTGCTCTTC 
TGCACAGTGG GCATCCTGCT GCGGAAGCTG CAGGGGAATC CCAGCCTGGA GGGTGTCAGC 
CACGTTGACA GGTATTCCCT GCTAACCCTG CTGAAAGGCA TCCAGAAGCT CAACCCTGAC 
CTGCGCCTGG TTTTGATGAG CGCCACAGGA GACAACCAGC GCTTCTCGCA CTACTTTGGG 
GATTGCCCTG TGGTCAAGGT GCCGGGCTTC ATGTATCCAG TGAAGGAGTA CTACCTGGAG 
GAGATCTTGG CCAAGCTGGG CCGGCACCGA CACCGGCACT ATGAGATCAA GCAATCGGAT 
GATGAATGTG TCCTTGACCT TGATCTGATC ACTGACCTTG TACTCCAGAT TGACGCCCAT 
GGAGAACCAG GCGGGATCCT CTGCTTTCTT CCTGGTTGGC AGGAAATCAA AGGGGTGCAG 
CAGCGCCTGC TAGAGATGCT TGGATCTCAG AACAGCCGTT ACCTTGTCTT ACCAGTGCAC 
TCCAACATCC CCATGATGGA CCAGCAAAAC ATTTTCCAGC GGCCTCCGCC TGGTGTCAGA 
AAGATTGTCC TGGCCACCAA CATCGCTGAG ACCTCCATCA CCATCAACGA CATTGTCCAC 
GTGGTGGACA GTGGCACGCA TAAGGAGGAA CGCTATGACC TAAAGACCAA GGTGTCCTGC 
CTGGAAACAG TGTGGGTGTC CAAGTCAAAT GTCGTACAGC GGCGAGGGCG TGCTGGCCGC 
TGTCAGTCGG GATTTGCCTA CCACCTCTTC CCTCGCAGCC GCCTGGACAA GATGCCAACT 
TACCAGGTTC CAGAGATCCT TCGCACCCCA CTGGAGAATC TGGTGGTTCA GGCCAAGATC 
CACATGCCAG AGAAAACAGC AGTTGAATTT CTCTCTAAGG CTCTGGACAG CCCTGACATT 
AAAGCTGTGG ATGAAGCAGT GATCTTGCTG CAGGAGATTG GAGTGCTGGA CCAGCGAGAA 
GCCCTCACCA CTCTAGGCAA ACGCCTTGCC CAGATCTCCA CAGACCCTCG GCTGGCAAAG 
GCCATCGTCT TGGCGTCCAT CTATCGCTGC CTCCACCCTC TGCTTGTCAT CGTTTCCTGC 
CTCACTCGGG ACCCCTTCAG CAGCAGCCTG CAAAACCGTG CAGAGGTGGA CAAGGCCAAG 
GCTGTTCTAA GCCGGGAGAG CGGGAGTGAT CACCTGGCGT TTGTCAGAGC TGTGGCAGGC 
TGGGAGGAGG TGCTCAGGCG CAGAGACAGT CGCGCCAGAG ATAACTACCT GCAGGACTAT 
TATCTCTATG GCCCCAGCCT TCGCTTCATC AATGGCCTTG TCAAGCAGTT CTCTGAGAAC 
CTCTATGAAG CCTTCCTGGT GTCATCCCCA TCTGACTGTA CCATGCCCTC GTCTGTGTGT 
AACCAGTACA GTGAAGAGGA AGAACTGGTC AAAGGAGTCC TTATGGCTGG GCTCTATCCC 
AACCTCATCC AGGTGAGACA AGGCAAGGTG ACCCGTCAGG GGAAGTTCAA ACCCAACAGC 
TACGCGTATC GGACAAAGGC TGGCACCGTT CTGCTCCACA AGTCTACAAT CAACAGGGAG 
GCATCCAAGC TGTACAGTCG CTGGCTAACG TACTTCATGG CGGTGAAGTC CAACGGTGGG 
GTGTTTGTAC GGGACTCCTC CCAGGTCCAC CCGCTGGCTG TACTGCTCAT GACTGACACA 
GATATCCATG TCCGAGATGA TGGCTGGCGA GCAACAGTCT CCCTGACAGA CAGTGATCTC 
CTGGTGCTGG AGGGAGACTC CTACACCATC CGCCTGCTGC GTGATTTCCG CGTGTCACTC 
TCCAAGATGG TGGAGACGTG CCTGTGCTAT GAGATGGCAG CCATCCCTGG GGACCTGCAC 
CACCAGCACA GCCAGCTGCT TGACATCCTG GTGGATCTGC TCAAAGGCCC TCCTGGCAGC 
TTTGGTGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009682417.1
CDS	22..3513
Translation	MEMFSLDSLKDSRDLLKEFPQPKNLLNSVIGRALGISHARDKLVYIHTNGPRKKKVTLHIKWPKNVEVEGYGTKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRLLADQLGCPDERWCSEGKWRSKSGPSLADLSTCWRRMEPDDSIQPMEQGRMPKAMRREELEEGELEEGELEEGELEEEAIDVSDYLPMAHQDARTPGRDASRGGSSIEMTDDNTAIRALTQFPLPKNLLAQVIQIATSSSTVKEYMQFRTVGTKTKICKLTLRWPCPMTFAAKGRRKVEAENKAAALACQKLKSLGLVDKNNNPLSHAMYNMTSLRELGENQRKPCHIKVPEATLRKIENYLNHYPVDIRESRPRIADDMMNLSKETGAISDAITGKTYIPMLEAEEVRLSQNLLALWKRRGASWQESHPLPVDPHKDTILSAIEQNPVVVIAGDTGCGKTTRIPQLLLEHYILEGRGARCNVVITQPRRISAISVAQRVAQELGPNMRKNVGYQVRLESKPPARGGALLFCTVGILLRKLQGNPSLEGVSHVDRYSLLTLLKGIQKLNPDLRLVLMSATGDNQRFSHYFGDCPVVKVPGFMYPVKEYYLEEILAKLGRHRHRHYEIKQSDDECVLDLDLITDLVLQIDAHGEPGGILCFLPGWQEIKGVQQRLLEMLGSQNSRYLVLPVHSNIPMMDQQNIFQRPPPGVRKIVLATNIAETSITINDIVHVVDSGTHKEERYDLKTKVSCLETVWVSKSNVVQRRGRAGRCQSGFAYHLFPRSRLDKMPTYQVPEILRTPLENLVVQAKIHMPEKTAVEFLSKALDSPDIKAVDEAVILLQEIGVLDQREALTTLGKRLAQISTDPRLAKAIVLASIYRCLHPLLVIVSCLTRDPFSSSLQNRAEVDKAKAVLSRESGSDHLAFVRAVAGWEEVLRRRDSRARDNYLQDYYLYGPSLRFINGLVKQFSENLYEAFLVSSPSDCTMPSSVCNQYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSYAYRTKAGTVLLHKSTINREASKLYSRWLTYFMAVKSNGGVFVRDSSQVHPLAVLLMTDTDIHVRDDGWRATVSLTDSDLLVLEGDSYTIRLLRDFRVSLSKMVETCLCYEMAAIPGDLHHQHSQLLDILVDLLKGPPGSFGA

Target ORF information:

RefSeq Version	XM_009684122.1
Organism	Struthio camelus australis
Definition	Struthio camelus australis DEAH (Asp-Glu-Ala-His) box helicase 30 (DHX30), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009684122.1

ORF Insert Sequence:

ATGGAGATGT TCTCGTTGGA TTCCCTTAAA GATTCCAGAG ACTTGCTAAA GGAATTTCCA 
CAGCCTAAAA ACTTGCTCAA CAGCGTGATT GGACGAGCCC TGGGTATTTC TCATGCAAGG 
GACAAACTGG TATACATCCA TACTAATGGG CCACGGAAAA AGAAAGTCAC TCTGCATATA 
AAGTGGCCTA AGAATGTGGA AGTGGAAGGC TATGGGACCA AGAAGATTGA TGCTGAAAGG 
CAGGCAGCAG CTGCTGCATG TCAGCTCTTC AAGGGCTGGG GTTTGCTGGG GCCCAGAAAT 
GAGCTGTTTG ATGCTGCAAA GTACCGGCTT CTGGCTGACC AGCTTGGCTG TCCTGATGAG 
AGGTGGTGCT CCGAGGGCAA GTGGCGCTCC AAGTCCGGAC CTTCTCTCGC GGACTTGTCA 
ACCTGCTGGC GACGGATGGA GCCGGATGAT TCCATCCAGC CCATGGAGCA AGGCAGGATG 
CCTAAAGCAA TGAGGAGAGA AGAGCTGGAA GAAGGGGAGC TAGAGGAAGG CGAACTAGAG 
GAAGGGGAGC TGGAAGAAGA AGCAATTGAT GTTTCTGATT ATCTACCTAT GGCACATCAG 
GATGCTCGAA CCCCAGGCAG AGATGCGAGC AGGGGAGGGA GTTCCATTGA AATGACAGAT 
GACAACACTG CCATCCGCGC TCTGACTCAG TTCCCGCTTC CCAAAAACCT TCTGGCCCAA 
GTGATTCAGA TTGCGACCTC TTCTTCTACA GTCAAGGAAT ACATGCAGTT CCGTACAGTC 
GGCACCAAGA CCAAGATCTG CAAGCTCACG CTTCGCTGGC CCTGTCCAAT GACTTTTGCT 
GCCAAGGGCC GTCGCAAGGT GGAGGCAGAA AACAAGGCAG CGGCACTGGC TTGTCAGAAG 
CTTAAGAGCC TTGGCTTAGT GGACAAGAAC AACAACCCAC TCAGCCATGC TATGTACAAC 
ATGACCTCCC TCCGAGAGCT TGGTGAGAAC CAGCGGAAAC CCTGCCACAT CAAAGTCCCT 
GAAGCCACCC TCCGCAAGAT TGAGAACTAT CTGAATCATT ATCCAGTGGA CATTAGGGAA 
TCCAGGCCCC GGATTGCTGA TGACATGATG AACCTGAGCA AGGAGACTGG TGCGATAAGC 
GATGCGATCA CGGGGAAGAC GTATATACCC ATGTTGGAAG CCGAGGAAGT GCGCCTTAGT 
CAGAATCTCC TGGCCCTCTG GAAGAGGAGA GGAGCCTCGT GGCAGGAGAG CCACCCGCTG 
CCAGTGGATC CTCACAAGGA CACCATCCTA TCAGCCATTG AGCAGAATCC TGTAGTGGTA 
ATAGCAGGAG ACACAGGCTG TGGGAAAACC ACACGGATTC CTCAGCTCCT GCTGGAGCAC 
TACATCCTGG AGGGCCGTGG TGCCCGTTGC AACGTAGTGA TCACCCAGCC GAGGAGGATC 
AGCGCCATCT CGGTTGCCCA GCGCGTGGCA CAAGAATTGG GTCCCAACAT GAGGAAGAAT 
GTGGGCTACC AGGTGCGACT GGAGAGCAAG CCGCCCGCCA GGGGAGGTGC TCTGCTCTTC 
TGCACAGTGG GCATCCTGCT GCGGAAGCTG CAGGGGAATC CCAGCCTGGA GGGTGTCAGC 
CACGTTGACA GGTATTCCCT GCTAACCCTG CTGAAAGGCA TCCAGAAGCT CAACCCTGAC 
CTGCGCCTGG TTTTGATGAG CGCCACAGGA GACAACCAGC GCTTCTCGCA CTACTTTGGG 
GATTGCCCTG TGGTCAAGGT GCCGGGCTTC ATGTATCCAG TGAAGGAGTA CTACCTGGAG 
GAGATCTTGG CCAAGCTGGG CCGGCACCGA CACCGGCACT ATGAGATCAA GCAATCGGAT 
GATGAATGTG TCCTTGACCT TGATCTGATC ACTGACCTTG TACTCCAGAT TGACGCCCAT 
GGAGAACCAG GCGGGATCCT CTGCTTTCTT CCTGGTTGGC AGGAAATCAA AGGGGTGCAG 
CAGCGCCTGC TAGAGATGCT TGGATCTCAG AACAGCCGTT ACCTTGTCTT ACCAGTGCAC 
TCCAACATCC CCATGATGGA CCAGCAAAAC ATTTTCCAGC GGCCTCCGCC TGGTGTCAGA 
AAGATTGTCC TGGCCACCAA CATCGCTGAG ACCTCCATCA CCATCAACGA CATTGTCCAC 
GTGGTGGACA GTGGCACGCA TAAGGAGGAA CGCTATGACC TAAAGACCAA GGTGTCCTGC 
CTGGAAACAG TGTGGGTGTC CAAGTCAAAT GTCGTACAGC GGCGAGGGCG TGCTGGCCGC 
TGTCAGTCGG GATTTGCCTA CCACCTCTTC CCTCGCAGCC GCCTGGACAA GATGCCAACT 
TACCAGGTTC CAGAGATCCT TCGCACCCCA CTGGAGAATC TGGTGGTTCA GGCCAAGATC 
CACATGCCAG AGAAAACAGC AGTTGAATTT CTCTCTAAGG CTCTGGACAG CCCTGACATT 
AAAGCTGTGG ATGAAGCAGT GATCTTGCTG CAGGAGATTG GAGTGCTGGA CCAGCGAGAA 
GCCCTCACCA CTCTAGGCAA ACGCCTTGCC CAGATCTCCA CAGACCCTCG GCTGGCAAAG 
GCCATCGTCT TGGCGTCCAT CTATCGCTGC CTCCACCCTC TGCTTGTCAT CGTTTCCTGC 
CTCACTCGGG ACCCCTTCAG CAGCAGCCTG CAAAACCGTG CAGAGGTGGA CAAGGCCAAG 
GCTGTTCTAA GCCGGGAGAG CGGGAGTGAT CACCTGGCGT TTGTCAGAGC TGTGGCAGGC 
TGGGAGGAGG TGCTCAGGCG CAGAGACAGT CGCGCCAGAG ATAACTACCT GCAGGACTAT 
TATCTCTATG GCCCCAGCCT TCGCTTCATC AATGGCCTTG TCAAGCAGTT CTCTGAGAAC 
CTCTATGAAG CCTTCCTGGT GTCATCCCCA TCTGACTGTA CCATGCCCTC GTCTGTGTGT 
AACCAGTACA GTGAAGAGGA AGAACTGGTC AAAGGAGTCC TTATGGCTGG GCTCTATCCC 
AACCTCATCC AGGTGAGACA AGGCAAGGTG ACCCGTCAGG GGAAGTTCAA ACCCAACAGC 
TACGCGTATC GGACAAAGGC TGGCACCGTT CTGCTCCACA AGTCTACAAT CAACAGGGAG 
GCATCCAAGC TGTACAGTCG CTGGCTAACG TACTTCATGG CGGTGAAGTC CAACGGTGGG 
GTGTTTGTAC GGGACTCCTC CCAGGTCCAC CCGCTGGCTG TACTGCTCAT GACTGACACA 
GATATCCATG TCCGAGATGA TGGCTGGCGA GCAACAGTCT CCCTGACAGA CAGTGATCTC 
CTGGTGCTGG AGGGAGACTC CTACACCATC CGCCTGCTGC GTGATTTCCG CGTGTCACTC 
TCCAAGATGG TGGAGACGTG CCTGTGCTAT GAGATGGCAG CCATCCCTGG GGACCTGCAC 
CACCAGCACA GCCAGCTGCT TGACATCCTG GTGGATCTGC TCAAAGGCCC TCCTGGCAGC 
TTTGGTGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DHX30 ( XM_009684123.1 ) cDNA ORF clone, Struthio camelus australis -> XP_009682418.1 Struthio camelus australis DEAH (Asp-Glu-Ala-His) box helicase 30 (DHX30), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSt79960
Clone ID Related Accession (Same CDS sequence)	XM_009684123.1
Accession Version	XM_009684123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-14
Organism	Struthio camelus australis
Product	putative ATP-dependent RNA helicase DHX30 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009271904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Struthio camelus australis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGATGT TCTCGTTGGA TTCCCTTAAA GATTCCAGAG ACTTGCTAAA GGAATTTCCA 
CAGCCTAAAA ACTTGCTCAA CAGCGTGATT GGACGAGCCC TGGGTATTTC TCATGCAAGG 
GACAAACTGG TATACATCCA TACTAATGGG CCACGGAAAA AGAAAGTCAC TCTGCATATA 
AAGTGGCCTA AGAATGTGGA AGTGGAAGGC TATGGGACCA AGAAGATTGA TGCTGAAAGG 
CAGGCAGCAG CTGCTGCATG TCAGCTCTTC AAGGGCTGGG GTTTGCTGGG GCCCAGAAAT 
GAGCTGTTTG ATGCTGCAAA GTACCGGCTT CTGGCTGACC AGCTTGGCTG TCCTGATGAG 
AGGTGGTGCT CCGAGGGCAA GTGGCGCTCC AAGTCCGGAC CTTCTCTCGC GGACTTGTCA 
ACCTGCTGGC GACGGATGGA GCCGGATGAT TCCATCCAGC CCATGGAGCA AGGCAGGATG 
CCTAAAGCAA TGAGGAGAGA AGAGCTGGAA GAAGGGGAGC TAGAGGAAGG CGAACTAGAG 
GAAGGGGAGC TGGAAGAAGA AGCAATTGAT GTTTCTGATT ATCTACCTAT GGCACATCAG 
GATGCTCGAA CCCCAGGCAG AGATGCGAGG GGAGGGAGTT CCATTGAAAT GACAGATGAC 
AACACTGCCA TCCGCGCTCT GACTCAGTTC CCGCTTCCCA AAAACCTTCT GGCCCAAGTG 
ATTCAGATTG CGACCTCTTC TTCTACAGTC AAGGAATACA TGCAGTTCCG TACAGTCGGC 
ACCAAGACCA AGATCTGCAA GCTCACGCTT CGCTGGCCCT GTCCAATGAC TTTTGCTGCC 
AAGGGCCGTC GCAAGGTGGA GGCAGAAAAC AAGGCAGCGG CACTGGCTTG TCAGAAGCTT 
AAGAGCCTTG GCTTAGTGGA CAAGAACAAC AACCCACTCA GCCATGCTAT GTACAACATG 
ACCTCCCTCC GAGAGCTTGG TGAGAACCAG CGGAAACCCT GCCACATCAA AGTCCCTGAA 
GCCACCCTCC GCAAGATTGA GAACTATCTG AATCATTATC CAGTGGACAT TAGGGAATCC 
AGGCCCCGGA TTGCTGATGA CATGATGAAC CTGAGCAAGG AGACTGGTGC GATAAGCGAT 
GCGATCACGG GGAAGACGTA TATACCCATG TTGGAAGCCG AGGAAGTGCG CCTTAGTCAG 
AATCTCCTGG CCCTCTGGAA GAGGAGAGGA GCCTCGTGGC AGGAGAGCCA CCCGCTGCCA 
GTGGATCCTC ACAAGGACAC CATCCTATCA GCCATTGAGC AGAATCCTGT AGTGGTAATA 
GCAGGAGACA CAGGCTGTGG GAAAACCACA CGGATTCCTC AGCTCCTGCT GGAGCACTAC 
ATCCTGGAGG GCCGTGGTGC CCGTTGCAAC GTAGTGATCA CCCAGCCGAG GAGGATCAGC 
GCCATCTCGG TTGCCCAGCG CGTGGCACAA GAATTGGGTC CCAACATGAG GAAGAATGTG 
GGCTACCAGG TGCGACTGGA GAGCAAGCCG CCCGCCAGGG GAGGTGCTCT GCTCTTCTGC 
ACAGTGGGCA TCCTGCTGCG GAAGCTGCAG GGGAATCCCA GCCTGGAGGG TGTCAGCCAC 
GTTGACAGGT ATTCCCTGCT AACCCTGCTG AAAGGCATCC AGAAGCTCAA CCCTGACCTG 
CGCCTGGTTT TGATGAGCGC CACAGGAGAC AACCAGCGCT TCTCGCACTA CTTTGGGGAT 
TGCCCTGTGG TCAAGGTGCC GGGCTTCATG TATCCAGTGA AGGAGTACTA CCTGGAGGAG 
ATCTTGGCCA AGCTGGGCCG GCACCGACAC CGGCACTATG AGATCAAGCA ATCGGATGAT 
GAATGTGTCC TTGACCTTGA TCTGATCACT GACCTTGTAC TCCAGATTGA CGCCCATGGA 
GAACCAGGCG GGATCCTCTG CTTTCTTCCT GGTTGGCAGG AAATCAAAGG GGTGCAGCAG 
CGCCTGCTAG AGATGCTTGG ATCTCAGAAC AGCCGTTACC TTGTCTTACC AGTGCACTCC 
AACATCCCCA TGATGGACCA GCAAAACATT TTCCAGCGGC CTCCGCCTGG TGTCAGAAAG 
ATTGTCCTGG CCACCAACAT CGCTGAGACC TCCATCACCA TCAACGACAT TGTCCACGTG 
GTGGACAGTG GCACGCATAA GGAGGAACGC TATGACCTAA AGACCAAGGT GTCCTGCCTG 
GAAACAGTGT GGGTGTCCAA GTCAAATGTC GTACAGCGGC GAGGGCGTGC TGGCCGCTGT 
CAGTCGGGAT TTGCCTACCA CCTCTTCCCT CGCAGCCGCC TGGACAAGAT GCCAACTTAC 
CAGGTTCCAG AGATCCTTCG CACCCCACTG GAGAATCTGG TGGTTCAGGC CAAGATCCAC 
ATGCCAGAGA AAACAGCAGT TGAATTTCTC TCTAAGGCTC TGGACAGCCC TGACATTAAA 
GCTGTGGATG AAGCAGTGAT CTTGCTGCAG GAGATTGGAG TGCTGGACCA GCGAGAAGCC 
CTCACCACTC TAGGCAAACG CCTTGCCCAG ATCTCCACAG ACCCTCGGCT GGCAAAGGCC 
ATCGTCTTGG CGTCCATCTA TCGCTGCCTC CACCCTCTGC TTGTCATCGT TTCCTGCCTC 
ACTCGGGACC CCTTCAGCAG CAGCCTGCAA AACCGTGCAG AGGTGGACAA GGCCAAGGCT 
GTTCTAAGCC GGGAGAGCGG GAGTGATCAC CTGGCGTTTG TCAGAGCTGT GGCAGGCTGG 
GAGGAGGTGC TCAGGCGCAG AGACAGTCGC GCCAGAGATA ACTACCTGCA GGACTATTAT 
CTCTATGGCC CCAGCCTTCG CTTCATCAAT GGCCTTGTCA AGCAGTTCTC TGAGAACCTC 
TATGAAGCCT TCCTGGTGTC ATCCCCATCT GACTGTACCA TGCCCTCGTC TGTGTGTAAC 
CAGTACAGTG AAGAGGAAGA ACTGGTCAAA GGAGTCCTTA TGGCTGGGCT CTATCCCAAC 
CTCATCCAGG TGAGACAAGG CAAGGTGACC CGTCAGGGGA AGTTCAAACC CAACAGCTAC 
GCGTATCGGA CAAAGGCTGG CACCGTTCTG CTCCACAAGT CTACAATCAA CAGGGAGGCA 
TCCAAGCTGT ACAGTCGCTG GCTAACGTAC TTCATGGCGG TGAAGTCCAA CGGTGGGGTG 
TTTGTACGGG ACTCCTCCCA GGTCCACCCG CTGGCTGTAC TGCTCATGAC TGACACAGAT 
ATCCATGTCC GAGATGATGG CTGGCGAGCA ACAGTCTCCC TGACAGACAG TGATCTCCTG 
GTGCTGGAGG GAGACTCCTA CACCATCCGC CTGCTGCGTG ATTTCCGCGT GTCACTCTCC 
AAGATGGTGG AGACGTGCCT GTGCTATGAG ATGGCAGCCA TCCCTGGGGA CCTGCACCAC 
CAGCACAGCC AGCTGCTTGA CATCCTGGTG GATCTGCTCA AAGGCCCTCC TGGCAGCTTT 
GGTGCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009682418.1
CDS	22..3510
Translation	MEMFSLDSLKDSRDLLKEFPQPKNLLNSVIGRALGISHARDKLVYIHTNGPRKKKVTLHIKWPKNVEVEGYGTKKIDAERQAAAAACQLFKGWGLLGPRNELFDAAKYRLLADQLGCPDERWCSEGKWRSKSGPSLADLSTCWRRMEPDDSIQPMEQGRMPKAMRREELEEGELEEGELEEGELEEEAIDVSDYLPMAHQDARTPGRDARGGSSIEMTDDNTAIRALTQFPLPKNLLAQVIQIATSSSTVKEYMQFRTVGTKTKICKLTLRWPCPMTFAAKGRRKVEAENKAAALACQKLKSLGLVDKNNNPLSHAMYNMTSLRELGENQRKPCHIKVPEATLRKIENYLNHYPVDIRESRPRIADDMMNLSKETGAISDAITGKTYIPMLEAEEVRLSQNLLALWKRRGASWQESHPLPVDPHKDTILSAIEQNPVVVIAGDTGCGKTTRIPQLLLEHYILEGRGARCNVVITQPRRISAISVAQRVAQELGPNMRKNVGYQVRLESKPPARGGALLFCTVGILLRKLQGNPSLEGVSHVDRYSLLTLLKGIQKLNPDLRLVLMSATGDNQRFSHYFGDCPVVKVPGFMYPVKEYYLEEILAKLGRHRHRHYEIKQSDDECVLDLDLITDLVLQIDAHGEPGGILCFLPGWQEIKGVQQRLLEMLGSQNSRYLVLPVHSNIPMMDQQNIFQRPPPGVRKIVLATNIAETSITINDIVHVVDSGTHKEERYDLKTKVSCLETVWVSKSNVVQRRGRAGRCQSGFAYHLFPRSRLDKMPTYQVPEILRTPLENLVVQAKIHMPEKTAVEFLSKALDSPDIKAVDEAVILLQEIGVLDQREALTTLGKRLAQISTDPRLAKAIVLASIYRCLHPLLVIVSCLTRDPFSSSLQNRAEVDKAKAVLSRESGSDHLAFVRAVAGWEEVLRRRDSRARDNYLQDYYLYGPSLRFINGLVKQFSENLYEAFLVSSPSDCTMPSSVCNQYSEEEELVKGVLMAGLYPNLIQVRQGKVTRQGKFKPNSYAYRTKAGTVLLHKSTINREASKLYSRWLTYFMAVKSNGGVFVRDSSQVHPLAVLLMTDTDIHVRDDGWRATVSLTDSDLLVLEGDSYTIRLLRDFRVSLSKMVETCLCYEMAAIPGDLHHQHSQLLDILVDLLKGPPGSFGA

Target ORF information:

RefSeq Version	XM_009684123.1
Organism	Struthio camelus australis
Definition	Struthio camelus australis DEAH (Asp-Glu-Ala-His) box helicase 30 (DHX30), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009684123.1

ORF Insert Sequence:

ATGGAGATGT TCTCGTTGGA TTCCCTTAAA GATTCCAGAG ACTTGCTAAA GGAATTTCCA 
CAGCCTAAAA ACTTGCTCAA CAGCGTGATT GGACGAGCCC TGGGTATTTC TCATGCAAGG 
GACAAACTGG TATACATCCA TACTAATGGG CCACGGAAAA AGAAAGTCAC TCTGCATATA 
AAGTGGCCTA AGAATGTGGA AGTGGAAGGC TATGGGACCA AGAAGATTGA TGCTGAAAGG 
CAGGCAGCAG CTGCTGCATG TCAGCTCTTC AAGGGCTGGG GTTTGCTGGG GCCCAGAAAT 
GAGCTGTTTG ATGCTGCAAA GTACCGGCTT CTGGCTGACC AGCTTGGCTG TCCTGATGAG 
AGGTGGTGCT CCGAGGGCAA GTGGCGCTCC AAGTCCGGAC CTTCTCTCGC GGACTTGTCA 
ACCTGCTGGC GACGGATGGA GCCGGATGAT TCCATCCAGC CCATGGAGCA AGGCAGGATG 
CCTAAAGCAA TGAGGAGAGA AGAGCTGGAA GAAGGGGAGC TAGAGGAAGG CGAACTAGAG 
GAAGGGGAGC TGGAAGAAGA AGCAATTGAT GTTTCTGATT ATCTACCTAT GGCACATCAG 
GATGCTCGAA CCCCAGGCAG AGATGCGAGG GGAGGGAGTT CCATTGAAAT GACAGATGAC 
AACACTGCCA TCCGCGCTCT GACTCAGTTC CCGCTTCCCA AAAACCTTCT GGCCCAAGTG 
ATTCAGATTG CGACCTCTTC TTCTACAGTC AAGGAATACA TGCAGTTCCG TACAGTCGGC 
ACCAAGACCA AGATCTGCAA GCTCACGCTT CGCTGGCCCT GTCCAATGAC TTTTGCTGCC 
AAGGGCCGTC GCAAGGTGGA GGCAGAAAAC AAGGCAGCGG CACTGGCTTG TCAGAAGCTT 
AAGAGCCTTG GCTTAGTGGA CAAGAACAAC AACCCACTCA GCCATGCTAT GTACAACATG 
ACCTCCCTCC GAGAGCTTGG TGAGAACCAG CGGAAACCCT GCCACATCAA AGTCCCTGAA 
GCCACCCTCC GCAAGATTGA GAACTATCTG AATCATTATC CAGTGGACAT TAGGGAATCC 
AGGCCCCGGA TTGCTGATGA CATGATGAAC CTGAGCAAGG AGACTGGTGC GATAAGCGAT 
GCGATCACGG GGAAGACGTA TATACCCATG TTGGAAGCCG AGGAAGTGCG CCTTAGTCAG 
AATCTCCTGG CCCTCTGGAA GAGGAGAGGA GCCTCGTGGC AGGAGAGCCA CCCGCTGCCA 
GTGGATCCTC ACAAGGACAC CATCCTATCA GCCATTGAGC AGAATCCTGT AGTGGTAATA 
GCAGGAGACA CAGGCTGTGG GAAAACCACA CGGATTCCTC AGCTCCTGCT GGAGCACTAC 
ATCCTGGAGG GCCGTGGTGC CCGTTGCAAC GTAGTGATCA CCCAGCCGAG GAGGATCAGC 
GCCATCTCGG TTGCCCAGCG CGTGGCACAA GAATTGGGTC CCAACATGAG GAAGAATGTG 
GGCTACCAGG TGCGACTGGA GAGCAAGCCG CCCGCCAGGG GAGGTGCTCT GCTCTTCTGC 
ACAGTGGGCA TCCTGCTGCG GAAGCTGCAG GGGAATCCCA GCCTGGAGGG TGTCAGCCAC 
GTTGACAGGT ATTCCCTGCT AACCCTGCTG AAAGGCATCC AGAAGCTCAA CCCTGACCTG 
CGCCTGGTTT TGATGAGCGC CACAGGAGAC AACCAGCGCT TCTCGCACTA CTTTGGGGAT 
TGCCCTGTGG TCAAGGTGCC GGGCTTCATG TATCCAGTGA AGGAGTACTA CCTGGAGGAG 
ATCTTGGCCA AGCTGGGCCG GCACCGACAC CGGCACTATG AGATCAAGCA ATCGGATGAT 
GAATGTGTCC TTGACCTTGA TCTGATCACT GACCTTGTAC TCCAGATTGA CGCCCATGGA 
GAACCAGGCG GGATCCTCTG CTTTCTTCCT GGTTGGCAGG AAATCAAAGG GGTGCAGCAG 
CGCCTGCTAG AGATGCTTGG ATCTCAGAAC AGCCGTTACC TTGTCTTACC AGTGCACTCC 
AACATCCCCA TGATGGACCA GCAAAACATT TTCCAGCGGC CTCCGCCTGG TGTCAGAAAG 
ATTGTCCTGG CCACCAACAT CGCTGAGACC TCCATCACCA TCAACGACAT TGTCCACGTG 
GTGGACAGTG GCACGCATAA GGAGGAACGC TATGACCTAA AGACCAAGGT GTCCTGCCTG 
GAAACAGTGT GGGTGTCCAA GTCAAATGTC GTACAGCGGC GAGGGCGTGC TGGCCGCTGT 
CAGTCGGGAT TTGCCTACCA CCTCTTCCCT CGCAGCCGCC TGGACAAGAT GCCAACTTAC 
CAGGTTCCAG AGATCCTTCG CACCCCACTG GAGAATCTGG TGGTTCAGGC CAAGATCCAC 
ATGCCAGAGA AAACAGCAGT TGAATTTCTC TCTAAGGCTC TGGACAGCCC TGACATTAAA 
GCTGTGGATG AAGCAGTGAT CTTGCTGCAG GAGATTGGAG TGCTGGACCA GCGAGAAGCC 
CTCACCACTC TAGGCAAACG CCTTGCCCAG ATCTCCACAG ACCCTCGGCT GGCAAAGGCC 
ATCGTCTTGG CGTCCATCTA TCGCTGCCTC CACCCTCTGC TTGTCATCGT TTCCTGCCTC 
ACTCGGGACC CCTTCAGCAG CAGCCTGCAA AACCGTGCAG AGGTGGACAA GGCCAAGGCT 
GTTCTAAGCC GGGAGAGCGG GAGTGATCAC CTGGCGTTTG TCAGAGCTGT GGCAGGCTGG 
GAGGAGGTGC TCAGGCGCAG AGACAGTCGC GCCAGAGATA ACTACCTGCA GGACTATTAT 
CTCTATGGCC CCAGCCTTCG CTTCATCAAT GGCCTTGTCA AGCAGTTCTC TGAGAACCTC 
TATGAAGCCT TCCTGGTGTC ATCCCCATCT GACTGTACCA TGCCCTCGTC TGTGTGTAAC 
CAGTACAGTG AAGAGGAAGA ACTGGTCAAA GGAGTCCTTA TGGCTGGGCT CTATCCCAAC 
CTCATCCAGG TGAGACAAGG CAAGGTGACC CGTCAGGGGA AGTTCAAACC CAACAGCTAC 
GCGTATCGGA CAAAGGCTGG CACCGTTCTG CTCCACAAGT CTACAATCAA CAGGGAGGCA 
TCCAAGCTGT ACAGTCGCTG GCTAACGTAC TTCATGGCGG TGAAGTCCAA CGGTGGGGTG 
TTTGTACGGG ACTCCTCCCA GGTCCACCCG CTGGCTGTAC TGCTCATGAC TGACACAGAT 
ATCCATGTCC GAGATGATGG CTGGCGAGCA ACAGTCTCCC TGACAGACAG TGATCTCCTG 
GTGCTGGAGG GAGACTCCTA CACCATCCGC CTGCTGCGTG ATTTCCGCGT GTCACTCTCC 
AAGATGGTGG AGACGTGCCT GTGCTATGAG ATGGCAGCCA TCCCTGGGGA CCTGCACCAC 
CAGCACAGCC AGCTGCTTGA CATCCTGGTG GATCTGCTCA AAGGCCCTCC TGGCAGCTTT 
GGTGCTTAG