plcb2 cDNA ORF clone, Seriola lalandi dorsalis

Gene
Clones
gRNAs

Summary

Gene Symbol	plcb2
Entrez Gene ID	111652449
Full Name	phospholipase C beta 2
Gene Type	protein-coding
Organism	Seriola lalandi dorsalis

mRNA and Protein(s)

mRNA	Protein	Name
XM_023402714.1	XP_023258482.1	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2

Pathways from BioSystems
Gene Ontology

The following plcb2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plcb2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe182156	XM_023402714.1 Latest version!	Seriola lalandi dorsalis phospholipase C beta 2 (plcb2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

plcb2 ( XM_023402714.1 ) cDNA ORF clone, Seriola lalandi dorsalis -> XP_023258482.1 Seriola lalandi dorsalis phospholipase C beta 2 (plcb2), mRNA.

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CloneID	OSe182156
Clone ID Related Accession (Same CDS sequence)	XM_023402714.1
Accession Version	XM_023402714.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-25
Organism	Seriola lalandi dorsalis
Product	1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019524535.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Seriola lalandi dorsalis Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAAGA AAAGATACTT TCTGGAACCT CCAGAGGTCA AAGACTACCT GGTCAAAGGC 
GACAGATTTA CCAAGTGGTC AGAGGACTCC ACAAAGACCG TTCCTGTCAC CATGAAGATG 
GATCCGAAAG GTTTTTATGT CTACTGGATC AACCAAAGCA AGGAAACAAC GTTCCTGGAT 
GTTGCCACCA TCAGAGATAC CCGAATAGGA AAACATGCAA AACTTCCCAA ACACCCCAAA 
GTTCGCAACG TGTTCAACCT GGACTTTCCA GACAGCAACC ATCTCTCCAA AACCCTGACC 
ATAGTCTCAG GCATGGACAC AGTGAATCTG ACTTACTACA ACTTCTTTGC CTCAAAGGAG 
AAAGTGACAC AGAACTGGGC AAATGACATT CTTGCAATTG CCTACAACGC TGCAAGAAAC 
AATGCATGCA GACAAGTCTT CCTGGATAAA ATGTACGTCC GCATCTCTCT TCACACCAAC 
AAGGACGGCA AGATCCCGGT GAAAAATATT TACAAGATGT TCCCTGCGGA TAAGAAGAGG 
GTGGAAAGTG CCTTAGCATC AGCGCACCTC CCTAAAGGAA AGCATGACAC CATGAAGCCT 
GATGTGTTCA CTGAGGCTGC CTTCAAGGCC TTTCTGACGC ATCTCTGCCC ACGGCCAGAG 
ATCTACGAGA TCTTCACTTC TTACTCCACA AAACCCACCA TGACAAAGGA GAATTTCACC 
AAGTTTCTCA ACGAGAAACA GAGGGACTCC CGGCTCAACG AGGAGCTTTT TCCACGTCTG 
CGGCAGGACC AGATCAAGGC TCTGATCGAC AAATATGAAC CCTGTTCTAA CAATACCAAC 
AGAGGCCTGA TCTCTCCAGA AGGTCTTCTG TTCTACCTGA TGGGGTCGGA GACATCGGTT 
GTGGTGCAGG ACAGGCTGGC TAAGTGTCAG GAAATGACCC AACCCATACC CCACTACTTC 
ATCAAGTCCT CCCACAACAC ATACCTGACA GCTGGTCAGT TCTCCGGTGT GTCCTCTCCG 
GAGATGTACC GTCAGTGTCT GCTGTCCGGC TGCCGGTGTC TGGAGCTGGA CTGCTGGAAG 
GGCAAACCTC CAGATGAAGA GCCCATCATT ACCCACGGCT TCACCATGAC AACGGAGATC 
CTCTTCAAGG ATGTGATTGA GGCCATAGCT GAGAGCGCCT TCAAGACCTC ACAGTACCCT 
GTTATCCTCT CGTTCGAGAA CCACGTCGAC TCGGTGAAAC AGCAGGAGAA GATGGCCAAC 
TACTGCAAAA CCATATTTGG TGATGCCCTG CTAACAGAGC CGCTGGACAA ATACCCTCTG 
AAGCCAGGCC AGCAGATCCC CAGCCCATCT GAGCTCATGG GCAAGATCCT CATCAAGAAC 
AAGAAGGGCA GCCACGAAAA ACCAGCCCAG ACTAAGAAAA CCACCACAGC AGCCACCACC 
GACCAGACAA CAACCACAAC CACAACCACA GCTGCACCCA ACCAGGTCCC AAACAGCACT 
CCTCAGGATC CAGCAAACCC AGCACCCAGC ACCCAGGAGA CCCAAGAGGG CAACGCAGCT 
GTGGAGGAGA ACGAGGAGCA AGAAGACACA GAGGAACAGG ATGAGGAGAA AATGAAGACA 
TCAGATGAGG GCACAGCTGG ACAAGAAGTG ACAGCGTACG AGGCAATGTC ATCCCTGGTC 
AACTACATCC AGCCCAGCAA ATTCATCTCT TTTGACAATG CCAGAAAGAA AAACAAGAGT 
TACGTCATCT CGTCTTTTGT GGAGACCAAA GGGGAGGCAA TGATTTCCAA GACTGCTGTT 
GAATTTGTGG AATACAATAA GAGGCAGATG AGCAGGATTT ACCCCAAAGG AACAAGGATG 
GACTCATCCA ATTACAGCCC ACAGCCTTTC TGGAATGCAG GCTGCCAGAT GGTGGCGCTC 
AACTACCAGA CCATGGATTT CCCCATGCAG CTGAACATGG CTCTGTTTGA GTTCAACGGC 
AGAACGGGTT ACCTCCTCAA GCACGATGTC CTGCGCCGCA GCGACAAGAA GTTTGACCCT 
TTCTGTGACA GGATTGACAC GGTTGTGGCA AGCACGCTGA CCATAAAGAT CTATTCGGGC 
CAGTTTCTGT CTGACAAGAG TGTGAAAACA GGGGTGGAGG TGGAGGTGAT CGGGCTGCCG 
GGAGACCCCA AAAAGAAATA TCGCACCAAG TGGTCCAGCA CGCCCAACGC CATTAACCCA 
GTGTGGAATG AGGAGCCTTT TGTTTTTGAG AAGATCCTGC TCCCAGAAAT GGCCTCTCTA 
AGAATCGTAG TCCATGAGGA GAACGGTAAA TTCTTGGCAC ACAGGATCAT CCCACTTGAT 
GCCATCCAAT CAGGCTTCCA TCACATCTGC CTGCGCAGTG AGAGCAACTT GCCGCTCACT 
TTGCCTGCTC TCTTTGTCTA CATCGAGGTC AAGGACTACA TCCCTGCTGC CTTTGCAGAT 
TTCACAGATG CCTTATTTAA CCCAACAAAG GGCACAGAGA AGACAACAAA GACCCCAAAG 
GAGTCATCAG CTGACTACAT TTCCCCCTAC GAGTTGCCTC TTGCGGCCGA AACCCCCACG 
GACAAAGCTA AGGAAAGTGA AGCCCCCGTC ATAGAAAAGG CTGCATCTGA ACCGACACCG 
CCTGTTGATG CCACTGACCA ATCGTCCCAG TCGGCTCCAG AAACAAGTGC AGAAGAAGCT 
AAAGAAGAGG CAGTGAACAC AACAGATACT CCACAGCCTG CAGCAGAGCC TCTTCAAACT 
CCTGATACAG CTGCTGCTGC TGCTGCTGCT GCTGAAGATC CAGCACCAGA TGCAGGAACA 
TCTGACCCCC TTCCCGAAGT CACTCCTTCC CCTGAGGAGG AGGCTGCCCC TGAAAAAGAA 
GCGCCCCCGG AGGCTGCCTC TGAAAAAGAA GCGCCCTCAG AGTCAGAGGC AGCTCCCGAA 
ACCAAAGATG AACCGGTAAA TGACTCCAGC ACTGACCCTG AGCCTGCTTC AGATGATAAA 
GCAGGCACAG AGGAGGCTTC AGGAGCGCCT GCTGCTGCTG CTGCTGTGGT CGTGCCCTGT 
CCGGCGAGCA CATCGCCAGA GTCTGCAGGA TCGAATGACT CATCTGAGCC GCCGGCGTGC 
AGTGAAGAGC CTTCGACTGT GACTACTGAG GAACTGACAC AGCACAAGAA CTATCTGAAG 
GTCATCAAGC GCCAGGAGAA AGAGATGAAA GAAACCGAAA AGAAGTATCA GAAAAAAGGA 
GAGGACCTGA TTCAAAAATA CTCTGACTCC TTCAAGGCCA TGATGAAGAA AACCTCTCTA 
AAGAAAAAAG AGGGAGGGGG AGGCACCTCG GACTCCAGCG TGAAGACGGA GCAGGTGAAG 
GAGCAGAAAG AGAAAATGCA GGTCGAGCTG CAAGCTCTGT GGACGGAGCA GAACGATCAG 
CTGAAGAAGA AGAAAGAGCA GTTTGCTACA GAGAGACTGA CCAAACTGTT GGAGATGGCC 
ACAGAGAGAC ACACCAGTGA AGTGAAGACC CTGGAGAGTG AAGCCAAAGA TAAAATGAAG 
AAAACGCTCT CAAAATCTTC ATCCTCAAAG AAAGCAAAGC TTAAAAAGGC CATGAGCACC 
GAGATGTTGG ATGAGAACAG TCAGTCAGAT GGTGCATCCT CAGATCACAG CCCGCAGCAA 
GAGGCTCTGA AGAAGAAACA GGCAGCTACA CTGGAAGAAA TCAAGACCCT GACTAATCAG 
CTCAATCAGG AGGCCCTGAA GGAGCACGAG CAGAAACTGA GGTCTCTGCC AGCGGAGGTG 
AGGGATGCAG TTAATGTCTG TGTGGGGGCT CACTTTCCTG AACTGGTTGA CCAGGCTGGA 
GACAAGAAGG TGGAGGGAGG CGGCTTCTAT GGAGATGTCT TCCTTGGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023258482.1
CDS	47..3937
Translation	MNKKRYFLEPPEVKDYLVKGDRFTKWSEDSTKTVPVTMKMDPKGFYVYWINQSKETTFLDVATIRDTRIGKHAKLPKHPKVRNVFNLDFPDSNHLSKTLTIVSGMDTVNLTYYNFFASKEKVTQNWANDILAIAYNAARNNACRQVFLDKMYVRISLHTNKDGKIPVKNIYKMFPADKKRVESALASAHLPKGKHDTMKPDVFTEAAFKAFLTHLCPRPEIYEIFTSYSTKPTMTKENFTKFLNEKQRDSRLNEELFPRLRQDQIKALIDKYEPCSNNTNRGLISPEGLLFYLMGSETSVVVQDRLAKCQEMTQPIPHYFIKSSHNTYLTAGQFSGVSSPEMYRQCLLSGCRCLELDCWKGKPPDEEPIITHGFTMTTEILFKDVIEAIAESAFKTSQYPVILSFENHVDSVKQQEKMANYCKTIFGDALLTEPLDKYPLKPGQQIPSPSELMGKILIKNKKGSHEKPAQTKKTTTAATTDQTTTTTTTTAAPNQVPNSTPQDPANPAPSTQETQEGNAAVEENEEQEDTEEQDEEKMKTSDEGTAGQEVTAYEAMSSLVNYIQPSKFISFDNARKKNKSYVISSFVETKGEAMISKTAVEFVEYNKRQMSRIYPKGTRMDSSNYSPQPFWNAGCQMVALNYQTMDFPMQLNMALFEFNGRTGYLLKHDVLRRSDKKFDPFCDRIDTVVASTLTIKIYSGQFLSDKSVKTGVEVEVIGLPGDPKKKYRTKWSSTPNAINPVWNEEPFVFEKILLPEMASLRIVVHEENGKFLAHRIIPLDAIQSGFHHICLRSESNLPLTLPALFVYIEVKDYIPAAFADFTDALFNPTKGTEKTTKTPKESSADYISPYELPLAAETPTDKAKESEAPVIEKAASEPTPPVDATDQSSQSAPETSAEEAKEEAVNTTDTPQPAAEPLQTPDTAAAAAAAAEDPAPDAGTSDPLPEVTPSPEEEAAPEKEAPPEAASEKEAPSESEAAPETKDEPVNDSSTDPEPASDDKAGTEEASGAPAAAAAVVVPCPASTSPESAGSNDSSEPPACSEEPSTVTTEELTQHKNYLKVIKRQEKEMKETEKKYQKKGEDLIQKYSDSFKAMMKKTSLKKKEGGGGTSDSSVKTEQVKEQKEKMQVELQALWTEQNDQLKKKKEQFATERLTKLLEMATERHTSEVKTLESEAKDKMKKTLSKSSSSKKAKLKKAMSTEMLDENSQSDGASSDHSPQQEALKKKQAATLEEIKTLTNQLNQEALKEHEQKLRSLPAEVRDAVNVCVGAHFPELVDQAGDKKVEGGGFYGDVFLG

Target ORF information:

RefSeq Version	XM_023402714.1
Organism	Seriola lalandi dorsalis
Definition	Seriola lalandi dorsalis phospholipase C beta 2 (plcb2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023402714.1

ORF Insert Sequence:

ATGAATAAGA AAAGATACTT TCTGGAACCT CCAGAGGTCA AAGACTACCT GGTCAAAGGC 
GACAGATTTA CCAAGTGGTC AGAGGACTCC ACAAAGACCG TTCCTGTCAC CATGAAGATG 
GATCCGAAAG GTTTTTATGT CTACTGGATC AACCAAAGCA AGGAAACAAC GTTCCTGGAT 
GTTGCCACCA TCAGAGATAC CCGAATAGGA AAACATGCAA AACTTCCCAA ACACCCCAAA 
GTTCGCAACG TGTTCAACCT GGACTTTCCA GACAGCAACC ATCTCTCCAA AACCCTGACC 
ATAGTCTCAG GCATGGACAC AGTGAATCTG ACTTACTACA ACTTCTTTGC CTCAAAGGAG 
AAAGTGACAC AGAACTGGGC AAATGACATT CTTGCAATTG CCTACAACGC TGCAAGAAAC 
AATGCATGCA GACAAGTCTT CCTGGATAAA ATGTACGTCC GCATCTCTCT TCACACCAAC 
AAGGACGGCA AGATCCCGGT GAAAAATATT TACAAGATGT TCCCTGCGGA TAAGAAGAGG 
GTGGAAAGTG CCTTAGCATC AGCGCACCTC CCTAAAGGAA AGCATGACAC CATGAAGCCT 
GATGTGTTCA CTGAGGCTGC CTTCAAGGCC TTTCTGACGC ATCTCTGCCC ACGGCCAGAG 
ATCTACGAGA TCTTCACTTC TTACTCCACA AAACCCACCA TGACAAAGGA GAATTTCACC 
AAGTTTCTCA ACGAGAAACA GAGGGACTCC CGGCTCAACG AGGAGCTTTT TCCACGTCTG 
CGGCAGGACC AGATCAAGGC TCTGATCGAC AAATATGAAC CCTGTTCTAA CAATACCAAC 
AGAGGCCTGA TCTCTCCAGA AGGTCTTCTG TTCTACCTGA TGGGGTCGGA GACATCGGTT 
GTGGTGCAGG ACAGGCTGGC TAAGTGTCAG GAAATGACCC AACCCATACC CCACTACTTC 
ATCAAGTCCT CCCACAACAC ATACCTGACA GCTGGTCAGT TCTCCGGTGT GTCCTCTCCG 
GAGATGTACC GTCAGTGTCT GCTGTCCGGC TGCCGGTGTC TGGAGCTGGA CTGCTGGAAG 
GGCAAACCTC CAGATGAAGA GCCCATCATT ACCCACGGCT TCACCATGAC AACGGAGATC 
CTCTTCAAGG ATGTGATTGA GGCCATAGCT GAGAGCGCCT TCAAGACCTC ACAGTACCCT 
GTTATCCTCT CGTTCGAGAA CCACGTCGAC TCGGTGAAAC AGCAGGAGAA GATGGCCAAC 
TACTGCAAAA CCATATTTGG TGATGCCCTG CTAACAGAGC CGCTGGACAA ATACCCTCTG 
AAGCCAGGCC AGCAGATCCC CAGCCCATCT GAGCTCATGG GCAAGATCCT CATCAAGAAC 
AAGAAGGGCA GCCACGAAAA ACCAGCCCAG ACTAAGAAAA CCACCACAGC AGCCACCACC 
GACCAGACAA CAACCACAAC CACAACCACA GCTGCACCCA ACCAGGTCCC AAACAGCACT 
CCTCAGGATC CAGCAAACCC AGCACCCAGC ACCCAGGAGA CCCAAGAGGG CAACGCAGCT 
GTGGAGGAGA ACGAGGAGCA AGAAGACACA GAGGAACAGG ATGAGGAGAA AATGAAGACA 
TCAGATGAGG GCACAGCTGG ACAAGAAGTG ACAGCGTACG AGGCAATGTC ATCCCTGGTC 
AACTACATCC AGCCCAGCAA ATTCATCTCT TTTGACAATG CCAGAAAGAA AAACAAGAGT 
TACGTCATCT CGTCTTTTGT GGAGACCAAA GGGGAGGCAA TGATTTCCAA GACTGCTGTT 
GAATTTGTGG AATACAATAA GAGGCAGATG AGCAGGATTT ACCCCAAAGG AACAAGGATG 
GACTCATCCA ATTACAGCCC ACAGCCTTTC TGGAATGCAG GCTGCCAGAT GGTGGCGCTC 
AACTACCAGA CCATGGATTT CCCCATGCAG CTGAACATGG CTCTGTTTGA GTTCAACGGC 
AGAACGGGTT ACCTCCTCAA GCACGATGTC CTGCGCCGCA GCGACAAGAA GTTTGACCCT 
TTCTGTGACA GGATTGACAC GGTTGTGGCA AGCACGCTGA CCATAAAGAT CTATTCGGGC 
CAGTTTCTGT CTGACAAGAG TGTGAAAACA GGGGTGGAGG TGGAGGTGAT CGGGCTGCCG 
GGAGACCCCA AAAAGAAATA TCGCACCAAG TGGTCCAGCA CGCCCAACGC CATTAACCCA 
GTGTGGAATG AGGAGCCTTT TGTTTTTGAG AAGATCCTGC TCCCAGAAAT GGCCTCTCTA 
AGAATCGTAG TCCATGAGGA GAACGGTAAA TTCTTGGCAC ACAGGATCAT CCCACTTGAT 
GCCATCCAAT CAGGCTTCCA TCACATCTGC CTGCGCAGTG AGAGCAACTT GCCGCTCACT 
TTGCCTGCTC TCTTTGTCTA CATCGAGGTC AAGGACTACA TCCCTGCTGC CTTTGCAGAT 
TTCACAGATG CCTTATTTAA CCCAACAAAG GGCACAGAGA AGACAACAAA GACCCCAAAG 
GAGTCATCAG CTGACTACAT TTCCCCCTAC GAGTTGCCTC TTGCGGCCGA AACCCCCACG 
GACAAAGCTA AGGAAAGTGA AGCCCCCGTC ATAGAAAAGG CTGCATCTGA ACCGACACCG 
CCTGTTGATG CCACTGACCA ATCGTCCCAG TCGGCTCCAG AAACAAGTGC AGAAGAAGCT 
AAAGAAGAGG CAGTGAACAC AACAGATACT CCACAGCCTG CAGCAGAGCC TCTTCAAACT 
CCTGATACAG CTGCTGCTGC TGCTGCTGCT GCTGAAGATC CAGCACCAGA TGCAGGAACA 
TCTGACCCCC TTCCCGAAGT CACTCCTTCC CCTGAGGAGG AGGCTGCCCC TGAAAAAGAA 
GCGCCCCCGG AGGCTGCCTC TGAAAAAGAA GCGCCCTCAG AGTCAGAGGC AGCTCCCGAA 
ACCAAAGATG AACCGGTAAA TGACTCCAGC ACTGACCCTG AGCCTGCTTC AGATGATAAA 
GCAGGCACAG AGGAGGCTTC AGGAGCGCCT GCTGCTGCTG CTGCTGTGGT CGTGCCCTGT 
CCGGCGAGCA CATCGCCAGA GTCTGCAGGA TCGAATGACT CATCTGAGCC GCCGGCGTGC 
AGTGAAGAGC CTTCGACTGT GACTACTGAG GAACTGACAC AGCACAAGAA CTATCTGAAG 
GTCATCAAGC GCCAGGAGAA AGAGATGAAA GAAACCGAAA AGAAGTATCA GAAAAAAGGA 
GAGGACCTGA TTCAAAAATA CTCTGACTCC TTCAAGGCCA TGATGAAGAA AACCTCTCTA 
AAGAAAAAAG AGGGAGGGGG AGGCACCTCG GACTCCAGCG TGAAGACGGA GCAGGTGAAG 
GAGCAGAAAG AGAAAATGCA GGTCGAGCTG CAAGCTCTGT GGACGGAGCA GAACGATCAG 
CTGAAGAAGA AGAAAGAGCA GTTTGCTACA GAGAGACTGA CCAAACTGTT GGAGATGGCC 
ACAGAGAGAC ACACCAGTGA AGTGAAGACC CTGGAGAGTG AAGCCAAAGA TAAAATGAAG 
AAAACGCTCT CAAAATCTTC ATCCTCAAAG AAAGCAAAGC TTAAAAAGGC CATGAGCACC 
GAGATGTTGG ATGAGAACAG TCAGTCAGAT GGTGCATCCT CAGATCACAG CCCGCAGCAA 
GAGGCTCTGA AGAAGAAACA GGCAGCTACA CTGGAAGAAA TCAAGACCCT GACTAATCAG 
CTCAATCAGG AGGCCCTGAA GGAGCACGAG CAGAAACTGA GGTCTCTGCC AGCGGAGGTG 
AGGGATGCAG TTAATGTCTG TGTGGGGGCT CACTTTCCTG AACTGGTTGA CCAGGCTGGA 
GACAAGAAGG TGGAGGGAGG CGGCTTCTAT GGAGATGTCT TCCTTGGTTA A