sec71 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	sec71
Entrez Gene ID	2543610
Full Name	Sec7 domain protein, ARF GEF (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001020385.2	NP_594954.1	putative Sec7 domain-containing protein sec71

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ARFGEF2	XP_534448.1
Arabidopsis thaliana (thale cress)	AT4G38200	NP_195533.2
Pan troglodytes (chimpanzee)	ARFGEF2	XP_001165517.2
	ARFGEF2	XP_002798173.1
Schizosaccharomyces pombe (fission yeast)	sec71	NP_594954.1
Arabidopsis thaliana (thale cress)	AT4G35380	NP_195264.2
Homo sapiens (human)	ARFGEF2	NP_006411.2
Rattus norvegicus (Norway rat)	Arfgef2	NP_851597.2
Danio rerio (zebrafish)	arfgef2	XP_002666513.2
Gallus gallus (chicken)	ARFGEF2	XP_417388.3
Xenopus tropicalis (tropical clawed frog)	arfgef2	XP_002933226.2
Bos taurus (cattle)	ARFGEF2	NP_001192581.1
Mus musculus (house mouse)	Arfgef2	NP_001078964.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sec71 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sec71 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc103094	NM_001020385.2 Latest version!	Schizosaccharomyces pombe putative Sec7 domain-containing protein sec71 (sec71), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sec71 ( NM_001020385.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594954.1 Schizosaccharomyces pombe putative Sec7 domain-containing protein sec71 (sec71), mRNA.

Price & Availability↑

CloneID	OSc103094
Clone ID Related Accession (Same CDS sequence)	NM_001020385.2
Accession Version	NM_001020385.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative Sec7 domain-containing protein sec71
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001020385.1.

ATGACAGATT TGGAGATAGA AACCGTTAGT AGGGTTTCTA GTAATCCTGA TGAAGTAAAA 
GGATCGTCTG TTGTCGAAGA AGAACCGGAT AGTGAATCAA CAATCAAAAG TCGAGTTTCT 
GATGAAATAG ATGAACATGA TTCCATACCT GAACAGTCTA ATACTGAAAA AAGTATAGAA 
ATTAACGATA AAAACTTAGA AGCGGAAAAA GATATTGAAA GCAATCTATC TCTAAGACCT 
CCAGAAGATG ACTTAGATTC ACGTTCGATT GAAAGTGAAC AAACAGGTAC TCTGAGCAAA 
CAGACTACAT CTACTTCTGA AGCTCCACAA GATCTAAAGA TTTGGAAGAA TATTTCAAAC 
GCCTCTAACC AAAACTCTGG CAAACTCAAT CCTCAACTCT TCGTAATTGA GGCTTTTAAG 
CATATGTCAA AAGCAAAGGC TACAAAACGG CATAAGAAGC TTAGGGAGGC TATCAATAAT 
GTCCAAATAG AATTACAAAA GCAGCCATTT CTTCTTCCCG AAGTCATTTT GGAACCTTTG 
GTAATGGCTT GTCAAACAAA TAGTACAACG TTGCTGACTA TTACTTTGGA TTGCTTTGCC 
AAGCTGATTG ATTACAATTA TTTTGATTCC CCAACATTAA ATCCATCGGA TATTACCTTA 
ATGGAACGTG TGGTTAACAC TATTGCATCC TGTTTTTGCG GTGAGTCTAC TCCAGAAAGA 
GTGCAGCTAC AAATTGTAAA GGCTTTATTG GCAGCTATTA CTTCTGAAAG GACAATCATT 
AGACATTCGT TTTTACTTAC AGCAGTGAGG CAAACTTACA ATATTTTCCT CTTATGTAAA 
GACTCAACTA CCCAAGCCAT TGCTCAGGTA GCTCTTTTAC AAATGGTTGA TTCAGTATTC 
CAGCGCCTTT CTACCGTTCT TAATCATGAA AGAGAATTTT CTACTATTAA CATGAATAAA 
TCATCCAGTA ATGGAACTCC TGATCGTGCT AACTCTCCTA TACCTTCTCA ATTATCAGAA 
AACAAACTCA CACTTGAATC TTTCGAGCAC AGAAAAAGCT TTGACCAGGT TCGTGAAGAA 
GCTCCTTTGG AAGAGGATTC TTTGGAGCAA CAGTTGCTTC GGGATGCATT TTTGTTAATC 
AGAGCTTTAT GTAAGCTTTC TATTAAAAAC ATTCCGTATG AGCATGAATA CGACCTAAAG 
TCGCAATCGA TGCGATCAAA GTTGATGAGC TTGCATCTTA TATATCATAT TCTTCGAACT 
TATATGAACA TCCTTTCGGA CATAAATGTG AAAATAAGGT CTCCAACTTC AACTCCGACA 
CCATTGATTG ACGCTGTTAA ACAATACATT TGCCTTGCAT TGGCTAAAAA TGTGGTTAGC 
CATGTTTTAC CGGTATTCGA GATTTCTTGT GAAATATTTT GGTTAATTCT TTCTGAATTA 
AAAAATTTTT TCAAGTCAGA GTTAGAAGTG TTTTTTACCG AAATCTTCTT TCCCATATTG 
GAAATGCGAA CCTCTAGCAA TCAACAAAAA ATTGTACTGC TGAATATTTT TCACCGCATG 
TGTGAAGAAC CACAGACCTT AATTGAACTT TATCTTAATT ATGACTGTAT AAGTGGTAAT 
ACTGAAAATA TTTATGAAAG AGCAATTGTT ACTCTATCAA GGATTGCAAG TCAGAGTACT 
TCTGATCCTC CTCCTTCATT TGTTTTCCGT GATGACCAGC TAGTAATTGA TAAACCGGGG 
TTTGTATATC ATACATTGAA TGATATTCCT CAGCTTAATT CGTCAACAAT TGGAAGCTAT 
GTTCATTCGC ACAATCCACC TTACTTTGAT TATCAAATTC GGTTGAAGTC TTATAGATGT 
TTAATATCTA CTTTATCTTC TCTATTTACA TGGTGTAATC AAACATTTGC GCCTACTGTC 
GAAATAACGG CGAAGGACGA CGAAACAGAG TCGACTTCAA AAGGAGAAGA ACCACAGAAA 
AGTAAATCAG AGCCCCCTTC CGCAGGTATA AATAGTACAT CAATGGATAA TTTGGAATCT 
TCTGGTCAGG CTTTAGCTAC AGACGATCCT TCTCAGTTTG AAAATTTAAA ACATCGAAAG 
AAACAATTAC AAGAAGCAAT ACAAAAATTT AACTACAAAC CAAAAGAGGG AATTAAGATT 
CTACTTTCAA GTCATTTTAT TGCTTCCAAA ACTCCTACCG ATATTGCTAA ATTTTTAATC 
TCTACAGAAG GCTTGGATAA AGCTGTGCTA GGCGAGTATT TAGGAGAAGG AAATGACGAG 
AATATTGCTA TCATGCACTC TTTCGTTGAC CACATGAGTT TCAATGATAT TCCATTTGTG 
AATGCCCTTC GTTCATTTTT ACAAAAATTT CGTTTACCCG GTGAAGCGCA AAAGATTGAC 
AGATTCATGC TCAAGTTTGC AGAAAAATAT ATTGATGACA ATCTTGGTGT TTTTAAAAAT 
GCAGACACTG CTTATATTCT AGCATATTCA ATTATTATGT TAAATACTGA TTTACATTCT 
CCCCAAGTTA AAAATCGGAT GACATGTCAA GATTTTATTA AAAATAACAG AGGTGTGGAT 
GATGGTGCTA ATCTGAGCGA TTCGTTTTTG ACTGAGGTAT ATGAGGAAAT CCAAAAAAAC 
GAAATTGTTT TGAAAGATGA GCAAGATCCT ACTTCCAACT TTCCAGAAAT ACCCGGGACT 
AGTAATCTTA GCTTTGCTGC TAACATAAGT AATGCATTGG CGACGGTTGG AAGAGATTTG 
CAAAGGGAAG CATACTATAT GGCTTCCAAT AAGATGGCCA ACAAGACCGA AGCGTTGTTT 
AAAGATTTAA TTCGTGAACA ACGTGAAAGA GGAAAACTTT CAGGAAATGA TATTTATTAT 
ACTGCTAGGC ACTTTGAGCA TGTTTGTCCG ATGTTTGAAG CTGTTTGGAT GCCAATTCTC 
GCGGCATTCT CTGAACCATT GCAACTCTCC TCTGATCCAG CATTGATTCA ACTTTCACTT 
GATGGATTTC GGTTGGCCAT GAACGTCATT TTCTTTTTCT CAATGGATTT ACCTAGAAAT 
GCTTTCATGC AAACTTTGAC TAAGTTTACT CATTTGAATA ATACGTCCGA GTTAAAATGG 
ACGAATATGC ACGCTTTGAA AACCCTACTT GAAATTTCAC TAGCTCATGG AGATAAGTTG 
AGAGACTCTT GGAAAGACGT GCTTTTATGT ATAAGTCAGT TGGAACGTGT CCAACTAATA 
TCTGCTGGTG TCGACATAAA TTCTTTACCA GATGTAAGTA CTACCAAACC ATTGCGCAAA 
TCTTTAGATA AGAATATACG ACAGTCTCGA AGTGGAAGCA TTTCGTTAAA ACATTCTAAA 
AGTTTTCAAT CTGCCTCCAC TCACTCCACT AAATCTAGCT CAGTTGAAAT CGTCAGAGAA 
TATAGTTCTC GTGAAGTTGT AATGGCTGTT GATATGCTCT TCTCAAACAC TCGAAACCTT 
GGTAGTGAAG GAATCTACGA TTTTGTAAAA GCATTAATTG AGGTCTCTTG GGAAGAAATA 
GAGTGTTCTT TGGAACTTTC TAATCCGAGG TTATTCAGTT TACAAAAGTT GGTTGAAATA 
TCATATTATA ATATGCGTCG TATCCGTATG GAATGGTCCA GTATTTGGTC TTTGTTAGGT 
ACTTATTTTA CCCAAGTCAG CTGTCATGAA AACTCAATAA TAGCATCTTT TGCTTTAGAT 
TCCTTGAGAC AATTTTCTAT GCAGTTTTTA GAAATTGAAG AGCTCTCTCA CTTTAAATTT 
CAGAAGGACT TCTTACAGCC ATTTTCACAT GCTATGGAAA ATTCGCAGGA CCTTAAGATT 
AAAGACCTTG TCTTACGTTG TATTGATCAA ATGATCAAAG CCAGATACCA AAACATACGT 
TCTGGATGGA GAACCATATT CCATATTCTC GCCTACGCTT CTAAAATTGA AAATCTTTTG 
GTTTTGCAGT GCGCAATATC TGTTGTATCA TCTTTGGGCC ATGAACATAT CAGCTGCGTC 
CTTACACAAG GGGCTTATAT TGATCTGATA TCTTGTATAA CTAAGTTTGC TAAACTTAAT 
GGCAATCAAA AGTTCTGTCT GAGTTGTGTG GATATGCTTA AAAATTTGGA GCATGAGTTA 
ATTAAGCATT TGAAGCACAT GAAAAAAGAG AGTGTATATT CAAAAAAACT AGAAGAAGAG 
TACTGGCTTC CGTTTTTGCT GTCATTTAAT GAAATCATCT GCGAGGCGTC AGATTTAGAG 
GTGCGATCTA AAGCCTTGAA GGTTTTATTC GATTGTCTTT ATCGACACGC TGATGACTTT 
GACGAAGAAT TTTGGGAAAC CGTTTCCAAT AAAGCTTTGC TCTCAATATT TTCTATTTTG 
TCCATTACTA ATTCCCAACG TTTGTATTTA GCAAAGAACA CTGAAGAGAC TGAAGTATGG 
ATGCTAACAA CAATGGTTGA AGCACTAAAA GCTTTTATTG AATTAATTAA AAACTTGTTT 
GAGCGCCTTC ATTTTTTGCT TCCTAAGGCG CTGAATTTAC TGGAAAAATG TATTTGCCAA 
GAAAACAGTA TGATATCAAA AGTGGGACTT AGTTGTTTTT CTCAATTTGT CCTCAAGAAT 
AAGAATCAAT TTAAAGACGT GGACTGGGAT GAAATAATTA ACTCGATAAA TCAGCTGCTT 
CAAATGACTC TTCCGATAGA ACTTCGTGAT CCTTCATTGT ATCCTCAAGT CAATTCTGAT 
AGTTCTCTTG AGGATGTCAA AGAAAATAGT TTTCGTCCAC ATGAAATATC AAGGTTTAAT 
AGCCAGAGCG TCTTTAAATC AAAAAAGCAC CATTTGAAAA GTATTGTTGT AAAGTGCACA 
TTACAACTTT TGATGCTTAA CTGCCTATGG GAATTATTTC ATTCTGACAA TATGCTTACC 
AATATTCCGA AAAGAAAAAT GGTAAAATTG CTGGATATCT TAAAGCAATC TTGGGAATTT 
GCTGAATCTT TTAATTCGGA TTTTGAAATA CGCGCTAAAA TCTTATCCTC GGGCATTGTG 
GAGCACATGC CCAATCTTTT AAGCCAAGAA GCCTTATGTG CTAAGCTATA TTTTTATACC 
GCATTTGAAT GTATGTCTTC TTTGAAGTCG GATTCCCATG ATACAGAAGA ATATAACGAT 
TTAATGGATG TTTTTCAGAA AAAAATTTAT CTTGCATCGC AATTGGTACT TCATGGTTTT 
CAAAGGGTAA TTGGTGATAA CCCTGTAAAG GGAGTTGCTG CATTCCAACC TGTAATAGCC 
GCCTTGGTTA GCTATATCAA TTCACTAGAC GAAATTCAAT TTTCTCGGGG AAAGTCCGAA 
TTCTATCAAT TACTGTGTGC CATTGTGGCT TGTGGACATA TTGACCAGCA ATTGGGAACT 
TCTCTAAGCA ACGCATTTTT AAGATATGCT TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594954.1
CDS	90..5525
Translation	MTDLEIETVSRVSSNPDEVKGSSVVEEEPDSESTIKSRVSDEIDEHDSIPEQSNTEKSIEINDKNLEAEKDIESNLSLRPPEDDLDSRSIESEQTGTLSKQTTSTSEAPQDLKIWKNISNASNQNSGKLNPQLFVIEAFKHMSKAKATKRHKKLREAINNVQIELQKQPFLLPEVILEPLVMACQTNSTTLLTITLDCFAKLIDYNYFDSPTLNPSDITLMERVVNTIASCFCGESTPERVQLQIVKALLAAITSERTIIRHSFLLTAVRQTYNIFLLCKDSTTQAIAQVALLQMVDSVFQRLSTVLNHEREFSTINMNKSSSNGTPDRANSPIPSQLSENKLTLESFEHRKSFDQVREEAPLEEDSLEQQLLRDAFLLIRALCKLSIKNIPYEHEYDLKSQSMRSKLMSLHLIYHILRTYMNILSDINVKIRSPTSTPTPLIDAVKQYICLALAKNVVSHVLPVFEISCEIFWLILSELKNFFKSELEVFFTEIFFPILEMRTSSNQQKIVLLNIFHRMCEEPQTLIELYLNYDCISGNTENIYERAIVTLSRIASQSTSDPPPSFVFRDDQLVIDKPGFVYHTLNDIPQLNSSTIGSYVHSHNPPYFDYQIRLKSYRCLISTLSSLFTWCNQTFAPTVEITAKDDETESTSKGEEPQKSKSEPPSAGINSTSMDNLESSGQALATDDPSQFENLKHRKKQLQEAIQKFNYKPKEGIKILLSSHFIASKTPTDIAKFLISTEGLDKAVLGEYLGEGNDENIAIMHSFVDHMSFNDIPFVNALRSFLQKFRLPGEAQKIDRFMLKFAEKYIDDNLGVFKNADTAYILAYSIIMLNTDLHSPQVKNRMTCQDFIKNNRGVDDGANLSDSFLTEVYEEIQKNEIVLKDEQDPTSNFPEIPGTSNLSFAANISNALATVGRDLQREAYYMASNKMANKTEALFKDLIREQRERGKLSGNDIYYTARHFEHVCPMFEAVWMPILAAFSEPLQLSSDPALIQLSLDGFRLAMNVIFFFSMDLPRNAFMQTLTKFTHLNNTSELKWTNMHALKTLLEISLAHGDKLRDSWKDVLLCISQLERVQLISAGVDINSLPDVSTTKPLRKSLDKNIRQSRSGSISLKHSKSFQSASTHSTKSSSVEIVREYSSREVVMAVDMLFSNTRNLGSEGIYDFVKALIEVSWEEIECSLELSNPRLFSLQKLVEISYYNMRRIRMEWSSIWSLLGTYFTQVSCHENSIIASFALDSLRQFSMQFLEIEELSHFKFQKDFLQPFSHAMENSQDLKIKDLVLRCIDQMIKARYQNIRSGWRTIFHILAYASKIENLLVLQCAISVVSSLGHEHISCVLTQGAYIDLISCITKFAKLNGNQKFCLSCVDMLKNLEHELIKHLKHMKKESVYSKKLEEEYWLPFLLSFNEIICEASDLEVRSKALKVLFDCLYRHADDFDEEFWETVSNKALLSIFSILSITNSQRLYLAKNTEETEVWMLTTMVEALKAFIELIKNLFERLHFLLPKALNLLEKCICQENSMISKVGLSCFSQFVLKNKNQFKDVDWDEIINSINQLLQMTLPIELRDPSLYPQVNSDSSLEDVKENSFRPHEISRFNSQSVFKSKKHHLKSIVVKCTLQLLMLNCLWELFHSDNMLTNIPKRKMVKLLDILKQSWEFAESFNSDFEIRAKILSSGIVEHMPNLLSQEALCAKLYFYTAFECMSSLKSDSHDTEEYNDLMDVFQKKIYLASQLVLHGFQRVIGDNPVKGVAAFQPVIAALVSYINSLDEIQFSRGKSEFYQLLCAIVACGHIDQQLGTSLSNAFLRYAC

Target ORF information:

RefSeq Version	NM_001020385.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative Sec7 domain-containing protein sec71 (sec71), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020385.2

ORF Insert Sequence:

ATGACAGATT TGGAGATAGA AACCGTTAGT AGGGTTTCTA GTAATCCTGA TGAAGTAAAA 
GGATCGTCTG TTGTCGAAGA AGAACCGGAT AGTGAATCAA CAATCAAAAG TCGAGTTTCT 
GATGAAATAG ATGAACATGA TTCCATACCT GAACAGTCTA ATACTGAAAA AAGTATAGAA 
ATTAACGATA AAAACTTAGA AGCGGAAAAA GATATTGAAA GCAATCTATC TCTAAGACCT 
CCAGAAGATG ACTTAGATTC ACGTTCGATT GAAAGTGAAC AAACAGGTAC TCTGAGCAAA 
CAGACTACAT CTACTTCTGA AGCTCCACAA GATCTAAAGA TTTGGAAGAA TATTTCAAAC 
GCCTCTAACC AAAACTCTGG CAAACTCAAT CCTCAACTCT TCGTAATTGA GGCTTTTAAG 
CATATGTCAA AAGCAAAGGC TACAAAACGG CATAAGAAGC TTAGGGAGGC TATCAATAAT 
GTCCAAATAG AATTACAAAA GCAGCCATTT CTTCTTCCCG AAGTCATTTT GGAACCTTTG 
GTAATGGCTT GTCAAACAAA TAGTACAACG TTGCTGACTA TTACTTTGGA TTGCTTTGCC 
AAGCTGATTG ATTACAATTA TTTTGATTCC CCAACATTAA ATCCATCGGA TATTACCTTA 
ATGGAACGTG TGGTTAACAC TATTGCATCC TGTTTTTGCG GTGAGTCTAC TCCAGAAAGA 
GTGCAGCTAC AAATTGTAAA GGCTTTATTG GCAGCTATTA CTTCTGAAAG GACAATCATT 
AGACATTCGT TTTTACTTAC AGCAGTGAGG CAAACTTACA ATATTTTCCT CTTATGTAAA 
GACTCAACTA CCCAAGCCAT TGCTCAGGTA GCTCTTTTAC AAATGGTTGA TTCAGTATTC 
CAGCGCCTTT CTACCGTTCT TAATCATGAA AGAGAATTTT CTACTATTAA CATGAATAAA 
TCATCCAGTA ATGGAACTCC TGATCGTGCT AACTCTCCTA TACCTTCTCA ATTATCAGAA 
AACAAACTCA CACTTGAATC TTTCGAGCAC AGAAAAAGCT TTGACCAGGT TCGTGAAGAA 
GCTCCTTTGG AAGAGGATTC TTTGGAGCAA CAGTTGCTTC GGGATGCATT TTTGTTAATC 
AGAGCTTTAT GTAAGCTTTC TATTAAAAAC ATTCCGTATG AGCATGAATA CGACCTAAAG 
TCGCAATCGA TGCGATCAAA GTTGATGAGC TTGCATCTTA TATATCATAT TCTTCGAACT 
TATATGAACA TCCTTTCGGA CATAAATGTG AAAATAAGGT CTCCAACTTC AACTCCGACA 
CCATTGATTG ACGCTGTTAA ACAATACATT TGCCTTGCAT TGGCTAAAAA TGTGGTTAGC 
CATGTTTTAC CGGTATTCGA GATTTCTTGT GAAATATTTT GGTTAATTCT TTCTGAATTA 
AAAAATTTTT TCAAGTCAGA GTTAGAAGTG TTTTTTACCG AAATCTTCTT TCCCATATTG 
GAAATGCGAA CCTCTAGCAA TCAACAAAAA ATTGTACTGC TGAATATTTT TCACCGCATG 
TGTGAAGAAC CACAGACCTT AATTGAACTT TATCTTAATT ATGACTGTAT AAGTGGTAAT 
ACTGAAAATA TTTATGAAAG AGCAATTGTT ACTCTATCAA GGATTGCAAG TCAGAGTACT 
TCTGATCCTC CTCCTTCATT TGTTTTCCGT GATGACCAGC TAGTAATTGA TAAACCGGGG 
TTTGTATATC ATACATTGAA TGATATTCCT CAGCTTAATT CGTCAACAAT TGGAAGCTAT 
GTTCATTCGC ACAATCCACC TTACTTTGAT TATCAAATTC GGTTGAAGTC TTATAGATGT 
TTAATATCTA CTTTATCTTC TCTATTTACA TGGTGTAATC AAACATTTGC GCCTACTGTC 
GAAATAACGG CGAAGGACGA CGAAACAGAG TCGACTTCAA AAGGAGAAGA ACCACAGAAA 
AGTAAATCAG AGCCCCCTTC CGCAGGTATA AATAGTACAT CAATGGATAA TTTGGAATCT 
TCTGGTCAGG CTTTAGCTAC AGACGATCCT TCTCAGTTTG AAAATTTAAA ACATCGAAAG 
AAACAATTAC AAGAAGCAAT ACAAAAATTT AACTACAAAC CAAAAGAGGG AATTAAGATT 
CTACTTTCAA GTCATTTTAT TGCTTCCAAA ACTCCTACCG ATATTGCTAA ATTTTTAATC 
TCTACAGAAG GCTTGGATAA AGCTGTGCTA GGCGAGTATT TAGGAGAAGG AAATGACGAG 
AATATTGCTA TCATGCACTC TTTCGTTGAC CACATGAGTT TCAATGATAT TCCATTTGTG 
AATGCCCTTC GTTCATTTTT ACAAAAATTT CGTTTACCCG GTGAAGCGCA AAAGATTGAC 
AGATTCATGC TCAAGTTTGC AGAAAAATAT ATTGATGACA ATCTTGGTGT TTTTAAAAAT 
GCAGACACTG CTTATATTCT AGCATATTCA ATTATTATGT TAAATACTGA TTTACATTCT 
CCCCAAGTTA AAAATCGGAT GACATGTCAA GATTTTATTA AAAATAACAG AGGTGTGGAT 
GATGGTGCTA ATCTGAGCGA TTCGTTTTTG ACTGAGGTAT ATGAGGAAAT CCAAAAAAAC 
GAAATTGTTT TGAAAGATGA GCAAGATCCT ACTTCCAACT TTCCAGAAAT ACCCGGGACT 
AGTAATCTTA GCTTTGCTGC TAACATAAGT AATGCATTGG CGACGGTTGG AAGAGATTTG 
CAAAGGGAAG CATACTATAT GGCTTCCAAT AAGATGGCCA ACAAGACCGA AGCGTTGTTT 
AAAGATTTAA TTCGTGAACA ACGTGAAAGA GGAAAACTTT CAGGAAATGA TATTTATTAT 
ACTGCTAGGC ACTTTGAGCA TGTTTGTCCG ATGTTTGAAG CTGTTTGGAT GCCAATTCTC 
GCGGCATTCT CTGAACCATT GCAACTCTCC TCTGATCCAG CATTGATTCA ACTTTCACTT 
GATGGATTTC GGTTGGCCAT GAACGTCATT TTCTTTTTCT CAATGGATTT ACCTAGAAAT 
GCTTTCATGC AAACTTTGAC TAAGTTTACT CATTTGAATA ATACGTCCGA GTTAAAATGG 
ACGAATATGC ACGCTTTGAA AACCCTACTT GAAATTTCAC TAGCTCATGG AGATAAGTTG 
AGAGACTCTT GGAAAGACGT GCTTTTATGT ATAAGTCAGT TGGAACGTGT CCAACTAATA 
TCTGCTGGTG TCGACATAAA TTCTTTACCA GATGTAAGTA CTACCAAACC ATTGCGCAAA 
TCTTTAGATA AGAATATACG ACAGTCTCGA AGTGGAAGCA TTTCGTTAAA ACATTCTAAA 
AGTTTTCAAT CTGCCTCCAC TCACTCCACT AAATCTAGCT CAGTTGAAAT CGTCAGAGAA 
TATAGTTCTC GTGAAGTTGT AATGGCTGTT GATATGCTCT TCTCAAACAC TCGAAACCTT 
GGTAGTGAAG GAATCTACGA TTTTGTAAAA GCATTAATTG AGGTCTCTTG GGAAGAAATA 
GAGTGTTCTT TGGAACTTTC TAATCCGAGG TTATTCAGTT TACAAAAGTT GGTTGAAATA 
TCATATTATA ATATGCGTCG TATCCGTATG GAATGGTCCA GTATTTGGTC TTTGTTAGGT 
ACTTATTTTA CCCAAGTCAG CTGTCATGAA AACTCAATAA TAGCATCTTT TGCTTTAGAT 
TCCTTGAGAC AATTTTCTAT GCAGTTTTTA GAAATTGAAG AGCTCTCTCA CTTTAAATTT 
CAGAAGGACT TCTTACAGCC ATTTTCACAT GCTATGGAAA ATTCGCAGGA CCTTAAGATT 
AAAGACCTTG TCTTACGTTG TATTGATCAA ATGATCAAAG CCAGATACCA AAACATACGT 
TCTGGATGGA GAACCATATT CCATATTCTC GCCTACGCTT CTAAAATTGA AAATCTTTTG 
GTTTTGCAGT GCGCAATATC TGTTGTATCA TCTTTGGGCC ATGAACATAT CAGCTGCGTC 
CTTACACAAG GGGCTTATAT TGATCTGATA TCTTGTATAA CTAAGTTTGC TAAACTTAAT 
GGCAATCAAA AGTTCTGTCT GAGTTGTGTG GATATGCTTA AAAATTTGGA GCATGAGTTA 
ATTAAGCATT TGAAGCACAT GAAAAAAGAG AGTGTATATT CAAAAAAACT AGAAGAAGAG 
TACTGGCTTC CGTTTTTGCT GTCATTTAAT GAAATCATCT GCGAGGCGTC AGATTTAGAG 
GTGCGATCTA AAGCCTTGAA GGTTTTATTC GATTGTCTTT ATCGACACGC TGATGACTTT 
GACGAAGAAT TTTGGGAAAC CGTTTCCAAT AAAGCTTTGC TCTCAATATT TTCTATTTTG 
TCCATTACTA ATTCCCAACG TTTGTATTTA GCAAAGAACA CTGAAGAGAC TGAAGTATGG 
ATGCTAACAA CAATGGTTGA AGCACTAAAA GCTTTTATTG AATTAATTAA AAACTTGTTT 
GAGCGCCTTC ATTTTTTGCT TCCTAAGGCG CTGAATTTAC TGGAAAAATG TATTTGCCAA 
GAAAACAGTA TGATATCAAA AGTGGGACTT AGTTGTTTTT CTCAATTTGT CCTCAAGAAT 
AAGAATCAAT TTAAAGACGT GGACTGGGAT GAAATAATTA ACTCGATAAA TCAGCTGCTT 
CAAATGACTC TTCCGATAGA ACTTCGTGAT CCTTCATTGT ATCCTCAAGT CAATTCTGAT 
AGTTCTCTTG AGGATGTCAA AGAAAATAGT TTTCGTCCAC ATGAAATATC AAGGTTTAAT 
AGCCAGAGCG TCTTTAAATC AAAAAAGCAC CATTTGAAAA GTATTGTTGT AAAGTGCACA 
TTACAACTTT TGATGCTTAA CTGCCTATGG GAATTATTTC ATTCTGACAA TATGCTTACC 
AATATTCCGA AAAGAAAAAT GGTAAAATTG CTGGATATCT TAAAGCAATC TTGGGAATTT 
GCTGAATCTT TTAATTCGGA TTTTGAAATA CGCGCTAAAA TCTTATCCTC GGGCATTGTG 
GAGCACATGC CCAATCTTTT AAGCCAAGAA GCCTTATGTG CTAAGCTATA TTTTTATACC 
GCATTTGAAT GTATGTCTTC TTTGAAGTCG GATTCCCATG ATACAGAAGA ATATAACGAT 
TTAATGGATG TTTTTCAGAA AAAAATTTAT CTTGCATCGC AATTGGTACT TCATGGTTTT 
CAAAGGGTAA TTGGTGATAA CCCTGTAAAG GGAGTTGCTG CATTCCAACC TGTAATAGCC 
GCCTTGGTTA GCTATATCAA TTCACTAGAC GAAATTCAAT TTTCTCGGGG AAAGTCCGAA 
TTCTATCAAT TACTGTGTGC CATTGTGGCT TGTGGACATA TTGACCAGCA ATTGGGAACT 
TCTCTAAGCA ACGCATTTTT AAGATATGCT TGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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sec71 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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