SPAC694.02 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAC694.02
Entrez Gene ID	2543333
Full Name	DEAD/DEAH box helicase
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001019911.2	NP_594482.1	DEAD/DEAH box helicase

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	SPAC694.02	NP_594482.1
Magnaporthe oryzae (rice blast fungus)	MGG_07250	XP_003715470.1
Neurospora crassa	NCU04472	XP_957041.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPAC694.02 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAC694.02 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc103369	NM_001019911.2 Latest version!	Schizosaccharomyces pombe DEAD/DEAH box helicase (SPAC694.02), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAC694.02 ( NM_001019911.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594482.1 Schizosaccharomyces pombe DEAD/DEAH box helicase (SPAC694.02), mRNA.

Price & Availability↑

CloneID	OSc103369
Clone ID Related Accession (Same CDS sequence)	NM_001019911.2
Accession Version	NM_001019911.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	DEAD/DEAH box helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019911.1.

ATGAGCGAGG AGATAGCCAA ATTAACGACG GTTCAGAACT ATCGTCTAGT AGACTTAATC 
GGTGATTTTG CTGGAGAGGA ACTTTTTCTT ATCGATGGAG ATTCCTTGAT TTTCCATATT 
TTGGCAAAAG ATTCTCAATT TTTAGCCAAG AATGGTGTTC AAATTTTATA TGCTATTTAC 
CTTTTGGAGA AGGAATTAGC GCGATTTAAG AGTGCTAGAC TAAATTTTTG TATAGTATTC 
TTTAAAAATT GGGAAGCTTT ATACTCCCAA TACGATGGGA TTTTGCATGC GTCGTTACTC 
CTCTTTCGTA GAGTGGCTAT CCGCCATCTT CGCAAGCATG TCCCTGTATA TGTTTTCAAA 
TCTTTAGGAG ATTCTCAATG GAGCATATTT ATTGCAAAGC ATCGTCCATA TGGATTTATG 
GTTCTTGATA GCAACCAAAA TACAAAGGAA GGGGGTTTAG TTATATCAGA TGAGCAAGTG 
ATGGTATTTA AATCATTCAT TTTCTTTGCT CTTTCTGATG GTGTACCTGT CGCTCTTTTG 
GGCTCTTACC AATTTGTAGA CAATAAAATC TTAGTCTGTA TATTGCCTTC ATTCTTTACA 
CGATCCAGTG AATCTGTTGA AAACGCTTCT AATCTCATCT CTGATTATTT AAAGAAGTTT 
GGCAAGCCTC GTCAGTCTAT TGAACTACCC GATATTGCTG TCGATGCTTG TCCTTCAAAC 
GCGTCACGCA TCCAAACCCT TTCTGCAGCG TGGTTGCTGT TGAATGAAAA ATCAGATCTT 
AATGTCGATC TCTGCAAAAT TCTCATCATC TTAACCTGCA TGCTTGAAGA ATTAAGCTTG 
AACGATCGCT TCCAACCTGC CTTCCCTCTT TATGAGGATC CTGCAATTCA GAAATTTATT 
ATTAGCTATC TGAATGCTAC AAATACAATC CTTCAGCTGT TGGATTCCAA AGTAGATGGA 
TTCCATGATA TTTTCGACGG TCGCCTCTTT ATCCGACTTG TTGTTGATGC TGCGTTGGGC 
GAATTTTGTA TTCCTGATAT TTGTCGAGAA TCCGCTCTTT CTTTATGGAA GCTTATTTTA 
GATATTGCTC CCGATGCAGG TGACTTGTTT GATTTTCCTA GCCTTTCTTC AATTTCCAAG 
GATCTGTGTG TACCCATTGA AACTCCTGTA AGCGAAATTG ATATATCTTT GACTGCCTAC 
AATGATCCCG TTATCTCCCC TTATTATGAT ACAGAGGAGT GGAATCTTGA ACAAAGGGAG 
GAACCTGGCA TTGAAACTGT TTTGGACTTC TCAAAACTTC GAATCAATGA CAAAACCATG 
ACATCCAAGC GCTTCGATGA TTTGAGTGCC ACTCGCAAAA CCGGTGATAA TACTAAAGAC 
CGCAAACAGG CGAATAAAGT ACGTAGACAA GATCAGTTCT TTATTAGTCA TATTCAAAAA 
TATGCAAGCT CTTTGACTGG TGCTAAAGGT GGTCAACTGA AACGTCAAAC CATTTTTACG 
CAAGGCCCCA ACTCTACAAA GGAGTCAAAG CAGGCAGTCA AAGAAGAAAC TAGTGTCAAA 
AATTCACCTA AAGGGAAAAA CACGAAAAAC GGTAAGGCTG AGAAGGAAAA GCCTGGAAAA 
AAGAGTAAAG CTCCTAAATT ATCCAAAGCC GAGCAAATTC GCGCTGAAAT TGCAGCACGA 
AAAGTTGAGA AAGAAGAGAA TAAAAACGAG GTTATTTGGA ATGAAACTAA AAATGATTTG 
GGTCGTATTA AAGATTTAAA CCTTCGTGCT GGCCGTCTTG CTCAGCTTTT GAATACAAGA 
ACGTATGACC CGTATATCGA GAACGAAATG CGTCTATATC GCATTCGTGT ATTACTTTCT 
TTATGGACCG CGTGCTGTGT AACCGATGAA GGAAAAGAGA AGAACATTAA GATTGCTGTC 
GAAATTTTCC GCGAAATAAG TATCGTTTAT CCAAATCCTA TTACGAAGCA AGTGAAAGCA 
GCTCTGGACG AAACACTTAC TGCGTTAGGA TTTGAAAACG TAATTAGAAA AATGAAAAAA 
TTAGAGTCGA AGCCTCTTTC GTTTCCATTT GTGTTACCTG ATTTGGAGGA TGAAGAATTT 
GACCTTGAGG TTCCCTATAC TTCTCCTACG TTCCAACTTC TCCATTTTGG TGAATACATG 
GAGAGGTCGA TGGGTAGTGC TCCTGATAGC CGTGTTGCAT TCGATCCGGA TGAATGGCAA 
CGTGAAACTC TTGACATACT GGATCGCGAT GAGTCTGTAT TTGTTGTAGC TCCAACATCT 
TCTGGTAAAA CCTTTATTTC GTTTTATGCG ATGGAAAAAG TTTTACGTGA CAATGATGAT 
GGAGTCGTTA TTTATGTTGC ACCTACTAAA GCATTAGTCA ATCAACTTTC TGCGGAAGTT 
TATGCTCGTT TCAACAAGCA TTATCCTCAT GCTGGTCAAA CTGTTTGGTC TGTTTATACT 
CGTGACTACC GTATTAATAA CCCCACAAAT TGTCAAGTTC TTATTACTGT TCCCCACGTG 
CTTCAAAGCA TGTTGTTGCA ACCAGCTTTG GCCAATGCAT GGTGCCCTAA GATTAGGCGC 
ATCATTTTTG ATGAAATTCA CTGTATTGGT CAAATGGAAG ATGGACTAGT TGAAGAACAG 
CTATTGTTAT TAGCTCCTTG TCCCATTGTT GCCCTATCAG CTACGGTCGG TAACCCACAG 
GAATTTCAAG TATGGCTTTC TGCTTTGCAA AGGGCTCACT CTTATCCAAT TCATTTGATT 
GTTCATGAGC ATCGTTATTC TGATCTTCGT AAGTTTGTAT ATGGAGGTGC CAAGGAATTT 
AATGGTTTGC AAGAAGAAGG CGACCTCGGA CAAATTGCAT TTATTCATCC TGCTGCCGCC 
ATGTCTTTCT CTGATGGATC TACAACGAAT CTTGCATTCG AACCACGCGA TTGCTTACAG 
CTGTACTATG CAATGAAGTT AGTTTCCAAG GGTGATTTCA AAATTAGTAG AAAGCTTGAT 
CCTGACAGGT ACTTTGAGGA CATCCCCTTT ATTAAGAAGG TTGATGCCGT CGGTTACGAG 
AAGAAGATTA AGGCTACAAT TGACACTTGG GCGTCTTTGC CTAATGCCTT TGCCCCTAAC 
TCACCATTCC AAGCTCTTAT TAAGCACTTT TGTAGTGAGC CACAAAAGGT TGTTAATAGT 
CAATTAGAAC TGCATAACTC TGCCTATTCT TTAGATGCCA TATCCCAAAA CCTTTTGCCA 
ATGTTAAAGG ATCTTCATAG TAAGGAGCTA CTTCCTGCTA TCTGTTTTAA CTATGATCGT 
CAGGAATGTG AATACCTTGC TGAAATCGTT TTTAAGACAT TGGTTGAGAA GGAGAGAGAG 
TGGCGTGAAA ATTCCGAGGA GTGGAAAGAG AAAATGCGTG AATATAAAAA ATGGCAATCC 
ACTGCTAACC AAAGAGCCAA ACAGATGGAA AAATTTGAGA AGAGTGCTAC TGAGAAACAA 
AAAGAACAAG CGTTAAAAGA AGCCGGTGAT ATCAGTTGGA TTGAGTTTTT CGACCCTGAG 
GAGCCTTCTC CCGAGTTTTC TTTTGCTGGT GCGAAGTCAA CCTATGGTAA AGAAGAACTT 
TATCGAGATT TAGAAGCCTT GCGTAGACGT CAAGCTGTTC CTGAATGGTA TGTTGATGCT 
TTATACCGTG GTATTGGTAT TCATCATTCT GGTCTTAACA GACGTTACCG TCAAATCGTC 
GAAGTTTTGT TTAGATGTGG TCAGCTTACA GTTGTAATTG CTACTCGTAC ACTCTCTCTT 
GGTATCAACA TGCCATGCCG AACTGTGGTA TTTTTGGGAG ATAGTTTACA GCTTAACGCC 
TTGAATTTCC ATCAAGCCGC TGGTCGTGCT GGTCGTCGTG GTTTTGATCT TCTAGGTCAT 
GTCGGTTTCT TGGGCGTACC TTTGCACAAA ATCTATCGTC TTTTGACTAC AAAGCTATGG 
GATATCCAGG GTCAATTTCC TCTTACTACT TCTTTGGTTT TGCAGCTATC TCAGCTTATT 
TCTGGGTCTC AAGCAGGCCC TTTTGCCCGT GATACCATTC ATGCGCTCAT GGACGAGCCA 
AAATTTATGG CTAATGGATC CATTTTGAGC AATCAAGTTA ACCATCATCT TCGCTTTGTC 
ATTGAGTACC TTCGTCGTGA AGGACTGTTA GATGTTTATG GTAACCCTAT AAATCTTGCT 
TCACTAACCA TGCATTTATA TTACACTGAA CCAAGCAATT ATGCATTCAT GAATCTTTTG 
AAAGCTGGTG TATTCCATAA AATTGCTGAG AAGTTTGCTA CTGAGAAATT CGAAGCCATG 
CGTGACACCG TCGTAATCTT GTGTCATCTT TTTGGTCGTA TTCGCATGAA CGAGCAATAC 
GTTATGACAT TGGAAGGAAG CACGGATCTT GCACAGTCTA CAGTTGTTTT ACCTCCTCTT 
CCAACGGAAA TTGCCGATGT TTTAGATGCC CATGATCAGC GAGTACTTGA CCTTTACAAG 
ACTTACGCTT GGTACTATCA AAAGCATGGT AATATTGGTG TGGCAGCAAA TAAGCTTCCA 
TTATCGAATA CCACCATTGA GGGTTCAGCC AAATTACCCT CTTCATTATC CGTAGGCGTA 
ATGTCCGAAT TTTGTGGGTT AGCAGGATAT AACGAGAGCA TGGTCGGTAC TACCTCAAGC 
TTCTTGGACA AAGTTCCAGA TGGCATTTTC TTGAAGACTT CTAGACTTCC AATATTTGAA 
GCCAACAAGG CCCCTTATAA TGCTTATTTA CTCGATTTCT TTACTCATGG AAGTGTCGAT 
ATGCTGATTG AACAAAATAA TTTGAAGCAG TCTGAAATTT GGTTTGTACT TAATGATTTC 
AGTTTGGTCC TTGCTACCAT CTGCAGTTGC CTTGGCAATC TATTAAATCT CGTAACTGAT 
GAAGATATTG AAAATGCTAT GAGTGCACTT GAAGGCTCCA ACGCTGTTCA AGCCACCACT 
GAAAGCGAGG CCACCGGAGC TGACTGGATG CAAAACATGA ATGGTAATTA TTCAACTACC 
TTGGCCTCTC CTGAATTGGA GGAGAAAATG GACATGAATC TTTTCAAGGT ATACTGCATG 
TTTTTGGAAG TACGTAATCA ATTTGACGCT AAATTCAGAA AGATGTGGGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594482.1
CDS	38..5191
Translation	MSEEIAKLTTVQNYRLVDLIGDFAGEELFLIDGDSLIFHILAKDSQFLAKNGVQILYAIYLLEKELARFKSARLNFCIVFFKNWEALYSQYDGILHASLLLFRRVAIRHLRKHVPVYVFKSLGDSQWSIFIAKHRPYGFMVLDSNQNTKEGGLVISDEQVMVFKSFIFFALSDGVPVALLGSYQFVDNKILVCILPSFFTRSSESVENASNLISDYLKKFGKPRQSIELPDIAVDACPSNASRIQTLSAAWLLLNEKSDLNVDLCKILIILTCMLEELSLNDRFQPAFPLYEDPAIQKFIISYLNATNTILQLLDSKVDGFHDIFDGRLFIRLVVDAALGEFCIPDICRESALSLWKLILDIAPDAGDLFDFPSLSSISKDLCVPIETPVSEIDISLTAYNDPVISPYYDTEEWNLEQREEPGIETVLDFSKLRINDKTMTSKRFDDLSATRKTGDNTKDRKQANKVRRQDQFFISHIQKYASSLTGAKGGQLKRQTIFTQGPNSTKESKQAVKEETSVKNSPKGKNTKNGKAEKEKPGKKSKAPKLSKAEQIRAEIAARKVEKEENKNEVIWNETKNDLGRIKDLNLRAGRLAQLLNTRTYDPYIENEMRLYRIRVLLSLWTACCVTDEGKEKNIKIAVEIFREISIVYPNPITKQVKAALDETLTALGFENVIRKMKKLESKPLSFPFVLPDLEDEEFDLEVPYTSPTFQLLHFGEYMERSMGSAPDSRVAFDPDEWQRETLDILDRDESVFVVAPTSSGKTFISFYAMEKVLRDNDDGVVIYVAPTKALVNQLSAEVYARFNKHYPHAGQTVWSVYTRDYRINNPTNCQVLITVPHVLQSMLLQPALANAWCPKIRRIIFDEIHCIGQMEDGLVEEQLLLLAPCPIVALSATVGNPQEFQVWLSALQRAHSYPIHLIVHEHRYSDLRKFVYGGAKEFNGLQEEGDLGQIAFIHPAAAMSFSDGSTTNLAFEPRDCLQLYYAMKLVSKGDFKISRKLDPDRYFEDIPFIKKVDAVGYEKKIKATIDTWASLPNAFAPNSPFQALIKHFCSEPQKVVNSQLELHNSAYSLDAISQNLLPMLKDLHSKELLPAICFNYDRQECEYLAEIVFKTLVEKEREWRENSEEWKEKMREYKKWQSTANQRAKQMEKFEKSATEKQKEQALKEAGDISWIEFFDPEEPSPEFSFAGAKSTYGKEELYRDLEALRRRQAVPEWYVDALYRGIGIHHSGLNRRYRQIVEVLFRCGQLTVVIATRTLSLGINMPCRTVVFLGDSLQLNALNFHQAAGRAGRRGFDLLGHVGFLGVPLHKIYRLLTTKLWDIQGQFPLTTSLVLQLSQLISGSQAGPFARDTIHALMDEPKFMANGSILSNQVNHHLRFVIEYLRREGLLDVYGNPINLASLTMHLYYTEPSNYAFMNLLKAGVFHKIAEKFATEKFEAMRDTVVILCHLFGRIRMNEQYVMTLEGSTDLAQSTVVLPPLPTEIADVLDAHDQRVLDLYKTYAWYYQKHGNIGVAANKLPLSNTTIEGSAKLPSSLSVGVMSEFCGLAGYNESMVGTTSSFLDKVPDGIFLKTSRLPIFEANKAPYNAYLLDFFTHGSVDMLIEQNNLKQSEIWFVLNDFSLVLATICSCLGNLLNLVTDEDIENAMSALEGSNAVQATTESEATGADWMQNMNGNYSTTLASPELEEKMDMNLFKVYCMFLEVRNQFDAKFRKMWA

Target ORF information:

RefSeq Version	NM_001019911.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe DEAD/DEAH box helicase (SPAC694.02), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001019911.2

ORF Insert Sequence:

ATGAGCGAGG AGATAGCCAA ATTAACGACG GTTCAGAACT ATCGTCTAGT AGACTTAATC 
GGTGATTTTG CTGGAGAGGA ACTTTTTCTT ATCGATGGAG ATTCCTTGAT TTTCCATATT 
TTGGCAAAAG ATTCTCAATT TTTAGCCAAG AATGGTGTTC AAATTTTATA TGCTATTTAC 
CTTTTGGAGA AGGAATTAGC GCGATTTAAG AGTGCTAGAC TAAATTTTTG TATAGTATTC 
TTTAAAAATT GGGAAGCTTT ATACTCCCAA TACGATGGGA TTTTGCATGC GTCGTTACTC 
CTCTTTCGTA GAGTGGCTAT CCGCCATCTT CGCAAGCATG TCCCTGTATA TGTTTTCAAA 
TCTTTAGGAG ATTCTCAATG GAGCATATTT ATTGCAAAGC ATCGTCCATA TGGATTTATG 
GTTCTTGATA GCAACCAAAA TACAAAGGAA GGGGGTTTAG TTATATCAGA TGAGCAAGTG 
ATGGTATTTA AATCATTCAT TTTCTTTGCT CTTTCTGATG GTGTACCTGT CGCTCTTTTG 
GGCTCTTACC AATTTGTAGA CAATAAAATC TTAGTCTGTA TATTGCCTTC ATTCTTTACA 
CGATCCAGTG AATCTGTTGA AAACGCTTCT AATCTCATCT CTGATTATTT AAAGAAGTTT 
GGCAAGCCTC GTCAGTCTAT TGAACTACCC GATATTGCTG TCGATGCTTG TCCTTCAAAC 
GCGTCACGCA TCCAAACCCT TTCTGCAGCG TGGTTGCTGT TGAATGAAAA ATCAGATCTT 
AATGTCGATC TCTGCAAAAT TCTCATCATC TTAACCTGCA TGCTTGAAGA ATTAAGCTTG 
AACGATCGCT TCCAACCTGC CTTCCCTCTT TATGAGGATC CTGCAATTCA GAAATTTATT 
ATTAGCTATC TGAATGCTAC AAATACAATC CTTCAGCTGT TGGATTCCAA AGTAGATGGA 
TTCCATGATA TTTTCGACGG TCGCCTCTTT ATCCGACTTG TTGTTGATGC TGCGTTGGGC 
GAATTTTGTA TTCCTGATAT TTGTCGAGAA TCCGCTCTTT CTTTATGGAA GCTTATTTTA 
GATATTGCTC CCGATGCAGG TGACTTGTTT GATTTTCCTA GCCTTTCTTC AATTTCCAAG 
GATCTGTGTG TACCCATTGA AACTCCTGTA AGCGAAATTG ATATATCTTT GACTGCCTAC 
AATGATCCCG TTATCTCCCC TTATTATGAT ACAGAGGAGT GGAATCTTGA ACAAAGGGAG 
GAACCTGGCA TTGAAACTGT TTTGGACTTC TCAAAACTTC GAATCAATGA CAAAACCATG 
ACATCCAAGC GCTTCGATGA TTTGAGTGCC ACTCGCAAAA CCGGTGATAA TACTAAAGAC 
CGCAAACAGG CGAATAAAGT ACGTAGACAA GATCAGTTCT TTATTAGTCA TATTCAAAAA 
TATGCAAGCT CTTTGACTGG TGCTAAAGGT GGTCAACTGA AACGTCAAAC CATTTTTACG 
CAAGGCCCCA ACTCTACAAA GGAGTCAAAG CAGGCAGTCA AAGAAGAAAC TAGTGTCAAA 
AATTCACCTA AAGGGAAAAA CACGAAAAAC GGTAAGGCTG AGAAGGAAAA GCCTGGAAAA 
AAGAGTAAAG CTCCTAAATT ATCCAAAGCC GAGCAAATTC GCGCTGAAAT TGCAGCACGA 
AAAGTTGAGA AAGAAGAGAA TAAAAACGAG GTTATTTGGA ATGAAACTAA AAATGATTTG 
GGTCGTATTA AAGATTTAAA CCTTCGTGCT GGCCGTCTTG CTCAGCTTTT GAATACAAGA 
ACGTATGACC CGTATATCGA GAACGAAATG CGTCTATATC GCATTCGTGT ATTACTTTCT 
TTATGGACCG CGTGCTGTGT AACCGATGAA GGAAAAGAGA AGAACATTAA GATTGCTGTC 
GAAATTTTCC GCGAAATAAG TATCGTTTAT CCAAATCCTA TTACGAAGCA AGTGAAAGCA 
GCTCTGGACG AAACACTTAC TGCGTTAGGA TTTGAAAACG TAATTAGAAA AATGAAAAAA 
TTAGAGTCGA AGCCTCTTTC GTTTCCATTT GTGTTACCTG ATTTGGAGGA TGAAGAATTT 
GACCTTGAGG TTCCCTATAC TTCTCCTACG TTCCAACTTC TCCATTTTGG TGAATACATG 
GAGAGGTCGA TGGGTAGTGC TCCTGATAGC CGTGTTGCAT TCGATCCGGA TGAATGGCAA 
CGTGAAACTC TTGACATACT GGATCGCGAT GAGTCTGTAT TTGTTGTAGC TCCAACATCT 
TCTGGTAAAA CCTTTATTTC GTTTTATGCG ATGGAAAAAG TTTTACGTGA CAATGATGAT 
GGAGTCGTTA TTTATGTTGC ACCTACTAAA GCATTAGTCA ATCAACTTTC TGCGGAAGTT 
TATGCTCGTT TCAACAAGCA TTATCCTCAT GCTGGTCAAA CTGTTTGGTC TGTTTATACT 
CGTGACTACC GTATTAATAA CCCCACAAAT TGTCAAGTTC TTATTACTGT TCCCCACGTG 
CTTCAAAGCA TGTTGTTGCA ACCAGCTTTG GCCAATGCAT GGTGCCCTAA GATTAGGCGC 
ATCATTTTTG ATGAAATTCA CTGTATTGGT CAAATGGAAG ATGGACTAGT TGAAGAACAG 
CTATTGTTAT TAGCTCCTTG TCCCATTGTT GCCCTATCAG CTACGGTCGG TAACCCACAG 
GAATTTCAAG TATGGCTTTC TGCTTTGCAA AGGGCTCACT CTTATCCAAT TCATTTGATT 
GTTCATGAGC ATCGTTATTC TGATCTTCGT AAGTTTGTAT ATGGAGGTGC CAAGGAATTT 
AATGGTTTGC AAGAAGAAGG CGACCTCGGA CAAATTGCAT TTATTCATCC TGCTGCCGCC 
ATGTCTTTCT CTGATGGATC TACAACGAAT CTTGCATTCG AACCACGCGA TTGCTTACAG 
CTGTACTATG CAATGAAGTT AGTTTCCAAG GGTGATTTCA AAATTAGTAG AAAGCTTGAT 
CCTGACAGGT ACTTTGAGGA CATCCCCTTT ATTAAGAAGG TTGATGCCGT CGGTTACGAG 
AAGAAGATTA AGGCTACAAT TGACACTTGG GCGTCTTTGC CTAATGCCTT TGCCCCTAAC 
TCACCATTCC AAGCTCTTAT TAAGCACTTT TGTAGTGAGC CACAAAAGGT TGTTAATAGT 
CAATTAGAAC TGCATAACTC TGCCTATTCT TTAGATGCCA TATCCCAAAA CCTTTTGCCA 
ATGTTAAAGG ATCTTCATAG TAAGGAGCTA CTTCCTGCTA TCTGTTTTAA CTATGATCGT 
CAGGAATGTG AATACCTTGC TGAAATCGTT TTTAAGACAT TGGTTGAGAA GGAGAGAGAG 
TGGCGTGAAA ATTCCGAGGA GTGGAAAGAG AAAATGCGTG AATATAAAAA ATGGCAATCC 
ACTGCTAACC AAAGAGCCAA ACAGATGGAA AAATTTGAGA AGAGTGCTAC TGAGAAACAA 
AAAGAACAAG CGTTAAAAGA AGCCGGTGAT ATCAGTTGGA TTGAGTTTTT CGACCCTGAG 
GAGCCTTCTC CCGAGTTTTC TTTTGCTGGT GCGAAGTCAA CCTATGGTAA AGAAGAACTT 
TATCGAGATT TAGAAGCCTT GCGTAGACGT CAAGCTGTTC CTGAATGGTA TGTTGATGCT 
TTATACCGTG GTATTGGTAT TCATCATTCT GGTCTTAACA GACGTTACCG TCAAATCGTC 
GAAGTTTTGT TTAGATGTGG TCAGCTTACA GTTGTAATTG CTACTCGTAC ACTCTCTCTT 
GGTATCAACA TGCCATGCCG AACTGTGGTA TTTTTGGGAG ATAGTTTACA GCTTAACGCC 
TTGAATTTCC ATCAAGCCGC TGGTCGTGCT GGTCGTCGTG GTTTTGATCT TCTAGGTCAT 
GTCGGTTTCT TGGGCGTACC TTTGCACAAA ATCTATCGTC TTTTGACTAC AAAGCTATGG 
GATATCCAGG GTCAATTTCC TCTTACTACT TCTTTGGTTT TGCAGCTATC TCAGCTTATT 
TCTGGGTCTC AAGCAGGCCC TTTTGCCCGT GATACCATTC ATGCGCTCAT GGACGAGCCA 
AAATTTATGG CTAATGGATC CATTTTGAGC AATCAAGTTA ACCATCATCT TCGCTTTGTC 
ATTGAGTACC TTCGTCGTGA AGGACTGTTA GATGTTTATG GTAACCCTAT AAATCTTGCT 
TCACTAACCA TGCATTTATA TTACACTGAA CCAAGCAATT ATGCATTCAT GAATCTTTTG 
AAAGCTGGTG TATTCCATAA AATTGCTGAG AAGTTTGCTA CTGAGAAATT CGAAGCCATG 
CGTGACACCG TCGTAATCTT GTGTCATCTT TTTGGTCGTA TTCGCATGAA CGAGCAATAC 
GTTATGACAT TGGAAGGAAG CACGGATCTT GCACAGTCTA CAGTTGTTTT ACCTCCTCTT 
CCAACGGAAA TTGCCGATGT TTTAGATGCC CATGATCAGC GAGTACTTGA CCTTTACAAG 
ACTTACGCTT GGTACTATCA AAAGCATGGT AATATTGGTG TGGCAGCAAA TAAGCTTCCA 
TTATCGAATA CCACCATTGA GGGTTCAGCC AAATTACCCT CTTCATTATC CGTAGGCGTA 
ATGTCCGAAT TTTGTGGGTT AGCAGGATAT AACGAGAGCA TGGTCGGTAC TACCTCAAGC 
TTCTTGGACA AAGTTCCAGA TGGCATTTTC TTGAAGACTT CTAGACTTCC AATATTTGAA 
GCCAACAAGG CCCCTTATAA TGCTTATTTA CTCGATTTCT TTACTCATGG AAGTGTCGAT 
ATGCTGATTG AACAAAATAA TTTGAAGCAG TCTGAAATTT GGTTTGTACT TAATGATTTC 
AGTTTGGTCC TTGCTACCAT CTGCAGTTGC CTTGGCAATC TATTAAATCT CGTAACTGAT 
GAAGATATTG AAAATGCTAT GAGTGCACTT GAAGGCTCCA ACGCTGTTCA AGCCACCACT 
GAAAGCGAGG CCACCGGAGC TGACTGGATG CAAAACATGA ATGGTAATTA TTCAACTACC 
TTGGCCTCTC CTGAATTGGA GGAGAAAATG GACATGAATC TTTTCAAGGT ATACTGCATG 
TTTTTGGAAG TACGTAATCA ATTTGACGCT AAATTCAGAA AGATGTGGGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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SPAC694.02 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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