sec16 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	sec16
Entrez Gene ID	2542678
Full Name	multidomain vesicle coat component Sec16 (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001020416.2	NP_594985.1	putative multidomain vesicle coat component Sec16

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following sec16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sec16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102131	NM_001020416.2 Latest version!	Schizosaccharomyces pombe putative multidomain vesicle coat component Sec16 (sec16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sec16 ( NM_001020416.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594985.1 Schizosaccharomyces pombe putative multidomain vesicle coat component Sec16 (sec16), mRNA.

Price & Availability↑

CloneID	OSc102131
Clone ID Related Accession (Same CDS sequence)	NM_001020416.2
Accession Version	NM_001020416.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative multidomain vesicle coat component Sec16
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001020416.1.

ATGGATATTG AATCAGACCA CGAGCCTAAG GAGTCTTCTG TTGATCTTGA AGGACGGCAT 
GATTCTATAC GCAAGTCTTT TGAAGCTGAC GATATTGATG TGACAGATGA AAACACCATT 
TTTTCTACCT CTAGAAAAGA ACAAAGCACA ATTGAACCTA TGGATTCCTT TGTTGAAAAC 
AACATAGCCA ATAGCTCCTC TCAACAGTCA GTTTCAGAAA TTGACATTCA GTTGCCTTCC 
TCTGAGCCTA AAGAAGGTGT TAAAGAAGAA AGCAACTTAA TAGAGAGTAA TTCTTTGGAC 
AATGTGCAGG AAATTAATCC TCAAAATACC ATTGGCGACA AATCGGCTCA CACAGAAGAG 
GAGTCTTCAA ATGATAACTT GGTAAAAATT ACTGCTGGAA CCATTATTTC TGACATAATG 
GATGAGCCTG TTGTGGATGA AGACGATAAA CTCTCTGAAG AAGACTTGAG CAAGGAAGAC 
TTAGAAACGA CTCTTAAGGG TGACGAAAAC TATTCATCGT CGCAAAAAGA AATATATGAT 
AATGATGAGA CAATTCCTAA ACCACCATCT CCAGAAGTCG AAAAGATAGA AACTAGTACT 
TCAGTTGAGA ATGCATACAT TACGAACTCT CAATATAATG ACACTCTTGA TATGGAAGAT 
CAGACATCTG ATTTAATTAC AACTTTTGAG CATGAAAATG AAGACCATGA AGTCCACGAG 
GTTCCTTCCA TTCACGGAAT GGAAGGGACT TTTGAAACCG TAAATCTAAC TTCTGAAGCT 
GGGAATGAAA AAGAGTTTAA CAACGATTCT GTAGTATTGA ATACGACGCC AAAAGAAATG 
CAAATTTCAT CTTTAGATGC TGTTGACAAA CTTGAAGATA AGCCTGTTTC GAACGACAAT 
ATCAAAGCCA ATATAGAATC GTCGACTACA GTTGATAGGG CTTCATCTCA GCTTGAATCG 
GAAAATAACG AATCCACTTT TTTTGTACAT AACCAAAATT CCTCCGGGCA ACATCAAACT 
TTGGATTTCG TCGCAAGCAA ACCCGTTACC GATACCCCTG AGCTTAGTAA GGAAAATACG 
TTGGTGTCTT CTGAAAACCT AAATGATCCC AAACCAAATT TGCCGGAGAC TGAGGTTGAT 
TTTGGAGAGC CTTTGGAAGT TGAGGCTCCC AGTTTTGTGG TCCAGAATAA CAGTGCTGTG 
CAGCCAACGA CCCAGAAAAC TTCCGAAGAT TCAACCGATC TACACACCAA TGAGATCCCA 
AATGTTGCCC AACCTCCTTC TTCTTTTGAA AAGGAGAATA ACAAAGATAC TAGCAAGTTG 
GAAAATCCGG ATATTTCTTC ATCACCATTA AGTCCAACTG AAGATTTGTT TCCTAATGAC 
CCAGAAGAAG AAAATTTATT TTCGGCGGCT TTGGGCTTGA ATTCGAATAC TGGTTCGCAG 
CCATCAGAAA CCCAGAGTAA GCCATCAATC GATCCATCTG AATCAATAAC TGTAACCGAT 
AATCAAGATT CTCTTCTCTT TTCCCAACTC ACTAATAATG CTTTGCAAGC AGAAAATGCT 
ACGAAAGTAT CCGAAAACAC TATCAATGAT GAGGAGTTGA TAGATGATTC AGAATTTACT 
TCGCTTATGA GCAACTTTTT GGAAAGCAGT ACTGTTCAAA CAAATAAGTA TCTCCCAAAA 
TCATCAGCAT CTCCGCCAGC CAATGCTCCA ATTGTTTCTT CGGATGTTCA TAAAAATGAA 
AATGCTGGTA CAGCGCGTAG AGCTCCTCAA TCTGGAGCAT TCGTTGCATC AAAGGCGAAA 
TATTCTTCTC CTTATGATTT GCCCGAAGAA ATAGTACAAG TAGCACAGCA AAAAAGGTCT 
GTATCACAAA ATTACAACCG GCAGTACTCT TTTCAACCTC GTCCTGCTAC TCCATCCAAT 
CCCCCTCGTT CTTTACCTCC TCCTAGTGGT CAGGTGAATG CCCCAATGAG TCAAACCCCT 
AATCCAATTT CGTTTGCTTA CCAGCATGGT ACTCCTTTGG CGACACCTAC CATGCGCGCT 
AACTCTTTCA ATTCCTATCC AGCGTCTAGT GCTGAACCGA TCAGGAGACC GGCAACCACC 
ACCGTTGGAC ACACTCCTAA CTTGTACAGC CCAAAGACTA ATACGTACAA TTCTCGACAT 
ATGGCATATG AAATGACAAA ATCTCATATT AACGTTATTT CTCCGGGACC TTCTTTACAA 
GTTAATGCTC CATACACGCC GACTTCTGGC GAGCTTGGTA ATAAAGTTAG CAACCCTACC 
AAGGAATTCG TATCGACATC GTCTTATGCA CCTGCTGCCA ACACAAGAAA TGCTATTATT 
CGTGAACCCG GTATTCTATC ACCTCTTTCT CCTCGTGTAC AGCCTGTACT GTCTCGACGA 
GAAAGCATTA TTTCGATGGG TTCATCTGCC AGTAGTTATG TTCCTTTGGA AATAGCACCA 
AGACCGATGT CTTCGCTTGA GCATACTATG AATTCTGCGA TGTCTCCGGG TAATTTGCAG 
AGAACCGCAA ACCTGTACAA ACCAATGACG ACTCCCAATG CATATAATAT CAAAAATTCC 
AACCAGAGGG AAACAAAATA TCCTTATCAG CCGCAAGCTA TAAATTACTC TGAGGTTACC 
CAGCCTGGTA GTTCTTCGTT ACCGACAAGC GGAGAAGAAG CGAACATTAT CAGAAGTCCA 
GGATTTACAC CTTTAGCAGC ACAAAAGGAT GCAACCATAT ATACCCCTTC TCACGCACAA 
GCAACTTTAT ACGGTAATAT GGATAATAAC GACAGAGATA ATGAGGGTAT CCATGACATA 
TTACAATCAG ATATGGAACC TGTATTACCC CCTCATAACT CTGCTTACCA TGCTAATGCT 
CCCGTAAGTT CTCATTCAGA AGGACTGAAC AATAGACTTC CGATTTCACC CCTTCCACCC 
CAATTACACA AAACTGGCAC ACCTTCACAT CAACATGGAT TTGACACTGC TGAAACTACG 
GCGAAGCAAT ATGCACCATC TATTCCACCG AATTTTAATC CAAACGTAAG CATAGATACA 
ATGACTGAAG GGGTTGCGCT CCCTTCGGCT ACTCTTGATT CTGATAAAAG CTCGTTGCAT 
AAACGTAGCG CTGAATTATC ACGAAATAAT TCTCCCCGCC CTGATTTCTT ACCATTACCA 
AATCAGCCAC TTTTGCATTC TAATTTGCAT TCGCCTGTCT CGCCAGTTGT TGATAGTAAT 
GAAGACTCTC GACTTAAATT TTTAAGTACG CAAAGACCTG CATTTTCGTT TGGACCTTGT 
GGTACAATCG TTATGGCGTT TTCCACTCCC AGTGGTTTAT ACACAACTAG TGGCAAAGGT 
ACCAAATTCA TTGCAGGACC CATTAAAATA GAAAAATTGG GTGATGTGCT AACGGATGAG 
TATCGTCATC TAAAGGAGTT TAAAGGACCA TACCTTGCCT CTAATGGAAA GGTAGATAAA 
CATGGAAAAG CCGAAGCTAT TGAATGGCTT TCCAAATATA TTGACCGTCT AAACCAATCG 
CTAGAATACG ACGATAAAAA CATTACGCTT AAGGACAAGT TACTTTTACT ACAGTGTTTG 
AAGATGCTAC TGGAAGTGAG TGATCGTAAG CTAATAGTTG AAAAGCTTCG TCCTATCTTA 
TTACCTTCCT TTGAAATTCC TGAACCTTGC AATACAGCTA CTTCAGTTCA AGAGCTTATT 
AATCCTGAAA TTAATCAGGA TGATTCTCCA ATTGTCGCAT CTCGTTATTG CACAACTTCT 
TTCCTTCACC GATTTTATGA ATATTTATTG TCTGGCAACA AAGATGAAGC GCTTACTTAT 
GCTTTGCAGC AGAAGCAATG GCCATATGCG ATTATTGTTG CGCATTCTAT AGATGCAAAA 
ACATTTCAGG GAGTAGTACG AACGTTTTGT AAATCAGAGG TCAAAGAATC TATGCTTAGA 
TCTGGTGTTG GAGTAAATTT ACAATTATCT TTGCAATTAA TGTCTGATGC CCATGCGTCT 
TCAATGTCAG AGTTTTCGTC TTCTACGTCA CTATTGAATT TGGCGGATCA ATCGCAGGCA 
AGTAATGCTT TGGTAGCCTG GAAGGAGTTG CTGTACAATA TTATTGCAAA TCATTACTCT 
GACCAAAAAG AAGCATTGAG GGTTTTAGGG ACATTACTAC TCCAGGAAAA CCGTGTTTAT 
GCGGCACATC TGGTTTACAT CTTAAGTTTA TCACCGGATG TTTGCTCGAA CAAATCGAAT 
TCTCTTTTTG AACTTGTTGG TTTAAGTAAA CATAACTTAT ATCCTTCGCA TGATGATTTA 
TTTGACGTTA CACAATTGAC CGAAGTATTG GAGTTGGTAT TCAACGTCTA TTCTGAAAAG 
ACACCTGTTT TTTTTACTCA CTTAGTACCA TATCGCTTGT ATGAAGCTGA AGTTTTGGCT 
GAAGCTGGTG AGGTATCTGC TGCAAGAAAA TACTGTGAAC TAATCGGAAA CTACCTAAAT 
CGTGTTGCAA AGAAGTCAAA CAATGTTGAT CCAGGTTTCG TTCTTCGTGT TCGCGATTTG 
ACCCAGCAAA TATTGGAGAA TAGTGCAGGC AGTGAAGATA TCTCTTCGTC TTGGCTTGGT 
AGGACAGTGT CTAGACCTAG ACTGGATACA GTTTTATCGT CGTTGGGTAG CAAATTTAGT 
AAATTCGTAG CTGGAGATCC CAACTTTGAT GTTATGCGTC CAGCTACGGT TGGGCCGGGA 
CCATTTGGAA AGGTTGCCAG TCAAAAGAAT TTAACAGTTC AAACAAATAC TAACAATGCT 
GCCATGGAAT CATTTTATTC CGACAGACCT ACGTCTTCTG GACCTTCCTA CCAAAACCGA 
ACGCCTTTAA CAGGCCAGGA GTCAATGAAT ATGGGAGTTT ATTCACCTTA TCGTCGTAGC 
ACAGAAATTG CTGAGAATAT GAGTATGGAT GGCAATGCCT ATCCTTATAC TCCAGCTTCT 
CAAGAGAATC CCTATACCCC GCGTCATTCT CAAGAAGACA ATGCATCTGT ATTGTCACAA 
CAACCCTTAA CATTTTATTC AAATGTCGCA GACAATTCAT ATATGCCTGT GTCTGCATCA 
CAAGAACCGA AGATGGGTAT GGGAACTGCA TTTAATATGC CGACAAATGA GGTTCATGGT 
GTTGGAGCAG AGATGGCATC GCCTTATCAA CCACTTCAAC CGGCATCAGC TCATCTACCA 
AACTTACAAC CTACCCTGGC ACCCATCAAT CAAAATGCCT ATGTACCTAG TAATATTGCT 
CCGGCGATGG GGGCAATGCA AAGTGCACCG AGTGCAGAAG CAGTGGCCGC ACCATCTGAG 
AGTTTGAATC TCAATAAAGA TCGAAGTCAG CAAGCCAAAC AAGCAGCAGC ACAAAACGTG 
GCAGACTTGG TTCGTCAGGA AGAAGAGAAG GAAAAGCAAA AGCAAAAAGC AAAGAAGAAT 
GCAGAATCTG GTAAAAAAGG AGGAGCAAAG GGATGGTTCT CAAAATTGTT AAGACGGGAT 
GAGTCAAAGG ATCAACCCAC CGTGTATAAA GCGAAATTAG GGGAGAAATC CCATTTGCAT 
TATGATAAAG AATTGAAACG ATGGGTCAAT GATGACGGCA GTGATTTGAG TAATCAAGCA 
GCACCGCCTC CACCACCTCC CATGGCGCTT CCCAAAGCCG GTCCTCCTTC CGCAGCACCT 
ACAAGCGCTC TACCTCCTGC TGGTCCACCT GCAGGTGCTA CAGCAATTTC CGGGAATCCA 
GGAATGCCGG CGCCTGTACC GCTTACTGGT AAGGAGACCG CTGTTCCTCT ATCCTCTATG 
CCGAATGCGC CTCCCAGCGT TGCTAGCAAT GCCAAGTTAC CACCGGCTAG CAACAATCGA 
AAAGTTGATC CATTAGAAGA TATTTTACAA GCGATGCCAC CTCCGACAAC ACGTAAGGCT 
AGAGGGAAAA CAAGTAAAAG ATATGTGGAT GTCATGAGAA ATAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594985.1
CDS	265..6252
Translation	MDIESDHEPKESSVDLEGRHDSIRKSFEADDIDVTDENTIFSTSRKEQSTIEPMDSFVENNIANSSSQQSVSEIDIQLPSSEPKEGVKEESNLIESNSLDNVQEINPQNTIGDKSAHTEEESSNDNLVKITAGTIISDIMDEPVVDEDDKLSEEDLSKEDLETTLKGDENYSSSQKEIYDNDETIPKPPSPEVEKIETSTSVENAYITNSQYNDTLDMEDQTSDLITTFEHENEDHEVHEVPSIHGMEGTFETVNLTSEAGNEKEFNNDSVVLNTTPKEMQISSLDAVDKLEDKPVSNDNIKANIESSTTVDRASSQLESENNESTFFVHNQNSSGQHQTLDFVASKPVTDTPELSKENTLVSSENLNDPKPNLPETEVDFGEPLEVEAPSFVVQNNSAVQPTTQKTSEDSTDLHTNEIPNVAQPPSSFEKENNKDTSKLENPDISSSPLSPTEDLFPNDPEEENLFSAALGLNSNTGSQPSETQSKPSIDPSESITVTDNQDSLLFSQLTNNALQAENATKVSENTINDEELIDDSEFTSLMSNFLESSTVQTNKYLPKSSASPPANAPIVSSDVHKNENAGTARRAPQSGAFVASKAKYSSPYDLPEEIVQVAQQKRSVSQNYNRQYSFQPRPATPSNPPRSLPPPSGQVNAPMSQTPNPISFAYQHGTPLATPTMRANSFNSYPASSAEPIRRPATTTVGHTPNLYSPKTNTYNSRHMAYEMTKSHINVISPGPSLQVNAPYTPTSGELGNKVSNPTKEFVSTSSYAPAANTRNAIIREPGILSPLSPRVQPVLSRRESIISMGSSASSYVPLEIAPRPMSSLEHTMNSAMSPGNLQRTANLYKPMTTPNAYNIKNSNQRETKYPYQPQAINYSEVTQPGSSSLPTSGEEANIIRSPGFTPLAAQKDATIYTPSHAQATLYGNMDNNDRDNEGIHDILQSDMEPVLPPHNSAYHANAPVSSHSEGLNNRLPISPLPPQLHKTGTPSHQHGFDTAETTAKQYAPSIPPNFNPNVSIDTMTEGVALPSATLDSDKSSLHKRSAELSRNNSPRPDFLPLPNQPLLHSNLHSPVSPVVDSNEDSRLKFLSTQRPAFSFGPCGTIVMAFSTPSGLYTTSGKGTKFIAGPIKIEKLGDVLTDEYRHLKEFKGPYLASNGKVDKHGKAEAIEWLSKYIDRLNQSLEYDDKNITLKDKLLLLQCLKMLLEVSDRKLIVEKLRPILLPSFEIPEPCNTATSVQELINPEINQDDSPIVASRYCTTSFLHRFYEYLLSGNKDEALTYALQQKQWPYAIIVAHSIDAKTFQGVVRTFCKSEVKESMLRSGVGVNLQLSLQLMSDAHASSMSEFSSSTSLLNLADQSQASNALVAWKELLYNIIANHYSDQKEALRVLGTLLLQENRVYAAHLVYILSLSPDVCSNKSNSLFELVGLSKHNLYPSHDDLFDVTQLTEVLELVFNVYSEKTPVFFTHLVPYRLYEAEVLAEAGEVSAARKYCELIGNYLNRVAKKSNNVDPGFVLRVRDLTQQILENSAGSEDISSSWLGRTVSRPRLDTVLSSLGSKFSKFVAGDPNFDVMRPATVGPGPFGKVASQKNLTVQTNTNNAAMESFYSDRPTSSGPSYQNRTPLTGQESMNMGVYSPYRRSTEIAENMSMDGNAYPYTPASQENPYTPRHSQEDNASVLSQQPLTFYSNVADNSYMPVSASQEPKMGMGTAFNMPTNEVHGVGAEMASPYQPLQPASAHLPNLQPTLAPINQNAYVPSNIAPAMGAMQSAPSAEAVAAPSESLNLNKDRSQQAKQAAAQNVADLVRQEEEKEKQKQKAKKNAESGKKGGAKGWFSKLLRRDESKDQPTVYKAKLGEKSHLHYDKELKRWVNDDGSDLSNQAAPPPPPPMALPKAGPPSAAPTSALPPAGPPAGATAISGNPGMPAPVPLTGKETAVPLSSMPNAPPSVASNAKLPPASNNRKVDPLEDILQAMPPPTTRKARGKTSKRYVDVMRNS

Target ORF information:

RefSeq Version	NM_001020416.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative multidomain vesicle coat component Sec16 (sec16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020416.2

ORF Insert Sequence:

ATGGATATTG AATCAGACCA CGAGCCTAAG GAGTCTTCTG TTGATCTTGA AGGACGGCAT 
GATTCTATAC GCAAGTCTTT TGAAGCTGAC GATATTGATG TGACAGATGA AAACACCATT 
TTTTCTACCT CTAGAAAAGA ACAAAGCACA ATTGAACCTA TGGATTCCTT TGTTGAAAAC 
AACATAGCCA ATAGCTCCTC TCAACAGTCA GTTTCAGAAA TTGACATTCA GTTGCCTTCC 
TCTGAGCCTA AAGAAGGTGT TAAAGAAGAA AGCAACTTAA TAGAGAGTAA TTCTTTGGAC 
AATGTGCAGG AAATTAATCC TCAAAATACC ATTGGCGACA AATCGGCTCA CACAGAAGAG 
GAGTCTTCAA ATGATAACTT GGTAAAAATT ACTGCTGGAA CCATTATTTC TGACATAATG 
GATGAGCCTG TTGTGGATGA AGACGATAAA CTCTCTGAAG AAGACTTGAG CAAGGAAGAC 
TTAGAAACGA CTCTTAAGGG TGACGAAAAC TATTCATCGT CGCAAAAAGA AATATATGAT 
AATGATGAGA CAATTCCTAA ACCACCATCT CCAGAAGTCG AAAAGATAGA AACTAGTACT 
TCAGTTGAGA ATGCATACAT TACGAACTCT CAATATAATG ACACTCTTGA TATGGAAGAT 
CAGACATCTG ATTTAATTAC AACTTTTGAG CATGAAAATG AAGACCATGA AGTCCACGAG 
GTTCCTTCCA TTCACGGAAT GGAAGGGACT TTTGAAACCG TAAATCTAAC TTCTGAAGCT 
GGGAATGAAA AAGAGTTTAA CAACGATTCT GTAGTATTGA ATACGACGCC AAAAGAAATG 
CAAATTTCAT CTTTAGATGC TGTTGACAAA CTTGAAGATA AGCCTGTTTC GAACGACAAT 
ATCAAAGCCA ATATAGAATC GTCGACTACA GTTGATAGGG CTTCATCTCA GCTTGAATCG 
GAAAATAACG AATCCACTTT TTTTGTACAT AACCAAAATT CCTCCGGGCA ACATCAAACT 
TTGGATTTCG TCGCAAGCAA ACCCGTTACC GATACCCCTG AGCTTAGTAA GGAAAATACG 
TTGGTGTCTT CTGAAAACCT AAATGATCCC AAACCAAATT TGCCGGAGAC TGAGGTTGAT 
TTTGGAGAGC CTTTGGAAGT TGAGGCTCCC AGTTTTGTGG TCCAGAATAA CAGTGCTGTG 
CAGCCAACGA CCCAGAAAAC TTCCGAAGAT TCAACCGATC TACACACCAA TGAGATCCCA 
AATGTTGCCC AACCTCCTTC TTCTTTTGAA AAGGAGAATA ACAAAGATAC TAGCAAGTTG 
GAAAATCCGG ATATTTCTTC ATCACCATTA AGTCCAACTG AAGATTTGTT TCCTAATGAC 
CCAGAAGAAG AAAATTTATT TTCGGCGGCT TTGGGCTTGA ATTCGAATAC TGGTTCGCAG 
CCATCAGAAA CCCAGAGTAA GCCATCAATC GATCCATCTG AATCAATAAC TGTAACCGAT 
AATCAAGATT CTCTTCTCTT TTCCCAACTC ACTAATAATG CTTTGCAAGC AGAAAATGCT 
ACGAAAGTAT CCGAAAACAC TATCAATGAT GAGGAGTTGA TAGATGATTC AGAATTTACT 
TCGCTTATGA GCAACTTTTT GGAAAGCAGT ACTGTTCAAA CAAATAAGTA TCTCCCAAAA 
TCATCAGCAT CTCCGCCAGC CAATGCTCCA ATTGTTTCTT CGGATGTTCA TAAAAATGAA 
AATGCTGGTA CAGCGCGTAG AGCTCCTCAA TCTGGAGCAT TCGTTGCATC AAAGGCGAAA 
TATTCTTCTC CTTATGATTT GCCCGAAGAA ATAGTACAAG TAGCACAGCA AAAAAGGTCT 
GTATCACAAA ATTACAACCG GCAGTACTCT TTTCAACCTC GTCCTGCTAC TCCATCCAAT 
CCCCCTCGTT CTTTACCTCC TCCTAGTGGT CAGGTGAATG CCCCAATGAG TCAAACCCCT 
AATCCAATTT CGTTTGCTTA CCAGCATGGT ACTCCTTTGG CGACACCTAC CATGCGCGCT 
AACTCTTTCA ATTCCTATCC AGCGTCTAGT GCTGAACCGA TCAGGAGACC GGCAACCACC 
ACCGTTGGAC ACACTCCTAA CTTGTACAGC CCAAAGACTA ATACGTACAA TTCTCGACAT 
ATGGCATATG AAATGACAAA ATCTCATATT AACGTTATTT CTCCGGGACC TTCTTTACAA 
GTTAATGCTC CATACACGCC GACTTCTGGC GAGCTTGGTA ATAAAGTTAG CAACCCTACC 
AAGGAATTCG TATCGACATC GTCTTATGCA CCTGCTGCCA ACACAAGAAA TGCTATTATT 
CGTGAACCCG GTATTCTATC ACCTCTTTCT CCTCGTGTAC AGCCTGTACT GTCTCGACGA 
GAAAGCATTA TTTCGATGGG TTCATCTGCC AGTAGTTATG TTCCTTTGGA AATAGCACCA 
AGACCGATGT CTTCGCTTGA GCATACTATG AATTCTGCGA TGTCTCCGGG TAATTTGCAG 
AGAACCGCAA ACCTGTACAA ACCAATGACG ACTCCCAATG CATATAATAT CAAAAATTCC 
AACCAGAGGG AAACAAAATA TCCTTATCAG CCGCAAGCTA TAAATTACTC TGAGGTTACC 
CAGCCTGGTA GTTCTTCGTT ACCGACAAGC GGAGAAGAAG CGAACATTAT CAGAAGTCCA 
GGATTTACAC CTTTAGCAGC ACAAAAGGAT GCAACCATAT ATACCCCTTC TCACGCACAA 
GCAACTTTAT ACGGTAATAT GGATAATAAC GACAGAGATA ATGAGGGTAT CCATGACATA 
TTACAATCAG ATATGGAACC TGTATTACCC CCTCATAACT CTGCTTACCA TGCTAATGCT 
CCCGTAAGTT CTCATTCAGA AGGACTGAAC AATAGACTTC CGATTTCACC CCTTCCACCC 
CAATTACACA AAACTGGCAC ACCTTCACAT CAACATGGAT TTGACACTGC TGAAACTACG 
GCGAAGCAAT ATGCACCATC TATTCCACCG AATTTTAATC CAAACGTAAG CATAGATACA 
ATGACTGAAG GGGTTGCGCT CCCTTCGGCT ACTCTTGATT CTGATAAAAG CTCGTTGCAT 
AAACGTAGCG CTGAATTATC ACGAAATAAT TCTCCCCGCC CTGATTTCTT ACCATTACCA 
AATCAGCCAC TTTTGCATTC TAATTTGCAT TCGCCTGTCT CGCCAGTTGT TGATAGTAAT 
GAAGACTCTC GACTTAAATT TTTAAGTACG CAAAGACCTG CATTTTCGTT TGGACCTTGT 
GGTACAATCG TTATGGCGTT TTCCACTCCC AGTGGTTTAT ACACAACTAG TGGCAAAGGT 
ACCAAATTCA TTGCAGGACC CATTAAAATA GAAAAATTGG GTGATGTGCT AACGGATGAG 
TATCGTCATC TAAAGGAGTT TAAAGGACCA TACCTTGCCT CTAATGGAAA GGTAGATAAA 
CATGGAAAAG CCGAAGCTAT TGAATGGCTT TCCAAATATA TTGACCGTCT AAACCAATCG 
CTAGAATACG ACGATAAAAA CATTACGCTT AAGGACAAGT TACTTTTACT ACAGTGTTTG 
AAGATGCTAC TGGAAGTGAG TGATCGTAAG CTAATAGTTG AAAAGCTTCG TCCTATCTTA 
TTACCTTCCT TTGAAATTCC TGAACCTTGC AATACAGCTA CTTCAGTTCA AGAGCTTATT 
AATCCTGAAA TTAATCAGGA TGATTCTCCA ATTGTCGCAT CTCGTTATTG CACAACTTCT 
TTCCTTCACC GATTTTATGA ATATTTATTG TCTGGCAACA AAGATGAAGC GCTTACTTAT 
GCTTTGCAGC AGAAGCAATG GCCATATGCG ATTATTGTTG CGCATTCTAT AGATGCAAAA 
ACATTTCAGG GAGTAGTACG AACGTTTTGT AAATCAGAGG TCAAAGAATC TATGCTTAGA 
TCTGGTGTTG GAGTAAATTT ACAATTATCT TTGCAATTAA TGTCTGATGC CCATGCGTCT 
TCAATGTCAG AGTTTTCGTC TTCTACGTCA CTATTGAATT TGGCGGATCA ATCGCAGGCA 
AGTAATGCTT TGGTAGCCTG GAAGGAGTTG CTGTACAATA TTATTGCAAA TCATTACTCT 
GACCAAAAAG AAGCATTGAG GGTTTTAGGG ACATTACTAC TCCAGGAAAA CCGTGTTTAT 
GCGGCACATC TGGTTTACAT CTTAAGTTTA TCACCGGATG TTTGCTCGAA CAAATCGAAT 
TCTCTTTTTG AACTTGTTGG TTTAAGTAAA CATAACTTAT ATCCTTCGCA TGATGATTTA 
TTTGACGTTA CACAATTGAC CGAAGTATTG GAGTTGGTAT TCAACGTCTA TTCTGAAAAG 
ACACCTGTTT TTTTTACTCA CTTAGTACCA TATCGCTTGT ATGAAGCTGA AGTTTTGGCT 
GAAGCTGGTG AGGTATCTGC TGCAAGAAAA TACTGTGAAC TAATCGGAAA CTACCTAAAT 
CGTGTTGCAA AGAAGTCAAA CAATGTTGAT CCAGGTTTCG TTCTTCGTGT TCGCGATTTG 
ACCCAGCAAA TATTGGAGAA TAGTGCAGGC AGTGAAGATA TCTCTTCGTC TTGGCTTGGT 
AGGACAGTGT CTAGACCTAG ACTGGATACA GTTTTATCGT CGTTGGGTAG CAAATTTAGT 
AAATTCGTAG CTGGAGATCC CAACTTTGAT GTTATGCGTC CAGCTACGGT TGGGCCGGGA 
CCATTTGGAA AGGTTGCCAG TCAAAAGAAT TTAACAGTTC AAACAAATAC TAACAATGCT 
GCCATGGAAT CATTTTATTC CGACAGACCT ACGTCTTCTG GACCTTCCTA CCAAAACCGA 
ACGCCTTTAA CAGGCCAGGA GTCAATGAAT ATGGGAGTTT ATTCACCTTA TCGTCGTAGC 
ACAGAAATTG CTGAGAATAT GAGTATGGAT GGCAATGCCT ATCCTTATAC TCCAGCTTCT 
CAAGAGAATC CCTATACCCC GCGTCATTCT CAAGAAGACA ATGCATCTGT ATTGTCACAA 
CAACCCTTAA CATTTTATTC AAATGTCGCA GACAATTCAT ATATGCCTGT GTCTGCATCA 
CAAGAACCGA AGATGGGTAT GGGAACTGCA TTTAATATGC CGACAAATGA GGTTCATGGT 
GTTGGAGCAG AGATGGCATC GCCTTATCAA CCACTTCAAC CGGCATCAGC TCATCTACCA 
AACTTACAAC CTACCCTGGC ACCCATCAAT CAAAATGCCT ATGTACCTAG TAATATTGCT 
CCGGCGATGG GGGCAATGCA AAGTGCACCG AGTGCAGAAG CAGTGGCCGC ACCATCTGAG 
AGTTTGAATC TCAATAAAGA TCGAAGTCAG CAAGCCAAAC AAGCAGCAGC ACAAAACGTG 
GCAGACTTGG TTCGTCAGGA AGAAGAGAAG GAAAAGCAAA AGCAAAAAGC AAAGAAGAAT 
GCAGAATCTG GTAAAAAAGG AGGAGCAAAG GGATGGTTCT CAAAATTGTT AAGACGGGAT 
GAGTCAAAGG ATCAACCCAC CGTGTATAAA GCGAAATTAG GGGAGAAATC CCATTTGCAT 
TATGATAAAG AATTGAAACG ATGGGTCAAT GATGACGGCA GTGATTTGAG TAATCAAGCA 
GCACCGCCTC CACCACCTCC CATGGCGCTT CCCAAAGCCG GTCCTCCTTC CGCAGCACCT 
ACAAGCGCTC TACCTCCTGC TGGTCCACCT GCAGGTGCTA CAGCAATTTC CGGGAATCCA 
GGAATGCCGG CGCCTGTACC GCTTACTGGT AAGGAGACCG CTGTTCCTCT ATCCTCTATG 
CCGAATGCGC CTCCCAGCGT TGCTAGCAAT GCCAAGTTAC CACCGGCTAG CAACAATCGA 
AAAGTTGATC CATTAGAAGA TATTTTACAA GCGATGCCAC CTCCGACAAC ACGTAAGGCT 
AGAGGGAAAA CAAGTAAAAG ATATGTGGAT GTCATGAGAA ATAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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sec16 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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