lrs1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following lrs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lrs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OSc103962 NM_001020311.2
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Schizosaccharomyces pombe putative leucine--tRNA (Leu) ligase Lrs1 (lrs1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc103962
    Clone ID Related Accession (Same CDS sequence) NM_001020311.2
    Accession Version NM_001020311.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3336bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative leucine--tRNA (Leu) ligase Lrs1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001020311.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCTACGA CTGAACCTTC CGTCGAGCAA CTAGAGACGA AGACTGCAAA GTTGAAGTTG 
    GAAAACACGA CGAAGCGTGA CACTCTAATT GAGTTGGAGA AAAAATATCA ACAAAAATGG
    CAAGAAGAAA AGGCATTTGA AGTCGATGCT CCTTTGGAAG ATGTTCCGAT CGATGAACTT
    CGTAAGAAAT ATCCTAAATT TTTTGGTAAC ATGCCTTATC CTTATATGAA TGGTGCATTG
    CATCTTGGCC ATGCCTTCAC TTTGTCTAAG GTCGAGTTTA CTACAGCTTT TGAACGCTTG
    AATGGAAAGC GAGTGTTGTT TCCCATGGGC TTTCACTGCA CCGGTATGCC TATTTGCGCT
    AGTGCTGACC GTTTGTCACG TGAAATAGAG ATGTTTGGAC CCAGCTTTGA CGTTCCTGAA
    GAAAAGGAAG AAGAGGTAGA GGTCGAAGTT AAGACTCCTA ATGCTCGCGA AGATGTCACT
    AAGCACAGCG GTAAAAAGAG TAAGGCTGCG GCTAAAACAG CTGCTGTAAA GTACCAATTT
    CAAATTATGG AATCTCTTGG TGTTCCCCGG ACTGAAATTC ACAAGTTTGC TGATGCAAAG
    TACTGGCTCT CATATTTCCC TCCCTTGTGC CAAAGAGATT GTACTGAATT TGGTCTCGGT
    ATTGACTGGC GTCGTTCCTT TATCACTACT GATGTCAATC CTTACTATGA CTCCTTTGTA
    CGTTGGCAAG TAAATCATTT GCACGATTCT GGGAAAATTA AGTTTGGTGA GCGCTATACT
    GTTTATTCCA TCAAGGACGG TCAACCCTGT ATGGATCACG ACCGTAAAAG TGGTGAAGGT
    GTTGGTCCTC AGGAGTACAC TGGTATCAAA ATGGAAGTTT TGGAGTTCCC CGAGGCTGCT
    CGTAAAGCTC TCCAATCCAT TGATCTTTCC AATAAGAAGG TCTGTATGAT TGCAGCAACG
    CTCCGTCCAG AAACCATGTA TGGCCAAACA AATTGCTATG TTGGCCCCAA CATCACCTAT
    GGCATTTACG AAAGTAATGT TCCCAATGAG CTTTTCATTT GCACTCGTCG TGCTGCTAAC
    AACATGGCAT ACCAAAAGCT TTCAAAGGAA CGTGGTGTAG TCAGCGAACT TGGAACCATT
    AAGGGACAAG ACCTTATTGG CGCCCTTGTT AATGCACCTC TGTCCGTGCA CAAACAAGTT
    TATGTTTTAC CTATGGAAAC AGTTTTGGCT ACTAAGGGTA CTGGCGTGGT CACCTCTGTG
    CCTAGCGACT CTCCTGATGA CTTTGCTACT TTAACTGAGC TTCGCAAAAA GGCTGAATTT
    TATCACTTGA ATCCCGAGTG GATGAAGTAC GAAGCTGTCC CTATTATTCG CACTCCTTCT
    TATGGTGACA TGTGTGCTGA GTTTTTGTGC AAAAAACTTA AGATTCAAAG TCCTAAGGAT
    GTTAAACAAT TGGCTCAAGC TAAGGAGCTT GCTTATAAAG AGTGTTTCTA CCAAGGTACG
    ATGATTATCG GTAAATATTC CGGTGAGAAG GTCGAGACAG CCAAGCCCAA GGTTCGTAAG
    GAGTTGATTG ATCAAGGCCT TGCTTTTGTT TATAATGAAC CCGAAGGACA AGTTATTAGT
    CGCAGTGGTG ATGATTGTAT TGTTGCCTTG TGCGACCAAT GGTTTTTGGA TTATGGCGAG
    GCTTCTTGGA AGGCCGTAAC CGAGAAGGCA TTGGATCGTT TGAACACTTT CTCACCCGAG
    GTCCGTAATG GTTTTTTGAA AACCTTGGAT TGGCTTAGCC AATGGGCTTG TGCTCGTTCT
    TATGGTTTAG GTACTCGCTT GCCATGGGAT CCTCAGTTCC TTGTTGAAAG TTTGACCGAC
    TCGACCATCT ACATGGCCTA TTATACAATT TGCCATCTTT TGCATAGCGA TGTATATGGT
    AAGGTACCGG GTGCTTTGAA CATTAAGCCT GAGCAAATGA CTCCTGAAGT ATGGGATCAT
    GTTTTCCGCC AAGCACCAAA GCCTAAAAAC ACCAGCATAT CCGATGAAGC ATTGGCTCGT
    CTTTGCCGTG AGTTCCAATA CTTTTATCCT TTTGATATTC GTGCTTCCGG TAAAGATTTG
    GTTCCTAATC ATCTTACCTT CTGTTTGTAC ACCCATACCG CCATTTTTGA TGAGAAGTAT
    TGGCCCAAAG GTATACGCGC CAATGGCCAT CTTCTCATGA ATGGTGAAAA GATGTCTAAA
    TCCACTGGTA ACTTTATGAC TCTTCATGAG GCCACCAAGA AATTCGGTGC AGACGCAACT
    CGTTTAGCTC TTGCTGATGC TGGTGATACT GTTGATGATG CAAACTTTGA AGAGGCTTTG
    GCCAATTCTG CTATTCTTCG TCTGTATACC CAGGAAGCCT GGTGTAAGGA GATGATGGAA
    AACCTTGACA ATTTACGCAC AGGCCCTTAC AACTTCCATG ATAAAGTTTT TGAAAACGAG
    ATCAATCAAC TCATCGAATC ATCTAGAGAA GCTTTTTCTG CTACTCTTTT CAAGGCTGCC
    TTGAAGAGTT GCTTTTACGA TCTTCAAAAT GCTCGTGATT GGTATCGTGA GGTAACCGCT
    GATCGTAAAA TGCATCGCGA TCTTGTTTGT CGTTGGATAG AAACTCAAGT GCTTCTGCTG
    GCTACATTTG CACCTCACTG GTCAGAACAC ATCTGGCTTA CAACTCTCAA GAAACCTCAG
    AGTATCCATG TTTCTGGTCG TTTCCCCCAA GTTTCCAGCC CTGTAAACAC GGCTTTATCT
    AACTCCTTGT TATACATCCG TACACTGAGC CGTGTAATTC GTGAAGCCGA GGCAGCTCAA
    CTCAAGCGTC AAAAGAAAGG TAAAGGTATG CTTTTCGATC CCTCCAAGCC TAAGCGTCTT
    ACTGTGTTCG TCGCTGAAAA GTTCCCCGAG TGGCAAGCTC AGTACGTTGC TCTTCTTCAA
    AAATACTATA ATGAGTCTGA GAATAAGTTT GATGATAAAG CAATCATTTC TAGTGTTGAT
    AAAAAGGAAA TGAAGCGCGC AATGCCCTTC ATTCAGCAAT TCAAACAATC TGTCATTAAC
    CGTGGAGAGC ATGTGTCAGC CAATTCCATA TTTAGTCGTG AACTCGGATT CAATGAGCTT
    GAAGTTTTGC GTGAGGTTAA GCCTTACCTT GTTCGAAACG TTGGTATTCA AGAGCTTCGC
    ATTGTCCTTC TTCAAAAACC TGCAGATAAA TCTTCAGCTG CTATTGGTTT GGTTGAAAGT
    GGATCGGATG CCGGTGCTAC TGTAGAGATT GCACCTAATT TTGCAAATAC CGTACCCGGT
    CAGCCTACAT TTTTGTTTGA AAATGTCTCT GCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_594882.1
    CDS165..3500
    Translation

    Target ORF information:

    RefSeq Version NM_001020311.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative leucine--tRNA (Leu) ligase Lrs1 (lrs1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001020311.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCTACGA CTGAACCTTC CGTCGAGCAA CTAGAGACGA AGACTGCAAA GTTGAAGTTG 
    GAAAACACGA CGAAGCGTGA CACTCTAATT GAGTTGGAGA AAAAATATCA ACAAAAATGG
    CAAGAAGAAA AGGCATTTGA AGTCGATGCT CCTTTGGAAG ATGTTCCGAT CGATGAACTT
    CGTAAGAAAT ATCCTAAATT TTTTGGTAAC ATGCCTTATC CTTATATGAA TGGTGCATTG
    CATCTTGGCC ATGCCTTCAC TTTGTCTAAG GTCGAGTTTA CTACAGCTTT TGAACGCTTG
    AATGGAAAGC GAGTGTTGTT TCCCATGGGC TTTCACTGCA CCGGTATGCC TATTTGCGCT
    AGTGCTGACC GTTTGTCACG TGAAATAGAG ATGTTTGGAC CCAGCTTTGA CGTTCCTGAA
    GAAAAGGAAG AAGAGGTAGA GGTCGAAGTT AAGACTCCTA ATGCTCGCGA AGATGTCACT
    AAGCACAGCG GTAAAAAGAG TAAGGCTGCG GCTAAAACAG CTGCTGTAAA GTACCAATTT
    CAAATTATGG AATCTCTTGG TGTTCCCCGG ACTGAAATTC ACAAGTTTGC TGATGCAAAG
    TACTGGCTCT CATATTTCCC TCCCTTGTGC CAAAGAGATT GTACTGAATT TGGTCTCGGT
    ATTGACTGGC GTCGTTCCTT TATCACTACT GATGTCAATC CTTACTATGA CTCCTTTGTA
    CGTTGGCAAG TAAATCATTT GCACGATTCT GGGAAAATTA AGTTTGGTGA GCGCTATACT
    GTTTATTCCA TCAAGGACGG TCAACCCTGT ATGGATCACG ACCGTAAAAG TGGTGAAGGT
    GTTGGTCCTC AGGAGTACAC TGGTATCAAA ATGGAAGTTT TGGAGTTCCC CGAGGCTGCT
    CGTAAAGCTC TCCAATCCAT TGATCTTTCC AATAAGAAGG TCTGTATGAT TGCAGCAACG
    CTCCGTCCAG AAACCATGTA TGGCCAAACA AATTGCTATG TTGGCCCCAA CATCACCTAT
    GGCATTTACG AAAGTAATGT TCCCAATGAG CTTTTCATTT GCACTCGTCG TGCTGCTAAC
    AACATGGCAT ACCAAAAGCT TTCAAAGGAA CGTGGTGTAG TCAGCGAACT TGGAACCATT
    AAGGGACAAG ACCTTATTGG CGCCCTTGTT AATGCACCTC TGTCCGTGCA CAAACAAGTT
    TATGTTTTAC CTATGGAAAC AGTTTTGGCT ACTAAGGGTA CTGGCGTGGT CACCTCTGTG
    CCTAGCGACT CTCCTGATGA CTTTGCTACT TTAACTGAGC TTCGCAAAAA GGCTGAATTT
    TATCACTTGA ATCCCGAGTG GATGAAGTAC GAAGCTGTCC CTATTATTCG CACTCCTTCT
    TATGGTGACA TGTGTGCTGA GTTTTTGTGC AAAAAACTTA AGATTCAAAG TCCTAAGGAT
    GTTAAACAAT TGGCTCAAGC TAAGGAGCTT GCTTATAAAG AGTGTTTCTA CCAAGGTACG
    ATGATTATCG GTAAATATTC CGGTGAGAAG GTCGAGACAG CCAAGCCCAA GGTTCGTAAG
    GAGTTGATTG ATCAAGGCCT TGCTTTTGTT TATAATGAAC CCGAAGGACA AGTTATTAGT
    CGCAGTGGTG ATGATTGTAT TGTTGCCTTG TGCGACCAAT GGTTTTTGGA TTATGGCGAG
    GCTTCTTGGA AGGCCGTAAC CGAGAAGGCA TTGGATCGTT TGAACACTTT CTCACCCGAG
    GTCCGTAATG GTTTTTTGAA AACCTTGGAT TGGCTTAGCC AATGGGCTTG TGCTCGTTCT
    TATGGTTTAG GTACTCGCTT GCCATGGGAT CCTCAGTTCC TTGTTGAAAG TTTGACCGAC
    TCGACCATCT ACATGGCCTA TTATACAATT TGCCATCTTT TGCATAGCGA TGTATATGGT
    AAGGTACCGG GTGCTTTGAA CATTAAGCCT GAGCAAATGA CTCCTGAAGT ATGGGATCAT
    GTTTTCCGCC AAGCACCAAA GCCTAAAAAC ACCAGCATAT CCGATGAAGC ATTGGCTCGT
    CTTTGCCGTG AGTTCCAATA CTTTTATCCT TTTGATATTC GTGCTTCCGG TAAAGATTTG
    GTTCCTAATC ATCTTACCTT CTGTTTGTAC ACCCATACCG CCATTTTTGA TGAGAAGTAT
    TGGCCCAAAG GTATACGCGC CAATGGCCAT CTTCTCATGA ATGGTGAAAA GATGTCTAAA
    TCCACTGGTA ACTTTATGAC TCTTCATGAG GCCACCAAGA AATTCGGTGC AGACGCAACT
    CGTTTAGCTC TTGCTGATGC TGGTGATACT GTTGATGATG CAAACTTTGA AGAGGCTTTG
    GCCAATTCTG CTATTCTTCG TCTGTATACC CAGGAAGCCT GGTGTAAGGA GATGATGGAA
    AACCTTGACA ATTTACGCAC AGGCCCTTAC AACTTCCATG ATAAAGTTTT TGAAAACGAG
    ATCAATCAAC TCATCGAATC ATCTAGAGAA GCTTTTTCTG CTACTCTTTT CAAGGCTGCC
    TTGAAGAGTT GCTTTTACGA TCTTCAAAAT GCTCGTGATT GGTATCGTGA GGTAACCGCT
    GATCGTAAAA TGCATCGCGA TCTTGTTTGT CGTTGGATAG AAACTCAAGT GCTTCTGCTG
    GCTACATTTG CACCTCACTG GTCAGAACAC ATCTGGCTTA CAACTCTCAA GAAACCTCAG
    AGTATCCATG TTTCTGGTCG TTTCCCCCAA GTTTCCAGCC CTGTAAACAC GGCTTTATCT
    AACTCCTTGT TATACATCCG TACACTGAGC CGTGTAATTC GTGAAGCCGA GGCAGCTCAA
    CTCAAGCGTC AAAAGAAAGG TAAAGGTATG CTTTTCGATC CCTCCAAGCC TAAGCGTCTT
    ACTGTGTTCG TCGCTGAAAA GTTCCCCGAG TGGCAAGCTC AGTACGTTGC TCTTCTTCAA
    AAATACTATA ATGAGTCTGA GAATAAGTTT GATGATAAAG CAATCATTTC TAGTGTTGAT
    AAAAAGGAAA TGAAGCGCGC AATGCCCTTC ATTCAGCAAT TCAAACAATC TGTCATTAAC
    CGTGGAGAGC ATGTGTCAGC CAATTCCATA TTTAGTCGTG AACTCGGATT CAATGAGCTT
    GAAGTTTTGC GTGAGGTTAA GCCTTACCTT GTTCGAAACG TTGGTATTCA AGAGCTTCGC
    ATTGTCCTTC TTCAAAAACC TGCAGATAAA TCTTCAGCTG CTATTGGTTT GGTTGAAAGT
    GGATCGGATG CCGGTGCTAC TGTAGAGATT GCACCTAATT TTGCAAATAC CGTACCCGGT
    CAGCCTACAT TTTTGTTTGA AAATGTCTCT GCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L