tra2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	tra2
Entrez Gene ID	2541659
Full Name	phosphatidylinositol kinase-related protein Tra2
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018262.2	NP_592862.1	phosphatidylinositol kinase-like protein Tra2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tra2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tra2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc103709	NM_001018262.2 Latest version!	Schizosaccharomyces pombe phosphatidylinositol kinase-like protein Tra2 (tra2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tra2 ( NM_001018262.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_592862.1 Schizosaccharomyces pombe phosphatidylinositol kinase-like protein Tra2 (tra2), mRNA.

Price & Availability↑

CloneID	OSc103709
Clone ID Related Accession (Same CDS sequence)	NM_001018262.2
Accession Version	NM_001018262.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	phosphatidylinositol kinase-like protein Tra2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018262.1.

ATGGAAGACG GTTTTGAAAA GTCGCTCAGC AGCTCTATAG AGCTACTGAA AGCTGAAAGT 
ACAGAAATAG AAGAAAAAAT CAAACTAACT AAAATTTTAA TTACTAAGGT TTCTAGCATA 
TCTTCAGACG CCTCGCAGCT GTATGCAGAA TTAATTCCAC TACTCTTGGA TTTTTTACGA 
AATACAGAGG TGAATCTTTA TAGAAATTCT TCTGTCAATG AACTCAAGAT CGAAGCATTG 
ACTTTAATAC AAAGTTGCGC TCACCGTGAT GAATTTAAAC AATATGCGCA GTCTTGTGTG 
CTTTCGTTCA TTAGCTTGAT TAAAGCAGAT AATGAAGAAG TCGCTGTTTT TTGCTTAAAA 
GTTATTATGG ATATATTCAA AACTTTTAAG TTTTGTATAG AAAGCACTGC ACAACCCTTT 
TTTGACTTGG TACTTGAGCT TTCTACAAAC CTTCCTTATT TAATTCCCTC AATATTTGTC 
GAGAATCCAA AATCAAATGA AGAGGAAAAT ACAACCCTGG CTTTTGGTAG TTATCTGTCT 
ACGGAAACTT CAATCATCCA ACAGCGTGTC AATTCTTTAG CTATTTCTAC TCAACCTTTG 
GAACTGGCTT CTCAAAGTTT CCGCGTGTAT GTAGAGTGTC CGGTCATTAT AGTTTTGATT 
CTTCAAGCAT ATCGTCAAGC TGCTTTCCCT GGTGTTCAAG CCATAATTCC CTGTTTTCTT 
AAGATGGTAC AAATTGATGT TCCAATAGAC ATCGCTTCAT ATGCAATGAT TGAAAAAGAT 
TCAAGCATTG ACTTTATTGA GTTTATAAGG AATAAATATC AGTACCGTAA TTTTTTTATG 
GCTCAAGTAA AGACTTTATC TTTCTTGGCT TATATTTTAC GAACTCATCC CAATACACTT 
TCTGAGAAGG ATATAATACC GGACATTGTA ATAAAGCTTT TGAGGCGATG CCCTTTTGAT 
ATGTGTTTTG CCCGTAAAGA ACTGCTGGTT GCTACGAGAC ATATTCTGTC AACAAACTTA 
AAATCTCTTT TTGTCAAAAA GCTGGATTTT TTGTTAGATT TAAATATATT ATTAGGAAAT 
GGAGTTGGTA CTCAAAAGCT GTTACGTCCC TTGGCATTTA GTACATTAGC TGACTTACTT 
CATCATGTAC GTGACGAACT TAACGAAACG CAAATAAGAA AATCTATAAT GATTTATTCG 
ACCAATATGC ATGATTTGAC TCTTAGCATT GGGTTACAGA CTATGGGTGC CCGGCTCATT 
TTAAACATGG TTGACAGAAT GATTAGTCTT CCTAGCATCC CCGATGCTAT ATTTCTTCTT 
TTATCTATTT TTGATAGCTT TGTAAATAAG TTTTCGGAAC TTAATGATAG TTTGGATCAG 
TTTTTTAAGA AAAAGTATGA AGAAGAAATT AAAGAGACAA AGTCACCGAC GCGTTCTTCT 
CCTAGGGATT TATCTTCATT TAGCACTTCC GTCAATGATG GATCATTTCT TTTTAAAAAC 
TTGATGTTTG GCTTACGGGC TCTTATGTAT GGGCTTCGTA CATGCAAGTC TCGATGCATA 
GAAATTGGAG GTGAACAATT TTCGGGATTC CTTACGAATA TCAAGCCTTT CGAAGCTGTT 
ACGTTTCAGA AGCTTTTTTT TGAGGTTGGC AAAGGATTCT CATATTTTCG ACCTGAGCAA 
GTCTATTTAG AAACTTTTTT CTGCTGCGAA GAAGAAAGTT TAGATCGCCC TGCTATATCT 
ACGCTTCCTC GTAACAAAGA CGAGAAAGAT TGTCTTGAAG TATTTGCTAC CATTTTTATA 
CATCTCGAGC CATCTATATT TCTTAAAGTT TTTGAAACAA ACCTTCCTAC TTTTTTTGAT 
CAACTTAAAA AAAATCTCAC ATTATTTCAC ATTCCACAGT TTTTGCTTTC AAATGAGAGC 
ACATCATCAA AATTTTTAAA TATTTTACTT CGATTTCTGC TTTCCAGAAT TGAGGAACTC 
GGATCTTCTG ACATCAGGCA TGGTTCTGTC TTGTTGCGTT TGTTTCGTCT TTCTTTCGTT 
ACAGTCTCAA TGTTTGCAAC AGAGAATGAG CCAGTTCTTC GGCCTTATGT ATCTGAAATT 
ATTGTGAAAT GTATGAAGTT GGCTCCCAAT TCTGCTAATT CATTGAACTA CTATTATCTA 
TTAAGAGCGT TGTTCCGAGG AATTGGTGGG GGAAGATTTG AAAGCCTTTA TAAAGAAGTA 
ATGCCTTTGT TGCACGCTCT ATTAGAGGCA TTTAATTCAT TGCTTATATC TGCCCGTACT 
CCGAAGGAGA AAGACCTTTT TACCGAACTT TGTCTTACTA TTCCTGTAAG GCTTAGTCTT 
TTATTACCAT ACATGAGTTA TCTAATGAGA CCTCTCGTCA TGTCTTTAAA AAGCAGTCAA 
GAATTAGTTA GTCAGGGATT ACGAACCTTT GAATTATGCT TGGATAATCT CACTCCTGAT 
TTTTTGGATC CGATAATGGC TCCTTACATA GAAGATCTAA TGAATGCTTT ATGGAGTCAT 
CTACAACCTC TTCCTTATAA TTATAATCAC AGCCATACTG CCTTGAAAAT ACTTGGTAAA 
CTTGGTGGAA GAAATAGGAA GCTTCTTGAT AGAGTCCAGA GTTTAAAGAA TTCGCCGGAA 
CCGAATAACG ATTTTACTTT ACTTCTTTCG ATAAAAGGGG TTAAACAACC ACAACTTTTA 
CATTACACCC AATATGTTGA TGAGGCTGTG AATTTGCTTT CTAGCCCTTC TAGTGATCTG 
GAGGTTAAGC AACAAGCGTT CACTTATGTT TGCAATATTT CAAAGCTCTA TGTTTATAAG 
AGTGACGCTA CTAACAGCTT AGCCTCATCC ATTCGATGTA CTGCAGACAA GATTTCTAAG 
AGTAATTTTG ATTTTCGACG ACCATATTCT GTAATACCGA GCCGAATGAC TGGAAGGAGT 
TCATTCACCC AACTGAGTGA TGATTCAGAT GAAACCATAA TTTTGGCCTC GGCTACATAT 
GGACTTTTTT TTGCTACGAC GGTTGATGAA CTCCGCGAAG AAGCTTACTT CTGGCTTGAA 
AAACTGGCGG TCAACGTAAT TGTACACGAT ATTTTTTATG CTTTTGACGT TATTCAAGGA 
AATCATTCAA AATTTACGAC TAATTTGCAA AAGGAGGTTA TTATTAGTCC TCATTATTTT 
GCTCATTGTC TATCAGAAGT CGTTTGTAAT GACAATAGCA AGATGGGTGA TGCTGTGAAA 
CATGTACTTA AATTTATGTT TAGTTTTTTA GAATCGATTT TTGAAAATCC AGAAAGGGCT 
TTTATCCTTC CAATTTTTGA GGTCTTATTA TCTGATTTCC GGCACAAATG TTATGACCCT 
CATTGGTATA AAAAATACGG AGGCTGTTTT GGATTGGAAT GTTTGATAGA GCAAGATCAT 
TCTTCTAAAT GGCTTTTTGA CAGACAAGTT GATATTTTAA CTGCGCTATT TTTTACATTG 
AAAGATACTA CCTCAGAAGT CCCTACTGTC TGTAAGGATC ACGTGATGGA CGTTTTAAAG 
CAGTTATTTC GAAAAATATA TGCTTCCAAA GATACTGAAA TTGCTCCCGG AATTTTAGGG 
CATCTAGTAT TGGAATTGTC AAATCATAAT TCTGTGGTCC GTTCTTCTAC CCAAAAATTG 
CTTTCTTTAT TGTCGGAACT TTCAAATACT CCGATTGTTA AACTAGTTTC ACCTTTCAAA 
GAAAGACTTC TCTCACCAAT ATTTGCAAAA CCTCTTCGAG CATTACCTTT CCATATACAA 
ATTGGACATA TCGATGCTGT AAATTACTGC ATAAGTCTTG GAAGTGAATT GATTACTTTT 
TCCGACGAAT TAATTCGTCT TATTCATGAA ACCACTGCTT TAGCAGAAGC GGACGATGAT 
GCTCTTATTG GAATTGGTAA AACTTCTCAC TTCAAAAACG CTGCACTTCT TATACGTTTG 
AGAGTAGTAT GTGTAGAATT ACTCAGTACT TGCATTTTGA AGATTGATTT TAATAACCCT 
CAACATGCAC ACTTACGTGA AGGAATTATT GTTGTTTTTT TTAAATCTTT GTATGCAAAG 
TCCAAGGAGC TTATTGAAGC GGCATCATTA GGTCTCAAGA ACGCTTTGCA AGAGGACCAA 
AAGTTATCAA AAGAATTGTT ACAAACAACT TTGCGTCCAA TACTGTATAA TATATCTGAC 
TATCGAAGGT TAAGCGTTGC AGGACTTGAA GGTTTGGGAA GGCTGCTACG CCTCCTCACA 
AATTACTTTA AGGTCGAGAT AGGAAGAAAA TTACTAGATC ATTTAAAGGC TTTACTTGAA 
AATGTTGATT TCCAAAAGGT GTCTAGTCTT CCTTTGTTTT GTCAAACTGA AATGAAAATT 
GTTCGAGCTC TTATTGATCT TTTTCATTTA TTACCAAATA ACGCAAATAG ATTTATGGAT 
GAATTGTTAA TTTGTGTTGT AGAATTTAGT CTGAGACTAC AAAGAACCTT TCCTAATTAT 
TTCAGTGAAC CTTTGCTAAA ATATGTCAAT AGATATCCCG AAGATGCTTG GAAATTTTTT 
ATGGTTAGAT ACGATGAGGC CGCTTTTACG TCTGTTTTTG TTGAGTTACT TCGACTTAAA 
GATTCAGACC CCCTACTTTC AGTTGTGAAG GATAATTGGT TATTTTTTCA GACCATTTTA 
ACTAACGAAA TCAATACTGT AACTGCTAAC CGCTATTCAT TTGCTTTGGA CTCTGCCATT 
GTAATACTAC AACGTGATCC TTCTTTCTTC AAAGATAAAA ATGACTTTTT TAGAGGCTCG 
ATGGATGCCG TGCTAGCAAT AAGCCATCTG GTTGAAAATG AGTCCATTTT GGAATCCATG 
GTTTTTTGGA ACGACCTGTT GGTTCGTACC TCAACATTTT TGTTGGAAGT GTATGATTTA 
TGCATATATA ATTATGACGA TGGGCTCAAG TTACTGTCAT GCTTTCACAT GTATAAAAAC 
TCATTGGCCA AGAACCTTGT AAGTGACCTT ACGGCTCATT TGGTAAAAAA AATTGAAGAA 
CCTGATTTAG AGAACAATGT TAAATTGATT TTAAACCTTA TTTTATCAAA AGACTACGGG 
TTTTTATTGA AGGAAAACTT GGCTGGCATT CTTCTCACGT ATCTCAATCA GAATGTGTCT 
TCTCTTGAAA AATGTAATCA AATATTTTCT ATATTTTACG AAGTTTTTTT CCAACATCCT 
TCCACTAATG TTTATGCTAA TGATGAGGGA ATAAAAATAG GCGCTTTACA AATAATTTCT 
TTTTTTTTAA AAAATGTCCC AGAAATTACT GTACAGCATC AGACTGAAAT GCTTAAGATG 
TGCAGTCTCT TTGGAAATTC AGAGGACGTG ATGATTAAAC AATTATCGAT TTATGTCATG 
TCTTTATTCA TTTTACGATC ACAATTTCCG TACGAGCTAG TAAACGTTGT GTATATGGCT 
CTTTTGAAAA GTTCCCCTAT TGAAGTTCGA CATTTGGTAA AATCTTCTTT TGACAACATA 
TTTTCGTACA TATTTTCTGA GGAGCCAGAA AGCAAGAAAT CACCTATCTG GTTTGAATTG 
CCTTTACAAG TTATTAGCAG CCAAAGTCAA AATATTTCAC AGCTGCTCAA TGTTTACGAT 
TTTATTAGTA GTCACTCCGA TATTTTTATC GAACACAGGG GAAGGTATGT TCCGATTTTA 
ATTGATTCTT TATACAAATT TGGAGCCATT CCGAACCCTA ATCCTGAGAT TCGCGCTCTA 
TCTTTAGGCT TAATTAAGGT TTTACTAGAA TGGAATGATC TCCAACTTAA AGTTGACCAG 
AAGGAAATTT TTTCAAATAA CCAGAAGAGA GCTATATTGA GTTACCTATT TCGTTTTGTG 
TGCCTTTTTT CTGAACCCTT CACAGAAGGT TTGTGTTCCG AAGCTATTTC TTTACTAGAG 
CGTTTGCTTT CATCCGGTAC ATGGGCAAGT TTAGGGATGA AACTATCTTT TTTTACGAAG 
AGTATTACTC ATTTTGATGC TACTGATGCC AATTCAGTGA TGTTTGCCAA TTCGCTAAGA 
ACCCTGTCAA TCGTGGTGGG GCACTCAGAT TCTGCGTGGA TAGAAGAAAA CCTTTCCGAT 
TTGAAGTTTT TATTAGAAAA ATCTTTAGAA AATGAATCAG TTGGTGTACA GAGTGCAATT 
GGAAATTTTG TTTCAACTAT ACTAACGCTC TCCAACACTC ATCCTTCAAT TGCGGGAAAT 
CCAATTTTCA ACGACATTTG GACGTCTATT GCTAGTTGGA CAGAAAGAAG GCTTCAGAGT 
TGTTCCCAGA TAGAAGTGAC CTTACCTTGT GTTGAATGTT TCTTTAAGTA TAAAAAAGAT 
GCTTTGCATA CTCTTTTACC AGGTTTTATG CGTTGCTTCC ATAAAGTTGC CAAGGAGTTT 
CTTTCGTTAG GAAGTCAGCC ATCAGGAAAC TCACTTAACC TTCAGATTGT TAATGCTGTT 
GATGAACGAG TATCTATTTT AAAGTCAATG ATCGAGTTGG GTTGTTCTTA CATCAGCTAT 
CTTGGTGATC AAAGACGATG GTTTTTAAGT GCCTTGGTAC AAATAATTGA AAAATCCTCA 
AGCTACGAAA TTTGTAATTA TCTATTGGAA ATTGTGCGAG GTTGGATTAT GAATTCTCCG 
GTTCCTGTTC CAACGGTGAA AGAGAAAGCT GCTTTACTAT TAAAAATGGT AACCTTTGAA 
GGAAGATTCA GCCAAAATGA ACAAAATGAT TTATTTAACA AATATTTATC GTTTATAGCT 
GATATTTACG AAATGGAGCC ATACAAAAAT AGTGAGTTGA CGTTTCGATT AGAAGCAGTG 
TATCTTCTAG GTACTCGAGT TGCGAATAAG AAATTGAAGG AAAGGTTTAT AAAAGGCTTA 
AATTCTAGTT TCCCATCTGA TCTATTTTCT CGTTTTCAAT TTCTCTTGGG AAGTCAACAT 
TGGGAATCAC TTTCTAATAC TTACTGGATC GTTCAATTGA ACATTTTTTT AAGCCGTTGC 
TTTGATTTAA ACCAAAGATG TCAATTTTAT AAAAAGCCCA AGTTATTTTC TTGTTTTTCT 
ATCTACTGTC GAGAATTCGA TGAGGATTTG ACTTCCCAAG CGCAAGATAC CGAAATGTTA 
CATAACAATT TACTAAAGTA TGGAATAATT GATTTCAATC AAAACAGTAT GCTAGTCTCT 
GATTTTGTAC TTCCAGTTTT GAGCTTACAG TTTTCAAATT CAAAAATTGC GGAGTACTTA 
TGGCGAGATT TTTTTAATGC TTCAGTTTGC AGCTTTACAA AGGATGAAAT CCCTTTGTGT 
ATTGGTTCAA TAATTTCTTT TCTTTCTCGT GAATATCACA TCAGGCTTCT TGGGAAAACA 
CCTAATGTCT TGGAGACCAT TTTAACGTCG ATTGTATCCT CGGACATGCC TATACCATTA 
CCACCTCATC TTCTTGTATA TTTGAGTAAG ACATATGGAT TGCACCATTA TTGTATATTA 
TTACTAGAAA ATTCTTTGCA AAACAATCCA GGTCTTTCTG AAGATGAATT AACAGTGTAT 
CATAAAAGTT GTCTTGATGC TCTCAGCGAT ATCTATTATA GCTTGGATGA ACATGATTTG 
TACCATGGGC TTTGGAGAAG ACGTGCGAAT TTTTTGGAAA CCGAAGTTGC AACTTCTCAT 
GAACAATGCC ATGAGTGGGA AAAAGCTCAA TTGGTTTATG AGCATGCGCA ATTAAAAGTT 
TGTACAGGAA GTTTACCGTA TTCACCTACT GAACATGGAT TTTGGCTGGA TCATTGGATT 
TTATGTGCTC AAAAGCTAAA CCAATGGGAT GTACTTTTTG ATTTTTCAAA ACAAGAAGGA 
TGTGCTGAAC TTTATCTAGA ATGTGCATGG CGCTTATCTG ACTGGAGTAC CGAACAAGAT 
ACCTTAGAAA AGGCTACGAA GTCTTTATCT CCTTTCACAA GCCTTAGGCG GCATACTGCT 
GATGCGTTGT TATACCTAAA TAAAACCCAG AGGAAAATGG GATCTGTTAC AGAGTTTTCT 
AGAATAATTG ACGAGTGTAT GCAATTTTCG TTGAGGCGTT GGCAGCAGCT ACCCAAACGC 
GTGTATCAAT CGCATGTTTC TCTTCTACAT CACTTTCAGG AAATTGTAGA GTTACAGGAA 
GCTTTTGGTA TATACAGCCA GTTAAATGAT ACCAACATTC ACAATATTGA CAATAAGTTG 
CGGGATATAA AAGTCGTCCT TCAAGGTTGG AGGGAACGTT TGCCAAATGT TTGGGATGAT 
ATTGATATAT GGAGTGATTT AATAGCTTGG CGTCAGTCAG TTTTTAAATC AATCAACAAA 
GTGTTCCTTC CTCTTGTTTC AATTGCTCAA CAGTCAACAA ACAAGTCTAA TACAAACTCG 
GTTTCTTACC TTTATCGAGG ATACCACGAG CTGGCCTGGA TAATAAACCG CTTCGCTCAT 
GTTGCCAGAG TTCATCATTT ACCTGAAGTT TGCATCAATC AGCTGACAAA GATATATACG 
CTACCAAACA TCGAAATTCA AGAGGCTTTT TTGAAATTGA GAGAACAGGC TGAGTGTCAT 
TACGAATCTC CTTCTGAAAT GCAGCTGGGT TTGGAAGTCA TCAACAATAC AAACCTAATG 
TACTTTCGGA ATCGACAGAA GGCGGAATTT TTTACTCTAA AAGGGATGTT TCAAAACAGG 
CTTGGAGAAA AAGATGAAGC AAATCAGGCA TTTGCAACTG CTGTGCAAAT AGATATTGGT 
TCAGGAAAGG CTTGGAGTGA ATGGGGATTG TACCATGATG AACTATTTCA GGCAAACCCT 
CAAGAGATCC ATCATGCTTG TAATGCTGTG AGTTGTTTTT TACAAGCATC TTCTCTGTTG 
TCGAGTTCTA ACTCCAAACC CCTTTTAACA CGTGTACTTT GGTTATTGTC GGTAGATGAT 
TCTCACGGTT CTGTCTCAGA AGTGGTCTCA TCCTTTAAGT CGGAAATTCC TACTTGGAAC 
TGGATTCCGT TTATTCCACA GCTTTTGTCG GCCTTATCAC ATCGTGAATC AATCCATGCA 
AGAGCAATTC TTATTCAAAT TGCTAAAACA TATCCCCAAT CTCTTCATTT TCAGCTTCGT 
ACCGCGTATG AAGACTTCCT AATGCTTAAG AAGCAACAGG CGGCAAATGT ACTAAGGGGA 
AATAGTCGTT TACGTGAAAA CGATTCTTCT TCGGATAATA AATCAAAAGA TCTCTCTCCT 
TCTGGCTCGT TTTCAAGCGT TAGCCAATTT AACAGTAAGA ATGGAAGCCC TTCCTCTATA 
GACTCTTCTG AGAAACACCA AATATCAACC GTCAAACCTG CGTGGGAGCT AATTGCTGAT 
GTGACATCTA TCCTTAAAAC CGCATATCCG TTGCTGGCTC TTACTATGGA AACTATGGTC 
GACCAAATAC ATACCCGACT AAAATCATTT CCTGAAGAGG ATGCCTACAG GCTCATTGTC 
GCTCTTCTAA ATGATGGCCT TCAGTACATA TCTCGTTTAG GCGTTGTATC AAAAAATACA 
TTCCAGCTAC CTATGTCTCA AGCCAACATT CAACGATTTG CTGAAAACGT TCTTCCTGTA 
TCAGTGCGAG AAGCATTTTT ACGGGATTTT GTTGAAACAA AATTAGACTT ATTAACATAT 
GTTGACAAAT TACGTATGTG GAGAAAGAAA TTTGAAAACA TACTGGATCA AAGACCTAAA 
TTTCTTCATT TGGAACAGTG TAGTTTATAC TTGAGTGAAT TTCAACATCA AAAGTTTGAC 
GAAGTTGAGA TTCCTGGTCA ATATTTGCTC GATAAAAATA ACAATAATGA TTTTGTACGA 
TTGGAACGTT TTGTTCCAAA CGTTGATTTG GTACGAGGCC ATACTATGTG TTATAAGCGA 
CTTACTCTTA GAGGATACGA TGGAAAACTT TATCCTTTTG CATTACAATA TCCTGCTACT 
CGACACAGTC GACGTGAAGA GCGCATGCTT CAGTTGCTTG GCACGTTTAA TACTGTATTA 
CGCTCTAAAA TAGAAATTCA GAATCGAAAT TTCAGTTTTC AAATACCGTC TTCAATTCCT 
TTATCATCAC ATATGCGGAT AATTGCTGAT AAGCCTTCTT ATGTTACAAT GCAAACTATA 
AGTGATGAGT ACTGCAAAAA TAGGGGGATG CCTTTAGATT ATGGAATTCG TTTTTATTTT 
GATAGATTGC AGACTGGTCT TATTCAATTG AAGCGAGCTA GTGCTTCTAT GCTTAGCAAT 
AGTACGGTGG AAGAGAAAAA GCAAATATTT CGTCAACGAG CGTTGCAGTT GCGTATGCAA 
TTACTCGAAA CACTGAATTC ATCAGTTTTC CCTGAAAGCA TATACTATGA CTACTTTTAT 
AAAACCTTTG AAAGGTACTG TGACTTTTGG TTTTTCCGCA GGACATTTAC AACGCAATAT 
GCTTACATGA TTATTATGAC TTATGTATTC AATATTGGAG GTCGATCCCC TCAAAAGCTG 
TTCATAGTGA AGGATTCTGG CCAGGTGATG TCGCAAGACC TTTTACCTTC TATGACTTCC 
AACCAACCTG TATTTCACAA TACTGAGGCT GTTCCGTTTA GATTAACGCC TCCAATACAA 
TACCTCATTT CTGATTTGGG AGTTGAAGGA TTACTTTCGG GATTAGTAAT GAGCATTGCG 
CAAAGTCTAT CAAGTCCTAC TACTGATATA AAGCAGTATC TAAGTCTTTA TGTTCGTGAT 
GAAGTTTTCT GGTGGTCCAA GCAGCAAAGA AAGCCAATTC CTCAGGGAAT CCAGCTGTTC 
GAAACCGTGA AAGTAAATGT TGAATTATTA TTTCGTCGAA TATCGGTCAT ATCTCATAAT 
GTACCAGAGG ACCTTCCACT GAATCAGACT TTGGTGGATT TAGTATCTCA AGCAACTAAT 
CCTCAGCAGC TGGCGCAAAT GGATCAACTT TGGCAAGCTT GGCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_592862.1
CDS	117..11084
Translation	MEDGFEKSLSSSIELLKAESTEIEEKIKLTKILITKVSSISSDASQLYAELIPLLLDFLRNTEVNLYRNSSVNELKIEALTLIQSCAHRDEFKQYAQSCVLSFISLIKADNEEVAVFCLKVIMDIFKTFKFCIESTAQPFFDLVLELSTNLPYLIPSIFVENPKSNEEENTTLAFGSYLSTETSIIQQRVNSLAISTQPLELASQSFRVYVECPVIIVLILQAYRQAAFPGVQAIIPCFLKMVQIDVPIDIASYAMIEKDSSIDFIEFIRNKYQYRNFFMAQVKTLSFLAYILRTHPNTLSEKDIIPDIVIKLLRRCPFDMCFARKELLVATRHILSTNLKSLFVKKLDFLLDLNILLGNGVGTQKLLRPLAFSTLADLLHHVRDELNETQIRKSIMIYSTNMHDLTLSIGLQTMGARLILNMVDRMISLPSIPDAIFLLLSIFDSFVNKFSELNDSLDQFFKKKYEEEIKETKSPTRSSPRDLSSFSTSVNDGSFLFKNLMFGLRALMYGLRTCKSRCIEIGGEQFSGFLTNIKPFEAVTFQKLFFEVGKGFSYFRPEQVYLETFFCCEEESLDRPAISTLPRNKDEKDCLEVFATIFIHLEPSIFLKVFETNLPTFFDQLKKNLTLFHIPQFLLSNESTSSKFLNILLRFLLSRIEELGSSDIRHGSVLLRLFRLSFVTVSMFATENEPVLRPYVSEIIVKCMKLAPNSANSLNYYYLLRALFRGIGGGRFESLYKEVMPLLHALLEAFNSLLISARTPKEKDLFTELCLTIPVRLSLLLPYMSYLMRPLVMSLKSSQELVSQGLRTFELCLDNLTPDFLDPIMAPYIEDLMNALWSHLQPLPYNYNHSHTALKILGKLGGRNRKLLDRVQSLKNSPEPNNDFTLLLSIKGVKQPQLLHYTQYVDEAVNLLSSPSSDLEVKQQAFTYVCNISKLYVYKSDATNSLASSIRCTADKISKSNFDFRRPYSVIPSRMTGRSSFTQLSDDSDETIILASATYGLFFATTVDELREEAYFWLEKLAVNVIVHDIFYAFDVIQGNHSKFTTNLQKEVIISPHYFAHCLSEVVCNDNSKMGDAVKHVLKFMFSFLESIFENPERAFILPIFEVLLSDFRHKCYDPHWYKKYGGCFGLECLIEQDHSSKWLFDRQVDILTALFFTLKDTTSEVPTVCKDHVMDVLKQLFRKIYASKDTEIAPGILGHLVLELSNHNSVVRSSTQKLLSLLSELSNTPIVKLVSPFKERLLSPIFAKPLRALPFHIQIGHIDAVNYCISLGSELITFSDELIRLIHETTALAEADDDALIGIGKTSHFKNAALLIRLRVVCVELLSTCILKIDFNNPQHAHLREGIIVVFFKSLYAKSKELIEAASLGLKNALQEDQKLSKELLQTTLRPILYNISDYRRLSVAGLEGLGRLLRLLTNYFKVEIGRKLLDHLKALLENVDFQKVSSLPLFCQTEMKIVRALIDLFHLLPNNANRFMDELLICVVEFSLRLQRTFPNYFSEPLLKYVNRYPEDAWKFFMVRYDEAAFTSVFVELLRLKDSDPLLSVVKDNWLFFQTILTNEINTVTANRYSFALDSAIVILQRDPSFFKDKNDFFRGSMDAVLAISHLVENESILESMVFWNDLLVRTSTFLLEVYDLCIYNYDDGLKLLSCFHMYKNSLAKNLVSDLTAHLVKKIEEPDLENNVKLILNLILSKDYGFLLKENLAGILLTYLNQNVSSLEKCNQIFSIFYEVFFQHPSTNVYANDEGIKIGALQIISFFLKNVPEITVQHQTEMLKMCSLFGNSEDVMIKQLSIYVMSLFILRSQFPYELVNVVYMALLKSSPIEVRHLVKSSFDNIFSYIFSEEPESKKSPIWFELPLQVISSQSQNISQLLNVYDFISSHSDIFIEHRGRYVPILIDSLYKFGAIPNPNPEIRALSLGLIKVLLEWNDLQLKVDQKEIFSNNQKRAILSYLFRFVCLFSEPFTEGLCSEAISLLERLLSSGTWASLGMKLSFFTKSITHFDATDANSVMFANSLRTLSIVVGHSDSAWIEENLSDLKFLLEKSLENESVGVQSAIGNFVSTILTLSNTHPSIAGNPIFNDIWTSIASWTERRLQSCSQIEVTLPCVECFFKYKKDALHTLLPGFMRCFHKVAKEFLSLGSQPSGNSLNLQIVNAVDERVSILKSMIELGCSYISYLGDQRRWFLSALVQIIEKSSSYEICNYLLEIVRGWIMNSPVPVPTVKEKAALLLKMVTFEGRFSQNEQNDLFNKYLSFIADIYEMEPYKNSELTFRLEAVYLLGTRVANKKLKERFIKGLNSSFPSDLFSRFQFLLGSQHWESLSNTYWIVQLNIFLSRCFDLNQRCQFYKKPKLFSCFSIYCREFDEDLTSQAQDTEMLHNNLLKYGIIDFNQNSMLVSDFVLPVLSLQFSNSKIAEYLWRDFFNASVCSFTKDEIPLCIGSIISFLSREYHIRLLGKTPNVLETILTSIVSSDMPIPLPPHLLVYLSKTYGLHHYCILLLENSLQNNPGLSEDELTVYHKSCLDALSDIYYSLDEHDLYHGLWRRRANFLETEVATSHEQCHEWEKAQLVYEHAQLKVCTGSLPYSPTEHGFWLDHWILCAQKLNQWDVLFDFSKQEGCAELYLECAWRLSDWSTEQDTLEKATKSLSPFTSLRRHTADALLYLNKTQRKMGSVTEFSRIIDECMQFSLRRWQQLPKRVYQSHVSLLHHFQEIVELQEAFGIYSQLNDTNIHNIDNKLRDIKVVLQGWRERLPNVWDDIDIWSDLIAWRQSVFKSINKVFLPLVSIAQQSTNKSNTNSVSYLYRGYHELAWIINRFAHVARVHHLPEVCINQLTKIYTLPNIEIQEAFLKLREQAECHYESPSEMQLGLEVINNTNLMYFRNRQKAEFFTLKGMFQNRLGEKDEANQAFATAVQIDIGSGKAWSEWGLYHDELFQANPQEIHHACNAVSCFLQASSLLSSSNSKPLLTRVLWLLSVDDSHGSVSEVVSSFKSEIPTWNWIPFIPQLLSALSHRESIHARAILIQIAKTYPQSLHFQLRTAYEDFLMLKKQQAANVLRGNSRLRENDSSSDNKSKDLSPSGSFSSVSQFNSKNGSPSSIDSSEKHQISTVKPAWELIADVTSILKTAYPLLALTMETMVDQIHTRLKSFPEEDAYRLIVALLNDGLQYISRLGVVSKNTFQLPMSQANIQRFAENVLPVSVREAFLRDFVETKLDLLTYVDKLRMWRKKFENILDQRPKFLHLEQCSLYLSEFQHQKFDEVEIPGQYLLDKNNNNDFVRLERFVPNVDLVRGHTMCYKRLTLRGYDGKLYPFALQYPATRHSRREERMLQLLGTFNTVLRSKIEIQNRNFSFQIPSSIPLSSHMRIIADKPSYVTMQTISDEYCKNRGMPLDYGIRFYFDRLQTGLIQLKRASASMLSNSTVEEKKQIFRQRALQLRMQLLETLNSSVFPESIYYDYFYKTFERYCDFWFFRRTFTTQYAYMIIMTYVFNIGGRSPQKLFIVKDSGQVMSQDLLPSMTSNQPVFHNTEAVPFRLTPPIQYLISDLGVEGLLSGLVMSIAQSLSSPTTDIKQYLSLYVRDEVFWWSKQQRKPIPQGIQLFETVKVNVELLFRRISVISHNVPEDLPLNQTLVDLVSQATNPQQLAQMDQLWQAWL

Target ORF information:

RefSeq Version	NM_001018262.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe phosphatidylinositol kinase-like protein Tra2 (tra2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018262.2

ORF Insert Sequence:

ATGGAAGACG GTTTTGAAAA GTCGCTCAGC AGCTCTATAG AGCTACTGAA AGCTGAAAGT 
ACAGAAATAG AAGAAAAAAT CAAACTAACT AAAATTTTAA TTACTAAGGT TTCTAGCATA 
TCTTCAGACG CCTCGCAGCT GTATGCAGAA TTAATTCCAC TACTCTTGGA TTTTTTACGA 
AATACAGAGG TGAATCTTTA TAGAAATTCT TCTGTCAATG AACTCAAGAT CGAAGCATTG 
ACTTTAATAC AAAGTTGCGC TCACCGTGAT GAATTTAAAC AATATGCGCA GTCTTGTGTG 
CTTTCGTTCA TTAGCTTGAT TAAAGCAGAT AATGAAGAAG TCGCTGTTTT TTGCTTAAAA 
GTTATTATGG ATATATTCAA AACTTTTAAG TTTTGTATAG AAAGCACTGC ACAACCCTTT 
TTTGACTTGG TACTTGAGCT TTCTACAAAC CTTCCTTATT TAATTCCCTC AATATTTGTC 
GAGAATCCAA AATCAAATGA AGAGGAAAAT ACAACCCTGG CTTTTGGTAG TTATCTGTCT 
ACGGAAACTT CAATCATCCA ACAGCGTGTC AATTCTTTAG CTATTTCTAC TCAACCTTTG 
GAACTGGCTT CTCAAAGTTT CCGCGTGTAT GTAGAGTGTC CGGTCATTAT AGTTTTGATT 
CTTCAAGCAT ATCGTCAAGC TGCTTTCCCT GGTGTTCAAG CCATAATTCC CTGTTTTCTT 
AAGATGGTAC AAATTGATGT TCCAATAGAC ATCGCTTCAT ATGCAATGAT TGAAAAAGAT 
TCAAGCATTG ACTTTATTGA GTTTATAAGG AATAAATATC AGTACCGTAA TTTTTTTATG 
GCTCAAGTAA AGACTTTATC TTTCTTGGCT TATATTTTAC GAACTCATCC CAATACACTT 
TCTGAGAAGG ATATAATACC GGACATTGTA ATAAAGCTTT TGAGGCGATG CCCTTTTGAT 
ATGTGTTTTG CCCGTAAAGA ACTGCTGGTT GCTACGAGAC ATATTCTGTC AACAAACTTA 
AAATCTCTTT TTGTCAAAAA GCTGGATTTT TTGTTAGATT TAAATATATT ATTAGGAAAT 
GGAGTTGGTA CTCAAAAGCT GTTACGTCCC TTGGCATTTA GTACATTAGC TGACTTACTT 
CATCATGTAC GTGACGAACT TAACGAAACG CAAATAAGAA AATCTATAAT GATTTATTCG 
ACCAATATGC ATGATTTGAC TCTTAGCATT GGGTTACAGA CTATGGGTGC CCGGCTCATT 
TTAAACATGG TTGACAGAAT GATTAGTCTT CCTAGCATCC CCGATGCTAT ATTTCTTCTT 
TTATCTATTT TTGATAGCTT TGTAAATAAG TTTTCGGAAC TTAATGATAG TTTGGATCAG 
TTTTTTAAGA AAAAGTATGA AGAAGAAATT AAAGAGACAA AGTCACCGAC GCGTTCTTCT 
CCTAGGGATT TATCTTCATT TAGCACTTCC GTCAATGATG GATCATTTCT TTTTAAAAAC 
TTGATGTTTG GCTTACGGGC TCTTATGTAT GGGCTTCGTA CATGCAAGTC TCGATGCATA 
GAAATTGGAG GTGAACAATT TTCGGGATTC CTTACGAATA TCAAGCCTTT CGAAGCTGTT 
ACGTTTCAGA AGCTTTTTTT TGAGGTTGGC AAAGGATTCT CATATTTTCG ACCTGAGCAA 
GTCTATTTAG AAACTTTTTT CTGCTGCGAA GAAGAAAGTT TAGATCGCCC TGCTATATCT 
ACGCTTCCTC GTAACAAAGA CGAGAAAGAT TGTCTTGAAG TATTTGCTAC CATTTTTATA 
CATCTCGAGC CATCTATATT TCTTAAAGTT TTTGAAACAA ACCTTCCTAC TTTTTTTGAT 
CAACTTAAAA AAAATCTCAC ATTATTTCAC ATTCCACAGT TTTTGCTTTC AAATGAGAGC 
ACATCATCAA AATTTTTAAA TATTTTACTT CGATTTCTGC TTTCCAGAAT TGAGGAACTC 
GGATCTTCTG ACATCAGGCA TGGTTCTGTC TTGTTGCGTT TGTTTCGTCT TTCTTTCGTT 
ACAGTCTCAA TGTTTGCAAC AGAGAATGAG CCAGTTCTTC GGCCTTATGT ATCTGAAATT 
ATTGTGAAAT GTATGAAGTT GGCTCCCAAT TCTGCTAATT CATTGAACTA CTATTATCTA 
TTAAGAGCGT TGTTCCGAGG AATTGGTGGG GGAAGATTTG AAAGCCTTTA TAAAGAAGTA 
ATGCCTTTGT TGCACGCTCT ATTAGAGGCA TTTAATTCAT TGCTTATATC TGCCCGTACT 
CCGAAGGAGA AAGACCTTTT TACCGAACTT TGTCTTACTA TTCCTGTAAG GCTTAGTCTT 
TTATTACCAT ACATGAGTTA TCTAATGAGA CCTCTCGTCA TGTCTTTAAA AAGCAGTCAA 
GAATTAGTTA GTCAGGGATT ACGAACCTTT GAATTATGCT TGGATAATCT CACTCCTGAT 
TTTTTGGATC CGATAATGGC TCCTTACATA GAAGATCTAA TGAATGCTTT ATGGAGTCAT 
CTACAACCTC TTCCTTATAA TTATAATCAC AGCCATACTG CCTTGAAAAT ACTTGGTAAA 
CTTGGTGGAA GAAATAGGAA GCTTCTTGAT AGAGTCCAGA GTTTAAAGAA TTCGCCGGAA 
CCGAATAACG ATTTTACTTT ACTTCTTTCG ATAAAAGGGG TTAAACAACC ACAACTTTTA 
CATTACACCC AATATGTTGA TGAGGCTGTG AATTTGCTTT CTAGCCCTTC TAGTGATCTG 
GAGGTTAAGC AACAAGCGTT CACTTATGTT TGCAATATTT CAAAGCTCTA TGTTTATAAG 
AGTGACGCTA CTAACAGCTT AGCCTCATCC ATTCGATGTA CTGCAGACAA GATTTCTAAG 
AGTAATTTTG ATTTTCGACG ACCATATTCT GTAATACCGA GCCGAATGAC TGGAAGGAGT 
TCATTCACCC AACTGAGTGA TGATTCAGAT GAAACCATAA TTTTGGCCTC GGCTACATAT 
GGACTTTTTT TTGCTACGAC GGTTGATGAA CTCCGCGAAG AAGCTTACTT CTGGCTTGAA 
AAACTGGCGG TCAACGTAAT TGTACACGAT ATTTTTTATG CTTTTGACGT TATTCAAGGA 
AATCATTCAA AATTTACGAC TAATTTGCAA AAGGAGGTTA TTATTAGTCC TCATTATTTT 
GCTCATTGTC TATCAGAAGT CGTTTGTAAT GACAATAGCA AGATGGGTGA TGCTGTGAAA 
CATGTACTTA AATTTATGTT TAGTTTTTTA GAATCGATTT TTGAAAATCC AGAAAGGGCT 
TTTATCCTTC CAATTTTTGA GGTCTTATTA TCTGATTTCC GGCACAAATG TTATGACCCT 
CATTGGTATA AAAAATACGG AGGCTGTTTT GGATTGGAAT GTTTGATAGA GCAAGATCAT 
TCTTCTAAAT GGCTTTTTGA CAGACAAGTT GATATTTTAA CTGCGCTATT TTTTACATTG 
AAAGATACTA CCTCAGAAGT CCCTACTGTC TGTAAGGATC ACGTGATGGA CGTTTTAAAG 
CAGTTATTTC GAAAAATATA TGCTTCCAAA GATACTGAAA TTGCTCCCGG AATTTTAGGG 
CATCTAGTAT TGGAATTGTC AAATCATAAT TCTGTGGTCC GTTCTTCTAC CCAAAAATTG 
CTTTCTTTAT TGTCGGAACT TTCAAATACT CCGATTGTTA AACTAGTTTC ACCTTTCAAA 
GAAAGACTTC TCTCACCAAT ATTTGCAAAA CCTCTTCGAG CATTACCTTT CCATATACAA 
ATTGGACATA TCGATGCTGT AAATTACTGC ATAAGTCTTG GAAGTGAATT GATTACTTTT 
TCCGACGAAT TAATTCGTCT TATTCATGAA ACCACTGCTT TAGCAGAAGC GGACGATGAT 
GCTCTTATTG GAATTGGTAA AACTTCTCAC TTCAAAAACG CTGCACTTCT TATACGTTTG 
AGAGTAGTAT GTGTAGAATT ACTCAGTACT TGCATTTTGA AGATTGATTT TAATAACCCT 
CAACATGCAC ACTTACGTGA AGGAATTATT GTTGTTTTTT TTAAATCTTT GTATGCAAAG 
TCCAAGGAGC TTATTGAAGC GGCATCATTA GGTCTCAAGA ACGCTTTGCA AGAGGACCAA 
AAGTTATCAA AAGAATTGTT ACAAACAACT TTGCGTCCAA TACTGTATAA TATATCTGAC 
TATCGAAGGT TAAGCGTTGC AGGACTTGAA GGTTTGGGAA GGCTGCTACG CCTCCTCACA 
AATTACTTTA AGGTCGAGAT AGGAAGAAAA TTACTAGATC ATTTAAAGGC TTTACTTGAA 
AATGTTGATT TCCAAAAGGT GTCTAGTCTT CCTTTGTTTT GTCAAACTGA AATGAAAATT 
GTTCGAGCTC TTATTGATCT TTTTCATTTA TTACCAAATA ACGCAAATAG ATTTATGGAT 
GAATTGTTAA TTTGTGTTGT AGAATTTAGT CTGAGACTAC AAAGAACCTT TCCTAATTAT 
TTCAGTGAAC CTTTGCTAAA ATATGTCAAT AGATATCCCG AAGATGCTTG GAAATTTTTT 
ATGGTTAGAT ACGATGAGGC CGCTTTTACG TCTGTTTTTG TTGAGTTACT TCGACTTAAA 
GATTCAGACC CCCTACTTTC AGTTGTGAAG GATAATTGGT TATTTTTTCA GACCATTTTA 
ACTAACGAAA TCAATACTGT AACTGCTAAC CGCTATTCAT TTGCTTTGGA CTCTGCCATT 
GTAATACTAC AACGTGATCC TTCTTTCTTC AAAGATAAAA ATGACTTTTT TAGAGGCTCG 
ATGGATGCCG TGCTAGCAAT AAGCCATCTG GTTGAAAATG AGTCCATTTT GGAATCCATG 
GTTTTTTGGA ACGACCTGTT GGTTCGTACC TCAACATTTT TGTTGGAAGT GTATGATTTA 
TGCATATATA ATTATGACGA TGGGCTCAAG TTACTGTCAT GCTTTCACAT GTATAAAAAC 
TCATTGGCCA AGAACCTTGT AAGTGACCTT ACGGCTCATT TGGTAAAAAA AATTGAAGAA 
CCTGATTTAG AGAACAATGT TAAATTGATT TTAAACCTTA TTTTATCAAA AGACTACGGG 
TTTTTATTGA AGGAAAACTT GGCTGGCATT CTTCTCACGT ATCTCAATCA GAATGTGTCT 
TCTCTTGAAA AATGTAATCA AATATTTTCT ATATTTTACG AAGTTTTTTT CCAACATCCT 
TCCACTAATG TTTATGCTAA TGATGAGGGA ATAAAAATAG GCGCTTTACA AATAATTTCT 
TTTTTTTTAA AAAATGTCCC AGAAATTACT GTACAGCATC AGACTGAAAT GCTTAAGATG 
TGCAGTCTCT TTGGAAATTC AGAGGACGTG ATGATTAAAC AATTATCGAT TTATGTCATG 
TCTTTATTCA TTTTACGATC ACAATTTCCG TACGAGCTAG TAAACGTTGT GTATATGGCT 
CTTTTGAAAA GTTCCCCTAT TGAAGTTCGA CATTTGGTAA AATCTTCTTT TGACAACATA 
TTTTCGTACA TATTTTCTGA GGAGCCAGAA AGCAAGAAAT CACCTATCTG GTTTGAATTG 
CCTTTACAAG TTATTAGCAG CCAAAGTCAA AATATTTCAC AGCTGCTCAA TGTTTACGAT 
TTTATTAGTA GTCACTCCGA TATTTTTATC GAACACAGGG GAAGGTATGT TCCGATTTTA 
ATTGATTCTT TATACAAATT TGGAGCCATT CCGAACCCTA ATCCTGAGAT TCGCGCTCTA 
TCTTTAGGCT TAATTAAGGT TTTACTAGAA TGGAATGATC TCCAACTTAA AGTTGACCAG 
AAGGAAATTT TTTCAAATAA CCAGAAGAGA GCTATATTGA GTTACCTATT TCGTTTTGTG 
TGCCTTTTTT CTGAACCCTT CACAGAAGGT TTGTGTTCCG AAGCTATTTC TTTACTAGAG 
CGTTTGCTTT CATCCGGTAC ATGGGCAAGT TTAGGGATGA AACTATCTTT TTTTACGAAG 
AGTATTACTC ATTTTGATGC TACTGATGCC AATTCAGTGA TGTTTGCCAA TTCGCTAAGA 
ACCCTGTCAA TCGTGGTGGG GCACTCAGAT TCTGCGTGGA TAGAAGAAAA CCTTTCCGAT 
TTGAAGTTTT TATTAGAAAA ATCTTTAGAA AATGAATCAG TTGGTGTACA GAGTGCAATT 
GGAAATTTTG TTTCAACTAT ACTAACGCTC TCCAACACTC ATCCTTCAAT TGCGGGAAAT 
CCAATTTTCA ACGACATTTG GACGTCTATT GCTAGTTGGA CAGAAAGAAG GCTTCAGAGT 
TGTTCCCAGA TAGAAGTGAC CTTACCTTGT GTTGAATGTT TCTTTAAGTA TAAAAAAGAT 
GCTTTGCATA CTCTTTTACC AGGTTTTATG CGTTGCTTCC ATAAAGTTGC CAAGGAGTTT 
CTTTCGTTAG GAAGTCAGCC ATCAGGAAAC TCACTTAACC TTCAGATTGT TAATGCTGTT 
GATGAACGAG TATCTATTTT AAAGTCAATG ATCGAGTTGG GTTGTTCTTA CATCAGCTAT 
CTTGGTGATC AAAGACGATG GTTTTTAAGT GCCTTGGTAC AAATAATTGA AAAATCCTCA 
AGCTACGAAA TTTGTAATTA TCTATTGGAA ATTGTGCGAG GTTGGATTAT GAATTCTCCG 
GTTCCTGTTC CAACGGTGAA AGAGAAAGCT GCTTTACTAT TAAAAATGGT AACCTTTGAA 
GGAAGATTCA GCCAAAATGA ACAAAATGAT TTATTTAACA AATATTTATC GTTTATAGCT 
GATATTTACG AAATGGAGCC ATACAAAAAT AGTGAGTTGA CGTTTCGATT AGAAGCAGTG 
TATCTTCTAG GTACTCGAGT TGCGAATAAG AAATTGAAGG AAAGGTTTAT AAAAGGCTTA 
AATTCTAGTT TCCCATCTGA TCTATTTTCT CGTTTTCAAT TTCTCTTGGG AAGTCAACAT 
TGGGAATCAC TTTCTAATAC TTACTGGATC GTTCAATTGA ACATTTTTTT AAGCCGTTGC 
TTTGATTTAA ACCAAAGATG TCAATTTTAT AAAAAGCCCA AGTTATTTTC TTGTTTTTCT 
ATCTACTGTC GAGAATTCGA TGAGGATTTG ACTTCCCAAG CGCAAGATAC CGAAATGTTA 
CATAACAATT TACTAAAGTA TGGAATAATT GATTTCAATC AAAACAGTAT GCTAGTCTCT 
GATTTTGTAC TTCCAGTTTT GAGCTTACAG TTTTCAAATT CAAAAATTGC GGAGTACTTA 
TGGCGAGATT TTTTTAATGC TTCAGTTTGC AGCTTTACAA AGGATGAAAT CCCTTTGTGT 
ATTGGTTCAA TAATTTCTTT TCTTTCTCGT GAATATCACA TCAGGCTTCT TGGGAAAACA 
CCTAATGTCT TGGAGACCAT TTTAACGTCG ATTGTATCCT CGGACATGCC TATACCATTA 
CCACCTCATC TTCTTGTATA TTTGAGTAAG ACATATGGAT TGCACCATTA TTGTATATTA 
TTACTAGAAA ATTCTTTGCA AAACAATCCA GGTCTTTCTG AAGATGAATT AACAGTGTAT 
CATAAAAGTT GTCTTGATGC TCTCAGCGAT ATCTATTATA GCTTGGATGA ACATGATTTG 
TACCATGGGC TTTGGAGAAG ACGTGCGAAT TTTTTGGAAA CCGAAGTTGC AACTTCTCAT 
GAACAATGCC ATGAGTGGGA AAAAGCTCAA TTGGTTTATG AGCATGCGCA ATTAAAAGTT 
TGTACAGGAA GTTTACCGTA TTCACCTACT GAACATGGAT TTTGGCTGGA TCATTGGATT 
TTATGTGCTC AAAAGCTAAA CCAATGGGAT GTACTTTTTG ATTTTTCAAA ACAAGAAGGA 
TGTGCTGAAC TTTATCTAGA ATGTGCATGG CGCTTATCTG ACTGGAGTAC CGAACAAGAT 
ACCTTAGAAA AGGCTACGAA GTCTTTATCT CCTTTCACAA GCCTTAGGCG GCATACTGCT 
GATGCGTTGT TATACCTAAA TAAAACCCAG AGGAAAATGG GATCTGTTAC AGAGTTTTCT 
AGAATAATTG ACGAGTGTAT GCAATTTTCG TTGAGGCGTT GGCAGCAGCT ACCCAAACGC 
GTGTATCAAT CGCATGTTTC TCTTCTACAT CACTTTCAGG AAATTGTAGA GTTACAGGAA 
GCTTTTGGTA TATACAGCCA GTTAAATGAT ACCAACATTC ACAATATTGA CAATAAGTTG 
CGGGATATAA AAGTCGTCCT TCAAGGTTGG AGGGAACGTT TGCCAAATGT TTGGGATGAT 
ATTGATATAT GGAGTGATTT AATAGCTTGG CGTCAGTCAG TTTTTAAATC AATCAACAAA 
GTGTTCCTTC CTCTTGTTTC AATTGCTCAA CAGTCAACAA ACAAGTCTAA TACAAACTCG 
GTTTCTTACC TTTATCGAGG ATACCACGAG CTGGCCTGGA TAATAAACCG CTTCGCTCAT 
GTTGCCAGAG TTCATCATTT ACCTGAAGTT TGCATCAATC AGCTGACAAA GATATATACG 
CTACCAAACA TCGAAATTCA AGAGGCTTTT TTGAAATTGA GAGAACAGGC TGAGTGTCAT 
TACGAATCTC CTTCTGAAAT GCAGCTGGGT TTGGAAGTCA TCAACAATAC AAACCTAATG 
TACTTTCGGA ATCGACAGAA GGCGGAATTT TTTACTCTAA AAGGGATGTT TCAAAACAGG 
CTTGGAGAAA AAGATGAAGC AAATCAGGCA TTTGCAACTG CTGTGCAAAT AGATATTGGT 
TCAGGAAAGG CTTGGAGTGA ATGGGGATTG TACCATGATG AACTATTTCA GGCAAACCCT 
CAAGAGATCC ATCATGCTTG TAATGCTGTG AGTTGTTTTT TACAAGCATC TTCTCTGTTG 
TCGAGTTCTA ACTCCAAACC CCTTTTAACA CGTGTACTTT GGTTATTGTC GGTAGATGAT 
TCTCACGGTT CTGTCTCAGA AGTGGTCTCA TCCTTTAAGT CGGAAATTCC TACTTGGAAC 
TGGATTCCGT TTATTCCACA GCTTTTGTCG GCCTTATCAC ATCGTGAATC AATCCATGCA 
AGAGCAATTC TTATTCAAAT TGCTAAAACA TATCCCCAAT CTCTTCATTT TCAGCTTCGT 
ACCGCGTATG AAGACTTCCT AATGCTTAAG AAGCAACAGG CGGCAAATGT ACTAAGGGGA 
AATAGTCGTT TACGTGAAAA CGATTCTTCT TCGGATAATA AATCAAAAGA TCTCTCTCCT 
TCTGGCTCGT TTTCAAGCGT TAGCCAATTT AACAGTAAGA ATGGAAGCCC TTCCTCTATA 
GACTCTTCTG AGAAACACCA AATATCAACC GTCAAACCTG CGTGGGAGCT AATTGCTGAT 
GTGACATCTA TCCTTAAAAC CGCATATCCG TTGCTGGCTC TTACTATGGA AACTATGGTC 
GACCAAATAC ATACCCGACT AAAATCATTT CCTGAAGAGG ATGCCTACAG GCTCATTGTC 
GCTCTTCTAA ATGATGGCCT TCAGTACATA TCTCGTTTAG GCGTTGTATC AAAAAATACA 
TTCCAGCTAC CTATGTCTCA AGCCAACATT CAACGATTTG CTGAAAACGT TCTTCCTGTA 
TCAGTGCGAG AAGCATTTTT ACGGGATTTT GTTGAAACAA AATTAGACTT ATTAACATAT 
GTTGACAAAT TACGTATGTG GAGAAAGAAA TTTGAAAACA TACTGGATCA AAGACCTAAA 
TTTCTTCATT TGGAACAGTG TAGTTTATAC TTGAGTGAAT TTCAACATCA AAAGTTTGAC 
GAAGTTGAGA TTCCTGGTCA ATATTTGCTC GATAAAAATA ACAATAATGA TTTTGTACGA 
TTGGAACGTT TTGTTCCAAA CGTTGATTTG GTACGAGGCC ATACTATGTG TTATAAGCGA 
CTTACTCTTA GAGGATACGA TGGAAAACTT TATCCTTTTG CATTACAATA TCCTGCTACT 
CGACACAGTC GACGTGAAGA GCGCATGCTT CAGTTGCTTG GCACGTTTAA TACTGTATTA 
CGCTCTAAAA TAGAAATTCA GAATCGAAAT TTCAGTTTTC AAATACCGTC TTCAATTCCT 
TTATCATCAC ATATGCGGAT AATTGCTGAT AAGCCTTCTT ATGTTACAAT GCAAACTATA 
AGTGATGAGT ACTGCAAAAA TAGGGGGATG CCTTTAGATT ATGGAATTCG TTTTTATTTT 
GATAGATTGC AGACTGGTCT TATTCAATTG AAGCGAGCTA GTGCTTCTAT GCTTAGCAAT 
AGTACGGTGG AAGAGAAAAA GCAAATATTT CGTCAACGAG CGTTGCAGTT GCGTATGCAA 
TTACTCGAAA CACTGAATTC ATCAGTTTTC CCTGAAAGCA TATACTATGA CTACTTTTAT 
AAAACCTTTG AAAGGTACTG TGACTTTTGG TTTTTCCGCA GGACATTTAC AACGCAATAT 
GCTTACATGA TTATTATGAC TTATGTATTC AATATTGGAG GTCGATCCCC TCAAAAGCTG 
TTCATAGTGA AGGATTCTGG CCAGGTGATG TCGCAAGACC TTTTACCTTC TATGACTTCC 
AACCAACCTG TATTTCACAA TACTGAGGCT GTTCCGTTTA GATTAACGCC TCCAATACAA 
TACCTCATTT CTGATTTGGG AGTTGAAGGA TTACTTTCGG GATTAGTAAT GAGCATTGCG 
CAAAGTCTAT CAAGTCCTAC TACTGATATA AAGCAGTATC TAAGTCTTTA TGTTCGTGAT 
GAAGTTTTCT GGTGGTCCAA GCAGCAAAGA AAGCCAATTC CTCAGGGAAT CCAGCTGTTC 
GAAACCGTGA AAGTAAATGT TGAATTATTA TTTCGTCGAA TATCGGTCAT ATCTCATAAT 
GTACCAGAGG ACCTTCCACT GAATCAGACT TTGGTGGATT TAGTATCTCA AGCAACTAAT 
CCTCAGCAGC TGGCGCAAAT GGATCAACTT TGGCAAGCTT GGCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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tra2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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