spo15 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	spo15
Entrez Gene ID	2541649
Full Name	sporulation protein Spo15
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018408.2	NP_593009.1	sporulation protein Spo15

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following spo15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spo15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc103912	NM_001018408.2 Latest version!	Schizosaccharomyces pombe sporulation protein Spo15 (spo15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spo15 ( NM_001018408.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593009.1 Schizosaccharomyces pombe sporulation protein Spo15 (spo15), mRNA.

Price & Availability↑

CloneID	OSc103912
Clone ID Related Accession (Same CDS sequence)	NM_001018408.2
Accession Version	NM_001018408.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	sporulation protein Spo15
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018408.1.

ATGTCGAATC AGTCGTCTTC TGGTTCGAAT ACTTCTGATT TGGATGAAGA ATCAGCGAGT 
AGTCTTGTGA GCTCTGCTGC ATCACCATTT ATTGATTCTG ATCTCGAAAC ACCAAGACCC 
AATATTTCAA GAGCTTCAAC AGGACAACTT GCAGAAGACG GTGACACATC AAGTCAACAT 
GAAGATTCCT CAGAGGAGTT AAAGCGTCAA GAAGTAAGGG GAATGCGTAG GCACAGTGAC 
CTTTCAATTG ATGCGAAATT AGGCTCTTCT GAAGGCTCAA CTGCTTCTTC AGCATTACCT 
TTGACACCTC GTTCTCCTTC TAATGCCAGC TGGCTATTAG TCCGCGGTGG TTTACTTGAT 
AGTCCGATTC TCGACATTAA CTCAGTAACT CAAAAATCCA ACCTTTTAAA TGAGTTGAAG 
CAAGTGCGGT CCAAACTGGC TGCCCTTGAA CATGAAAACG GAATTCTCTC CTTGCAATTG 
TCATCTTCGA ATAAAAAGGA TAAGAATACC TCAAGTGTAA CCACGTTAAC ATCAGAGGAA 
GACGTGTCAT ATTTTCAAAA AAAGCTCACT AACATGGAGT CAAATTTTTC TGCTAAACAA 
TCTGAAGCGT ACGATTTATC GCGACAGTTA TTAACGGTTA CGGAAAAATT AGATAAAAAA 
GAAAAGGATT ACGAAAAAAT AAAAGAAGAT GTTTCTTCTA TCAAGGCCTC TTTAGCAGAA 
GAGCAAGCTT CTAACAAGTC CCTGCGTGGT GAGCAAGAGC GATTAGAAAA ACTTCTTGTA 
AGCTCCAATA AAACTGTTAG TACCTTAAGA CAAACAGAAA ACTCATTGCG CGCCGAATGT 
AAAACCTTGC AAGAAAAGTT GGAGAAATGC GCCATCAATG AGGAAGATTC CAAATTGCTG 
GAAGAACTTA AGCATAATGT CGCCAATTAT TCAGATGCCA TAGTACACAA GGATAAGTTA 
ATTGAAGATT TAAGTACCAG AATAAGCGAG TTTGATAATT TAAAATCTGA ACGGGACACG 
TTGTCAATAA AAAACGAGAA GCTAGAAAAG TTACTACGTA ATACGATTGG CAGCCTAAAA 
GACTCACGAA CCTCCAATTC TCAATTGGAA GAGGAAATGG TTGAGTTGAA AGAATCCAAT 
CGTACTATCC ATTCGCAATT GACTGATGCT GAATCTAAGC TTTCTTCTTT TGAACAGGAA 
AATAAATCAC TGAAAGGATC TATTGACGAG TACCAAAATA ATCTTTCTTC GAAGGATAAA 
ATGGTTAAAC AAGTTTCTTC CCAATTGGAA GAAGCTCGTT CTTCCCTTGC CCACGCGACT 
GGTAAATTAG CAGAGATAAA TTCCGAGCGA GATTTTCAAA ATAAAAAAAT AAAAGATTTT 
GAAAAGATAG AGCAGGACTT GCGTGCTTGT TTGAATTCCT CATCCAATGA ACTGAAGGAA 
AAATCAGCGT TGATTGACAA AAAAGATCAA GAGTTAAATA ATTTGAGGGA GCAGATTAAA 
GAACAGAAAA AAGTTTCTGA AAGTACTCAA TCAAGCCTTC AGTCTTTGCA ACGTGATATT 
TTAAATGAAA AGAAAAAACA TGAAGTTTAT GAAAGTCAAT TAAACGAACT GAAGGGTGAA 
TTACAAACGG AAATTTCAAA TTCTGAACAC CTATCCTCCC AACTCTCAAC TTTGGCGGCT 
GAGAAAGAGG CAGCTGTGGC TACCAATAAT GAACTTTCCG AAAGTAAAAA CTCACTTCAG 
ACATTGTGCA ATGCTTTTCA AGAAAAGCTT GCAAAATCGG TTATGCAATT GAAAGAGAAT 
GAGCAAAACT TTTCTTCCCT GGATACTTCA TTTAAAAAAC TCAATGAAAG TCACCAAGAA 
TTAGAAAATA ACCACCAGAC CATAACCAAG CAGCTGAAAG ATACTTCTTC AAAACTTCAA 
CAGCTACAAT TAGAGCGAGC CAATTTCGAA CAAAAAGAAT CAACCTTGAG CGACGAAAAT 
AATGATCTGC GAACTAAATT ATTAAAGCTC GAGGAGTCCA ACAAATCGTT AATTAAGAAA 
CAGGAAGACG TGGATTCTTT AGAGAAAAAT ATTCAAACAT TGAAGGAAGA CTTGCGGAAG 
TCGGAGGAAG CCCTCCGATT TTCCAAATTG GAGGCTAAAA ATTTGAGAGA AGTGATAGAC 
AATTTGAAAG GAAAGCATGA GACATTGGAA GCACAGAGAA ATGATTTGCA TTCTTCTCTT 
TCTGATGCTA AAAATACGAA TGCAATATTG TCGAGTGAAC TTACCAAATC ATCTGAAGAT 
GTCAAGAGGT TGACTGCCAA CGTTGAAACT CTTACACAAG ATAGCAAAGC TATGAAGCAA 
AGTTTTACAA GCCTTGTCAA TTCTTATCAG TCAATCTCAA ACTTGTACCA CGAACTTCGT 
GATGACCATG TGAATATGCA ATCTCAAAAT AATACATTGC TTGAATCTGA ATCAAAATTG 
AAGACAGACT GTGAAAATCT CACTCAACAG AACATGACAC TCATTGACAA TGTTCAGAAA 
CTCATGCATA AACATGTAAA TCAAGAATCC AAAGTTTCTG AACTAAAAGA GGTGAATGGA 
AAGTTATCAT TAGATTTGAA GAATCTAAGA TCCAGTTTAA ATGTTGCTAT TTCTGACAAT 
GATCAAATCC TTACCCAACT TGCTGAACTT AGTAAAAATT ACGATAGTTT GGAGCAAGAG 
TCTGCTCAGT TAAATTCCGG TCTGAAATCA TTGGAAGCTG AAAAGCAATT GCTACATACA 
GAAAATGAGG AGCTACATAT TCGTTTGGAT AAGTTAACTG GCAAGTTAAA AATTGAAGAA 
TCGAAATCCA GTGATCTTGG TAAAAAATTA ACTGCACGGC AAGAAGAAAT TTCAAACTTG 
AAAGAAGAAA ACATGTCGCA AAGTCAAGCC ATAACTTCTG TGAAATCTAA ACTTGATGAA 
ACTTTGTCAA AGTCTAGCAA GCTTGAAGCA GATATTGAAC ACCTGAAAAA TAAGGTTTCC 
GAGGTTGAAG TTGAAAGGAA TGCTCTTCTA GCTTCAAATG AACGTTTAAT GGACGATTTG 
AAAAATAATG GTGAAAATAT CGCAAGTTTA CAAACAGAAA TTGAAAAGAA ACGTGCCGAA 
AATGATGATT TGCAGTCAAA ACTTTCTGTT GTAAGCTCGG AATACGAAAA TTTGCTGTTG 
ATTTCATCCC AAACAAACAA ATCTCTTGAA GACAAAACTA ACCAATTGAA GTATATTGAA 
AAGAACGTTC AAAAGTTATT GGATGAAAAG GATCAAAGGA ATGTCGAGTT GGAAGAACTA 
ACCTCCAAAT ATGGCAAACT TGGCGAAGAA AATGCCCAAA TTAAGGATGA GTTACTTGCC 
TTGAGGAAAA AGAGCAAGAA ACAGCATGAT CTTTGTGCAA ATTTTGTTGA TGACCTAAAG 
GAAAAGAGTG ATGCTCTCGA GCAATTGACT AACGAAAAAA ATGAGTTAAT TGTAAGCCTT 
GAGCAATCCA ATTCTAATAA TGAAGCACTC GTTGAAGAAA GGAGTGACTT AGCTAATCGT 
TTGTCAGACA TGAAAAAATC CTTGAGTGAT TCAGATAATG TTATATCTGT TATACGTTCT 
GATCTTGTAC GTGTAAATGA CGAGTTGGAT ACTCTTAAGA AGGATAAAGA TTCTTTGAGT 
ACTCAATATT CCGAAGTTTG TCAAGATCGT GATGATTTGC TAGACTCTCT GAAGGGTTGT 
GAAGAGAGTT TCAACAAGTA TGCTGTTTCT CTTCGTGAGT TATGTACCAA GTCTGAAATT 
GATGTTCCGG TATCTGAAAT TTTGGACGAT AATTTTGTCT TTAATGCTGG AAACTTTAGT 
GAATTAAGTC GCTTGACTGT TCTGTCTTTG GAAAACTATT TGGACGCTTT CAATCAAGTA 
AACTTCAAGA AAATGGAATT GGATAATCGA TTGACTACTA CCGACGCTGA ATTCACCAAG 
GTTGTTGCTG ATTTGGAGAA ATTGCAACAT GAACATGACG ATTGGTTAAT TCAAAGAGGA 
GATCTTGAAA AGGCACTGAA GGATAGTGAA AAGAATTTCT TACGGAAAGA AGCCGAGATG 
ACTGAAAATA TACATAGCTT AGAAGAAGGC AAAGAGGAAA CCAAAAAAGA AATAGCTGAA 
TTGTCCTCAA GGCTTGAAGA CAACCAACTT GCTACAAATA AGCTGAAAAA TCAACTTGAT 
CATTTAAATC AAGAAATTCG CCTGAAAGAA GACGTTTTAA AGGAGAAAGA AAGTTTAATC 
ATTTCATTGG AAGAAAGTTT AAGTAACCAA AGACAGAAAG AAAGCTCTTT GCTGGATGCT 
AAAAACGAGT TGGAACATAT GCTGGACGAT ACGTCGCGTA AGAATTCTTC ATTAATGGAA 
AAGATTGAAT CGATTAATTC TTCTTTGGAT GATAAGAGTT TTGAGCTCGC TTCGGCTGTT 
GAGAAGCTTG GTGCCCTGCA AAAGCTTCAC AGTGAGTCTC TTTCTTTAAT GGAAAACATC 
AAGTCACAGC TACAAGAGGC AAAGGAAAAG ATTCAAGTAG ATGAATCAAC TATTCAAGAG 
CTCGATCATG AAATTACTGC TTCTAAAAAT AATTATGAAG GAAAACTAAA TGATAAAGAT 
TCAATTATTA GAGATTTATC TGAGAATATT GAACAATTGA ATAACTTGCT TGCAGAGGAA 
AAGTCTGCTG TAAAAAGGCT GTCTACCGAA AAAGAATCAG AAATTTTGCA GTTCAACAGC 
CGCTTGGCAG ATTTGGAGTA CCATAAATCT CAAGTTGAGT CAGAATTAGG TCGTAGTAAA 
TTGAAGCTGG CTTCAACAAC GGAAGAATTG CAATTGGCTG AAAACGAACG ATTATCCTTA 
ACCACCCGAA TGTTAGATCT TCAAAACCAA GTCAAAGACC TTTCTAATAT AAAAGACAGT 
CTAAGCGAGG ATCTAAGAAC GTTACGATCG TTAGAAGATT CTGTAGCAAG TTTACAAAAA 
GAATGCAAGA TAAAATCTAA TACGGTTGAA TCATTGCAAG ATGTATTAAC AAGCGTACAA 
GCCCGTAACG CCGAATTGGA AGATGAAGTT TCTCGTTCAG TGGATAAAAT AAGAAGAAGG 
GATGATCGAT GTGAGCATTT AAGTGGAAAA CTTAAAAAAC TGCATTCTCA GTTAGAGGAA 
CAGCATGAAA CTTTCTTTAG AGCTGAACAG CAGCGTATGA CACAATTAGG GTTTCTCAAA 
GAAACAGTGA AAAAGCAAGA GAAGCTTTTG AAAAAACTAA ACTTAAGGCA AGAACAGTTA 
ATTCCTCGCT CTTCAATTTT AGTTTACGAA TCGTATATTC GTGACATTGA AAAAGAAATT 
ATAGTCTTAC AAGAACGTCT AAATGGCATT GAGCTTTCGC AACAACTTCC GAAGGGCTAC 
TTTGGGTATT TCTTCAAAAC TAATCGCGTC GAAATGGAAG TGTTGGATAG TTTCAAACAA 
CAAGTCGCTA AATTACAGTT CCTTGCGGGT GCGGAATTCA TTGTCAAATT TAAAGAGGAT 
CTTGAAAAAT GCGCTGCAGA AGAAAAGGAA AAACAAGCTA CATTTGACAA TTATTCTGAG 
AAGGTTGAAA ATCTTGGGAA ATCGATTGAA GCTTTGTATT TTGCACTAAA TCGAGAAATT 
AGTTTCCGAA AGTCTTTAGC GTTGTCTAAA TCAGCATATC ACAATCTTTT GGTTCGAGAT 
TCTCCCAAGT TTAACCCAGA TTCCCAAATT ACTTATAGTA TACCGGTTAC CAATACCAAG 
CAGTCATTGT TACGTTCTGC AATATTATGT GTCATATCAC TGCAACGTTT GAGATTATTA 
GGTCAAAGAC ATTCTTTTTG TGAAGAGGTT ATCGAGAACC TCTCTTGTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_593009.1
CDS	99..5972
Translation	MSNQSSSGSNTSDLDEESASSLVSSAASPFIDSDLETPRPNISRASTGQLAEDGDTSSQHEDSSEELKRQEVRGMRRHSDLSIDAKLGSSEGSTASSALPLTPRSPSNASWLLVRGGLLDSPILDINSVTQKSNLLNELKQVRSKLAALEHENGILSLQLSSSNKKDKNTSSVTTLTSEEDVSYFQKKLTNMESNFSAKQSEAYDLSRQLLTVTEKLDKKEKDYEKIKEDVSSIKASLAEEQASNKSLRGEQERLEKLLVSSNKTVSTLRQTENSLRAECKTLQEKLEKCAINEEDSKLLEELKHNVANYSDAIVHKDKLIEDLSTRISEFDNLKSERDTLSIKNEKLEKLLRNTIGSLKDSRTSNSQLEEEMVELKESNRTIHSQLTDAESKLSSFEQENKSLKGSIDEYQNNLSSKDKMVKQVSSQLEEARSSLAHATGKLAEINSERDFQNKKIKDFEKIEQDLRACLNSSSNELKEKSALIDKKDQELNNLREQIKEQKKVSESTQSSLQSLQRDILNEKKKHEVYESQLNELKGELQTEISNSEHLSSQLSTLAAEKEAAVATNNELSESKNSLQTLCNAFQEKLAKSVMQLKENEQNFSSLDTSFKKLNESHQELENNHQTITKQLKDTSSKLQQLQLERANFEQKESTLSDENNDLRTKLLKLEESNKSLIKKQEDVDSLEKNIQTLKEDLRKSEEALRFSKLEAKNLREVIDNLKGKHETLEAQRNDLHSSLSDAKNTNAILSSELTKSSEDVKRLTANVETLTQDSKAMKQSFTSLVNSYQSISNLYHELRDDHVNMQSQNNTLLESESKLKTDCENLTQQNMTLIDNVQKLMHKHVNQESKVSELKEVNGKLSLDLKNLRSSLNVAISDNDQILTQLAELSKNYDSLEQESAQLNSGLKSLEAEKQLLHTENEELHIRLDKLTGKLKIEESKSSDLGKKLTARQEEISNLKEENMSQSQAITSVKSKLDETLSKSSKLEADIEHLKNKVSEVEVERNALLASNERLMDDLKNNGENIASLQTEIEKKRAENDDLQSKLSVVSSEYENLLLISSQTNKSLEDKTNQLKYIEKNVQKLLDEKDQRNVELEELTSKYGKLGEENAQIKDELLALRKKSKKQHDLCANFVDDLKEKSDALEQLTNEKNELIVSLEQSNSNNEALVEERSDLANRLSDMKKSLSDSDNVISVIRSDLVRVNDELDTLKKDKDSLSTQYSEVCQDRDDLLDSLKGCEESFNKYAVSLRELCTKSEIDVPVSEILDDNFVFNAGNFSELSRLTVLSLENYLDAFNQVNFKKMELDNRLTTTDAEFTKVVADLEKLQHEHDDWLIQRGDLEKALKDSEKNFLRKEAEMTENIHSLEEGKEETKKEIAELSSRLEDNQLATNKLKNQLDHLNQEIRLKEDVLKEKESLIISLEESLSNQRQKESSLLDAKNELEHMLDDTSRKNSSLMEKIESINSSLDDKSFELASAVEKLGALQKLHSESLSLMENIKSQLQEAKEKIQVDESTIQELDHEITASKNNYEGKLNDKDSIIRDLSENIEQLNNLLAEEKSAVKRLSTEKESEILQFNSRLADLEYHKSQVESELGRSKLKLASTTEELQLAENERLSLTTRMLDLQNQVKDLSNIKDSLSEDLRTLRSLEDSVASLQKECKIKSNTVESLQDVLTSVQARNAELEDEVSRSVDKIRRRDDRCEHLSGKLKKLHSQLEEQHETFFRAEQQRMTQLGFLKETVKKQEKLLKKLNLRQEQLIPRSSILVYESYIRDIEKEIIVLQERLNGIELSQQLPKGYFGYFFKTNRVEMEVLDSFKQQVAKLQFLAGAEFIVKFKEDLEKCAAEEKEKQATFDNYSEKVENLGKSIEALYFALNREISFRKSLALSKSAYHNLLVRDSPKFNPDSQITYSIPVTNTKQSLLRSAILCVISLQRLRLLGQRHSFCEEVIENLSCV

Target ORF information:

RefSeq Version	NM_001018408.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe sporulation protein Spo15 (spo15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018408.2

ORF Insert Sequence:

ATGTCGAATC AGTCGTCTTC TGGTTCGAAT ACTTCTGATT TGGATGAAGA ATCAGCGAGT 
AGTCTTGTGA GCTCTGCTGC ATCACCATTT ATTGATTCTG ATCTCGAAAC ACCAAGACCC 
AATATTTCAA GAGCTTCAAC AGGACAACTT GCAGAAGACG GTGACACATC AAGTCAACAT 
GAAGATTCCT CAGAGGAGTT AAAGCGTCAA GAAGTAAGGG GAATGCGTAG GCACAGTGAC 
CTTTCAATTG ATGCGAAATT AGGCTCTTCT GAAGGCTCAA CTGCTTCTTC AGCATTACCT 
TTGACACCTC GTTCTCCTTC TAATGCCAGC TGGCTATTAG TCCGCGGTGG TTTACTTGAT 
AGTCCGATTC TCGACATTAA CTCAGTAACT CAAAAATCCA ACCTTTTAAA TGAGTTGAAG 
CAAGTGCGGT CCAAACTGGC TGCCCTTGAA CATGAAAACG GAATTCTCTC CTTGCAATTG 
TCATCTTCGA ATAAAAAGGA TAAGAATACC TCAAGTGTAA CCACGTTAAC ATCAGAGGAA 
GACGTGTCAT ATTTTCAAAA AAAGCTCACT AACATGGAGT CAAATTTTTC TGCTAAACAA 
TCTGAAGCGT ACGATTTATC GCGACAGTTA TTAACGGTTA CGGAAAAATT AGATAAAAAA 
GAAAAGGATT ACGAAAAAAT AAAAGAAGAT GTTTCTTCTA TCAAGGCCTC TTTAGCAGAA 
GAGCAAGCTT CTAACAAGTC CCTGCGTGGT GAGCAAGAGC GATTAGAAAA ACTTCTTGTA 
AGCTCCAATA AAACTGTTAG TACCTTAAGA CAAACAGAAA ACTCATTGCG CGCCGAATGT 
AAAACCTTGC AAGAAAAGTT GGAGAAATGC GCCATCAATG AGGAAGATTC CAAATTGCTG 
GAAGAACTTA AGCATAATGT CGCCAATTAT TCAGATGCCA TAGTACACAA GGATAAGTTA 
ATTGAAGATT TAAGTACCAG AATAAGCGAG TTTGATAATT TAAAATCTGA ACGGGACACG 
TTGTCAATAA AAAACGAGAA GCTAGAAAAG TTACTACGTA ATACGATTGG CAGCCTAAAA 
GACTCACGAA CCTCCAATTC TCAATTGGAA GAGGAAATGG TTGAGTTGAA AGAATCCAAT 
CGTACTATCC ATTCGCAATT GACTGATGCT GAATCTAAGC TTTCTTCTTT TGAACAGGAA 
AATAAATCAC TGAAAGGATC TATTGACGAG TACCAAAATA ATCTTTCTTC GAAGGATAAA 
ATGGTTAAAC AAGTTTCTTC CCAATTGGAA GAAGCTCGTT CTTCCCTTGC CCACGCGACT 
GGTAAATTAG CAGAGATAAA TTCCGAGCGA GATTTTCAAA ATAAAAAAAT AAAAGATTTT 
GAAAAGATAG AGCAGGACTT GCGTGCTTGT TTGAATTCCT CATCCAATGA ACTGAAGGAA 
AAATCAGCGT TGATTGACAA AAAAGATCAA GAGTTAAATA ATTTGAGGGA GCAGATTAAA 
GAACAGAAAA AAGTTTCTGA AAGTACTCAA TCAAGCCTTC AGTCTTTGCA ACGTGATATT 
TTAAATGAAA AGAAAAAACA TGAAGTTTAT GAAAGTCAAT TAAACGAACT GAAGGGTGAA 
TTACAAACGG AAATTTCAAA TTCTGAACAC CTATCCTCCC AACTCTCAAC TTTGGCGGCT 
GAGAAAGAGG CAGCTGTGGC TACCAATAAT GAACTTTCCG AAAGTAAAAA CTCACTTCAG 
ACATTGTGCA ATGCTTTTCA AGAAAAGCTT GCAAAATCGG TTATGCAATT GAAAGAGAAT 
GAGCAAAACT TTTCTTCCCT GGATACTTCA TTTAAAAAAC TCAATGAAAG TCACCAAGAA 
TTAGAAAATA ACCACCAGAC CATAACCAAG CAGCTGAAAG ATACTTCTTC AAAACTTCAA 
CAGCTACAAT TAGAGCGAGC CAATTTCGAA CAAAAAGAAT CAACCTTGAG CGACGAAAAT 
AATGATCTGC GAACTAAATT ATTAAAGCTC GAGGAGTCCA ACAAATCGTT AATTAAGAAA 
CAGGAAGACG TGGATTCTTT AGAGAAAAAT ATTCAAACAT TGAAGGAAGA CTTGCGGAAG 
TCGGAGGAAG CCCTCCGATT TTCCAAATTG GAGGCTAAAA ATTTGAGAGA AGTGATAGAC 
AATTTGAAAG GAAAGCATGA GACATTGGAA GCACAGAGAA ATGATTTGCA TTCTTCTCTT 
TCTGATGCTA AAAATACGAA TGCAATATTG TCGAGTGAAC TTACCAAATC ATCTGAAGAT 
GTCAAGAGGT TGACTGCCAA CGTTGAAACT CTTACACAAG ATAGCAAAGC TATGAAGCAA 
AGTTTTACAA GCCTTGTCAA TTCTTATCAG TCAATCTCAA ACTTGTACCA CGAACTTCGT 
GATGACCATG TGAATATGCA ATCTCAAAAT AATACATTGC TTGAATCTGA ATCAAAATTG 
AAGACAGACT GTGAAAATCT CACTCAACAG AACATGACAC TCATTGACAA TGTTCAGAAA 
CTCATGCATA AACATGTAAA TCAAGAATCC AAAGTTTCTG AACTAAAAGA GGTGAATGGA 
AAGTTATCAT TAGATTTGAA GAATCTAAGA TCCAGTTTAA ATGTTGCTAT TTCTGACAAT 
GATCAAATCC TTACCCAACT TGCTGAACTT AGTAAAAATT ACGATAGTTT GGAGCAAGAG 
TCTGCTCAGT TAAATTCCGG TCTGAAATCA TTGGAAGCTG AAAAGCAATT GCTACATACA 
GAAAATGAGG AGCTACATAT TCGTTTGGAT AAGTTAACTG GCAAGTTAAA AATTGAAGAA 
TCGAAATCCA GTGATCTTGG TAAAAAATTA ACTGCACGGC AAGAAGAAAT TTCAAACTTG 
AAAGAAGAAA ACATGTCGCA AAGTCAAGCC ATAACTTCTG TGAAATCTAA ACTTGATGAA 
ACTTTGTCAA AGTCTAGCAA GCTTGAAGCA GATATTGAAC ACCTGAAAAA TAAGGTTTCC 
GAGGTTGAAG TTGAAAGGAA TGCTCTTCTA GCTTCAAATG AACGTTTAAT GGACGATTTG 
AAAAATAATG GTGAAAATAT CGCAAGTTTA CAAACAGAAA TTGAAAAGAA ACGTGCCGAA 
AATGATGATT TGCAGTCAAA ACTTTCTGTT GTAAGCTCGG AATACGAAAA TTTGCTGTTG 
ATTTCATCCC AAACAAACAA ATCTCTTGAA GACAAAACTA ACCAATTGAA GTATATTGAA 
AAGAACGTTC AAAAGTTATT GGATGAAAAG GATCAAAGGA ATGTCGAGTT GGAAGAACTA 
ACCTCCAAAT ATGGCAAACT TGGCGAAGAA AATGCCCAAA TTAAGGATGA GTTACTTGCC 
TTGAGGAAAA AGAGCAAGAA ACAGCATGAT CTTTGTGCAA ATTTTGTTGA TGACCTAAAG 
GAAAAGAGTG ATGCTCTCGA GCAATTGACT AACGAAAAAA ATGAGTTAAT TGTAAGCCTT 
GAGCAATCCA ATTCTAATAA TGAAGCACTC GTTGAAGAAA GGAGTGACTT AGCTAATCGT 
TTGTCAGACA TGAAAAAATC CTTGAGTGAT TCAGATAATG TTATATCTGT TATACGTTCT 
GATCTTGTAC GTGTAAATGA CGAGTTGGAT ACTCTTAAGA AGGATAAAGA TTCTTTGAGT 
ACTCAATATT CCGAAGTTTG TCAAGATCGT GATGATTTGC TAGACTCTCT GAAGGGTTGT 
GAAGAGAGTT TCAACAAGTA TGCTGTTTCT CTTCGTGAGT TATGTACCAA GTCTGAAATT 
GATGTTCCGG TATCTGAAAT TTTGGACGAT AATTTTGTCT TTAATGCTGG AAACTTTAGT 
GAATTAAGTC GCTTGACTGT TCTGTCTTTG GAAAACTATT TGGACGCTTT CAATCAAGTA 
AACTTCAAGA AAATGGAATT GGATAATCGA TTGACTACTA CCGACGCTGA ATTCACCAAG 
GTTGTTGCTG ATTTGGAGAA ATTGCAACAT GAACATGACG ATTGGTTAAT TCAAAGAGGA 
GATCTTGAAA AGGCACTGAA GGATAGTGAA AAGAATTTCT TACGGAAAGA AGCCGAGATG 
ACTGAAAATA TACATAGCTT AGAAGAAGGC AAAGAGGAAA CCAAAAAAGA AATAGCTGAA 
TTGTCCTCAA GGCTTGAAGA CAACCAACTT GCTACAAATA AGCTGAAAAA TCAACTTGAT 
CATTTAAATC AAGAAATTCG CCTGAAAGAA GACGTTTTAA AGGAGAAAGA AAGTTTAATC 
ATTTCATTGG AAGAAAGTTT AAGTAACCAA AGACAGAAAG AAAGCTCTTT GCTGGATGCT 
AAAAACGAGT TGGAACATAT GCTGGACGAT ACGTCGCGTA AGAATTCTTC ATTAATGGAA 
AAGATTGAAT CGATTAATTC TTCTTTGGAT GATAAGAGTT TTGAGCTCGC TTCGGCTGTT 
GAGAAGCTTG GTGCCCTGCA AAAGCTTCAC AGTGAGTCTC TTTCTTTAAT GGAAAACATC 
AAGTCACAGC TACAAGAGGC AAAGGAAAAG ATTCAAGTAG ATGAATCAAC TATTCAAGAG 
CTCGATCATG AAATTACTGC TTCTAAAAAT AATTATGAAG GAAAACTAAA TGATAAAGAT 
TCAATTATTA GAGATTTATC TGAGAATATT GAACAATTGA ATAACTTGCT TGCAGAGGAA 
AAGTCTGCTG TAAAAAGGCT GTCTACCGAA AAAGAATCAG AAATTTTGCA GTTCAACAGC 
CGCTTGGCAG ATTTGGAGTA CCATAAATCT CAAGTTGAGT CAGAATTAGG TCGTAGTAAA 
TTGAAGCTGG CTTCAACAAC GGAAGAATTG CAATTGGCTG AAAACGAACG ATTATCCTTA 
ACCACCCGAA TGTTAGATCT TCAAAACCAA GTCAAAGACC TTTCTAATAT AAAAGACAGT 
CTAAGCGAGG ATCTAAGAAC GTTACGATCG TTAGAAGATT CTGTAGCAAG TTTACAAAAA 
GAATGCAAGA TAAAATCTAA TACGGTTGAA TCATTGCAAG ATGTATTAAC AAGCGTACAA 
GCCCGTAACG CCGAATTGGA AGATGAAGTT TCTCGTTCAG TGGATAAAAT AAGAAGAAGG 
GATGATCGAT GTGAGCATTT AAGTGGAAAA CTTAAAAAAC TGCATTCTCA GTTAGAGGAA 
CAGCATGAAA CTTTCTTTAG AGCTGAACAG CAGCGTATGA CACAATTAGG GTTTCTCAAA 
GAAACAGTGA AAAAGCAAGA GAAGCTTTTG AAAAAACTAA ACTTAAGGCA AGAACAGTTA 
ATTCCTCGCT CTTCAATTTT AGTTTACGAA TCGTATATTC GTGACATTGA AAAAGAAATT 
ATAGTCTTAC AAGAACGTCT AAATGGCATT GAGCTTTCGC AACAACTTCC GAAGGGCTAC 
TTTGGGTATT TCTTCAAAAC TAATCGCGTC GAAATGGAAG TGTTGGATAG TTTCAAACAA 
CAAGTCGCTA AATTACAGTT CCTTGCGGGT GCGGAATTCA TTGTCAAATT TAAAGAGGAT 
CTTGAAAAAT GCGCTGCAGA AGAAAAGGAA AAACAAGCTA CATTTGACAA TTATTCTGAG 
AAGGTTGAAA ATCTTGGGAA ATCGATTGAA GCTTTGTATT TTGCACTAAA TCGAGAAATT 
AGTTTCCGAA AGTCTTTAGC GTTGTCTAAA TCAGCATATC ACAATCTTTT GGTTCGAGAT 
TCTCCCAAGT TTAACCCAGA TTCCCAAATT ACTTATAGTA TACCGGTTAC CAATACCAAG 
CAGTCATTGT TACGTTCTGC AATATTATGT GTCATATCAC TGCAACGTTT GAGATTATTA 
GGTCAAAGAC ATTCTTTTTG TGAAGAGGTT ATCGAGAACC TCTCTTGTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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spo15 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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