smc5 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	smc5
Entrez Gene ID	2541587
Full Name	Smc5-6 complex SMC subunit Smc5
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001020338.2	NP_594907.2	Smc5-6 complex SMC subunit Smc5

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Smc5	NP_001099827.1
Danio rerio (zebrafish)	smc5	NP_001180470.1
Drosophila melanogaster (fruit fly)	Smc5	NP_996141.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011623	XP_320881.2
Homo sapiens (human)	SMC5	NP_055925.2
Bos taurus (cattle)	SMC5	XP_002689686.1
Arabidopsis thaliana (thale cress)	SMC5	NP_197096.1
Xenopus tropicalis (tropical clawed frog)	smc5	XP_004910829.1
Pan troglodytes (chimpanzee)	SMC5	XP_520066.3
Mus musculus (house mouse)	Smc5	NP_001239613.1
Kluyveromyces lactis	KLLA0F07997g	XP_455442.1
	SMC5	XP_001090492.2
Canis lupus familiaris (dog)	SMC5	XP_533529.3
Saccharomyces cerevisiae (baker's yeast)	SMC5	NP_014608.1
Schizosaccharomyces pombe (fission yeast)	smc5	NP_594907.2
Neurospora crassa	NCU09065	XP_963793.1
Gallus gallus (chicken)	SMC5	NP_001034424.1
Eremothecium gossypii	AGOS_AEL337C	NP_984523.2
Magnaporthe oryzae (rice blast fungus)	MGG_12590	XP_003720930.1
Oryza sativa (rice)	Os05g0596600	NP_001056521.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The function of the SMC5/6 complex and its mammalian homologs in the cell cycle has not been clarified yet.
Title: The (elusive) role of the SMC5/6 complex.

Smc6 contributes to DNA repair in vivo by targeting the Smc5-6 complex to single-stranded DNA substrates created during the processes of homologous recombination and/or DNA replication.
Title: DNA-binding properties of Smc6, a core component of the Smc5-6 DNA repair complex.

Smc5 is a required for homologous recombination in S. pombe.
Title: Nse1-dependent recruitment of Smc5/6 to lesion-containing loci contributes to the repair defects of mutant complexes.

In an examination of the evolutionary conservation of the Nse3-Nse4 interactions, we find that several MAGE proteins can interact with at least one of the NSE4/EID proteins.
Title: Interactions between the Nse3 and Nse4 components of the SMC5-6 complex identify evolutionarily conserved interactions between MAGE and EID Families.

Data show that defective cohesin removal is a major determinant of the mitotic lethality of Smc5-Smc6 mutants.
Title: Smc5-Smc6-dependent removal of cohesin from mitotic chromosomes.

The following smc5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smc5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102118	NM_001020338.2 Latest version!	Schizosaccharomyces pombe Smc5-6 complex SMC subunit Smc5 (smc5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smc5 ( NM_001020338.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594907.2 Schizosaccharomyces pombe Smc5-6 complex SMC subunit Smc5 (smc5), mRNA.

Price & Availability↑

CloneID	OSc102118
Clone ID Related Accession (Same CDS sequence)	NM_001020338.2
Accession Version	NM_001020338.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	Smc5-6 complex SMC subunit Smc5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001020338.1.

ATGGATGGCC TTAGGCCTTC GAAACGAAGA AAAAGTAATC CTTTGTATTC AGATTACGCG 
TTAGGAAGTA TTGTACGAAT TAAATTAGTG AACTTTGTTA CATACGATTA TTGTGAACTG 
TTCCCTGGTC CATATCTAAA TTTGATTATC GGTCCAAATG GGACAGGTAA GAGCACAATT 
GTTTCAGCAA TATGCATTGG GCTGGGATGG CCCCCAAAAC TACTTGGGAG AGCTAAAGAG 
GCCCGTGAAT TTATAAAGTA TGGCAAAAAC ACTGCAACCA TAGAAATTGA AATGAAGTAT 
CGTGACGATG AAACGGTTAC TATCACGAGA CAAATAAGCC AGGATAAGAG TAGTTCTTTC 
TCTATTAATA GGGAAGCATG TGCCACTTCT TCAATAACGT CTCTAATGGA TACTTTCAAT 
GTACAATTGA ATAATCTTTG TCATTTTTTA CCACAAGACC GTGTTGCAGA ATTCGCTCAG 
CTTGATCCTT ATTCTAGACT GATGGAAACT GAGCGTGCAA TTGACCATGA AGGGTTACTT 
CCCGCACATG AGAAGCTTAT TGATTTACGA AAACGAGAAA GGGAAATTTT ACAAAATAAA 
AACCAAGGAC AATCAACATT AAATTCATTA AAAGATAGAC AACAGGCACT AGAGAAGGAA 
GTCAATATAT TTAAAGAACG GGAAAAAATC AAATCATACA TCGAAATGCT AGGGTTAGCT 
AAAATGTTAG TTATATACAG AGAGAAAACC AATGTCTTTA ACCAACTTCG TGCAGACAAA 
AAAAAATTAA AAAAGGATTT GAAAGACCTA GTGGAAGAAT TTCAACCTAT TTTGGATAAA 
GGTGAAGAGC TGCGTTCGGA TTTAAAACTA AAAGATGATA CATTTAACGA TTACTCCTCC 
GCTTCGATGG AACTTAATAC CTCAAATTTA AGAGCCAGAG CTTCTTTTTC GAATTTCATG 
GAGAACGAGA AAAAGCTTTA TGAGAAGGTT AATACCAATC GAACACTTTT ACGGAATGCA 
AATCTTACAT TAAATGAAGC ACAACAATCG GTGAAGTCAT TAACAGAAAG ACAAGGCCCA 
AGACCTTCGG ATAACGGTGT TCAAGATCTA CAAGAAAAGA TGCAAGAAGT TAATGCGGAA 
AAACTTCAAC ATGAAAACGA GAAACTTGAG AGCTCTCATG AACTAGGTAG TATCAGAACA 
TTGAAAGCAC AAAAGCTTAT TGACCTTGAT AACATTAAAA GAGAGTTGTC CTACTACAAC 
GATGCTACGA AAAGGAAACT AGACTTTATG TCTTCTGCTC CAGGCTGGGA AGATGCTTAT 
CAGACGTATC AGTTATTGAA GGAATACGAA TCTGCGTTTG AAGCACCCGC ATACGGCCCA 
ATTTATATGA ATTTGAAATG TAAGGAAAAA GGGTTTGCAG CTTTGATAGA AGGGTTTTTT 
CGCACGGATA CGTTTCGAAC ATTTATAATG TCAAATTATA ATGATTATCT AAAGTTAATG 
GATCTTATCA CTTCAAAAAC TAAGTACACT CCTACTATAA GAGAATTCTC CAGTGAACGA 
AAAAAGAAGA TAGAAGATTT TGAACCTCCA TGCTCTCGTG AGAAGCTTCA GTCTTTCGGG 
TTTGACGGAT ATGTGATCGA TTTTTTAGAG GGGCCAGAAG TGGTTTTGGT TGCTTTGTGC 
CATATGTTAA AAATTCATCA AATTCCGATT GCAAAAAGGG AACTACCTCC CGCATCAGTG 
AATGCGTTAA ACAATTTTCG TCTGGCTAAC GGGGATCCAG TTCTTAAAAC TTACTTGGCG 
GGCTCATCTA TCCACTTGGT TTTTCGTTCA GCTTACGGTG ATAGAGAAAT TACTAGGCGA 
ACAGACCCAT TACCTTCTCG CAGTATATAT TTTTCCGAGA ACGTTGAGAT GGATTTGGTA 
AAGCGAAAGG AGGAGCAACT AAATGCTCAA CTCTCTCAAT TGGAAAATTT ACAGAATGAG 
GAAAGAAAGT TACAAGAAAA GGTAAATGAG CACGAATCGC TGCTTTCTCG TACAAACGAT 
ATATTATCTA CTCTACGAAA AGAGCGTGAC GAAAAATTAA TTCCTATTCA CGAATGGCAA 
CAATTACAAG AAAGAATAGA ACACCAAACT CTTCTACTAA GACAAAGAGA GAAAGTTCCT 
GAGCAATTTG CAGCTGAAAT TGAAAAAAAT GAAGATATCA GGAAAGAGAA CTTCGAAGCT 
CTTATGAATT CGGTGTTAAA AGTTAAGGAG AATTCAATTA AAGCTACGAA TAATTTCGAG 
AAGATGTTGG GAAGTAGACT TAATGTCATA GAAGCGAAAT ACAAACTTGA AAAACACGAG 
ATGGATGCCA ACCAAGTCAA CGCTCGATTG ACAGAAGTAC AAGATCGTCT TAAAGATATT 
ACCGATAAGT TAGCCTCAGC AAGGGAGGAT GCTATGTCCT TGTACGGTTC AGTGGTTGAT 
TCATTGCAGA CACAATCATC TGACAGACAA ACGGCAATAA CTGAATTAAA TGAAGAATTT 
GCTACTAGTT CGGAAGTGGA TAACAAAATT AGCATCGAAG AAACGAAACT GAAGTTTATG 
AATGTTAATA GTTACGTTAT GGAACAATAT GATGCGCGTA AAAAAGAAAT TGAGGAACTA 
GAATCGAAAA TGTCAGACTT TGATCAATCC GTTGAAGAGC TCCAAGACGA GATGAACTCA 
ATCAAAGAGG ATTGGGTTAG TAAGTTAGAG GAGAATGTTC AATGTATTAG CGATAGATTT 
TCTAAAGGAA TGAGTGGAAT GGGATATGCT GGAGAAGTTC GACTAGGGAA ATCCGACGAT 
TATGATAAAT GGTATATCGA TATTTTAGTA CAATTTCGAG AGGAAGAAGG ACTTCAAAAA 
CTTACGGGTC AACGACAATC TGGTGGAGAA CGTTCTGTAT CTACTATAAT GTATTTATTA 
TCTTTACAAG GTTTGGCAAT TGCTCCGTTT CGAATAGTTG ATGAAATAAA TCAAGGAATG 
GATCCTCGAA ATGAACGAGT TGTGCATAGG CATATCGTCA ATTCAGTGTG TGACAACGCT 
GTTTCACAAT ACTTCTTAGT CACTCCAAAG TTATTACCAG ATTTGACATA CCATCGAAAT 
TTGAAAGTGC TATGTATTTG TAATGGTGCT TGGTTACCAG CAACTTTTAG AACATCCCTT 
TCTACCTATT TTGAAAAGTT AAAGAAGTCC GCACTCATTT CTTCGTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594907.2
CDS	507..3737
Translation	MDGLRPSKRRKSNPLYSDYALGSIVRIKLVNFVTYDYCELFPGPYLNLIIGPNGTGKSTIVSAICIGLGWPPKLLGRAKEAREFIKYGKNTATIEIEMKYRDDETVTITRQISQDKSSSFSINREACATSSITSLMDTFNVQLNNLCHFLPQDRVAEFAQLDPYSRLMETERAIDHEGLLPAHEKLIDLRKREREILQNKNQGQSTLNSLKDRQQALEKEVNIFKEREKIKSYIEMLGLAKMLVIYREKTNVFNQLRADKKKLKKDLKDLVEEFQPILDKGEELRSDLKLKDDTFNDYSSASMELNTSNLRARASFSNFMENEKKLYEKVNTNRTLLRNANLTLNEAQQSVKSLTERQGPRPSDNGVQDLQEKMQEVNAEKLQHENEKLESSHELGSIRTLKAQKLIDLDNIKRELSYYNDATKRKLDFMSSAPGWEDAYQTYQLLKEYESAFEAPAYGPIYMNLKCKEKGFAALIEGFFRTDTFRTFIMSNYNDYLKLMDLITSKTKYTPTIREFSSERKKKIEDFEPPCSREKLQSFGFDGYVIDFLEGPEVVLVALCHMLKIHQIPIAKRELPPASVNALNNFRLANGDPVLKTYLAGSSIHLVFRSAYGDREITRRTDPLPSRSIYFSENVEMDLVKRKEEQLNAQLSQLENLQNEERKLQEKVNEHESLLSRTNDILSTLRKERDEKLIPIHEWQQLQERIEHQTLLLRQREKVPEQFAAEIEKNEDIRKENFEALMNSVLKVKENSIKATNNFEKMLGSRLNVIEAKYKLEKHEMDANQVNARLTEVQDRLKDITDKLASAREDAMSLYGSVVDSLQTQSSDRQTAITELNEEFATSSEVDNKISIEETKLKFMNVNSYVMEQYDARKKEIEELESKMSDFDQSVEELQDEMNSIKEDWVSKLEENVQCISDRFSKGMSGMGYAGEVRLGKSDDYDKWYIDILVQFREEEGLQKLTGQRQSGGERSVSTIMYLLSLQGLAIAPFRIVDEINQGMDPRNERVVHRHIVNSVCDNAVSQYFLVTPKLLPDLTYHRNLKVLCICNGAWLPATFRTSLSTYFEKLKKSALISSS

Target ORF information:

RefSeq Version	NM_001020338.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe Smc5-6 complex SMC subunit Smc5 (smc5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020338.2

ORF Insert Sequence:

ATGGATGGCC TTAGGCCTTC GAAACGAAGA AAAAGTAATC CTTTGTATTC AGATTACGCG 
TTAGGAAGTA TTGTACGAAT TAAATTAGTG AACTTTGTTA CATACGATTA TTGTGAACTG 
TTCCCTGGTC CATATCTAAA TTTGATTATC GGTCCAAATG GGACAGGTAA GAGCACAATT 
GTTTCAGCAA TATGCATTGG GCTGGGATGG CCCCCAAAAC TACTTGGGAG AGCTAAAGAG 
GCCCGTGAAT TTATAAAGTA TGGCAAAAAC ACTGCAACCA TAGAAATTGA AATGAAGTAT 
CGTGACGATG AAACGGTTAC TATCACGAGA CAAATAAGCC AGGATAAGAG TAGTTCTTTC 
TCTATTAATA GGGAAGCATG TGCCACTTCT TCAATAACGT CTCTAATGGA TACTTTCAAT 
GTACAATTGA ATAATCTTTG TCATTTTTTA CCACAAGACC GTGTTGCAGA ATTCGCTCAG 
CTTGATCCTT ATTCTAGACT GATGGAAACT GAGCGTGCAA TTGACCATGA AGGGTTACTT 
CCCGCACATG AGAAGCTTAT TGATTTACGA AAACGAGAAA GGGAAATTTT ACAAAATAAA 
AACCAAGGAC AATCAACATT AAATTCATTA AAAGATAGAC AACAGGCACT AGAGAAGGAA 
GTCAATATAT TTAAAGAACG GGAAAAAATC AAATCATACA TCGAAATGCT AGGGTTAGCT 
AAAATGTTAG TTATATACAG AGAGAAAACC AATGTCTTTA ACCAACTTCG TGCAGACAAA 
AAAAAATTAA AAAAGGATTT GAAAGACCTA GTGGAAGAAT TTCAACCTAT TTTGGATAAA 
GGTGAAGAGC TGCGTTCGGA TTTAAAACTA AAAGATGATA CATTTAACGA TTACTCCTCC 
GCTTCGATGG AACTTAATAC CTCAAATTTA AGAGCCAGAG CTTCTTTTTC GAATTTCATG 
GAGAACGAGA AAAAGCTTTA TGAGAAGGTT AATACCAATC GAACACTTTT ACGGAATGCA 
AATCTTACAT TAAATGAAGC ACAACAATCG GTGAAGTCAT TAACAGAAAG ACAAGGCCCA 
AGACCTTCGG ATAACGGTGT TCAAGATCTA CAAGAAAAGA TGCAAGAAGT TAATGCGGAA 
AAACTTCAAC ATGAAAACGA GAAACTTGAG AGCTCTCATG AACTAGGTAG TATCAGAACA 
TTGAAAGCAC AAAAGCTTAT TGACCTTGAT AACATTAAAA GAGAGTTGTC CTACTACAAC 
GATGCTACGA AAAGGAAACT AGACTTTATG TCTTCTGCTC CAGGCTGGGA AGATGCTTAT 
CAGACGTATC AGTTATTGAA GGAATACGAA TCTGCGTTTG AAGCACCCGC ATACGGCCCA 
ATTTATATGA ATTTGAAATG TAAGGAAAAA GGGTTTGCAG CTTTGATAGA AGGGTTTTTT 
CGCACGGATA CGTTTCGAAC ATTTATAATG TCAAATTATA ATGATTATCT AAAGTTAATG 
GATCTTATCA CTTCAAAAAC TAAGTACACT CCTACTATAA GAGAATTCTC CAGTGAACGA 
AAAAAGAAGA TAGAAGATTT TGAACCTCCA TGCTCTCGTG AGAAGCTTCA GTCTTTCGGG 
TTTGACGGAT ATGTGATCGA TTTTTTAGAG GGGCCAGAAG TGGTTTTGGT TGCTTTGTGC 
CATATGTTAA AAATTCATCA AATTCCGATT GCAAAAAGGG AACTACCTCC CGCATCAGTG 
AATGCGTTAA ACAATTTTCG TCTGGCTAAC GGGGATCCAG TTCTTAAAAC TTACTTGGCG 
GGCTCATCTA TCCACTTGGT TTTTCGTTCA GCTTACGGTG ATAGAGAAAT TACTAGGCGA 
ACAGACCCAT TACCTTCTCG CAGTATATAT TTTTCCGAGA ACGTTGAGAT GGATTTGGTA 
AAGCGAAAGG AGGAGCAACT AAATGCTCAA CTCTCTCAAT TGGAAAATTT ACAGAATGAG 
GAAAGAAAGT TACAAGAAAA GGTAAATGAG CACGAATCGC TGCTTTCTCG TACAAACGAT 
ATATTATCTA CTCTACGAAA AGAGCGTGAC GAAAAATTAA TTCCTATTCA CGAATGGCAA 
CAATTACAAG AAAGAATAGA ACACCAAACT CTTCTACTAA GACAAAGAGA GAAAGTTCCT 
GAGCAATTTG CAGCTGAAAT TGAAAAAAAT GAAGATATCA GGAAAGAGAA CTTCGAAGCT 
CTTATGAATT CGGTGTTAAA AGTTAAGGAG AATTCAATTA AAGCTACGAA TAATTTCGAG 
AAGATGTTGG GAAGTAGACT TAATGTCATA GAAGCGAAAT ACAAACTTGA AAAACACGAG 
ATGGATGCCA ACCAAGTCAA CGCTCGATTG ACAGAAGTAC AAGATCGTCT TAAAGATATT 
ACCGATAAGT TAGCCTCAGC AAGGGAGGAT GCTATGTCCT TGTACGGTTC AGTGGTTGAT 
TCATTGCAGA CACAATCATC TGACAGACAA ACGGCAATAA CTGAATTAAA TGAAGAATTT 
GCTACTAGTT CGGAAGTGGA TAACAAAATT AGCATCGAAG AAACGAAACT GAAGTTTATG 
AATGTTAATA GTTACGTTAT GGAACAATAT GATGCGCGTA AAAAAGAAAT TGAGGAACTA 
GAATCGAAAA TGTCAGACTT TGATCAATCC GTTGAAGAGC TCCAAGACGA GATGAACTCA 
ATCAAAGAGG ATTGGGTTAG TAAGTTAGAG GAGAATGTTC AATGTATTAG CGATAGATTT 
TCTAAAGGAA TGAGTGGAAT GGGATATGCT GGAGAAGTTC GACTAGGGAA ATCCGACGAT 
TATGATAAAT GGTATATCGA TATTTTAGTA CAATTTCGAG AGGAAGAAGG ACTTCAAAAA 
CTTACGGGTC AACGACAATC TGGTGGAGAA CGTTCTGTAT CTACTATAAT GTATTTATTA 
TCTTTACAAG GTTTGGCAAT TGCTCCGTTT CGAATAGTTG ATGAAATAAA TCAAGGAATG 
GATCCTCGAA ATGAACGAGT TGTGCATAGG CATATCGTCA ATTCAGTGTG TGACAACGCT 
GTTTCACAAT ACTTCTTAGT CACTCCAAAG TTATTACCAG ATTTGACATA CCATCGAAAT 
TTGAAAGTGC TATGTATTTG TAATGGTGCT TGGTTACCAG CAACTTTTAG AACATCCCTT 
TCTACCTATT TTGAAAAGTT AAAGAAGTCC GCACTCATTT CTTCGTCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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smc5 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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