ORF » Species Summary » Schizosaccharomyces pombe » SPAC25B8.05 cDNA ORF clone

SPAC25B8.05 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following SPAC25B8.05 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAC25B8.05 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc103251 NM_001019894.2
Latest version!		 Schizosaccharomyces pombe putative tRNA-pseudouridine synthase (SPAC25B8.05), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    SPAC25B8.05 ( NM_001019894.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594465.1 Schizosaccharomyces pombe putative tRNA-pseudouridine synthase (SPAC25B8.05), mRNA.
    Price & Availability↑
    CloneID OSc103251
    Clone ID Related Accession (Same CDS sequence) NM_001019894.2
    Accession Version NM_001019894.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative tRNA-pseudouridine synthase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019894.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCCCGCCT CCAAATATGC TGGATGGTCT CGAGACCAGC TAATATCCAG AATTTTAGAA 
    TTGGACGGGA AATTGCAAAA AAATACAAAT TCACTTCCCA ATAAAGAAAT TGAGGGGAAA 
    AATGAAGTAA TTAAAAATAA AAACAAATTG GATAATGGAA ATGAAATAGA ACCCCAGCAT 
    CTGAGCCAAG AACTAGTTTT GCAACATGCG CTTCAGGACG CAAGTAGTCT TCCAAAACTG 
    AAACCTGTCA GACCGTTTCA ACTAGAAAAA ACAGTTTTTG TTGCTTTTAA ATTTGCCTAT 
    TGTGGTTGGA ATTACAATGG TCTTGCCTAC CAGTTAGAAC CGACTCCTTT GCCCACTGTT 
    GAAGGAAAGG TGTTTGAAGC GCTGTTGAGA GCTCATCTCA TCACTGATCC TTCATCATGT 
    TCCTTTAGTC GATGTGGACG TACTGATAAG GGAGTTTCAG CCATGGGTCA GGTTATATCA 
    TTAAATGTAC GACATTCAGA AAGAAGACCT ATCATATATT GTGATGTATT GAATCGCCTG 
    TTGCCCGCAG ATATACGTAT TACTGGCTAT GCATTTCCAC CAGAAGGCTT TGATGCTCGA 
    TTTTCCTGTA AACAGCGTCA TTATAAATAC TTGTTTACTA AGGATTATCC CGGTGGTTCT 
    CTTGACATTG ATCGAATGAA TGAAGCCGCA GCACTTTATC TAGGTGAGCA TGACTTTCGA 
    AATTTTTGCA AAATAGATGC TTCTAAACAA ATTACAAATT ATCACCGTCG AATTCTCAGT 
    TCAAAGGTTA TTTGCATTGA TCCCAGTACC GGTCTTTACG CTTTTGACCT ACAAGGAACT 
    GCATTTTTAT GGCATCAGGT ACGATGTATG ATGGCCATTC TATTTTTAAT TGGTCAAAAG 
    CTTGAGCCGG CCTCTCTCAT CAATGATCTT TTGGATATTA AAAAGGTCCC TACTAAGCCA 
    ATTTATGATA TGGCAAGTGA GTATCCTTTG ATTTTGTACG ACTGTGACTT TGATAATATT 
    GAATGGACAC TACCTACGGA TAGTCCTACG ACTGCTCCTC GTATCGCTAA ACATACATAT 
    GAAACCATAT ATCATCAGTG GCATAGTCTA CGTATTCGAG AGCAGATAGC TTCGTTTATG 
    CTTGACATAG CAGATCATAA TGTAAAAAGA TATGGCAAAT CGGAAGAGTC AAGCAGTACT 
    ATGAACGTTG GTGAAGGATT GCTGAAGCGA ACAAAAAAGT ACATCCCAAT TCTTTCTAGA 
    CAACGTCAGG AATCAGTGGA GGTCGTCAAT GAAAGATATA AACGTAAAAA AGGTTTAAAA 
    GACAGTGACG TGAAGAGTGA AAATATTAAA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_594465.1
    CDS524..1876
    Translation

    Target ORF information:

    RefSeq Version NM_001019894.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative tRNA-pseudouridine synthase (SPAC25B8.05), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001019894.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCCCGCCT CCAAATATGC TGGATGGTCT CGAGACCAGC TAATATCCAG AATTTTAGAA 
    TTGGACGGGA AATTGCAAAA AAATACAAAT TCACTTCCCA ATAAAGAAAT TGAGGGGAAA 
    AATGAAGTAA TTAAAAATAA AAACAAATTG GATAATGGAA ATGAAATAGA ACCCCAGCAT 
    CTGAGCCAAG AACTAGTTTT GCAACATGCG CTTCAGGACG CAAGTAGTCT TCCAAAACTG 
    AAACCTGTCA GACCGTTTCA ACTAGAAAAA ACAGTTTTTG TTGCTTTTAA ATTTGCCTAT 
    TGTGGTTGGA ATTACAATGG TCTTGCCTAC CAGTTAGAAC CGACTCCTTT GCCCACTGTT 
    GAAGGAAAGG TGTTTGAAGC GCTGTTGAGA GCTCATCTCA TCACTGATCC TTCATCATGT 
    TCCTTTAGTC GATGTGGACG TACTGATAAG GGAGTTTCAG CCATGGGTCA GGTTATATCA 
    TTAAATGTAC GACATTCAGA AAGAAGACCT ATCATATATT GTGATGTATT GAATCGCCTG 
    TTGCCCGCAG ATATACGTAT TACTGGCTAT GCATTTCCAC CAGAAGGCTT TGATGCTCGA 
    TTTTCCTGTA AACAGCGTCA TTATAAATAC TTGTTTACTA AGGATTATCC CGGTGGTTCT 
    CTTGACATTG ATCGAATGAA TGAAGCCGCA GCACTTTATC TAGGTGAGCA TGACTTTCGA 
    AATTTTTGCA AAATAGATGC TTCTAAACAA ATTACAAATT ATCACCGTCG AATTCTCAGT 
    TCAAAGGTTA TTTGCATTGA TCCCAGTACC GGTCTTTACG CTTTTGACCT ACAAGGAACT 
    GCATTTTTAT GGCATCAGGT ACGATGTATG ATGGCCATTC TATTTTTAAT TGGTCAAAAG 
    CTTGAGCCGG CCTCTCTCAT CAATGATCTT TTGGATATTA AAAAGGTCCC TACTAAGCCA 
    ATTTATGATA TGGCAAGTGA GTATCCTTTG ATTTTGTACG ACTGTGACTT TGATAATATT 
    GAATGGACAC TACCTACGGA TAGTCCTACG ACTGCTCCTC GTATCGCTAA ACATACATAT 
    GAAACCATAT ATCATCAGTG GCATAGTCTA CGTATTCGAG AGCAGATAGC TTCGTTTATG 
    CTTGACATAG CAGATCATAA TGTAAAAAGA TATGGCAAAT CGGAAGAGTC AAGCAGTACT 
    ATGAACGTTG GTGAAGGATT GCTGAAGCGA ACAAAAAAGT ACATCCCAAT TCTTTCTAGA 
    CAACGTCAGG AATCAGTGGA GGTCGTCAAT GAAAGATATA AACGTAAAAA AGGTTTAAAA 
    GACAGTGACG TGAAGAGTGA AAATATTAAA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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