irs1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following irs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the irs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100438 NM_001021464.2
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Schizosaccharomyces pombe putative isoleucine--tRNA(Ile) ligase Irs1 (irs1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc100438
    Clone ID Related Accession (Same CDS sequence) NM_001021464.2
    Accession Version NM_001021464.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3195bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative isoleucine--tRNA(Ile) ligase Irs1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021464.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCGTTTA ACGTTCCTAA AGAAGAGGAA AAAATCGTTG AGTTTTGGCG CGAAATCGAT 
    GCGTTCCATA CTCAGTTAAA ACTTTCTCAA GGTCGTCCTA CTTATACATT TTTCGATGGA
    CCTCCATTCG CTACCGGTCG TCCTCATCAT GGTCATCTTT TGGCTTCTAC CATTAAGGAT
    AGTGTCACCC GTTATGCTTG CTTGAAGGGT TATCACGTCG AGCGTAGATT CGGTTGGGAT
    ACACATGGAC TTCCTGTTGA GCATGAAATC GACAAAAAGT TGGGTATTAC TGGAAGTGAT
    GATGTTATGG CTATGGGTAT TGACAAATAC AATGCCGAAT GTCGTAAAAT TGTCATGACT
    TATGCATCCG AATGGCGTGC AACAGTTGAA AGACTGGGCC GTTGGATTGA TTTTGATAAC
    GATTATAAAA CGTTGTATCC TTCTTTCATG GAAAGTGTTT GGTGGGTTTT CAAAGAGTTG
    CATACAAAAG GAAAAGTTTA CCGCGGATAT CGTGTTATGC CTTACTCGAC TGCATGTACA
    ACACCTTTGA GTAACTTTGA AGCTCAACAA AACTATAAGG AAGTCCCAGA TCCGGCTATT
    GTTGTTGCCT TTCAATCCAT TAGTGATCCT GAAGTTTCTT TCTTAGCTTG GACTACTACT
    CCATGGACCT TGCCTTCTAA CCTTGCTTTG GCTGTTCACC CTGACTTACA GTACATAAAA
    ATTTTGGACA AGGATTCTAA TAAGAAATAT ATCCTTATGG AGAGCTGCCT TGGCATCCTT
    TACAAGAACC CAAAAAAGGC GAACTTTGAA ATTTTGGAAC GTTTCCAAGG AAAGGCCTTG
    GATGGTCAAA AGTATGAACC TCTCTTCCCT TATTTCAAGA GCACTTTTGG CGAAAGAGCA
    TTTAAATTAT ATTCTGCTGA CTACGTTGAA GAAGGCAGTG GTACTGGTAT CGTCCATCAA
    GCTCCTGCTT TTGGTGAAGC AGATTATGAC GCTGCTTGGG CTGCTGGCAT CATCGATGCT
    GATCACCAAC CTCCTTGCCC TGTTGATGAG CAAGGTTTGC TTACTTCGGA AATCACAGAC
    TTCGCTGGCC AATATGTTAA AGACGCAGAT AAGGAAATTA TCCGCTCCTT GAAAGCATCT
    GGCCATTTGG TTAAGCATAG TCAAATTTTC CATAGTTATC CATTTTGCTG GCGTTCCGAT
    ACACCTCTAA TTTATCGTGC CGTTCCCAGT TGGTTTGTTC GCGTGAAGGA GATCACTAAT
    GAGATGGTTG AAAATGTCAT GAGCACTCAC TGGGTCCCTC AAAACATTCG TGATAAACGT
    TTTGCGAATT GGTTGAAGAA TGCTCGTGAC TGGAACATTT CCCGTAACAG ATACTGGGGT
    ACCCCTATCC CACTTTGGGT TAGTGATGAC TACGAAGAAG TCGTTTGCAT TGGTTCCATT
    AAGGAGCTTG AAGAACTTTC CGGCGTTTCT AATATTACAG ACATTCATCG TGACAGTATT
    GATCATATCA CTATTCCTTC TAAAAAAGGT AAAGGAACAT TGCATCGTGT CAGTGAAGTA
    TTTGACTGTT GGTTTGAATC TGGCAGCATG CCTTATGCTT CTCGTCATTA CCCTTTCGAA
    AGAATTGAGG AATTCAAGCA TGGCTTCCCT GCTGACTTTA TTTCCGAGGG TGTTGATCAA
    ACCCGTGGAT GGTTTTATAC TTTAACTGTT TTGGGAACTT TGTTATTCGA CAAAGCACCT
    TATAAAAATG TAATTGTTAG TGGTCTGGTA ATGGCTGAGG ATGGTAAAAA AATGTCCAAA
    CGTCTTAAAA ATTACCCTGA ACCAAATCTC ATCATTGAAA AATATGGTTC TGATGCCCTT
    CGTTTGTATC TCATCAACTC TCCAGTTGTT CGTGCTGAGA TTCTTAAATT TAAGGAAGAT
    GGTGTAAGAG AAGTTGTTAC TAGAGTTTTG ATTCCTTGGT GGAACAGTTA TAAGTTTTTT
    GAGGCGCAAG CTGCTCTTTA CAAGAAGGTT ACTGGTAAAG ACTTTGTTTT CGATGATGCT
    GCCACTTTGA GTTCCAATGT AATGGATCGC TGGATTTTAG CTCGTTGCCA GAGCTTGATT
    GGATTTGTTG ATGAAGAAAT GAAACAATAC CGTTTGTACA CCGTGGTCCC TCAACTTCTC
    GGTTTAATTG AGGAGATGAC TAACTGGTAT ATCCGGTTTA ACAGACGTCG TTTGAAGGGT
    GAAGATGGTG AAATTGAAAC TATTAATGCT TTGAACGTTT TGTTTGAAGT CTTGTTCACG
    TTAGTTCGTA TTATGGGCCC ATTCACCCCT TTCATTACTG AAAACATTTA TCAACACCTT
    AGAAACTACA TGCCTATTGA CAAAAATGAA ATTTCACTTC GTTCAGTTCA TTTCCTTCCT
    TTCCCTACTT ACAAGTCTGA GCTTGATGAT GAAACTGTTT TACGCCGTGT TAAGCGTATG
    CAAACTATTA TCGAATTGGC GCGTTATGTT CGTGAGCAGA ATAATATTTC TTTGAAGACT
    CCCTTAAAAA CCCTTATTGT TATTCTCACT AACGAAGAAT ACTTGGAAGA TGCTAAGCTC
    TTAGAAAGAT ATATTGCAGA GGAGCTTAAC GTCCGTGAAG TAGTTTTCAC CTCCAATGAA
    GAGAAGTATG GAGTCGTATA CAGCGTTCAA GCAGATTGGC CTGTTTTGGG TAAGAAGCTT
    CGCAAAGATA TGGCTCGTGT TAAGAAGGCT CTACCAAATG TTACTTCTGA GGAAGTTAAA
    GAGTTTCAGA AGAACAAGAA AATGGTTTTG GATGGTATCG AACTTGTCGA GGGAGATTTA
    CAAATTATTC GTTCTGTGGA GGTTAAGAAC GAGTTTTTGA AATCGAACAC TGATGGCATC
    TGTATTGTTT TATTAGATAT AGAGATCGAT GCTCAGTTGC AAGCTGAAGG TTTGGCTCGC
    GAAGTTATCA ACCGTGTTCA ACGTTTGCGC AAGAAGAGCA ATTTACAAGT TACGGACGAT
    GTTCGTATGA CTTATAAAAT AAAAAACGAT ACTATTGGTC TCGAATCTGC TGTAGATTCC
    AATGAAGCCT TATTTTCGAA GGTATTGAGA AGACCCATCG AAAAGGAAAC TGGTGCTGAC
    GAGTCAAACA TAATTGCTTC CGAAGAACAA GACGTGCAAG GAGCTACCTT CCTTCTCAGT
    CTTCTTAGGC TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_595569.1
    CDS145..3339
    Translation

    Target ORF information:

    RefSeq Version NM_001021464.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative isoleucine--tRNA(Ile) ligase Irs1 (irs1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001021464.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCGTTTA ACGTTCCTAA AGAAGAGGAA AAAATCGTTG AGTTTTGGCG CGAAATCGAT 
    GCGTTCCATA CTCAGTTAAA ACTTTCTCAA GGTCGTCCTA CTTATACATT TTTCGATGGA
    CCTCCATTCG CTACCGGTCG TCCTCATCAT GGTCATCTTT TGGCTTCTAC CATTAAGGAT
    AGTGTCACCC GTTATGCTTG CTTGAAGGGT TATCACGTCG AGCGTAGATT CGGTTGGGAT
    ACACATGGAC TTCCTGTTGA GCATGAAATC GACAAAAAGT TGGGTATTAC TGGAAGTGAT
    GATGTTATGG CTATGGGTAT TGACAAATAC AATGCCGAAT GTCGTAAAAT TGTCATGACT
    TATGCATCCG AATGGCGTGC AACAGTTGAA AGACTGGGCC GTTGGATTGA TTTTGATAAC
    GATTATAAAA CGTTGTATCC TTCTTTCATG GAAAGTGTTT GGTGGGTTTT CAAAGAGTTG
    CATACAAAAG GAAAAGTTTA CCGCGGATAT CGTGTTATGC CTTACTCGAC TGCATGTACA
    ACACCTTTGA GTAACTTTGA AGCTCAACAA AACTATAAGG AAGTCCCAGA TCCGGCTATT
    GTTGTTGCCT TTCAATCCAT TAGTGATCCT GAAGTTTCTT TCTTAGCTTG GACTACTACT
    CCATGGACCT TGCCTTCTAA CCTTGCTTTG GCTGTTCACC CTGACTTACA GTACATAAAA
    ATTTTGGACA AGGATTCTAA TAAGAAATAT ATCCTTATGG AGAGCTGCCT TGGCATCCTT
    TACAAGAACC CAAAAAAGGC GAACTTTGAA ATTTTGGAAC GTTTCCAAGG AAAGGCCTTG
    GATGGTCAAA AGTATGAACC TCTCTTCCCT TATTTCAAGA GCACTTTTGG CGAAAGAGCA
    TTTAAATTAT ATTCTGCTGA CTACGTTGAA GAAGGCAGTG GTACTGGTAT CGTCCATCAA
    GCTCCTGCTT TTGGTGAAGC AGATTATGAC GCTGCTTGGG CTGCTGGCAT CATCGATGCT
    GATCACCAAC CTCCTTGCCC TGTTGATGAG CAAGGTTTGC TTACTTCGGA AATCACAGAC
    TTCGCTGGCC AATATGTTAA AGACGCAGAT AAGGAAATTA TCCGCTCCTT GAAAGCATCT
    GGCCATTTGG TTAAGCATAG TCAAATTTTC CATAGTTATC CATTTTGCTG GCGTTCCGAT
    ACACCTCTAA TTTATCGTGC CGTTCCCAGT TGGTTTGTTC GCGTGAAGGA GATCACTAAT
    GAGATGGTTG AAAATGTCAT GAGCACTCAC TGGGTCCCTC AAAACATTCG TGATAAACGT
    TTTGCGAATT GGTTGAAGAA TGCTCGTGAC TGGAACATTT CCCGTAACAG ATACTGGGGT
    ACCCCTATCC CACTTTGGGT TAGTGATGAC TACGAAGAAG TCGTTTGCAT TGGTTCCATT
    AAGGAGCTTG AAGAACTTTC CGGCGTTTCT AATATTACAG ACATTCATCG TGACAGTATT
    GATCATATCA CTATTCCTTC TAAAAAAGGT AAAGGAACAT TGCATCGTGT CAGTGAAGTA
    TTTGACTGTT GGTTTGAATC TGGCAGCATG CCTTATGCTT CTCGTCATTA CCCTTTCGAA
    AGAATTGAGG AATTCAAGCA TGGCTTCCCT GCTGACTTTA TTTCCGAGGG TGTTGATCAA
    ACCCGTGGAT GGTTTTATAC TTTAACTGTT TTGGGAACTT TGTTATTCGA CAAAGCACCT
    TATAAAAATG TAATTGTTAG TGGTCTGGTA ATGGCTGAGG ATGGTAAAAA AATGTCCAAA
    CGTCTTAAAA ATTACCCTGA ACCAAATCTC ATCATTGAAA AATATGGTTC TGATGCCCTT
    CGTTTGTATC TCATCAACTC TCCAGTTGTT CGTGCTGAGA TTCTTAAATT TAAGGAAGAT
    GGTGTAAGAG AAGTTGTTAC TAGAGTTTTG ATTCCTTGGT GGAACAGTTA TAAGTTTTTT
    GAGGCGCAAG CTGCTCTTTA CAAGAAGGTT ACTGGTAAAG ACTTTGTTTT CGATGATGCT
    GCCACTTTGA GTTCCAATGT AATGGATCGC TGGATTTTAG CTCGTTGCCA GAGCTTGATT
    GGATTTGTTG ATGAAGAAAT GAAACAATAC CGTTTGTACA CCGTGGTCCC TCAACTTCTC
    GGTTTAATTG AGGAGATGAC TAACTGGTAT ATCCGGTTTA ACAGACGTCG TTTGAAGGGT
    GAAGATGGTG AAATTGAAAC TATTAATGCT TTGAACGTTT TGTTTGAAGT CTTGTTCACG
    TTAGTTCGTA TTATGGGCCC ATTCACCCCT TTCATTACTG AAAACATTTA TCAACACCTT
    AGAAACTACA TGCCTATTGA CAAAAATGAA ATTTCACTTC GTTCAGTTCA TTTCCTTCCT
    TTCCCTACTT ACAAGTCTGA GCTTGATGAT GAAACTGTTT TACGCCGTGT TAAGCGTATG
    CAAACTATTA TCGAATTGGC GCGTTATGTT CGTGAGCAGA ATAATATTTC TTTGAAGACT
    CCCTTAAAAA CCCTTATTGT TATTCTCACT AACGAAGAAT ACTTGGAAGA TGCTAAGCTC
    TTAGAAAGAT ATATTGCAGA GGAGCTTAAC GTCCGTGAAG TAGTTTTCAC CTCCAATGAA
    GAGAAGTATG GAGTCGTATA CAGCGTTCAA GCAGATTGGC CTGTTTTGGG TAAGAAGCTT
    CGCAAAGATA TGGCTCGTGT TAAGAAGGCT CTACCAAATG TTACTTCTGA GGAAGTTAAA
    GAGTTTCAGA AGAACAAGAA AATGGTTTTG GATGGTATCG AACTTGTCGA GGGAGATTTA
    CAAATTATTC GTTCTGTGGA GGTTAAGAAC GAGTTTTTGA AATCGAACAC TGATGGCATC
    TGTATTGTTT TATTAGATAT AGAGATCGAT GCTCAGTTGC AAGCTGAAGG TTTGGCTCGC
    GAAGTTATCA ACCGTGTTCA ACGTTTGCGC AAGAAGAGCA ATTTACAAGT TACGGACGAT
    GTTCGTATGA CTTATAAAAT AAAAAACGAT ACTATTGGTC TCGAATCTGC TGTAGATTCC
    AATGAAGCCT TATTTTCGAA GGTATTGAGA AGACCCATCG AAAAGGAAAC TGGTGCTGAC
    GAGTCAAACA TAATTGCTTC CGAAGAACAA GACGTGCAAG GAGCTACCTT CCTTCTCAGT
    CTTCTTAGGC TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L