Gene Symbol | pfh1 |
Entrez Gene ID | 2541213 |
Full Name | DNA repair and recombination protein |
Gene Type | protein-coding |
Organism | Schizosaccharomyces pombe(fission yeast) |
ORF » Species Summary » Schizosaccharomyces pombe » pfh1 cDNA ORF clone
Gene Symbol | pfh1 |
Entrez Gene ID | 2541213 |
Full Name | DNA repair and recombination protein |
Gene Type | protein-coding |
Organism | Schizosaccharomyces pombe(fission yeast) |
mRNA | Protein | Name |
---|---|---|
NM_001022408.2 | NP_596488.1 | DNA repair and recombination protein |
The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
Pfh1 is an accessory DNA helicase that interacts with the replisome and promotes replication and suppresses DNA damage at hard-to-replicate sites.
Title: Pfh1 Is an Accessory Replicative Helicase that Interacts with the Replisome to Facilitate Fork Progression and Preserve Genome Integrity.
In budding and fission yeasts, Pif1 family helicases impact both telomerase-mediated and semi-conservative replication of telomeric DNA as well as recombination-mediated telomere lengthening. (Review)
Title: Getting it done at the ends: Pif1 family DNA helicases and telomeres.
The nuclear isoform of Pfh1 (nPfh1) unwound both types of structure, and although the G4-stabilizing compound Phen-DC3 significantly enhanced their stability, nPfh1 still resolved them efficiently
Title: G-rich telomeric and ribosomal DNA sequences from the fission yeast genome form stable G-quadruplex DNA structures in vitro and are unwound by the Pfh1 DNA helicase.
Pfh1 binds telomeric DNA.Pfh1 facilitates telomere replication.Pfh1 is a positive regulator of telomere length.
Title: The Pif1 family helicase Pfh1 facilitates telomere replication and has an RPA-dependent role during telomere lengthening.
The proteome of cells devoid of Pfh1 were analyzed and determined that gene products up- and down-regulated upon iron depletion in wild-type cells are constitutively misregulated in this mutant.
Title: Cells lacking pfh1, a fission yeast homolog of mammalian frataxin protein, display constitutive activation of the iron starvation response.
The following pfh1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pfh1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OSc100936 | |
Clone ID Related Accession (Same CDS sequence) | NM_001022408.2 | |
Accession Version | NM_001022408.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2018-04-02 | |
Organism | Schizosaccharomyces pombe(fission yeast) | |
Product | DNA repair and recombination protein | |
Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022408.1. |
1 | ATGTTTTCGT GCCAATCTCT TTATAAATTT AGTCACTCTT TTAGGAAACG TATCCCCGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_596488.1 |
CDS | 368..2785 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001022408.2 |
1 | ATGTTTTCGT GCCAATCTCT TTATAAATTT AGTCACTCTT TTAGGAAACG TATCCCCGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The genome sequence of Schizosaccharomyces pombe. |