mcs4 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	mcs4
Entrez Gene ID	2541209
Full Name	response regulator Mcs4
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022404.2	NP_596484.1	response regulator Mcs4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that Mcs4 is required for stable heteromer formation between the Wis4 and Win1 MAPKKKs.
Title: Response regulator-mediated MAPKKK heteromer promotes stress signaling to the Spc1 MAPK in fission yeast.

results suggest that S. pombe has an alternative module(s) that directs the methylglyoxal signal to the SAPK pathway via Mcs4
Title: Methylglyoxal as a signal initiator for activation of the stress-activated protein kinase cascade in the fission yeast Schizosaccharomyces pombe.

The following mcs4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mcs4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100932	NM_001022404.2 Latest version!	Schizosaccharomyces pombe response regulator Mcs4 (mcs4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mcs4 ( NM_001022404.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596484.1 Schizosaccharomyces pombe response regulator Mcs4 (mcs4), mRNA.

Price & Availability↑

CloneID	OSc100932
Clone ID Related Accession (Same CDS sequence)	NM_001022404.2
Accession Version	NM_001022404.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	response regulator Mcs4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022404.1.

ATGCGCATTT GGTTTAAAAA AGTTCCAGAT GGGATTACTT CCTCCGTTAT ATTGTCTGAA 
GATCATCTGG TTGACGATTT AAAAGATGCC ATCGCTCGGA AATTCCCTAT TCGTATCAGT 
CAGTATTATG ATGCACCTGA GCTTTCGATT CGTGTAGTAG CTCCACCAAA TGCATCATCG 
GAGTTGCAAT CTAGAGAACT CAGTCCCAAC GAAAGCATAT TATTTGTCAT GGAAACTTAT 
TATCCTCATG GTCAGGATTT TAACGACGCG CTTCTAGTAG CGTCGCCGGA TACCTCAGTT 
GCCTTAAGAT ATCGCTCTTC TCAACTCTCA TCCTCTACAT TTGAATCAAC ACCTCCCGTT 
TTTTCTGAAT ACCCACCTAA CATAATCCCT ACCCCAGCGA ACGAAACAGT CCCGCGTATC 
AAACAGCCAT CCATTGCTCT TGATTCACTT GAGAGCCCGG TTTCTGCCCC TTCACGACAT 
CAAAGTACTT ATTCTTATAA AGGAGGTCCT TTAAATTATA ATTTACGAAA TGCATCCCGA 
ACTAGGTCCC ATCAAACTCT TCCTTCCTCT AATGTAAATA AAACTGGCGT ACTACTTTTG 
CCTCGTTCTT CTAGACAGCA AACATTGGCT TCAAGACCCT CTTTACCAGA TCTAACTTCA 
GCTGACAAGT CGCAACCATC AGACGAAGCC GAATCCATTA CTAGAAAAAA TTCTATTGGA 
ATGTCGACTC GGTCTGATGA ATCAACAGCT GAAAAATTGG CGAAAGCCGA AGTCGCGACA 
CCCACTAATA GTAGAAGTAT TAGTCATTCA TCGCTTTATA CGAAACAATC TGGTACCGCA 
GGAGTCCTTC CCGCGGTTAA TGCTGATATT GACGCAGCAA ATAGGATGAA CCCTGATATC 
AGTTCTCAAT TTCCTATAGC AGACAACAAA GATCCCTTAA ATGCTGATAC ACAAGCCCAT 
TTAGGATTTC CTTCTAATCA AATAGATGGA ATTGTTGGTA CTTCACCAGT CAATGTTCTA 
ACAAGTCCCG GCATAGGTGC GAAAGCACCT TTTGCTAGTC TACTTGAAGG AGTGATTCCT 
CCAATTAACG TTTTGATTGT TGAAGATAAT ATTATTAATC AAAAAATCCT AGAGACTTTT 
ATGAAGAAGC GCAATATTTC CTCGGAGGTT GCTAAAGATG GGCTTGAAGC ACTCGAGAAG 
TGGAAAAAGA AATCTTTTCA CTTGATTCTA ATGGACATCC AACTTCCCAC GATGTCCGGT 
ATTGAAGTCA CCCAAGAAAT TAGGAGACTT GAGCGGTTAA ACGCAATTGG AGTTGGTGCT 
CCAAAATTGA CACAACCGAT ACCTGAAAAG GATCAGCTAA ATGAAAACAA ATTTCAATCT 
CCTGTGATTA TAGTGGCACT TACTGCTTCT AGCCTAATGG CTGATCGAAA CGAGGCTTTA 
GCTGCTGGTT GCAATGATTT TCTTACAAAG CCTGTCTCTT TAGTTTGGCT AGAGAAAAAA 
ATTACGGAGT GGGGCTGTAT GCAAGCGCTT ATTGATTGGA ATGGTTGGTG CCGTTTTCGC 
GGTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596484.1
CDS	185..1753
Translation	MRIWFKKVPDGITSSVILSEDHLVDDLKDAIARKFPIRISQYYDAPELSIRVVAPPNASSELQSRELSPNESILFVMETYYPHGQDFNDALLVASPDTSVALRYRSSQLSSSTFESTPPVFSEYPPNIIPTPANETVPRIKQPSIALDSLESPVSAPSRHQSTYSYKGGPLNYNLRNASRTRSHQTLPSSNVNKTGVLLLPRSSRQQTLASRPSLPDLTSADKSQPSDEAESITRKNSIGMSTRSDESTAEKLAKAEVATPTNSRSISHSSLYTKQSGTAGVLPAVNADIDAANRMNPDISSQFPIADNKDPLNADTQAHLGFPSNQIDGIVGTSPVNVLTSPGIGAKAPFASLLEGVIPPINVLIVEDNIINQKILETFMKKRNISSEVAKDGLEALEKWKKKSFHLILMDIQLPTMSGIEVTQEIRRLERLNAIGVGAPKLTQPIPEKDQLNENKFQSPVIIVALTASSLMADRNEALAAGCNDFLTKPVSLVWLEKKITEWGCMQALIDWNGWCRFRGR

Target ORF information:

RefSeq Version	NM_001022404.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe response regulator Mcs4 (mcs4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022404.2

ORF Insert Sequence:

ATGCGCATTT GGTTTAAAAA AGTTCCAGAT GGGATTACTT CCTCCGTTAT ATTGTCTGAA 
GATCATCTGG TTGACGATTT AAAAGATGCC ATCGCTCGGA AATTCCCTAT TCGTATCAGT 
CAGTATTATG ATGCACCTGA GCTTTCGATT CGTGTAGTAG CTCCACCAAA TGCATCATCG 
GAGTTGCAAT CTAGAGAACT CAGTCCCAAC GAAAGCATAT TATTTGTCAT GGAAACTTAT 
TATCCTCATG GTCAGGATTT TAACGACGCG CTTCTAGTAG CGTCGCCGGA TACCTCAGTT 
GCCTTAAGAT ATCGCTCTTC TCAACTCTCA TCCTCTACAT TTGAATCAAC ACCTCCCGTT 
TTTTCTGAAT ACCCACCTAA CATAATCCCT ACCCCAGCGA ACGAAACAGT CCCGCGTATC 
AAACAGCCAT CCATTGCTCT TGATTCACTT GAGAGCCCGG TTTCTGCCCC TTCACGACAT 
CAAAGTACTT ATTCTTATAA AGGAGGTCCT TTAAATTATA ATTTACGAAA TGCATCCCGA 
ACTAGGTCCC ATCAAACTCT TCCTTCCTCT AATGTAAATA AAACTGGCGT ACTACTTTTG 
CCTCGTTCTT CTAGACAGCA AACATTGGCT TCAAGACCCT CTTTACCAGA TCTAACTTCA 
GCTGACAAGT CGCAACCATC AGACGAAGCC GAATCCATTA CTAGAAAAAA TTCTATTGGA 
ATGTCGACTC GGTCTGATGA ATCAACAGCT GAAAAATTGG CGAAAGCCGA AGTCGCGACA 
CCCACTAATA GTAGAAGTAT TAGTCATTCA TCGCTTTATA CGAAACAATC TGGTACCGCA 
GGAGTCCTTC CCGCGGTTAA TGCTGATATT GACGCAGCAA ATAGGATGAA CCCTGATATC 
AGTTCTCAAT TTCCTATAGC AGACAACAAA GATCCCTTAA ATGCTGATAC ACAAGCCCAT 
TTAGGATTTC CTTCTAATCA AATAGATGGA ATTGTTGGTA CTTCACCAGT CAATGTTCTA 
ACAAGTCCCG GCATAGGTGC GAAAGCACCT TTTGCTAGTC TACTTGAAGG AGTGATTCCT 
CCAATTAACG TTTTGATTGT TGAAGATAAT ATTATTAATC AAAAAATCCT AGAGACTTTT 
ATGAAGAAGC GCAATATTTC CTCGGAGGTT GCTAAAGATG GGCTTGAAGC ACTCGAGAAG 
TGGAAAAAGA AATCTTTTCA CTTGATTCTA ATGGACATCC AACTTCCCAC GATGTCCGGT 
ATTGAAGTCA CCCAAGAAAT TAGGAGACTT GAGCGGTTAA ACGCAATTGG AGTTGGTGCT 
CCAAAATTGA CACAACCGAT ACCTGAAAAG GATCAGCTAA ATGAAAACAA ATTTCAATCT 
CCTGTGATTA TAGTGGCACT TACTGCTTCT AGCCTAATGG CTGATCGAAA CGAGGCTTTA 
GCTGCTGGTT GCAATGATTT TCTTACAAAG CCTGTCTCTT TAGTTTGGCT AGAGAAAAAA 
ATTACGGAGT GGGGCTGTAT GCAAGCGCTT ATTGATTGGA ATGGTTGGTG CCGTTTTCGC 
GGTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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mcs4 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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