fab1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	fab1
Entrez Gene ID	2541154
Full Name	1-phosphatidylinositol-3-phosphate 5-kinase Fab1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022005.3	NP_596090.2	1-phosphatidylinositol-3-phosphate 5-kinase Fab1

Pathways from BioSystems

Homologies

Magnaporthe oryzae (rice blast fungus)	MGG_14757	XP_003713896.1
Neurospora crassa	NCU02083	XP_964610.1
Schizosaccharomyces pombe (fission yeast)	fab1	NP_596090.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following fab1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fab1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100068	NM_001022005.3 Latest version!	Schizosaccharomyces pombe 1-phosphatidylinositol-3-phosphate 5-kinase Fab1 (fab1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

fab1 ( NM_001022005.3 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596090.2 Schizosaccharomyces pombe 1-phosphatidylinositol-3-phosphate 5-kinase Fab1 (fab1), mRNA.

Price & Availability↑

CloneID	OSc100068
Clone ID Related Accession (Same CDS sequence)	NM_001022005.3
Accession Version	NM_001022005.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	1-phosphatidylinositol-3-phosphate 5-kinase Fab1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022005.2.

ATGTCAGAAG TCGAAACGCC TACCGCAGCC TCGCCCACTT TTCCTGTTGA AACCTCTCAC 
CGTTTAGATA CTTTGCATAC ATCAAGCACA GAGCAAATCA TTAAAGATTC CGAGAATGTT 
GTCCACACTA CGTTGAAATT GCCGACTAGT ACGTTGTCGC GCGAATTTTG GATGAAAGAT 
GAAAGAACAA ACAACTGTTC ATTATGTGAG ACGGAATTTA CTTTATTTCG AAGGAAACAT 
CATTGTAGAA TATGTGGGAA AATTATCTGC AAATATTGCT TAAAAGAAGC TCCTGGATTT 
ATCTTTCGTC TCCAAGGATC GATCAAAGTG TGTCGTCCAT GTGCTTCAAT TTTAGTTAAT 
AATTATTCTC GAAGCCAGCT ATTCAATCAT TCATTAAACG AATCCAAAAA TAGAGATCTT 
ACGGAGCAAC ATCCGTTTGT TACACTGGAC GAACTTAATT CGAATGATCA AGTACTATCA 
TCATTTGGAG ATTTAAGTAG CACATTTGAG ATGCCTAATA ATATTCATCC TCCTGAAGTT 
GCTCCAATGA TAGCTATTCC CTCTAGTCGC TCGAATTATG ATTCTCCTGG TTGGGCTCAC 
CATTCCATCT TTCTTGACTG GAGCAAAAGG AATTTAGATT CGAATGTTAT AAATGTTGAA 
GATTCCGAAA GTGGTAAATA TAATGCTTTA ACCATTACAA ATAGTTACGA TGCTGGTCCT 
AGCTCTGTTT CCACAGATTA TCGTCCCGTC AATTTTGGAA AAGTACCTTC ATACTCAAAG 
TTAAGAAAGA ATAAAGCGTT TTCCTCAGCG AAAGTCTCTG ATATGTACTT GTCGGCTGAT 
GAAAGAAACC GGCTCGAGGA TTTTAGCAAA GGCGACAGAG GCCTTTCATT TGTTAACCTT 
TCTCCAAATA TAAAAGCAAC GTCTTACGAT CGATTGAGCA CAGTAATAAA TGAACCCTTT 
ATTTCAAGAA GTTCTTCCTT GACTGATGAA CGGGGTTTGG CCGATTCGGG CAATTCCTAT 
CATCATTTCT CCGACTCCGA CGATGAATCA CTATTCAATG ATGGATTGGG CCTTTCTTTT 
CATGCCAATA GTGCAATTAT TAAGCAACGC CAGCAAAATG TTGCATCAAT TCAGCGTTAC 
GGTAATGAAA GTTACTTATC AAATTTTTTA AAAGCTTTTC TTCCGAAAAC GGTTTGCGAC 
TATCTTTTTC CTTCTTCTAC TATTCCCGAT GGACTTCCCG CTTTAATTGA AAACTTCAAT 
GCGCGAGTTG ATAAGGTGAA TCATCCCGGT GGAACAGAAG AGCCTCTTCC TTACCAGGGA 
AAGTCGCGTG CTTCTTCTGT AGTTACCTCT TCCAAAAGCA CATGTATTTT ACCACCTTGG 
ATTCTCTTCT CTGATTCATT TAATCAGCTT GTATGTACTT TTCTGGGAAA ATTACTCTTT 
CAAATGCTAA ATGATGAGGG TGTGGATTCG CCTATGCAAT GGGTACTTTG TCTCCCAAAA 
ATTCTTTTAA AAATGGCTTT GGATCTAGGT CCTGATATTC GCTCTGGTGA TGATATTGAT 
GTCCGCAGCT ATGTAAAAAT AAAAAAGATT CCCGGGGGAA GCATTCAAGA TTGTTTTCTT 
GTTAATGGTG TTTTGTTCTC AAAAAAAGCT TCGTCCAAAT CTATGGACCG CTCGCTTCGT 
CGGCCTCGTA TAGCTCTTTT AACTTTCAGT TTAGACTACG CTTGTGACGA ACAGAGAATT 
TTATCCTTGG ATTTAATCAT CTCTCAGCAA GAAGAGTATA TTATAAATCT TGTCAATAGA 
ATCTGCATGC TTAAACCGAA TCTAGTTTTT GCTCAGGGTC AAATCCCCAG TATTGCCTTG 
AAGTACTTTG AAGAACATGG CGTTATAGCC TTTCATGGAC TTAAAGAATC AGTTTTATAT 
GACATAGCTA GATGTTGTCG TGCAGACATA ATATCGTCAA TAGATAAATT GTCGCTTTGT 
CCTCGTCTTG GTACTTGCGG CAGGTTTCAA CTCAGAACGT ATGTTGTCGA TGAAAACAAA 
GGGTTGAGGA AAACGTTTGC TATACTTGAT CGCTGCTCGG AACGTTTAGG CTGCACCATA 
GTTCTAAGAG GAGCTGATTA TAATCAGTTG AGCAAGGTCA AGAAGATTGT CGAACTTGTT 
GTTTTGATTG CATATCATAT CAAACTTGAA TGTGCCTTGT TAAGAGACAA ATTTGTGAAT 
ATGCCGGAGC TTTTTGAAAC AACCTATCAA AGTTTGTCTA GGAAATCGTT GCCTTCGTTT 
GCCTCTACTG CAGCTGATAA AGAGAAAAGC CAAAATCATG AAAAAAAATC TCTGAACAGT 
GATAATCAAA GTTTGCGACC TTTGGAAAAT GAAAATCAAA GTGTTTCTAG CACCCAAGGT 
AGCAACAGCC CGCTTGAGTT GATCAATAAC TTACCAGCCT CAGATGACTA TTCTTCTATT 
ACCAAGGCTT TGAAAACCAG ATTTTTAACG TTTTCACCCT TTTTATCAAA GCCTCTTCCA 
CGTTTATTAA ACCAAGTTAA TTATTACCAG TTTATTCGTA ACAAATTACT GAAAGATGTG 
AAGTTACATC CATACTCACC AACTGGCTCA TTCGTAATGA AGCAGTCCGA AAATGACAAT 
GTAGAAGAAA GTTACGAAGA GTCCTATAAA TTCTTTTGTA TTGATGAACG TTATCATTTT 
TTAGAAAAGC AGTGGACACT TTATTACTCT CATTCAAAAC TCATGTTTTC TCCATTTTCT 
TCTCAAAGGA TTATTTTGTT ATATTCCATT ATTAACAAGG AAACTTCTGT TCCTTGTATT 
GGCCCTGAAC GTTGTCTATT AGAATTTTAT CGAGAAACTG ATTGCACTCT TGGTCAGTAC 
ATAGAGGACT CATGCTTGAA TACCAATGTA AGTTGTGGAG GTGAGTATTG TAAAACGAAT 
GATATGCTGT GGCATTACAG GAGTTATGTT CATGGAAATA GTCGGATCAG CGTTTTTTTG 
GAATCTTTTT CGTGCCCTGT CCCTGGTTTG GAAGAAAAGA TAATTATGTG GAGTTACTGC 
AAATTCTGTA AAAAGAATAC ACACATTACT GTTATGTCTG AAGAAACATG GAAATATTCG 
TTTGGAAAAT ATCTTGAATT CATGTTCTAT AATTCTCAAA TAAGGGATCG CTTCGAATTC 
TGTGACCACA GTGTTATGGC ACAACATGTT CATTATTTCG GTTATTGCAA CATGGCTTTA 
CGATTTCAAA GAGACCTAAT AGAGATTTTT GAACTCTTCG TTCCATCGGT CACTTTACGA 
AATAATCCAT CATATATTAA AGAGCTGAAA GAAAAGGAGT ATAAACGTTT AAAAGGTGTC 
ATTGAAAAAT GCTTGTCTTC TGTTGCTTCA AGGATAAACC AAATAAAGTG CGATTGGGTT 
ACTGATCCTG AGAAATTTGA GTCTTGTACA TCTGAAATTT CAAAATTTCG CACTTTACTT 
TCAAGTGACT ATACTGAATT GTATTCTGAA TTTGATTCAA TTTATCTTAA TTCCTCAACT 
TCTGATTATT TATCACTAAA CTCAATCCTG CGCGTCCTCC AGGGTAAAAT GGTAAAATGG 
GAACAGCGCT TTCTTGACTA TCAACGGCTG TATCTTCCAT CTTACAAAGA GTTAAGTAAA 
ATAGCAGCCG CTCAAATTAA GAAAGTGTTT CTGGAACGTC CATTAAGCCA AACCCCTTTA 
GATTTGCCAG AGACACTTGA GAATACTCAA ATTGATATAT ATCCCTCCTT TAAAACTGAA 
TCCACTGATG ATCAACTCGA GAAAGTTACC CAAACTAATG TTGCTTCAAA CAAGCGTGTT 
GCTCCGTATG CAGATAGTAT GGCAAATGTT GGTTCTCCAG AATCTGATTG CTTTTCAGTA 
GCAACATCTT CCGATATTCC TAAAGCCAAC ATCGATTTTA CCAATGATAT TTCTACTCAG 
AACACTTTTC CAGCTTCTCC GGTCTCAAAT TCAGGTTTCA GTAGACAAAC GTATCCAAAT 
ATAAGCCAAA GACAAGGCGT TAATATGCTT TCTCATAAGC GGAAAAGTGC TAGTACCAGT 
GACAGGCGAT TTGTCAATGC ATCTTCAACA AGTGGGATGA ACATGCCTAT TTCTTCTTCA 
ATATCTGCAA AAATTTCCAG TATACAGAAT TCTACAAAAT ACTCACCCAG GAAGCCCATT 
CCAGCAAAAG ATGTGCGGGT TAGCTCTTTA GTGCGTAGAT TTGAAGAGCT AAGTTTACAA 
TTACAAGAAA AGCAAAAACG AGATGAAGAA TTAATAAAGG CTCGACGCAA GCGGGCTCTT 
CCTGTAGTCC CATCTAAGCC GGTAGTAGAA GTATTTAATG ACCTTAATGA AGCATTTGAT 
GATGAAAATT CCGAAGATGA GAATGGCATT AATGACACAA AAGAAAATCG CGCTACCGAA 
AGCAATTTTT CTGGAGTTGA TTCTATGAGC AAGGAGCGAG AAAATGTTTC TTCGAATGAA 
GACAATTCAC CTGAAGCGTT TGAAGATATT TTTGGAATTC TTTTCAAAAA TGAATCTGGC 
CTCGAAGAAC AGCAAAACCT TGAACCTTCT AGTCAAATGG ATAAAGAGGG ATCAAAGTTG 
CCAACTTCTG GTCCTTTGGC TGACAAAACT TCAGTTTACA GGATTTTATC TGCATTTTGG 
AATGAATGGA ACTCCCTCAA TCCTCCACCT TTTGAATTTC CTCTACAACC TACAGAGCAT 
ATGTTTTCCG ACTCAAATGT GATCATAAGG GAAGACGAAC CTAGCTCCTT GATAAGTTTT 
ACTCTTAGCT CTCCTGACTA TTTATCAAAA ATGGTAGAAA TTGAAGATAG CATGGACGAA 
GCTTTAACCA ATCAAGGCTT ACAAGGCTCG ACGCAATTCA AAATTGAAAA CTTAATGCTC 
AAACCAACGG GGACGCATTT GAAATACCAG TTTGAAGAAG GATCAGCAAG ACTATCCTGT 
AAGGTCTTTT TCGCGGAACA ATTTAGTGCT TTGCGAAGAG CTTGTGGCTG TGAGGAGACG 
TTTGTGACAT CTCTAGCGAG ATGTTCTCTT TGGGAATCTA GCGGTGGAAA ATCTGGATCA 
GCATTTTTAA AAACTTTCGA TAAGAAATAC ATTCTCAAAG TTCTGTCAAG GTTGGAAAGT 
GACTCCTTGC TTAATTTTGC TCCAGCTTAT TTTGATTACA TATCTAAAGT TTTCTTTCAC 
GAGTTGCCAA CTGCATTGAC TAAAATCTTT GGATTTTACC GCGTAGACAT TCGAAATCCT 
ACTACTGGTA CAATCTGTAA GACTGATATA ATGATTATGG AAAATGTATT TTATGATGAA 
TGTCCGTCTA GAATATTTGA TCTTAAAGGT TCCATGAGGA ATCGACATGT AGAATCTACA 
GGGAAGGTAG ATGAAGTATT GCTTGATGAA AATTTGGTTG AACTTATTTA CGAATCTCCA 
ATATTTGTTA GTGAACAACT AAAAAGTCTT TTGCATTCTT GCCTTTGGAA TGATACATTG 
TTCCTCTCAA AACTCAATAT CATGGATTAC TCTTTAATTG TTGGTATTGA TTATACAAAA 
AAAGAACTTT ATGTTGGAAT AATTGATTTC ATACGAACAT ACACTTGGGA CAAAAAACTT 
GAAAGTTGGG TAAAGGAGAA AGGCTTAGTT GGTAGAGGTC CTGAACCAAC AATTGTTACT 
CCAAAGCAAT ACAAAAATAG ATTCCGAAAG GCTATGGATT GCTATATTTT AGCATCTCAG 
GATTTTGAGA CTGGGGAAGG TTTTAAGTTT TGCGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596090.2
CDS	117..5915
Translation	MSEVETPTAASPTFPVETSHRLDTLHTSSTEQIIKDSENVVHTTLKLPTSTLSREFWMKDERTNNCSLCETEFTLFRRKHHCRICGKIICKYCLKEAPGFIFRLQGSIKVCRPCASILVNNYSRSQLFNHSLNESKNRDLTEQHPFVTLDELNSNDQVLSSFGDLSSTFEMPNNIHPPEVAPMIAIPSSRSNYDSPGWAHHSIFLDWSKRNLDSNVINVEDSESGKYNALTITNSYDAGPSSVSTDYRPVNFGKVPSYSKLRKNKAFSSAKVSDMYLSADERNRLEDFSKGDRGLSFVNLSPNIKATSYDRLSTVINEPFISRSSSLTDERGLADSGNSYHHFSDSDDESLFNDGLGLSFHANSAIIKQRQQNVASIQRYGNESYLSNFLKAFLPKTVCDYLFPSSTIPDGLPALIENFNARVDKVNHPGGTEEPLPYQGKSRASSVVTSSKSTCILPPWILFSDSFNQLVCTFLGKLLFQMLNDEGVDSPMQWVLCLPKILLKMALDLGPDIRSGDDIDVRSYVKIKKIPGGSIQDCFLVNGVLFSKKASSKSMDRSLRRPRIALLTFSLDYACDEQRILSLDLIISQQEEYIINLVNRICMLKPNLVFAQGQIPSIALKYFEEHGVIAFHGLKESVLYDIARCCRADIISSIDKLSLCPRLGTCGRFQLRTYVVDENKGLRKTFAILDRCSERLGCTIVLRGADYNQLSKVKKIVELVVLIAYHIKLECALLRDKFVNMPELFETTYQSLSRKSLPSFASTAADKEKSQNHEKKSLNSDNQSLRPLENENQSVSSTQGSNSPLELINNLPASDDYSSITKALKTRFLTFSPFLSKPLPRLLNQVNYYQFIRNKLLKDVKLHPYSPTGSFVMKQSENDNVEESYEESYKFFCIDERYHFLEKQWTLYYSHSKLMFSPFSSQRIILLYSIINKETSVPCIGPERCLLEFYRETDCTLGQYIEDSCLNTNVSCGGEYCKTNDMLWHYRSYVHGNSRISVFLESFSCPVPGLEEKIIMWSYCKFCKKNTHITVMSEETWKYSFGKYLEFMFYNSQIRDRFEFCDHSVMAQHVHYFGYCNMALRFQRDLIEIFELFVPSVTLRNNPSYIKELKEKEYKRLKGVIEKCLSSVASRINQIKCDWVTDPEKFESCTSEISKFRTLLSSDYTELYSEFDSIYLNSSTSDYLSLNSILRVLQGKMVKWEQRFLDYQRLYLPSYKELSKIAAAQIKKVFLERPLSQTPLDLPETLENTQIDIYPSFKTESTDDQLEKVTQTNVASNKRVAPYADSMANVGSPESDCFSVATSSDIPKANIDFTNDISTQNTFPASPVSNSGFSRQTYPNISQRQGVNMLSHKRKSASTSDRRFVNASSTSGMNMPISSSISAKISSIQNSTKYSPRKPIPAKDVRVSSLVRRFEELSLQLQEKQKRDEELIKARRKRALPVVPSKPVVEVFNDLNEAFDDENSEDENGINDTKENRATESNFSGVDSMSKERENVSSNEDNSPEAFEDIFGILFKNESGLEEQQNLEPSSQMDKEGSKLPTSGPLADKTSVYRILSAFWNEWNSLNPPPFEFPLQPTEHMFSDSNVIIREDEPSSLISFTLSSPDYLSKMVEIEDSMDEALTNQGLQGSTQFKIENLMLKPTGTHLKYQFEEGSARLSCKVFFAEQFSALRRACGCEETFVTSLARCSLWESSGGKSGSAFLKTFDKKYILKVLSRLESDSLLNFAPAYFDYISKVFFHELPTALTKIFGFYRVDIRNPTTGTICKTDIMIMENVFYDECPSRIFDLKGSMRNRHVESTGKVDEVLLDENLVELIYESPIFVSEQLKSLLHSCLWNDTLFLSKLNIMDYSLIVGIDYTKKELYVGIIDFIRTYTWDKKLESWVKEKGLVGRGPEPTIVTPKQYKNRFRKAMDCYILASQDFETGEGFKFCE

Target ORF information:

RefSeq Version	NM_001022005.3
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe 1-phosphatidylinositol-3-phosphate 5-kinase Fab1 (fab1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022005.3

ORF Insert Sequence:

ATGTCAGAAG TCGAAACGCC TACCGCAGCC TCGCCCACTT TTCCTGTTGA AACCTCTCAC 
CGTTTAGATA CTTTGCATAC ATCAAGCACA GAGCAAATCA TTAAAGATTC CGAGAATGTT 
GTCCACACTA CGTTGAAATT GCCGACTAGT ACGTTGTCGC GCGAATTTTG GATGAAAGAT 
GAAAGAACAA ACAACTGTTC ATTATGTGAG ACGGAATTTA CTTTATTTCG AAGGAAACAT 
CATTGTAGAA TATGTGGGAA AATTATCTGC AAATATTGCT TAAAAGAAGC TCCTGGATTT 
ATCTTTCGTC TCCAAGGATC GATCAAAGTG TGTCGTCCAT GTGCTTCAAT TTTAGTTAAT 
AATTATTCTC GAAGCCAGCT ATTCAATCAT TCATTAAACG AATCCAAAAA TAGAGATCTT 
ACGGAGCAAC ATCCGTTTGT TACACTGGAC GAACTTAATT CGAATGATCA AGTACTATCA 
TCATTTGGAG ATTTAAGTAG CACATTTGAG ATGCCTAATA ATATTCATCC TCCTGAAGTT 
GCTCCAATGA TAGCTATTCC CTCTAGTCGC TCGAATTATG ATTCTCCTGG TTGGGCTCAC 
CATTCCATCT TTCTTGACTG GAGCAAAAGG AATTTAGATT CGAATGTTAT AAATGTTGAA 
GATTCCGAAA GTGGTAAATA TAATGCTTTA ACCATTACAA ATAGTTACGA TGCTGGTCCT 
AGCTCTGTTT CCACAGATTA TCGTCCCGTC AATTTTGGAA AAGTACCTTC ATACTCAAAG 
TTAAGAAAGA ATAAAGCGTT TTCCTCAGCG AAAGTCTCTG ATATGTACTT GTCGGCTGAT 
GAAAGAAACC GGCTCGAGGA TTTTAGCAAA GGCGACAGAG GCCTTTCATT TGTTAACCTT 
TCTCCAAATA TAAAAGCAAC GTCTTACGAT CGATTGAGCA CAGTAATAAA TGAACCCTTT 
ATTTCAAGAA GTTCTTCCTT GACTGATGAA CGGGGTTTGG CCGATTCGGG CAATTCCTAT 
CATCATTTCT CCGACTCCGA CGATGAATCA CTATTCAATG ATGGATTGGG CCTTTCTTTT 
CATGCCAATA GTGCAATTAT TAAGCAACGC CAGCAAAATG TTGCATCAAT TCAGCGTTAC 
GGTAATGAAA GTTACTTATC AAATTTTTTA AAAGCTTTTC TTCCGAAAAC GGTTTGCGAC 
TATCTTTTTC CTTCTTCTAC TATTCCCGAT GGACTTCCCG CTTTAATTGA AAACTTCAAT 
GCGCGAGTTG ATAAGGTGAA TCATCCCGGT GGAACAGAAG AGCCTCTTCC TTACCAGGGA 
AAGTCGCGTG CTTCTTCTGT AGTTACCTCT TCCAAAAGCA CATGTATTTT ACCACCTTGG 
ATTCTCTTCT CTGATTCATT TAATCAGCTT GTATGTACTT TTCTGGGAAA ATTACTCTTT 
CAAATGCTAA ATGATGAGGG TGTGGATTCG CCTATGCAAT GGGTACTTTG TCTCCCAAAA 
ATTCTTTTAA AAATGGCTTT GGATCTAGGT CCTGATATTC GCTCTGGTGA TGATATTGAT 
GTCCGCAGCT ATGTAAAAAT AAAAAAGATT CCCGGGGGAA GCATTCAAGA TTGTTTTCTT 
GTTAATGGTG TTTTGTTCTC AAAAAAAGCT TCGTCCAAAT CTATGGACCG CTCGCTTCGT 
CGGCCTCGTA TAGCTCTTTT AACTTTCAGT TTAGACTACG CTTGTGACGA ACAGAGAATT 
TTATCCTTGG ATTTAATCAT CTCTCAGCAA GAAGAGTATA TTATAAATCT TGTCAATAGA 
ATCTGCATGC TTAAACCGAA TCTAGTTTTT GCTCAGGGTC AAATCCCCAG TATTGCCTTG 
AAGTACTTTG AAGAACATGG CGTTATAGCC TTTCATGGAC TTAAAGAATC AGTTTTATAT 
GACATAGCTA GATGTTGTCG TGCAGACATA ATATCGTCAA TAGATAAATT GTCGCTTTGT 
CCTCGTCTTG GTACTTGCGG CAGGTTTCAA CTCAGAACGT ATGTTGTCGA TGAAAACAAA 
GGGTTGAGGA AAACGTTTGC TATACTTGAT CGCTGCTCGG AACGTTTAGG CTGCACCATA 
GTTCTAAGAG GAGCTGATTA TAATCAGTTG AGCAAGGTCA AGAAGATTGT CGAACTTGTT 
GTTTTGATTG CATATCATAT CAAACTTGAA TGTGCCTTGT TAAGAGACAA ATTTGTGAAT 
ATGCCGGAGC TTTTTGAAAC AACCTATCAA AGTTTGTCTA GGAAATCGTT GCCTTCGTTT 
GCCTCTACTG CAGCTGATAA AGAGAAAAGC CAAAATCATG AAAAAAAATC TCTGAACAGT 
GATAATCAAA GTTTGCGACC TTTGGAAAAT GAAAATCAAA GTGTTTCTAG CACCCAAGGT 
AGCAACAGCC CGCTTGAGTT GATCAATAAC TTACCAGCCT CAGATGACTA TTCTTCTATT 
ACCAAGGCTT TGAAAACCAG ATTTTTAACG TTTTCACCCT TTTTATCAAA GCCTCTTCCA 
CGTTTATTAA ACCAAGTTAA TTATTACCAG TTTATTCGTA ACAAATTACT GAAAGATGTG 
AAGTTACATC CATACTCACC AACTGGCTCA TTCGTAATGA AGCAGTCCGA AAATGACAAT 
GTAGAAGAAA GTTACGAAGA GTCCTATAAA TTCTTTTGTA TTGATGAACG TTATCATTTT 
TTAGAAAAGC AGTGGACACT TTATTACTCT CATTCAAAAC TCATGTTTTC TCCATTTTCT 
TCTCAAAGGA TTATTTTGTT ATATTCCATT ATTAACAAGG AAACTTCTGT TCCTTGTATT 
GGCCCTGAAC GTTGTCTATT AGAATTTTAT CGAGAAACTG ATTGCACTCT TGGTCAGTAC 
ATAGAGGACT CATGCTTGAA TACCAATGTA AGTTGTGGAG GTGAGTATTG TAAAACGAAT 
GATATGCTGT GGCATTACAG GAGTTATGTT CATGGAAATA GTCGGATCAG CGTTTTTTTG 
GAATCTTTTT CGTGCCCTGT CCCTGGTTTG GAAGAAAAGA TAATTATGTG GAGTTACTGC 
AAATTCTGTA AAAAGAATAC ACACATTACT GTTATGTCTG AAGAAACATG GAAATATTCG 
TTTGGAAAAT ATCTTGAATT CATGTTCTAT AATTCTCAAA TAAGGGATCG CTTCGAATTC 
TGTGACCACA GTGTTATGGC ACAACATGTT CATTATTTCG GTTATTGCAA CATGGCTTTA 
CGATTTCAAA GAGACCTAAT AGAGATTTTT GAACTCTTCG TTCCATCGGT CACTTTACGA 
AATAATCCAT CATATATTAA AGAGCTGAAA GAAAAGGAGT ATAAACGTTT AAAAGGTGTC 
ATTGAAAAAT GCTTGTCTTC TGTTGCTTCA AGGATAAACC AAATAAAGTG CGATTGGGTT 
ACTGATCCTG AGAAATTTGA GTCTTGTACA TCTGAAATTT CAAAATTTCG CACTTTACTT 
TCAAGTGACT ATACTGAATT GTATTCTGAA TTTGATTCAA TTTATCTTAA TTCCTCAACT 
TCTGATTATT TATCACTAAA CTCAATCCTG CGCGTCCTCC AGGGTAAAAT GGTAAAATGG 
GAACAGCGCT TTCTTGACTA TCAACGGCTG TATCTTCCAT CTTACAAAGA GTTAAGTAAA 
ATAGCAGCCG CTCAAATTAA GAAAGTGTTT CTGGAACGTC CATTAAGCCA AACCCCTTTA 
GATTTGCCAG AGACACTTGA GAATACTCAA ATTGATATAT ATCCCTCCTT TAAAACTGAA 
TCCACTGATG ATCAACTCGA GAAAGTTACC CAAACTAATG TTGCTTCAAA CAAGCGTGTT 
GCTCCGTATG CAGATAGTAT GGCAAATGTT GGTTCTCCAG AATCTGATTG CTTTTCAGTA 
GCAACATCTT CCGATATTCC TAAAGCCAAC ATCGATTTTA CCAATGATAT TTCTACTCAG 
AACACTTTTC CAGCTTCTCC GGTCTCAAAT TCAGGTTTCA GTAGACAAAC GTATCCAAAT 
ATAAGCCAAA GACAAGGCGT TAATATGCTT TCTCATAAGC GGAAAAGTGC TAGTACCAGT 
GACAGGCGAT TTGTCAATGC ATCTTCAACA AGTGGGATGA ACATGCCTAT TTCTTCTTCA 
ATATCTGCAA AAATTTCCAG TATACAGAAT TCTACAAAAT ACTCACCCAG GAAGCCCATT 
CCAGCAAAAG ATGTGCGGGT TAGCTCTTTA GTGCGTAGAT TTGAAGAGCT AAGTTTACAA 
TTACAAGAAA AGCAAAAACG AGATGAAGAA TTAATAAAGG CTCGACGCAA GCGGGCTCTT 
CCTGTAGTCC CATCTAAGCC GGTAGTAGAA GTATTTAATG ACCTTAATGA AGCATTTGAT 
GATGAAAATT CCGAAGATGA GAATGGCATT AATGACACAA AAGAAAATCG CGCTACCGAA 
AGCAATTTTT CTGGAGTTGA TTCTATGAGC AAGGAGCGAG AAAATGTTTC TTCGAATGAA 
GACAATTCAC CTGAAGCGTT TGAAGATATT TTTGGAATTC TTTTCAAAAA TGAATCTGGC 
CTCGAAGAAC AGCAAAACCT TGAACCTTCT AGTCAAATGG ATAAAGAGGG ATCAAAGTTG 
CCAACTTCTG GTCCTTTGGC TGACAAAACT TCAGTTTACA GGATTTTATC TGCATTTTGG 
AATGAATGGA ACTCCCTCAA TCCTCCACCT TTTGAATTTC CTCTACAACC TACAGAGCAT 
ATGTTTTCCG ACTCAAATGT GATCATAAGG GAAGACGAAC CTAGCTCCTT GATAAGTTTT 
ACTCTTAGCT CTCCTGACTA TTTATCAAAA ATGGTAGAAA TTGAAGATAG CATGGACGAA 
GCTTTAACCA ATCAAGGCTT ACAAGGCTCG ACGCAATTCA AAATTGAAAA CTTAATGCTC 
AAACCAACGG GGACGCATTT GAAATACCAG TTTGAAGAAG GATCAGCAAG ACTATCCTGT 
AAGGTCTTTT TCGCGGAACA ATTTAGTGCT TTGCGAAGAG CTTGTGGCTG TGAGGAGACG 
TTTGTGACAT CTCTAGCGAG ATGTTCTCTT TGGGAATCTA GCGGTGGAAA ATCTGGATCA 
GCATTTTTAA AAACTTTCGA TAAGAAATAC ATTCTCAAAG TTCTGTCAAG GTTGGAAAGT 
GACTCCTTGC TTAATTTTGC TCCAGCTTAT TTTGATTACA TATCTAAAGT TTTCTTTCAC 
GAGTTGCCAA CTGCATTGAC TAAAATCTTT GGATTTTACC GCGTAGACAT TCGAAATCCT 
ACTACTGGTA CAATCTGTAA GACTGATATA ATGATTATGG AAAATGTATT TTATGATGAA 
TGTCCGTCTA GAATATTTGA TCTTAAAGGT TCCATGAGGA ATCGACATGT AGAATCTACA 
GGGAAGGTAG ATGAAGTATT GCTTGATGAA AATTTGGTTG AACTTATTTA CGAATCTCCA 
ATATTTGTTA GTGAACAACT AAAAAGTCTT TTGCATTCTT GCCTTTGGAA TGATACATTG 
TTCCTCTCAA AACTCAATAT CATGGATTAC TCTTTAATTG TTGGTATTGA TTATACAAAA 
AAAGAACTTT ATGTTGGAAT AATTGATTTC ATACGAACAT ACACTTGGGA CAAAAAACTT 
GAAAGTTGGG TAAAGGAGAA AGGCTTAGTT GGTAGAGGTC CTGAACCAAC AATTGTTACT 
CCAAAGCAAT ACAAAAATAG ATTCCGAAAG GCTATGGATT GCTATATTTT AGCATCTCAG 
GATTTTGAGA CTGGGGAAGG TTTTAAGTTT TGCGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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fab1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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