stt4 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	stt4
Entrez Gene ID	2540883
Full Name	1-phosphatidylinositol 4-kinase Stt4 (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021211.2	NP_595304.1	putative 1-phosphatidylinositol 4-kinase Stt4

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	stt4	NP_595304.1
Saccharomyces cerevisiae (baker's yeast)	STT4	NP_013408.1
Magnaporthe oryzae (rice blast fungus)	MGG_13790	XP_003710881.1
Kluyveromyces lactis	KLLA0C17292g	XP_452973.1
Eremothecium gossypii	AGOS_ADL033W	NP_984064.1
Neurospora crassa	NCU09367	XP_963777.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following stt4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the stt4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101478	NM_001021211.2 Latest version!	Schizosaccharomyces pombe putative 1-phosphatidylinositol 4-kinase Stt4 (stt4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

stt4 ( NM_001021211.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595304.1 Schizosaccharomyces pombe putative 1-phosphatidylinositol 4-kinase Stt4 (stt4), mRNA.

Price & Availability↑

CloneID	OSc101478
Clone ID Related Accession (Same CDS sequence)	NM_001021211.2
Accession Version	NM_001021211.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5634bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative 1-phosphatidylinositol 4-kinase Stt4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021211.1.

ATGGATTGCA TCGATAAATC AATCAGAAGG GAATCTTTAC GAAAACTTGG CTGCCTTCCC 
GATCACGGTT GGAATCTGTT CGTACAATCA AGTAATCGTT ACTCAAAATT GCTTGAAGCA 
GATTCTAAGC TATTTAAGGC CCAAAGAATT GAGGACACTG TCTTGTCAAT TTGTGAAGTT 
ACTTTGGAAA ACAAAGCCTC TGAAAATGCC TTGACTTTGT TCCAACAGCT TCGCTTTTAC 
TTGCTGAATT ATCTATTTTT GGAAGATACC TCGGAGTACA AAAGTGTTTT ATGCTCACTT 
GATGAATCTG TCTATCCTAA TCTTTTACCA CTGACTGCGA ATATTTGCCC ATGTATTCTT 
AAGGCGCTTT TAGCGCTTGT CAGGCAGCAT AGAGAATTAT CTTCCGATAT AGTGAAGTTT 
TTGAATAGCA TTTTTTTGGC ACTAACTCGT ATTCTAAATA CTCCTCATTC GTCAAATAAT 
AATCCAACTC ATACTAAAAC GACAATCTTT GAGAAAGTGT TTTATGCCTC CCACCTTGGT 
TTCTTTTTTG AAGAAATAGC CGATGCTTGC ATATCAGCTT TGCCGCAAGA CGCGTGTTTA 
TGGGCAATGG AGGTTTGTAA CACGATACTC GTCTCAGACC TTCCGTGTAA ATTAATAGAC 
CTTCATAATG ATTCTTTTCT TCACCATTCC AATTACTATG CTGGGTTTGG GTTGAGCTAT 
TACCTTTACC TTTCATCTAT GAAAGCATAC ATCAATTCTT CTGGTTGCCT TTGGTATGAA 
GATGCTACCT CATTTTGGGA TATTGTGAAA CATCGCCCTT CTTCTCCGGA GGAGAAGCCG 
TTTTTAACGC AATTCCAAAG CATCTTAAAC CTTAGTGATA AGTTTTTGTC AAAAACTGGG 
ACCTTGAGAC TTCAAGAGAA GTTGGGTACT TCATGCAATT TATTTTTTGT ACTCGTACTA 
AAATTGGCAC TTCTATCCTC CTCCTACATT GGTTTTGTTC CATACACATA TAGTGATTGG 
ATTAATGTGT TGTTAGGTTC CATATCTGAA GATCCGGAGC TCTTACCTCA TTTAATTGAA 
CTTATGGCTT CGTTGGCCTG TGTTTTCCCC ACTCATTTTC AGTTTGCTAT GAAAAGATTG 
CGTTTAATCG CTATTTATGC CCCCCGCTTT GATGATGGTA ATGTGACTTA TGCTCAATTA 
GCCTCTAGAA AGCTTGCTCA ACTTTTCAAC TATTCCTCAA GAGATACTGC TATCACTTCA 
ATATACCAGT TTGCCAACTT GCTGTCTCCT GCTGACACTC CTGATAGTTA TCTTGCAGCT 
CCAAAAGAAC GCCTTTCTAT TAGTGACAAT ATGTCTAGTT CTTCTTCACA AACTGCAACG 
GTAAATACAA TTTCAAACTA TTTGAATGTT ATAGATTCTG TTCGTGAAAT TGCTTTGACA 
GTTAACGATG AGAAAATTTA TGGATTAGCT ATTTCTTTGT TGATACAAAA GTTCTCAAGA 
AAATTCGACT CGAGGGTGAG TACTGAATTA GTGAGAACAC TAGCAGATAT ATCCACTAAA 
GCTAATGATC GTGACTTTTC AATATTGGTT CAGTTTTATT CTATTCAAAA CAAAATGCTC 
GTGAAGAACG GTGATGAAGC TCTGACAATT GCTATTTGTG ATTCCCGCTG TTTGATCGCT 
AGAGGTACGG AATCTAACTC TTTCAAGAGG TCAGATTTGT TGAAAAGTCT TCTAGAGGAG 
ATTATCCATA TAGGTATCGT TCGTGATACA CCTGCCCCTG AACTGAAAGG ATATTATTTT 
GGCATGTCTA AAGCCGTCCA GGCTTTAGTC GAGTGTGTTG CAGGTACTGA TTGGAGCAGT 
TTGCCCAAGG AAGAAATGTA TCCTGCACTG TTCCGTGACA TGTGGTTTAC TTTAGTCATA 
AATCGTTTCC GTTATTCAGT TGATTTAGGT GAAACACTAG AAGTTGACCT TGAAAATATT 
GCCAAAAATT CTCCCCTTCT TGTTTTTGAG GATTTTGGTA GCAATTTTGA GAGCAATTTG 
GAACTAAACA CAGTCCTCAG GACTCATATT GAGCATTCAG TCATTTCTCA GATTAAATCA 
GAGTTGTTAG TCAGGGTCCC TAACATTGAT CTTAAGCCTT TGAGTACTTC AGAAATTTGC 
TTCTTGTCTG CTGTCCTTTT ACTTGAATCT TTGCGTTGCA AATCGAATAA GCTTTCTGCG 
CTTGTAGAAT ACTTGCTTGA TCCATCTTTG AGAGACAGTC AACTCCCCCA ATCTATTCGT 
GCTATTGCCC TATATAACTT AACATCTTTC GTTGAATCAT TGTCCAAAGA AAGAAGAGTT 
GCTTCTTCTA CTATCGACGA ATTTCAAAAA CTGTTGTGTC TTTGTTGTAA TAGAGTTGAT 
TGTGTACGAC AATTGGCTTT GGAATGTATG AATTATATTA TGGAGACGTT ACCTCATTTA 
CTCGGTATCA AAGAAATACT GTTTAGTGTT TTAGAGCTTT CCAGCCTTCT TTGGAAAGCT 
CGCACTGAAG AATGCACTGA TCAGTATGTC CCTCAATTAT TATACAAATC CAATAAGCTT 
AATTTGAGTG TAATACTTTC AGATATTTAC TCTTGCAGGG AAGAAGTTCT ATTTCAATTT 
AATAGACATG CTAGATCATG GATTCAAAAT TCTTCCAAAG CAATTCCTTA CCAGGTTAAG 
AACTTGCTTG AATCTTATTT AGCAGACTTT GTGGACTTTG ACGATTTAGA AGTCGTTGAG 
CTTGGACGAT CTCTTGCTGT TGAGCTTGGC ACTCGAATCG TGAGTTCTGA TCGTGACAAT 
TTTGTTTTGC CTGCTTTTGG AAATTGGACT CCCGATACGA GCTCGGAATT TATGGCCGAA 
TATACCATTC GTCAACGGTA TTCTCATGTC GACAATTCTA TTTTGAATAT TGAGGGGGAT 
ATGTCAACAG ATAGAATAGA TCTTATTTTG AACGAAAAAT CCAAGCTTTT TGAAACTCAT 
TTAGCAGCAC TTAAAAGTTT AGAGGATGAT ATATTACAGG GAAATACTGT TTCTCCGCAA 
AGACTTCGTA ACGAGGTTAG AAAAGCAGCC GCGCATGCTG TTCAAGAACC TATATTTCAA 
TTCTCCGTTT TGGCTCGAAA AATTGTTCGT ATTCCATTTC TTGATTTTTC ACCTTCTTCG 
TTCAAACTCG GGATCACTTT ATGGAATTGG ATGATGAACC AGGTTCCCTC ATTCAGTTCT 
TTTCTGATTT CGAATATTAT TAGAAACTGG AAAAATGAAA TTGTAACCGA AAAAAGGGGT 
TACTTTTCGA CAGCCAAAAG TAAATCTCCG TTGGCACTTC CAATGACTTA TTCTCCGACT 
GAAAGAGCTT CGTTTTTGTC CTATAAAAAC AAGGTTATGT CTCAAATGAT ACCGCACTTA 
TTGTTGTTGC AACTAATTGC AGGAAATTTT GAGGGCTTCT GGTACGGTGA TAGACAGACT 
GCTAAACTTA TTGTACATTT CATGAAATTC GTACTTAAGA AAATCACTTC AATGGAAGTC 
AATCTGAACG TTTTAACTAG GGAGCTTCAT TTCAAATTTG TTTCATTTGG CCTTCGTATC 
GCTGAAAATT TGTTGAATAG CCCGCTTGGT TCAAGGTTTT ACAATCTTTG TGTTGATGCT 
GGACTATGCT GGTTCAGCGG AATGCCCAAC TGGACGTATG GAGCTGACAA ATTACATGTA 
GCAGCGGAAA TAAGTGTTAT GCGAAGTCTT CGCGATAAAT TAGACAGCTT TTTATTACGT 
TATCCACTTA AAGTGTCTAC CACATTAAAG CAAAAGCTTC TTATTATATT ACTCAATAAT 
GAAATGTATA TGCTTTATAC TTGGCTGACC CCTGTTTTGC ATGGTCGAAA TGTCAGGATG 
GTTGAACCTA TTCCTGATTC AGATGCCGCT TCGTCGCCAA TTACTTTGGA AATGTTACAG 
GTTGCTTGGA ACGTTTCTCC TAATATAGTG TTATACGCTC CTAAAAGATT TCAAAATGCT 
CCATTGAAGC AGATGGCGTT GAATTTCGTC ATTGCAAATC CCTTTACTAG TGTAAAACAT 
GAGGTTGCTT TGGAATATCT GTTTGAGCAC TATCCAAGCG GTGAATTTCC TATAGATCGC 
AAGTTTATTC TATACTGGGA GCCAATGTAT CCAGTTTCAG GGCCTGTAAT GTTCATGCCG 
AATGTGAAAT GGAATCCACA ATTATTGCAG TACACCATGC GTTCTATGGA AAGTTACCCA 
GTTTCTGTAA CGTTTTTTTA TGTTCCTCAA ATTGTTCAAT CATTACGTTA TGATAGCATG 
GGATATGCTG AAAGTTTCAT TCTCGAAACC TCAGGAACAT CACAGTTGTT TGCTCATCAA 
ATTTTGTGGA ACATGAAAGC TAATCTTTAT AAAGATGAAG CCGCTACTGT ACCTGATTCT 
ATAAAACCAA TATTGGATAG GGTAATGGAT AAAATGATTA ACTCTTTATC TGGAGAAGAT 
AAGCAATTTT ACGAACGAGA GTTTACCTTT TTCAATGAGG TCACGTCAAT TTCAGGAAAA 
TTAAAGCCGT TCATTAGAAA ATCTAAACCC GAAAAAAAGG CTAAAATTGA TGAGGAGATG 
AAGAAGATAA AACTTGACGT TGGTGTTTAT TTGCCAAGTA ATCCTGATGG TGTAATCGTA 
GGAATAGATA GAAAGTCGGG AAAGCCTCTT CAAAGTCATG CTAAAGCTCC TTTCATGGCA 
ACATTTAAAA TACGCAAAGA AAAGTTGGTT GATGCTGACC CTGAGGAATT GGCGGTGAAT 
GGTACAGAAG AAGAAGCCGG CGATTCGGCT AAGAAACAAA CTTATGAAGT TTGGCAATCG 
GCCATCTTCA AGGTTGGTGA TGATTGCAGA CAAGATGTCT TAACACTCCA ATTAATTGCA 
ATGTTCAAAA ATATTTTTAA CTCTGTTGGA TTAGACGTTT ATCTTTTTCC TTATCGAGTT 
ACTGCAACTA ATCCGGGCTG TGGTGTTATT GATGTTTTAC CAAATTGTAT TTCACGCGAT 
ATGCTAGGTC GGGAGGCTGT CAATGGATTG TATGACTACT TCCGCACAAA GTTTGGTGAT 
GAAGATTCTA TAGCATTCCA AAAAGCCCGT AGTAACTTCG TACAAAGTAT GGCCGCTTAT 
TCAGTTATTA CATACTTATT GCAATTTAAG GATAGACACA ATGGAAATAT CATGATTGAC 
GACCAAGGTC ATATTTTGCA TATTGATTTC GGTTTTATTT TTGACATTGC TCCTGGAGGT 
ATTACCTTTG AGAGTGCACC TTTTAAATTG ACAACAGAGA TGATCGCTGT TATGGGAGGC 
AGTAATAAAT CGCAGCCATT TCAGTGGTTT CAAGAGCTTT GTGTCAAAGC ATTCCTTGCC 
TGCAGACCTT ATGCCCATTA TATATGTCAA GCTGTGGAGG TTATGTTGGA TTCGGGTCTT 
CCTTGTTTTA AGGGGCAATT AACTATTACT CATTGCCTGG AGAGATTTGC TTTGAATTTA 
AATGAACGGC AAGCATCTAC ATTTATGCTT CATTTGATTG AGCAGAGTTA TGCAAACAAA 
CGTACGCTTA TGTACGATCA ATTCCAGAAG GCCACAAATG GTATTCCTTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595304.1
CDS	349..5982
Translation	MDCIDKSIRRESLRKLGCLPDHGWNLFVQSSNRYSKLLEADSKLFKAQRIEDTVLSICEVTLENKASENALTLFQQLRFYLLNYLFLEDTSEYKSVLCSLDESVYPNLLPLTANICPCILKALLALVRQHRELSSDIVKFLNSIFLALTRILNTPHSSNNNPTHTKTTIFEKVFYASHLGFFFEEIADACISALPQDACLWAMEVCNTILVSDLPCKLIDLHNDSFLHHSNYYAGFGLSYYLYLSSMKAYINSSGCLWYEDATSFWDIVKHRPSSPEEKPFLTQFQSILNLSDKFLSKTGTLRLQEKLGTSCNLFFVLVLKLALLSSSYIGFVPYTYSDWINVLLGSISEDPELLPHLIELMASLACVFPTHFQFAMKRLRLIAIYAPRFDDGNVTYAQLASRKLAQLFNYSSRDTAITSIYQFANLLSPADTPDSYLAAPKERLSISDNMSSSSSQTATVNTISNYLNVIDSVREIALTVNDEKIYGLAISLLIQKFSRKFDSRVSTELVRTLADISTKANDRDFSILVQFYSIQNKMLVKNGDEALTIAICDSRCLIARGTESNSFKRSDLLKSLLEEIIHIGIVRDTPAPELKGYYFGMSKAVQALVECVAGTDWSSLPKEEMYPALFRDMWFTLVINRFRYSVDLGETLEVDLENIAKNSPLLVFEDFGSNFESNLELNTVLRTHIEHSVISQIKSELLVRVPNIDLKPLSTSEICFLSAVLLLESLRCKSNKLSALVEYLLDPSLRDSQLPQSIRAIALYNLTSFVESLSKERRVASSTIDEFQKLLCLCCNRVDCVRQLALECMNYIMETLPHLLGIKEILFSVLELSSLLWKARTEECTDQYVPQLLYKSNKLNLSVILSDIYSCREEVLFQFNRHARSWIQNSSKAIPYQVKNLLESYLADFVDFDDLEVVELGRSLAVELGTRIVSSDRDNFVLPAFGNWTPDTSSEFMAEYTIRQRYSHVDNSILNIEGDMSTDRIDLILNEKSKLFETHLAALKSLEDDILQGNTVSPQRLRNEVRKAAAHAVQEPIFQFSVLARKIVRIPFLDFSPSSFKLGITLWNWMMNQVPSFSSFLISNIIRNWKNEIVTEKRGYFSTAKSKSPLALPMTYSPTERASFLSYKNKVMSQMIPHLLLLQLIAGNFEGFWYGDRQTAKLIVHFMKFVLKKITSMEVNLNVLTRELHFKFVSFGLRIAENLLNSPLGSRFYNLCVDAGLCWFSGMPNWTYGADKLHVAAEISVMRSLRDKLDSFLLRYPLKVSTTLKQKLLIILLNNEMYMLYTWLTPVLHGRNVRMVEPIPDSDAASSPITLEMLQVAWNVSPNIVLYAPKRFQNAPLKQMALNFVIANPFTSVKHEVALEYLFEHYPSGEFPIDRKFILYWEPMYPVSGPVMFMPNVKWNPQLLQYTMRSMESYPVSVTFFYVPQIVQSLRYDSMGYAESFILETSGTSQLFAHQILWNMKANLYKDEAATVPDSIKPILDRVMDKMINSLSGEDKQFYEREFTFFNEVTSISGKLKPFIRKSKPEKKAKIDEEMKKIKLDVGVYLPSNPDGVIVGIDRKSGKPLQSHAKAPFMATFKIRKEKLVDADPEELAVNGTEEEAGDSAKKQTYEVWQSAIFKVGDDCRQDVLTLQLIAMFKNIFNSVGLDVYLFPYRVTATNPGCGVIDVLPNCISRDMLGREAVNGLYDYFRTKFGDEDSIAFQKARSNFVQSMAAYSVITYLLQFKDRHNGNIMIDDQGHILHIDFGFIFDIAPGGITFESAPFKLTTEMIAVMGGSNKSQPFQWFQELCVKAFLACRPYAHYICQAVEVMLDSGLPCFKGQLTITHCLERFALNLNERQASTFMLHLIEQSYANKRTLMYDQFQKATNGIPY

Target ORF information:

RefSeq Version	NM_001021211.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative 1-phosphatidylinositol 4-kinase Stt4 (stt4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021211.2

ORF Insert Sequence:

ATGGATTGCA TCGATAAATC AATCAGAAGG GAATCTTTAC GAAAACTTGG CTGCCTTCCC 
GATCACGGTT GGAATCTGTT CGTACAATCA AGTAATCGTT ACTCAAAATT GCTTGAAGCA 
GATTCTAAGC TATTTAAGGC CCAAAGAATT GAGGACACTG TCTTGTCAAT TTGTGAAGTT 
ACTTTGGAAA ACAAAGCCTC TGAAAATGCC TTGACTTTGT TCCAACAGCT TCGCTTTTAC 
TTGCTGAATT ATCTATTTTT GGAAGATACC TCGGAGTACA AAAGTGTTTT ATGCTCACTT 
GATGAATCTG TCTATCCTAA TCTTTTACCA CTGACTGCGA ATATTTGCCC ATGTATTCTT 
AAGGCGCTTT TAGCGCTTGT CAGGCAGCAT AGAGAATTAT CTTCCGATAT AGTGAAGTTT 
TTGAATAGCA TTTTTTTGGC ACTAACTCGT ATTCTAAATA CTCCTCATTC GTCAAATAAT 
AATCCAACTC ATACTAAAAC GACAATCTTT GAGAAAGTGT TTTATGCCTC CCACCTTGGT 
TTCTTTTTTG AAGAAATAGC CGATGCTTGC ATATCAGCTT TGCCGCAAGA CGCGTGTTTA 
TGGGCAATGG AGGTTTGTAA CACGATACTC GTCTCAGACC TTCCGTGTAA ATTAATAGAC 
CTTCATAATG ATTCTTTTCT TCACCATTCC AATTACTATG CTGGGTTTGG GTTGAGCTAT 
TACCTTTACC TTTCATCTAT GAAAGCATAC ATCAATTCTT CTGGTTGCCT TTGGTATGAA 
GATGCTACCT CATTTTGGGA TATTGTGAAA CATCGCCCTT CTTCTCCGGA GGAGAAGCCG 
TTTTTAACGC AATTCCAAAG CATCTTAAAC CTTAGTGATA AGTTTTTGTC AAAAACTGGG 
ACCTTGAGAC TTCAAGAGAA GTTGGGTACT TCATGCAATT TATTTTTTGT ACTCGTACTA 
AAATTGGCAC TTCTATCCTC CTCCTACATT GGTTTTGTTC CATACACATA TAGTGATTGG 
ATTAATGTGT TGTTAGGTTC CATATCTGAA GATCCGGAGC TCTTACCTCA TTTAATTGAA 
CTTATGGCTT CGTTGGCCTG TGTTTTCCCC ACTCATTTTC AGTTTGCTAT GAAAAGATTG 
CGTTTAATCG CTATTTATGC CCCCCGCTTT GATGATGGTA ATGTGACTTA TGCTCAATTA 
GCCTCTAGAA AGCTTGCTCA ACTTTTCAAC TATTCCTCAA GAGATACTGC TATCACTTCA 
ATATACCAGT TTGCCAACTT GCTGTCTCCT GCTGACACTC CTGATAGTTA TCTTGCAGCT 
CCAAAAGAAC GCCTTTCTAT TAGTGACAAT ATGTCTAGTT CTTCTTCACA AACTGCAACG 
GTAAATACAA TTTCAAACTA TTTGAATGTT ATAGATTCTG TTCGTGAAAT TGCTTTGACA 
GTTAACGATG AGAAAATTTA TGGATTAGCT ATTTCTTTGT TGATACAAAA GTTCTCAAGA 
AAATTCGACT CGAGGGTGAG TACTGAATTA GTGAGAACAC TAGCAGATAT ATCCACTAAA 
GCTAATGATC GTGACTTTTC AATATTGGTT CAGTTTTATT CTATTCAAAA CAAAATGCTC 
GTGAAGAACG GTGATGAAGC TCTGACAATT GCTATTTGTG ATTCCCGCTG TTTGATCGCT 
AGAGGTACGG AATCTAACTC TTTCAAGAGG TCAGATTTGT TGAAAAGTCT TCTAGAGGAG 
ATTATCCATA TAGGTATCGT TCGTGATACA CCTGCCCCTG AACTGAAAGG ATATTATTTT 
GGCATGTCTA AAGCCGTCCA GGCTTTAGTC GAGTGTGTTG CAGGTACTGA TTGGAGCAGT 
TTGCCCAAGG AAGAAATGTA TCCTGCACTG TTCCGTGACA TGTGGTTTAC TTTAGTCATA 
AATCGTTTCC GTTATTCAGT TGATTTAGGT GAAACACTAG AAGTTGACCT TGAAAATATT 
GCCAAAAATT CTCCCCTTCT TGTTTTTGAG GATTTTGGTA GCAATTTTGA GAGCAATTTG 
GAACTAAACA CAGTCCTCAG GACTCATATT GAGCATTCAG TCATTTCTCA GATTAAATCA 
GAGTTGTTAG TCAGGGTCCC TAACATTGAT CTTAAGCCTT TGAGTACTTC AGAAATTTGC 
TTCTTGTCTG CTGTCCTTTT ACTTGAATCT TTGCGTTGCA AATCGAATAA GCTTTCTGCG 
CTTGTAGAAT ACTTGCTTGA TCCATCTTTG AGAGACAGTC AACTCCCCCA ATCTATTCGT 
GCTATTGCCC TATATAACTT AACATCTTTC GTTGAATCAT TGTCCAAAGA AAGAAGAGTT 
GCTTCTTCTA CTATCGACGA ATTTCAAAAA CTGTTGTGTC TTTGTTGTAA TAGAGTTGAT 
TGTGTACGAC AATTGGCTTT GGAATGTATG AATTATATTA TGGAGACGTT ACCTCATTTA 
CTCGGTATCA AAGAAATACT GTTTAGTGTT TTAGAGCTTT CCAGCCTTCT TTGGAAAGCT 
CGCACTGAAG AATGCACTGA TCAGTATGTC CCTCAATTAT TATACAAATC CAATAAGCTT 
AATTTGAGTG TAATACTTTC AGATATTTAC TCTTGCAGGG AAGAAGTTCT ATTTCAATTT 
AATAGACATG CTAGATCATG GATTCAAAAT TCTTCCAAAG CAATTCCTTA CCAGGTTAAG 
AACTTGCTTG AATCTTATTT AGCAGACTTT GTGGACTTTG ACGATTTAGA AGTCGTTGAG 
CTTGGACGAT CTCTTGCTGT TGAGCTTGGC ACTCGAATCG TGAGTTCTGA TCGTGACAAT 
TTTGTTTTGC CTGCTTTTGG AAATTGGACT CCCGATACGA GCTCGGAATT TATGGCCGAA 
TATACCATTC GTCAACGGTA TTCTCATGTC GACAATTCTA TTTTGAATAT TGAGGGGGAT 
ATGTCAACAG ATAGAATAGA TCTTATTTTG AACGAAAAAT CCAAGCTTTT TGAAACTCAT 
TTAGCAGCAC TTAAAAGTTT AGAGGATGAT ATATTACAGG GAAATACTGT TTCTCCGCAA 
AGACTTCGTA ACGAGGTTAG AAAAGCAGCC GCGCATGCTG TTCAAGAACC TATATTTCAA 
TTCTCCGTTT TGGCTCGAAA AATTGTTCGT ATTCCATTTC TTGATTTTTC ACCTTCTTCG 
TTCAAACTCG GGATCACTTT ATGGAATTGG ATGATGAACC AGGTTCCCTC ATTCAGTTCT 
TTTCTGATTT CGAATATTAT TAGAAACTGG AAAAATGAAA TTGTAACCGA AAAAAGGGGT 
TACTTTTCGA CAGCCAAAAG TAAATCTCCG TTGGCACTTC CAATGACTTA TTCTCCGACT 
GAAAGAGCTT CGTTTTTGTC CTATAAAAAC AAGGTTATGT CTCAAATGAT ACCGCACTTA 
TTGTTGTTGC AACTAATTGC AGGAAATTTT GAGGGCTTCT GGTACGGTGA TAGACAGACT 
GCTAAACTTA TTGTACATTT CATGAAATTC GTACTTAAGA AAATCACTTC AATGGAAGTC 
AATCTGAACG TTTTAACTAG GGAGCTTCAT TTCAAATTTG TTTCATTTGG CCTTCGTATC 
GCTGAAAATT TGTTGAATAG CCCGCTTGGT TCAAGGTTTT ACAATCTTTG TGTTGATGCT 
GGACTATGCT GGTTCAGCGG AATGCCCAAC TGGACGTATG GAGCTGACAA ATTACATGTA 
GCAGCGGAAA TAAGTGTTAT GCGAAGTCTT CGCGATAAAT TAGACAGCTT TTTATTACGT 
TATCCACTTA AAGTGTCTAC CACATTAAAG CAAAAGCTTC TTATTATATT ACTCAATAAT 
GAAATGTATA TGCTTTATAC TTGGCTGACC CCTGTTTTGC ATGGTCGAAA TGTCAGGATG 
GTTGAACCTA TTCCTGATTC AGATGCCGCT TCGTCGCCAA TTACTTTGGA AATGTTACAG 
GTTGCTTGGA ACGTTTCTCC TAATATAGTG TTATACGCTC CTAAAAGATT TCAAAATGCT 
CCATTGAAGC AGATGGCGTT GAATTTCGTC ATTGCAAATC CCTTTACTAG TGTAAAACAT 
GAGGTTGCTT TGGAATATCT GTTTGAGCAC TATCCAAGCG GTGAATTTCC TATAGATCGC 
AAGTTTATTC TATACTGGGA GCCAATGTAT CCAGTTTCAG GGCCTGTAAT GTTCATGCCG 
AATGTGAAAT GGAATCCACA ATTATTGCAG TACACCATGC GTTCTATGGA AAGTTACCCA 
GTTTCTGTAA CGTTTTTTTA TGTTCCTCAA ATTGTTCAAT CATTACGTTA TGATAGCATG 
GGATATGCTG AAAGTTTCAT TCTCGAAACC TCAGGAACAT CACAGTTGTT TGCTCATCAA 
ATTTTGTGGA ACATGAAAGC TAATCTTTAT AAAGATGAAG CCGCTACTGT ACCTGATTCT 
ATAAAACCAA TATTGGATAG GGTAATGGAT AAAATGATTA ACTCTTTATC TGGAGAAGAT 
AAGCAATTTT ACGAACGAGA GTTTACCTTT TTCAATGAGG TCACGTCAAT TTCAGGAAAA 
TTAAAGCCGT TCATTAGAAA ATCTAAACCC GAAAAAAAGG CTAAAATTGA TGAGGAGATG 
AAGAAGATAA AACTTGACGT TGGTGTTTAT TTGCCAAGTA ATCCTGATGG TGTAATCGTA 
GGAATAGATA GAAAGTCGGG AAAGCCTCTT CAAAGTCATG CTAAAGCTCC TTTCATGGCA 
ACATTTAAAA TACGCAAAGA AAAGTTGGTT GATGCTGACC CTGAGGAATT GGCGGTGAAT 
GGTACAGAAG AAGAAGCCGG CGATTCGGCT AAGAAACAAA CTTATGAAGT TTGGCAATCG 
GCCATCTTCA AGGTTGGTGA TGATTGCAGA CAAGATGTCT TAACACTCCA ATTAATTGCA 
ATGTTCAAAA ATATTTTTAA CTCTGTTGGA TTAGACGTTT ATCTTTTTCC TTATCGAGTT 
ACTGCAACTA ATCCGGGCTG TGGTGTTATT GATGTTTTAC CAAATTGTAT TTCACGCGAT 
ATGCTAGGTC GGGAGGCTGT CAATGGATTG TATGACTACT TCCGCACAAA GTTTGGTGAT 
GAAGATTCTA TAGCATTCCA AAAAGCCCGT AGTAACTTCG TACAAAGTAT GGCCGCTTAT 
TCAGTTATTA CATACTTATT GCAATTTAAG GATAGACACA ATGGAAATAT CATGATTGAC 
GACCAAGGTC ATATTTTGCA TATTGATTTC GGTTTTATTT TTGACATTGC TCCTGGAGGT 
ATTACCTTTG AGAGTGCACC TTTTAAATTG ACAACAGAGA TGATCGCTGT TATGGGAGGC 
AGTAATAAAT CGCAGCCATT TCAGTGGTTT CAAGAGCTTT GTGTCAAAGC ATTCCTTGCC 
TGCAGACCTT ATGCCCATTA TATATGTCAA GCTGTGGAGG TTATGTTGGA TTCGGGTCTT 
CCTTGTTTTA AGGGGCAATT AACTATTACT CATTGCCTGG AGAGATTTGC TTTGAATTTA 
AATGAACGGC AAGCATCTAC ATTTATGCTT CATTTGATTG AGCAGAGTTA TGCAAACAAA 
CGTACGCTTA TGTACGATCA ATTCCAGAAG GCCACAAATG GTATTCCTTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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stt4 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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