utp20 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	utp20
Entrez Gene ID	2540842
Full Name	U3 snoRNP protein Utp20 (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022638.2	NP_596713.1	putative U3 snoRNP protein Utp20

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	UTP20	XP_532665.2
Rattus norvegicus (Norway rat)	Utp20	NP_001178708.1
Gallus gallus (chicken)	UTP20	XP_004937716.1
Pan troglodytes (chimpanzee)	UTP20	XP_003313968.1
Bos taurus (cattle)	UTP20	XP_003586139.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005033	XP_313900.4
Caenorhabditis elegans (roundworm)	tag-184	NP_495323.1
Kluyveromyces lactis	KLLA0E17425g	XP_454735.1
Eremothecium gossypii	AGOS_AEL006W	NP_984855.2
Xenopus tropicalis (tropical clawed frog)	utp20	XP_004913232.1
Homo sapiens (human)	UTP20	NP_055318.2
	UTP20	XP_001091709.1
Mus musculus (house mouse)	Utp20	NP_780367.2
Danio rerio (zebrafish)	im:7159098	XP_001920500.3
Drosophila melanogaster (fruit fly)	CG4554	NP_611676.1
Schizosaccharomyces pombe (fission yeast)	utp20	NP_596713.1
Saccharomyces cerevisiae (baker's yeast)	UTP20	NP_009551.2
Magnaporthe oryzae (rice blast fungus)	MGG_07737	XP_003712944.1
Neurospora crassa	NCU02494	XP_965088.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following utp20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the utp20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100574	NM_001022638.2 Latest version!	Schizosaccharomyces pombe putative U3 snoRNP protein Utp20 (utp20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

utp20 ( NM_001022638.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596713.1 Schizosaccharomyces pombe putative U3 snoRNP protein Utp20 (utp20), mRNA.

Price & Availability↑

CloneID	OSc100574
Clone ID Related Accession (Same CDS sequence)	NM_001022638.2
Accession Version	NM_001022638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative U3 snoRNP protein Utp20
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022638.1.

ATGAAAGCTG CGGCGCAGAG CAATTCCAAA AACTATGTTT TTTTGCCTTT TTCCAAGAGA 
GTTGAAAATT TAAAGATTGA TGTAGCTCAT AAAATCCCTA GAGCTGCTGA TTTAGAGGAT 
GAAGACGTTG AGTCTTACTT TATTTCATGT CTCAGGAAAT GGGAGGACTT GAATCTTTCA 
ACCCACTATG TAAACTTTCT TCGTAGTGTC ACTCCATACT CACAGTCTTT ACCTCAAATA 
GTATATCATC AAAAAACAAT TTTTGATCTT ATTGTACAGT ATGCTCGTGA AGGAGATTCA 
CTGTCTTTAC AGCCAATCTT AGAATTATTG ACACAGTTTG CTCGTGATCT GGCACTGGAG 
TTTGCAAGTT ATATTGATCA AACCTTAGAA CTACTCTGCA TTCTCGTTCA GAATAACGAG 
TTGGAAGTTG TTGACTGGTC TTTTCATGCT GCTGCTTATC TTTTTAAATA TCTTCGAAAA 
ATTCTTGCAC CCCGTTTAAT TCATACTTAT GATATTCTAT CCCCTCTATT AGGTAAGGAA 
AAGCAGAAGA GTCATGTTAC TCGTTTTACC GCTGAGGCTT TATCTTTTTT GTGTAAAACA 
GTTTCTTACG AGAATGCAAT TGAGTTTACT GGACATGTGT TGCTCGATCT AAACGATCAC 
TATACTCCTC AATATCACGA GGGAGTCGTC ATTCTCTTTT CAAGGATAAT ACAGGGCGTT 
GATACGAGTA TTCACTTTAA AGGAAAAGCT TTTTTTGAAA TCCTATTACG ATCTGAGATT 
TACACCTTGC CAAGGTCTAA AAGTGTGATA ATTCCTGTCT TAATTTCTAC AATTCATCAT 
TGTACTTCAG ATTCCTTTAA CGAATTAGAA AAGTTAATAA TTTCTAAAAT AACCGGTGAG 
CCATTACTAT ATATAAATTT ATTTAAAGCT ACGTTGGTTA CCAGAAAGGG CTCTCGCGTA 
TCTGATTATC CGTCTTTCTT TAAAGCCTAT TTGCAGATAC CCAACTTAGT CAGCTGGGAA 
AATATAGAGT CAGATCTTTC AGTTCTGATA TTTGAAATAT CTGCTCTTCT TTTTGTATAT 
CCCCAAATTT CTGATTTAAT GCCTCACACG GTACGCATTA TTTCTTTTTT ACAACAAGGT 
CCATTACACT TGTTTTTCTC TTTTGTTGAT GTTGTGAAAC AGCTAAATAT GCAACGGTAT 
GATTCCTTCA TTAAACCATC TCTTCCCAAA TTTATTTCTT CACTAGTGAG TGATGAATCT 
CAGAAGTCGT TGGCAATTTT GGAAAGCGTT TTAGACAACT CTGTGCCCGT TCCAGTTGAT 
GTGGCTTCAG TCTCTCTTAA CCACTTTGTA TATTGTATTG AGAGACTTCA AATTGTTTCC 
CCGTCTTACG AAGATATCAT CTCCCTATGG TCTTCTTTGA TGATTATTAT TTCATCTTCA 
TTAGGATCTG ATGAAATCTA TAAGGCTTGC TTGCTTTTTT TAAGAAAGTT AGAAGATGTT 
TCATCTGAAT CAGTTTTATT AGGAGATATT TGTGGAATTG TATTGCATTT GCTTCAACGC 
AAAGTTTCTC GTTTATTTCT GGACAGCTCT TATTACAAGC CAATTCTAGA TTCTTTGCTT 
GGCGTTTTTG GTTTTTTAGC TGACAGTAAA GTCTTTTTAG AAAGTATTCG CCCCTTTTTC 
GGTACTTCAT ATGACTTTTG CAGCTCGTAT ACTTCATTAA TGGATCGTTT AATTAATAAT 
TTAAGTCGGG GGTTGACATC CCTAAGAGCG GCTTCTATTG ATTTGATTAT TGCATTGTGC 
AAATCTCTTC AGAAAAACGA GGTCCTACAA TCTCTATCTC TAGTAAAAAA GTTATCAGAG 
CTGCCTTTTG ATCCTTCAAC CAGTCGTGAT GCATCGGTCT TGCTACGAAA TTTGTCTGCC 
AAATCATCCG CTTTAGAGAA GGACACTAGA AGAGTTGTAT TACATGCCTT ACTAGGGCTA 
ACTATTACTC GATTCACCCC GCTTTGGCCG GATTTGTCAA GGACAGCAGC TTCCATCGTC 
ACGAAGGAAG TGGAAGATGA GTTTTTAGCT ATCATTTATT CTTGGCTTTC ACTTCCTTCT 
CCTCCCTCTG GTTTGTTAGG ACCAACTGAT ACTGCCGTGT TTGTGAAACC AGATTTGTCA 
CTAGAAAAGC TACCTACCTT AGAAATTAAC ACATTCAATT GTCCTGCGAT TTCTTACTTC 
GAAACATCTT TCGATGAGTG CTTTTCTAAG TTCGCTTCGT CTGACAACTA TATAATTGGA 
AACTTACTAA AAACAAATCA AGAAGATCTT TCAAATCTTC CGACTTTTCG GTCTCAGGCT 
TTACGAGTAC TCAATGAGCT TCCGGAAATA GCATCCCGTA ACATAAATGT TTTAGACAAT 
TATCTTTTTA GTTTACAACA TGGTTTCGAT CTTAATACGG AATGGGCTCG TCCTGATGTC 
TATTTGCTCT TAGGACTTTA CTCGAAATTC ACTGGCATCA AAGAGTTTGT CAATCGAGAT 
GCTCGAAAGG AATTTTTTCT TTGGGCCCTT ACTATTAATG ACCCAAAGGT TCAGAAATTG 
GTTCTAGACA TAATTCTTCT ATATTCAGAA GAAGCAATTA CTACTTACGA AGAAAATCTT 
CGAAACTTGC TGGATGATAA AAAGTGTAGA GATGAATTAA TTACATTTCT ATTTGTGGAT 
TACGCAGATA GCAAAATTCA AGATATTCAT CGTCCGTTAC TTATGCCTGT TGTAATATCC 
ATTCTCTACG GAAAAATGGT GTCTAAGGGA TATGGAGGAC AAAAGAATCA AGCTGCCAGA 
CGATCAACAA TTTTATCTGC ACTTGGGAAT ATGCAAGTAG AAGATTTACA GATTTTAGTT 
GATATTATGT TACGACCCTA TAATGGTCTT GAGGTCAAAT TGAACGCTAA TAATAATTTG 
GAGATCGACA CTGGAAACAT TCCTTCTTTG ACACTACGGC GACAAATTGG TTTTCTGACA 
ATGACCGAGG AACTTTTATT GCAGATTTCT TCTAAACTTT CTTCAGTTGC TCCCAAAATT 
CTGAATGCCG TTCTCTATAA TTTGGTTGTA TCCGATGATC AGATTTCCTC TCAAGAGGCT 
TTCGAGAATT TTGAGGTGAA AATGGCATAT ACGGTCAGAC AGCTATCACT GAAAGTTTTT 
CTACTTTTTT TGAAGTCCTG CTCTGATGTC GATTTCAAGC CTTATAATGT TTTTATATAT 
ACTGCTTTTG TGGTTCCACG CCTTGATCGG TTTGCTGATG AAAACACACA AAGTGTGTCA 
AATCTAATGA AAATTTTCCG TTGCTGGTTT GAGAATGAAG CCTATTTAGA TAGCGTCCTT 
GAGTTTTCTT CTCATATATT AACTGCTTTG CTTAACACTG CGTCTCACAC TGCCGTCAAA 
TTGCCGGTGT TGCTATACAT TCTTGATACT TTGAACTTGG TCATAACTCA TTTACAATCA 
GAAGAAAGTG AATCGCAGTT AAGAGAAAAG ATCTTGGCAA ATTTGGTTAT GCCGAACATC 
ACACTTATTT TTGCGAGTCT TTCGAATATT TTGAAGAATC CCCAATTTTG CAATAATAAT 
CGTGTAATGG ATGGATCCGT TCAGGCATTA TCTGCTGTAT CAGAATATAT GTCATTAGAT 
ATTGACTCTT CGCCTTTACT TAATCTGTTG GTTTCATTTT TGAGGAAACC CAACCGTCTG 
GTCCCTTCAA ATGTTAAGTC TAATATTTTA GTTTTGCTTT GTAAACTTCT TCCAACCAAT 
ACGAAATGGC TGCATGCTTC AATATCACAG ACAAATGATT TTGATACTAT TATGCATCTA 
TATACTTCTA TGGTTGACAT CAAGGCTCGT CAACATTTGA ACGATCTTTT GAAGATTTAT 
TCAACAATTG ATGATAACCT TATTTTCAGC TCAGTATTTG TTGAAGAAAT AAATTCTATT 
TCTAAAAAAC GGCTTGATGA ACCAGATTTT GAGCGTCGCT TATCTGCTTT TACCTCATTT 
AATGAAAAAC ACTTTTCTTT GATTTCAGAT TTAGCTTGGC TGCCTGTGCT CTATAACTTC 
TTCTTTTACG TTCAAGACGC TGAAGAGCTG GCTATTCGCG CTAGTGCGTC CCTGGGGATT 
AAACGGTTCA TTGAATCAAT AACTATGAAT GATGCATCAA ATCAGTTTAA GATTGATGTC 
TTTGTTAAGT TTATTTTTCC CTTCATCAAA AATCAAATGA AAAATAAAAA TGAACTCATT 
CGGCAAGAGT TTATTGGCTT GCTTAGTTAT TCTATTAAAT CGTTAACGAT GGTCGATGCA 
ATTTCGGATA TGCAACCTTT GCTTTACGAA GGTGATGAAG AAGCCAATTT TTTCAATAAC 
ATTTTGCATA TTCAGTTGCA CAGACGTAAA CGAGCAATGA AAAGGCTTGT TAATGTCTGT 
GCCATTGGTG TGATTAGAAG TGGTAATATT TCTCAAATAT TTCTACCCTT GCTGGAGAAC 
TTTTGTTTAG GTAATGATAC CGTGCAAACT TTGTTAGATG AATCAGTGAT CACAATTGGA 
GAAATCATAA AGTGGGCACA TTGGAATCAA TACCAAGCTA TTTTGAAGCG ATATGTTTCG 
CTGTTAAAAA ATAACGCTAT TGACCAAAAA GTAGTTGTTC GTTTGATAAC GGCCGTTGTG 
TCAGCATTAA GGCCATTGGA TGATGCAGTC GCTTCCTATA CAAACTCCGA AATGAACATA 
GAACAGTTTG ATGGCCAAAA GAAGAAATGC GTTTTAGCCA GTAGCCTACC TTCCGAAGAG 
AGATTTACAG AAGTCCTTAC TAATGATTTT TTTCCTACCC TGATGTTATA TCTACATATA 
AGAGACGAAT CAACGGTTAC CCTTCGTGTT GCAATTGCGC TTTCAATCGT GCAACTTGTT 
GCGCTTCTTC CCGAGGAAGA AATTGTTCTA CGGTTAACTC CTGTTTTGAT AGATACTTGC 
CATATTTTAC GCAGTCGCTC TTTAGAGTCG CGTGATGCAA CCCGGAAAGC TTTAGCAGCG 
ATATCAAAAT TTCTTGGCCC GAAGTACTTT TCCTTTATTA TCAGTCAATT ACAAACTTCT 
TTGAAAAGAG GGTATCAACT CCATGTTCTA GGTTACACAG TTCATTACCT CTTATTGGCG 
ATTGAGGACG TGTATCCGTA CGGCTCTATA GATTACTGTA TGGATTCTCT GGCTCAAATA 
TTTGTTGATG AGATATTTGG AGAAGTTGGT GTTGAAAAAG ATTCTGAAGA TTACAAATCA 
AATGTGAAGG AAATCAAAGG CAATAAGAGC TACGATTCTT ATGAGATTGT TGCTAGAATT 
TCTAGCTTCG ATAGTTTGAG TACATTACTT CGTCCGGTGA AGAATGTTTT GTTCGAAACA 
AATGTTCCTA AATCTCTACG AAAAGTAGAT GAATTGTGTC GGAGACTTTC TTTAGGAATT 
GTCGCGAACA AACAAAGTGC TTCGCAGAGT TCTTTGATTT TCTGTTATAA TGTTTACGAA 
TTTGTTGTCA AGGAAAAGGA AACTGTTGCG GCATTGAAAC AGCAAGAAAA CGATGGTTAT 
CGGTCAGCTC CTAATTTCTT TTTAGAAAAC TCAAAGAAGT TAATAAGGTT TACTTTCGAT 
GTTTTACGTG GTGTATCTAA CAAACATAAA GAATTGTTGA CAGCCAGAAA CATGGCGGCT 
TTCGTGCCTC TAATCGGTGA ATCGCTTTTA AGTAGCTCTG AAGAAGTTCA AATTTCTGCA 
TTACGCTTTC TCGTTTTGCT TCTCCCTTTA AAAATTGATC AGGTTTTTTC GGGTAGTTCA 
GTCTTCACGT CTCAGGCTGT GAAATATATA CAGAATTCGC CTTCTACAAA CACAGAATTA 
TGTCAAGCAA GTTTTAAGTT TTTAGCATCT ATTTTACCTT ATGAAAATGT AAAGATTAAG 
GAATCGACTA TAAACTATTT ACTGGAAAGG GTTGGAACTG ATATTCAAGA ACCTGATCGT 
CAAGGTGTTA TGTTTTCGTT GGTTAGAGCG GTTATTGCTC GCAAAATCAT GACTCCTGAA 
CTGTACAAAA TAATTGATTT AATACGAGAT ATGATGGTGA CAAATCATAC TAAATCCACT 
CGTCAAACTT GTCGTCATTT GTATTATTCA TTTCTTCTTG ATTATCCCCA GGGTAAAACT 
CGACTATCCA AGCAAATATC ATTTATTCTT AAGAACTTGG AATATGAATT CGCACCCGGA 
CGCGAATCTG TAATGGAGCT CTTGCATCTT ATCTTGAATA ATTTTAGTGA TGCTTTATTG 
AAAGAGTATC ATCAAGGAAT ATTTATTGCT CTAGTTATGG TGCTAGCTAA TGACTCAGAA 
CCTCATTGTA GAGAGATGTC TGCAGAATTA ATAAAGCTCG TATATCAACG TGCTGACAAT 
GAGAATTTTA ATTTGATTCG ACAGTTGTTG AGTCATTGGA CTTCAGTTGA GAAAGCCGGG 
AAAAATTTGG TCCGTGTAAG TATGCAGCTG TTTGGTTTAT TATTTGAAAC TTTTGGCTTC 
GAGAGGATGG AAGAAGTGCA CCTTTTTACG AAAGTTTTTG AAAGGGTTCT TTCAACAACT 
ATTAGCCATC CAGAAGAAGC AACAAATGAA TGGGAGTTAA ACTATTTTGG TCTCCAATCT 
TGGTTGAAGC TGGTCTTGGC GGATCCCAAA AAGTCATGTG AAAAGGAGTT TTCAAAGATT 
TGGGAATCAA TGCGTTATCT TATACTTTTT AAGCATGCAT GGGTTCGACT TTCTGTCTCC 
CGTCTCTTTG GTCACTTTTT TGCAATAATC GGAGACTCAA ATTTTGGAAA GCTTTCATTA 
GGAATTGACG GAGTTGTTTT CAGTTTAGAC TTTGTAACGC AAATATCAAA TGCTTTACAA 
GCACAATTAC GTAGTCCGGT TCTTTCCGAA GAGTTGGGCA TGCAGGTTGC TAAAAACTTA 
ATATTCCTTA CTCGCTGGTT TAATAGCATT CGCAGTTCTG ATGATTCTCC TTTTTTGGAA 
ATATTTAGAA GAATGCGCAA AACCCTCAAG AAGCAAACTA TTGAAGAATA TAGCATCAAC 
AAGAAATACT TGATGCAATG GTTTGCTAGC GTTATCCATG TTTTCTCTGG TGAAGAATTG 
CAACCGGTCC TTTCTGAAAT TATTGCGGCT TTATATCGCT ATACAGAACT ACAGGAGGCT 
GAAAGGAAAT CGCAACAGGA ACTTGCAGAT TTAGTGACTG AGTCATTACA AGTGCTTCAG 
GAAAAAGTTG GCGCGACTGT GTTTGCTAGG GCTTATCAAG AAGTACGAAA TGCTGCTATC 
GAAGTTCGCA GAGAAAGACG TGAGAAACGA GCTATTGAGC AAGTTGTAGC TCCTGAAGTT 
GCTTCTCGCA AGAAAATTCG AAAGAATGAA AGAAAACGAG AAAATAGGAA ACAAAAAACA 
AACCATCATC GCATGGTAAA TTCAATTTTT AAAAATAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596713.1
CDS	48..7529
Translation	MKAAAQSNSKNYVFLPFSKRVENLKIDVAHKIPRAADLEDEDVESYFISCLRKWEDLNLSTHYVNFLRSVTPYSQSLPQIVYHQKTIFDLIVQYAREGDSLSLQPILELLTQFARDLALEFASYIDQTLELLCILVQNNELEVVDWSFHAAAYLFKYLRKILAPRLIHTYDILSPLLGKEKQKSHVTRFTAEALSFLCKTVSYENAIEFTGHVLLDLNDHYTPQYHEGVVILFSRIIQGVDTSIHFKGKAFFEILLRSEIYTLPRSKSVIIPVLISTIHHCTSDSFNELEKLIISKITGEPLLYINLFKATLVTRKGSRVSDYPSFFKAYLQIPNLVSWENIESDLSVLIFEISALLFVYPQISDLMPHTVRIISFLQQGPLHLFFSFVDVVKQLNMQRYDSFIKPSLPKFISSLVSDESQKSLAILESVLDNSVPVPVDVASVSLNHFVYCIERLQIVSPSYEDIISLWSSLMIIISSSLGSDEIYKACLLFLRKLEDVSSESVLLGDICGIVLHLLQRKVSRLFLDSSYYKPILDSLLGVFGFLADSKVFLESIRPFFGTSYDFCSSYTSLMDRLINNLSRGLTSLRAASIDLIIALCKSLQKNEVLQSLSLVKKLSELPFDPSTSRDASVLLRNLSAKSSALEKDTRRVVLHALLGLTITRFTPLWPDLSRTAASIVTKEVEDEFLAIIYSWLSLPSPPSGLLGPTDTAVFVKPDLSLEKLPTLEINTFNCPAISYFETSFDECFSKFASSDNYIIGNLLKTNQEDLSNLPTFRSQALRVLNELPEIASRNINVLDNYLFSLQHGFDLNTEWARPDVYLLLGLYSKFTGIKEFVNRDARKEFFLWALTINDPKVQKLVLDIILLYSEEAITTYEENLRNLLDDKKCRDELITFLFVDYADSKIQDIHRPLLMPVVISILYGKMVSKGYGGQKNQAARRSTILSALGNMQVEDLQILVDIMLRPYNGLEVKLNANNNLEIDTGNIPSLTLRRQIGFLTMTEELLLQISSKLSSVAPKILNAVLYNLVVSDDQISSQEAFENFEVKMAYTVRQLSLKVFLLFLKSCSDVDFKPYNVFIYTAFVVPRLDRFADENTQSVSNLMKIFRCWFENEAYLDSVLEFSSHILTALLNTASHTAVKLPVLLYILDTLNLVITHLQSEESESQLREKILANLVMPNITLIFASLSNILKNPQFCNNNRVMDGSVQALSAVSEYMSLDIDSSPLLNLLVSFLRKPNRLVPSNVKSNILVLLCKLLPTNTKWLHASISQTNDFDTIMHLYTSMVDIKARQHLNDLLKIYSTIDDNLIFSSVFVEEINSISKKRLDEPDFERRLSAFTSFNEKHFSLISDLAWLPVLYNFFFYVQDAEELAIRASASLGIKRFIESITMNDASNQFKIDVFVKFIFPFIKNQMKNKNELIRQEFIGLLSYSIKSLTMVDAISDMQPLLYEGDEEANFFNNILHIQLHRRKRAMKRLVNVCAIGVIRSGNISQIFLPLLENFCLGNDTVQTLLDESVITIGEIIKWAHWNQYQAILKRYVSLLKNNAIDQKVVVRLITAVVSALRPLDDAVASYTNSEMNIEQFDGQKKKCVLASSLPSEERFTEVLTNDFFPTLMLYLHIRDESTVTLRVAIALSIVQLVALLPEEEIVLRLTPVLIDTCHILRSRSLESRDATRKALAAISKFLGPKYFSFIISQLQTSLKRGYQLHVLGYTVHYLLLAIEDVYPYGSIDYCMDSLAQIFVDEIFGEVGVEKDSEDYKSNVKEIKGNKSYDSYEIVARISSFDSLSTLLRPVKNVLFETNVPKSLRKVDELCRRLSLGIVANKQSASQSSLIFCYNVYEFVVKEKETVAALKQQENDGYRSAPNFFLENSKKLIRFTFDVLRGVSNKHKELLTARNMAAFVPLIGESLLSSSEEVQISALRFLVLLLPLKIDQVFSGSSVFTSQAVKYIQNSPSTNTELCQASFKFLASILPYENVKIKESTINYLLERVGTDIQEPDRQGVMFSLVRAVIARKIMTPELYKIIDLIRDMMVTNHTKSTRQTCRHLYYSFLLDYPQGKTRLSKQISFILKNLEYEFAPGRESVMELLHLILNNFSDALLKEYHQGIFIALVMVLANDSEPHCREMSAELIKLVYQRADNENFNLIRQLLSHWTSVEKAGKNLVRVSMQLFGLLFETFGFERMEEVHLFTKVFERVLSTTISHPEEATNEWELNYFGLQSWLKLVLADPKKSCEKEFSKIWESMRYLILFKHAWVRLSVSRLFGHFFAIIGDSNFGKLSLGIDGVVFSLDFVTQISNALQAQLRSPVLSEELGMQVAKNLIFLTRWFNSIRSSDDSPFLEIFRRMRKTLKKQTIEEYSINKKYLMQWFASVIHVFSGEELQPVLSEIIAALYRYTELQEAERKSQQELADLVTESLQVLQEKVGATVFARAYQEVRNAAIEVRRERREKRAIEQVVAPEVASRKKIRKNERKRENRKQKTNHHRMVNSIFKNR

Target ORF information:

RefSeq Version	NM_001022638.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative U3 snoRNP protein Utp20 (utp20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022638.2

ORF Insert Sequence:

ATGAAAGCTG CGGCGCAGAG CAATTCCAAA AACTATGTTT TTTTGCCTTT TTCCAAGAGA 
GTTGAAAATT TAAAGATTGA TGTAGCTCAT AAAATCCCTA GAGCTGCTGA TTTAGAGGAT 
GAAGACGTTG AGTCTTACTT TATTTCATGT CTCAGGAAAT GGGAGGACTT GAATCTTTCA 
ACCCACTATG TAAACTTTCT TCGTAGTGTC ACTCCATACT CACAGTCTTT ACCTCAAATA 
GTATATCATC AAAAAACAAT TTTTGATCTT ATTGTACAGT ATGCTCGTGA AGGAGATTCA 
CTGTCTTTAC AGCCAATCTT AGAATTATTG ACACAGTTTG CTCGTGATCT GGCACTGGAG 
TTTGCAAGTT ATATTGATCA AACCTTAGAA CTACTCTGCA TTCTCGTTCA GAATAACGAG 
TTGGAAGTTG TTGACTGGTC TTTTCATGCT GCTGCTTATC TTTTTAAATA TCTTCGAAAA 
ATTCTTGCAC CCCGTTTAAT TCATACTTAT GATATTCTAT CCCCTCTATT AGGTAAGGAA 
AAGCAGAAGA GTCATGTTAC TCGTTTTACC GCTGAGGCTT TATCTTTTTT GTGTAAAACA 
GTTTCTTACG AGAATGCAAT TGAGTTTACT GGACATGTGT TGCTCGATCT AAACGATCAC 
TATACTCCTC AATATCACGA GGGAGTCGTC ATTCTCTTTT CAAGGATAAT ACAGGGCGTT 
GATACGAGTA TTCACTTTAA AGGAAAAGCT TTTTTTGAAA TCCTATTACG ATCTGAGATT 
TACACCTTGC CAAGGTCTAA AAGTGTGATA ATTCCTGTCT TAATTTCTAC AATTCATCAT 
TGTACTTCAG ATTCCTTTAA CGAATTAGAA AAGTTAATAA TTTCTAAAAT AACCGGTGAG 
CCATTACTAT ATATAAATTT ATTTAAAGCT ACGTTGGTTA CCAGAAAGGG CTCTCGCGTA 
TCTGATTATC CGTCTTTCTT TAAAGCCTAT TTGCAGATAC CCAACTTAGT CAGCTGGGAA 
AATATAGAGT CAGATCTTTC AGTTCTGATA TTTGAAATAT CTGCTCTTCT TTTTGTATAT 
CCCCAAATTT CTGATTTAAT GCCTCACACG GTACGCATTA TTTCTTTTTT ACAACAAGGT 
CCATTACACT TGTTTTTCTC TTTTGTTGAT GTTGTGAAAC AGCTAAATAT GCAACGGTAT 
GATTCCTTCA TTAAACCATC TCTTCCCAAA TTTATTTCTT CACTAGTGAG TGATGAATCT 
CAGAAGTCGT TGGCAATTTT GGAAAGCGTT TTAGACAACT CTGTGCCCGT TCCAGTTGAT 
GTGGCTTCAG TCTCTCTTAA CCACTTTGTA TATTGTATTG AGAGACTTCA AATTGTTTCC 
CCGTCTTACG AAGATATCAT CTCCCTATGG TCTTCTTTGA TGATTATTAT TTCATCTTCA 
TTAGGATCTG ATGAAATCTA TAAGGCTTGC TTGCTTTTTT TAAGAAAGTT AGAAGATGTT 
TCATCTGAAT CAGTTTTATT AGGAGATATT TGTGGAATTG TATTGCATTT GCTTCAACGC 
AAAGTTTCTC GTTTATTTCT GGACAGCTCT TATTACAAGC CAATTCTAGA TTCTTTGCTT 
GGCGTTTTTG GTTTTTTAGC TGACAGTAAA GTCTTTTTAG AAAGTATTCG CCCCTTTTTC 
GGTACTTCAT ATGACTTTTG CAGCTCGTAT ACTTCATTAA TGGATCGTTT AATTAATAAT 
TTAAGTCGGG GGTTGACATC CCTAAGAGCG GCTTCTATTG ATTTGATTAT TGCATTGTGC 
AAATCTCTTC AGAAAAACGA GGTCCTACAA TCTCTATCTC TAGTAAAAAA GTTATCAGAG 
CTGCCTTTTG ATCCTTCAAC CAGTCGTGAT GCATCGGTCT TGCTACGAAA TTTGTCTGCC 
AAATCATCCG CTTTAGAGAA GGACACTAGA AGAGTTGTAT TACATGCCTT ACTAGGGCTA 
ACTATTACTC GATTCACCCC GCTTTGGCCG GATTTGTCAA GGACAGCAGC TTCCATCGTC 
ACGAAGGAAG TGGAAGATGA GTTTTTAGCT ATCATTTATT CTTGGCTTTC ACTTCCTTCT 
CCTCCCTCTG GTTTGTTAGG ACCAACTGAT ACTGCCGTGT TTGTGAAACC AGATTTGTCA 
CTAGAAAAGC TACCTACCTT AGAAATTAAC ACATTCAATT GTCCTGCGAT TTCTTACTTC 
GAAACATCTT TCGATGAGTG CTTTTCTAAG TTCGCTTCGT CTGACAACTA TATAATTGGA 
AACTTACTAA AAACAAATCA AGAAGATCTT TCAAATCTTC CGACTTTTCG GTCTCAGGCT 
TTACGAGTAC TCAATGAGCT TCCGGAAATA GCATCCCGTA ACATAAATGT TTTAGACAAT 
TATCTTTTTA GTTTACAACA TGGTTTCGAT CTTAATACGG AATGGGCTCG TCCTGATGTC 
TATTTGCTCT TAGGACTTTA CTCGAAATTC ACTGGCATCA AAGAGTTTGT CAATCGAGAT 
GCTCGAAAGG AATTTTTTCT TTGGGCCCTT ACTATTAATG ACCCAAAGGT TCAGAAATTG 
GTTCTAGACA TAATTCTTCT ATATTCAGAA GAAGCAATTA CTACTTACGA AGAAAATCTT 
CGAAACTTGC TGGATGATAA AAAGTGTAGA GATGAATTAA TTACATTTCT ATTTGTGGAT 
TACGCAGATA GCAAAATTCA AGATATTCAT CGTCCGTTAC TTATGCCTGT TGTAATATCC 
ATTCTCTACG GAAAAATGGT GTCTAAGGGA TATGGAGGAC AAAAGAATCA AGCTGCCAGA 
CGATCAACAA TTTTATCTGC ACTTGGGAAT ATGCAAGTAG AAGATTTACA GATTTTAGTT 
GATATTATGT TACGACCCTA TAATGGTCTT GAGGTCAAAT TGAACGCTAA TAATAATTTG 
GAGATCGACA CTGGAAACAT TCCTTCTTTG ACACTACGGC GACAAATTGG TTTTCTGACA 
ATGACCGAGG AACTTTTATT GCAGATTTCT TCTAAACTTT CTTCAGTTGC TCCCAAAATT 
CTGAATGCCG TTCTCTATAA TTTGGTTGTA TCCGATGATC AGATTTCCTC TCAAGAGGCT 
TTCGAGAATT TTGAGGTGAA AATGGCATAT ACGGTCAGAC AGCTATCACT GAAAGTTTTT 
CTACTTTTTT TGAAGTCCTG CTCTGATGTC GATTTCAAGC CTTATAATGT TTTTATATAT 
ACTGCTTTTG TGGTTCCACG CCTTGATCGG TTTGCTGATG AAAACACACA AAGTGTGTCA 
AATCTAATGA AAATTTTCCG TTGCTGGTTT GAGAATGAAG CCTATTTAGA TAGCGTCCTT 
GAGTTTTCTT CTCATATATT AACTGCTTTG CTTAACACTG CGTCTCACAC TGCCGTCAAA 
TTGCCGGTGT TGCTATACAT TCTTGATACT TTGAACTTGG TCATAACTCA TTTACAATCA 
GAAGAAAGTG AATCGCAGTT AAGAGAAAAG ATCTTGGCAA ATTTGGTTAT GCCGAACATC 
ACACTTATTT TTGCGAGTCT TTCGAATATT TTGAAGAATC CCCAATTTTG CAATAATAAT 
CGTGTAATGG ATGGATCCGT TCAGGCATTA TCTGCTGTAT CAGAATATAT GTCATTAGAT 
ATTGACTCTT CGCCTTTACT TAATCTGTTG GTTTCATTTT TGAGGAAACC CAACCGTCTG 
GTCCCTTCAA ATGTTAAGTC TAATATTTTA GTTTTGCTTT GTAAACTTCT TCCAACCAAT 
ACGAAATGGC TGCATGCTTC AATATCACAG ACAAATGATT TTGATACTAT TATGCATCTA 
TATACTTCTA TGGTTGACAT CAAGGCTCGT CAACATTTGA ACGATCTTTT GAAGATTTAT 
TCAACAATTG ATGATAACCT TATTTTCAGC TCAGTATTTG TTGAAGAAAT AAATTCTATT 
TCTAAAAAAC GGCTTGATGA ACCAGATTTT GAGCGTCGCT TATCTGCTTT TACCTCATTT 
AATGAAAAAC ACTTTTCTTT GATTTCAGAT TTAGCTTGGC TGCCTGTGCT CTATAACTTC 
TTCTTTTACG TTCAAGACGC TGAAGAGCTG GCTATTCGCG CTAGTGCGTC CCTGGGGATT 
AAACGGTTCA TTGAATCAAT AACTATGAAT GATGCATCAA ATCAGTTTAA GATTGATGTC 
TTTGTTAAGT TTATTTTTCC CTTCATCAAA AATCAAATGA AAAATAAAAA TGAACTCATT 
CGGCAAGAGT TTATTGGCTT GCTTAGTTAT TCTATTAAAT CGTTAACGAT GGTCGATGCA 
ATTTCGGATA TGCAACCTTT GCTTTACGAA GGTGATGAAG AAGCCAATTT TTTCAATAAC 
ATTTTGCATA TTCAGTTGCA CAGACGTAAA CGAGCAATGA AAAGGCTTGT TAATGTCTGT 
GCCATTGGTG TGATTAGAAG TGGTAATATT TCTCAAATAT TTCTACCCTT GCTGGAGAAC 
TTTTGTTTAG GTAATGATAC CGTGCAAACT TTGTTAGATG AATCAGTGAT CACAATTGGA 
GAAATCATAA AGTGGGCACA TTGGAATCAA TACCAAGCTA TTTTGAAGCG ATATGTTTCG 
CTGTTAAAAA ATAACGCTAT TGACCAAAAA GTAGTTGTTC GTTTGATAAC GGCCGTTGTG 
TCAGCATTAA GGCCATTGGA TGATGCAGTC GCTTCCTATA CAAACTCCGA AATGAACATA 
GAACAGTTTG ATGGCCAAAA GAAGAAATGC GTTTTAGCCA GTAGCCTACC TTCCGAAGAG 
AGATTTACAG AAGTCCTTAC TAATGATTTT TTTCCTACCC TGATGTTATA TCTACATATA 
AGAGACGAAT CAACGGTTAC CCTTCGTGTT GCAATTGCGC TTTCAATCGT GCAACTTGTT 
GCGCTTCTTC CCGAGGAAGA AATTGTTCTA CGGTTAACTC CTGTTTTGAT AGATACTTGC 
CATATTTTAC GCAGTCGCTC TTTAGAGTCG CGTGATGCAA CCCGGAAAGC TTTAGCAGCG 
ATATCAAAAT TTCTTGGCCC GAAGTACTTT TCCTTTATTA TCAGTCAATT ACAAACTTCT 
TTGAAAAGAG GGTATCAACT CCATGTTCTA GGTTACACAG TTCATTACCT CTTATTGGCG 
ATTGAGGACG TGTATCCGTA CGGCTCTATA GATTACTGTA TGGATTCTCT GGCTCAAATA 
TTTGTTGATG AGATATTTGG AGAAGTTGGT GTTGAAAAAG ATTCTGAAGA TTACAAATCA 
AATGTGAAGG AAATCAAAGG CAATAAGAGC TACGATTCTT ATGAGATTGT TGCTAGAATT 
TCTAGCTTCG ATAGTTTGAG TACATTACTT CGTCCGGTGA AGAATGTTTT GTTCGAAACA 
AATGTTCCTA AATCTCTACG AAAAGTAGAT GAATTGTGTC GGAGACTTTC TTTAGGAATT 
GTCGCGAACA AACAAAGTGC TTCGCAGAGT TCTTTGATTT TCTGTTATAA TGTTTACGAA 
TTTGTTGTCA AGGAAAAGGA AACTGTTGCG GCATTGAAAC AGCAAGAAAA CGATGGTTAT 
CGGTCAGCTC CTAATTTCTT TTTAGAAAAC TCAAAGAAGT TAATAAGGTT TACTTTCGAT 
GTTTTACGTG GTGTATCTAA CAAACATAAA GAATTGTTGA CAGCCAGAAA CATGGCGGCT 
TTCGTGCCTC TAATCGGTGA ATCGCTTTTA AGTAGCTCTG AAGAAGTTCA AATTTCTGCA 
TTACGCTTTC TCGTTTTGCT TCTCCCTTTA AAAATTGATC AGGTTTTTTC GGGTAGTTCA 
GTCTTCACGT CTCAGGCTGT GAAATATATA CAGAATTCGC CTTCTACAAA CACAGAATTA 
TGTCAAGCAA GTTTTAAGTT TTTAGCATCT ATTTTACCTT ATGAAAATGT AAAGATTAAG 
GAATCGACTA TAAACTATTT ACTGGAAAGG GTTGGAACTG ATATTCAAGA ACCTGATCGT 
CAAGGTGTTA TGTTTTCGTT GGTTAGAGCG GTTATTGCTC GCAAAATCAT GACTCCTGAA 
CTGTACAAAA TAATTGATTT AATACGAGAT ATGATGGTGA CAAATCATAC TAAATCCACT 
CGTCAAACTT GTCGTCATTT GTATTATTCA TTTCTTCTTG ATTATCCCCA GGGTAAAACT 
CGACTATCCA AGCAAATATC ATTTATTCTT AAGAACTTGG AATATGAATT CGCACCCGGA 
CGCGAATCTG TAATGGAGCT CTTGCATCTT ATCTTGAATA ATTTTAGTGA TGCTTTATTG 
AAAGAGTATC ATCAAGGAAT ATTTATTGCT CTAGTTATGG TGCTAGCTAA TGACTCAGAA 
CCTCATTGTA GAGAGATGTC TGCAGAATTA ATAAAGCTCG TATATCAACG TGCTGACAAT 
GAGAATTTTA ATTTGATTCG ACAGTTGTTG AGTCATTGGA CTTCAGTTGA GAAAGCCGGG 
AAAAATTTGG TCCGTGTAAG TATGCAGCTG TTTGGTTTAT TATTTGAAAC TTTTGGCTTC 
GAGAGGATGG AAGAAGTGCA CCTTTTTACG AAAGTTTTTG AAAGGGTTCT TTCAACAACT 
ATTAGCCATC CAGAAGAAGC AACAAATGAA TGGGAGTTAA ACTATTTTGG TCTCCAATCT 
TGGTTGAAGC TGGTCTTGGC GGATCCCAAA AAGTCATGTG AAAAGGAGTT TTCAAAGATT 
TGGGAATCAA TGCGTTATCT TATACTTTTT AAGCATGCAT GGGTTCGACT TTCTGTCTCC 
CGTCTCTTTG GTCACTTTTT TGCAATAATC GGAGACTCAA ATTTTGGAAA GCTTTCATTA 
GGAATTGACG GAGTTGTTTT CAGTTTAGAC TTTGTAACGC AAATATCAAA TGCTTTACAA 
GCACAATTAC GTAGTCCGGT TCTTTCCGAA GAGTTGGGCA TGCAGGTTGC TAAAAACTTA 
ATATTCCTTA CTCGCTGGTT TAATAGCATT CGCAGTTCTG ATGATTCTCC TTTTTTGGAA 
ATATTTAGAA GAATGCGCAA AACCCTCAAG AAGCAAACTA TTGAAGAATA TAGCATCAAC 
AAGAAATACT TGATGCAATG GTTTGCTAGC GTTATCCATG TTTTCTCTGG TGAAGAATTG 
CAACCGGTCC TTTCTGAAAT TATTGCGGCT TTATATCGCT ATACAGAACT ACAGGAGGCT 
GAAAGGAAAT CGCAACAGGA ACTTGCAGAT TTAGTGACTG AGTCATTACA AGTGCTTCAG 
GAAAAAGTTG GCGCGACTGT GTTTGCTAGG GCTTATCAAG AAGTACGAAA TGCTGCTATC 
GAAGTTCGCA GAGAAAGACG TGAGAAACGA GCTATTGAGC AAGTTGTAGC TCCTGAAGTT 
GCTTCTCGCA AGAAAATTCG AAAGAATGAA AGAAAACGAG AAAATAGGAA ACAAAAAACA 
AACCATCATC GCATGGTAAA TTCAATTTTT AAAAATAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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utp20 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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