bgs1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	bgs1
Entrez Gene ID	2540737
Full Name	1,3-beta-glucan synthase catalytic subunit Bgs1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021879.2	NP_595971.1	1,3-beta-glucan synthase catalytic subunit Bgs1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sbg1p and Bgs1p physically interact and are dependent on each other to localize to the division site. Loss of Sbg1p results in an unstable actomyosin ring that unravels and slides, leading to an inability to deposit a single contiguous division septum and an important reduction of the beta-1,3-glucan proportion in the cell wal
Title: A New Membrane Protein Sbg1 Links the Contractile Ring Apparatus and Septum Synthesis Machinery in Fission Yeast.

Sbg1 and Bgs1 physically interact and are interdependent for localization to the plasma membrane
Title: Sbg1 Is a Novel Regulator for the Localization of the ?-Glucan Synthase Bgs1 in Fission Yeast.

cytokinesis machinery integrity under stress conditions requires the glucan synthase Bgs1p and its regulator Cfh3p
Title: The integrity of the cytokinesis machinery under stress conditions requires the glucan synthase Bgs1p and its regulator Cfh3p.

Findings reveal a role for Sip1 in the regulation of Golgi/endosome trafficking in coordination with the AP-1 complex, and identified Bgs1, required for cell wall synthesis, as the new cargo of AP-1-dependent trafficking.
Title: Sip1, a conserved AP-1 accessory protein, is important for Golgi/endosome trafficking in fission yeast.

fission yeast SEL1 domain protein Cfh3p is a novel regulator of the glucan synthase Bgs1p whose function is more relevant under stress conditions
Title: The fission yeast SEL1 domain protein Cfh3p: a novel regulator of the glucan synthase Bgs1p whose function is more relevant under stress conditions.

The following bgs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bgs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100391	NM_001021879.2 Latest version!	Schizosaccharomyces pombe 1,3-beta-glucan synthase catalytic subunit Bgs1 (bgs1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bgs1 ( NM_001021879.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595971.1 Schizosaccharomyces pombe 1,3-beta-glucan synthase catalytic subunit Bgs1 (bgs1), mRNA.

Price & Availability↑

CloneID	OSc100391
Clone ID Related Accession (Same CDS sequence)	NM_001021879.2
Accession Version	NM_001021879.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	1,3-beta-glucan synthase catalytic subunit Bgs1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021879.1.

ATGGATCAGT ATTGGCGTGA ACAAGAGGGT CGTGGACTCT TTGAAGATGA TGCCAATAGT 
TATGTCTCTG ACGATGATAC TATGAGCTCT CTGACCCGTA TGATTTACGA TAAAAACTCT 
TCTCGTGATG CATTACATTC TGATTATGAT AGCTCTTCAT TTAATGTCGA TTCCAGTTCA 
GTTGCTTATC CGGCTTGGAA TCAAGCTGGT GAAGAGGCTC CTGTTACTAT GGAAGGTGTT 
CAAGAGATCT TGTTGGACCT TACAAATAAA CTTGGTTTTC AAAAAGATAA CATGCGTAAC 
ATTTTTGATT ATGTTATGGT TCTTTTAGAT TCTCGGGCAT CTCGTATGTC TCCTTCCTCG 
GCACTGCTTA CTATCCATGC CGATGTCATT GGTGGTGAAC ATGCAAATTT TTCAAAGTGG 
TACTTTGCTT CTCATTTTAA TGACGGTCAT GCCATTGGAT TTCACGATAT GTCTTCCCCA 
ATTGTCGAAA CAATGACTTT GAAAGAAGCT GAACAGGCAT GGAGAGATCA AATGGCAGCA 
TTTTCACCCC ATCGCATGAT GGTGCAGGTG TGCCTTTATT TTCTTTGCTG GGGTGAAGCT 
AACAATGTTC GTTTTGTTCC CGAGTGTCTT TGTTTTATAT TTGAATGTGC TTATGATTAT 
TATATCTCTT CGGAAGCTAA AGATGTCGAT GCTGCTTTGC CCAAGGAATT TTATTTAGAC 
TCGGTTATCA CCCCTATTTA TCGATTTATT CATGCACAGC TGTTTGAAAT TTTGGACGGC 
AAATACGTCA GACGAGAAAG AGATCATTCT CAAATCATTG GCTATGATGA TATTAATCAA 
TTGTTTTGGT CCTACAAGGG CCTTCAGGAA ATCATGTGTG CTGATAAAAC TCCGTTGTTA 
GATTTACCAC CGTTTATGCG ATATCGACAT CTTTCCGATG TTGAATGGAA ATCATGTTTT 
TATAAGTCCT ATTACGAGTA TCGTTCTTGG TTCCATAACG TTACCAATTT CTCACGTATT 
TGGGTAATGC ATATATCTGC TTACTGGTAT TATTCGGCTT ATAATTCCCC AAATTTGTAC 
ACCAAAAATT ATCACATCCG CTTGAATAAT AAACCTCCTG CTTCTTGTCG TTGGACAGCT 
TGTGGTTTAG CTGGTGCTAT AGCTTCTTTT ATCACCTTGG CAGCTGTGGT TTTCGAATAT 
ATACATGTTC CTCGTCGTTA TCATTCTGCC AGAAGGTTGT GGCCGAGTAT GCTGTTATTG 
ATAAGTACGC TACTTCTAAA CATCGCACCT GTTGTTTTCA TATTTGCTTC GTCTACGAAA 
GAGCAGCATT ATGCTTCTCG ATTGGTTGTA GGAATTGTGC ATTTCTTTTT CTCGTTGGTC 
TGTGTAGTTT ACTATTCGAT TACGCCGTTA CGAAATTTGG TTGGTTTCAC CACAAAAAGG 
TCTGGAAAAA ACCTTGCTAA CAGATTTTTC ACCGCCAATT TCACTCCGAC CTCAAAAACA 
GGTGCTTTCG TTTCTTGGTG TCTTTGGATT ACAGTTTTGG TGGCCAAATT CTTGGAATCT 
TATTTCTTTT TAACTTTGAA TTTGGCAGAT TCTATCAGAT TTTTGGGTGC TATGCGACCT 
TATGACTGCC GTGATTACAT TTTAGGAGCT GGACTATGCA AGGCTCAGCC TAAAATTCTC 
TTGAGTTTAC TGTATTTAAC TGATCTTTCT CTGTTTTTCC TTGATACTTA TCTATGGTAC 
ATTCTTATCA GCACTATCTA TTCTTTGGCT TATGCTTTTT GCCTGGGTAT CAGTGTGTGG 
ACTCCTTGGA GAGAATTGTT TTACCGCGTT CCCAGAAGAA TATATACAAA ACTGTTGTAT 
ACCGATGACA TGGAGATTGT CTTCAAGCCA AAAGTACTTA TTTCTCAAGT TTGGAATGCC 
ATTATTATCT CGATGTACAG GGAGCATTTG ATTTCCCGTA CCCAAATTCA GGAGTTGTTG 
TACCATCAAG TACCTTCTGA AAAGGCAGGC TATCATACTC TTAGAGCTCC CAACTTTTTC 
TACTCCCAAC AGGTTAAGCA TTATAAGCAA GATTTGTTTC CGGCTAATTC AGAAGCTGCT 
CGAAGAATCT CCTTTTTCGC ACAATCCTTG GCTGAATCAA TACCCAAGAC TAGTTCCATT 
GATGCCATGC CTACCTTCAC TGTTTTGGTT CCTCACTATT CTGAGAAAAT TTTGTTATCT 
TTAAGAGAGA TTATTCGCGA GGAAGATCAA CTTTCTCGCG TAACTCTATT AGAGTACTTG 
AAGCAGCTAT ATCCTGTGGA GTGGAGAAAT TTTGTTGATG ATACAAAATT ACTTGCTGAT 
GAAAATGATT CTGTTATTGG CAGTATCGAC AATGAAAAAA ATGGAGTAAA TAAAGCATAT 
GACCTTCCTT TCTATTGTGT TGGATTTAAA TCTGCGACTC CGGAGTATAC TTTGAGAACA 
AGAATTTGGG CCTCTCTGAG AACTCAAACT TTGTATCGCA CTATCAACGG TTTCAGTAAC 
TATAGTCGTG CCATCAAACT GTTATACAGA ACGGAAACAC CAGAATTGGT CGAATGGACA 
AATGGTGACC CTGTGCGATT AGATGAAGAA TTGGATTTAA TGGCAAACAG GAAGTTCCGA 
TTCTGTGTCT CCATGCAGCG GTATGCTAAG TTTACCAAAG AAGAAGCCGA AAATGCAGAA 
TTTTTATTAA GAGCGTATCC AGACTTGCAA ATTGCATATA TGGATGAAGA TCCTCAATCT 
CGACATAATG ACGAGCGCCA TCTTTATTCT GTACTGATTG ACGGTCATTG CCCCATTATG 
GAAAATGGAA AGCGTCGACC CAAATATCGT ATTCGTCTAT CTGGTAACCC AATTTTGGGT 
GACGGTAAAT CTGATAATCA AAATATGTCT ATTCCTTACA TTAGAGGTGA ATATGTACAA 
ATGATCGATG CCAACCAGGA TAATTATTTG GAAGAATGTC TAAAGATTCG TAGCATTCTT 
GCTGAATTTG AGCAACTTAC TCCTCCCTTA CATAGCCCTT ACTCGGTAAA TGCCAAAGCT 
GCTGACAATC ATCCCGTAGC TATTCTCGGT GCTAGAGAAT ACATTTTTTC AGAAAATACA 
GGTATGCTTG GAGATGTAGC TGCTGGAAAG GAACAAACAT TTGGTACTCT TTTCGCTCGT 
ATTTTATCGT TGATTGGTGG AAAATTGCAT TACGGACATC CTGATTTTAT TAATGTTTTG 
TTTATGATTA CTCGTGGCGG CGTTTCAAAA GCACAAAAAG GTCTGCATGT TAATGAAGAT 
ATTTATGCAG GTATGATTGC TCTTCAACGT GGAGGACGTA TTAAGCATTG TGATTACTAT 
CAATGTGGTA AGGGTCGTGA CTTGGGTTTT GGCTCAATTC TTAACTTTAC TACGAAAATT 
GGAACGGGTA TGGCGGAACA AATGCTTTCA AGAGAATATT TCAACCTTGG CACACAATTA 
CCGTTTGATA GGTTTTTGTC ATTTTTCTAT GCCCACGCTG GATTTCACGT CAATAACATG 
GTCATCATGT TTTCTTTACA GTTATTGATG CTTGTTATTA TTAATTTGGG AGCAATGTAC 
ACTGTTGTTC CGGTTTGTAG ATACCGCCAA TTTGACTCCT TGACAGCTTC TTTGTACCCT 
GAAGGTTGTT ATCAATTAAA ACCAGTTCTC GAGTGGTTGA AGCGTTGTAT TTTGTCTATT 
TTCATTGTCT TTGGTATTGC TTTTGTTCCA CTTGCTGTTT GTGAATTAGG GGAACGCGGT 
GCCATCCGAA TGGTTATTCG TTTAGCGAAA CAAATATTCT CTCTATCTCC TATTTTTGAA 
ATTTTCACAT GCCAAATATA TGCGCAGAGT TTAATTGCAA ATCTTACGTT TGGTGGTGCA 
CGCTATATTG GTACTAGCAG AGGTTTTGCT ACTGTTCGTG TGCCGTTCTC ACTATTGTAT 
TCTCGATTCT CTGGACCATC ACTTTATTTT GGGTCACGAC TTATGTACAT GTTATTGTTT 
GGTTCAATTA CTGCTTGGTT GCCGCATTAT ATTTACTTTT GGATTACGCT GACTGCTCTT 
TGCATTAGTC CATTCCTTTA CAATCCCCAT CAATTCGCCT GGACCGATTT CTTTGTTGAT 
TATCGTGAGT TTATGCGCTG GCTATTCCGC GAAAATTCAC GCAATCAAGC AAATTCATGG 
ATTGGCAACT GTCAACTTTG CCGTACCAGA GTAACTGGGT ATAAACGTAA AATATATGGT 
AAGAAGGCAG ATAAAATTGC TATGGATTCC CCGCGTGCGA GAATTACTAC AATGTTTTAT 
GGTGAAATAT TAGGGCCTCT AGGAACACTA TTTTTCACGT GTATTCCATT TCTCTTTATC 
AATTCCCAAC CTGGTAACGA TGATGAGACC CAATCTACTA ATGCTTTTAT TCGCTTGATT 
ATTATGTCAG TTGCTCCTTT GGTTTTAAGT GCAATTATTG CTTTTTTCTT TTTCTGTTTA 
GGAATCATGT TACGTCCTAT TCTCGGCGAT AGATCTAAAA CGTATGGTGT ATATTTGGCT 
GGAGTGGCCC ATTTCCTTTT TGTGTGTGTT GATGTTGTTG TCTTTGAAGT ATTGGGCTAT 
TTGGAAGGTT GGTCATTTTC AAAAACTTTA CTTGGATTTG TAGCAATCAT TTCAATTCAC 
AGGTTTGCTC ATAAATTTTT TATCATATGT TTCCTGTCTC GAGAATTTCG TCATGATGGT 
GCCAATTTAG CTTGGTGGTC TGGACGTTGG AATGGCCAAG GTTTTGGATA TATGGTTTTA 
ACGCAACCAT GGAGAGAATT TGTGTGTAAA ACTACAGAGT TGAATATGTT TGCTGGTGAT 
TTCCTTTTAA GTCATCTTTT GTTGTTTTTG CAAGCGCCTG TTATTTTGAT TCCTTATATC 
GACAAACTTC ACTCGATCAT ATTATTCTGG CTTGTGCCCA GCAGACAAAT TCGACCCCCA 
ATTTATACCA TAAGACAAAA CAAACTTCGT CGTCAAATAG TTCTTCGCTA TGCCACTTTG 
TACTTTTCAT TGTTTATTGC CTTTTTTGTT CTTTTAATTT TGCCTTTTGT GTTTGGTAAA 
TCAGCTGCTG GAACTTCAAT GGATAAATTT AATCTGATTC AGCCAGCGAC AAAGATTGTT 
TATTCTTCAA CAAAGAATTC TTCTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595971.1
CDS	1163..6352
Translation	MDQYWREQEGRGLFEDDANSYVSDDDTMSSLTRMIYDKNSSRDALHSDYDSSSFNVDSSSVAYPAWNQAGEEAPVTMEGVQEILLDLTNKLGFQKDNMRNIFDYVMVLLDSRASRMSPSSALLTIHADVIGGEHANFSKWYFASHFNDGHAIGFHDMSSPIVETMTLKEAEQAWRDQMAAFSPHRMMVQVCLYFLCWGEANNVRFVPECLCFIFECAYDYYISSEAKDVDAALPKEFYLDSVITPIYRFIHAQLFEILDGKYVRRERDHSQIIGYDDINQLFWSYKGLQEIMCADKTPLLDLPPFMRYRHLSDVEWKSCFYKSYYEYRSWFHNVTNFSRIWVMHISAYWYYSAYNSPNLYTKNYHIRLNNKPPASCRWTACGLAGAIASFITLAAVVFEYIHVPRRYHSARRLWPSMLLLISTLLLNIAPVVFIFASSTKEQHYASRLVVGIVHFFFSLVCVVYYSITPLRNLVGFTTKRSGKNLANRFFTANFTPTSKTGAFVSWCLWITVLVAKFLESYFFLTLNLADSIRFLGAMRPYDCRDYILGAGLCKAQPKILLSLLYLTDLSLFFLDTYLWYILISTIYSLAYAFCLGISVWTPWRELFYRVPRRIYTKLLYTDDMEIVFKPKVLISQVWNAIIISMYREHLISRTQIQELLYHQVPSEKAGYHTLRAPNFFYSQQVKHYKQDLFPANSEAARRISFFAQSLAESIPKTSSIDAMPTFTVLVPHYSEKILLSLREIIREEDQLSRVTLLEYLKQLYPVEWRNFVDDTKLLADENDSVIGSIDNEKNGVNKAYDLPFYCVGFKSATPEYTLRTRIWASLRTQTLYRTINGFSNYSRAIKLLYRTETPELVEWTNGDPVRLDEELDLMANRKFRFCVSMQRYAKFTKEEAENAEFLLRAYPDLQIAYMDEDPQSRHNDERHLYSVLIDGHCPIMENGKRRPKYRIRLSGNPILGDGKSDNQNMSIPYIRGEYVQMIDANQDNYLEECLKIRSILAEFEQLTPPLHSPYSVNAKAADNHPVAILGAREYIFSENTGMLGDVAAGKEQTFGTLFARILSLIGGKLHYGHPDFINVLFMITRGGVSKAQKGLHVNEDIYAGMIALQRGGRIKHCDYYQCGKGRDLGFGSILNFTTKIGTGMAEQMLSREYFNLGTQLPFDRFLSFFYAHAGFHVNNMVIMFSLQLLMLVIINLGAMYTVVPVCRYRQFDSLTASLYPEGCYQLKPVLEWLKRCILSIFIVFGIAFVPLAVCELGERGAIRMVIRLAKQIFSLSPIFEIFTCQIYAQSLIANLTFGGARYIGTSRGFATVRVPFSLLYSRFSGPSLYFGSRLMYMLLFGSITAWLPHYIYFWITLTALCISPFLYNPHQFAWTDFFVDYREFMRWLFRENSRNQANSWIGNCQLCRTRVTGYKRKIYGKKADKIAMDSPRARITTMFYGEILGPLGTLFFTCIPFLFINSQPGNDDETQSTNAFIRLIIMSVAPLVLSAIIAFFFFCLGIMLRPILGDRSKTYGVYLAGVAHFLFVCVDVVVFEVLGYLEGWSFSKTLLGFVAIISIHRFAHKFFIICFLSREFRHDGANLAWWSGRWNGQGFGYMVLTQPWREFVCKTTELNMFAGDFLLSHLLLFLQAPVILIPYIDKLHSIILFWLVPSRQIRPPIYTIRQNKLRRQIVLRYATLYFSLFIAFFVLLILPFVFGKSAAGTSMDKFNLIQPATKIVYSSTKNSSV

Target ORF information:

RefSeq Version	NM_001021879.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe 1,3-beta-glucan synthase catalytic subunit Bgs1 (bgs1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021879.2

ORF Insert Sequence:

ATGGATCAGT ATTGGCGTGA ACAAGAGGGT CGTGGACTCT TTGAAGATGA TGCCAATAGT 
TATGTCTCTG ACGATGATAC TATGAGCTCT CTGACCCGTA TGATTTACGA TAAAAACTCT 
TCTCGTGATG CATTACATTC TGATTATGAT AGCTCTTCAT TTAATGTCGA TTCCAGTTCA 
GTTGCTTATC CGGCTTGGAA TCAAGCTGGT GAAGAGGCTC CTGTTACTAT GGAAGGTGTT 
CAAGAGATCT TGTTGGACCT TACAAATAAA CTTGGTTTTC AAAAAGATAA CATGCGTAAC 
ATTTTTGATT ATGTTATGGT TCTTTTAGAT TCTCGGGCAT CTCGTATGTC TCCTTCCTCG 
GCACTGCTTA CTATCCATGC CGATGTCATT GGTGGTGAAC ATGCAAATTT TTCAAAGTGG 
TACTTTGCTT CTCATTTTAA TGACGGTCAT GCCATTGGAT TTCACGATAT GTCTTCCCCA 
ATTGTCGAAA CAATGACTTT GAAAGAAGCT GAACAGGCAT GGAGAGATCA AATGGCAGCA 
TTTTCACCCC ATCGCATGAT GGTGCAGGTG TGCCTTTATT TTCTTTGCTG GGGTGAAGCT 
AACAATGTTC GTTTTGTTCC CGAGTGTCTT TGTTTTATAT TTGAATGTGC TTATGATTAT 
TATATCTCTT CGGAAGCTAA AGATGTCGAT GCTGCTTTGC CCAAGGAATT TTATTTAGAC 
TCGGTTATCA CCCCTATTTA TCGATTTATT CATGCACAGC TGTTTGAAAT TTTGGACGGC 
AAATACGTCA GACGAGAAAG AGATCATTCT CAAATCATTG GCTATGATGA TATTAATCAA 
TTGTTTTGGT CCTACAAGGG CCTTCAGGAA ATCATGTGTG CTGATAAAAC TCCGTTGTTA 
GATTTACCAC CGTTTATGCG ATATCGACAT CTTTCCGATG TTGAATGGAA ATCATGTTTT 
TATAAGTCCT ATTACGAGTA TCGTTCTTGG TTCCATAACG TTACCAATTT CTCACGTATT 
TGGGTAATGC ATATATCTGC TTACTGGTAT TATTCGGCTT ATAATTCCCC AAATTTGTAC 
ACCAAAAATT ATCACATCCG CTTGAATAAT AAACCTCCTG CTTCTTGTCG TTGGACAGCT 
TGTGGTTTAG CTGGTGCTAT AGCTTCTTTT ATCACCTTGG CAGCTGTGGT TTTCGAATAT 
ATACATGTTC CTCGTCGTTA TCATTCTGCC AGAAGGTTGT GGCCGAGTAT GCTGTTATTG 
ATAAGTACGC TACTTCTAAA CATCGCACCT GTTGTTTTCA TATTTGCTTC GTCTACGAAA 
GAGCAGCATT ATGCTTCTCG ATTGGTTGTA GGAATTGTGC ATTTCTTTTT CTCGTTGGTC 
TGTGTAGTTT ACTATTCGAT TACGCCGTTA CGAAATTTGG TTGGTTTCAC CACAAAAAGG 
TCTGGAAAAA ACCTTGCTAA CAGATTTTTC ACCGCCAATT TCACTCCGAC CTCAAAAACA 
GGTGCTTTCG TTTCTTGGTG TCTTTGGATT ACAGTTTTGG TGGCCAAATT CTTGGAATCT 
TATTTCTTTT TAACTTTGAA TTTGGCAGAT TCTATCAGAT TTTTGGGTGC TATGCGACCT 
TATGACTGCC GTGATTACAT TTTAGGAGCT GGACTATGCA AGGCTCAGCC TAAAATTCTC 
TTGAGTTTAC TGTATTTAAC TGATCTTTCT CTGTTTTTCC TTGATACTTA TCTATGGTAC 
ATTCTTATCA GCACTATCTA TTCTTTGGCT TATGCTTTTT GCCTGGGTAT CAGTGTGTGG 
ACTCCTTGGA GAGAATTGTT TTACCGCGTT CCCAGAAGAA TATATACAAA ACTGTTGTAT 
ACCGATGACA TGGAGATTGT CTTCAAGCCA AAAGTACTTA TTTCTCAAGT TTGGAATGCC 
ATTATTATCT CGATGTACAG GGAGCATTTG ATTTCCCGTA CCCAAATTCA GGAGTTGTTG 
TACCATCAAG TACCTTCTGA AAAGGCAGGC TATCATACTC TTAGAGCTCC CAACTTTTTC 
TACTCCCAAC AGGTTAAGCA TTATAAGCAA GATTTGTTTC CGGCTAATTC AGAAGCTGCT 
CGAAGAATCT CCTTTTTCGC ACAATCCTTG GCTGAATCAA TACCCAAGAC TAGTTCCATT 
GATGCCATGC CTACCTTCAC TGTTTTGGTT CCTCACTATT CTGAGAAAAT TTTGTTATCT 
TTAAGAGAGA TTATTCGCGA GGAAGATCAA CTTTCTCGCG TAACTCTATT AGAGTACTTG 
AAGCAGCTAT ATCCTGTGGA GTGGAGAAAT TTTGTTGATG ATACAAAATT ACTTGCTGAT 
GAAAATGATT CTGTTATTGG CAGTATCGAC AATGAAAAAA ATGGAGTAAA TAAAGCATAT 
GACCTTCCTT TCTATTGTGT TGGATTTAAA TCTGCGACTC CGGAGTATAC TTTGAGAACA 
AGAATTTGGG CCTCTCTGAG AACTCAAACT TTGTATCGCA CTATCAACGG TTTCAGTAAC 
TATAGTCGTG CCATCAAACT GTTATACAGA ACGGAAACAC CAGAATTGGT CGAATGGACA 
AATGGTGACC CTGTGCGATT AGATGAAGAA TTGGATTTAA TGGCAAACAG GAAGTTCCGA 
TTCTGTGTCT CCATGCAGCG GTATGCTAAG TTTACCAAAG AAGAAGCCGA AAATGCAGAA 
TTTTTATTAA GAGCGTATCC AGACTTGCAA ATTGCATATA TGGATGAAGA TCCTCAATCT 
CGACATAATG ACGAGCGCCA TCTTTATTCT GTACTGATTG ACGGTCATTG CCCCATTATG 
GAAAATGGAA AGCGTCGACC CAAATATCGT ATTCGTCTAT CTGGTAACCC AATTTTGGGT 
GACGGTAAAT CTGATAATCA AAATATGTCT ATTCCTTACA TTAGAGGTGA ATATGTACAA 
ATGATCGATG CCAACCAGGA TAATTATTTG GAAGAATGTC TAAAGATTCG TAGCATTCTT 
GCTGAATTTG AGCAACTTAC TCCTCCCTTA CATAGCCCTT ACTCGGTAAA TGCCAAAGCT 
GCTGACAATC ATCCCGTAGC TATTCTCGGT GCTAGAGAAT ACATTTTTTC AGAAAATACA 
GGTATGCTTG GAGATGTAGC TGCTGGAAAG GAACAAACAT TTGGTACTCT TTTCGCTCGT 
ATTTTATCGT TGATTGGTGG AAAATTGCAT TACGGACATC CTGATTTTAT TAATGTTTTG 
TTTATGATTA CTCGTGGCGG CGTTTCAAAA GCACAAAAAG GTCTGCATGT TAATGAAGAT 
ATTTATGCAG GTATGATTGC TCTTCAACGT GGAGGACGTA TTAAGCATTG TGATTACTAT 
CAATGTGGTA AGGGTCGTGA CTTGGGTTTT GGCTCAATTC TTAACTTTAC TACGAAAATT 
GGAACGGGTA TGGCGGAACA AATGCTTTCA AGAGAATATT TCAACCTTGG CACACAATTA 
CCGTTTGATA GGTTTTTGTC ATTTTTCTAT GCCCACGCTG GATTTCACGT CAATAACATG 
GTCATCATGT TTTCTTTACA GTTATTGATG CTTGTTATTA TTAATTTGGG AGCAATGTAC 
ACTGTTGTTC CGGTTTGTAG ATACCGCCAA TTTGACTCCT TGACAGCTTC TTTGTACCCT 
GAAGGTTGTT ATCAATTAAA ACCAGTTCTC GAGTGGTTGA AGCGTTGTAT TTTGTCTATT 
TTCATTGTCT TTGGTATTGC TTTTGTTCCA CTTGCTGTTT GTGAATTAGG GGAACGCGGT 
GCCATCCGAA TGGTTATTCG TTTAGCGAAA CAAATATTCT CTCTATCTCC TATTTTTGAA 
ATTTTCACAT GCCAAATATA TGCGCAGAGT TTAATTGCAA ATCTTACGTT TGGTGGTGCA 
CGCTATATTG GTACTAGCAG AGGTTTTGCT ACTGTTCGTG TGCCGTTCTC ACTATTGTAT 
TCTCGATTCT CTGGACCATC ACTTTATTTT GGGTCACGAC TTATGTACAT GTTATTGTTT 
GGTTCAATTA CTGCTTGGTT GCCGCATTAT ATTTACTTTT GGATTACGCT GACTGCTCTT 
TGCATTAGTC CATTCCTTTA CAATCCCCAT CAATTCGCCT GGACCGATTT CTTTGTTGAT 
TATCGTGAGT TTATGCGCTG GCTATTCCGC GAAAATTCAC GCAATCAAGC AAATTCATGG 
ATTGGCAACT GTCAACTTTG CCGTACCAGA GTAACTGGGT ATAAACGTAA AATATATGGT 
AAGAAGGCAG ATAAAATTGC TATGGATTCC CCGCGTGCGA GAATTACTAC AATGTTTTAT 
GGTGAAATAT TAGGGCCTCT AGGAACACTA TTTTTCACGT GTATTCCATT TCTCTTTATC 
AATTCCCAAC CTGGTAACGA TGATGAGACC CAATCTACTA ATGCTTTTAT TCGCTTGATT 
ATTATGTCAG TTGCTCCTTT GGTTTTAAGT GCAATTATTG CTTTTTTCTT TTTCTGTTTA 
GGAATCATGT TACGTCCTAT TCTCGGCGAT AGATCTAAAA CGTATGGTGT ATATTTGGCT 
GGAGTGGCCC ATTTCCTTTT TGTGTGTGTT GATGTTGTTG TCTTTGAAGT ATTGGGCTAT 
TTGGAAGGTT GGTCATTTTC AAAAACTTTA CTTGGATTTG TAGCAATCAT TTCAATTCAC 
AGGTTTGCTC ATAAATTTTT TATCATATGT TTCCTGTCTC GAGAATTTCG TCATGATGGT 
GCCAATTTAG CTTGGTGGTC TGGACGTTGG AATGGCCAAG GTTTTGGATA TATGGTTTTA 
ACGCAACCAT GGAGAGAATT TGTGTGTAAA ACTACAGAGT TGAATATGTT TGCTGGTGAT 
TTCCTTTTAA GTCATCTTTT GTTGTTTTTG CAAGCGCCTG TTATTTTGAT TCCTTATATC 
GACAAACTTC ACTCGATCAT ATTATTCTGG CTTGTGCCCA GCAGACAAAT TCGACCCCCA 
ATTTATACCA TAAGACAAAA CAAACTTCGT CGTCAAATAG TTCTTCGCTA TGCCACTTTG 
TACTTTTCAT TGTTTATTGC CTTTTTTGTT CTTTTAATTT TGCCTTTTGT GTTTGGTAAA 
TCAGCTGCTG GAACTTCAAT GGATAAATTT AATCTGATTC AGCCAGCGAC AAAGATTGTT 
TATTCTTCAA CAAAGAATTC TTCTGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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bgs1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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