tor2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	tor2
Entrez Gene ID	2540720
Full Name	phosphatidylinositol kinase Tor2
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021266.3	NP_595359.2	phosphatidylinositol kinase Tor2

Pathways from BioSystems

Homologies

Homo sapiens (human)	MTOR	NP_004949.1
Canis lupus familiaris (dog)	MTOR	XP_005618060.1
Gallus gallus (chicken)	MTOR	XP_417614.3
Danio rerio (zebrafish)	mtor	NP_001070679.2
Schizosaccharomyces pombe (fission yeast)	tor2	NP_595359.2
Kluyveromyces lactis	KLLA0B13948g	XP_452152.1
Neurospora crassa	NCU05608	XP_960570.1
Pan troglodytes (chimpanzee)	MTOR	XP_003949341.1
Bos taurus (cattle)	MTOR	XP_005217047.1
Drosophila melanogaster (fruit fly)	Tor	NP_001260427.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007873	XP_317619.4
Arabidopsis thaliana (thale cress)	TOR	NP_175425.2
Xenopus tropicalis (tropical clawed frog)	mtor	XP_004916088.1
Rattus norvegicus (Norway rat)	Mtor	NP_063971.1
Oryza sativa (rice)	Os05g0235300	NP_001054988.2
Mus musculus (house mouse)	Mtor	NP_064393.2
Saccharomyces cerevisiae (baker's yeast)	TOR2	NP_012719.2
Eremothecium gossypii	AGOS_AFR420W	NP_985967.2
Magnaporthe oryzae (rice blast fungus)	MGG_15156	XP_003710485.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Since Gaf1 was also critical for transcription of per1+ and put4+, Tor-Gaf1 signaling may coordinate transcription of multiple amino acid permeases according to nutrient availability
Title: Tor Signaling Regulates Transcription of Amino Acid Permeases through a GATA Transcription Factor Gaf1 in Fission Yeast.

Data show that the newly synthesized glucose transport Ght5 locates preferentially at the cell tips with the aid of the target of rapamycin (TOR) complex 2 signaling.
Title: Mechanisms of expression and translocation of major fission yeast glucose transporters regulated by CaMKK/phosphatases, nuclear shuttling, and TOR.

These results suggest that nitrogen depletion decreases Tor2 activity through Tsc2, thereby promoting the surface expression of Agp3 from trans-Golgi/endosomes.
Title: Constitutive Tor2 Activity Promotes Retention of the Amino Acid Transporter Agp3 at Trans-Golgi/Endosomes in Fission Yeast.

Rapamycin inactivates Tor2 activity in the presence of caffeine.
Title: TORC1 of fission yeast is rapamycin-sensitive.

While mutated TORC1 and TORC2 displayed diminished kinase activity and FKBP12/Fkh1-dependent rapamycin sensitivity, their phenotypes were nearly opposite in mitosis.
Title: The reverse, but coordinated, roles of Tor2 (TORC1) and Tor1 (TORC2) kinases for growth, cell cycle and separase-mediated mitosis in Schizosaccharomyces pombe.

The following tor2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tor2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc99948	NM_001021266.3 Latest version!	Schizosaccharomyces pombe phosphatidylinositol kinase Tor2 (tor2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tor2 ( NM_001021266.3 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595359.2 Schizosaccharomyces pombe phosphatidylinositol kinase Tor2 (tor2), mRNA.

Price & Availability↑

CloneID	OSc99948
Clone ID Related Accession (Same CDS sequence)	NM_001021266.3
Accession Version	NM_001021266.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	phosphatidylinositol kinase Tor2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021266.2.

ATGAAAGAAT TTCCTGGGTT GAAAAGCAGG AATGAAGAAA TTCGCAATAA AGCGGCAAAT 
GATCTTTATG AATATGTTAT AGCTTATTCT CGAGAATTAT CGGGCGAAGC TTTAGTTCAG 
TTTAACAATG ATGTTAACAA GTACGTTTAT ACTTTAATTC ACAGTACCGA TCCACTCGAT 
CGTCTGGCAG GCGTAACTGC CATAAATCGT CTAATTGATT ATGAAGGAGA AGATACTACA 
AGGATTACTA GATTTGCAAA TTATTTGCGT ATTATTCTTC CAGGAACTGA CCAAAAAGCC 
ACCGTTCTTG CTGCAAAAGC TTTAGGGAGA CTTGCAGTTC CTGGAGGTGC TCTAACTTCT 
GAATTTGTGA ACTTCGAAGT TAAACGCGCT TTGGAATGGC TTCAAGGAGA ACGTAATGAA 
AATCGTAGAT ATGCAGCAGT GCTTATACTT AAGGAGCTTG CCAAAAATAC GTCTACATTA 
ATCTATGCTC ATATCGATTC AATTTTTGAG CTTTTATGGC ATGGACTTCG GGATCCAAAA 
GTTACTATTC GAATAGCTTC GGCTGATGCG TTGAGTGAGT TCTTGAAAAT CGTTAGGCAA 
AGGGATTCAT CCATCCGTCT CCAATGGTAT ACAAGTATTC TCAATGAAGC TCAACGTGGT 
GTTGCACAGG GTTCTTCTGA TTACATTCAT GGTAGTTTAC TAGTATATCG CCAATTATTT 
TTAAAAGCTG GAATGTTTAT GCATGAGAGG TATCGAGAAG TATCGGACAT TATATTGCAA 
TTCAGAGATC ACAAAGATTT GCTTATTCGC AAAACCGTTA CAGAATTGAT TGCTACACTC 
GCAGCTTATA ATCCAGATGA ATTTGTTTCC AATTATTTAC ACGTTTGTAT GCTCCATCTA 
CTTAATCTAT TAAAAAAAGA AAATGTTAAG ATGTTGGCGT TTGCCACTAT TGGAAAAGTT 
GCCGTGGCTA TTACTAATAG CATAATCCCA TATCTTGACC CCATATGCGA TTCAATCAAG 
GAAAGTCTAA AAATCCATAT CCGTAATAAA AGTGCTTCGG ATGCTGCAAT TTTTCAATGC 
ATTAGCCTAC TTTCTATAGC ACTAGGACAA GCCTTTTCAA ACTATGCGTA TGACCTCTTT 
GACTTAATAT TTGCAAGCGG ACTTTCTGAG GCTTCTTACC GTGCTCTCTC AGATCTGGCC 
CATAATATAC CTCCATTATT ACCAGTCATA CAGGAACGAC TATTAGATAT GCTCAGCAAA 
ATTCTAAGTG GTCGTCCTTT TATTCCTCCA GGATGTCCTC CCCAATATGT AGCAAGATCT 
TTAAAATCTT CAAAGAGTGC TTCCCTGAAG ACAGGATTTT TTCCCAATGA CGTCTATATT 
TTGGCTCTAA AAGTTTTGGG AAATTTTGAC TTCTCCGGTT ATATTCTTAA TGAATTTGTG 
AAGGATTGTG TAGTTGTATA CCTTGAAAAC AATGACCCGG AAGTACGCAA AACCGCCTCC 
ATAACCTGCT CCCAACTATT TGCTCGTGAT CCTATTTTAT CGCAGACTAG TGATCATGCG 
ATTCAAGTAG TTGCCGAAGT GCTAGAAAAG CTTCTAACAG TTGGTATTTG CGATACTGTT 
CCAGATATTC GGCTAACTGT TCTTAATTCT CTCGATTCTC GCTTTAACAA GCATTTAGCC 
CAAGCAGATA AAATTCGTTT ACTTTTTATT GCTATTAATG ATGAAAACTT TGCTGTACGA 
GAATCTGCAT TACGGATAAT TGGAAGACTA AATGTCTATA ACCCTGCCTA TGTGATGCCA 
TATTTAAGGA AAATAATGTT AAAAACTTTA ACAATATTGG ACTATTCTAC AATTATCAGA 
ACTAAAGAAG AGAATGCCAA GTTATTGTGT TTATTAATTG CAGCAGCTCC TCGCTTAATA 
GAGTCACATG TGGAACCAAT ACTGCAAATA CTTTTACCCA AAGCTAAAGA TTCTAGTTCA 
ATTGTGGCAG CTAGTATTGT TAATTCGCTT GGTGAAATAT GCCAAATCAG CGGTGAGGTT 
ATTGTTCCTT TTATTAAAGA CTTAATGCCG CTAATAATTG AGGCTTTGCA GGACCAAAGT 
TCTCCTATTA GGAGAGCAGC GGCTTTAAAG GCTTTGGGTA ATTTATCAAG CAGCACCGGA 
TATGTAATTG ATCCGTATAT TGAATTCCCT TCTTTACTTG ATATACTTAT TGGCATAACG 
AAAACCGAAC AAGATATTAC TATTCGTCGA GAGACTATTA AACTAATAGG TACACTTGGT 
GCTTTAGATC CCAATAGGCA TCGTGTTTTA GAAAAGGGAA CAGAAAAAGT AGTTCCAGAA 
CAGAAAAACA TTCCTCCTGA CATTTCTTTG CTGATGTCGG GCATTGGTCC ATCATCCGAT 
GAATATTACC CCACAGTCGT GATTACTGCT TTGATGAGTA TATTAAAAGA CCCGTCCTTA 
ACTATTCACC ATACTGCTGT TATTCAAGCT GTAATGTATA TATTCAAGAC CATGGGTCTC 
AGATGTGCCC CCTTTCTTTC CCAAATTATT CCAGAATTTA TCGCAGTTAT GAGAACGTGT 
CCTACGAACA TATTGGAGTT TTATTTTCAG CAACTAAGTA TTTTGGTCCT TATTGTTCGT 
CAGCACATAC GAAGTTTTTT ACCAGATTTA TTTAAGCTTA TCAAAGATTT TTGGAACCCG 
CATTCTAATT TACAATTTAC AATTTTGTCG CTAATTGAAT CACTGGCTAG AGCAATGCAG 
GGAGAGTTCA AGCCATACTT ACCATCTTTA TTAGTAATGA TGCTACAAAT ATTTGACTCT 
GATGTATCAG TGGACTCAGT CAGTACAAAA AAGGTATTGC ACGCATTCAT CGTCTTCGGT 
GATACATTAG CTGATTATTT TCATATGCTC TTGGATCCAA TACTTCGACT GTATGAGCGA 
AATGATGTTT CAATCGGTAT AAAAGAAAGT ATAATGATTA CAATTGGACG ACTTTCAATG 
GTTATTAATC TCTCGGAATA TGCATCAAGA ATTATACATC CTGTTATGCG TATGCTTTCG 
TGCAACAATG CCTCTCTCAT TCGCGTGAGT ATGGATACTG TATGTGCGCT TATATATCAA 
CTTAATGTTG ATTTTGCTAT TTTCATTCCA ATGATTGATA AGTGCCTTAA GATGAATGGC 
GTTACTCATG AAACCTACTC GATACTTGTT GAACAATTTT TACAAGAACA GCCTCTTCCT 
ATAAAACTCA ACCCTTATGA AAAATACGAT AAGCCTAAAT TAGATGTGGT AGCTTCCGCT 
GCTGATATAA CCTCTAAAAA GCTTCCTGTA AATCAAGAAA TCCTCAGAAA TGCGTGGGAA 
GCATCTCAAC GATCGACTAA GGATGACTGG CAAGAATGGA TCCGACGATT AGGCGTAGCT 
CTCTTAAGAG AATCACCTTC ACATGCCTTA CGAGCATGTG CTGCTTTGGC TGCTGCCTAT 
CAACCTTTAG CCAGGGACTT ATTTAACGCT AGTTTTGTTT CTTGTTGGTC TGAATTATAT 
GATCACTTTC AAGAAGAATT AGTTAAGTCG ATTGAGATAG CTTTAACGTC GCCACACATA 
TCTCCTGAAA TTATTCAGAT TTTACTCAAT CTTGCGGAAT TTATGGAGCA TGATGATAAA 
CCACTTCCGA TTGATATTCG TACTTTGGGT GCCTATGCAG CTAAATGCCA TGCGTTTGCT 
AAGGCTTTAC ATTACAAGGA GCTAGAATTC ATTGAAGAAG AATTGGTGAC AAAACCTTCG 
GTTGATACAA TTGAGGCCCT AATTTCAATC AATAATCAAC TCCAACAACC AGACGCAGCT 
ATTGGAATAC TTAAACATGC TCAGCAGCAT GACAAAATGA ATCTTAAAGA AACTTGGTAC 
GAAAAGTTAC AAAGGTGGGA AGATGCTCTG TCAGCTTATG AAAAAAGAGA AGCTGCTGGC 
GCTGGCAATT TCGAAATAAC GATGGGCAAA CTTCGTTGTT TACACGCTTT GGGTGAATGG 
GATCGATTAT CTCAGTTAGC TCAAGAAAAT TGGATTCATG CTGGTCATGA TGCGCGACGG 
TATATTGCAC CGCTTTCCGT AGCCGCTGCT TGGGGGTTAG GTCAATGGGA GCAAATGGAT 
GAATATATTT CTGTTATGAA GTCTGAGTCA CCAGATAAAG CTTTTTTTAA CGCGATTGTG 
GCTCTACATC GATCACAATT TGAAGAAGCA GCTTCTTATA TCACCAGAGC TCGAGATTTA 
CTTGATACTG AGCTTACTGC GCTTGTTGGT GAAAGTTATA ATAGGGCTTA CAGCGTTGCT 
GTCCGTGTTC AAATGTTATC CGAATTAGAA GAAATCATTA CCTATAAAAA AGCCGAGGAC 
AAACCCGAAG TTCGTGAAAT GATAAAGAAG ACATGGGTTA GACGTTTGAA AGGCTGTCAG 
AGAAATGTGG ATGTTTGGCA AAGAATGTTA AGAATACGAT CTTTAGTTAT TTCACCCCGT 
GATAACATGG AGATGTGGAT CAAATTCGCT AATCTCTGCA GAAAATCTGG TAGGATCAGT 
CTAGCGAAAA AATCACTTAA TTTACTCCTT GAAGACGACG AAAACTTAGA TAATTCACTC 
GTCTTAAAGA AGACTCACCC TTCAATTGTC TATGCAAATC TAAAGTTTTT GTGGGCTGTT 
GACGATAAAA GGAAGGCTCT TAATAGCATG CAAGAGTTTA CTTCTCAGCT AATTTCTGAC 
ATTAATGTTG ACCCTGCTCT TTTTGTTCAA TCTACATCAG TTAATACTCA AAAATCACAA 
GAAGAAATTC AATATTATTT TCATCTTCTA GCTAGATGTT ATCACAAGCA AGGTCAATGG 
CAGCAAGAAA TTGAGAATAA TTGGTCAGAA GGATCTTTTG ATGGAGTTCT TCAATCATAT 
ATGTATGCTA CGCAATTTGA TTCTAAATGG TATAAAGCTT GGCACTCTTG GGCATTAGCT 
AATTTCGAAG CTGTAAAGTT TTTGGAGCAA TCAGAGGAGC AAATCCCAAG TGCTGCATAT 
GAGCAGTATA TTATTCCAGC AGTCAAAGGC TTTTTTAAGT CAATTGCTTT AAGCAAAGGT 
AACCTTCAGG ACACCTTGCG CCTTCTTAAT CTTTGGTTTA AATTCGGCAA TAATAGCAAT 
GTAATTAATA CTTTGAACGT TGGTATATCC ACTGTGAACA TCGATATCTG GCTGGACGTT 
ATACCTCAGC TTATTGCGAG AATTCATGCC TCCTCTTTGA GTGTTCGTAA ATCTGTGCAT 
CAGTTGCTTT CCGATGTAGG TCGCGCGCAT CCCCAAGCTC TTGTTTATCC TTTAACTGTA 
GCAGCAAAAT CGCAGAGTTC TGCTAGGCAA AACGCTGCTT TAGCAATTAT GGATTCCTTG 
AAAACACATA GTCCACGTCT AGTTGAACAA GCAAGACTTG TTAGTCATGA GCTTATCAGA 
GCTGCAATCC TTTGGCATGA ACAATGGCAT GAAGGATTGG AAGAGGCTTC ACGATTGTAT 
TTTGGTGATC ATAACATTGA GGGTATGTTT GCCGTGTTGA GACCATTGCA TGAAATGTTG 
GAACGTGGTC CGGAAACGCT TCGTGAAATT TCTTTCCAAC AAGCCTTTGG ACGTGACTTA 
GTCGAAGCAA GGGATTGTTG TATCCGTTTT GAGCAAACTG GTGATATATC AGACTTAAAT 
CAGGCCTGGG ACTTATATTA CCAAGTTTTC AAGAAAATTC GTAAACAGCT ACCTCAGTTG 
ACAACATTAG ATTTACAATA CGTTTCTCCT AAACTCTTAC ATGTACATGA TCTTGAGTTA 
GCTGTTCCGG GAACATACGT TAGCGGGAAA CCTGTTATAA GAATTGTAAA GTTTTACCCT 
ACATTCAACG TGATAACATC TAAGCAACGT CCGAGACGAC TGAGTATTAA AGGCAGTGAT 
GGAAAAGATT ATCAATATGT TTTAAAGGGT CACGAGGACA TCAGACAAGA TGAACGTGTT 
ATGCAGCTAT TTGGGCTTTG TAACAATCTG TTACTAGCTG ACCCGGAAAC TTTTAAGCGC 
TTACTTTCAA TCCAAAGGTA TCCAGTGATT CCTTTATCAC CGGATTCAGG CCTTTTAGGC 
TGGGTTTTGG ATAGCGATAC TTTGCATGTC TTAATTCGTG ACTACAGAGA AAGCAGAAAG 
ATTTTACTCA ACATAGAACA TAGGTTGATC ATTCAGATGG CTCCAGATTA CGATCGTCTC 
ACTCTCCTTC AGAAAGTTGA AGTCTTTGAA TATGCCCTAC TTAGTACTAC GGGGCAGGAT 
TTATACCGCG TTTTATGGCT GAAGAGTCGT AGCTCAGAAG CTTGGCTTAA TAGGCGAACT 
AACTATTCTC GTACTCTTGC GGTGATGTCA ATGGTAGGGT ACATCTTAGG TTTGGGCGAT 
CGTCATCCTT CCAATTTGAT GCTGGACAGG TATACAGGTA ATATCATCCA TATCGATTTT 
GGAGATTGTT TTGAAGTAGC AATGCATCGA GAAAAGTTTC CCGAGAAGAT TCCTTTCCGC 
CTTACGCGAA TGCTTGTTAA CGCAATGGAA GTTAGTGGTA TAGAGGGGAC GTTCCGGATA 
ACATGCGAAC ATGTTATGCG AGTTCTTCGT ACTAATAAGG AGTCTGTGAT GGCAGTTTTA 
GAAGCTTTCG TTTACGATCC TCTTATTAAC TGGCGCCTTG CTCCTGCATA TTCACCTTCT 
ATCGATGAAA AGCAATCTAA CGAACCTAAT ACACTTCTCG GTGAGACAAT TGATGGTTTA 
CACCGGAAAC GCTTAAACGA AGAAGGAATT ACACTTGAAG AACGTCAAAA ACCCGAGATA 
CTCAATCAAA GAGCTATAAC CGTTCTTAAT CGTGTGTCCA ATAAACTTAC TGGTCGGGAT 
TTCAAACCCC AACAGCAATT GGATGTACCA AGTCAAGTAG AGAAGCTGAT TTTACAAGCA 
ACCTCTATAG AGAACCTATG CTTGTGCTAT ATTGGCTGGT GTAGTTTCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595359.2
CDS	164..7177
Translation	MKEFPGLKSRNEEIRNKAANDLYEYVIAYSRELSGEALVQFNNDVNKYVYTLIHSTDPLDRLAGVTAINRLIDYEGEDTTRITRFANYLRIILPGTDQKATVLAAKALGRLAVPGGALTSEFVNFEVKRALEWLQGERNENRRYAAVLILKELAKNTSTLIYAHIDSIFELLWHGLRDPKVTIRIASADALSEFLKIVRQRDSSIRLQWYTSILNEAQRGVAQGSSDYIHGSLLVYRQLFLKAGMFMHERYREVSDIILQFRDHKDLLIRKTVTELIATLAAYNPDEFVSNYLHVCMLHLLNLLKKENVKMLAFATIGKVAVAITNSIIPYLDPICDSIKESLKIHIRNKSASDAAIFQCISLLSIALGQAFSNYAYDLFDLIFASGLSEASYRALSDLAHNIPPLLPVIQERLLDMLSKILSGRPFIPPGCPPQYVARSLKSSKSASLKTGFFPNDVYILALKVLGNFDFSGYILNEFVKDCVVVYLENNDPEVRKTASITCSQLFARDPILSQTSDHAIQVVAEVLEKLLTVGICDTVPDIRLTVLNSLDSRFNKHLAQADKIRLLFIAINDENFAVRESALRIIGRLNVYNPAYVMPYLRKIMLKTLTILDYSTIIRTKEENAKLLCLLIAAAPRLIESHVEPILQILLPKAKDSSSIVAASIVNSLGEICQISGEVIVPFIKDLMPLIIEALQDQSSPIRRAAALKALGNLSSSTGYVIDPYIEFPSLLDILIGITKTEQDITIRRETIKLIGTLGALDPNRHRVLEKGTEKVVPEQKNIPPDISLLMSGIGPSSDEYYPTVVITALMSILKDPSLTIHHTAVIQAVMYIFKTMGLRCAPFLSQIIPEFIAVMRTCPTNILEFYFQQLSILVLIVRQHIRSFLPDLFKLIKDFWNPHSNLQFTILSLIESLARAMQGEFKPYLPSLLVMMLQIFDSDVSVDSVSTKKVLHAFIVFGDTLADYFHMLLDPILRLYERNDVSIGIKESIMITIGRLSMVINLSEYASRIIHPVMRMLSCNNASLIRVSMDTVCALIYQLNVDFAIFIPMIDKCLKMNGVTHETYSILVEQFLQEQPLPIKLNPYEKYDKPKLDVVASAADITSKKLPVNQEILRNAWEASQRSTKDDWQEWIRRLGVALLRESPSHALRACAALAAAYQPLARDLFNASFVSCWSELYDHFQEELVKSIEIALTSPHISPEIIQILLNLAEFMEHDDKPLPIDIRTLGAYAAKCHAFAKALHYKELEFIEEELVTKPSVDTIEALISINNQLQQPDAAIGILKHAQQHDKMNLKETWYEKLQRWEDALSAYEKREAAGAGNFEITMGKLRCLHALGEWDRLSQLAQENWIHAGHDARRYIAPLSVAAAWGLGQWEQMDEYISVMKSESPDKAFFNAIVALHRSQFEEAASYITRARDLLDTELTALVGESYNRAYSVAVRVQMLSELEEIITYKKAEDKPEVREMIKKTWVRRLKGCQRNVDVWQRMLRIRSLVISPRDNMEMWIKFANLCRKSGRISLAKKSLNLLLEDDENLDNSLVLKKTHPSIVYANLKFLWAVDDKRKALNSMQEFTSQLISDINVDPALFVQSTSVNTQKSQEEIQYYFHLLARCYHKQGQWQQEIENNWSEGSFDGVLQSYMYATQFDSKWYKAWHSWALANFEAVKFLEQSEEQIPSAAYEQYIIPAVKGFFKSIALSKGNLQDTLRLLNLWFKFGNNSNVINTLNVGISTVNIDIWLDVIPQLIARIHASSLSVRKSVHQLLSDVGRAHPQALVYPLTVAAKSQSSARQNAALAIMDSLKTHSPRLVEQARLVSHELIRAAILWHEQWHEGLEEASRLYFGDHNIEGMFAVLRPLHEMLERGPETLREISFQQAFGRDLVEARDCCIRFEQTGDISDLNQAWDLYYQVFKKIRKQLPQLTTLDLQYVSPKLLHVHDLELAVPGTYVSGKPVIRIVKFYPTFNVITSKQRPRRLSIKGSDGKDYQYVLKGHEDIRQDERVMQLFGLCNNLLLADPETFKRLLSIQRYPVIPLSPDSGLLGWVLDSDTLHVLIRDYRESRKILLNIEHRLIIQMAPDYDRLTLLQKVEVFEYALLSTTGQDLYRVLWLKSRSSEAWLNRRTNYSRTLAVMSMVGYILGLGDRHPSNLMLDRYTGNIIHIDFGDCFEVAMHREKFPEKIPFRLTRMLVNAMEVSGIEGTFRITCEHVMRVLRTNKESVMAVLEAFVYDPLINWRLAPAYSPSIDEKQSNEPNTLLGETIDGLHRKRLNEEGITLEERQKPEILNQRAITVLNRVSNKLTGRDFKPQQQLDVPSQVEKLILQATSIENLCLCYIGWCSFW

Target ORF information:

RefSeq Version	NM_001021266.3
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe phosphatidylinositol kinase Tor2 (tor2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021266.3

ORF Insert Sequence:

ATGAAAGAAT TTCCTGGGTT GAAAAGCAGG AATGAAGAAA TTCGCAATAA AGCGGCAAAT 
GATCTTTATG AATATGTTAT AGCTTATTCT CGAGAATTAT CGGGCGAAGC TTTAGTTCAG 
TTTAACAATG ATGTTAACAA GTACGTTTAT ACTTTAATTC ACAGTACCGA TCCACTCGAT 
CGTCTGGCAG GCGTAACTGC CATAAATCGT CTAATTGATT ATGAAGGAGA AGATACTACA 
AGGATTACTA GATTTGCAAA TTATTTGCGT ATTATTCTTC CAGGAACTGA CCAAAAAGCC 
ACCGTTCTTG CTGCAAAAGC TTTAGGGAGA CTTGCAGTTC CTGGAGGTGC TCTAACTTCT 
GAATTTGTGA ACTTCGAAGT TAAACGCGCT TTGGAATGGC TTCAAGGAGA ACGTAATGAA 
AATCGTAGAT ATGCAGCAGT GCTTATACTT AAGGAGCTTG CCAAAAATAC GTCTACATTA 
ATCTATGCTC ATATCGATTC AATTTTTGAG CTTTTATGGC ATGGACTTCG GGATCCAAAA 
GTTACTATTC GAATAGCTTC GGCTGATGCG TTGAGTGAGT TCTTGAAAAT CGTTAGGCAA 
AGGGATTCAT CCATCCGTCT CCAATGGTAT ACAAGTATTC TCAATGAAGC TCAACGTGGT 
GTTGCACAGG GTTCTTCTGA TTACATTCAT GGTAGTTTAC TAGTATATCG CCAATTATTT 
TTAAAAGCTG GAATGTTTAT GCATGAGAGG TATCGAGAAG TATCGGACAT TATATTGCAA 
TTCAGAGATC ACAAAGATTT GCTTATTCGC AAAACCGTTA CAGAATTGAT TGCTACACTC 
GCAGCTTATA ATCCAGATGA ATTTGTTTCC AATTATTTAC ACGTTTGTAT GCTCCATCTA 
CTTAATCTAT TAAAAAAAGA AAATGTTAAG ATGTTGGCGT TTGCCACTAT TGGAAAAGTT 
GCCGTGGCTA TTACTAATAG CATAATCCCA TATCTTGACC CCATATGCGA TTCAATCAAG 
GAAAGTCTAA AAATCCATAT CCGTAATAAA AGTGCTTCGG ATGCTGCAAT TTTTCAATGC 
ATTAGCCTAC TTTCTATAGC ACTAGGACAA GCCTTTTCAA ACTATGCGTA TGACCTCTTT 
GACTTAATAT TTGCAAGCGG ACTTTCTGAG GCTTCTTACC GTGCTCTCTC AGATCTGGCC 
CATAATATAC CTCCATTATT ACCAGTCATA CAGGAACGAC TATTAGATAT GCTCAGCAAA 
ATTCTAAGTG GTCGTCCTTT TATTCCTCCA GGATGTCCTC CCCAATATGT AGCAAGATCT 
TTAAAATCTT CAAAGAGTGC TTCCCTGAAG ACAGGATTTT TTCCCAATGA CGTCTATATT 
TTGGCTCTAA AAGTTTTGGG AAATTTTGAC TTCTCCGGTT ATATTCTTAA TGAATTTGTG 
AAGGATTGTG TAGTTGTATA CCTTGAAAAC AATGACCCGG AAGTACGCAA AACCGCCTCC 
ATAACCTGCT CCCAACTATT TGCTCGTGAT CCTATTTTAT CGCAGACTAG TGATCATGCG 
ATTCAAGTAG TTGCCGAAGT GCTAGAAAAG CTTCTAACAG TTGGTATTTG CGATACTGTT 
CCAGATATTC GGCTAACTGT TCTTAATTCT CTCGATTCTC GCTTTAACAA GCATTTAGCC 
CAAGCAGATA AAATTCGTTT ACTTTTTATT GCTATTAATG ATGAAAACTT TGCTGTACGA 
GAATCTGCAT TACGGATAAT TGGAAGACTA AATGTCTATA ACCCTGCCTA TGTGATGCCA 
TATTTAAGGA AAATAATGTT AAAAACTTTA ACAATATTGG ACTATTCTAC AATTATCAGA 
ACTAAAGAAG AGAATGCCAA GTTATTGTGT TTATTAATTG CAGCAGCTCC TCGCTTAATA 
GAGTCACATG TGGAACCAAT ACTGCAAATA CTTTTACCCA AAGCTAAAGA TTCTAGTTCA 
ATTGTGGCAG CTAGTATTGT TAATTCGCTT GGTGAAATAT GCCAAATCAG CGGTGAGGTT 
ATTGTTCCTT TTATTAAAGA CTTAATGCCG CTAATAATTG AGGCTTTGCA GGACCAAAGT 
TCTCCTATTA GGAGAGCAGC GGCTTTAAAG GCTTTGGGTA ATTTATCAAG CAGCACCGGA 
TATGTAATTG ATCCGTATAT TGAATTCCCT TCTTTACTTG ATATACTTAT TGGCATAACG 
AAAACCGAAC AAGATATTAC TATTCGTCGA GAGACTATTA AACTAATAGG TACACTTGGT 
GCTTTAGATC CCAATAGGCA TCGTGTTTTA GAAAAGGGAA CAGAAAAAGT AGTTCCAGAA 
CAGAAAAACA TTCCTCCTGA CATTTCTTTG CTGATGTCGG GCATTGGTCC ATCATCCGAT 
GAATATTACC CCACAGTCGT GATTACTGCT TTGATGAGTA TATTAAAAGA CCCGTCCTTA 
ACTATTCACC ATACTGCTGT TATTCAAGCT GTAATGTATA TATTCAAGAC CATGGGTCTC 
AGATGTGCCC CCTTTCTTTC CCAAATTATT CCAGAATTTA TCGCAGTTAT GAGAACGTGT 
CCTACGAACA TATTGGAGTT TTATTTTCAG CAACTAAGTA TTTTGGTCCT TATTGTTCGT 
CAGCACATAC GAAGTTTTTT ACCAGATTTA TTTAAGCTTA TCAAAGATTT TTGGAACCCG 
CATTCTAATT TACAATTTAC AATTTTGTCG CTAATTGAAT CACTGGCTAG AGCAATGCAG 
GGAGAGTTCA AGCCATACTT ACCATCTTTA TTAGTAATGA TGCTACAAAT ATTTGACTCT 
GATGTATCAG TGGACTCAGT CAGTACAAAA AAGGTATTGC ACGCATTCAT CGTCTTCGGT 
GATACATTAG CTGATTATTT TCATATGCTC TTGGATCCAA TACTTCGACT GTATGAGCGA 
AATGATGTTT CAATCGGTAT AAAAGAAAGT ATAATGATTA CAATTGGACG ACTTTCAATG 
GTTATTAATC TCTCGGAATA TGCATCAAGA ATTATACATC CTGTTATGCG TATGCTTTCG 
TGCAACAATG CCTCTCTCAT TCGCGTGAGT ATGGATACTG TATGTGCGCT TATATATCAA 
CTTAATGTTG ATTTTGCTAT TTTCATTCCA ATGATTGATA AGTGCCTTAA GATGAATGGC 
GTTACTCATG AAACCTACTC GATACTTGTT GAACAATTTT TACAAGAACA GCCTCTTCCT 
ATAAAACTCA ACCCTTATGA AAAATACGAT AAGCCTAAAT TAGATGTGGT AGCTTCCGCT 
GCTGATATAA CCTCTAAAAA GCTTCCTGTA AATCAAGAAA TCCTCAGAAA TGCGTGGGAA 
GCATCTCAAC GATCGACTAA GGATGACTGG CAAGAATGGA TCCGACGATT AGGCGTAGCT 
CTCTTAAGAG AATCACCTTC ACATGCCTTA CGAGCATGTG CTGCTTTGGC TGCTGCCTAT 
CAACCTTTAG CCAGGGACTT ATTTAACGCT AGTTTTGTTT CTTGTTGGTC TGAATTATAT 
GATCACTTTC AAGAAGAATT AGTTAAGTCG ATTGAGATAG CTTTAACGTC GCCACACATA 
TCTCCTGAAA TTATTCAGAT TTTACTCAAT CTTGCGGAAT TTATGGAGCA TGATGATAAA 
CCACTTCCGA TTGATATTCG TACTTTGGGT GCCTATGCAG CTAAATGCCA TGCGTTTGCT 
AAGGCTTTAC ATTACAAGGA GCTAGAATTC ATTGAAGAAG AATTGGTGAC AAAACCTTCG 
GTTGATACAA TTGAGGCCCT AATTTCAATC AATAATCAAC TCCAACAACC AGACGCAGCT 
ATTGGAATAC TTAAACATGC TCAGCAGCAT GACAAAATGA ATCTTAAAGA AACTTGGTAC 
GAAAAGTTAC AAAGGTGGGA AGATGCTCTG TCAGCTTATG AAAAAAGAGA AGCTGCTGGC 
GCTGGCAATT TCGAAATAAC GATGGGCAAA CTTCGTTGTT TACACGCTTT GGGTGAATGG 
GATCGATTAT CTCAGTTAGC TCAAGAAAAT TGGATTCATG CTGGTCATGA TGCGCGACGG 
TATATTGCAC CGCTTTCCGT AGCCGCTGCT TGGGGGTTAG GTCAATGGGA GCAAATGGAT 
GAATATATTT CTGTTATGAA GTCTGAGTCA CCAGATAAAG CTTTTTTTAA CGCGATTGTG 
GCTCTACATC GATCACAATT TGAAGAAGCA GCTTCTTATA TCACCAGAGC TCGAGATTTA 
CTTGATACTG AGCTTACTGC GCTTGTTGGT GAAAGTTATA ATAGGGCTTA CAGCGTTGCT 
GTCCGTGTTC AAATGTTATC CGAATTAGAA GAAATCATTA CCTATAAAAA AGCCGAGGAC 
AAACCCGAAG TTCGTGAAAT GATAAAGAAG ACATGGGTTA GACGTTTGAA AGGCTGTCAG 
AGAAATGTGG ATGTTTGGCA AAGAATGTTA AGAATACGAT CTTTAGTTAT TTCACCCCGT 
GATAACATGG AGATGTGGAT CAAATTCGCT AATCTCTGCA GAAAATCTGG TAGGATCAGT 
CTAGCGAAAA AATCACTTAA TTTACTCCTT GAAGACGACG AAAACTTAGA TAATTCACTC 
GTCTTAAAGA AGACTCACCC TTCAATTGTC TATGCAAATC TAAAGTTTTT GTGGGCTGTT 
GACGATAAAA GGAAGGCTCT TAATAGCATG CAAGAGTTTA CTTCTCAGCT AATTTCTGAC 
ATTAATGTTG ACCCTGCTCT TTTTGTTCAA TCTACATCAG TTAATACTCA AAAATCACAA 
GAAGAAATTC AATATTATTT TCATCTTCTA GCTAGATGTT ATCACAAGCA AGGTCAATGG 
CAGCAAGAAA TTGAGAATAA TTGGTCAGAA GGATCTTTTG ATGGAGTTCT TCAATCATAT 
ATGTATGCTA CGCAATTTGA TTCTAAATGG TATAAAGCTT GGCACTCTTG GGCATTAGCT 
AATTTCGAAG CTGTAAAGTT TTTGGAGCAA TCAGAGGAGC AAATCCCAAG TGCTGCATAT 
GAGCAGTATA TTATTCCAGC AGTCAAAGGC TTTTTTAAGT CAATTGCTTT AAGCAAAGGT 
AACCTTCAGG ACACCTTGCG CCTTCTTAAT CTTTGGTTTA AATTCGGCAA TAATAGCAAT 
GTAATTAATA CTTTGAACGT TGGTATATCC ACTGTGAACA TCGATATCTG GCTGGACGTT 
ATACCTCAGC TTATTGCGAG AATTCATGCC TCCTCTTTGA GTGTTCGTAA ATCTGTGCAT 
CAGTTGCTTT CCGATGTAGG TCGCGCGCAT CCCCAAGCTC TTGTTTATCC TTTAACTGTA 
GCAGCAAAAT CGCAGAGTTC TGCTAGGCAA AACGCTGCTT TAGCAATTAT GGATTCCTTG 
AAAACACATA GTCCACGTCT AGTTGAACAA GCAAGACTTG TTAGTCATGA GCTTATCAGA 
GCTGCAATCC TTTGGCATGA ACAATGGCAT GAAGGATTGG AAGAGGCTTC ACGATTGTAT 
TTTGGTGATC ATAACATTGA GGGTATGTTT GCCGTGTTGA GACCATTGCA TGAAATGTTG 
GAACGTGGTC CGGAAACGCT TCGTGAAATT TCTTTCCAAC AAGCCTTTGG ACGTGACTTA 
GTCGAAGCAA GGGATTGTTG TATCCGTTTT GAGCAAACTG GTGATATATC AGACTTAAAT 
CAGGCCTGGG ACTTATATTA CCAAGTTTTC AAGAAAATTC GTAAACAGCT ACCTCAGTTG 
ACAACATTAG ATTTACAATA CGTTTCTCCT AAACTCTTAC ATGTACATGA TCTTGAGTTA 
GCTGTTCCGG GAACATACGT TAGCGGGAAA CCTGTTATAA GAATTGTAAA GTTTTACCCT 
ACATTCAACG TGATAACATC TAAGCAACGT CCGAGACGAC TGAGTATTAA AGGCAGTGAT 
GGAAAAGATT ATCAATATGT TTTAAAGGGT CACGAGGACA TCAGACAAGA TGAACGTGTT 
ATGCAGCTAT TTGGGCTTTG TAACAATCTG TTACTAGCTG ACCCGGAAAC TTTTAAGCGC 
TTACTTTCAA TCCAAAGGTA TCCAGTGATT CCTTTATCAC CGGATTCAGG CCTTTTAGGC 
TGGGTTTTGG ATAGCGATAC TTTGCATGTC TTAATTCGTG ACTACAGAGA AAGCAGAAAG 
ATTTTACTCA ACATAGAACA TAGGTTGATC ATTCAGATGG CTCCAGATTA CGATCGTCTC 
ACTCTCCTTC AGAAAGTTGA AGTCTTTGAA TATGCCCTAC TTAGTACTAC GGGGCAGGAT 
TTATACCGCG TTTTATGGCT GAAGAGTCGT AGCTCAGAAG CTTGGCTTAA TAGGCGAACT 
AACTATTCTC GTACTCTTGC GGTGATGTCA ATGGTAGGGT ACATCTTAGG TTTGGGCGAT 
CGTCATCCTT CCAATTTGAT GCTGGACAGG TATACAGGTA ATATCATCCA TATCGATTTT 
GGAGATTGTT TTGAAGTAGC AATGCATCGA GAAAAGTTTC CCGAGAAGAT TCCTTTCCGC 
CTTACGCGAA TGCTTGTTAA CGCAATGGAA GTTAGTGGTA TAGAGGGGAC GTTCCGGATA 
ACATGCGAAC ATGTTATGCG AGTTCTTCGT ACTAATAAGG AGTCTGTGAT GGCAGTTTTA 
GAAGCTTTCG TTTACGATCC TCTTATTAAC TGGCGCCTTG CTCCTGCATA TTCACCTTCT 
ATCGATGAAA AGCAATCTAA CGAACCTAAT ACACTTCTCG GTGAGACAAT TGATGGTTTA 
CACCGGAAAC GCTTAAACGA AGAAGGAATT ACACTTGAAG AACGTCAAAA ACCCGAGATA 
CTCAATCAAA GAGCTATAAC CGTTCTTAAT CGTGTGTCCA ATAAACTTAC TGGTCGGGAT 
TTCAAACCCC AACAGCAATT GGATGTACCA AGTCAAGTAG AGAAGCTGAT TTTACAAGCA 
ACCTCTATAG AGAACCTATG CTTGTGCTAT ATTGGCTGGT GTAGTTTCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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tor2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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