pcf1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following pcf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pcf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100059 NM_001021958.3
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Schizosaccharomyces pombe CAF assembly factor (CAF-1) complex large subunit Pcf1 (pcf1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc100059
    Clone ID Related Accession (Same CDS sequence) NM_001021958.3
    Accession Version NM_001021958.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product CAF assembly factor (CAF-1) complex large subunit Pcf1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021958.2.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAATAGTG AAAGTGTTGA TTCAGATGTC GCGGCCTCTA CTAGTAATAA GGGAAATGAG 
    TTATGTAGCT CTTCCACTGA CATTACGTCT TTATCAGTGA GTTCCCCGAA TGAATCGGTT
    ATTCATTCCA GTCACTCTGC AAGTGAAGCT GATGAGTACG TTTGCAAATT AAGTTATGAG
    GGTAATAGAA AGAAAAGAAT TTATAATGGC TCTGCTGAAG CGGGTAAAGA AAAGAAGCTT
    CAAAAACAGC GGGCCCAAGA GGAAAGGATT CGACAAAAAG AAGCAGAGCG TTTAAAACGT
    GAAAAGGAAC GTCAACAACG AGAGCAAGAG AAAAAGCTAA GAGAACAGGA AAAAATCGCC
    GCTAAGAAAA TGAAAGAATT AGAGAAATTA GAGAAGGAGC GGATTCGATT GCAGGAACAA
    CAAAGGCGGA AGGAGGAGAG GGATCAAAAG CTTCGTGAGA AAGAAGAAGC ACAGCGCCTT
    CGTCAAGAAC AAATCCTTAA TAAGGAAAGA CAACAATTGA AGTTAAACAA CTTTTTCACC
    AAAGGAGTTG AGAAACGAAT CGCGCCAAAC GAAAATTTTG TAGCTGACAA AACGGATGAA
    CTGAATGAGT TTGAGAAAGA GTTTCGCCCT TTTTTTATAA AGCATCAGAT GTCCCTTTCC
    AAATATCCAT CTCCGAACGA ATCTGATTCT TTTTTGGATG AAGTCCTCTC AACCTCTAAA
    TCTTATCCTT TAAAGCTAAA CGACATTTTT ACACCATCAG ATGCTGTAAG CTCCGCAAAC
    AGTTTAGGTG TTAGTAATAG AAATTCCGAA AACGAGGTTC GACAATTAAT GAGCGCATAT
    CAAGATCCAT CTGTTTCAAA ACCTCAAGAA ATTTTATCTT GTTTATCACA GATTCCTATT
    AAATTCATTT TCTTTTATCA GGATGTTCGC CCTCCATATT TTGGATCTTA CACGAAAACC
    CATTCCCATG GTTCTAACGT TTTATTGAAT CCCTGGCTTG AAGATGAAGA CATAGACTAC
    ACATATGATT CAGAAGCAGA ATGGGTAGCT GATGAGGAAG ATGATGGAGA AGATTTAGAA
    AGTGAAGATG AAGAGGTAGA TAATTCCGAT GATATTGTAG AGGATGGTGA TAATGCTTTT
    GTGGATGATG AGGATGATGA CAAGGATAGT GTCAATGCTT CTAATACCCA TCGTTCCTCC
    GGCCCATTAG AGGTAATCGT AGAAGGTCCG GTTTGGGATT CAAAGTTTCT TCCTGACTTT
    AATTGCTTAT CTTTAATTGA ACCCATTAGT TCATTTTCAG CATCTACATA TCTCCAAATT
    GATCCTAAAG AGGATTTATG GGCTTCTCAA GATACTGCTC CAGCGTCTAG TGGAATGACA
    ATTGGACCTA CCAGTTCACT TTCTGATGAT TTACAAGTAA GGTTTCCTTC TGAAGATATA
    CCCAAATTTA TAGAATATGT CCGTAACTCT CATGACAACA AAGTATTCCT GATCGAGAAT
    TTGAGGCACA TGTTTCCTTA CGTAACCAAA AATATTATCA GCGAAACTCT TGGAAAAGTC
    GCTGTGCGTA AAGGAAAATC AGTATCTGAT GGTTGGATAA TCAAGGAGAA TTTTGCATCC
    CTTTTATCCT CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_596048.2
    CDS152..1786
    Translation

    Target ORF information:

    RefSeq Version NM_001021958.3
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe CAF assembly factor (CAF-1) complex large subunit Pcf1 (pcf1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001021958.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAATAGTG AAAGTGTTGA TTCAGATGTC GCGGCCTCTA CTAGTAATAA GGGAAATGAG 
    TTATGTAGCT CTTCCACTGA CATTACGTCT TTATCAGTGA GTTCCCCGAA TGAATCGGTT
    ATTCATTCCA GTCACTCTGC AAGTGAAGCT GATGAGTACG TTTGCAAATT AAGTTATGAG
    GGTAATAGAA AGAAAAGAAT TTATAATGGC TCTGCTGAAG CGGGTAAAGA AAAGAAGCTT
    CAAAAACAGC GGGCCCAAGA GGAAAGGATT CGACAAAAAG AAGCAGAGCG TTTAAAACGT
    GAAAAGGAAC GTCAACAACG AGAGCAAGAG AAAAAGCTAA GAGAACAGGA AAAAATCGCC
    GCTAAGAAAA TGAAAGAATT AGAGAAATTA GAGAAGGAGC GGATTCGATT GCAGGAACAA
    CAAAGGCGGA AGGAGGAGAG GGATCAAAAG CTTCGTGAGA AAGAAGAAGC ACAGCGCCTT
    CGTCAAGAAC AAATCCTTAA TAAGGAAAGA CAACAATTGA AGTTAAACAA CTTTTTCACC
    AAAGGAGTTG AGAAACGAAT CGCGCCAAAC GAAAATTTTG TAGCTGACAA AACGGATGAA
    CTGAATGAGT TTGAGAAAGA GTTTCGCCCT TTTTTTATAA AGCATCAGAT GTCCCTTTCC
    AAATATCCAT CTCCGAACGA ATCTGATTCT TTTTTGGATG AAGTCCTCTC AACCTCTAAA
    TCTTATCCTT TAAAGCTAAA CGACATTTTT ACACCATCAG ATGCTGTAAG CTCCGCAAAC
    AGTTTAGGTG TTAGTAATAG AAATTCCGAA AACGAGGTTC GACAATTAAT GAGCGCATAT
    CAAGATCCAT CTGTTTCAAA ACCTCAAGAA ATTTTATCTT GTTTATCACA GATTCCTATT
    AAATTCATTT TCTTTTATCA GGATGTTCGC CCTCCATATT TTGGATCTTA CACGAAAACC
    CATTCCCATG GTTCTAACGT TTTATTGAAT CCCTGGCTTG AAGATGAAGA CATAGACTAC
    ACATATGATT CAGAAGCAGA ATGGGTAGCT GATGAGGAAG ATGATGGAGA AGATTTAGAA
    AGTGAAGATG AAGAGGTAGA TAATTCCGAT GATATTGTAG AGGATGGTGA TAATGCTTTT
    GTGGATGATG AGGATGATGA CAAGGATAGT GTCAATGCTT CTAATACCCA TCGTTCCTCC
    GGCCCATTAG AGGTAATCGT AGAAGGTCCG GTTTGGGATT CAAAGTTTCT TCCTGACTTT
    AATTGCTTAT CTTTAATTGA ACCCATTAGT TCATTTTCAG CATCTACATA TCTCCAAATT
    GATCCTAAAG AGGATTTATG GGCTTCTCAA GATACTGCTC CAGCGTCTAG TGGAATGACA
    ATTGGACCTA CCAGTTCACT TTCTGATGAT TTACAAGTAA GGTTTCCTTC TGAAGATATA
    CCCAAATTTA TAGAATATGT CCGTAACTCT CATGACAACA AAGTATTCCT GATCGAGAAT
    TTGAGGCACA TGTTTCCTTA CGTAACCAAA AATATTATCA GCGAAACTCT TGGAAAAGTC
    GCTGTGCGTA AAGGAAAATC AGTATCTGAT GGTTGGATAA TCAAGGAGAA TTTTGCATCC
    CTTTTATCCT CTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L