vps13a cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	vps13a
Entrez Gene ID	2540516
Full Name	chorein homolog (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001713095.2	XP_001713147.1	vacuolar protein sorting-associated protein
NM_001355826.1	NP_001342827.1	vacuolar protein sorting-associated protein

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following vps13a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the vps13a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100148	NM_001355826.1 Latest version!	Schizosaccharomyces pombe vacuolar protein sorting-associated protein (vps13a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OSj01810	XM_001713095.2 Latest version!	Schizosaccharomyces pombe 972h- vacuolar protein sorting-associated protein (vps13a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

vps13a ( NM_001355826.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342827.1 Schizosaccharomyces pombe vacuolar protein sorting-associated protein (vps13a), mRNA.

Price & Availability↑

CloneID	OSc100148
Clone ID Related Accession (Same CDS sequence)	NM_001355826.1
Accession Version	NM_001355826.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	vacuolar protein sorting-associated protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423).

ATGTTAGAAG GCCTAGTTGC CGGCCTCTTG AACAAAATTT TAGGTTCGTA TGTTGACAAC 
TTGGATACTA AACAACTAAA CATCGGCGTT TGGGGTGGCC ATGTTAGTTT GCATAATTTA 
CGAATAAAAC CTGAGGCCTT AGATAAACTC GGAATTCCCA TTGAAATCAC TAGTGGTTTA 
ATTGGCACGT TTACGCTGGA AATTCCATGG TCGAATCTTC GGAACAAATC TTTAACGATC 
AATATTGAAG ACATATATTT GTCAATTCAT CCCCAAGCGA AAAACTCTTT AACACGCGAT 
GAGTTGGAGC AATCTCAACA AGCTTTAAAG CAGGAACAGC TGGATAGTTT TGAAATCTTA 
CGGAAAAATT TTCGAGAAAC TTTAGAAGAG TCTTCATCAA ATCCAAATAT AAGTAGAAAA 
CAATCTTTTA TTGAGTATCT TATTGCCAAA CTTACTGATA ACATTCAAAT ATACATCGAA 
AGAATTCACT TGCGGTTTGA AGATAATTTG AGTGATCTTG AAAAACCTTA TTCTCTTGGA 
TTGACGCTTT ATTCGTTAAG AGTAACCTCT ACAGATGCTA GTTTTACCGA ATATTTGCTT 
TCAACCGATC CAATTCCTTC CTCTTGCATT CATAAAATTA TTACTGTTGA TTATTTCTCA 
ATATACTGGA TAAGTAAGTG TGAAATTTCT AAATGCACTA CTACAGAAGA CATATTCTCT 
TATTTAAAGA ACCTGATTCC TAGTGCAGAA AAGTCACCTG CTTATAATTA CATTTTAAAG 
CCGCTACGGG CAACCGCCCA TGTTGTCTTA TTTCGACATC CGACAGACCA GATAATGCAA 
TTAAGGGGAA AATTATCTGT TGAAGAAATA TCAATTACGC TAAGTGATCA TATGTATTAC 
AGTTTACTTG GTGTTATTGA CTACTTTCGT GTTGTAATGA AGCAACAATA TTATCTTCAA 
TATAGGCCTA AATCGACTCC TAAAGAGAAA CCATTGGAGT GGTTTAAGTA TGCCATTTTA 
GTTGTTAAAG ATTCTGTTCA TGAGAGCAGA TATCACTGGA CTTGGAAATA CTTTAAAAGG 
AGAAGGGACG ACAGAATAGC TTACATGCAT ATTATTCGTA AGAGGTATTT GAATGAGCAA 
ATATCAAAGG AAGAAATTGA TTTGCAAAAA AAGATAGAGA AACGAAATTC TACTTACGAT 
CTAATTAAGT ACCGTTCACG TGTGCATACA TCTCTGATTG AGGAAAGGAA TTCTATTTAT 
TTAAAACCAA AAACTTCGGC AGCTCATGGA TTGTACGATT GGTTTTCTGG GTATATACGA 
AAGCCGCAGT CCCAGGATGA AGATACATTA GCAAGTACTG ATAAAACAGC CGCTGACTTG 
ACTGATCAAG AACAGAAAGA ATTTTTTTCT GCAATAGAAT GGAGCGGACA ATTATATCCT 
GATACCGTAA ATTTAGATCC GGATATGTGT ATGGCTAATG TTGAAGTCTC TATCGCTAAA 
GGAAGTTTTG TCATACAATC TCACATTAAT GGACGAGTAA TACCTTTAAT TAAGCAAAGG 
TTTGAGAGTT TTGCAACAGA ATGTTTTATA AGGCCTCAAT CACTGAAATT GAAAGTGTCT 
CTTAAGGATT TGGATATGTT TGATGGCATA ACGAACCCCG AGCTTGAGCC TGCTCGTGTA 
ATTTTTGCAA AGCCTTCTGT TGAAGAAAGT GAAAGTTTGC AAAAAATCCC TGAAGCCTAC 
AGAACACATT TGTTTTTTTT ATTGTTAGAC ACCAAACCGG TGTATAAAGC GAGTTCTACT 
CTAATCGTAC ATTTGCGAAC TTTGGTTATT ATTTACAATC GAGTTTGTAT TGAAAGTCTG 
CTAGCTTTTT TTGTTCCTCC AAGAACAAAA ATCGAGCACG TTTCTGAATG GGGATATTCT 
GCAGCTGCAA AAGTTATGAC CCTAGCTAGA CAAACTCGTG CTAGTTTGGA TTATGCCCTA 
GAAATGCACA AGACTTCCGA TATGACAATT GACCTGCAAG CTCCCTTGAT TGTTGTTCGA 
GAAGAATGTA CGGATTTAAA AAGCCCTACA CTGTTCCTTG ACGTGGGTAG AGCGTTGGTA 
CATACCCAAT TAGTTGATGA TGCGATTATC GACAAATTCA GAAAACTCCA GAGCAAAAAG 
ATAAACAACG AGCAATTAAA ACAACTAGAA AACTTGATGT ACGACAAGTT TACCATCTCA 
TTATTTAATG TTCGATGCTT AATTGGGCCT GATTATGAAA CTGGTTGGCG TTGCTTACCC 
AAAGGGTGTG ATTACCATAT TTTGAAAGAA TGCTCTCTTG ACATTAACTT TGAAATATCA 
ATCCTTCAAA AAGCTACTAA CTTAACGAAG TTCAAAGTTT CAAGTCATAT GAAGCATGCC 
GAAATTATGT TTTCTGATGT GCAATATAAG GTATTCATTA ATATGATGAG CAATATATTG 
CCTACGCTTC CTGTTGCTGA AATACCATTT ACATACCAAC AATTCCTTGA CGCGGTTAAG 
CCTCCTCCTT TCTTTGATGC TCCTGATAAT TTTCAAATAA CTCATACTTC TCTTGGAAGT 
CATGCTAATG AAAACACCGC TGCGCAATTT ATGGCCCAGC AAATTTTTGC TTTCTATTTT 
AAGGTGGATT ATGCTATATG TTCGTTGTAC CGAAGATCTG AAAACTATCT CATACCTGTG 
GTAAGAGCAT TTACAGAGTT TTACATCGAC CTAGTAGTGC GAAAATTTGA TTACCTTGTT 
ACTTCCAAGC TTAACGACTT GGTTATTAAA GAATTTACAT ATCCTTCTTC TCTTTGTGAC 
AATGTTTTAG TTCGTTCTTC TCCTTCACCT AAAAATAACT TTGATGATAC CGTTTTCATC 
AGCTATACAA GCATTGATTA TGACTCTCCT GAACTAGATT CTGTTTACGA AGGTGTTCGA 
ACTACCATTG CTGTAGTCCT TTCTGATCTC ATTCTCAACG TTGAGCCTAC TGGATTTTCA 
TTTGTCTACG ATTTTATCAG AGCCACTTTT ACCTCTTTAA ATGATGAGTA TATGATTGGT 
GAAGATCCAG AGTTGACTCG AAAAATTTCA CCAGTTGAAG GAATTATTCC AGAAGACGCC 
AACGTTCGGT TTGACAACGT AGATATTTTT CTTTATGACT GTGATCAACA TTTTTCAACA 
GTTTGTCTGT ATAGTGCTAA CATGCATATG GAATTCAGAG AGAAGTTTTT CCTTCAAGCA 
CGATTTTATG ACCTTGAAGT TAAAAACCAT ATGAAAAGTA ATAATCCGCC AAAGACCATT 
GTGAAGATTG ACGATAATGA TTTATTTATA TTTAAATATG AGAGTTATGA CATTCCAAAA 
GACATATCGA AACCTACATG CGACTGTGTT TATGATATAT CTTTTGGCTC ATTAACTTTT 
TATTTTCAAA AATCATATTT CAATGCAATA TACGATTTTT TATTAAAGTT GAAGCGATTT 
CAGGAATTAT TTAGCTCAAT TAGATATGCG ATCTACTATA AGCTTTACGG AAACAAAGTT 
AGCCTAACTT ATCCTAAATT TGAGCTTCGA ATTAAGCATC CAAAGGTATA CTTTGATGAT 
GTATTAGATG AAGAAAGAAA TTGTCGGATG CAATTGATAG TGAAACCCCA ATCCTTTTAT 
GCGTTTAGTA AATGTCCGAT TGTGGAAAAG AACTCGAAGA AATCAATATT CAGTTGTGAA 
ATAACTAAGG TTGAATTTCA TACTGCCGTC CCTAGTTCCT CGCATCACGA TGTACTAATG 
GAGGAAAATA ATGTTCATTT GGACCTTACA TATGACGCTA ATTACACTAC TGGTGCTTAT 
GTATTCAAAG CAACTGGTGA TTTGGATCCT GTCATATTAA ACATGTGCCA GTCACATCAT 
GTTATTTTCT GGGATTTAAT CGATGTAGCC ACTACCTTTG CTAGAGTTGA TTCTTCATTT 
TATACTTCTG AAAACCTAAG ACGAGAACTG GATAAGGCTT TTGATAGGTC CGGTACTGCT 
GCCAAATTAA AACATCCAAA AAAAACCGTA GTAGAAACTT TAGATATTCT CACTACGTTT 
AATTTACCTG AAATACGTCT CAATGTGCAC ACCGATGATT TCTGGATTCA CGGTGGGGAT 
TTAACACAAT TGCATTCCAT ATTAAGCTTC TTTGGATTCT CACTTGATTA TAATTTTTAT 
TCAAGCGGTC GTTGTTATGC TGAGTTTTCA ATCGATTCTA TACAATTAAA AGATTGCAAT 
CCTCAAGATA ATGTCGTTTT TTTGGATGTA TTAGAATACA GTGAAAATCA TAACAGACTT 
GTCAATGGTT GCCTTGAATA TGATTCTCAA AATCCAAGAT ACAACCTCGT ACTAGATATT 
GATTCACCGA AGATATTTGT CAATTTGAAT TATTTGTATT CTATTTGGTC TATATTTGTT 
CATTGGCATC GTGCTTACTA TTCGCACTTG GATTATTTAA CAGAGGTTGA ATACTTTATT 
ATGGGAAATC CAAACCAAAA TGCTTGTGGA GAAGAAAGCT ATTGGTACTA CAGAATTACA 
TTTGTTGATA TGACTTTGCT CTTTTTTAGA AACGTATCTG ATGCCAATCT TTACTCACTA 
CCAATGTTTT TTGGAGAGCT ATTAATTACC CAACAATCGA TCTTTGCGGT TACTGCTAAC 
AACATGAAGA TTAATGCTTG TCCTCTCAGC GAGACGGCCA ATATATCTAA TCAGTTAGCA 
GACCCTTTTG GATTTCGTTA CACATATTCG CAGCATACCG TCAACAAAAT TCAGATAATC 
ACCAACATCA CTCTTGATTT TGATTCGTTT GTCTTAAGGA CTACAGTGAA CGACTTTCTG 
TTCCTCCAAA CTATTTTACG TAAGATATAT AATTTTTATT ATGCCCTATA CGATGTCCCC 
ACAACCGATG TTGAATTGCT GAAGAGAACG AAGGATGATC AACTAGCGAC GAATCCCGAT 
TTTCTTCAAT TGTCAGTAGA CACCGGTCAG CCTTCTTCTG TTTTTGGAAT ACGTATATGC 
AAGGAAGAAT TTTTATTAAC AGTTGACGGT ATTCGACTAT TGGTTATTAG TCAACTACAT 
GATCTTCCAC TTTTAAATAT TAACATTAAG CCTTTTCAGG TAGATCTTAA CGATTGGTCT 
TCCGAGTTAA ATTCAAATGC ACATTTGGAG CTTTTTATGA ATTTTTATAA CTTCTCTAAC 
TCTCATTGGG AGCCCTTTTT AGAGCCCTGG AAAGTAGGTG TACATATTTC TAGGAATCCA 
AACACATCGA AGACTGCGGT ACATGTTTTT TCGAGAGAGA AATTAGATCT GGTTATTACT 
CCTCAGTTAA TTGAGACTCT ACATTTTGGT TTCACCAAAG TTATCAGTAC TCCATTTCCT 
ATTGAATTCA AATGTGACGC ACCTTATCGT ATTCACAACT ACACAGGACA TGCTGTTAGT 
GTTTGGGCAG ATTTTGAAAA TGCTGCAGAT TCTTGCGTTA GACATCTCGA AAATAACGAA 
GAAACCGACT GGAAATTTGA AGAATGGAGA CAAATGCAGG ATGTGGTTAA ACAAGATCAG 
GATAGATCTT ATATAGGGTT CCATTTCGAA AACAGTAAAT GGGAGTCTTT ACGACATGTA 
CGAGTTAATC GTGTAGGAGA GCATATATAT CCCTTAATTT CCTATGATCA AGACGAGCTA 
AAGCATTATA TGGTAGTTGA TGTCAATTTG GGAGAAGATT ATATTAAACA TATCACTCTA 
CGTTCCCCAC TTTTATTGAT AAATGAGACC CAAATGGAAA TAGATGTAGT TTTTTGCGAC 
TCAGATGGCA TTCAAAGGTC ACAGATATAC CACATGAGCC CTGAAGAGAG TTGCTCTTTG 
CCAATTGAAA CTGCTTATTA TTATTCAATT CACATTAGAC CGGTTTCAGA ATTTAAATTT 
AATTGGACTT CGGAAGCTAT ATCTTGGAAA GATCTAGTTG ACAACAAACA GAGCCTTGTG 
ACATGCCAAC ATTCAGATAA CACCTTCTCG ACTCCTGCGT GTCGTTTTGC CGCTAATGCA 
GAACTTAAAA GTCAAACTAT ATCCAACCAT TATCCTTTTA TGCATATTAC GATTTCTGCG 
CTCCTAGAAG TGAAAAACTT ATTGCCAATT GATCTTAACA TTCGAATCAT CGATAAGGAC 
CAAGAGGGAG TATGGATGTC GAATGTTGGT ATTGGAGAGT GCGCATATGT TCACTCGATA 
AATATTTCCC ACGTTTTGCT CCTACAAGCC GAGTCATCAG AGTCGCATTA CCTTCCCAGC 
TCCTTGGCTA CTATTATCAC TAACGACTCT GCTCAAGAAA GAGATGAGTA CATGACAATC 
ACTCTTCAGG GAGGTAGAAA AACAAGATTA GGGTTGAGTT ATACTGAAAA GTATCCTGGA 
ATTTATCACA TCGAAATTTT TAGCCCGTAC ATAATAATTA ACAAGTCAGG GTCCTTTCTG 
TTTGTTGGTC CAAAAAATGA TTACAACCGT ATTTCTTTTT CTTCAGCTTC TCTTTCGTCT 
GGGGAAGACG GCAAAGTTGT TCCATGTATG TTTTCATATT CGCATAACTA TGGCAGCAGA 
AGATGTCGGT TACGAGCAGA TAATTCTAAT TGGAGTGAGC CCGTTAGCTT TGATGCGATT 
GGCAGTGTGT TTGAAGTAGA ATTACCTTCC AAGGAAGACC ATAATAAAGT TTACAGATTA 
GGTATTTTTG TTGAAACGGG ACCCGATGGT TACTCGAAAA CTAATATCGT TACAATAACT 
TCTAGATTCA TAGTGAGAAA TAAAACAAGA TGGAGTTTGG TGATTGCGGA GCCTTACAAC 
GATTTTATTG CGGAAATCGC TCCTGAGGGT GAAGAGTTTT TGACATATTT AAGAAAGCAT 
TCACACCCGA TGTTAAAGCT ATCCTCCTCA GATTGCTACC TTTGGTCATC ATCATTTTAT 
ATTGAGGAGA TAGGGTCAAC TCATGTACGT TTAATGACCT CGGAAGGGGA AAAACTTTTA 
CGCTTGGAAA TTGTCATAAA AAATGCCACA ATCTTTATCA GTATATTTGA AGAGACTGGG 
GACTGGCCAT ACTATATAAA AAATGAATCT GGTGTTTTGT TAAAATTTTG GCAGGTAAAT 
CCTATTGATG CCTCAGAAGG AAAGAATAAT ACTGCTTTGT TGAAGTACCA TGATATACCG 
CCTCATTCAG AGGTAAAGTA TTCTTGGGAT TATCCCTGTT GTGCTAATAA GGAAATTGCT 
TTATGCTATG GTGATCAAAA ATGCTTAACT ACTTTGGCAG AAATTGGCCC CCTTTCTCCC 
TTTAAATTCA CCGATGCTTC CAATAATACA AAATTTATTT CACGAGATAT AGTCGCTAAT 
GGGCTTTCGA AAATTCTTAT TCTAAAAGAC TATGATCCTT CAAAGGCTGT GAGGAAGCCG 
AAGATATACA GCAAAGTCTC AACTGAAGAA CGCGATTTTA ATTTGGAGCA ATTTGACTCC 
GGGATTGACT TGTCCGTCAA ATTTTTGCTG GAGGGAATTG GGATATCTTT GGTAGAACGG 
AATACGCAAG AATTAGCGTA TTTGACCTTT CATGGGATCA ACTTGTTCTT TACAGATTCC 
CATCTTATAC GCACTTTTAA ATTAGATGTT CGATGGATAC AAATTGACAA CCAGCTTTAT 
GGAGGAATTT ATCCAATTAT CCTCTATCCA AGTATTCTAT CACAGGAAGA CACAATGAAT 
GACAATTCAC TTTTACCAAC GTTTCACTCA ATGGTTGCAG TTGTCAAGAA TGATACATAC 
GGTGTGACTT ACGTAAAATA TGCTACTATA TTACTTCAGG AATTAACTAT TGAAATTGAT 
GAGGACTTTG CTTTTGCTGC ACTCGAATAC ATCAAGGACT CTGTGCCCCG AAGCAAAAGA 
AATACCGGCA AGATGTTTGA TGATTCGCTG GAACTTGTAC CAGAAAATCT AGGAAATGAT 
TTGAAAGTGT ATTTTGAAGT GCTTAATTTG CAACCCACTG AAATGCATCT GTCTTTCGTT 
CGTACGGAGA GGATCAATAA TACTGATGGC ACCGTTGTCT CTTCACATAA TCCATTTGTC 
TTTTTCGTGA ATGTTTTATC AATGGCAATA GGAAACATAA ATGATGCACC AGTCCGTTTA 
AATGCTCTTC TCATGGATAA TGCACACGTT TCTCTCCGCC GTCTTTTTGA ACTTGTCAAA 
AACCATTATT CTCAAGAACT TTTATCCCAA GTGCATAAGA TTGTTGGATC AGCAGACTTT 
TTGGGAAATC CTGTTGGCTT ATTTACCACA ATAACGTCCG GTTTTGCTGA TATATTTTAT 
GAACCCTTTC ATGGTTTTAT CTTAAACGAG GGTTCTTATG AATTAGGTAT AGGATTTGCC 
AAGGGTACTG CAAGCTTTAT TAAAAAGGCA GTATTCGGAA TCACTGACAG TATTTCAAAA 
GTAACGGGCA CTATTTCAAG AAGCTTAAGT GTGATCACAT TGGATCCCAA ATTCCAAAGC 
CGAAGACGTG CTGCTCGTAT CAGAAATCGG CCTGTTCATA TACTTTATGG AGTGACAGCT 
GGAGCTGCGT CACTTTACAC AGGAGTTCGA AGTGGTGTCA GAGGATTAGC CTTACAACCT 
ATTATTGGAG CAAGGAGAAA TGGACTTCCT GGCCTTGTTA AAGGCTTAGG TAAGGGTTTG 
GTGGGTTTCA CGACAAAACC ATTGGTAGGG TTATTCGATT TTGCGTCAAG TATCTCTGAA 
GGCGCAAGAA ATACAACAAC TGTGTTTGAT GAACGACACA TAGAAAAGCT AAGATTGTCA 
AGACTGATGA GTGATGATGG TGTCGTATAT CCTTTTCAAC TACGGGAGGC TTTGGGACAG 
TACTGGCTAA AACATCTCGA TAATGGAAGA TATTTTAAAG ATTTTTATAA GGCTCATATC 
ATTATTGAAA ATAAAGTGCT TGTGATCTTA ACGAATAATA GGATATTGTT TGTTCAGCCT 
CAACAGCTTA ATTGCAAAAA AGAAATTCAT CTCTCCAAAG TTAAAACTGT TAAGTTACAG 
AGCAAAGAGC ATATCTTTCT CCAACTTTTG AAGGGTGTAA ACTTTGAATT TTCTGTCCCC 
GAAAACTCCG TTCGGACATT TTTCTACCGA AAAATTCGAG ACGAGCTTGC CGCTTATAAA 
CACAAAGTAA ATTACGAGCT AGAGGTTGCT CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342827.1
CDS	73..9288
Translation	MLEGLVAGLLNKILGSYVDNLDTKQLNIGVWGGHVSLHNLRIKPEALDKLGIPIEITSGLIGTFTLEIPWSNLRNKSLTINIEDIYLSIHPQAKNSLTRDELEQSQQALKQEQLDSFEILRKNFRETLEESSSNPNISRKQSFIEYLIAKLTDNIQIYIERIHLRFEDNLSDLEKPYSLGLTLYSLRVTSTDASFTEYLLSTDPIPSSCIHKIITVDYFSIYWISKCEISKCTTTEDIFSYLKNLIPSAEKSPAYNYILKPLRATAHVVLFRHPTDQIMQLRGKLSVEEISITLSDHMYYSLLGVIDYFRVVMKQQYYLQYRPKSTPKEKPLEWFKYAILVVKDSVHESRYHWTWKYFKRRRDDRIAYMHIIRKRYLNEQISKEEIDLQKKIEKRNSTYDLIKYRSRVHTSLIEERNSIYLKPKTSAAHGLYDWFSGYIRKPQSQDEDTLASTDKTAADLTDQEQKEFFSAIEWSGQLYPDTVNLDPDMCMANVEVSIAKGSFVIQSHINGRVIPLIKQRFESFATECFIRPQSLKLKVSLKDLDMFDGITNPELEPARVIFAKPSVEESESLQKIPEAYRTHLFFLLLDTKPVYKASSTLIVHLRTLVIIYNRVCIESLLAFFVPPRTKIEHVSEWGYSAAAKVMTLARQTRASLDYALEMHKTSDMTIDLQAPLIVVREECTDLKSPTLFLDVGRALVHTQLVDDAIIDKFRKLQSKKINNEQLKQLENLMYDKFTISLFNVRCLIGPDYETGWRCLPKGCDYHILKECSLDINFEISILQKATNLTKFKVSSHMKHAEIMFSDVQYKVFINMMSNILPTLPVAEIPFTYQQFLDAVKPPPFFDAPDNFQITHTSLGSHANENTAAQFMAQQIFAFYFKVDYAICSLYRRSENYLIPVVRAFTEFYIDLVVRKFDYLVTSKLNDLVIKEFTYPSSLCDNVLVRSSPSPKNNFDDTVFISYTSIDYDSPELDSVYEGVRTTIAVVLSDLILNVEPTGFSFVYDFIRATFTSLNDEYMIGEDPELTRKISPVEGIIPEDANVRFDNVDIFLYDCDQHFSTVCLYSANMHMEFREKFFLQARFYDLEVKNHMKSNNPPKTIVKIDDNDLFIFKYESYDIPKDISKPTCDCVYDISFGSLTFYFQKSYFNAIYDFLLKLKRFQELFSSIRYAIYYKLYGNKVSLTYPKFELRIKHPKVYFDDVLDEERNCRMQLIVKPQSFYAFSKCPIVEKNSKKSIFSCEITKVEFHTAVPSSSHHDVLMEENNVHLDLTYDANYTTGAYVFKATGDLDPVILNMCQSHHVIFWDLIDVATTFARVDSSFYTSENLRRELDKAFDRSGTAAKLKHPKKTVVETLDILTTFNLPEIRLNVHTDDFWIHGGDLTQLHSILSFFGFSLDYNFYSSGRCYAEFSIDSIQLKDCNPQDNVVFLDVLEYSENHNRLVNGCLEYDSQNPRYNLVLDIDSPKIFVNLNYLYSIWSIFVHWHRAYYSHLDYLTEVEYFIMGNPNQNACGEESYWYYRITFVDMTLLFFRNVSDANLYSLPMFFGELLITQQSIFAVTANNMKINACPLSETANISNQLADPFGFRYTYSQHTVNKIQIITNITLDFDSFVLRTTVNDFLFLQTILRKIYNFYYALYDVPTTDVELLKRTKDDQLATNPDFLQLSVDTGQPSSVFGIRICKEEFLLTVDGIRLLVISQLHDLPLLNINIKPFQVDLNDWSSELNSNAHLELFMNFYNFSNSHWEPFLEPWKVGVHISRNPNTSKTAVHVFSREKLDLVITPQLIETLHFGFTKVISTPFPIEFKCDAPYRIHNYTGHAVSVWADFENAADSCVRHLENNEETDWKFEEWRQMQDVVKQDQDRSYIGFHFENSKWESLRHVRVNRVGEHIYPLISYDQDELKHYMVVDVNLGEDYIKHITLRSPLLLINETQMEIDVVFCDSDGIQRSQIYHMSPEESCSLPIETAYYYSIHIRPVSEFKFNWTSEAISWKDLVDNKQSLVTCQHSDNTFSTPACRFAANAELKSQTISNHYPFMHITISALLEVKNLLPIDLNIRIIDKDQEGVWMSNVGIGECAYVHSINISHVLLLQAESSESHYLPSSLATIITNDSAQERDEYMTITLQGGRKTRLGLSYTEKYPGIYHIEIFSPYIIINKSGSFLFVGPKNDYNRISFSSASLSSGEDGKVVPCMFSYSHNYGSRRCRLRADNSNWSEPVSFDAIGSVFEVELPSKEDHNKVYRLGIFVETGPDGYSKTNIVTITSRFIVRNKTRWSLVIAEPYNDFIAEIAPEGEEFLTYLRKHSHPMLKLSSSDCYLWSSSFYIEEIGSTHVRLMTSEGEKLLRLEIVIKNATIFISIFEETGDWPYYIKNESGVLLKFWQVNPIDASEGKNNTALLKYHDIPPHSEVKYSWDYPCCANKEIALCYGDQKCLTTLAEIGPLSPFKFTDASNNTKFISRDIVANGLSKILILKDYDPSKAVRKPKIYSKVSTEERDFNLEQFDSGIDLSVKFLLEGIGISLVERNTQELAYLTFHGINLFFTDSHLIRTFKLDVRWIQIDNQLYGGIYPIILYPSILSQEDTMNDNSLLPTFHSMVAVVKNDTYGVTYVKYATILLQELTIEIDEDFAFAALEYIKDSVPRSKRNTGKMFDDSLELVPENLGNDLKVYFEVLNLQPTEMHLSFVRTERINNTDGTVVSSHNPFVFFVNVLSMAIGNINDAPVRLNALLMDNAHVSLRRLFELVKNHYSQELLSQVHKIVGSADFLGNPVGLFTTITSGFADIFYEPFHGFILNEGSYELGIGFAKGTASFIKKAVFGITDSISKVTGTISRSLSVITLDPKFQSRRRAARIRNRPVHILYGVTAGAASLYTGVRSGVRGLALQPIIGARRNGLPGLVKGLGKGLVGFTTKPLVGLFDFASSISEGARNTTTVFDERHIEKLRLSRLMSDDGVVYPFQLREALGQYWLKHLDNGRYFKDFYKAHIIIENKVLVILTNNRILFVQPQQLNCKKEIHLSKVKTVKLQSKEHIFLQLLKGVNFEFSVPENSVRTFFYRKIRDELAAYKHKVNYELEVAL

Target ORF information:

RefSeq Version	NM_001355826.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe vacuolar protein sorting-associated protein (vps13a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355826.1

ORF Insert Sequence:

ATGTTAGAAG GCCTAGTTGC CGGCCTCTTG AACAAAATTT TAGGTTCGTA TGTTGACAAC 
TTGGATACTA AACAACTAAA CATCGGCGTT TGGGGTGGCC ATGTTAGTTT GCATAATTTA 
CGAATAAAAC CTGAGGCCTT AGATAAACTC GGAATTCCCA TTGAAATCAC TAGTGGTTTA 
ATTGGCACGT TTACGCTGGA AATTCCATGG TCGAATCTTC GGAACAAATC TTTAACGATC 
AATATTGAAG ACATATATTT GTCAATTCAT CCCCAAGCGA AAAACTCTTT AACACGCGAT 
GAGTTGGAGC AATCTCAACA AGCTTTAAAG CAGGAACAGC TGGATAGTTT TGAAATCTTA 
CGGAAAAATT TTCGAGAAAC TTTAGAAGAG TCTTCATCAA ATCCAAATAT AAGTAGAAAA 
CAATCTTTTA TTGAGTATCT TATTGCCAAA CTTACTGATA ACATTCAAAT ATACATCGAA 
AGAATTCACT TGCGGTTTGA AGATAATTTG AGTGATCTTG AAAAACCTTA TTCTCTTGGA 
TTGACGCTTT ATTCGTTAAG AGTAACCTCT ACAGATGCTA GTTTTACCGA ATATTTGCTT 
TCAACCGATC CAATTCCTTC CTCTTGCATT CATAAAATTA TTACTGTTGA TTATTTCTCA 
ATATACTGGA TAAGTAAGTG TGAAATTTCT AAATGCACTA CTACAGAAGA CATATTCTCT 
TATTTAAAGA ACCTGATTCC TAGTGCAGAA AAGTCACCTG CTTATAATTA CATTTTAAAG 
CCGCTACGGG CAACCGCCCA TGTTGTCTTA TTTCGACATC CGACAGACCA GATAATGCAA 
TTAAGGGGAA AATTATCTGT TGAAGAAATA TCAATTACGC TAAGTGATCA TATGTATTAC 
AGTTTACTTG GTGTTATTGA CTACTTTCGT GTTGTAATGA AGCAACAATA TTATCTTCAA 
TATAGGCCTA AATCGACTCC TAAAGAGAAA CCATTGGAGT GGTTTAAGTA TGCCATTTTA 
GTTGTTAAAG ATTCTGTTCA TGAGAGCAGA TATCACTGGA CTTGGAAATA CTTTAAAAGG 
AGAAGGGACG ACAGAATAGC TTACATGCAT ATTATTCGTA AGAGGTATTT GAATGAGCAA 
ATATCAAAGG AAGAAATTGA TTTGCAAAAA AAGATAGAGA AACGAAATTC TACTTACGAT 
CTAATTAAGT ACCGTTCACG TGTGCATACA TCTCTGATTG AGGAAAGGAA TTCTATTTAT 
TTAAAACCAA AAACTTCGGC AGCTCATGGA TTGTACGATT GGTTTTCTGG GTATATACGA 
AAGCCGCAGT CCCAGGATGA AGATACATTA GCAAGTACTG ATAAAACAGC CGCTGACTTG 
ACTGATCAAG AACAGAAAGA ATTTTTTTCT GCAATAGAAT GGAGCGGACA ATTATATCCT 
GATACCGTAA ATTTAGATCC GGATATGTGT ATGGCTAATG TTGAAGTCTC TATCGCTAAA 
GGAAGTTTTG TCATACAATC TCACATTAAT GGACGAGTAA TACCTTTAAT TAAGCAAAGG 
TTTGAGAGTT TTGCAACAGA ATGTTTTATA AGGCCTCAAT CACTGAAATT GAAAGTGTCT 
CTTAAGGATT TGGATATGTT TGATGGCATA ACGAACCCCG AGCTTGAGCC TGCTCGTGTA 
ATTTTTGCAA AGCCTTCTGT TGAAGAAAGT GAAAGTTTGC AAAAAATCCC TGAAGCCTAC 
AGAACACATT TGTTTTTTTT ATTGTTAGAC ACCAAACCGG TGTATAAAGC GAGTTCTACT 
CTAATCGTAC ATTTGCGAAC TTTGGTTATT ATTTACAATC GAGTTTGTAT TGAAAGTCTG 
CTAGCTTTTT TTGTTCCTCC AAGAACAAAA ATCGAGCACG TTTCTGAATG GGGATATTCT 
GCAGCTGCAA AAGTTATGAC CCTAGCTAGA CAAACTCGTG CTAGTTTGGA TTATGCCCTA 
GAAATGCACA AGACTTCCGA TATGACAATT GACCTGCAAG CTCCCTTGAT TGTTGTTCGA 
GAAGAATGTA CGGATTTAAA AAGCCCTACA CTGTTCCTTG ACGTGGGTAG AGCGTTGGTA 
CATACCCAAT TAGTTGATGA TGCGATTATC GACAAATTCA GAAAACTCCA GAGCAAAAAG 
ATAAACAACG AGCAATTAAA ACAACTAGAA AACTTGATGT ACGACAAGTT TACCATCTCA 
TTATTTAATG TTCGATGCTT AATTGGGCCT GATTATGAAA CTGGTTGGCG TTGCTTACCC 
AAAGGGTGTG ATTACCATAT TTTGAAAGAA TGCTCTCTTG ACATTAACTT TGAAATATCA 
ATCCTTCAAA AAGCTACTAA CTTAACGAAG TTCAAAGTTT CAAGTCATAT GAAGCATGCC 
GAAATTATGT TTTCTGATGT GCAATATAAG GTATTCATTA ATATGATGAG CAATATATTG 
CCTACGCTTC CTGTTGCTGA AATACCATTT ACATACCAAC AATTCCTTGA CGCGGTTAAG 
CCTCCTCCTT TCTTTGATGC TCCTGATAAT TTTCAAATAA CTCATACTTC TCTTGGAAGT 
CATGCTAATG AAAACACCGC TGCGCAATTT ATGGCCCAGC AAATTTTTGC TTTCTATTTT 
AAGGTGGATT ATGCTATATG TTCGTTGTAC CGAAGATCTG AAAACTATCT CATACCTGTG 
GTAAGAGCAT TTACAGAGTT TTACATCGAC CTAGTAGTGC GAAAATTTGA TTACCTTGTT 
ACTTCCAAGC TTAACGACTT GGTTATTAAA GAATTTACAT ATCCTTCTTC TCTTTGTGAC 
AATGTTTTAG TTCGTTCTTC TCCTTCACCT AAAAATAACT TTGATGATAC CGTTTTCATC 
AGCTATACAA GCATTGATTA TGACTCTCCT GAACTAGATT CTGTTTACGA AGGTGTTCGA 
ACTACCATTG CTGTAGTCCT TTCTGATCTC ATTCTCAACG TTGAGCCTAC TGGATTTTCA 
TTTGTCTACG ATTTTATCAG AGCCACTTTT ACCTCTTTAA ATGATGAGTA TATGATTGGT 
GAAGATCCAG AGTTGACTCG AAAAATTTCA CCAGTTGAAG GAATTATTCC AGAAGACGCC 
AACGTTCGGT TTGACAACGT AGATATTTTT CTTTATGACT GTGATCAACA TTTTTCAACA 
GTTTGTCTGT ATAGTGCTAA CATGCATATG GAATTCAGAG AGAAGTTTTT CCTTCAAGCA 
CGATTTTATG ACCTTGAAGT TAAAAACCAT ATGAAAAGTA ATAATCCGCC AAAGACCATT 
GTGAAGATTG ACGATAATGA TTTATTTATA TTTAAATATG AGAGTTATGA CATTCCAAAA 
GACATATCGA AACCTACATG CGACTGTGTT TATGATATAT CTTTTGGCTC ATTAACTTTT 
TATTTTCAAA AATCATATTT CAATGCAATA TACGATTTTT TATTAAAGTT GAAGCGATTT 
CAGGAATTAT TTAGCTCAAT TAGATATGCG ATCTACTATA AGCTTTACGG AAACAAAGTT 
AGCCTAACTT ATCCTAAATT TGAGCTTCGA ATTAAGCATC CAAAGGTATA CTTTGATGAT 
GTATTAGATG AAGAAAGAAA TTGTCGGATG CAATTGATAG TGAAACCCCA ATCCTTTTAT 
GCGTTTAGTA AATGTCCGAT TGTGGAAAAG AACTCGAAGA AATCAATATT CAGTTGTGAA 
ATAACTAAGG TTGAATTTCA TACTGCCGTC CCTAGTTCCT CGCATCACGA TGTACTAATG 
GAGGAAAATA ATGTTCATTT GGACCTTACA TATGACGCTA ATTACACTAC TGGTGCTTAT 
GTATTCAAAG CAACTGGTGA TTTGGATCCT GTCATATTAA ACATGTGCCA GTCACATCAT 
GTTATTTTCT GGGATTTAAT CGATGTAGCC ACTACCTTTG CTAGAGTTGA TTCTTCATTT 
TATACTTCTG AAAACCTAAG ACGAGAACTG GATAAGGCTT TTGATAGGTC CGGTACTGCT 
GCCAAATTAA AACATCCAAA AAAAACCGTA GTAGAAACTT TAGATATTCT CACTACGTTT 
AATTTACCTG AAATACGTCT CAATGTGCAC ACCGATGATT TCTGGATTCA CGGTGGGGAT 
TTAACACAAT TGCATTCCAT ATTAAGCTTC TTTGGATTCT CACTTGATTA TAATTTTTAT 
TCAAGCGGTC GTTGTTATGC TGAGTTTTCA ATCGATTCTA TACAATTAAA AGATTGCAAT 
CCTCAAGATA ATGTCGTTTT TTTGGATGTA TTAGAATACA GTGAAAATCA TAACAGACTT 
GTCAATGGTT GCCTTGAATA TGATTCTCAA AATCCAAGAT ACAACCTCGT ACTAGATATT 
GATTCACCGA AGATATTTGT CAATTTGAAT TATTTGTATT CTATTTGGTC TATATTTGTT 
CATTGGCATC GTGCTTACTA TTCGCACTTG GATTATTTAA CAGAGGTTGA ATACTTTATT 
ATGGGAAATC CAAACCAAAA TGCTTGTGGA GAAGAAAGCT ATTGGTACTA CAGAATTACA 
TTTGTTGATA TGACTTTGCT CTTTTTTAGA AACGTATCTG ATGCCAATCT TTACTCACTA 
CCAATGTTTT TTGGAGAGCT ATTAATTACC CAACAATCGA TCTTTGCGGT TACTGCTAAC 
AACATGAAGA TTAATGCTTG TCCTCTCAGC GAGACGGCCA ATATATCTAA TCAGTTAGCA 
GACCCTTTTG GATTTCGTTA CACATATTCG CAGCATACCG TCAACAAAAT TCAGATAATC 
ACCAACATCA CTCTTGATTT TGATTCGTTT GTCTTAAGGA CTACAGTGAA CGACTTTCTG 
TTCCTCCAAA CTATTTTACG TAAGATATAT AATTTTTATT ATGCCCTATA CGATGTCCCC 
ACAACCGATG TTGAATTGCT GAAGAGAACG AAGGATGATC AACTAGCGAC GAATCCCGAT 
TTTCTTCAAT TGTCAGTAGA CACCGGTCAG CCTTCTTCTG TTTTTGGAAT ACGTATATGC 
AAGGAAGAAT TTTTATTAAC AGTTGACGGT ATTCGACTAT TGGTTATTAG TCAACTACAT 
GATCTTCCAC TTTTAAATAT TAACATTAAG CCTTTTCAGG TAGATCTTAA CGATTGGTCT 
TCCGAGTTAA ATTCAAATGC ACATTTGGAG CTTTTTATGA ATTTTTATAA CTTCTCTAAC 
TCTCATTGGG AGCCCTTTTT AGAGCCCTGG AAAGTAGGTG TACATATTTC TAGGAATCCA 
AACACATCGA AGACTGCGGT ACATGTTTTT TCGAGAGAGA AATTAGATCT GGTTATTACT 
CCTCAGTTAA TTGAGACTCT ACATTTTGGT TTCACCAAAG TTATCAGTAC TCCATTTCCT 
ATTGAATTCA AATGTGACGC ACCTTATCGT ATTCACAACT ACACAGGACA TGCTGTTAGT 
GTTTGGGCAG ATTTTGAAAA TGCTGCAGAT TCTTGCGTTA GACATCTCGA AAATAACGAA 
GAAACCGACT GGAAATTTGA AGAATGGAGA CAAATGCAGG ATGTGGTTAA ACAAGATCAG 
GATAGATCTT ATATAGGGTT CCATTTCGAA AACAGTAAAT GGGAGTCTTT ACGACATGTA 
CGAGTTAATC GTGTAGGAGA GCATATATAT CCCTTAATTT CCTATGATCA AGACGAGCTA 
AAGCATTATA TGGTAGTTGA TGTCAATTTG GGAGAAGATT ATATTAAACA TATCACTCTA 
CGTTCCCCAC TTTTATTGAT AAATGAGACC CAAATGGAAA TAGATGTAGT TTTTTGCGAC 
TCAGATGGCA TTCAAAGGTC ACAGATATAC CACATGAGCC CTGAAGAGAG TTGCTCTTTG 
CCAATTGAAA CTGCTTATTA TTATTCAATT CACATTAGAC CGGTTTCAGA ATTTAAATTT 
AATTGGACTT CGGAAGCTAT ATCTTGGAAA GATCTAGTTG ACAACAAACA GAGCCTTGTG 
ACATGCCAAC ATTCAGATAA CACCTTCTCG ACTCCTGCGT GTCGTTTTGC CGCTAATGCA 
GAACTTAAAA GTCAAACTAT ATCCAACCAT TATCCTTTTA TGCATATTAC GATTTCTGCG 
CTCCTAGAAG TGAAAAACTT ATTGCCAATT GATCTTAACA TTCGAATCAT CGATAAGGAC 
CAAGAGGGAG TATGGATGTC GAATGTTGGT ATTGGAGAGT GCGCATATGT TCACTCGATA 
AATATTTCCC ACGTTTTGCT CCTACAAGCC GAGTCATCAG AGTCGCATTA CCTTCCCAGC 
TCCTTGGCTA CTATTATCAC TAACGACTCT GCTCAAGAAA GAGATGAGTA CATGACAATC 
ACTCTTCAGG GAGGTAGAAA AACAAGATTA GGGTTGAGTT ATACTGAAAA GTATCCTGGA 
ATTTATCACA TCGAAATTTT TAGCCCGTAC ATAATAATTA ACAAGTCAGG GTCCTTTCTG 
TTTGTTGGTC CAAAAAATGA TTACAACCGT ATTTCTTTTT CTTCAGCTTC TCTTTCGTCT 
GGGGAAGACG GCAAAGTTGT TCCATGTATG TTTTCATATT CGCATAACTA TGGCAGCAGA 
AGATGTCGGT TACGAGCAGA TAATTCTAAT TGGAGTGAGC CCGTTAGCTT TGATGCGATT 
GGCAGTGTGT TTGAAGTAGA ATTACCTTCC AAGGAAGACC ATAATAAAGT TTACAGATTA 
GGTATTTTTG TTGAAACGGG ACCCGATGGT TACTCGAAAA CTAATATCGT TACAATAACT 
TCTAGATTCA TAGTGAGAAA TAAAACAAGA TGGAGTTTGG TGATTGCGGA GCCTTACAAC 
GATTTTATTG CGGAAATCGC TCCTGAGGGT GAAGAGTTTT TGACATATTT AAGAAAGCAT 
TCACACCCGA TGTTAAAGCT ATCCTCCTCA GATTGCTACC TTTGGTCATC ATCATTTTAT 
ATTGAGGAGA TAGGGTCAAC TCATGTACGT TTAATGACCT CGGAAGGGGA AAAACTTTTA 
CGCTTGGAAA TTGTCATAAA AAATGCCACA ATCTTTATCA GTATATTTGA AGAGACTGGG 
GACTGGCCAT ACTATATAAA AAATGAATCT GGTGTTTTGT TAAAATTTTG GCAGGTAAAT 
CCTATTGATG CCTCAGAAGG AAAGAATAAT ACTGCTTTGT TGAAGTACCA TGATATACCG 
CCTCATTCAG AGGTAAAGTA TTCTTGGGAT TATCCCTGTT GTGCTAATAA GGAAATTGCT 
TTATGCTATG GTGATCAAAA ATGCTTAACT ACTTTGGCAG AAATTGGCCC CCTTTCTCCC 
TTTAAATTCA CCGATGCTTC CAATAATACA AAATTTATTT CACGAGATAT AGTCGCTAAT 
GGGCTTTCGA AAATTCTTAT TCTAAAAGAC TATGATCCTT CAAAGGCTGT GAGGAAGCCG 
AAGATATACA GCAAAGTCTC AACTGAAGAA CGCGATTTTA ATTTGGAGCA ATTTGACTCC 
GGGATTGACT TGTCCGTCAA ATTTTTGCTG GAGGGAATTG GGATATCTTT GGTAGAACGG 
AATACGCAAG AATTAGCGTA TTTGACCTTT CATGGGATCA ACTTGTTCTT TACAGATTCC 
CATCTTATAC GCACTTTTAA ATTAGATGTT CGATGGATAC AAATTGACAA CCAGCTTTAT 
GGAGGAATTT ATCCAATTAT CCTCTATCCA AGTATTCTAT CACAGGAAGA CACAATGAAT 
GACAATTCAC TTTTACCAAC GTTTCACTCA ATGGTTGCAG TTGTCAAGAA TGATACATAC 
GGTGTGACTT ACGTAAAATA TGCTACTATA TTACTTCAGG AATTAACTAT TGAAATTGAT 
GAGGACTTTG CTTTTGCTGC ACTCGAATAC ATCAAGGACT CTGTGCCCCG AAGCAAAAGA 
AATACCGGCA AGATGTTTGA TGATTCGCTG GAACTTGTAC CAGAAAATCT AGGAAATGAT 
TTGAAAGTGT ATTTTGAAGT GCTTAATTTG CAACCCACTG AAATGCATCT GTCTTTCGTT 
CGTACGGAGA GGATCAATAA TACTGATGGC ACCGTTGTCT CTTCACATAA TCCATTTGTC 
TTTTTCGTGA ATGTTTTATC AATGGCAATA GGAAACATAA ATGATGCACC AGTCCGTTTA 
AATGCTCTTC TCATGGATAA TGCACACGTT TCTCTCCGCC GTCTTTTTGA ACTTGTCAAA 
AACCATTATT CTCAAGAACT TTTATCCCAA GTGCATAAGA TTGTTGGATC AGCAGACTTT 
TTGGGAAATC CTGTTGGCTT ATTTACCACA ATAACGTCCG GTTTTGCTGA TATATTTTAT 
GAACCCTTTC ATGGTTTTAT CTTAAACGAG GGTTCTTATG AATTAGGTAT AGGATTTGCC 
AAGGGTACTG CAAGCTTTAT TAAAAAGGCA GTATTCGGAA TCACTGACAG TATTTCAAAA 
GTAACGGGCA CTATTTCAAG AAGCTTAAGT GTGATCACAT TGGATCCCAA ATTCCAAAGC 
CGAAGACGTG CTGCTCGTAT CAGAAATCGG CCTGTTCATA TACTTTATGG AGTGACAGCT 
GGAGCTGCGT CACTTTACAC AGGAGTTCGA AGTGGTGTCA GAGGATTAGC CTTACAACCT 
ATTATTGGAG CAAGGAGAAA TGGACTTCCT GGCCTTGTTA AAGGCTTAGG TAAGGGTTTG 
GTGGGTTTCA CGACAAAACC ATTGGTAGGG TTATTCGATT TTGCGTCAAG TATCTCTGAA 
GGCGCAAGAA ATACAACAAC TGTGTTTGAT GAACGACACA TAGAAAAGCT AAGATTGTCA 
AGACTGATGA GTGATGATGG TGTCGTATAT CCTTTTCAAC TACGGGAGGC TTTGGGACAG 
TACTGGCTAA AACATCTCGA TAATGGAAGA TATTTTAAAG ATTTTTATAA GGCTCATATC 
ATTATTGAAA ATAAAGTGCT TGTGATCTTA ACGAATAATA GGATATTGTT TGTTCAGCCT 
CAACAGCTTA ATTGCAAAAA AGAAATTCAT CTCTCCAAAG TTAAAACTGT TAAGTTACAG 
AGCAAAGAGC ATATCTTTCT CCAACTTTTG AAGGGTGTAA ACTTTGAATT TTCTGTCCCC 
GAAAACTCCG TTCGGACATT TTTCTACCGA AAAATTCGAG ACGAGCTTGC CGCTTATAAA 
CACAAAGTAA ATTACGAGCT AGAGGTTGCT CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

vps13a ( XM_001713095.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> XP_001713147.1 Schizosaccharomyces pombe 972h- vacuolar protein sorting-associated protein (vps13a), mRNA.

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CloneID	OSj01810
Clone ID Related Accession (Same CDS sequence)	XM_001713095.2
Accession Version	XM_001713095.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	vacuolar protein sorting-associated protein
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced XM_001713095.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGTTAGAAG GCCTAGTTGC CGGCCTCTTG AACAAAATTT TAGGTTCGTA TGTTGACAAC 
TTGGATACTA AACAACTAAA CATCGGCGTT TGGGGTGGCC ATGTTAGTTT GCATAATTTA 
CGAATAAAAC CTGAGGCCTT AGATAAACTC GGAATTCCCA TTGAAATCAC TAGTGGTTTA 
ATTGGCACGT TTACGCTGGA AATTCCATGG TCGAATCTTC GGAACAAATC TTTAACGATC 
AATATTGAAG ACATATATTT GTCAATTCAT CCCCAAGCGA AAAACTCTTT AACACGCGAT 
GAGTTGGAGC AATCTCAACA AGCTTTAAAG CAGGAACAGC TGGATAGTTT TGAAATCTTA 
CGGAAAAATT TTCGAGAAAC TTTAGAAGAG TCTTCATCAA ATCCAAATAT AAGTAGAAAA 
CAATCTTTTA TTGAGTATCT TATTGCCAAA CTTACTGATA ACATTCAAAT ATACATCGAA 
AGAATTCACT TGCGGTTTGA AGATAATTTG AGTGATCTTG AAAAACCTTA TTCTCTTGGA 
TTGACGCTTT ATTCGTTAAG AGTAACCTCT ACAGATGCTA GTTTTACCGA ATATTTGCTT 
TCAACCGATC CAATTCCTTC CTCTTGCATT CATAAAATTA TTACTGTTGA TTATTTCTCA 
ATATACTGGA TAAGTAAGTG TGAAATTTCT AAATGCACTA CTACAGAAGA CATATTCTCT 
TATTTAAAGA ACCTGATTCC TAGTGCAGAA AAGTCACCTG CTTATAATTA CATTTTAAAG 
CCGCTACGGG CAACCGCCCA TGTTGTCTTA TTTCGACATC CGACAGACCA GATAATGCAA 
TTAAGGGGAA AATTATCTGT TGAAGAAATA TCAATTACGC TAAGTGATCA TATGTATTAC 
AGTTTACTTG GTGTTATTGA CTACTTTCGT GTTGTAATGA AGCAACAATA TTATCTTCAA 
TATAGGCCTA AATCGACTCC TAAAGAGAAA CCATTGGAGT GGTTTAAGTA TGCCATTTTA 
GTTGTTAAAG ATTCTGTTCA TGAGAGCAGA TATCACTGGA CTTGGAAATA CTTTAAAAGG 
AGAAGGGACG ACAGAATAGC TTACATGCAT ATTATTCGTA AGAGGTATTT GAATGAGCAA 
ATATCAAAGG AAGAAATTGA TTTGCAAAAA AAGATAGAGA AACGAAATTC TACTTACGAT 
CTAATTAAGT ACCGTTCACG TGTGCATACA TCTCTGATTG AGGAAAGGAA TTCTATTTAT 
TTAAAACCAA AAACTTCGGC AGCTCATGGA TTGTACGATT GGTTTTCTGG GTATATACGA 
AAGCCGCAGT CCCAGGATGA AGATACATTA GCAAGTACTG ATAAAACAGC CGCTGACTTG 
ACTGATCAAG AACAGAAAGA ATTTTTTTCT GCAATAGAAT GGAGCGGACA ATTATATCCT 
GATACCGTAA ATTTAGATCC GGATATGTGT ATGGCTAATG TTGAAGTCTC TATCGCTAAA 
GGAAGTTTTG TCATACAATC TCACATTAAT GGACGAGTAA TACCTTTAAT TAAGCAAAGG 
TTTGAGAGTT TTGCAACAGA ATGTTTTATA AGGCCTCAAT CACTGAAATT GAAAGTGTCT 
CTTAAGGATT TGGATATGTT TGATGGCATA ACGAACCCCG AGCTTGAGCC TGCTCGTGTA 
ATTTTTGCAA AGCCTTCTGT TGAAGAAAGT GAAAGTTTGC AAAAAATCCC TGAAGCCTAC 
AGAACACATT TGTTTTTTTT ATTGTTAGAC ACCAAACCGG TGTATAAAGC GAGTTCTACT 
CTAATCGTAC ATTTGCGAAC TTTGGTTATT ATTTACAATC GAGTTTGTAT TGAAAGTCTG 
CTAGCTTTTT TTGTTCCTCC AAGAACAAAA ATCGAGCACG TTTCTGAATG GGGATATTCT 
GCAGCTGCAA AAGTTATGAC CCTAGCTAGA CAAACTCGTG CTAGTTTGGA TTATGCCCTA 
GAAATGCACA AGACTTCCGA TATGACAATT GACCTGCAAG CTCCCTTGAT TGTTGTTCGA 
GAAGAATGTA CGGATTTAAA AAGCCCTACA CTGTTCCTTG ACGTGGGTAG AGCGTTGGTA 
CATACCCAAT TAGTTGATGA TGCGATTATC GACAAATTCA GAAAACTCCA GAGCAAAAAG 
ATAAACAACG AGCAATTAAA ACAACTAGAA AACTTGATGT ACGACAAGTT TACCATCTCA 
TTATTTAATG TTCGATGCTT AATTGGGCCT GATTATGAAA CTGGTTGGCG TTGCTTACCC 
AAAGGGTGTG ATTACCATAT TTTGAAAGAA TGCTCTCTTG ACATTAACTT TGAAATATCA 
ATCCTTCAAA AAGCTACTAA CTTAACGAAG TTCAAAGTTT CAAGTCATAT GAAGCATGCC 
GAAATTATGT TTTCTGATGT GCAATATAAG GTATTCATTA ATATGATGAG CAATATATTG 
CCTACGCTTC CTGTTGCTGA AATACCATTT ACATACCAAC AATTCCTTGA CGCGGTTAAG 
CCTCCTCCTT TCTTTGATGC TCCTGATAAT TTTCAAATAA CTCATACTTC TCTTGGAAGT 
CATGCTAATG AAAACACCGC TGCGCAATTT ATGGCCCAGC AAATTTTTGC TTTCTATTTT 
AAGGTGGATT ATGCTATATG TTCGTTGTAC CGAAGATCTG AAAACTATCT CATACCTGTG 
GTAAGAGCAT TTACAGAGTT TTACATCGAC CTAGTAGTGC GAAAATTTGA TTACCTTGTT 
ACTTCCAAGC TTAACGACTT GGTTATTAAA GAATTTACAT ATCCTTCTTC TCTTTGTGAC 
AATGTTTTAG TTCGTTCTTC TCCTTCACCT AAAAATAACT TTGATGATAC CGTTTTCATC 
AGCTATACAA GCATTGATTA TGACTCTCCT GAACTAGATT CTGTTTACGA AGGTGTTCGA 
ACTACCATTG CTGTAGTCCT TTCTGATCTC ATTCTCAACG TTGAGCCTAC TGGATTTTCA 
TTTGTCTACG ATTTTATCAG AGCCACTTTT ACCTCTTTAA ATGATGAGTA TATGATTGGT 
GAAGATCCAG AGTTGACTCG AAAAATTTCA CCAGTTGAAG GAATTATTCC AGAAGACGCC 
AACGTTCGGT TTGACAACGT AGATATTTTT CTTTATGACT GTGATCAACA TTTTTCAACA 
GTTTGTCTGT ATAGTGCTAA CATGCATATG GAATTCAGAG AGAAGTTTTT CCTTCAAGCA 
CGATTTTATG ACCTTGAAGT TAAAAACCAT ATGAAAAGTA ATAATCCGCC AAAGACCATT 
GTGAAGATTG ACGATAATGA TTTATTTATA TTTAAATATG AGAGTTATGA CATTCCAAAA 
GACATATCGA AACCTACATG CGACTGTGTT TATGATATAT CTTTTGGCTC ATTAACTTTT 
TATTTTCAAA AATCATATTT CAATGCAATA TACGATTTTT TATTAAAGTT GAAGCGATTT 
CAGGAATTAT TTAGCTCAAT TAGATATGCG ATCTACTATA AGCTTTACGG AAACAAAGTT 
AGCCTAACTT ATCCTAAATT TGAGCTTCGA ATTAAGCATC CAAAGGTATA CTTTGATGAT 
GTATTAGATG AAGAAAGAAA TTGTCGGATG CAATTGATAG TGAAACCCCA ATCCTTTTAT 
GCGTTTAGTA AATGTCCGAT TGTGGAAAAG AACTCGAAGA AATCAATATT CAGTTGTGAA 
ATAACTAAGG TTGAATTTCA TACTGCCGTC CCTAGTTCCT CGCATCACGA TGTACTAATG 
GAGGAAAATA ATGTTCATTT GGACCTTACA TATGACGCTA ATTACACTAC TGGTGCTTAT 
GTATTCAAAG CAACTGGTGA TTTGGATCCT GTCATATTAA ACATGTGCCA GTCACATCAT 
GTTATTTTCT GGGATTTAAT CGATGTAGCC ACTACCTTTG CTAGAGTTGA TTCTTCATTT 
TATACTTCTG AAAACCTAAG ACGAGAACTG GATAAGGCTT TTGATAGGTC CGGTACTGCT 
GCCAAATTAA AACATCCAAA AAAAACCGTA GTAGAAACTT TAGATATTCT CACTACGTTT 
AATTTACCTG AAATACGTCT CAATGTGCAC ACCGATGATT TCTGGATTCA CGGTGGGGAT 
TTAACACAAT TGCATTCCAT ATTAAGCTTC TTTGGATTCT CACTTGATTA TAATTTTTAT 
TCAAGCGGTC GTTGTTATGC TGAGTTTTCA ATCGATTCTA TACAATTAAA AGATTGCAAT 
CCTCAAGATA ATGTCGTTTT TTTGGATGTA TTAGAATACA GTGAAAATCA TAACAGACTT 
GTCAATGGTT GCCTTGAATA TGATTCTCAA AATCCAAGAT ACAACCTCGT ACTAGATATT 
GATTCACCGA AGATATTTGT CAATTTGAAT TATTTGTATT CTATTTGGTC TATATTTGTT 
CATTGGCATC GTGCTTACTA TTCGCACTTG GATTATTTAA CAGAGGTTGA ATACTTTATT 
ATGGGAAATC CAAACCAAAA TGCTTGTGGA GAAGAAAGCT ATTGGTACTA CAGAATTACA 
TTTGTTGATA TGACTTTGCT CTTTTTTAGA AACGTATCTG ATGCCAATCT TTACTCACTA 
CCAATGTTTT TTGGAGAGCT ATTAATTACC CAACAATCGA TCTTTGCGGT TACTGCTAAC 
AACATGAAGA TTAATGCTTG TCCTCTCAGC GAGACGGCCA ATATATCTAA TCAGTTAGCA 
GACCCTTTTG GATTTCGTTA CACATATTCG CAGCATACCG TCAACAAAAT TCAGATAATC 
ACCAACATCA CTCTTGATTT TGATTCGTTT GTCTTAAGGA CTACAGTGAA CGACTTTCTG 
TTCCTCCAAA CTATTTTACG TAAGATATAT AATTTTTATT ATGCCCTATA CGATGTCCCC 
ACAACCGATG TTGAATTGCT GAAGAGAACG AAGGATGATC AACTAGCGAC GAATCCCGAT 
TTTCTTCAAT TGTCAGTAGA CACCGGTCAG CCTTCTTCTG TTTTTGGAAT ACGTATATGC 
AAGGAAGAAT TTTTATTAAC AGTTGACGGT ATTCGACTAT TGGTTATTAG TCAACTACAT 
GATCTTCCAC TTTTAAATAT TAACATTAAG CCTTTTCAGG TAGATCTTAA CGATTGGTCT 
TCCGAGTTAA ATTCAAATGC ACATTTGGAG CTTTTTATGA ATTTTTATAA CTTCTCTAAC 
TCTCATTGGG AGCCCTTTTT AGAGCCCTGG AAAGTAGGTG TACATATTTC TAGGAATCCA 
AACACATCGA AGACTGCGGT ACATGTTTTT TCGAGAGAGA AATTAGATCT GGTTATTACT 
CCTCAGTTAA TTGAGACTCT ACATTTTGGT TTCACCAAAG TTATCAGTAC TCCATTTCCT 
ATTGAATTCA AATGTGACGC ACCTTATCGT ATTCACAACT ACACAGGACA TGCTGTTAGT 
GTTTGGGCAG ATTTTGAAAA TGCTGCAGAT TCTTGCGTTA GACATCTCGA AAATAACGAA 
GAAACCGACT GGAAATTTGA AGAATGGAGA CAAATGCAGG ATGTGGTTAA ACAAGATCAG 
GATAGATCTT ATATAGGGTT CCATTTCGAA AACAGTAAAT GGGAGTCTTT ACGACATGTA 
CGAGTTAATC GTGTAGGAGA GCATATATAT CCCTTAATTT CCTATGATCA AGACGAGCTA 
AAGCATTATA TGGTAGTTGA TGTCAATTTG GGAGAAGATT ATATTAAACA TATCACTCTA 
CGTTCCCCAC TTTTATTGAT AAATGAGACC CAAATGGAAA TAGATGTAGT TTTTTGCGAC 
TCAGATGGCA TTCAAAGGTC ACAGATATAC CACATGAGCC CTGAAGAGAG TTGCTCTTTG 
CCAATTGAAA CTGCTTATTA TTATTCAATT CACATTAGAC CGGTTTCAGA ATTTAAATTT 
AATTGGACTT CGGAAGCTAT ATCTTGGAAA GATCTAGTTG ACAACAAACA GAGCCTTGTG 
ACATGCCAAC ATTCAGATAA CACCTTCTCG ACTCCTGCGT GTCGTTTTGC CGCTAATGCA 
GAACTTAAAA GTCAAACTAT ATCCAACCAT TATCCTTTTA TGCATATTAC GATTTCTGCG 
CTCCTAGAAG TGAAAAACTT ATTGCCAATT GATCTTAACA TTCGAATCAT CGATAAGGAC 
CAAGAGGGAG TATGGATGTC GAATGTTGGT ATTGGAGAGT GCGCATATGT TCACTCGATA 
AATATTTCCC ACGTTTTGCT CCTACAAGCC GAGTCATCAG AGTCGCATTA CCTTCCCAGC 
TCCTTGGCTA CTATTATCAC TAACGACTCT GCTCAAGAAA GAGATGAGTA CATGACAATC 
ACTCTTCAGG GAGGTAGAAA AACAAGATTA GGGTTGAGTT ATACTGAAAA GTATCCTGGA 
ATTTATCACA TCGAAATTTT TAGCCCGTAC ATAATAATTA ACAAGTCAGG GTCCTTTCTG 
TTTGTTGGTC CAAAAAATGA TTACAACCGT ATTTCTTTTT CTTCAGCTTC TCTTTCGTCT 
GGGGAAGACG GCAAAGTTGT TCCATGTATG TTTTCATATT CGCATAACTA TGGCAGCAGA 
AGATGTCGGT TACGAGCAGA TAATTCTAAT TGGAGTGAGC CCGTTAGCTT TGATGCGATT 
GGCAGTGTGT TTGAAGTAGA ATTACCTTCC AAGGAAGACC ATAATAAAGT TTACAGATTA 
GGTATTTTTG TTGAAACGGG ACCCGATGGT TACTCGAAAA CTAATATCGT TACAATAACT 
TCTAGATTCA TAGTGAGAAA TAAAACAAGA TGGAGTTTGG TGATTGCGGA GCCTTACAAC 
GATTTTATTG CGGAAATCGC TCCTGAGGGT GAAGAGTTTT TGACATATTT AAGAAAGCAT 
TCACACCCGA TGTTAAAGCT ATCCTCCTCA GATTGCTACC TTTGGTCATC ATCATTTTAT 
ATTGAGGAGA TAGGGTCAAC TCATGTACGT TTAATGACCT CGGAAGGGGA AAAACTTTTA 
CGCTTGGAAA TTGTCATAAA AAATGCCACA ATCTTTATCA GTATATTTGA AGAGACTGGG 
GACTGGCCAT ACTATATAAA AAATGAATCT GGTGTTTTGT TAAAATTTTG GCAGGTAAAT 
CCTATTGATG CCTCAGAAGG AAAGAATAAT ACTGCTTTGT TGAAGTACCA TGATATACCG 
CCTCATTCAG AGGTAAAGTA TTCTTGGGAT TATCCCTGTT GTGCTAATAA GGAAATTGCT 
TTATGCTATG GTGATCAAAA ATGCTTAACT ACTTTGGCAG AAATTGGCCC CCTTTCTCCC 
TTTAAATTCA CCGATGCTTC CAATAATACA AAATTTATTT CACGAGATAT AGTCGCTAAT 
GGGCTTTCGA AAATTCTTAT TCTAAAAGAC TATGATCCTT CAAAGGCTGT GAGGAAGCCG 
AAGATATACA GCAAAGTCTC AACTGAAGAA CGCGATTTTA ATTTGGAGCA ATTTGACTCC 
GGGATTGACT TGTCCGTCAA ATTTTTGCTG GAGGGAATTG GGATATCTTT GGTAGAACGG 
AATACGCAAG AATTAGCGTA TTTGACCTTT CATGGGATCA ACTTGTTCTT TACAGATTCC 
CATCTTATAC GCACTTTTAA ATTAGATGTT CGATGGATAC AAATTGACAA CCAGCTTTAT 
GGAGGAATTT ATCCAATTAT CCTCTATCCA AGTATTCTAT CACAGGAAGA CACAATGAAT 
GACAATTCAC TTTTACCAAC GTTTCACTCA ATGGTTGCAG TTGTCAAGAA TGATACATAC 
GGTGTGACTT ACGTAAAATA TGCTACTATA TTACTTCAGG AATTAACTAT TGAAATTGAT 
GAGGACTTTG CTTTTGCTGC ACTCGAATAC ATCAAGGACT CTGTGCCCCG AAGCAAAAGA 
AATACCGGCA AGATGTTTGA TGATTCGCTG GAACTTGTAC CAGAAAATCT AGGAAATGAT 
TTGAAAGTGT ATTTTGAAGT GCTTAATTTG CAACCCACTG AAATGCATCT GTCTTTCGTT 
CGTACGGAGA GGATCAATAA TACTGATGGC ACCGTTGTCT CTTCACATAA TCCATTTGTC 
TTTTTCGTGA ATGTTTTATC AATGGCAATA GGAAACATAA ATGATGCACC AGTCCGTTTA 
AATGCTCTTC TCATGGATAA TGCACACGTT TCTCTCCGCC GTCTTTTTGA ACTTGTCAAA 
AACCATTATT CTCAAGAACT TTTATCCCAA GTGCATAAGA TTGTTGGATC AGCAGACTTT 
TTGGGAAATC CTGTTGGCTT ATTTACCACA ATAACGTCCG GTTTTGCTGA TATATTTTAT 
GAACCCTTTC ATGGTTTTAT CTTAAACGAG GGTTCTTATG AATTAGGTAT AGGATTTGCC 
AAGGGTACTG CAAGCTTTAT TAAAAAGGCA GTATTCGGAA TCACTGACAG TATTTCAAAA 
GTAACGGGCA CTATTTCAAG AAGCTTAAGT GTGATCACAT TGGATCCCAA ATTCCAAAGC 
CGAAGACGTG CTGCTCGTAT CAGAAATCGG CCTGTTCATA TACTTTATGG AGTGACAGCT 
GGAGCTGCGT CACTTTACAC AGGAGTTCGA AGTGGTGTCA GAGGATTAGC CTTACAACCT 
ATTATTGGAG CAAGGAGAAA TGGACTTCCT GGCCTTGTTA AAGGCTTAGG TAAGGGTTTG 
GTGGGTTTCA CGACAAAACC ATTGGTAGGG TTATTCGATT TTGCGTCAAG TATCTCTGAA 
GGCGCAAGAA ATACAACAAC TGTGTTTGAT GAACGACACA TAGAAAAGCT AAGATTGTCA 
AGACTGATGA GTGATGATGG TGTCGTATAT CCTTTTCAAC TACGGGAGGC TTTGGGACAG 
TACTGGCTAA AACATCTCGA TAATGGAAGA TATTTTAAAG ATTTTTATAA GGCTCATATC 
ATTATTGAAA ATAAAGTGCT TGTGATCTTA ACGAATAATA GGATATTGTT TGTTCAGCCT 
CAACAGCTTA ATTGCAAAAA AGAAATTCAT CTCTCCAAAG TTAAAACTGT TAAGTTACAG 
AGCAAAGAGC ATATCTTTCT CCAACTTTTG AAGGGTGTAA ACTTTGAATT TTCTGTCCCC 
GAAAACTCCG TTCGGACATT TTTCTACCGA AAAATTCGAG ACGAGCTTGC CGCTTATAAA 
CACAAAGTAA ATTACGAGCT AGAGGTTGCT CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001713147.1
CDS	73..9288
Translation	MLEGLVAGLLNKILGSYVDNLDTKQLNIGVWGGHVSLHNLRIKPEALDKLGIPIEITSGLIGTFTLEIPWSNLRNKSLTINIEDIYLSIHPQAKNSLTRDELEQSQQALKQEQLDSFEILRKNFRETLEESSSNPNISRKQSFIEYLIAKLTDNIQIYIERIHLRFEDNLSDLEKPYSLGLTLYSLRVTSTDASFTEYLLSTDPIPSSCIHKIITVDYFSIYWISKCEISKCTTTEDIFSYLKNLIPSAEKSPAYNYILKPLRATAHVVLFRHPTDQIMQLRGKLSVEEISITLSDHMYYSLLGVIDYFRVVMKQQYYLQYRPKSTPKEKPLEWFKYAILVVKDSVHESRYHWTWKYFKRRRDDRIAYMHIIRKRYLNEQISKEEIDLQKKIEKRNSTYDLIKYRSRVHTSLIEERNSIYLKPKTSAAHGLYDWFSGYIRKPQSQDEDTLASTDKTAADLTDQEQKEFFSAIEWSGQLYPDTVNLDPDMCMANVEVSIAKGSFVIQSHINGRVIPLIKQRFESFATECFIRPQSLKLKVSLKDLDMFDGITNPELEPARVIFAKPSVEESESLQKIPEAYRTHLFFLLLDTKPVYKASSTLIVHLRTLVIIYNRVCIESLLAFFVPPRTKIEHVSEWGYSAAAKVMTLARQTRASLDYALEMHKTSDMTIDLQAPLIVVREECTDLKSPTLFLDVGRALVHTQLVDDAIIDKFRKLQSKKINNEQLKQLENLMYDKFTISLFNVRCLIGPDYETGWRCLPKGCDYHILKECSLDINFEISILQKATNLTKFKVSSHMKHAEIMFSDVQYKVFINMMSNILPTLPVAEIPFTYQQFLDAVKPPPFFDAPDNFQITHTSLGSHANENTAAQFMAQQIFAFYFKVDYAICSLYRRSENYLIPVVRAFTEFYIDLVVRKFDYLVTSKLNDLVIKEFTYPSSLCDNVLVRSSPSPKNNFDDTVFISYTSIDYDSPELDSVYEGVRTTIAVVLSDLILNVEPTGFSFVYDFIRATFTSLNDEYMIGEDPELTRKISPVEGIIPEDANVRFDNVDIFLYDCDQHFSTVCLYSANMHMEFREKFFLQARFYDLEVKNHMKSNNPPKTIVKIDDNDLFIFKYESYDIPKDISKPTCDCVYDISFGSLTFYFQKSYFNAIYDFLLKLKRFQELFSSIRYAIYYKLYGNKVSLTYPKFELRIKHPKVYFDDVLDEERNCRMQLIVKPQSFYAFSKCPIVEKNSKKSIFSCEITKVEFHTAVPSSSHHDVLMEENNVHLDLTYDANYTTGAYVFKATGDLDPVILNMCQSHHVIFWDLIDVATTFARVDSSFYTSENLRRELDKAFDRSGTAAKLKHPKKTVVETLDILTTFNLPEIRLNVHTDDFWIHGGDLTQLHSILSFFGFSLDYNFYSSGRCYAEFSIDSIQLKDCNPQDNVVFLDVLEYSENHNRLVNGCLEYDSQNPRYNLVLDIDSPKIFVNLNYLYSIWSIFVHWHRAYYSHLDYLTEVEYFIMGNPNQNACGEESYWYYRITFVDMTLLFFRNVSDANLYSLPMFFGELLITQQSIFAVTANNMKINACPLSETANISNQLADPFGFRYTYSQHTVNKIQIITNITLDFDSFVLRTTVNDFLFLQTILRKIYNFYYALYDVPTTDVELLKRTKDDQLATNPDFLQLSVDTGQPSSVFGIRICKEEFLLTVDGIRLLVISQLHDLPLLNINIKPFQVDLNDWSSELNSNAHLELFMNFYNFSNSHWEPFLEPWKVGVHISRNPNTSKTAVHVFSREKLDLVITPQLIETLHFGFTKVISTPFPIEFKCDAPYRIHNYTGHAVSVWADFENAADSCVRHLENNEETDWKFEEWRQMQDVVKQDQDRSYIGFHFENSKWESLRHVRVNRVGEHIYPLISYDQDELKHYMVVDVNLGEDYIKHITLRSPLLLINETQMEIDVVFCDSDGIQRSQIYHMSPEESCSLPIETAYYYSIHIRPVSEFKFNWTSEAISWKDLVDNKQSLVTCQHSDNTFSTPACRFAANAELKSQTISNHYPFMHITISALLEVKNLLPIDLNIRIIDKDQEGVWMSNVGIGECAYVHSINISHVLLLQAESSESHYLPSSLATIITNDSAQERDEYMTITLQGGRKTRLGLSYTEKYPGIYHIEIFSPYIIINKSGSFLFVGPKNDYNRISFSSASLSSGEDGKVVPCMFSYSHNYGSRRCRLRADNSNWSEPVSFDAIGSVFEVELPSKEDHNKVYRLGIFVETGPDGYSKTNIVTITSRFIVRNKTRWSLVIAEPYNDFIAEIAPEGEEFLTYLRKHSHPMLKLSSSDCYLWSSSFYIEEIGSTHVRLMTSEGEKLLRLEIVIKNATIFISIFEETGDWPYYIKNESGVLLKFWQVNPIDASEGKNNTALLKYHDIPPHSEVKYSWDYPCCANKEIALCYGDQKCLTTLAEIGPLSPFKFTDASNNTKFISRDIVANGLSKILILKDYDPSKAVRKPKIYSKVSTEERDFNLEQFDSGIDLSVKFLLEGIGISLVERNTQELAYLTFHGINLFFTDSHLIRTFKLDVRWIQIDNQLYGGIYPIILYPSILSQEDTMNDNSLLPTFHSMVAVVKNDTYGVTYVKYATILLQELTIEIDEDFAFAALEYIKDSVPRSKRNTGKMFDDSLELVPENLGNDLKVYFEVLNLQPTEMHLSFVRTERINNTDGTVVSSHNPFVFFVNVLSMAIGNINDAPVRLNALLMDNAHVSLRRLFELVKNHYSQELLSQVHKIVGSADFLGNPVGLFTTITSGFADIFYEPFHGFILNEGSYELGIGFAKGTASFIKKAVFGITDSISKVTGTISRSLSVITLDPKFQSRRRAARIRNRPVHILYGVTAGAASLYTGVRSGVRGLALQPIIGARRNGLPGLVKGLGKGLVGFTTKPLVGLFDFASSISEGARNTTTVFDERHIEKLRLSRLMSDDGVVYPFQLREALGQYWLKHLDNGRYFKDFYKAHIIIENKVLVILTNNRILFVQPQQLNCKKEIHLSKVKTVKLQSKEHIFLQLLKGVNFEFSVPENSVRTFFYRKIRDELAAYKHKVNYELEVAL

Target ORF information:

RefSeq Version	XM_001713095.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- vacuolar protein sorting-associated protein (vps13a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001713095.2

ORF Insert Sequence:

ATGTTAGAAG GCCTAGTTGC CGGCCTCTTG AACAAAATTT TAGGTTCGTA TGTTGACAAC 
TTGGATACTA AACAACTAAA CATCGGCGTT TGGGGTGGCC ATGTTAGTTT GCATAATTTA 
CGAATAAAAC CTGAGGCCTT AGATAAACTC GGAATTCCCA TTGAAATCAC TAGTGGTTTA 
ATTGGCACGT TTACGCTGGA AATTCCATGG TCGAATCTTC GGAACAAATC TTTAACGATC 
AATATTGAAG ACATATATTT GTCAATTCAT CCCCAAGCGA AAAACTCTTT AACACGCGAT 
GAGTTGGAGC AATCTCAACA AGCTTTAAAG CAGGAACAGC TGGATAGTTT TGAAATCTTA 
CGGAAAAATT TTCGAGAAAC TTTAGAAGAG TCTTCATCAA ATCCAAATAT AAGTAGAAAA 
CAATCTTTTA TTGAGTATCT TATTGCCAAA CTTACTGATA ACATTCAAAT ATACATCGAA 
AGAATTCACT TGCGGTTTGA AGATAATTTG AGTGATCTTG AAAAACCTTA TTCTCTTGGA 
TTGACGCTTT ATTCGTTAAG AGTAACCTCT ACAGATGCTA GTTTTACCGA ATATTTGCTT 
TCAACCGATC CAATTCCTTC CTCTTGCATT CATAAAATTA TTACTGTTGA TTATTTCTCA 
ATATACTGGA TAAGTAAGTG TGAAATTTCT AAATGCACTA CTACAGAAGA CATATTCTCT 
TATTTAAAGA ACCTGATTCC TAGTGCAGAA AAGTCACCTG CTTATAATTA CATTTTAAAG 
CCGCTACGGG CAACCGCCCA TGTTGTCTTA TTTCGACATC CGACAGACCA GATAATGCAA 
TTAAGGGGAA AATTATCTGT TGAAGAAATA TCAATTACGC TAAGTGATCA TATGTATTAC 
AGTTTACTTG GTGTTATTGA CTACTTTCGT GTTGTAATGA AGCAACAATA TTATCTTCAA 
TATAGGCCTA AATCGACTCC TAAAGAGAAA CCATTGGAGT GGTTTAAGTA TGCCATTTTA 
GTTGTTAAAG ATTCTGTTCA TGAGAGCAGA TATCACTGGA CTTGGAAATA CTTTAAAAGG 
AGAAGGGACG ACAGAATAGC TTACATGCAT ATTATTCGTA AGAGGTATTT GAATGAGCAA 
ATATCAAAGG AAGAAATTGA TTTGCAAAAA AAGATAGAGA AACGAAATTC TACTTACGAT 
CTAATTAAGT ACCGTTCACG TGTGCATACA TCTCTGATTG AGGAAAGGAA TTCTATTTAT 
TTAAAACCAA AAACTTCGGC AGCTCATGGA TTGTACGATT GGTTTTCTGG GTATATACGA 
AAGCCGCAGT CCCAGGATGA AGATACATTA GCAAGTACTG ATAAAACAGC CGCTGACTTG 
ACTGATCAAG AACAGAAAGA ATTTTTTTCT GCAATAGAAT GGAGCGGACA ATTATATCCT 
GATACCGTAA ATTTAGATCC GGATATGTGT ATGGCTAATG TTGAAGTCTC TATCGCTAAA 
GGAAGTTTTG TCATACAATC TCACATTAAT GGACGAGTAA TACCTTTAAT TAAGCAAAGG 
TTTGAGAGTT TTGCAACAGA ATGTTTTATA AGGCCTCAAT CACTGAAATT GAAAGTGTCT 
CTTAAGGATT TGGATATGTT TGATGGCATA ACGAACCCCG AGCTTGAGCC TGCTCGTGTA 
ATTTTTGCAA AGCCTTCTGT TGAAGAAAGT GAAAGTTTGC AAAAAATCCC TGAAGCCTAC 
AGAACACATT TGTTTTTTTT ATTGTTAGAC ACCAAACCGG TGTATAAAGC GAGTTCTACT 
CTAATCGTAC ATTTGCGAAC TTTGGTTATT ATTTACAATC GAGTTTGTAT TGAAAGTCTG 
CTAGCTTTTT TTGTTCCTCC AAGAACAAAA ATCGAGCACG TTTCTGAATG GGGATATTCT 
GCAGCTGCAA AAGTTATGAC CCTAGCTAGA CAAACTCGTG CTAGTTTGGA TTATGCCCTA 
GAAATGCACA AGACTTCCGA TATGACAATT GACCTGCAAG CTCCCTTGAT TGTTGTTCGA 
GAAGAATGTA CGGATTTAAA AAGCCCTACA CTGTTCCTTG ACGTGGGTAG AGCGTTGGTA 
CATACCCAAT TAGTTGATGA TGCGATTATC GACAAATTCA GAAAACTCCA GAGCAAAAAG 
ATAAACAACG AGCAATTAAA ACAACTAGAA AACTTGATGT ACGACAAGTT TACCATCTCA 
TTATTTAATG TTCGATGCTT AATTGGGCCT GATTATGAAA CTGGTTGGCG TTGCTTACCC 
AAAGGGTGTG ATTACCATAT TTTGAAAGAA TGCTCTCTTG ACATTAACTT TGAAATATCA 
ATCCTTCAAA AAGCTACTAA CTTAACGAAG TTCAAAGTTT CAAGTCATAT GAAGCATGCC 
GAAATTATGT TTTCTGATGT GCAATATAAG GTATTCATTA ATATGATGAG CAATATATTG 
CCTACGCTTC CTGTTGCTGA AATACCATTT ACATACCAAC AATTCCTTGA CGCGGTTAAG 
CCTCCTCCTT TCTTTGATGC TCCTGATAAT TTTCAAATAA CTCATACTTC TCTTGGAAGT 
CATGCTAATG AAAACACCGC TGCGCAATTT ATGGCCCAGC AAATTTTTGC TTTCTATTTT 
AAGGTGGATT ATGCTATATG TTCGTTGTAC CGAAGATCTG AAAACTATCT CATACCTGTG 
GTAAGAGCAT TTACAGAGTT TTACATCGAC CTAGTAGTGC GAAAATTTGA TTACCTTGTT 
ACTTCCAAGC TTAACGACTT GGTTATTAAA GAATTTACAT ATCCTTCTTC TCTTTGTGAC 
AATGTTTTAG TTCGTTCTTC TCCTTCACCT AAAAATAACT TTGATGATAC CGTTTTCATC 
AGCTATACAA GCATTGATTA TGACTCTCCT GAACTAGATT CTGTTTACGA AGGTGTTCGA 
ACTACCATTG CTGTAGTCCT TTCTGATCTC ATTCTCAACG TTGAGCCTAC TGGATTTTCA 
TTTGTCTACG ATTTTATCAG AGCCACTTTT ACCTCTTTAA ATGATGAGTA TATGATTGGT 
GAAGATCCAG AGTTGACTCG AAAAATTTCA CCAGTTGAAG GAATTATTCC AGAAGACGCC 
AACGTTCGGT TTGACAACGT AGATATTTTT CTTTATGACT GTGATCAACA TTTTTCAACA 
GTTTGTCTGT ATAGTGCTAA CATGCATATG GAATTCAGAG AGAAGTTTTT CCTTCAAGCA 
CGATTTTATG ACCTTGAAGT TAAAAACCAT ATGAAAAGTA ATAATCCGCC AAAGACCATT 
GTGAAGATTG ACGATAATGA TTTATTTATA TTTAAATATG AGAGTTATGA CATTCCAAAA 
GACATATCGA AACCTACATG CGACTGTGTT TATGATATAT CTTTTGGCTC ATTAACTTTT 
TATTTTCAAA AATCATATTT CAATGCAATA TACGATTTTT TATTAAAGTT GAAGCGATTT 
CAGGAATTAT TTAGCTCAAT TAGATATGCG ATCTACTATA AGCTTTACGG AAACAAAGTT 
AGCCTAACTT ATCCTAAATT TGAGCTTCGA ATTAAGCATC CAAAGGTATA CTTTGATGAT 
GTATTAGATG AAGAAAGAAA TTGTCGGATG CAATTGATAG TGAAACCCCA ATCCTTTTAT 
GCGTTTAGTA AATGTCCGAT TGTGGAAAAG AACTCGAAGA AATCAATATT CAGTTGTGAA 
ATAACTAAGG TTGAATTTCA TACTGCCGTC CCTAGTTCCT CGCATCACGA TGTACTAATG 
GAGGAAAATA ATGTTCATTT GGACCTTACA TATGACGCTA ATTACACTAC TGGTGCTTAT 
GTATTCAAAG CAACTGGTGA TTTGGATCCT GTCATATTAA ACATGTGCCA GTCACATCAT 
GTTATTTTCT GGGATTTAAT CGATGTAGCC ACTACCTTTG CTAGAGTTGA TTCTTCATTT 
TATACTTCTG AAAACCTAAG ACGAGAACTG GATAAGGCTT TTGATAGGTC CGGTACTGCT 
GCCAAATTAA AACATCCAAA AAAAACCGTA GTAGAAACTT TAGATATTCT CACTACGTTT 
AATTTACCTG AAATACGTCT CAATGTGCAC ACCGATGATT TCTGGATTCA CGGTGGGGAT 
TTAACACAAT TGCATTCCAT ATTAAGCTTC TTTGGATTCT CACTTGATTA TAATTTTTAT 
TCAAGCGGTC GTTGTTATGC TGAGTTTTCA ATCGATTCTA TACAATTAAA AGATTGCAAT 
CCTCAAGATA ATGTCGTTTT TTTGGATGTA TTAGAATACA GTGAAAATCA TAACAGACTT 
GTCAATGGTT GCCTTGAATA TGATTCTCAA AATCCAAGAT ACAACCTCGT ACTAGATATT 
GATTCACCGA AGATATTTGT CAATTTGAAT TATTTGTATT CTATTTGGTC TATATTTGTT 
CATTGGCATC GTGCTTACTA TTCGCACTTG GATTATTTAA CAGAGGTTGA ATACTTTATT 
ATGGGAAATC CAAACCAAAA TGCTTGTGGA GAAGAAAGCT ATTGGTACTA CAGAATTACA 
TTTGTTGATA TGACTTTGCT CTTTTTTAGA AACGTATCTG ATGCCAATCT TTACTCACTA 
CCAATGTTTT TTGGAGAGCT ATTAATTACC CAACAATCGA TCTTTGCGGT TACTGCTAAC 
AACATGAAGA TTAATGCTTG TCCTCTCAGC GAGACGGCCA ATATATCTAA TCAGTTAGCA 
GACCCTTTTG GATTTCGTTA CACATATTCG CAGCATACCG TCAACAAAAT TCAGATAATC 
ACCAACATCA CTCTTGATTT TGATTCGTTT GTCTTAAGGA CTACAGTGAA CGACTTTCTG 
TTCCTCCAAA CTATTTTACG TAAGATATAT AATTTTTATT ATGCCCTATA CGATGTCCCC 
ACAACCGATG TTGAATTGCT GAAGAGAACG AAGGATGATC AACTAGCGAC GAATCCCGAT 
TTTCTTCAAT TGTCAGTAGA CACCGGTCAG CCTTCTTCTG TTTTTGGAAT ACGTATATGC 
AAGGAAGAAT TTTTATTAAC AGTTGACGGT ATTCGACTAT TGGTTATTAG TCAACTACAT 
GATCTTCCAC TTTTAAATAT TAACATTAAG CCTTTTCAGG TAGATCTTAA CGATTGGTCT 
TCCGAGTTAA ATTCAAATGC ACATTTGGAG CTTTTTATGA ATTTTTATAA CTTCTCTAAC 
TCTCATTGGG AGCCCTTTTT AGAGCCCTGG AAAGTAGGTG TACATATTTC TAGGAATCCA 
AACACATCGA AGACTGCGGT ACATGTTTTT TCGAGAGAGA AATTAGATCT GGTTATTACT 
CCTCAGTTAA TTGAGACTCT ACATTTTGGT TTCACCAAAG TTATCAGTAC TCCATTTCCT 
ATTGAATTCA AATGTGACGC ACCTTATCGT ATTCACAACT ACACAGGACA TGCTGTTAGT 
GTTTGGGCAG ATTTTGAAAA TGCTGCAGAT TCTTGCGTTA GACATCTCGA AAATAACGAA 
GAAACCGACT GGAAATTTGA AGAATGGAGA CAAATGCAGG ATGTGGTTAA ACAAGATCAG 
GATAGATCTT ATATAGGGTT CCATTTCGAA AACAGTAAAT GGGAGTCTTT ACGACATGTA 
CGAGTTAATC GTGTAGGAGA GCATATATAT CCCTTAATTT CCTATGATCA AGACGAGCTA 
AAGCATTATA TGGTAGTTGA TGTCAATTTG GGAGAAGATT ATATTAAACA TATCACTCTA 
CGTTCCCCAC TTTTATTGAT AAATGAGACC CAAATGGAAA TAGATGTAGT TTTTTGCGAC 
TCAGATGGCA TTCAAAGGTC ACAGATATAC CACATGAGCC CTGAAGAGAG TTGCTCTTTG 
CCAATTGAAA CTGCTTATTA TTATTCAATT CACATTAGAC CGGTTTCAGA ATTTAAATTT 
AATTGGACTT CGGAAGCTAT ATCTTGGAAA GATCTAGTTG ACAACAAACA GAGCCTTGTG 
ACATGCCAAC ATTCAGATAA CACCTTCTCG ACTCCTGCGT GTCGTTTTGC CGCTAATGCA 
GAACTTAAAA GTCAAACTAT ATCCAACCAT TATCCTTTTA TGCATATTAC GATTTCTGCG 
CTCCTAGAAG TGAAAAACTT ATTGCCAATT GATCTTAACA TTCGAATCAT CGATAAGGAC 
CAAGAGGGAG TATGGATGTC GAATGTTGGT ATTGGAGAGT GCGCATATGT TCACTCGATA 
AATATTTCCC ACGTTTTGCT CCTACAAGCC GAGTCATCAG AGTCGCATTA CCTTCCCAGC 
TCCTTGGCTA CTATTATCAC TAACGACTCT GCTCAAGAAA GAGATGAGTA CATGACAATC 
ACTCTTCAGG GAGGTAGAAA AACAAGATTA GGGTTGAGTT ATACTGAAAA GTATCCTGGA 
ATTTATCACA TCGAAATTTT TAGCCCGTAC ATAATAATTA ACAAGTCAGG GTCCTTTCTG 
TTTGTTGGTC CAAAAAATGA TTACAACCGT ATTTCTTTTT CTTCAGCTTC TCTTTCGTCT 
GGGGAAGACG GCAAAGTTGT TCCATGTATG TTTTCATATT CGCATAACTA TGGCAGCAGA 
AGATGTCGGT TACGAGCAGA TAATTCTAAT TGGAGTGAGC CCGTTAGCTT TGATGCGATT 
GGCAGTGTGT TTGAAGTAGA ATTACCTTCC AAGGAAGACC ATAATAAAGT TTACAGATTA 
GGTATTTTTG TTGAAACGGG ACCCGATGGT TACTCGAAAA CTAATATCGT TACAATAACT 
TCTAGATTCA TAGTGAGAAA TAAAACAAGA TGGAGTTTGG TGATTGCGGA GCCTTACAAC 
GATTTTATTG CGGAAATCGC TCCTGAGGGT GAAGAGTTTT TGACATATTT AAGAAAGCAT 
TCACACCCGA TGTTAAAGCT ATCCTCCTCA GATTGCTACC TTTGGTCATC ATCATTTTAT 
ATTGAGGAGA TAGGGTCAAC TCATGTACGT TTAATGACCT CGGAAGGGGA AAAACTTTTA 
CGCTTGGAAA TTGTCATAAA AAATGCCACA ATCTTTATCA GTATATTTGA AGAGACTGGG 
GACTGGCCAT ACTATATAAA AAATGAATCT GGTGTTTTGT TAAAATTTTG GCAGGTAAAT 
CCTATTGATG CCTCAGAAGG AAAGAATAAT ACTGCTTTGT TGAAGTACCA TGATATACCG 
CCTCATTCAG AGGTAAAGTA TTCTTGGGAT TATCCCTGTT GTGCTAATAA GGAAATTGCT 
TTATGCTATG GTGATCAAAA ATGCTTAACT ACTTTGGCAG AAATTGGCCC CCTTTCTCCC 
TTTAAATTCA CCGATGCTTC CAATAATACA AAATTTATTT CACGAGATAT AGTCGCTAAT 
GGGCTTTCGA AAATTCTTAT TCTAAAAGAC TATGATCCTT CAAAGGCTGT GAGGAAGCCG 
AAGATATACA GCAAAGTCTC AACTGAAGAA CGCGATTTTA ATTTGGAGCA ATTTGACTCC 
GGGATTGACT TGTCCGTCAA ATTTTTGCTG GAGGGAATTG GGATATCTTT GGTAGAACGG 
AATACGCAAG AATTAGCGTA TTTGACCTTT CATGGGATCA ACTTGTTCTT TACAGATTCC 
CATCTTATAC GCACTTTTAA ATTAGATGTT CGATGGATAC AAATTGACAA CCAGCTTTAT 
GGAGGAATTT ATCCAATTAT CCTCTATCCA AGTATTCTAT CACAGGAAGA CACAATGAAT 
GACAATTCAC TTTTACCAAC GTTTCACTCA ATGGTTGCAG TTGTCAAGAA TGATACATAC 
GGTGTGACTT ACGTAAAATA TGCTACTATA TTACTTCAGG AATTAACTAT TGAAATTGAT 
GAGGACTTTG CTTTTGCTGC ACTCGAATAC ATCAAGGACT CTGTGCCCCG AAGCAAAAGA 
AATACCGGCA AGATGTTTGA TGATTCGCTG GAACTTGTAC CAGAAAATCT AGGAAATGAT 
TTGAAAGTGT ATTTTGAAGT GCTTAATTTG CAACCCACTG AAATGCATCT GTCTTTCGTT 
CGTACGGAGA GGATCAATAA TACTGATGGC ACCGTTGTCT CTTCACATAA TCCATTTGTC 
TTTTTCGTGA ATGTTTTATC AATGGCAATA GGAAACATAA ATGATGCACC AGTCCGTTTA 
AATGCTCTTC TCATGGATAA TGCACACGTT TCTCTCCGCC GTCTTTTTGA ACTTGTCAAA 
AACCATTATT CTCAAGAACT TTTATCCCAA GTGCATAAGA TTGTTGGATC AGCAGACTTT 
TTGGGAAATC CTGTTGGCTT ATTTACCACA ATAACGTCCG GTTTTGCTGA TATATTTTAT 
GAACCCTTTC ATGGTTTTAT CTTAAACGAG GGTTCTTATG AATTAGGTAT AGGATTTGCC 
AAGGGTACTG CAAGCTTTAT TAAAAAGGCA GTATTCGGAA TCACTGACAG TATTTCAAAA 
GTAACGGGCA CTATTTCAAG AAGCTTAAGT GTGATCACAT TGGATCCCAA ATTCCAAAGC 
CGAAGACGTG CTGCTCGTAT CAGAAATCGG CCTGTTCATA TACTTTATGG AGTGACAGCT 
GGAGCTGCGT CACTTTACAC AGGAGTTCGA AGTGGTGTCA GAGGATTAGC CTTACAACCT 
ATTATTGGAG CAAGGAGAAA TGGACTTCCT GGCCTTGTTA AAGGCTTAGG TAAGGGTTTG 
GTGGGTTTCA CGACAAAACC ATTGGTAGGG TTATTCGATT TTGCGTCAAG TATCTCTGAA 
GGCGCAAGAA ATACAACAAC TGTGTTTGAT GAACGACACA TAGAAAAGCT AAGATTGTCA 
AGACTGATGA GTGATGATGG TGTCGTATAT CCTTTTCAAC TACGGGAGGC TTTGGGACAG 
TACTGGCTAA AACATCTCGA TAATGGAAGA TATTTTAAAG ATTTTTATAA GGCTCATATC 
ATTATTGAAA ATAAAGTGCT TGTGATCTTA ACGAATAATA GGATATTGTT TGTTCAGCCT 
CAACAGCTTA ATTGCAAAAA AGAAATTCAT CTCTCCAAAG TTAAAACTGT TAAGTTACAG 
AGCAAAGAGC ATATCTTTCT CCAACTTTTG AAGGGTGTAA ACTTTGAATT TTCTGTCCCC 
GAAAACTCCG TTCGGACATT TTTCTACCGA AAAATTCGAG ACGAGCTTGC CGCTTATAAA 
CACAAAGTAA ATTACGAGCT AGAGGTTGCT CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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vps13a cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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