ORF » Species Summary » Schizosaccharomyces pombe » ppk25 cDNA ORF clone

ppk25 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following ppk25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppk25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc100489 NM_001022579.2
Latest version!		 Schizosaccharomyces pombe putative serine/threonine protein kinase Ppk25 (ppk25), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    ppk25 ( NM_001022579.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596657.1 Schizosaccharomyces pombe putative serine/threonine protein kinase Ppk25 (ppk25), mRNA.
    Price & Availability↑
    CloneID OSc100489
    Clone ID Related Accession (Same CDS sequence) NM_001022579.2
    Accession Version NM_001022579.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1272bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative serine/threonine protein kinase Ppk25
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022579.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAACCGA ACACAACTAA CCTAAGGAAT GAGTGCTGGG ATACTTTCTC TATTCCTAAA 
    CGCTCTCAGA ATATAAAAAT AAATCAGTCT ACAAAACATC AAAGGTCTAT ATCTGATTTC 
    GTTGGCACAG CTGGTCCTGG TCGACAAGTT GGAAACTGGA TTATCAAGAA AACAATTGGT 
    GCTGGCAGTA TGGGAAAGGT TAAACTCGTT GTAAATATTT TAACAGGAGA GAAAGCAGCT 
    CTCAAAATGA TTCCCTTTAC TCCTAATAAT ACAAGTCAGA CAGTTCGTGT TCAAAGGGAG 
    GCACTTTTAG GTCGTTTATT GAGACATCCC AACATATGCC GGGTAATAGA CTGTATCCGG 
    ACGCCTGCAT GTACTTACAT ACTGTTCGAA TACGTTCCTG GAGGACAACT TTTGGAGTAC 
    ATCCTTGCTC GTGGCAAACT CGATGAAGAT TTGGCTAGGA GTTTTGCTAT GCAGTTGATT 
    AATGCATTGG TTTATCTTCA CAAGAATTTT ATTGTACACA GAGACTTAAA AATTGAGAAT 
    GTTCTTTTAA CTCAGGATTC TCGTCAGGTG AAGCTAATTG ACTTTGGCCT CAGTAATTTT 
    TATAGTAAAG ACGATTTATT ACGTACATAC TGTGGGAGTC TTTATTTTGC GGCCCCAGAA 
    TTACTTGATG CAAAGCCGTA CATAGGCCCA GAGGTTGATG TTTGGAGCCT GGGAGTAGTG 
    ATTTATGTTA TGGTTTGTGG ACGAGTCCCA TTTGACGACG TTTCCGTTCC TATGCTCCAT 
    TCAAAAATAA AGTCGGGTAA ACTGGAGTTT CCTTCTTACA TTTCAGAAGA TTGTTGTTCT 
    CTTATTGCAG CTATGCTGAA TGTAAACCCA CGGAAGCGCT GTTCTTTAGA ACAAGCAGCA 
    AAATTTCCCT GGTTAAAAAA AAATTCGTTT TGTTTGTATC TGCCTATTCC CTTAACCTCT 
    ATTCCCTCAA CGCCTTCAAT CCGATCTCAT GTTTTCAAAC CGCCTTTTAA TTTAAAGGTT 
    TTACAGCTGT TACATGAGCA TGGTTTAGCA AGTATTCCCG AACTAAAACA TGAACTTTAC 
    ATGGCTTACA TTGAGAGAAA AACAACTTCA TTAGTCTGTC TGTATTTATT GGGTGTCGAA 
    AGTCTCGCTC CAGCACTTCG AATTCCAACG GCTTTACCTC CTGTATATAG TAGACACCAA 
    CGGCATCATT CAGAAATTCT GGGTGCGATG GATTTGACAG AGAAAATTAC TGCTATGCAG 
    TGTCCACCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_596657.1
    CDS297..1568
    Translation

    Target ORF information:

    RefSeq Version NM_001022579.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative serine/threonine protein kinase Ppk25 (ppk25), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001022579.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAACCGA ACACAACTAA CCTAAGGAAT GAGTGCTGGG ATACTTTCTC TATTCCTAAA 
    CGCTCTCAGA ATATAAAAAT AAATCAGTCT ACAAAACATC AAAGGTCTAT ATCTGATTTC 
    GTTGGCACAG CTGGTCCTGG TCGACAAGTT GGAAACTGGA TTATCAAGAA AACAATTGGT 
    GCTGGCAGTA TGGGAAAGGT TAAACTCGTT GTAAATATTT TAACAGGAGA GAAAGCAGCT 
    CTCAAAATGA TTCCCTTTAC TCCTAATAAT ACAAGTCAGA CAGTTCGTGT TCAAAGGGAG 
    GCACTTTTAG GTCGTTTATT GAGACATCCC AACATATGCC GGGTAATAGA CTGTATCCGG 
    ACGCCTGCAT GTACTTACAT ACTGTTCGAA TACGTTCCTG GAGGACAACT TTTGGAGTAC 
    ATCCTTGCTC GTGGCAAACT CGATGAAGAT TTGGCTAGGA GTTTTGCTAT GCAGTTGATT 
    AATGCATTGG TTTATCTTCA CAAGAATTTT ATTGTACACA GAGACTTAAA AATTGAGAAT 
    GTTCTTTTAA CTCAGGATTC TCGTCAGGTG AAGCTAATTG ACTTTGGCCT CAGTAATTTT 
    TATAGTAAAG ACGATTTATT ACGTACATAC TGTGGGAGTC TTTATTTTGC GGCCCCAGAA 
    TTACTTGATG CAAAGCCGTA CATAGGCCCA GAGGTTGATG TTTGGAGCCT GGGAGTAGTG 
    ATTTATGTTA TGGTTTGTGG ACGAGTCCCA TTTGACGACG TTTCCGTTCC TATGCTCCAT 
    TCAAAAATAA AGTCGGGTAA ACTGGAGTTT CCTTCTTACA TTTCAGAAGA TTGTTGTTCT 
    CTTATTGCAG CTATGCTGAA TGTAAACCCA CGGAAGCGCT GTTCTTTAGA ACAAGCAGCA 
    AAATTTCCCT GGTTAAAAAA AAATTCGTTT TGTTTGTATC TGCCTATTCC CTTAACCTCT 
    ATTCCCTCAA CGCCTTCAAT CCGATCTCAT GTTTTCAAAC CGCCTTTTAA TTTAAAGGTT 
    TTACAGCTGT TACATGAGCA TGGTTTAGCA AGTATTCCCG AACTAAAACA TGAACTTTAC 
    ATGGCTTACA TTGAGAGAAA AACAACTTCA TTAGTCTGTC TGTATTTATT GGGTGTCGAA 
    AGTCTCGCTC CAGCACTTCG AATTCCAACG GCTTTACCTC CTGTATATAG TAGACACCAA 
    CGGCATCATT CAGAAATTCT GGGTGCGATG GATTTGACAG AGAAAATTAC TGCTATGCAG 
    TGTCCACCTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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