ORF » Species Summary » Schizosaccharomyces pombe » efc25 cDNA ORF clone

efc25 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following efc25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the efc25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc101509 NM_001022041.2
Latest version!		 Schizosaccharomyces pombe Ras guanine nucleotide exchange factor efc25 (efc25), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    efc25 ( NM_001022041.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596123.1 Schizosaccharomyces pombe Ras guanine nucleotide exchange factor efc25 (efc25), mRNA.
    Price & Availability↑
    CloneID OSc101509
    Clone ID Related Accession (Same CDS sequence) NM_001022041.2
    Accession Version NM_001022041.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product Ras guanine nucleotide exchange factor efc25
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022041.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGAAGAC CAAATTTGGA TCGTTTACGC CTCAAATCAA GGCAGGGTTT TGAGACTTCT 
    GTTTCCAAAC CCAGTACTCC TAGTTATTCT ACGTATTCTC TTTCTCCCAC ATTTTCAGAC 
    AAGAGTGTTC TTTCTCCCTC CACTATGTCT GATTCCTACG CTCTTTCCTC TACTTACACA 
    GCCGGTTCCT CTTATCAGAA TGGCGAGTCC GATTATTTTG GTTCTTTACC CACTCCTTCC 
    TCTGATAAAT CATCGACTTC TCCCTTTCCA TATTTGAAGG GCTCCTTCGA TGATCGTTTC 
    TCTTCAACCC ATTCCCTTAC ACGACAACCT TCACCTCGTT CACCGTTAAC TCCTTTAAAA 
    GGAAATACCC GAGCTTCTCC AGAAATTCGC TATGTAAGCG ATTTCACTCC TCCCTCATCT 
    CCCTTTGAAG ACATGCAAGC GTCTGTTCAT TCTTTGCCCT TGTCTGGATG TTCGATTGGT 
    CCTGTGCGTA CTAACCATTC TTCATCTTTG TCAAATTCAT CTAACTCATT ACTACAAACC 
    GAGTCCTCCT TGCGACCCAA TTCTTCTTTC GTAAATAGTC CTTTTTTCCC CCTTCCTTCT 
    TCTGATGATC TTTTCCTTAA TGACAGAGTC ATTAATCTCT TTCTTTTTTA TGAAAAATTC 
    TCTTATTTGT TCACTCATTT ACTTTCTGCT ATAAAATCTA GAGATGCGTT GAGCATTCCA 
    TCACTCGTTC TTTCTCTCCA AGAAGAACTC TTCAATCTCT GCCAGCAGAC CGGGACTTTT 
    TATCTTCTCC AACACAATCT CTTGCAAAAC TTTGAGTTTG AAAACCCCAT TAAATTACAT 
    TTTGATAAGA TAATTCCCTA CTTTTCGAGA TTAACTGTAC TGACCTTCTC GAATCGTGCT 
    TTTATTTTTC CAGACCACAC TTTTCCCCGC TTACAGCAAA GCGCTGAGGA TTTTCTGTAC 
    CATTTACAAT TTTTCTTTAC TCTGTGTGCA AATAATAGTT TATACCTTAG TCGCTTTTGT 
    TACTACCCTT CTTTTGTTCC CAATACTCCT TTCGGGGGCA AATGGACCAA CAATGGTTTG 
    TCTGCTGTTT CATCAGCATA TCGAACACGC TTGCTTGAGC CTTGCCTACC CGAACTCGAC 
    AAGTGCGTAT GGTTTTTATT GAAAAATTGT GATGAATTTA TTGAAAACTT CTCTGATTTT 
    GCCGATGAAG AGTATGTCTT TGAAATTTGT TCCACAATTA CTAGCCACTC TGAACAAATC 
    TTCAACAAAC TTGAAAGTTG GGATATGTCG ATATACTTTG ATAAAGATTT AAGTGAGTGC 
    GAGCAAGCTA CAAACTTTGC AGTCCAATCT TACTTTGTTA CAAAGCAACG TTGTTATGAT 
    TTATTAACTG ATCTGGTTTG TTCATCTCAA GACTTGATGA TGGAGCACTC AAACGATTTT 
    TCTACAATGC CTACAATGAT TGCTTCCATT GCTGTGGCTT TCCAAACTTT ATTTGAAAAT 
    GTTTGTGATT TTCTTAAAGT ACGTGCTGCT CTCGTCGATG AGATGCAGGA ATTAGCAACA 
    AAAGAGTTTG AAAACAAGTT TTCAAATGCA AACACAGCAA AAGATGACGA ACCGGCTCGT 
    CAAACTAACA AAGGTACTAC TAGAATTTCT AGAAGTTCTG ATTTTACCGC TGTTAGCGAG 
    ATGAGCAAGG ATACCTTAAC ACTTGGCAGA AACTCTTTGC AATCAATTTT GATGCTGGAT 
    AATTTGTTAA CCAATAAAGT TGTGCAAAGC GATAACAATG TTAAAGGTGG AACTTTACCG 
    GCCCTCGTGC ATTACCTTGT CCAGAACGTT CACTTGAACA AAGATTTTCG CCATTCGTTT 
    CTTTTGACTT ATAAAACGTT CACTACCCCC CAGGAATTGT TTACGCTTCT TGTCATTCTG 
    TTTCACGAGT TACCGCCACC AGGATTAGAT GCTACAGCAT ATAGTTCTTG GGAAAAGGGA 
    GATAATTTTG TTACCAAGAA GAACGTCTGC ACCGTTATGA ATTTGTGGGT TCAAAAATAC 
    TTTTTTGAAG ATTTAAAAGC CCGTAACACT CTTTATTTGA TTTCGGAGAT GCGTACCTTT 
    TTACGTGACC ATGTTGTTCC ATCTTTTCAT ATTGGCAGTG TAATTTTATC GGAAATTGAC 
    AATCTCTGGA CTGAAGAGCC TCCAGATTCG TTAACCCAAC GCCTATTATC TTCTCCCATG 
    GCTACTTTTA TTTCTTTAAA CGTATATGCT TATACTCCTG AAGAATTTGC TTCCCAGATG 
    ACATTATTGG AGTTTGATTA TCTAAAGCAG ATTCCTTCGA GGGAATGGAT ATTTAGAAGC 
    TGGGTTAGTC GCGATTCTCG CAGCGCTGTC CGAAATTATA TAAACTTTTC GAATTGTTTC 
    ACCTATTGGA TAATCAATTG TATTTTAGAG AAAAAGAATA CCAAAGCTCG TACAGCAGTA 
    ATATCCTTTT TTATACAAAC TGCCTACAAG TGCTTGTCCC TCCAAAACTT TTCCACTCTA 
    ATGTCTATTG TTTCTGCCTT AAATTCTGCT CCTATTTATC GTTTACATGC AGCGTACAAA 
    CTTGTTAAAG CTGAAGACAT AATATGTCTG TCTGGTTTAC GTGAAATCGT GGAAACAAAG 
    AAAAATTTCA GTACTTATCG TGCTTTACTT CGCAAGGCTG AACTTCCTTG CGTACCGTTT 
    TTGGGTGTCA TATTAAGTGA CTTGACTTTT ATCGATGAAG GTAACCCCGA TGTTCTTGAT 
    TCATCTCCAC ATCTGTTAAG TTTTAATAAG CGGCATCGAC TTGCCGATGT CGTCGCAGAT 
    GTGTGCCGAT TCCAATCCTC CTCTTACGAA ATGCAATCTA ATACTGATCT TCAAAGCTAT 
    ATTTTACATC GGTGTCGCTT TGTTAACCAA GATCTGTCCT ATTTATTTGA TAAATCACTC 
    TCACTTGAAC CTCGTTCTTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_596123.1
    CDS547..3510
    Translation

    Target ORF information:

    RefSeq Version NM_001022041.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe Ras guanine nucleotide exchange factor efc25 (efc25), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001022041.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGAAGAC CAAATTTGGA TCGTTTACGC CTCAAATCAA GGCAGGGTTT TGAGACTTCT 
    GTTTCCAAAC CCAGTACTCC TAGTTATTCT ACGTATTCTC TTTCTCCCAC ATTTTCAGAC 
    AAGAGTGTTC TTTCTCCCTC CACTATGTCT GATTCCTACG CTCTTTCCTC TACTTACACA 
    GCCGGTTCCT CTTATCAGAA TGGCGAGTCC GATTATTTTG GTTCTTTACC CACTCCTTCC 
    TCTGATAAAT CATCGACTTC TCCCTTTCCA TATTTGAAGG GCTCCTTCGA TGATCGTTTC 
    TCTTCAACCC ATTCCCTTAC ACGACAACCT TCACCTCGTT CACCGTTAAC TCCTTTAAAA 
    GGAAATACCC GAGCTTCTCC AGAAATTCGC TATGTAAGCG ATTTCACTCC TCCCTCATCT 
    CCCTTTGAAG ACATGCAAGC GTCTGTTCAT TCTTTGCCCT TGTCTGGATG TTCGATTGGT 
    CCTGTGCGTA CTAACCATTC TTCATCTTTG TCAAATTCAT CTAACTCATT ACTACAAACC 
    GAGTCCTCCT TGCGACCCAA TTCTTCTTTC GTAAATAGTC CTTTTTTCCC CCTTCCTTCT 
    TCTGATGATC TTTTCCTTAA TGACAGAGTC ATTAATCTCT TTCTTTTTTA TGAAAAATTC 
    TCTTATTTGT TCACTCATTT ACTTTCTGCT ATAAAATCTA GAGATGCGTT GAGCATTCCA 
    TCACTCGTTC TTTCTCTCCA AGAAGAACTC TTCAATCTCT GCCAGCAGAC CGGGACTTTT 
    TATCTTCTCC AACACAATCT CTTGCAAAAC TTTGAGTTTG AAAACCCCAT TAAATTACAT 
    TTTGATAAGA TAATTCCCTA CTTTTCGAGA TTAACTGTAC TGACCTTCTC GAATCGTGCT 
    TTTATTTTTC CAGACCACAC TTTTCCCCGC TTACAGCAAA GCGCTGAGGA TTTTCTGTAC 
    CATTTACAAT TTTTCTTTAC TCTGTGTGCA AATAATAGTT TATACCTTAG TCGCTTTTGT 
    TACTACCCTT CTTTTGTTCC CAATACTCCT TTCGGGGGCA AATGGACCAA CAATGGTTTG 
    TCTGCTGTTT CATCAGCATA TCGAACACGC TTGCTTGAGC CTTGCCTACC CGAACTCGAC 
    AAGTGCGTAT GGTTTTTATT GAAAAATTGT GATGAATTTA TTGAAAACTT CTCTGATTTT 
    GCCGATGAAG AGTATGTCTT TGAAATTTGT TCCACAATTA CTAGCCACTC TGAACAAATC 
    TTCAACAAAC TTGAAAGTTG GGATATGTCG ATATACTTTG ATAAAGATTT AAGTGAGTGC 
    GAGCAAGCTA CAAACTTTGC AGTCCAATCT TACTTTGTTA CAAAGCAACG TTGTTATGAT 
    TTATTAACTG ATCTGGTTTG TTCATCTCAA GACTTGATGA TGGAGCACTC AAACGATTTT 
    TCTACAATGC CTACAATGAT TGCTTCCATT GCTGTGGCTT TCCAAACTTT ATTTGAAAAT 
    GTTTGTGATT TTCTTAAAGT ACGTGCTGCT CTCGTCGATG AGATGCAGGA ATTAGCAACA 
    AAAGAGTTTG AAAACAAGTT TTCAAATGCA AACACAGCAA AAGATGACGA ACCGGCTCGT 
    CAAACTAACA AAGGTACTAC TAGAATTTCT AGAAGTTCTG ATTTTACCGC TGTTAGCGAG 
    ATGAGCAAGG ATACCTTAAC ACTTGGCAGA AACTCTTTGC AATCAATTTT GATGCTGGAT 
    AATTTGTTAA CCAATAAAGT TGTGCAAAGC GATAACAATG TTAAAGGTGG AACTTTACCG 
    GCCCTCGTGC ATTACCTTGT CCAGAACGTT CACTTGAACA AAGATTTTCG CCATTCGTTT 
    CTTTTGACTT ATAAAACGTT CACTACCCCC CAGGAATTGT TTACGCTTCT TGTCATTCTG 
    TTTCACGAGT TACCGCCACC AGGATTAGAT GCTACAGCAT ATAGTTCTTG GGAAAAGGGA 
    GATAATTTTG TTACCAAGAA GAACGTCTGC ACCGTTATGA ATTTGTGGGT TCAAAAATAC 
    TTTTTTGAAG ATTTAAAAGC CCGTAACACT CTTTATTTGA TTTCGGAGAT GCGTACCTTT 
    TTACGTGACC ATGTTGTTCC ATCTTTTCAT ATTGGCAGTG TAATTTTATC GGAAATTGAC 
    AATCTCTGGA CTGAAGAGCC TCCAGATTCG TTAACCCAAC GCCTATTATC TTCTCCCATG 
    GCTACTTTTA TTTCTTTAAA CGTATATGCT TATACTCCTG AAGAATTTGC TTCCCAGATG 
    ACATTATTGG AGTTTGATTA TCTAAAGCAG ATTCCTTCGA GGGAATGGAT ATTTAGAAGC 
    TGGGTTAGTC GCGATTCTCG CAGCGCTGTC CGAAATTATA TAAACTTTTC GAATTGTTTC 
    ACCTATTGGA TAATCAATTG TATTTTAGAG AAAAAGAATA CCAAAGCTCG TACAGCAGTA 
    ATATCCTTTT TTATACAAAC TGCCTACAAG TGCTTGTCCC TCCAAAACTT TTCCACTCTA 
    ATGTCTATTG TTTCTGCCTT AAATTCTGCT CCTATTTATC GTTTACATGC AGCGTACAAA 
    CTTGTTAAAG CTGAAGACAT AATATGTCTG TCTGGTTTAC GTGAAATCGT GGAAACAAAG 
    AAAAATTTCA GTACTTATCG TGCTTTACTT CGCAAGGCTG AACTTCCTTG CGTACCGTTT 
    TTGGGTGTCA TATTAAGTGA CTTGACTTTT ATCGATGAAG GTAACCCCGA TGTTCTTGAT 
    TCATCTCCAC ATCTGTTAAG TTTTAATAAG CGGCATCGAC TTGCCGATGT CGTCGCAGAT 
    GTGTGCCGAT TCCAATCCTC CTCTTACGAA ATGCAATCTA ATACTGATCT TCAAAGCTAT 
    ATTTTACATC GGTGTCGCTT TGTTAACCAA GATCTGTCCT ATTTATTTGA TAAATCACTC 
    TCACTTGAAC CTCGTTCTTC TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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