mok12 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	mok12
Entrez Gene ID	2540243
Full Name	alpha-1,3-glucan synthase Mok12
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021514.2	NP_595619.1	alpha-1,3-glucan synthase Mok12

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mok12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mok12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101221	NM_001021514.2 Latest version!	Schizosaccharomyces pombe alpha-1,3-glucan synthase Mok12 (mok12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mok12 ( NM_001021514.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_595619.1 Schizosaccharomyces pombe alpha-1,3-glucan synthase Mok12 (mok12), mRNA.

Price & Availability↑

CloneID	OSc101221
Clone ID Related Accession (Same CDS sequence)	NM_001021514.2
Accession Version	NM_001021514.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7059bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	alpha-1,3-glucan synthase Mok12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021514.1.

ATGCTCTTCT TTAGCATACC TACAATTTTG TTAATATTTT TCCTATTTAT CCAAATTATA 
ACAGCAGCCT TCCTTACTGA TGAAAATGAA CCTTGGAATC TAAATCGTAA TTGGACAGCT 
CAAAAAATTT TGGATTATAG TGCCGAATGG AATTCTCATG AGTATTTTCC TTCTCCATCT 
AACTGGAGAG CGCTACCTTT TTACACCATT ATCCTGGATA AATGGACCAA CGGAGTTCCC 
GAAAATGATG TTGCTGAAGA TACTGTCTTT GAATCTGACC CTTATGAAGT TACATTTCGG 
GCAGGTGGTG ATATTGTTGG ACTTGTAACT CCAAGGTCCC TAGATTATCT TGAAAGCATG 
GGCATTAAAG CTGTTTATAT AGCTGGTACA CCGTTCCAGA ACTTGCCTTG GTACCCAGAT 
GGCTACTCTC CCCTTGATTT TACTTTGCTC GACAAGCATA CTGGAACTCT CAATCAATGG 
CATGAAGCAA TAATGAAATT GCATGAGAGA GGGTTTTATG TAGTAGTTGA TTTTACTATA 
TCTACTCTTT CTGAGCTTAG CTATTTCGTG AATTCTTCAA TGTCGTTCGC GAATACTTCT 
GCTCCTTTTA GCACCAAAGG ATATAAAATG AAGTATAAGC ACCCAGAATA TCATTACACA 
GATTTTCAAT TGTCTAACGG GTCTTCGTAT TCTTGTAATG CCCCTACCTT TTGGGATGTT 
ACGGGTCTTC CAATCAATAA TACCGAAGAC CTTAATTCGA TTTCTGAAGT TATGTGTCTA 
AGTGGTGATT TCGACCACTA TGGAGATGTC GAAGCTTTTG GTAATCATCC TCCTTGGTGG 
CGCCAACTGT CAAATTTTGC TTCAGTGCAA GATAGATTGA GAGATTGGGA CCCAATTGTT 
GCTAAAAAGC TTAAACATTT AGGATGTTTA GCTGTAAAAA TGCTCGATAT TGATGGAATC 
CGTGTTGATA AGGCCACTCA AATTACAGCT GATTTCCTTG GAGATTGGAG CGCTTATATT 
CGCCAATGTG CTCGTGAAAT AGGAAAAGAG AATTTTTTTA TTCCCGGTGA AGTTACTAGT 
GGCGCTGATT TTGGCTCAAT ATATGTCGGG CGTGGTCGTC AAGCAGATCA GCGCCCCAAT 
AACCGAGAAA TAGCTTTGCA GACTGGTTAT AATGAATCAA AATACTTCTT AAGGAAAGAA 
TCCGATTCAG CTCTTGACTC CGTTTCATTT CACTACTCAG TATATCGTAC TCTAACATTA 
CTGCTTGGTC TGCAGGGAGA ACTATTTGCT GCATTCGATC TTAATCGTCA TGACTTTGCT 
GCCATGTGGA ACCAAATGCT GATTCAAGAT GATATGATAA ACGCTAATAC CAAAAAATTT 
GATCCTAGAC ATCTTTATGG TTTGACAAAT CAAGATATTT TCCGTTGGCC CTCCATTAAA 
GATGGAAGAT TCAAACAGCT TTTGGGGCTT TTTGTAGTAC ACCTACTTAT GCCCGGTATT 
CCTCTAATTT ATTATGGGGA AGAGCAAAAT CTCAAATTAC TTGATAACCA AGCAGCAAAT 
TATATATTCG GTCGCCAGCC TATTACTTCT TCGATTGGAT GGCAAAAGCA TGGCTGTTAT 
CAAATTGGCA CTACTCAGTA TACTGACTTA GACTTTGGAC CTGCAAGTGA GGCATGTAGG 
GACGATTGGA ACAGTTTGGA CCATCTTGAT CCCACTTCTC CTACTAAGCA TTATATTGCC 
AGAATGAACG AGATTCGCTC TTATTTCCCT CAAGTTAGAG ACGGATGGGA CTTGAAATTG 
ATAGGTAAAT GGACGCATGA AGGTACTTTC CCTGGTAATG AATTATATGG TGAATCAAAT 
CCAACAACTT GGGGGTTATG GTCAATGATA AGAGGACCTC TTGCACCATA TCAAAAATTT 
TCTGATCAAA ATGATTACAT TTGGCTTATT TTCACAAATG AGAATACTAC CAAGACATAC 
GACATGGATT GTATGAGACA AGTTGATAAC AAATTTCCTC CGTATCCGGC GCTTTTAGGC 
CCTTATTCTT CTGGATCTAC AGTAAAGAAT CTTCTTTACC CTTACGAGGA AATCGACCTT 
AGTACCTCAA CTATTGATTC TCCAGATATT GGAAATATCG GATGCATCCC TCACTTAACT 
ATTGACGCAT ACGGATCTAA AATATTTGTT AAAAAAGAGG ACTGGATTAG ACCTTCTTTA 
TATTTGACCA AATTCTTACC GGGCCATGAT TCTCGTATAT ACTCAGCAAC TGAAGTTACA 
TCTTTCAAAA TTAGTCTTGG TTTTTCAGAA GAAGTCGATT GTAAAGATCT ATTTAAACGT 
ATCGGCTTTA ATTCGCGTAC ATTAAATAGT TCATTTGCTC CAAAAATTCT AGAAGATAGC 
ATTTCTTGCG GATATTTGGA TCAACCTGCA CCTCAAGAAT ACACGAATGC TCCAGTTACA 
AAGTGGTTTT TCAATGCTAC TCTTGATTCC GTCCCGAACG GGATGCACGA GTTACTTTTA 
AATGAAGTTA AAAGTACATC CAATCAAACA ATGCAATCAA AAATTGCTAG ACTCATATTT 
CGAGTAGGAA ATGAGGAAAA TCCTTTGGTC TATCCAAATA ATGCTACCTT CAGCCCTTCT 
CTTCTTTATA AAGCTAGTAA CGGAGACTTA TACGTAAATC ACACTGGTGC TGGCGCTGAT 
AAGTATCGGT TCTCATTGAA CTATGGTGGA ACGTACTCAA AATGGAAAAC TGTATCAACT 
CCCTCCGAGA AGCTGCGAAA ACCGACCTGG AACGGCACTA ATTTGCAAAA ATGGGATGGG 
GACCATCTTA TAGTTCAATA CTGGTCTAGT ATCGCTCTTT CTACTGCTCA CGTTCAGCAT 
GGTGATACGT TAAATTATTC TCGTCAATTT CCTAATCTTT TCGTTCAAGG TGCGTTTAAC 
AGATTTGGCT ACGATGGTTC AATGCCAAGT AAGATGTCAT ATAATTTAGA GAATCGGACT 
TGGAGTTATG ATTTAATTTC AACTTGGCCT GCTGAACTTG TTCTAAACGT TTGGGGCATG 
AATCCAGACA ATAATGCCGA TCAAGGATGG GTTTACGGTG ATTTAGATAA TGATACTATA 
ATTGATAGAG TTCCCCCAGG AAGTAGTCGT ATATCTAATT TTATTCGTTT CCTTGATCCG 
CCCCCGAAAC CATACCTTTC TTATAAAATG TATCTGAACG ATTTTACGAG GCAGTTACAT 
TATATTCCAA AGGGTTCTTG GACAGTTCAA ATCGTTGCCA TTGTTCTACT GATTCTTCTT 
CCACCCCTAT TTGGTATTTT TTCGGTAGCT TTGTACAGCG GCGCATTTTC CAGAGTTACC 
ATTTTTAACG GCTCCCATAA TAGGGGCATA AAAGTTGCGA AACAAAAGAT CAAGAGCATA 
ATGTCTCGGA TATTTCCATT TAGTGTTCAT TTACCTCTCG ACAATAGTTC AGAATTGCTC 
AAACAGCTTC CAGAGTCTGA AAAACGATTA AATGTTCTTG TAGCCACCTT AGAATATGAT 
GTACCCGATC TGGGCATTCG AGTTAAAATA GGCGGTTTGG GAGTAATGGC ACAACTAATG 
GGACAGCACA TGGAGTTTCA AGATATGGTA TGGGTCGTTC CAATAATAAG TGGTGTCGAA 
TATCCATTCG ATAAACTTTG TACAGAACCC AAGATTGCTG TTAAAATTGG TGATGATGAA 
TTTGTTGTTA ACTGCTATTC ATATAAATCG GGAAATATAA CATACGTCTT TCTGCAATCA 
GAAGTTTTTT ACAAGCAGTC TAGTAAAGAA CCATATCCAT TAAAAATGGA TGACTTGGCT 
TCGGCAATTT TTTATTCTGT TTGGAACCAA TGCATTGCTG AAGTTTGGAA AAGGTTTCCG 
TTAGATATAT ATCATGTGAA TGATTATCAT GGAGCACTTG CTCCTCTCTA TTTATTACCA 
GAAGTTATAC CTGTAGCTGT GTCTTTACAC AATGCCGAGT TCCAAGGATT ATGGCCTCTC 
CGTACGTCGG CTGAGCTTGA ATGTGTTTGC TCAATTTTTA ACATTGAAAA GGACATTTGT 
ACCAAGTATG TACAATTTGG ACATGTTTTT AATTTGTTGC ATTCTATTAT CTCTTATGTT 
CGGAAACATC AAGGAGGGTA CGGCGTTGTA GCGGTCTCAG ATAAATACAG TAAACAAACT 
CTGTCAAGGT ATCCTATTTT TTGGAGTCTC GTTCATATTA GTGGATTGCC AAATCCTGAC 
CCCAGTGATC TTAAATTGCT TAATCATCTT ACAGATGATC CCGTTGATGT TGACTTTGTT 
TTGGAAGCGA AAAGAAAATT GCTTAAAAAA CAGACACAAG AATGGGCGAA TTTGGATGTT 
GATCCTTCAG CACAATTACT AGTATTTGTT GGTAGGTGGT CACATCAAAA AGGCATAGAT 
TTGATTGCTG ATCTTGCTCC TAAACTTTTG ACGGAACACA ATGTGCAGTT GATTACGATT 
GGTCCTGTGA TTGATTTGCA TGGCCAATTC GCAGCAGAAA AACTTGAGCA AATAGCCAAA 
CGTTTTCCGA CTCGAGTTCT ATGCAAGCCA GTATTTACGG CGGTTCCTCC TTTTCTGTTC 
GCTGGAACTG ATTTTGCATT AATTCCTTCT AGAGATGAGC CATTTGGTTT GGTTGCTGTC 
GAGTTTGGAA GAAAGGGTGT TCTGTGCATT GGTTCAAGAA CAGGAGGGCT TGGTCATATG 
CCTGGTTGGT GGTTTCAAAT GGCATCACCT AACACAGGGC ATTTGCTTAC TCAATTCGAA 
AATGCAATAA CGAAAGCTCT CCACAGCAAC GGTGAGCTGA GGGCTCGACT TCGAGTTGAA 
GCTTTACGCC AACGATTTCC TGTTTGCATT TGGAAACAGA AATCGGAAAA TTTGTTGAAA 
TCGTGTATAT ATGTGCATGA AATGGAAACA CTCAAGCATG CCAGCCCAAC CTTTAAAGCG 
TATCAATTCG TTGTACGCAT CTGTCATTAC TTAACTTCTA AGGTTAAATC AATAAATAAG 
GATCAAGTTT TTAATGCTTT CTCACCCGAT GTTTCAGAAC GCCCTCCATT ATTTGAAGTA 
GCCTCTTGTT CAAATGAAAC TGACTCTGTG GAGAAGCTAA CACCTGAGTT ATCCGTTTCG 
CCAGAGTCGG ATATGCATTT TAAAGATGGG AATTCAAGTA AATTAGAAAT TGTTGAAAGC 
AAGGAAATTT ACGCGTCTGA CAGTGATATA AGTAATAGTA CATCCGAGGA TATCAATAAA 
GATGAATGTG TCTCTAAAGA CATAGAAGTC GATAATTTTG CACTCAACTT GCAGTCTCAG 
TCTTACGAAG GAGATTCTAA TGATTTCGGA ATTCGCGAGG TTCCACTCTC TGATGCAAAC 
CAATCGTCAC AAGCCGATTC AACTTCAATC GATAGATATG GACCCTATAG CTCACAAAAA 
GTTAACTTTT CTAAATATAA AGATTTTGTT GAATCGCGAC CTACATTTTT TCAAAGTTCT 
GTTACATTCA CGGATGCTGA TGGTTCTACA AGGAAAACTT TCTCAAAGAG GCTTGAAAAT 
TTGACTACGT CTAACACCCT TAAATCTTTA TCAGTTGACC ATTTTATTCG TAAGCATGAG 
AGAAAGTACT TCAATGGACT AAGGAAGCAA GAAATTGATG TGAAATTAAA GTCTCGCTCA 
GATAAAGAAA AGAGTTGCAC AGTCTCGGAG TCTTACAAGC AAATTGATTG TGATACATAT 
GAAGCTACTC GGTTACAAAA ATTACTTCTT CACAGTATTG GTGGATGGCC TCTATATACG 
ATTGTATTGG CAATTGGGCA AATTCTTGGA GCATCTTCTT ATCAATTAAC TTTACTTTCT 
GGAGAATCTG CGCAATCTAC AGTGTCCATG TACATTCTGC TCTCTATTTT CTCATTCTTC 
TCTCTGTTTT GGTGGTTCTT ATCCCGAGTG GTTCAAGCGA GATACATTTT ATCTCTCCCT 
TTCTTCTTTT TTGGCATATC TTTCATTTTA GTTGCGATCA CTCACTTTTT CCAGAAAACT 
ACTGCTTGTT CGGTAATTCA ACACATAGCG GCTTACGTTT ATGCCATTTC AAGTTCTACT 
GGATCGCTGT ATTTTGCATG GAATTTTGGT GCCGAAGGAG GTATCGCAAC TCATCATTGG 
ATTTTGAGGG CCTGTCTTGT ACATGGGATT CAACAGATTT GGTCTGCAAT CCTCTGGTCA 
TGGGGAGATC TATTATCGAA GAAAGATTTG ACTCAAAATG TGGGGCCTGG CATTTTTGCA 
GGGGGACTTA TCGCCTCTTT CATTTGCTTC GGGCTTTCCT ACGTAACATT CGCAGGTTTA 
CCTGCTTTTT ATCGCCAAGC TCCTTCTATC ATTCCAGCGT TTTATCGTTC ATTAGGAAAA 
CGAAATATTG TAATTTGGTT TTTTATCTCT CAAATTTTAA TCAATTACTG GCTTGCTGTC 
CCTTATGGCC AGGCTTGGAG GTTTTTCTGG AATACTAGCA ACACTCCTTT GTGGAGCATA 
ATCATTCTTT TACTCATTTT CTTCATCGTG GTTTGGGCCG TTCTCTTATC AGTTATAAAG 
ATTTTGTCTC TTAATAACGT TTGGTTTCCA GTGATATTTG GATTGGGCCT CATTTGTCCA 
CGCTGGTGCT TAGAGTTTTG GTCATCCTCG GGTCTCGGAA TTAATTTACC ATGGGCTGGT 
AAGGCTTCTG CCTTGCTGAC GAAGTCAGTA TGGCTTTTGC TAGCTCTATG GGACGGGATA 
CAGGGAGTCG GTGTCGGTGT AATGTTACTG CAAACCCTGG CGAGAGATCA TGTTGCTTTC 
ACGTTGATGC TAGCGCAAGT TATAAGTTGT ATTACAATTA TGATCGCAAA ACCTTCTCTG 
CCTGTAAGTG ATCGAGTATT CCCAAATCTT GGTGCATGGA ATCCTAGCGA AGGACCGGGA 
CCTTGTGCAT CACCTTGCTT TTATATTGCA CTAATATGTC AATTTGTAGC TGTTGGAGGA 
TTGCTCTATC ACTATCGTAA AAGTCAGCTT GCTCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_595619.1
CDS	955..8013
Translation	MLFFSIPTILLIFFLFIQIITAAFLTDENEPWNLNRNWTAQKILDYSAEWNSHEYFPSPSNWRALPFYTIILDKWTNGVPENDVAEDTVFESDPYEVTFRAGGDIVGLVTPRSLDYLESMGIKAVYIAGTPFQNLPWYPDGYSPLDFTLLDKHTGTLNQWHEAIMKLHERGFYVVVDFTISTLSELSYFVNSSMSFANTSAPFSTKGYKMKYKHPEYHYTDFQLSNGSSYSCNAPTFWDVTGLPINNTEDLNSISEVMCLSGDFDHYGDVEAFGNHPPWWRQLSNFASVQDRLRDWDPIVAKKLKHLGCLAVKMLDIDGIRVDKATQITADFLGDWSAYIRQCAREIGKENFFIPGEVTSGADFGSIYVGRGRQADQRPNNREIALQTGYNESKYFLRKESDSALDSVSFHYSVYRTLTLLLGLQGELFAAFDLNRHDFAAMWNQMLIQDDMINANTKKFDPRHLYGLTNQDIFRWPSIKDGRFKQLLGLFVVHLLMPGIPLIYYGEEQNLKLLDNQAANYIFGRQPITSSIGWQKHGCYQIGTTQYTDLDFGPASEACRDDWNSLDHLDPTSPTKHYIARMNEIRSYFPQVRDGWDLKLIGKWTHEGTFPGNELYGESNPTTWGLWSMIRGPLAPYQKFSDQNDYIWLIFTNENTTKTYDMDCMRQVDNKFPPYPALLGPYSSGSTVKNLLYPYEEIDLSTSTIDSPDIGNIGCIPHLTIDAYGSKIFVKKEDWIRPSLYLTKFLPGHDSRIYSATEVTSFKISLGFSEEVDCKDLFKRIGFNSRTLNSSFAPKILEDSISCGYLDQPAPQEYTNAPVTKWFFNATLDSVPNGMHELLLNEVKSTSNQTMQSKIARLIFRVGNEENPLVYPNNATFSPSLLYKASNGDLYVNHTGAGADKYRFSLNYGGTYSKWKTVSTPSEKLRKPTWNGTNLQKWDGDHLIVQYWSSIALSTAHVQHGDTLNYSRQFPNLFVQGAFNRFGYDGSMPSKMSYNLENRTWSYDLISTWPAELVLNVWGMNPDNNADQGWVYGDLDNDTIIDRVPPGSSRISNFIRFLDPPPKPYLSYKMYLNDFTRQLHYIPKGSWTVQIVAIVLLILLPPLFGIFSVALYSGAFSRVTIFNGSHNRGIKVAKQKIKSIMSRIFPFSVHLPLDNSSELLKQLPESEKRLNVLVATLEYDVPDLGIRVKIGGLGVMAQLMGQHMEFQDMVWVVPIISGVEYPFDKLCTEPKIAVKIGDDEFVVNCYSYKSGNITYVFLQSEVFYKQSSKEPYPLKMDDLASAIFYSVWNQCIAEVWKRFPLDIYHVNDYHGALAPLYLLPEVIPVAVSLHNAEFQGLWPLRTSAELECVCSIFNIEKDICTKYVQFGHVFNLLHSIISYVRKHQGGYGVVAVSDKYSKQTLSRYPIFWSLVHISGLPNPDPSDLKLLNHLTDDPVDVDFVLEAKRKLLKKQTQEWANLDVDPSAQLLVFVGRWSHQKGIDLIADLAPKLLTEHNVQLITIGPVIDLHGQFAAEKLEQIAKRFPTRVLCKPVFTAVPPFLFAGTDFALIPSRDEPFGLVAVEFGRKGVLCIGSRTGGLGHMPGWWFQMASPNTGHLLTQFENAITKALHSNGELRARLRVEALRQRFPVCIWKQKSENLLKSCIYVHEMETLKHASPTFKAYQFVVRICHYLTSKVKSINKDQVFNAFSPDVSERPPLFEVASCSNETDSVEKLTPELSVSPESDMHFKDGNSSKLEIVESKEIYASDSDISNSTSEDINKDECVSKDIEVDNFALNLQSQSYEGDSNDFGIREVPLSDANQSSQADSTSIDRYGPYSSQKVNFSKYKDFVESRPTFFQSSVTFTDADGSTRKTFSKRLENLTTSNTLKSLSVDHFIRKHERKYFNGLRKQEIDVKLKSRSDKEKSCTVSESYKQIDCDTYEATRLQKLLLHSIGGWPLYTIVLAIGQILGASSYQLTLLSGESAQSTVSMYILLSIFSFFSLFWWFLSRVVQARYILSLPFFFFGISFILVAITHFFQKTTACSVIQHIAAYVYAISSSTGSLYFAWNFGAEGGIATHHWILRACLVHGIQQIWSAILWSWGDLLSKKDLTQNVGPGIFAGGLIASFICFGLSYVTFAGLPAFYRQAPSIIPAFYRSLGKRNIVIWFFISQILINYWLAVPYGQAWRFFWNTSNTPLWSIIILLLIFFIVVWAVLLSVIKILSLNNVWFPVIFGLGLICPRWCLEFWSSSGLGINLPWAGKASALLTKSVWLLLALWDGIQGVGVGVMLLQTLARDHVAFTLMLAQVISCITIMIAKPSLPVSDRVFPNLGAWNPSEGPGPCASPCFYIALICQFVAVGGLLYHYRKSQLAL

Target ORF information:

RefSeq Version	NM_001021514.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe alpha-1,3-glucan synthase Mok12 (mok12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021514.2

ORF Insert Sequence:

ATGCTCTTCT TTAGCATACC TACAATTTTG TTAATATTTT TCCTATTTAT CCAAATTATA 
ACAGCAGCCT TCCTTACTGA TGAAAATGAA CCTTGGAATC TAAATCGTAA TTGGACAGCT 
CAAAAAATTT TGGATTATAG TGCCGAATGG AATTCTCATG AGTATTTTCC TTCTCCATCT 
AACTGGAGAG CGCTACCTTT TTACACCATT ATCCTGGATA AATGGACCAA CGGAGTTCCC 
GAAAATGATG TTGCTGAAGA TACTGTCTTT GAATCTGACC CTTATGAAGT TACATTTCGG 
GCAGGTGGTG ATATTGTTGG ACTTGTAACT CCAAGGTCCC TAGATTATCT TGAAAGCATG 
GGCATTAAAG CTGTTTATAT AGCTGGTACA CCGTTCCAGA ACTTGCCTTG GTACCCAGAT 
GGCTACTCTC CCCTTGATTT TACTTTGCTC GACAAGCATA CTGGAACTCT CAATCAATGG 
CATGAAGCAA TAATGAAATT GCATGAGAGA GGGTTTTATG TAGTAGTTGA TTTTACTATA 
TCTACTCTTT CTGAGCTTAG CTATTTCGTG AATTCTTCAA TGTCGTTCGC GAATACTTCT 
GCTCCTTTTA GCACCAAAGG ATATAAAATG AAGTATAAGC ACCCAGAATA TCATTACACA 
GATTTTCAAT TGTCTAACGG GTCTTCGTAT TCTTGTAATG CCCCTACCTT TTGGGATGTT 
ACGGGTCTTC CAATCAATAA TACCGAAGAC CTTAATTCGA TTTCTGAAGT TATGTGTCTA 
AGTGGTGATT TCGACCACTA TGGAGATGTC GAAGCTTTTG GTAATCATCC TCCTTGGTGG 
CGCCAACTGT CAAATTTTGC TTCAGTGCAA GATAGATTGA GAGATTGGGA CCCAATTGTT 
GCTAAAAAGC TTAAACATTT AGGATGTTTA GCTGTAAAAA TGCTCGATAT TGATGGAATC 
CGTGTTGATA AGGCCACTCA AATTACAGCT GATTTCCTTG GAGATTGGAG CGCTTATATT 
CGCCAATGTG CTCGTGAAAT AGGAAAAGAG AATTTTTTTA TTCCCGGTGA AGTTACTAGT 
GGCGCTGATT TTGGCTCAAT ATATGTCGGG CGTGGTCGTC AAGCAGATCA GCGCCCCAAT 
AACCGAGAAA TAGCTTTGCA GACTGGTTAT AATGAATCAA AATACTTCTT AAGGAAAGAA 
TCCGATTCAG CTCTTGACTC CGTTTCATTT CACTACTCAG TATATCGTAC TCTAACATTA 
CTGCTTGGTC TGCAGGGAGA ACTATTTGCT GCATTCGATC TTAATCGTCA TGACTTTGCT 
GCCATGTGGA ACCAAATGCT GATTCAAGAT GATATGATAA ACGCTAATAC CAAAAAATTT 
GATCCTAGAC ATCTTTATGG TTTGACAAAT CAAGATATTT TCCGTTGGCC CTCCATTAAA 
GATGGAAGAT TCAAACAGCT TTTGGGGCTT TTTGTAGTAC ACCTACTTAT GCCCGGTATT 
CCTCTAATTT ATTATGGGGA AGAGCAAAAT CTCAAATTAC TTGATAACCA AGCAGCAAAT 
TATATATTCG GTCGCCAGCC TATTACTTCT TCGATTGGAT GGCAAAAGCA TGGCTGTTAT 
CAAATTGGCA CTACTCAGTA TACTGACTTA GACTTTGGAC CTGCAAGTGA GGCATGTAGG 
GACGATTGGA ACAGTTTGGA CCATCTTGAT CCCACTTCTC CTACTAAGCA TTATATTGCC 
AGAATGAACG AGATTCGCTC TTATTTCCCT CAAGTTAGAG ACGGATGGGA CTTGAAATTG 
ATAGGTAAAT GGACGCATGA AGGTACTTTC CCTGGTAATG AATTATATGG TGAATCAAAT 
CCAACAACTT GGGGGTTATG GTCAATGATA AGAGGACCTC TTGCACCATA TCAAAAATTT 
TCTGATCAAA ATGATTACAT TTGGCTTATT TTCACAAATG AGAATACTAC CAAGACATAC 
GACATGGATT GTATGAGACA AGTTGATAAC AAATTTCCTC CGTATCCGGC GCTTTTAGGC 
CCTTATTCTT CTGGATCTAC AGTAAAGAAT CTTCTTTACC CTTACGAGGA AATCGACCTT 
AGTACCTCAA CTATTGATTC TCCAGATATT GGAAATATCG GATGCATCCC TCACTTAACT 
ATTGACGCAT ACGGATCTAA AATATTTGTT AAAAAAGAGG ACTGGATTAG ACCTTCTTTA 
TATTTGACCA AATTCTTACC GGGCCATGAT TCTCGTATAT ACTCAGCAAC TGAAGTTACA 
TCTTTCAAAA TTAGTCTTGG TTTTTCAGAA GAAGTCGATT GTAAAGATCT ATTTAAACGT 
ATCGGCTTTA ATTCGCGTAC ATTAAATAGT TCATTTGCTC CAAAAATTCT AGAAGATAGC 
ATTTCTTGCG GATATTTGGA TCAACCTGCA CCTCAAGAAT ACACGAATGC TCCAGTTACA 
AAGTGGTTTT TCAATGCTAC TCTTGATTCC GTCCCGAACG GGATGCACGA GTTACTTTTA 
AATGAAGTTA AAAGTACATC CAATCAAACA ATGCAATCAA AAATTGCTAG ACTCATATTT 
CGAGTAGGAA ATGAGGAAAA TCCTTTGGTC TATCCAAATA ATGCTACCTT CAGCCCTTCT 
CTTCTTTATA AAGCTAGTAA CGGAGACTTA TACGTAAATC ACACTGGTGC TGGCGCTGAT 
AAGTATCGGT TCTCATTGAA CTATGGTGGA ACGTACTCAA AATGGAAAAC TGTATCAACT 
CCCTCCGAGA AGCTGCGAAA ACCGACCTGG AACGGCACTA ATTTGCAAAA ATGGGATGGG 
GACCATCTTA TAGTTCAATA CTGGTCTAGT ATCGCTCTTT CTACTGCTCA CGTTCAGCAT 
GGTGATACGT TAAATTATTC TCGTCAATTT CCTAATCTTT TCGTTCAAGG TGCGTTTAAC 
AGATTTGGCT ACGATGGTTC AATGCCAAGT AAGATGTCAT ATAATTTAGA GAATCGGACT 
TGGAGTTATG ATTTAATTTC AACTTGGCCT GCTGAACTTG TTCTAAACGT TTGGGGCATG 
AATCCAGACA ATAATGCCGA TCAAGGATGG GTTTACGGTG ATTTAGATAA TGATACTATA 
ATTGATAGAG TTCCCCCAGG AAGTAGTCGT ATATCTAATT TTATTCGTTT CCTTGATCCG 
CCCCCGAAAC CATACCTTTC TTATAAAATG TATCTGAACG ATTTTACGAG GCAGTTACAT 
TATATTCCAA AGGGTTCTTG GACAGTTCAA ATCGTTGCCA TTGTTCTACT GATTCTTCTT 
CCACCCCTAT TTGGTATTTT TTCGGTAGCT TTGTACAGCG GCGCATTTTC CAGAGTTACC 
ATTTTTAACG GCTCCCATAA TAGGGGCATA AAAGTTGCGA AACAAAAGAT CAAGAGCATA 
ATGTCTCGGA TATTTCCATT TAGTGTTCAT TTACCTCTCG ACAATAGTTC AGAATTGCTC 
AAACAGCTTC CAGAGTCTGA AAAACGATTA AATGTTCTTG TAGCCACCTT AGAATATGAT 
GTACCCGATC TGGGCATTCG AGTTAAAATA GGCGGTTTGG GAGTAATGGC ACAACTAATG 
GGACAGCACA TGGAGTTTCA AGATATGGTA TGGGTCGTTC CAATAATAAG TGGTGTCGAA 
TATCCATTCG ATAAACTTTG TACAGAACCC AAGATTGCTG TTAAAATTGG TGATGATGAA 
TTTGTTGTTA ACTGCTATTC ATATAAATCG GGAAATATAA CATACGTCTT TCTGCAATCA 
GAAGTTTTTT ACAAGCAGTC TAGTAAAGAA CCATATCCAT TAAAAATGGA TGACTTGGCT 
TCGGCAATTT TTTATTCTGT TTGGAACCAA TGCATTGCTG AAGTTTGGAA AAGGTTTCCG 
TTAGATATAT ATCATGTGAA TGATTATCAT GGAGCACTTG CTCCTCTCTA TTTATTACCA 
GAAGTTATAC CTGTAGCTGT GTCTTTACAC AATGCCGAGT TCCAAGGATT ATGGCCTCTC 
CGTACGTCGG CTGAGCTTGA ATGTGTTTGC TCAATTTTTA ACATTGAAAA GGACATTTGT 
ACCAAGTATG TACAATTTGG ACATGTTTTT AATTTGTTGC ATTCTATTAT CTCTTATGTT 
CGGAAACATC AAGGAGGGTA CGGCGTTGTA GCGGTCTCAG ATAAATACAG TAAACAAACT 
CTGTCAAGGT ATCCTATTTT TTGGAGTCTC GTTCATATTA GTGGATTGCC AAATCCTGAC 
CCCAGTGATC TTAAATTGCT TAATCATCTT ACAGATGATC CCGTTGATGT TGACTTTGTT 
TTGGAAGCGA AAAGAAAATT GCTTAAAAAA CAGACACAAG AATGGGCGAA TTTGGATGTT 
GATCCTTCAG CACAATTACT AGTATTTGTT GGTAGGTGGT CACATCAAAA AGGCATAGAT 
TTGATTGCTG ATCTTGCTCC TAAACTTTTG ACGGAACACA ATGTGCAGTT GATTACGATT 
GGTCCTGTGA TTGATTTGCA TGGCCAATTC GCAGCAGAAA AACTTGAGCA AATAGCCAAA 
CGTTTTCCGA CTCGAGTTCT ATGCAAGCCA GTATTTACGG CGGTTCCTCC TTTTCTGTTC 
GCTGGAACTG ATTTTGCATT AATTCCTTCT AGAGATGAGC CATTTGGTTT GGTTGCTGTC 
GAGTTTGGAA GAAAGGGTGT TCTGTGCATT GGTTCAAGAA CAGGAGGGCT TGGTCATATG 
CCTGGTTGGT GGTTTCAAAT GGCATCACCT AACACAGGGC ATTTGCTTAC TCAATTCGAA 
AATGCAATAA CGAAAGCTCT CCACAGCAAC GGTGAGCTGA GGGCTCGACT TCGAGTTGAA 
GCTTTACGCC AACGATTTCC TGTTTGCATT TGGAAACAGA AATCGGAAAA TTTGTTGAAA 
TCGTGTATAT ATGTGCATGA AATGGAAACA CTCAAGCATG CCAGCCCAAC CTTTAAAGCG 
TATCAATTCG TTGTACGCAT CTGTCATTAC TTAACTTCTA AGGTTAAATC AATAAATAAG 
GATCAAGTTT TTAATGCTTT CTCACCCGAT GTTTCAGAAC GCCCTCCATT ATTTGAAGTA 
GCCTCTTGTT CAAATGAAAC TGACTCTGTG GAGAAGCTAA CACCTGAGTT ATCCGTTTCG 
CCAGAGTCGG ATATGCATTT TAAAGATGGG AATTCAAGTA AATTAGAAAT TGTTGAAAGC 
AAGGAAATTT ACGCGTCTGA CAGTGATATA AGTAATAGTA CATCCGAGGA TATCAATAAA 
GATGAATGTG TCTCTAAAGA CATAGAAGTC GATAATTTTG CACTCAACTT GCAGTCTCAG 
TCTTACGAAG GAGATTCTAA TGATTTCGGA ATTCGCGAGG TTCCACTCTC TGATGCAAAC 
CAATCGTCAC AAGCCGATTC AACTTCAATC GATAGATATG GACCCTATAG CTCACAAAAA 
GTTAACTTTT CTAAATATAA AGATTTTGTT GAATCGCGAC CTACATTTTT TCAAAGTTCT 
GTTACATTCA CGGATGCTGA TGGTTCTACA AGGAAAACTT TCTCAAAGAG GCTTGAAAAT 
TTGACTACGT CTAACACCCT TAAATCTTTA TCAGTTGACC ATTTTATTCG TAAGCATGAG 
AGAAAGTACT TCAATGGACT AAGGAAGCAA GAAATTGATG TGAAATTAAA GTCTCGCTCA 
GATAAAGAAA AGAGTTGCAC AGTCTCGGAG TCTTACAAGC AAATTGATTG TGATACATAT 
GAAGCTACTC GGTTACAAAA ATTACTTCTT CACAGTATTG GTGGATGGCC TCTATATACG 
ATTGTATTGG CAATTGGGCA AATTCTTGGA GCATCTTCTT ATCAATTAAC TTTACTTTCT 
GGAGAATCTG CGCAATCTAC AGTGTCCATG TACATTCTGC TCTCTATTTT CTCATTCTTC 
TCTCTGTTTT GGTGGTTCTT ATCCCGAGTG GTTCAAGCGA GATACATTTT ATCTCTCCCT 
TTCTTCTTTT TTGGCATATC TTTCATTTTA GTTGCGATCA CTCACTTTTT CCAGAAAACT 
ACTGCTTGTT CGGTAATTCA ACACATAGCG GCTTACGTTT ATGCCATTTC AAGTTCTACT 
GGATCGCTGT ATTTTGCATG GAATTTTGGT GCCGAAGGAG GTATCGCAAC TCATCATTGG 
ATTTTGAGGG CCTGTCTTGT ACATGGGATT CAACAGATTT GGTCTGCAAT CCTCTGGTCA 
TGGGGAGATC TATTATCGAA GAAAGATTTG ACTCAAAATG TGGGGCCTGG CATTTTTGCA 
GGGGGACTTA TCGCCTCTTT CATTTGCTTC GGGCTTTCCT ACGTAACATT CGCAGGTTTA 
CCTGCTTTTT ATCGCCAAGC TCCTTCTATC ATTCCAGCGT TTTATCGTTC ATTAGGAAAA 
CGAAATATTG TAATTTGGTT TTTTATCTCT CAAATTTTAA TCAATTACTG GCTTGCTGTC 
CCTTATGGCC AGGCTTGGAG GTTTTTCTGG AATACTAGCA ACACTCCTTT GTGGAGCATA 
ATCATTCTTT TACTCATTTT CTTCATCGTG GTTTGGGCCG TTCTCTTATC AGTTATAAAG 
ATTTTGTCTC TTAATAACGT TTGGTTTCCA GTGATATTTG GATTGGGCCT CATTTGTCCA 
CGCTGGTGCT TAGAGTTTTG GTCATCCTCG GGTCTCGGAA TTAATTTACC ATGGGCTGGT 
AAGGCTTCTG CCTTGCTGAC GAAGTCAGTA TGGCTTTTGC TAGCTCTATG GGACGGGATA 
CAGGGAGTCG GTGTCGGTGT AATGTTACTG CAAACCCTGG CGAGAGATCA TGTTGCTTTC 
ACGTTGATGC TAGCGCAAGT TATAAGTTGT ATTACAATTA TGATCGCAAA ACCTTCTCTG 
CCTGTAAGTG ATCGAGTATT CCCAAATCTT GGTGCATGGA ATCCTAGCGA AGGACCGGGA 
CCTTGTGCAT CACCTTGCTT TTATATTGCA CTAATATGTC AATTTGTAGC TGTTGGAGGA 
TTGCTCTATC ACTATCGTAA AAGTCAGCTT GCTCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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mok12 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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