mug81 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	mug81
Entrez Gene ID	2539673
Full Name	ATP-dependent RNA helicase Slh1 (predicted)
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001022481.2	NP_596560.1	putative ATP-dependent RNA helicase Slh1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ascc3	XP_002664072.3
Magnaporthe oryzae (rice blast fungus)	MGG_06124	XP_003711991.1
Drosophila melanogaster (fruit fly)	CG5205	NP_650472.2
Saccharomyces cerevisiae (baker's yeast)	SLH1	NP_011787.4
Neurospora crassa	NCU08267	XP_961895.1
Oryza sativa (rice)	Os03g0213400	NP_001049362.1
Bos taurus (cattle)	ASCC3	NP_001193047.1
Mus musculus (house mouse)	Ascc3	NP_932124.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001234	XP_321922.5
Arabidopsis thaliana (thale cress)	AT5G61140	NP_001190584.1
Canis lupus familiaris (dog)	ASCC3	XP_854167.1
Gallus gallus (chicken)	ASCC3	XP_419816.1
Kluyveromyces lactis	KLLA0A09889g	XP_451432.1
Eremothecium gossypii	AGOS_ABR142W	NP_983089.2
Schizosaccharomyces pombe (fission yeast)	mug81	NP_596560.1
Homo sapiens (human)	ASCC3	NP_006819.2
Pan troglodytes (chimpanzee)	ASCC3	XP_518652.3
	ASCC3	XP_002803889.1
Rattus norvegicus (Norway rat)	Ascc3	NP_001263986.1
Caenorhabditis elegans (roundworm)	CELE_Y54E2A.4	NP_497060.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mug81 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mug81 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc100552	NM_001022481.2 Latest version!	Schizosaccharomyces pombe putative ATP-dependent RNA helicase Slh1 (mug81), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mug81 ( NM_001022481.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596560.1 Schizosaccharomyces pombe putative ATP-dependent RNA helicase Slh1 (mug81), mRNA.

Price & Availability↑

CloneID	OSc100552
Clone ID Related Accession (Same CDS sequence)	NM_001022481.2
Accession Version	NM_001022481.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5808bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	putative ATP-dependent RNA helicase Slh1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001022481.1.

ATGGAAGTTG AGTTAAATTA TGTAGTGAAT CACTTAGAAA AGCTAGGTCC TGCTAGTTTT 
TGTGATACTC CATATAGCTT TTCGCTGAGT GATTCGAATG CTGAGCTAGG TGCTTTATCG 
TTAAAAGTTG ACGAAATTTT GAAAACAAAT TATAATTTAA TCAATCCCGA GGACGTTACT 
GACTCCGACA ATAATGAATT TGCTCTTAAA GATTTAACTT GGCTGCAAAA TTGCTGCAAT 
GAAATTTCTC AATCTTCAAG TACTGAACTT GATGCTTCAG TACTATTTGA AGCAGTCATT 
ATGAGCCTAA AAGCGACTGA AGATCAATGT GCCATTCAAG AAGATTTACT GAATCTTGTT 
GGACTTGATC ATATTGACTT GATTTCAGAT ATCGTCGCTA ATTCGTCCAA TTTAATAGAA 
GAATACATGA ATCAAAATGA TACTTCAATT GCTGCGCAAC TTTCTGATGG CTATACCTCT 
GAAGCTGGTT CTTCTGCTAC GCATGGTCAG GGATTATTAG ACAGTTTAAA AAGTAGGCCG 
AGAAGATTTA GTCGGTCTCG TGATAACAGA GGTCCTTTAT TTACTGGACA GCAAGTTTTT 
GAAGTGGAAA AATATCCTCA TGTGTATGGG GATAAAAGGC TTGGTAATAC TATTAGCGTC 
ATTGGGAAAA AGTTTGCATT ACCGGCTGGT AGTGAAAGAG AAGATTACCA GAAATATGAA 
GAGATTATTG TTCCTCATGC TCAGCGTGCT CCACAAATGC AAGGGGAAAA GTTGTTAGAA 
ATTTCTTCGA TGGATATTCT TTGTCGAAAA ACATTCCTTA GCTACCAAAC TTTGAACAGA 
ATCCAATCGC TTGTCTATCC TATCGCCTAC AAGACAAACG AGAATATGTT AATTTGTGCA 
CCTACAGGTG CAGGAAAAAC TGATGTTGCT CTGCTCGCAA TGTTGCAAAC AATCTCCAAC 
TATGTAGAAA GTATGAATCT TATGGATGAA TCGGAACCTC TAGATGTTCA TAGAGATGAT 
TTTAAGATTG TCTACATTGC TCCTATGAAG GCATTGGCTG CTGAGGTTGT AGAGAAAATG 
GGAAAACGTC TCGCTTGGTT GGGATTGAAA ACTAGAGAAC TTACTGGTGA TATGCAACTA 
ACCAAAACGG AAATAGCAGA AACTCAGATA CTTGTTACAA CACCTGAAAA ATGGGACGTA 
GTTACTAGAA AAAGCGTCGG CGACACTCAG TTAGCCGAGA AAGTACGTTT GGTTATCATT 
GATGAAGTAC ATATGCTTCA TGATGAACGT GGTGCTGTTA TTGAATCACT TGTCGCTAGG 
ACACAAAGGC TGGTAGAAAC AAGTCAACAG ATGATTAGAA TTGTTGGTCT TTCTGCCACA 
CTTCCAAACT ACCTTGATGT AGCTGATTTT TTAGGCGTCA ATAGGTACAA GGGATTGTTT 
TATTTTTCAT CTGCATTTCG TCCATGCCCT ATTGAACAGC ATTTCATCGG TGCTAAAGGT 
TCTCCTAAAA TAGTAAACTC AAATATTGAT GAAGCTTGTT TTGATAAAGT TTTGAAACTG 
ATTCAGGAAG GCCATCAGGT AATGATATTT GTACATTCTA GGAAGGAAAC CATTAATTCA 
GCTAAGAAGT TGAGGGAACA ATTTTTCCAT GAGGGAGAGG CTGATTTGTT GGACAACTCA 
CAACATGAAA AATATAGTCT AGCTCAAAGA GATGTTTCAA AGTCAAAAAA CAAAGAGTTA 
AAAGAACTTT TCAAATACAG TATGGGGATT CACAACGCTG GCATGTTACG ATCGGACCGA 
CATCTTACTG AACGTTTGTT TTCTATGGGT ATTCTTAAAA TTTTATGCTG TACTGCTACC 
CTAGCATGGG GTGTTAATTT ACCGGCTTAT GCTGTATTAA TTAAAGGAAC TCAGCTTTAT 
GATCCACAAA AGGGTTCTTT TGTAGATCTT GGCGTGTTGG ACGTTTTACA AATATTCGGT 
CGTGCTGGTC GTCCTCAGTT TGAAAGTTCG GCAGTCGCGT ACATAATTAC GACACATGAT 
AAACTTTCTC ATTACATCTC CGTTGTAACA CAACAAAGCC CTATTGAATC TCGTTTCACC 
GATAGATTGG TTGACAACTT AAACGCTGAA GTTTCTTTAG GAACGGTAAC TAATATTGAC 
GAAGCTGTAT CGTGGTTAGG TTATACTTAT CTTTATATCA GGATGAGACG TAATCCACTT 
GTATACGGTA TTGCTTATGA CGAACTTGTT GAGGATCCTT TACTAGGCAG CAAGCGAAGA 
GAATTGGTAT CTGTAGCGGC AGGAAGGCTG GCAGATAATC AAATGATCGT TTACAATAAG 
AAAAACGGAT ACCTGATTCC TAAAGATTTA GGTAGAATCG CGTCCAATTA CTATATCAAT 
TATCAAACTG TCTCAACATT AAATAATTTA TTGAAAAGCA AAATGTCGGA AGCAGACATT 
ATAGCTCTTT TGAGCCAATG CAGCGAGTTT TCCCAAATCA AATCGAGAGA AAATGAGCAT 
CGTGAGCTTG AATCACTAAT GGAAAACAGT AGTCCTTGTC AGTTGAGGGA TAGTATTTCG 
AATACATCAG GAAAAGTTAA CGTTATTCTG CAGTCATACA TTTCTCGTGC GCATGTAGAA 
GATTTTACCC TAACATCAGA TACAAATTAT GTTGCACAAA ACGCGGGTCG AATAACTAGG 
GCTCTTTTCG AAATTGCAAT GTCAAGGACT TGGGCCTCGG CATTTACTAT CCTTAGCTTA 
AACAAGTCAA TTGATAGGAG GCAGTGGTCT TTTGAACACC CCTTACTTCA GTTTGATCTT 
CCACATGACC TTGCAGTCAA AGTGGAAAAT CAGTGTGGGT CTTTATCATT AGAAGAATTG 
TCGGACATGT CTACAGGAGA ACTTGGTGAT CTTATTCATA ACCGAAAAAT GGGCCCTACA 
GTTAAAAAAT TTATTTCCAA ATTACCTCTT TTAAACATTA ATGTTGATTT GCTACCTTTA 
ACGAAAAACG TTTTGCGACT TGTATTAAAT ATCACTCCCA ATTTTAATTG GGACATGAGA 
TATCATGGAA ACAGTCAAAT GTTTTGGATA TTTGTTGAAG ATAGTAACGG TCTTGAGATT 
CTTCATCATG AACAGTTGCT CCTAAATAAA CGGAACGTTA GCACTTCTCA TTTACTATCC 
TTTACCATAC CTGTGTCTAA TCCATTACCC TCACAACTAT ACATTATTGC AGTATCAGAT 
AAATGGCTAG GCGCTGAAAC AGTTACCCCG GTTTCTCTTT CTAATGTTGT TTTTCATGAC 
GATTCCAATC CCATTACAGA ATTATTGGAT CTTCAACCTT TACCAATTAC GGCTTTGCAC 
GATCCAGTGC TAGAAGGTAT ATGCGCTAAA AGATTCTCGT TTTTTAATGC AGTCCAAACT 
CAATTTTTTC ACACTATTTA TCACACCGAT ACAAATATTT TCGTTGGTGC ACCTACTGGA 
TCTGGTAAAA CTATGGCCGC AGAATTAGCT ACTTGGAGAG CTCTTCACAA TTATCCTAAG 
TCGAAAGTAG TTTATATTGC TCCAATGAAA GCTTTAGTCA AGGAAAGAGT AAAGGACTGG 
GGACACAGGT TAGTTGAACC AATGGGGATT AGTATGATTG AATTAACTGG TGATACTAAC 
CCGGATGTTA AGGCTGTTAC GAATGCAAAT ATCATAATTA CAACACCGGA AAAATGGGAT 
GGTATTACTA GAAGTTGGAA ATCTAGGAAG TATGTTCAGG ATGTCTCATT AATTATCCTT 
GACGAAATCC ATTTATTAGG TAGCGATCGA GGTCCTGTCC TTGAAATGAT TGTCTCCCGA 
ATGAATTATG TTGCTTCTCA AACTAATAAG AAAGTTAGAG TTCTTGGTTT ATCTACTGCT 
GTTGCTAACG CTAACGATCT TGCAAATTGG CTTAACATAA GAGATGGTCT ATTTAATTTT 
AGGCATTCCG TGCGCCCAGT TCCTTTAGAA ATATATATCG ATGGATTCCC TGGTAGAGCC 
TATTGCCCAA GGATGATGTC GATGAATAAG CCTGCTTTTC AAGCCATCAA AACGCACTCT 
CCTACCCAAC CTGTTTTAAT TTTTGTTTCG TCGCGCCGTC AAACTAGATT AACTGCAAAA 
GATTTGATAG CTTTTTGTGG TTTAGAAGAC AATCCTAGGA GATTTTTGTA CATGGATGAA 
GAAGAATTAG AAATGATTGT TTCAGAAGTT GAAGATAAAA GCCTCAAGTT GGCTTTACCA 
TTTGGTATAG CACTTCACCA TGCTGGTTTG ACTGAAAATG ATAGAAAAAT TTCCGAAGAG 
CTTTTTGTAA ATAATAAAGT TCAGATTCTC ATCGCCACCA GCACTCTTGC ATGGGGTGTA 
AATACGCCGG CACATTTGGT AATTGTGAAA GGAACAGAGT ATTACGATGC TAAAATTGGA 
GGCTATAAAG ACATGGATTT GACGGATGTG CTTCAAATGT TAGGGCGTGC AGGACGACCA 
CAATTTGACA ATTCTGGTGT GGCACGTATA TTTGTACAGG ATATTAAGAA GTCGTTTTAT 
AAGCACTTTC TACATAGTGG ATTTCCAGTA GAGTCTTATT TACATAAAGT ATTGGATAAC 
CATTTAAATG CCGAGATTGC TACGGGCACT ATAGATTGCA TACAGGGTGC AATGGATTTT 
TTAACGTGCA CTTACTTCTA TCGAAGAGTT CATCAAAATC CCGTTTATTA CGGTGCTGAT 
GGAGATGACC AAAAGAGTAT AGATACTTAT TTATCGAAAC TTGTTGTGAC TGCATTTAAT 
GAACTTGAAA AGTCAGCTTG CATATATCGT GTCAACGAAG AAACCTATGC ACCTACAACT 
TTGGGTCGTA TAGTTAGTTA CTATTATCTT TTTCATACCA CCATCAGAAA CTTCGTTCAA 
AAAATAACTG AGAACGCTGA GTTCGATCTT GCGTTGCAAT TGCTTGCTGA AGCATCTGAG 
TTTGACGATC TCGCTATTCG TCACAACGAA GATTTGATAA ACATAGAGAT TAATAAATCT 
CTGAAATATT CGGCTGCTTG CCTAAACCTT CCTATGGTCG ATGCTCATGT TAAGGCATTT 
ATATTAACTC AAGCTCACAT GGCAAGATTG AAACTTCCAG TGGATGATTA TGTTACTGAT 
ACTAGCACGG TTTTAGATCA GGTTATTCGT ATTATACAGT CTTATATTGA TGTGTCCGCT 
GAGCTAGGCT ACTCCCATGT CTGTTTACAG TACATATCTC TAATGCAGTG TCTTAAACAG 
GCTTGCTATC CGAGTGAGAT TTACAGAGCT TCATTACCTG GACTTAATGC GTCTAGTGAA 
AAGGAAGCAA GAGATTATCT CAACAAATTT GCAGGAAATA AAACTGATGA GTTATATCAA 
ATGCTATGTA ATGACCCTAA CGTTTTTGAT ATAGAAAGTC TGGTCAATAG TTTAATTTCG 
TATCCTAAAA TGAACATTGA AGTTTCTCAA TCGTCTTCAG ATAAGCTACT CCTTTATCTA 
CGACGCCTCA ATCAGCCGCT CAACCCCGAT TTTTACATTT TTGCGCCGTT GTTCCCTAAA 
CCTCAAAGTG AGGGATTTTT CGTTCTCATC ATAGATTCAG AAACTCAAGA ACTGTTCGCG 
ATACGTCGGG CCTCATTTGC TGGGCGAAGA AACGATGATT CAATTCGTTT ATCCCTTCGG 
ATTTCAATGG ATATTCCACC AACTTGCCGC AACAGAAATG TAAAAGTCAT GGTTGTTTGT 
GATGGATACC CATTAATTTA CGAACATAAA ATTGTATTGA TGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596560.1
CDS	123..5930
Translation	MEVELNYVVNHLEKLGPASFCDTPYSFSLSDSNAELGALSLKVDEILKTNYNLINPEDVTDSDNNEFALKDLTWLQNCCNEISQSSSTELDASVLFEAVIMSLKATEDQCAIQEDLLNLVGLDHIDLISDIVANSSNLIEEYMNQNDTSIAAQLSDGYTSEAGSSATHGQGLLDSLKSRPRRFSRSRDNRGPLFTGQQVFEVEKYPHVYGDKRLGNTISVIGKKFALPAGSEREDYQKYEEIIVPHAQRAPQMQGEKLLEISSMDILCRKTFLSYQTLNRIQSLVYPIAYKTNENMLICAPTGAGKTDVALLAMLQTISNYVESMNLMDESEPLDVHRDDFKIVYIAPMKALAAEVVEKMGKRLAWLGLKTRELTGDMQLTKTEIAETQILVTTPEKWDVVTRKSVGDTQLAEKVRLVIIDEVHMLHDERGAVIESLVARTQRLVETSQQMIRIVGLSATLPNYLDVADFLGVNRYKGLFYFSSAFRPCPIEQHFIGAKGSPKIVNSNIDEACFDKVLKLIQEGHQVMIFVHSRKETINSAKKLREQFFHEGEADLLDNSQHEKYSLAQRDVSKSKNKELKELFKYSMGIHNAGMLRSDRHLTERLFSMGILKILCCTATLAWGVNLPAYAVLIKGTQLYDPQKGSFVDLGVLDVLQIFGRAGRPQFESSAVAYIITTHDKLSHYISVVTQQSPIESRFTDRLVDNLNAEVSLGTVTNIDEAVSWLGYTYLYIRMRRNPLVYGIAYDELVEDPLLGSKRRELVSVAAGRLADNQMIVYNKKNGYLIPKDLGRIASNYYINYQTVSTLNNLLKSKMSEADIIALLSQCSEFSQIKSRENEHRELESLMENSSPCQLRDSISNTSGKVNVILQSYISRAHVEDFTLTSDTNYVAQNAGRITRALFEIAMSRTWASAFTILSLNKSIDRRQWSFEHPLLQFDLPHDLAVKVENQCGSLSLEELSDMSTGELGDLIHNRKMGPTVKKFISKLPLLNINVDLLPLTKNVLRLVLNITPNFNWDMRYHGNSQMFWIFVEDSNGLEILHHEQLLLNKRNVSTSHLLSFTIPVSNPLPSQLYIIAVSDKWLGAETVTPVSLSNVVFHDDSNPITELLDLQPLPITALHDPVLEGICAKRFSFFNAVQTQFFHTIYHTDTNIFVGAPTGSGKTMAAELATWRALHNYPKSKVVYIAPMKALVKERVKDWGHRLVEPMGISMIELTGDTNPDVKAVTNANIIITTPEKWDGITRSWKSRKYVQDVSLIILDEIHLLGSDRGPVLEMIVSRMNYVASQTNKKVRVLGLSTAVANANDLANWLNIRDGLFNFRHSVRPVPLEIYIDGFPGRAYCPRMMSMNKPAFQAIKTHSPTQPVLIFVSSRRQTRLTAKDLIAFCGLEDNPRRFLYMDEEELEMIVSEVEDKSLKLALPFGIALHHAGLTENDRKISEELFVNNKVQILIATSTLAWGVNTPAHLVIVKGTEYYDAKIGGYKDMDLTDVLQMLGRAGRPQFDNSGVARIFVQDIKKSFYKHFLHSGFPVESYLHKVLDNHLNAEIATGTIDCIQGAMDFLTCTYFYRRVHQNPVYYGADGDDQKSIDTYLSKLVVTAFNELEKSACIYRVNEETYAPTTLGRIVSYYYLFHTTIRNFVQKITENAEFDLALQLLAEASEFDDLAIRHNEDLINIEINKSLKYSAACLNLPMVDAHVKAFILTQAHMARLKLPVDDYVTDTSTVLDQVIRIIQSYIDVSAELGYSHVCLQYISLMQCLKQACYPSEIYRASLPGLNASSEKEARDYLNKFAGNKTDELYQMLCNDPNVFDIESLVNSLISYPKMNIEVSQSSSDKLLLYLRRLNQPLNPDFYIFAPLFPKPQSEGFFVLIIDSETQELFAIRRASFAGRRNDDSIRLSLRISMDIPPTCRNRNVKVMVVCDGYPLIYEHKIVLMI

Target ORF information:

RefSeq Version	NM_001022481.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe putative ATP-dependent RNA helicase Slh1 (mug81), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001022481.2

ORF Insert Sequence:

ATGGAAGTTG AGTTAAATTA TGTAGTGAAT CACTTAGAAA AGCTAGGTCC TGCTAGTTTT 
TGTGATACTC CATATAGCTT TTCGCTGAGT GATTCGAATG CTGAGCTAGG TGCTTTATCG 
TTAAAAGTTG ACGAAATTTT GAAAACAAAT TATAATTTAA TCAATCCCGA GGACGTTACT 
GACTCCGACA ATAATGAATT TGCTCTTAAA GATTTAACTT GGCTGCAAAA TTGCTGCAAT 
GAAATTTCTC AATCTTCAAG TACTGAACTT GATGCTTCAG TACTATTTGA AGCAGTCATT 
ATGAGCCTAA AAGCGACTGA AGATCAATGT GCCATTCAAG AAGATTTACT GAATCTTGTT 
GGACTTGATC ATATTGACTT GATTTCAGAT ATCGTCGCTA ATTCGTCCAA TTTAATAGAA 
GAATACATGA ATCAAAATGA TACTTCAATT GCTGCGCAAC TTTCTGATGG CTATACCTCT 
GAAGCTGGTT CTTCTGCTAC GCATGGTCAG GGATTATTAG ACAGTTTAAA AAGTAGGCCG 
AGAAGATTTA GTCGGTCTCG TGATAACAGA GGTCCTTTAT TTACTGGACA GCAAGTTTTT 
GAAGTGGAAA AATATCCTCA TGTGTATGGG GATAAAAGGC TTGGTAATAC TATTAGCGTC 
ATTGGGAAAA AGTTTGCATT ACCGGCTGGT AGTGAAAGAG AAGATTACCA GAAATATGAA 
GAGATTATTG TTCCTCATGC TCAGCGTGCT CCACAAATGC AAGGGGAAAA GTTGTTAGAA 
ATTTCTTCGA TGGATATTCT TTGTCGAAAA ACATTCCTTA GCTACCAAAC TTTGAACAGA 
ATCCAATCGC TTGTCTATCC TATCGCCTAC AAGACAAACG AGAATATGTT AATTTGTGCA 
CCTACAGGTG CAGGAAAAAC TGATGTTGCT CTGCTCGCAA TGTTGCAAAC AATCTCCAAC 
TATGTAGAAA GTATGAATCT TATGGATGAA TCGGAACCTC TAGATGTTCA TAGAGATGAT 
TTTAAGATTG TCTACATTGC TCCTATGAAG GCATTGGCTG CTGAGGTTGT AGAGAAAATG 
GGAAAACGTC TCGCTTGGTT GGGATTGAAA ACTAGAGAAC TTACTGGTGA TATGCAACTA 
ACCAAAACGG AAATAGCAGA AACTCAGATA CTTGTTACAA CACCTGAAAA ATGGGACGTA 
GTTACTAGAA AAAGCGTCGG CGACACTCAG TTAGCCGAGA AAGTACGTTT GGTTATCATT 
GATGAAGTAC ATATGCTTCA TGATGAACGT GGTGCTGTTA TTGAATCACT TGTCGCTAGG 
ACACAAAGGC TGGTAGAAAC AAGTCAACAG ATGATTAGAA TTGTTGGTCT TTCTGCCACA 
CTTCCAAACT ACCTTGATGT AGCTGATTTT TTAGGCGTCA ATAGGTACAA GGGATTGTTT 
TATTTTTCAT CTGCATTTCG TCCATGCCCT ATTGAACAGC ATTTCATCGG TGCTAAAGGT 
TCTCCTAAAA TAGTAAACTC AAATATTGAT GAAGCTTGTT TTGATAAAGT TTTGAAACTG 
ATTCAGGAAG GCCATCAGGT AATGATATTT GTACATTCTA GGAAGGAAAC CATTAATTCA 
GCTAAGAAGT TGAGGGAACA ATTTTTCCAT GAGGGAGAGG CTGATTTGTT GGACAACTCA 
CAACATGAAA AATATAGTCT AGCTCAAAGA GATGTTTCAA AGTCAAAAAA CAAAGAGTTA 
AAAGAACTTT TCAAATACAG TATGGGGATT CACAACGCTG GCATGTTACG ATCGGACCGA 
CATCTTACTG AACGTTTGTT TTCTATGGGT ATTCTTAAAA TTTTATGCTG TACTGCTACC 
CTAGCATGGG GTGTTAATTT ACCGGCTTAT GCTGTATTAA TTAAAGGAAC TCAGCTTTAT 
GATCCACAAA AGGGTTCTTT TGTAGATCTT GGCGTGTTGG ACGTTTTACA AATATTCGGT 
CGTGCTGGTC GTCCTCAGTT TGAAAGTTCG GCAGTCGCGT ACATAATTAC GACACATGAT 
AAACTTTCTC ATTACATCTC CGTTGTAACA CAACAAAGCC CTATTGAATC TCGTTTCACC 
GATAGATTGG TTGACAACTT AAACGCTGAA GTTTCTTTAG GAACGGTAAC TAATATTGAC 
GAAGCTGTAT CGTGGTTAGG TTATACTTAT CTTTATATCA GGATGAGACG TAATCCACTT 
GTATACGGTA TTGCTTATGA CGAACTTGTT GAGGATCCTT TACTAGGCAG CAAGCGAAGA 
GAATTGGTAT CTGTAGCGGC AGGAAGGCTG GCAGATAATC AAATGATCGT TTACAATAAG 
AAAAACGGAT ACCTGATTCC TAAAGATTTA GGTAGAATCG CGTCCAATTA CTATATCAAT 
TATCAAACTG TCTCAACATT AAATAATTTA TTGAAAAGCA AAATGTCGGA AGCAGACATT 
ATAGCTCTTT TGAGCCAATG CAGCGAGTTT TCCCAAATCA AATCGAGAGA AAATGAGCAT 
CGTGAGCTTG AATCACTAAT GGAAAACAGT AGTCCTTGTC AGTTGAGGGA TAGTATTTCG 
AATACATCAG GAAAAGTTAA CGTTATTCTG CAGTCATACA TTTCTCGTGC GCATGTAGAA 
GATTTTACCC TAACATCAGA TACAAATTAT GTTGCACAAA ACGCGGGTCG AATAACTAGG 
GCTCTTTTCG AAATTGCAAT GTCAAGGACT TGGGCCTCGG CATTTACTAT CCTTAGCTTA 
AACAAGTCAA TTGATAGGAG GCAGTGGTCT TTTGAACACC CCTTACTTCA GTTTGATCTT 
CCACATGACC TTGCAGTCAA AGTGGAAAAT CAGTGTGGGT CTTTATCATT AGAAGAATTG 
TCGGACATGT CTACAGGAGA ACTTGGTGAT CTTATTCATA ACCGAAAAAT GGGCCCTACA 
GTTAAAAAAT TTATTTCCAA ATTACCTCTT TTAAACATTA ATGTTGATTT GCTACCTTTA 
ACGAAAAACG TTTTGCGACT TGTATTAAAT ATCACTCCCA ATTTTAATTG GGACATGAGA 
TATCATGGAA ACAGTCAAAT GTTTTGGATA TTTGTTGAAG ATAGTAACGG TCTTGAGATT 
CTTCATCATG AACAGTTGCT CCTAAATAAA CGGAACGTTA GCACTTCTCA TTTACTATCC 
TTTACCATAC CTGTGTCTAA TCCATTACCC TCACAACTAT ACATTATTGC AGTATCAGAT 
AAATGGCTAG GCGCTGAAAC AGTTACCCCG GTTTCTCTTT CTAATGTTGT TTTTCATGAC 
GATTCCAATC CCATTACAGA ATTATTGGAT CTTCAACCTT TACCAATTAC GGCTTTGCAC 
GATCCAGTGC TAGAAGGTAT ATGCGCTAAA AGATTCTCGT TTTTTAATGC AGTCCAAACT 
CAATTTTTTC ACACTATTTA TCACACCGAT ACAAATATTT TCGTTGGTGC ACCTACTGGA 
TCTGGTAAAA CTATGGCCGC AGAATTAGCT ACTTGGAGAG CTCTTCACAA TTATCCTAAG 
TCGAAAGTAG TTTATATTGC TCCAATGAAA GCTTTAGTCA AGGAAAGAGT AAAGGACTGG 
GGACACAGGT TAGTTGAACC AATGGGGATT AGTATGATTG AATTAACTGG TGATACTAAC 
CCGGATGTTA AGGCTGTTAC GAATGCAAAT ATCATAATTA CAACACCGGA AAAATGGGAT 
GGTATTACTA GAAGTTGGAA ATCTAGGAAG TATGTTCAGG ATGTCTCATT AATTATCCTT 
GACGAAATCC ATTTATTAGG TAGCGATCGA GGTCCTGTCC TTGAAATGAT TGTCTCCCGA 
ATGAATTATG TTGCTTCTCA AACTAATAAG AAAGTTAGAG TTCTTGGTTT ATCTACTGCT 
GTTGCTAACG CTAACGATCT TGCAAATTGG CTTAACATAA GAGATGGTCT ATTTAATTTT 
AGGCATTCCG TGCGCCCAGT TCCTTTAGAA ATATATATCG ATGGATTCCC TGGTAGAGCC 
TATTGCCCAA GGATGATGTC GATGAATAAG CCTGCTTTTC AAGCCATCAA AACGCACTCT 
CCTACCCAAC CTGTTTTAAT TTTTGTTTCG TCGCGCCGTC AAACTAGATT AACTGCAAAA 
GATTTGATAG CTTTTTGTGG TTTAGAAGAC AATCCTAGGA GATTTTTGTA CATGGATGAA 
GAAGAATTAG AAATGATTGT TTCAGAAGTT GAAGATAAAA GCCTCAAGTT GGCTTTACCA 
TTTGGTATAG CACTTCACCA TGCTGGTTTG ACTGAAAATG ATAGAAAAAT TTCCGAAGAG 
CTTTTTGTAA ATAATAAAGT TCAGATTCTC ATCGCCACCA GCACTCTTGC ATGGGGTGTA 
AATACGCCGG CACATTTGGT AATTGTGAAA GGAACAGAGT ATTACGATGC TAAAATTGGA 
GGCTATAAAG ACATGGATTT GACGGATGTG CTTCAAATGT TAGGGCGTGC AGGACGACCA 
CAATTTGACA ATTCTGGTGT GGCACGTATA TTTGTACAGG ATATTAAGAA GTCGTTTTAT 
AAGCACTTTC TACATAGTGG ATTTCCAGTA GAGTCTTATT TACATAAAGT ATTGGATAAC 
CATTTAAATG CCGAGATTGC TACGGGCACT ATAGATTGCA TACAGGGTGC AATGGATTTT 
TTAACGTGCA CTTACTTCTA TCGAAGAGTT CATCAAAATC CCGTTTATTA CGGTGCTGAT 
GGAGATGACC AAAAGAGTAT AGATACTTAT TTATCGAAAC TTGTTGTGAC TGCATTTAAT 
GAACTTGAAA AGTCAGCTTG CATATATCGT GTCAACGAAG AAACCTATGC ACCTACAACT 
TTGGGTCGTA TAGTTAGTTA CTATTATCTT TTTCATACCA CCATCAGAAA CTTCGTTCAA 
AAAATAACTG AGAACGCTGA GTTCGATCTT GCGTTGCAAT TGCTTGCTGA AGCATCTGAG 
TTTGACGATC TCGCTATTCG TCACAACGAA GATTTGATAA ACATAGAGAT TAATAAATCT 
CTGAAATATT CGGCTGCTTG CCTAAACCTT CCTATGGTCG ATGCTCATGT TAAGGCATTT 
ATATTAACTC AAGCTCACAT GGCAAGATTG AAACTTCCAG TGGATGATTA TGTTACTGAT 
ACTAGCACGG TTTTAGATCA GGTTATTCGT ATTATACAGT CTTATATTGA TGTGTCCGCT 
GAGCTAGGCT ACTCCCATGT CTGTTTACAG TACATATCTC TAATGCAGTG TCTTAAACAG 
GCTTGCTATC CGAGTGAGAT TTACAGAGCT TCATTACCTG GACTTAATGC GTCTAGTGAA 
AAGGAAGCAA GAGATTATCT CAACAAATTT GCAGGAAATA AAACTGATGA GTTATATCAA 
ATGCTATGTA ATGACCCTAA CGTTTTTGAT ATAGAAAGTC TGGTCAATAG TTTAATTTCG 
TATCCTAAAA TGAACATTGA AGTTTCTCAA TCGTCTTCAG ATAAGCTACT CCTTTATCTA 
CGACGCCTCA ATCAGCCGCT CAACCCCGAT TTTTACATTT TTGCGCCGTT GTTCCCTAAA 
CCTCAAAGTG AGGGATTTTT CGTTCTCATC ATAGATTCAG AAACTCAAGA ACTGTTCGCG 
ATACGTCGGG CCTCATTTGC TGGGCGAAGA AACGATGATT CAATTCGTTT ATCCCTTCGG 
ATTTCAATGG ATATTCCACC AACTTGCCGC AACAGAAATG TAAAAGTCAT GGTTGTTTGT 
GATGGATACC CATTAATTTA CGAACATAAA ATTGTATTGA TGATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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mug81 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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