ORF » Species Summary » Schizosaccharomyces pombe » SPCC548.05c cDNA ORF clone

SPCC548.05c cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

  • Gene

  • Clones

  • gRNAs

The following SPCC548.05c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPCC548.05c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc99892 NM_001022739.2
Latest version!		 Schizosaccharomyces pombe putative ubiquitin-protein ligase E3 (SPCC548.05c), mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    SPCC548.05c ( NM_001022739.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_587745.1 Schizosaccharomyces pombe putative ubiquitin-protein ligase E3 (SPCC548.05c), mRNA.
    Price & Availability↑
    CloneID OSc99892
    Clone ID Related Accession (Same CDS sequence) NM_001022739.2
    Accession Version NM_001022739.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative ubiquitin-protein ligase E3
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001022739.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGTTAAAC GCAGTTCTCA TCGCCAAGTA GTTTTGGATG AAGACGATGA AGAAAATTAT 
    AATAATAACT TGGACGATGA GAAAATGGAG GTTCTATTAA TTCCACAATC CAACAGCACA 
    ACTTTTGCGT CTAGTGATGC AACTCAAATG TACAAAAAGT CTCGCATTAG CAGTAATTCA 
    GAAAATAAAA AACAAATCCC CGATACAAAA ACTTTGCTCG AAACGTTCCA GAAAATAAAA 
    AAAACTCTGG AATGTCCCAT TTGCACAGAA GCTTTACAAA GACCTTTTAC CACTCATTGC 
    GGTCATACAT ATTGTTACGA GTGTCTTCTA AATTGGTTAA AAGAGTCAAA ATCTTGCCCA 
    ACTTGTCGCC AAAAACTGTA TACACAACCT TCACCAGCAT ATCTTGTATA TGAAATAATG 
    AATGTGGTTG CTGCCTCAAA CAGTGGCTTC CCACTTGTGG GAATCAATGA AAATCCAGCA 
    AAGAAGCAAA AGGAGGTATT GTTTGATGGG ATGTTTAAAC AAGAAGATTC TCATTACCCA 
    AGAAGTATCC TTGTTGATTC TGAAGATGGA GTTTTACGAT GCGCTCGTTG CCAATGGGAA 
    TTGGAAAATC CGTACCATTG CGATCATTGC GGTTTTCAAA TATCGGATGA CCAAGATTCT 
    GGTCGTGAAT GGTTTTGGGA TGGAGAAAAT GCTGAGAGTG ATTCCAGCCT AAATGGCGAT 
    AACACCCGTG GTGGGAATAT CTCGACTAAC CGCGCATTTA ATAATATGGG ACATGCGCCA 
    ATAACTGCAG TACCACAAGA TTGGCTGAGA TTTGACGAAG GAGAAGAATT TGTGGGGTCT 
    GATTTAGAAT CTGATTTTTC TGGTCCAGGT GAGTACGATG TTGATGATGG ATTTATAGAT 
    AATCGTGCTA CAAGCCAATT ATCTCCTGTC GAAAGTGACG ATGACTTCGT AGCTCCTGTC 
    AATGGAAGTA ATGGAAATGG TATTACTGCT TTGGATAGCA CAGATAGTGA AGAGATTGAT 
    ATTATGAACG GCTTCGATGA AGAGCGCGAC TCTGGAGGGA CGAATATGGT ATCTCGATCT 
    GAGACCTGTT ATAACGATGG GCAAAGATAC GATGAGTTAC GAAGAGAGCT TGCTGACATT 
    CAGAATGAAA GTTTGGATTC TCTCAATTCT TCCTCAAATA ATTCTCCTTC TCATAACAAT 
    ATTCATTCCA GGCAACATCC ATTCTCATCA GATGAGGATG AAGGAAATAT TGTAACTAAT 
    GGAACAGGCC TCCGTAGTTC ACAATCATCC AGCCAGAATA GAGGATTTGA TCTTGAACCC 
    TATTCCGAGG TATTCACTGC AAGCTATCCA CGTCGGCAAG CTCGGCGAAC TCGTACAATA 
    CAACTAGACT CTGACGAAGA ATCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_587745.1
    CDS114..1520
    Translation

    Target ORF information:

    RefSeq Version NM_001022739.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative ubiquitin-protein ligase E3 (SPCC548.05c), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001022739.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGTTAAAC GCAGTTCTCA TCGCCAAGTA GTTTTGGATG AAGACGATGA AGAAAATTAT 
    AATAATAACT TGGACGATGA GAAAATGGAG GTTCTATTAA TTCCACAATC CAACAGCACA 
    ACTTTTGCGT CTAGTGATGC AACTCAAATG TACAAAAAGT CTCGCATTAG CAGTAATTCA 
    GAAAATAAAA AACAAATCCC CGATACAAAA ACTTTGCTCG AAACGTTCCA GAAAATAAAA 
    AAAACTCTGG AATGTCCCAT TTGCACAGAA GCTTTACAAA GACCTTTTAC CACTCATTGC 
    GGTCATACAT ATTGTTACGA GTGTCTTCTA AATTGGTTAA AAGAGTCAAA ATCTTGCCCA 
    ACTTGTCGCC AAAAACTGTA TACACAACCT TCACCAGCAT ATCTTGTATA TGAAATAATG 
    AATGTGGTTG CTGCCTCAAA CAGTGGCTTC CCACTTGTGG GAATCAATGA AAATCCAGCA 
    AAGAAGCAAA AGGAGGTATT GTTTGATGGG ATGTTTAAAC AAGAAGATTC TCATTACCCA 
    AGAAGTATCC TTGTTGATTC TGAAGATGGA GTTTTACGAT GCGCTCGTTG CCAATGGGAA 
    TTGGAAAATC CGTACCATTG CGATCATTGC GGTTTTCAAA TATCGGATGA CCAAGATTCT 
    GGTCGTGAAT GGTTTTGGGA TGGAGAAAAT GCTGAGAGTG ATTCCAGCCT AAATGGCGAT 
    AACACCCGTG GTGGGAATAT CTCGACTAAC CGCGCATTTA ATAATATGGG ACATGCGCCA 
    ATAACTGCAG TACCACAAGA TTGGCTGAGA TTTGACGAAG GAGAAGAATT TGTGGGGTCT 
    GATTTAGAAT CTGATTTTTC TGGTCCAGGT GAGTACGATG TTGATGATGG ATTTATAGAT 
    AATCGTGCTA CAAGCCAATT ATCTCCTGTC GAAAGTGACG ATGACTTCGT AGCTCCTGTC 
    AATGGAAGTA ATGGAAATGG TATTACTGCT TTGGATAGCA CAGATAGTGA AGAGATTGAT 
    ATTATGAACG GCTTCGATGA AGAGCGCGAC TCTGGAGGGA CGAATATGGT ATCTCGATCT 
    GAGACCTGTT ATAACGATGG GCAAAGATAC GATGAGTTAC GAAGAGAGCT TGCTGACATT 
    CAGAATGAAA GTTTGGATTC TCTCAATTCT TCCTCAAATA ATTCTCCTTC TCATAACAAT 
    ATTCATTCCA GGCAACATCC ATTCTCATCA GATGAGGATG AAGGAAATAT TGTAACTAAT 
    GGAACAGGCC TCCGTAGTTC ACAATCATCC AGCCAGAATA GAGGATTTGA TCTTGAACCC 
    TATTCCGAGG TATTCACTGC AAGCTATCCA CGTCGGCAAG CTCGGCGAAC TCGTACAATA 
    CAACTAGACT CTGACGAAGA ATCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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