Gene Symbol | mad3 |
Entrez Gene ID | 2539219 |
Full Name | mitotic spindle checkpoint protein Mad3 |
Gene Type | protein-coding |
Organism | Schizosaccharomyces pombe(fission yeast) |
ORF » Species Summary » Schizosaccharomyces pombe » mad3 cDNA ORF clone
Gene Symbol | mad3 |
Entrez Gene ID | 2539219 |
Full Name | mitotic spindle checkpoint protein Mad3 |
Gene Type | protein-coding |
Organism | Schizosaccharomyces pombe(fission yeast) |
mRNA | Protein | Name |
---|---|---|
NM_001023035.2 | NP_588043.2 | mitotic spindle checkpoint protein Mad3 |
The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
A kinase-dead allele of the fission yeast MPS1 homolog (Mph1) is checkpoint defective and levels of anaphase promoting complex-associated Mad2 and Mad3 are dramatically reduced in this mutant.
Title: Kinase activity of fission yeast Mph1 is required for Mad2 and Mad3 to stably bind the anaphase promoting complex.
the Mad3 KEN box is a critical mediator of Cdc20-APC/C inhibition, without which neither Mad3 nor Mad2 can associate with the APC/C or inhibit anaphase onset
Title: The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C).
Bub1p-A78V is defective in both nuclear accumulation and kinetochore targeting; a threshold level of nuclear Bub1p is necessary for the nuclear accumulation of Bub3p and Mad3p
Title: The A78V mutation in the Mad3-like domain of Schizosaccharomyces pombe Bub1p perturbs nuclear accumulation and kinetochore targeting of Bub1p, Bub3p, and Mad3p and spindle assembly checkpoint function.
The following mad3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mad3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OSc99056 | |
Clone ID Related Accession (Same CDS sequence) | NM_001023035.2 | |
Accession Version | NM_001023035.2 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 933bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2018-04-02 | |
Organism | Schizosaccharomyces pombe(fission yeast) | |
Product | mitotic spindle checkpoint protein Mad3 | |
Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023035.1. |
1 | ATGGAACCAT TAGATGCTGG CAAGAACTGG GTGCATATGG ATGTCATTGA GCAGTCCAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_588043.2 |
CDS | 334..1266 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001023035.2 |
1 | ATGGAACCAT TAGATGCTGG CAAGAACTGG GTGCATATGG ATGTCATTGA GCAGTCCAAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The genome sequence of Schizosaccharomyces pombe. |