dcr1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	dcr1
Entrez Gene ID	2538930
Full Name	dicer
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001023205.2	NP_588215.2	dicer

Pathways from BioSystems

Homologies

Homo sapiens (human)	DICER1	NP_085124.2
Rattus norvegicus (Norway rat)	Dicer1	XP_006240573.1
Danio rerio (zebrafish)	dicer1	NP_001154925.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002836	XP_003436256.1
Magnaporthe oryzae (rice blast fungus)	MGG_01541	XP_003714515.1
Neurospora crassa	NCU08270	XP_961898.1
	DICER1	XP_001100868.1
Gallus gallus (chicken)	DICER1	NP_001035555.1
Drosophila melanogaster (fruit fly)	Dcr-1	NP_524453.1
Caenorhabditis elegans (roundworm)	dcr-1	NP_498761.2
Xenopus tropicalis (tropical clawed frog)	dicer1	NP_001123390.2
Canis lupus familiaris (dog)	DICER1	XP_868526.3
Bos taurus (cattle)	DICER1	NP_976235.1
Schizosaccharomyces pombe (fission yeast)	dcr1	NP_588215.2
Pan troglodytes (chimpanzee)	DICER1	XP_001154369.1
Mus musculus (house mouse)	Dicer1	NP_683750.2

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings suggest that in S. pombe Dcr1 has a genome-wide role in terminating transcription by releasing Pol II at sites of collision between transcription and replication and thus maintains genome stability.
Title: Dicer promotes transcription termination at sites of replication stress to maintain genome stability.

Studies indicate that S. pombe genome encodes one of each of the core RNAi proteins, Dicer, Argonaute and RNA-dependent RNA polymerase (dcr1, ago1, rdp1).
Title: RNAi mediates post-transcriptional repression of gene expression in fission yeast Schizosaccharomyces pombe.

Data indicate that Argonaute, loaded with longer RNA precursors, recruits Triman (SPBC29A10.09c, 3'-5' Exonuclease) to generate dicer-independent priRNAs mature priRNAs and siRNAs.
Title: Argonaute and Triman generate dicer-independent priRNAs and mature siRNAs to initiate heterochromatin formation.

these results reveal novel insights into the mechanism of nuclear retention of Dcr1 and raise the possibility that this new class of dsRBDs might generally function in nucleocytoplasmic trafficking and not substrate binding
Title: An extended dsRBD with a novel zinc-binding motif mediates nuclear retention of fission yeast Dicer.

Dcr1 has a role in post-transcriptional regulation of gene expression in Schizosaccharomyces pombe [review]
Title: Involvement of Dcr1 in post-transcriptional regulation of gene expression in Schizosaccharomyces pombe.

The following dcr1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dcr1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc99079	NM_001023205.2 Latest version!	Schizosaccharomyces pombe dicer (dcr1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dcr1 ( NM_001023205.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_588215.2 Schizosaccharomyces pombe dicer (dcr1), mRNA.

Price & Availability↑

CloneID	OSc99079
Clone ID Related Accession (Same CDS sequence)	NM_001023205.2
Accession Version	NM_001023205.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	dicer
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023205.1.

ATGGATATTT CAAGTTTTCT ACTTCCTCAA CTTTTACGTA AATATCAACA AGATGTGTAT 
AATATCGCGA GCAAGCAAAA TACTTTACTT GTTATGAGAA CGGGCGCTGG TAAGACATTA 
CTTGCTGTGA AGTTGATAAA ACAAAAGCTC GAGGAGCAAA TTTTAATCCA AGAATCAAAT 
CTTGAACATA AAAAAATATC AGTTTTTCTC GTCAACAAAG TCCCTTTGGT ATTTCAACAA 
GCGGAATACA TTCGATCTCA ACTACCGGCT AAGGTTGGCA TGTTTTATGG CGAATTATCT 
ATAGAAATGA GCGAGCAGTT GTTGACTAAT ATTATATTGA AGTATAATGT GATTGTTATT 
ACTGCAGATT TGTTCTATTT GTTTCTTGCA AGAGGTTTTC TTTCAATAAA TGATTTGAAT 
TTAATTATAT TCGACGAATG TCATCATGCA ATTGGAAATG ATGCGTATGC TCGCATCATG 
AATGATTTTT ATCACAGAGC CAAAGCAGTA TTGTCAAAAA AACATTTCAC CCTACCAAGA 
ATTTTTGGTA TGACTGCTTC ACCATTCACT GGAAAAAAAG GAAACTTATA CCATCGACTG 
TATCAATGGG AGCAATTATT TGATTCTAAA GCACACGTGG TTTCGGAAAA CGAGCTAGCC 
GATTACTTCT GTCTTCCCGA AGAAAGCTAT GTAATGTATT CCAATAAGTT GGTTGTGCCA 
CCCTCGGATT CTATTATCAA GAAATGCGAG GAAACTCTTC AAGGATGCAA GTTAATTTCT 
CGGGCTGTTA AGACTGCTTT AGCAGAAACC ATAGATATGG GTCTTTGGTT TGGGGAGCAA 
GTTTGGTTAT ATTTGGTTGA TTTTGTGGAA ACGAAAAGAT TAAAAAAAAA GGCTTTAGGG 
AAGCAGTTGT CAGATGACGA GGAACTGGCA ATTGACCGGT TAAAAATATT TGTTGAAGAT 
TGGAAAAATA ACAAATATTC AGACAATGGC CCTAGAATCC CTGTTTTTGA TTCCACTGAT 
GTTACTGATA AAGTCTTTAA ACTCTTAGAA TTGTTAAAGG CTACTTACCG CAAAAGTGAT 
AGCGTTCGTA CGGTTATTTT CGTTGAAAGA AAAGCTACGG CGTTTACTTT AAGTTTGTTT 
ATGAAAACTC TTAATCTGCC TAACATCCGC GCTCATTCTT TTATAGGACA TGGACCGTCC 
GATCAGGGTG AATTTTCTAT GACATTCAGG AGGCAAAAAG ATACCCTTCA TAAGTTTAAG 
ACTGGAAAAT ATAATGTTTT AATTGCTACT GCAGTTGCAG AAGAAGGTAT CGATGTACCA 
TCATGTAACT TAGTTATACG CTTCAATATT TGTCGGACTG TCACCCAGTA TGTCCAATCT 
CGAGGTAGAG CGAGAGCAAT GGCTTCAAAG TTTCTAATTT TTTTAAACAC AGAAGAGTTG 
TTAATTCATG AACGCATTCT ACACGAAGAA AAAAATCTTA AATTTGCCCT TTCAGAGCTC 
AGCAATTCGA ATATTTTTGA TTCATTGGTA TGTGAGGAAA GAGAACGTGT GACTGATGAT 
ATCGTCTATG AAGTTGGCGA GACTGGTGCT TTACTCACAG GGTTGTATGC AGTTAGTCTG 
CTTTATAACT TTTGTAACAC ACTTTCAAGA GACGTATACA CAAGATATTA TCCCACTTTT 
ACAGCTCAAC CCTGTCTTTC AGGTTGGTAT TGTTTTGAGG TAGAATTGCC AAAAGCCTGC 
AAAGTTCCAG CGGCTCAAGG ATCTCCCGCT AAATCAATTA GGAAAGCCAA ACAGAATGCT 
GCGTTCATCA TGTGTTTGGA TCTGATTCGT ATGGGTCTTA TAGACAAACA TTTAAAACCC 
CTAGATTTTA GAAGAAAAAT TGCCGACCTT GAAACTCTTG AGGAAGACGA GCTAAAAGAT 
GAAGGTTATA TCGAGACATA TGAGCGCTAT GTACCAAAAA GTTGGATGAA AGTTCCTGAA 
GATATTACAC GTTGCTTCGT CTCTTTACTT TATACTGATG CTAATGAAGG AGACAATCAT 
ATATTCCATC CCTTAGTGTT TGTACAAGCT CATTCATTCC CCAAAATTGA TAGCTTTATT 
CTTAATTCGA CTGTTGGCCC CCGAGTTAAA ATTGTTTTAG AAACGATTGA GGATAGTTTT 
AAGATCGATT CTCATCTGCT TGAGTTGTTA AAAAAATCAA CTCGTTATCT ACTTCAATTC 
GGTTTATCTA CTTCTCTTGA GCAACAAATA CCTACTCCTT ACTGGCTTGC GCCTTTAAAT 
TTGTCATGCA CGGATTACCG GTTCTTAGAA AATCTGATAG ATGTTGACAC TATCCAAAAT 
TTTTTTAAAT TACCGGAACC TGTTCAAAAT GTTACTGATT TGCAATCCGA TACTGTATTA 
TTAGTAAATC CACAGTCAAT ATATGAACAG TATGCTTTTG AGGGATTTGT CAATTCTGAA 
TTTATGATTC CTGCTAAAAA GAAAGATAAG GCCCCTTCTG CCTTATGTAA GAAACTTCCT 
TTACGATTAA ATTATTCACT TTGGGGCAAT AGAGCTAAAT CCATTCCCAA ATCACAGCAA 
GTGCGCAGTT TTTATATCAA TGACCTCTAT ATTCTCCCAG TCTCTAGACA TTTGAAAAAC 
AGCGCCTTGC TAATACCCTC CATACTGTAC CATATTGAAA ACTTATTGGT CGCCTCTTCT 
TTTATCGAAC ACTTTCGACT TGATTGTAAA ATTGACACTG CTTGTCAGGC TTTAACATCT 
GCGGAATCAC AATTGAATTT TGATTACGAT CGTCTAGAGT TTTACGGAGA CTGCTTTCTA 
AAATTGGGTG CTTCTATTAC AGTTTTTTTG AAATTTCCTG ATACTCAAGA GTACCAACTG 
CATTTTAATC GAAAGAAAAT TATTAGCAAC TGTAATTTGT ATAAAGTAGC AATAGATTGT 
GAGTTGCCGA AGTATGCTCT CTCGACTCCC TTGGAAATCC GTCATTGGTG TCCATATGGT 
TTTCAGAAAA GCACATCGGA TAAGTGCCGC TACGCCGTTT TACAGAAATT ATCGGTTAAG 
AGGATAGCAG ATATGGTCGA AGCTAGTATC GGTGCATGTC TTTTAGACAG TGGACTTGAC 
TCAGCACTCA AGATCTGTAA ATCTTTAAGC GTTGGTCTGC TGGATATCAG CAATTGGGAT 
GAGTGGAACA ATTATTTTGA TTTAAATACA TATGCGGATT CACTGAGAAA TGTTCAATTC 
CCTTACTCCT CGTATATAGA GGAAACTATT GGATATTCAT TTAAAAACAA GAAACTACTC 
CATTTGGCAT TTATTCATCC TTCCATGATG TCTCAGCAAG GTATTTACGA AAACTATCAA 
CAGTTGGAGT TTTTGGGTGA TGCTGTATTG GATTACATTA TCGTACAATA CCTTTATAAA 
AAGTATCCTA ACGCAACTTC TGGCGAATTA ACTGATTACA AATCTTTTTA TGTGTGTAAC 
AAGAGTCTAT CATACATTGG CTTTGTTTTG AATTTGCACA AATATATCCA ACATGAAAGC 
GCAGCAATGT GTGATGCAAT ATTTGAATAT CAAGAATTAA TTGAAGCGTT CAGGGAGACT 
GCTTCAGAGA ATCCGTGGTT CTGGTTTGAA ATTGATTCAC CAAAGTTCAT TTCAGATACT 
TTAGAAGCTA TGATATGTGC CATTTTTTTG GATTCTGGGT TTAGTTTACA ATCTCTACAA 
TTCGTTTTAC CTCTTTTTCT TAATTCGTTA GGGGATGCGA CACATACTAA GGCTAAAGGA 
GATATTGAAC ACAAGGTATA CCAATTACTG AAAGATCAGG GATGTGAAGA CTTCGGAACA 
AAGTGTGTCA TCGAGGAGGT GAAATCCAGT CACAAAACAT TGTTAAATAC TGAACTCCAT 
TTAACAAAGT ATTATGGGTT TTCATTCTTC CGCCACGGGA ATATTGTTGC TTACGGCAAA 
TCCCGTAAAG TTGCCAATGC AAAGTATATT ATGAAACAAA GACTTCTCAA ATTGTTAGAG 
GATAAGTCTA ACTTACTTTT GTATTCTTGT AATTGCAAAT TTAGTAAGAA AAAGCCATCA 
GATGAGCAAA TAAAAGGAGA TGGAAAAGTT AAAAGTTTGA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_588215.2
CDS	285..4409
Translation	MDISSFLLPQLLRKYQQDVYNIASKQNTLLVMRTGAGKTLLAVKLIKQKLEEQILIQESNLEHKKISVFLVNKVPLVFQQAEYIRSQLPAKVGMFYGELSIEMSEQLLTNIILKYNVIVITADLFYLFLARGFLSINDLNLIIFDECHHAIGNDAYARIMNDFYHRAKAVLSKKHFTLPRIFGMTASPFTGKKGNLYHRLYQWEQLFDSKAHVVSENELADYFCLPEESYVMYSNKLVVPPSDSIIKKCEETLQGCKLISRAVKTALAETIDMGLWFGEQVWLYLVDFVETKRLKKKALGKQLSDDEELAIDRLKIFVEDWKNNKYSDNGPRIPVFDSTDVTDKVFKLLELLKATYRKSDSVRTVIFVERKATAFTLSLFMKTLNLPNIRAHSFIGHGPSDQGEFSMTFRRQKDTLHKFKTGKYNVLIATAVAEEGIDVPSCNLVIRFNICRTVTQYVQSRGRARAMASKFLIFLNTEELLIHERILHEEKNLKFALSELSNSNIFDSLVCEERERVTDDIVYEVGETGALLTGLYAVSLLYNFCNTLSRDVYTRYYPTFTAQPCLSGWYCFEVELPKACKVPAAQGSPAKSIRKAKQNAAFIMCLDLIRMGLIDKHLKPLDFRRKIADLETLEEDELKDEGYIETYERYVPKSWMKVPEDITRCFVSLLYTDANEGDNHIFHPLVFVQAHSFPKIDSFILNSTVGPRVKIVLETIEDSFKIDSHLLELLKKSTRYLLQFGLSTSLEQQIPTPYWLAPLNLSCTDYRFLENLIDVDTIQNFFKLPEPVQNVTDLQSDTVLLVNPQSIYEQYAFEGFVNSEFMIPAKKKDKAPSALCKKLPLRLNYSLWGNRAKSIPKSQQVRSFYINDLYILPVSRHLKNSALLIPSILYHIENLLVASSFIEHFRLDCKIDTACQALTSAESQLNFDYDRLEFYGDCFLKLGASITVFLKFPDTQEYQLHFNRKKIISNCNLYKVAIDCELPKYALSTPLEIRHWCPYGFQKSTSDKCRYAVLQKLSVKRIADMVEASIGACLLDSGLDSALKICKSLSVGLLDISNWDEWNNYFDLNTYADSLRNVQFPYSSYIEETIGYSFKNKKLLHLAFIHPSMMSQQGIYENYQQLEFLGDAVLDYIIVQYLYKKYPNATSGELTDYKSFYVCNKSLSYIGFVLNLHKYIQHESAAMCDAIFEYQELIEAFRETASENPWFWFEIDSPKFISDTLEAMICAIFLDSGFSLQSLQFVLPLFLNSLGDATHTKAKGDIEHKVYQLLKDQGCEDFGTKCVIEEVKSSHKTLLNTELHLTKYYGFSFFRHGNIVAYGKSRKVANAKYIMKQRLLKLLEDKSNLLLYSCNCKFSKKKPSDEQIKGDGKVKSLT

Target ORF information:

RefSeq Version	NM_001023205.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe dicer (dcr1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001023205.2

ORF Insert Sequence:

ATGGATATTT CAAGTTTTCT ACTTCCTCAA CTTTTACGTA AATATCAACA AGATGTGTAT 
AATATCGCGA GCAAGCAAAA TACTTTACTT GTTATGAGAA CGGGCGCTGG TAAGACATTA 
CTTGCTGTGA AGTTGATAAA ACAAAAGCTC GAGGAGCAAA TTTTAATCCA AGAATCAAAT 
CTTGAACATA AAAAAATATC AGTTTTTCTC GTCAACAAAG TCCCTTTGGT ATTTCAACAA 
GCGGAATACA TTCGATCTCA ACTACCGGCT AAGGTTGGCA TGTTTTATGG CGAATTATCT 
ATAGAAATGA GCGAGCAGTT GTTGACTAAT ATTATATTGA AGTATAATGT GATTGTTATT 
ACTGCAGATT TGTTCTATTT GTTTCTTGCA AGAGGTTTTC TTTCAATAAA TGATTTGAAT 
TTAATTATAT TCGACGAATG TCATCATGCA ATTGGAAATG ATGCGTATGC TCGCATCATG 
AATGATTTTT ATCACAGAGC CAAAGCAGTA TTGTCAAAAA AACATTTCAC CCTACCAAGA 
ATTTTTGGTA TGACTGCTTC ACCATTCACT GGAAAAAAAG GAAACTTATA CCATCGACTG 
TATCAATGGG AGCAATTATT TGATTCTAAA GCACACGTGG TTTCGGAAAA CGAGCTAGCC 
GATTACTTCT GTCTTCCCGA AGAAAGCTAT GTAATGTATT CCAATAAGTT GGTTGTGCCA 
CCCTCGGATT CTATTATCAA GAAATGCGAG GAAACTCTTC AAGGATGCAA GTTAATTTCT 
CGGGCTGTTA AGACTGCTTT AGCAGAAACC ATAGATATGG GTCTTTGGTT TGGGGAGCAA 
GTTTGGTTAT ATTTGGTTGA TTTTGTGGAA ACGAAAAGAT TAAAAAAAAA GGCTTTAGGG 
AAGCAGTTGT CAGATGACGA GGAACTGGCA ATTGACCGGT TAAAAATATT TGTTGAAGAT 
TGGAAAAATA ACAAATATTC AGACAATGGC CCTAGAATCC CTGTTTTTGA TTCCACTGAT 
GTTACTGATA AAGTCTTTAA ACTCTTAGAA TTGTTAAAGG CTACTTACCG CAAAAGTGAT 
AGCGTTCGTA CGGTTATTTT CGTTGAAAGA AAAGCTACGG CGTTTACTTT AAGTTTGTTT 
ATGAAAACTC TTAATCTGCC TAACATCCGC GCTCATTCTT TTATAGGACA TGGACCGTCC 
GATCAGGGTG AATTTTCTAT GACATTCAGG AGGCAAAAAG ATACCCTTCA TAAGTTTAAG 
ACTGGAAAAT ATAATGTTTT AATTGCTACT GCAGTTGCAG AAGAAGGTAT CGATGTACCA 
TCATGTAACT TAGTTATACG CTTCAATATT TGTCGGACTG TCACCCAGTA TGTCCAATCT 
CGAGGTAGAG CGAGAGCAAT GGCTTCAAAG TTTCTAATTT TTTTAAACAC AGAAGAGTTG 
TTAATTCATG AACGCATTCT ACACGAAGAA AAAAATCTTA AATTTGCCCT TTCAGAGCTC 
AGCAATTCGA ATATTTTTGA TTCATTGGTA TGTGAGGAAA GAGAACGTGT GACTGATGAT 
ATCGTCTATG AAGTTGGCGA GACTGGTGCT TTACTCACAG GGTTGTATGC AGTTAGTCTG 
CTTTATAACT TTTGTAACAC ACTTTCAAGA GACGTATACA CAAGATATTA TCCCACTTTT 
ACAGCTCAAC CCTGTCTTTC AGGTTGGTAT TGTTTTGAGG TAGAATTGCC AAAAGCCTGC 
AAAGTTCCAG CGGCTCAAGG ATCTCCCGCT AAATCAATTA GGAAAGCCAA ACAGAATGCT 
GCGTTCATCA TGTGTTTGGA TCTGATTCGT ATGGGTCTTA TAGACAAACA TTTAAAACCC 
CTAGATTTTA GAAGAAAAAT TGCCGACCTT GAAACTCTTG AGGAAGACGA GCTAAAAGAT 
GAAGGTTATA TCGAGACATA TGAGCGCTAT GTACCAAAAA GTTGGATGAA AGTTCCTGAA 
GATATTACAC GTTGCTTCGT CTCTTTACTT TATACTGATG CTAATGAAGG AGACAATCAT 
ATATTCCATC CCTTAGTGTT TGTACAAGCT CATTCATTCC CCAAAATTGA TAGCTTTATT 
CTTAATTCGA CTGTTGGCCC CCGAGTTAAA ATTGTTTTAG AAACGATTGA GGATAGTTTT 
AAGATCGATT CTCATCTGCT TGAGTTGTTA AAAAAATCAA CTCGTTATCT ACTTCAATTC 
GGTTTATCTA CTTCTCTTGA GCAACAAATA CCTACTCCTT ACTGGCTTGC GCCTTTAAAT 
TTGTCATGCA CGGATTACCG GTTCTTAGAA AATCTGATAG ATGTTGACAC TATCCAAAAT 
TTTTTTAAAT TACCGGAACC TGTTCAAAAT GTTACTGATT TGCAATCCGA TACTGTATTA 
TTAGTAAATC CACAGTCAAT ATATGAACAG TATGCTTTTG AGGGATTTGT CAATTCTGAA 
TTTATGATTC CTGCTAAAAA GAAAGATAAG GCCCCTTCTG CCTTATGTAA GAAACTTCCT 
TTACGATTAA ATTATTCACT TTGGGGCAAT AGAGCTAAAT CCATTCCCAA ATCACAGCAA 
GTGCGCAGTT TTTATATCAA TGACCTCTAT ATTCTCCCAG TCTCTAGACA TTTGAAAAAC 
AGCGCCTTGC TAATACCCTC CATACTGTAC CATATTGAAA ACTTATTGGT CGCCTCTTCT 
TTTATCGAAC ACTTTCGACT TGATTGTAAA ATTGACACTG CTTGTCAGGC TTTAACATCT 
GCGGAATCAC AATTGAATTT TGATTACGAT CGTCTAGAGT TTTACGGAGA CTGCTTTCTA 
AAATTGGGTG CTTCTATTAC AGTTTTTTTG AAATTTCCTG ATACTCAAGA GTACCAACTG 
CATTTTAATC GAAAGAAAAT TATTAGCAAC TGTAATTTGT ATAAAGTAGC AATAGATTGT 
GAGTTGCCGA AGTATGCTCT CTCGACTCCC TTGGAAATCC GTCATTGGTG TCCATATGGT 
TTTCAGAAAA GCACATCGGA TAAGTGCCGC TACGCCGTTT TACAGAAATT ATCGGTTAAG 
AGGATAGCAG ATATGGTCGA AGCTAGTATC GGTGCATGTC TTTTAGACAG TGGACTTGAC 
TCAGCACTCA AGATCTGTAA ATCTTTAAGC GTTGGTCTGC TGGATATCAG CAATTGGGAT 
GAGTGGAACA ATTATTTTGA TTTAAATACA TATGCGGATT CACTGAGAAA TGTTCAATTC 
CCTTACTCCT CGTATATAGA GGAAACTATT GGATATTCAT TTAAAAACAA GAAACTACTC 
CATTTGGCAT TTATTCATCC TTCCATGATG TCTCAGCAAG GTATTTACGA AAACTATCAA 
CAGTTGGAGT TTTTGGGTGA TGCTGTATTG GATTACATTA TCGTACAATA CCTTTATAAA 
AAGTATCCTA ACGCAACTTC TGGCGAATTA ACTGATTACA AATCTTTTTA TGTGTGTAAC 
AAGAGTCTAT CATACATTGG CTTTGTTTTG AATTTGCACA AATATATCCA ACATGAAAGC 
GCAGCAATGT GTGATGCAAT ATTTGAATAT CAAGAATTAA TTGAAGCGTT CAGGGAGACT 
GCTTCAGAGA ATCCGTGGTT CTGGTTTGAA ATTGATTCAC CAAAGTTCAT TTCAGATACT 
TTAGAAGCTA TGATATGTGC CATTTTTTTG GATTCTGGGT TTAGTTTACA ATCTCTACAA 
TTCGTTTTAC CTCTTTTTCT TAATTCGTTA GGGGATGCGA CACATACTAA GGCTAAAGGA 
GATATTGAAC ACAAGGTATA CCAATTACTG AAAGATCAGG GATGTGAAGA CTTCGGAACA 
AAGTGTGTCA TCGAGGAGGT GAAATCCAGT CACAAAACAT TGTTAAATAC TGAACTCCAT 
TTAACAAAGT ATTATGGGTT TTCATTCTTC CGCCACGGGA ATATTGTTGC TTACGGCAAA 
TCCCGTAAAG TTGCCAATGC AAAGTATATT ATGAAACAAA GACTTCTCAA ATTGTTAGAG 
GATAAGTCTA ACTTACTTTT GTATTCTTGT AATTGCAAAT TTAGTAAGAA AAAGCCATCA 
GATGAGCAAA TAAAAGGAGA TGGAAAAGTT AAAAGTTTGA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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dcr1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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