RAD8 cDNA ORF clone, Schizosaccharomyces pombe 972h-

Gene
Clones
gRNAs

Summary

Gene Symbol	RAD8
Entrez Gene ID	3361391
Full Name	ubiquitin-protein ligase E3 Rad8
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe 972h-

mRNA and Protein(s)

mRNA	Protein	Name
NM_001355968.1	NP_001342793.1	ubiquitin-protein ligase E3 Rad8
XM_001712982.2	XP_001713034.1	ubiquitin-protein ligase E3 Rad8

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0527100	NP_001047001.1
Kluyveromyces lactis	KLLA0F17479g	XP_455865.1
Schizosaccharomyces pombe (fission yeast)	rad8	XP_001713034.1
Eremothecium gossypii	AGOS_AFR220W	NP_985767.2
Arabidopsis thaliana (thale cress)	RAD5	NP_197667.1
Magnaporthe oryzae (rice blast fungus)	MGG_05032	XP_003712540.1
Neurospora crassa	NCU09516	XP_958511.1
Saccharomyces cerevisiae (baker's yeast)	RAD5	NP_013132.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response
Title: Essential domains of Schizosaccharomyces pombe Rad8 required for DNA damage response.

The following RAD8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAD8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101724	NM_001355968.1 Latest version!	Schizosaccharomyces pombe ubiquitin-protein ligase E3 Rad8 (rad8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OSj04880	XM_001712982.2 Latest version!	Schizosaccharomyces pombe 972h- ubiquitin-protein ligase E3 Rad8 (rad8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAD8 ( NM_001355968.1 ) cDNA ORF clone, Schizosaccharomyces pombe 972h- -> NP_001342793.1 Schizosaccharomyces pombe ubiquitin-protein ligase E3 Rad8 (rad8), mRNA.

Price & Availability↑

CloneID	OSc101724
Clone ID Related Accession (Same CDS sequence)	NM_001355968.1
Accession Version	NM_001355968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	ubiquitin-protein ligase E3 Rad8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424).

ATGAAAAGAA AGGTACAAAA AATTATAGAT GAAGCCCCTT TAGAAGAAAA TTCTCCTCCT 
CGATTTTTTG ATTCAGATGT TGAAGCTGAC TCTAAACCGA ATGATTTAAC TGCTGCGAAT 
TCCATCGTAG ACTTAAAAAC AAATTCTCAA CATGAAAATG CTAACGCAGC AGGCAAGGAA 
TATGGAGATA GTGGAGTTTC AGAGTCATGG GTTTTAGACT TTTTATCTGT CACTGGTGAA 
AAAACAATTT CGGAATTTTT AGCTCAAAAA ATTTGGAAAA CCTCGAATGG AGATTTGAAT 
GTCGCCGTTG ATATGTATTT TGATGAATCC TTTAATATAA AGAATTCGAA TCCTGACAGT 
GAAAGTCAGA AAGACACTGA TGCCTCGTTG ACCCAGATGG ACCAATTATC TAATACTGTA 
TCCGTTAAGG ATCTTAGTAT TAATAGGAAC ACAAATAAAA AAGCCTTAAA TGCTGTTAGC 
CCTTCATTGA ATTTGTCATC TAACAGTTCT GTTCAAGATG TATCTATTGA TAAAGAAGAA 
ATGATGAAGA AGCAATCACG GAATGCTCTT ACTCCGCTGG ACTTTATTAT GAAGAAGAAT 
GAGTTGATGA AGTATATCGG ATGCTTTGGT GTGGAGGCTT ATTCAACTGC ATCAGGTACT 
CGAACACTCC AGGCTGGCGA AAGGATCTAT TTAGAACGTC AAAAACTTTC CATAAAATCA 
CAATCGAGAA ATTCTAGGAA AAAATCAAAA TTGCTAAGCA TTAATTCTTC ATGCTATAGC 
AATATCGTTA GATTTTGTAA TTCAGATCAT CATGAAATTG GTAAATTACC TACTGAAGTC 
GCTTCTGTTA TTTCAACGTT GATGGAGCAA GGTTTTTGGT CATTTGAAGC CATATGCATT 
TACTCCGACA ATATAATTAG ATTTGGGAGT AATGTCACTT TACAAGTTTA TTGTTTTATC 
AATGTCAATC ATCCGTCCTT GAACCGATCT CCATTCACTT TGGCAACTAA TTCTATGCAA 
GAAGAGGAAG AACATTTAAA AGCCTCTTTT GCTCAAAATA AACGTGATCA TTTACTTCGT 
TTGTTTACTT GGATAGCTTT GGAACCAGAC CTTGAAGACT GCAACACTAA AGAGAGTATT 
CATATTGATG ATATATTGAA AACAAGTTCA TTGCCTGAAG CGAGGGACGA GAGTAACTCA 
GATCTAACCC CATCCTCAAC TGAAGACGAG GAAGATGTTG TTAGCGACCA ACTTGCTATA 
TTATATGACA AAGTTAAAAC TTCTGGAGCT GAATTGCCGT CTGCTCCAAA GCCATCAACG 
TTTGCTCTTG ATCTTCGTGA GTATCAAAAA CAGGCTTTGT ATTGGATGTG TTGTAAAGAA 
GAGGGCGTAC AAAGTGATGG TAGTGCACCT AAGCTTCATC CTTTGTGGAG TCGATTTCGA 
TTTCCAAAGG ACTCAGAATT CCCTGAATTC TTTAAATGTT CTAGTGATGA TGACAACACA 
CATTTTTACG TTAACTTATA TACTGGCGAA ACTACAATGT TGTTTCCGAA TTCAATGCCT 
TATCATCGGG GTGGAATTTT AGCAGACGAA ATGGGCTTAG GCAAAACGAT TGAAGTATTA 
TCACTTATTC ATTCTAGACC TTGCTTTTCA ACCGATGAAA TACCAGAAGC CTTTAGACAT 
TCTAAACCCA GCCTTCCTGT CGCCAGTAGG ACGACTTTGG TAGTAGCACC TATGTCATTG 
TTAGATCAAT GGCACTCCGA AGCTTGTAAG GTGTCTCAAG GCACCAAATT CAGAAGCATG 
ATTTATTATG GTTCTGAAAA GCCTTTGGAT CTGAAGTCTT GTGTTATTGA TACATCCACA 
GCTCCCTTGA TCATCATAAC TAGTTATGGT GTGTTACTTT CAGAATTTAG TCAGCAATCT 
CATTCTTCGG GATTGTTTTC TGTGCATTGG TTCCGTGTTG TCCTTGATGA AGGACATAAC 
ATTAGAAATA GGGAAAGCAA AACCGCGAAA GCGTGTCATA GTATTTCGTC CCAGAATCGT 
TGGGTGATTA CAGGTACGCC AATAGTCAAT AAACTGGATG ACTTGTATAG CTTGATTAAA 
TTTATGCGAT ATGAGCCGTG GTGTAATTAC ACTTATTGGC AAACCTTTGT GAGTCTGCCA 
TATCAGTCAA AAGATGTCTT AAAGGCGTTG AATGTTGTTC AATCAATTTT GGAGTTTTTA 
GTTTTGAGAA GAACCAAAGA AACTAAAGAT AGAAACGGAA ACTCAATTGT TACGTTACCT 
CCAAAAACCG TAAAAATTGA ATATTTGGAC TTTTCTGACT CAGAAAGGAA AATTTATGAC 
TCTCTCTATA CCAAAGCAAA AAGTACCGTT AATGCAAACA TAGTTGCCGG AACGCTGTTC 
AGAAACTATA CAACGATCCT CGGATTACTT TTAAGATTAA GACAGGCGTG TTGTGACCCT 
GTGCTTCTTT CCAATATGAC CATTAACAGT GAAACGTTTG ATGATTTTGA GTTTTCGGTT 
GAGCAATTTA ATTCTTTAAT TAATCAATTT GTTGTCACTG GAAAGCCCAT TCCTTCAGAT 
ATTTTAAAAA TTGATACACT GAAGTCTTTT GAAGCATTGA TAACGGAATG TCCCATATGT 
TGTAATGAAC CAATTCAAAA TCCCTTGTTA TTGAACTGCA AGCATGCTTG TTGCGGAGAT 
TGTTTGTCAG AACATATTCA GTATCAAAAA AGAAGAAACA TAATTCCTCC GCTTTGTCAT 
ACATGTCGTC AACCCTTCAA TGAGCAAGAT GTCTATAAAC CTTTTTTTGT CAAAAATAAC 
GGAACACAGT CTACGCTTTT AGTCGGTGAG GAAGTAAAAT GGAAATATTG GAATAGGCTA 
CAATCAGTGA AACTTAATGG CCTTTTAGGA CAATTGCGTC AATTAACTCA TAGTTCTGAA 
CCTGAAAAGG TCGTTATCTT TTCACAGTTT ACCACATTCC TGGATATTAT CGCAGACGTT 
CTCGAGTCAG AGAAGATGGG GTATGCTAGA TTCGATGGTA CCATGTCACA ACAAATGAGG 
TCGACGGCTT TAGAGACATT TCGAAATGAT CCTGATGTTA ACGTGCTGAT TATCTCTTTA 
AAGGCAGGCG GGGTTGGTTT AAACCTTACT TGTGCCAACC ATGTTTTTAT TATGGATCCA 
TGGTGGAGCT GGTCAGTGGA AGCACAGGCC ATCGATCGTA TTCATCGTTT AGGACAGGAA 
AAGCCTGTAT TTGTGACGAG ATATATTGTA CGTGATACTG TTGAGGAAAG GATGCTCAAA 
ATACAAGAAC GCAAAAATTT TATTACTGGG ACCCTGGGCA TGTCGGAAGG AAAGCAGCAG 
GTTCAGTCAA TTGAAGACAT CAAAATGTTG TTTGAATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342793.1
CDS	329..3730
Translation	MKRKVQKIIDEAPLEENSPPRFFDSDVEADSKPNDLTAANSIVDLKTNSQHENANAAGKEYGDSGVSESWVLDFLSVTGEKTISEFLAQKIWKTSNGDLNVAVDMYFDESFNIKNSNPDSESQKDTDASLTQMDQLSNTVSVKDLSINRNTNKKALNAVSPSLNLSSNSSVQDVSIDKEEMMKKQSRNALTPLDFIMKKNELMKYIGCFGVEAYSTASGTRTLQAGERIYLERQKLSIKSQSRNSRKKSKLLSINSSCYSNIVRFCNSDHHEIGKLPTEVASVISTLMEQGFWSFEAICIYSDNIIRFGSNVTLQVYCFINVNHPSLNRSPFTLATNSMQEEEEHLKASFAQNKRDHLLRLFTWIALEPDLEDCNTKESIHIDDILKTSSLPEARDESNSDLTPSSTEDEEDVVSDQLAILYDKVKTSGAELPSAPKPSTFALDLREYQKQALYWMCCKEEGVQSDGSAPKLHPLWSRFRFPKDSEFPEFFKCSSDDDNTHFYVNLYTGETTMLFPNSMPYHRGGILADEMGLGKTIEVLSLIHSRPCFSTDEIPEAFRHSKPSLPVASRTTLVVAPMSLLDQWHSEACKVSQGTKFRSMIYYGSEKPLDLKSCVIDTSTAPLIIITSYGVLLSEFSQQSHSSGLFSVHWFRVVLDEGHNIRNRESKTAKACHSISSQNRWVITGTPIVNKLDDLYSLIKFMRYEPWCNYTYWQTFVSLPYQSKDVLKALNVVQSILEFLVLRRTKETKDRNGNSIVTLPPKTVKIEYLDFSDSERKIYDSLYTKAKSTVNANIVAGTLFRNYTTILGLLLRLRQACCDPVLLSNMTINSETFDDFEFSVEQFNSLINQFVVTGKPIPSDILKIDTLKSFEALITECPICCNEPIQNPLLLNCKHACCGDCLSEHIQYQKRRNIIPPLCHTCRQPFNEQDVYKPFFVKNNGTQSTLLVGEEVKWKYWNRLQSVKLNGLLGQLRQLTHSSEPEKVVIFSQFTTFLDIIADVLESEKMGYARFDGTMSQQMRSTALETFRNDPDVNVLIISLKAGGVGLNLTCANHVFIMDPWWSWSVEAQAIDRIHRLGQEKPVFVTRYIVRDTVEERMLKIQERKNFITGTLGMSEGKQQVQSIEDIKMLFEY

Target ORF information:

RefSeq Version	NM_001355968.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe ubiquitin-protein ligase E3 Rad8 (rad8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355968.1

ORF Insert Sequence:

ATGAAAAGAA AGGTACAAAA AATTATAGAT GAAGCCCCTT TAGAAGAAAA TTCTCCTCCT 
CGATTTTTTG ATTCAGATGT TGAAGCTGAC TCTAAACCGA ATGATTTAAC TGCTGCGAAT 
TCCATCGTAG ACTTAAAAAC AAATTCTCAA CATGAAAATG CTAACGCAGC AGGCAAGGAA 
TATGGAGATA GTGGAGTTTC AGAGTCATGG GTTTTAGACT TTTTATCTGT CACTGGTGAA 
AAAACAATTT CGGAATTTTT AGCTCAAAAA ATTTGGAAAA CCTCGAATGG AGATTTGAAT 
GTCGCCGTTG ATATGTATTT TGATGAATCC TTTAATATAA AGAATTCGAA TCCTGACAGT 
GAAAGTCAGA AAGACACTGA TGCCTCGTTG ACCCAGATGG ACCAATTATC TAATACTGTA 
TCCGTTAAGG ATCTTAGTAT TAATAGGAAC ACAAATAAAA AAGCCTTAAA TGCTGTTAGC 
CCTTCATTGA ATTTGTCATC TAACAGTTCT GTTCAAGATG TATCTATTGA TAAAGAAGAA 
ATGATGAAGA AGCAATCACG GAATGCTCTT ACTCCGCTGG ACTTTATTAT GAAGAAGAAT 
GAGTTGATGA AGTATATCGG ATGCTTTGGT GTGGAGGCTT ATTCAACTGC ATCAGGTACT 
CGAACACTCC AGGCTGGCGA AAGGATCTAT TTAGAACGTC AAAAACTTTC CATAAAATCA 
CAATCGAGAA ATTCTAGGAA AAAATCAAAA TTGCTAAGCA TTAATTCTTC ATGCTATAGC 
AATATCGTTA GATTTTGTAA TTCAGATCAT CATGAAATTG GTAAATTACC TACTGAAGTC 
GCTTCTGTTA TTTCAACGTT GATGGAGCAA GGTTTTTGGT CATTTGAAGC CATATGCATT 
TACTCCGACA ATATAATTAG ATTTGGGAGT AATGTCACTT TACAAGTTTA TTGTTTTATC 
AATGTCAATC ATCCGTCCTT GAACCGATCT CCATTCACTT TGGCAACTAA TTCTATGCAA 
GAAGAGGAAG AACATTTAAA AGCCTCTTTT GCTCAAAATA AACGTGATCA TTTACTTCGT 
TTGTTTACTT GGATAGCTTT GGAACCAGAC CTTGAAGACT GCAACACTAA AGAGAGTATT 
CATATTGATG ATATATTGAA AACAAGTTCA TTGCCTGAAG CGAGGGACGA GAGTAACTCA 
GATCTAACCC CATCCTCAAC TGAAGACGAG GAAGATGTTG TTAGCGACCA ACTTGCTATA 
TTATATGACA AAGTTAAAAC TTCTGGAGCT GAATTGCCGT CTGCTCCAAA GCCATCAACG 
TTTGCTCTTG ATCTTCGTGA GTATCAAAAA CAGGCTTTGT ATTGGATGTG TTGTAAAGAA 
GAGGGCGTAC AAAGTGATGG TAGTGCACCT AAGCTTCATC CTTTGTGGAG TCGATTTCGA 
TTTCCAAAGG ACTCAGAATT CCCTGAATTC TTTAAATGTT CTAGTGATGA TGACAACACA 
CATTTTTACG TTAACTTATA TACTGGCGAA ACTACAATGT TGTTTCCGAA TTCAATGCCT 
TATCATCGGG GTGGAATTTT AGCAGACGAA ATGGGCTTAG GCAAAACGAT TGAAGTATTA 
TCACTTATTC ATTCTAGACC TTGCTTTTCA ACCGATGAAA TACCAGAAGC CTTTAGACAT 
TCTAAACCCA GCCTTCCTGT CGCCAGTAGG ACGACTTTGG TAGTAGCACC TATGTCATTG 
TTAGATCAAT GGCACTCCGA AGCTTGTAAG GTGTCTCAAG GCACCAAATT CAGAAGCATG 
ATTTATTATG GTTCTGAAAA GCCTTTGGAT CTGAAGTCTT GTGTTATTGA TACATCCACA 
GCTCCCTTGA TCATCATAAC TAGTTATGGT GTGTTACTTT CAGAATTTAG TCAGCAATCT 
CATTCTTCGG GATTGTTTTC TGTGCATTGG TTCCGTGTTG TCCTTGATGA AGGACATAAC 
ATTAGAAATA GGGAAAGCAA AACCGCGAAA GCGTGTCATA GTATTTCGTC CCAGAATCGT 
TGGGTGATTA CAGGTACGCC AATAGTCAAT AAACTGGATG ACTTGTATAG CTTGATTAAA 
TTTATGCGAT ATGAGCCGTG GTGTAATTAC ACTTATTGGC AAACCTTTGT GAGTCTGCCA 
TATCAGTCAA AAGATGTCTT AAAGGCGTTG AATGTTGTTC AATCAATTTT GGAGTTTTTA 
GTTTTGAGAA GAACCAAAGA AACTAAAGAT AGAAACGGAA ACTCAATTGT TACGTTACCT 
CCAAAAACCG TAAAAATTGA ATATTTGGAC TTTTCTGACT CAGAAAGGAA AATTTATGAC 
TCTCTCTATA CCAAAGCAAA AAGTACCGTT AATGCAAACA TAGTTGCCGG AACGCTGTTC 
AGAAACTATA CAACGATCCT CGGATTACTT TTAAGATTAA GACAGGCGTG TTGTGACCCT 
GTGCTTCTTT CCAATATGAC CATTAACAGT GAAACGTTTG ATGATTTTGA GTTTTCGGTT 
GAGCAATTTA ATTCTTTAAT TAATCAATTT GTTGTCACTG GAAAGCCCAT TCCTTCAGAT 
ATTTTAAAAA TTGATACACT GAAGTCTTTT GAAGCATTGA TAACGGAATG TCCCATATGT 
TGTAATGAAC CAATTCAAAA TCCCTTGTTA TTGAACTGCA AGCATGCTTG TTGCGGAGAT 
TGTTTGTCAG AACATATTCA GTATCAAAAA AGAAGAAACA TAATTCCTCC GCTTTGTCAT 
ACATGTCGTC AACCCTTCAA TGAGCAAGAT GTCTATAAAC CTTTTTTTGT CAAAAATAAC 
GGAACACAGT CTACGCTTTT AGTCGGTGAG GAAGTAAAAT GGAAATATTG GAATAGGCTA 
CAATCAGTGA AACTTAATGG CCTTTTAGGA CAATTGCGTC AATTAACTCA TAGTTCTGAA 
CCTGAAAAGG TCGTTATCTT TTCACAGTTT ACCACATTCC TGGATATTAT CGCAGACGTT 
CTCGAGTCAG AGAAGATGGG GTATGCTAGA TTCGATGGTA CCATGTCACA ACAAATGAGG 
TCGACGGCTT TAGAGACATT TCGAAATGAT CCTGATGTTA ACGTGCTGAT TATCTCTTTA 
AAGGCAGGCG GGGTTGGTTT AAACCTTACT TGTGCCAACC ATGTTTTTAT TATGGATCCA 
TGGTGGAGCT GGTCAGTGGA AGCACAGGCC ATCGATCGTA TTCATCGTTT AGGACAGGAA 
AAGCCTGTAT TTGTGACGAG ATATATTGTA CGTGATACTG TTGAGGAAAG GATGCTCAAA 
ATACAAGAAC GCAAAAATTT TATTACTGGG ACCCTGGGCA TGTCGGAAGG AAAGCAGCAG 
GTTCAGTCAA TTGAAGACAT CAAAATGTTG TTTGAATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAD8 ( XM_001712982.2 ) cDNA ORF clone, Schizosaccharomyces pombe 972h- -> XP_001713034.1 Schizosaccharomyces pombe 972h- ubiquitin-protein ligase E3 Rad8 (rad8), mRNA.

Price & Availability↑

CloneID	OSj04880
Clone ID Related Accession (Same CDS sequence)	XM_001712982.2
Accession Version	XM_001712982.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3402bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	ubiquitin-protein ligase E3 Rad8
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced XM_001712982.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGAAAAGAA AGGTACAAAA AATTATAGAT GAAGCCCCTT TAGAAGAAAA TTCTCCTCCT 
CGATTTTTTG ATTCAGATGT TGAAGCTGAC TCTAAACCGA ATGATTTAAC TGCTGCGAAT 
TCCATCGTAG ACTTAAAAAC AAATTCTCAA CATGAAAATG CTAACGCAGC AGGCAAGGAA 
TATGGAGATA GTGGAGTTTC AGAGTCATGG GTTTTAGACT TTTTATCTGT CACTGGTGAA 
AAAACAATTT CGGAATTTTT AGCTCAAAAA ATTTGGAAAA CCTCGAATGG AGATTTGAAT 
GTCGCCGTTG ATATGTATTT TGATGAATCC TTTAATATAA AGAATTCGAA TCCTGACAGT 
GAAAGTCAGA AAGACACTGA TGCCTCGTTG ACCCAGATGG ACCAATTATC TAATACTGTA 
TCCGTTAAGG ATCTTAGTAT TAATAGGAAC ACAAATAAAA AAGCCTTAAA TGCTGTTAGC 
CCTTCATTGA ATTTGTCATC TAACAGTTCT GTTCAAGATG TATCTATTGA TAAAGAAGAA 
ATGATGAAGA AGCAATCACG GAATGCTCTT ACTCCGCTGG ACTTTATTAT GAAGAAGAAT 
GAGTTGATGA AGTATATCGG ATGCTTTGGT GTGGAGGCTT ATTCAACTGC ATCAGGTACT 
CGAACACTCC AGGCTGGCGA AAGGATCTAT TTAGAACGTC AAAAACTTTC CATAAAATCA 
CAATCGAGAA ATTCTAGGAA AAAATCAAAA TTGCTAAGCA TTAATTCTTC ATGCTATAGC 
AATATCGTTA GATTTTGTAA TTCAGATCAT CATGAAATTG GTAAATTACC TACTGAAGTC 
GCTTCTGTTA TTTCAACGTT GATGGAGCAA GGTTTTTGGT CATTTGAAGC CATATGCATT 
TACTCCGACA ATATAATTAG ATTTGGGAGT AATGTCACTT TACAAGTTTA TTGTTTTATC 
AATGTCAATC ATCCGTCCTT GAACCGATCT CCATTCACTT TGGCAACTAA TTCTATGCAA 
GAAGAGGAAG AACATTTAAA AGCCTCTTTT GCTCAAAATA AACGTGATCA TTTACTTCGT 
TTGTTTACTT GGATAGCTTT GGAACCAGAC CTTGAAGACT GCAACACTAA AGAGAGTATT 
CATATTGATG ATATATTGAA AACAAGTTCA TTGCCTGAAG CGAGGGACGA GAGTAACTCA 
GATCTAACCC CATCCTCAAC TGAAGACGAG GAAGATGTTG TTAGCGACCA ACTTGCTATA 
TTATATGACA AAGTTAAAAC TTCTGGAGCT GAATTGCCGT CTGCTCCAAA GCCATCAACG 
TTTGCTCTTG ATCTTCGTGA GTATCAAAAA CAGGCTTTGT ATTGGATGTG TTGTAAAGAA 
GAGGGCGTAC AAAGTGATGG TAGTGCACCT AAGCTTCATC CTTTGTGGAG TCGATTTCGA 
TTTCCAAAGG ACTCAGAATT CCCTGAATTC TTTAAATGTT CTAGTGATGA TGACAACACA 
CATTTTTACG TTAACTTATA TACTGGCGAA ACTACAATGT TGTTTCCGAA TTCAATGCCT 
TATCATCGGG GTGGAATTTT AGCAGACGAA ATGGGCTTAG GCAAAACGAT TGAAGTATTA 
TCACTTATTC ATTCTAGACC TTGCTTTTCA ACCGATGAAA TACCAGAAGC CTTTAGACAT 
TCTAAACCCA GCCTTCCTGT CGCCAGTAGG ACGACTTTGG TAGTAGCACC TATGTCATTG 
TTAGATCAAT GGCACTCCGA AGCTTGTAAG GTGTCTCAAG GCACCAAATT CAGAAGCATG 
ATTTATTATG GTTCTGAAAA GCCTTTGGAT CTGAAGTCTT GTGTTATTGA TACATCCACA 
GCTCCCTTGA TCATCATAAC TAGTTATGGT GTGTTACTTT CAGAATTTAG TCAGCAATCT 
CATTCTTCGG GATTGTTTTC TGTGCATTGG TTCCGTGTTG TCCTTGATGA AGGACATAAC 
ATTAGAAATA GGGAAAGCAA AACCGCGAAA GCGTGTCATA GTATTTCGTC CCAGAATCGT 
TGGGTGATTA CAGGTACGCC AATAGTCAAT AAACTGGATG ACTTGTATAG CTTGATTAAA 
TTTATGCGAT ATGAGCCGTG GTGTAATTAC ACTTATTGGC AAACCTTTGT GAGTCTGCCA 
TATCAGTCAA AAGATGTCTT AAAGGCGTTG AATGTTGTTC AATCAATTTT GGAGTTTTTA 
GTTTTGAGAA GAACCAAAGA AACTAAAGAT AGAAACGGAA ACTCAATTGT TACGTTACCT 
CCAAAAACCG TAAAAATTGA ATATTTGGAC TTTTCTGACT CAGAAAGGAA AATTTATGAC 
TCTCTCTATA CCAAAGCAAA AAGTACCGTT AATGCAAACA TAGTTGCCGG AACGCTGTTC 
AGAAACTATA CAACGATCCT CGGATTACTT TTAAGATTAA GACAGGCGTG TTGTGACCCT 
GTGCTTCTTT CCAATATGAC CATTAACAGT GAAACGTTTG ATGATTTTGA GTTTTCGGTT 
GAGCAATTTA ATTCTTTAAT TAATCAATTT GTTGTCACTG GAAAGCCCAT TCCTTCAGAT 
ATTTTAAAAA TTGATACACT GAAGTCTTTT GAAGCATTGA TAACGGAATG TCCCATATGT 
TGTAATGAAC CAATTCAAAA TCCCTTGTTA TTGAACTGCA AGCATGCTTG TTGCGGAGAT 
TGTTTGTCAG AACATATTCA GTATCAAAAA AGAAGAAACA TAATTCCTCC GCTTTGTCAT 
ACATGTCGTC AACCCTTCAA TGAGCAAGAT GTCTATAAAC CTTTTTTTGT CAAAAATAAC 
GGAACACAGT CTACGCTTTT AGTCGGTGAG GAAGTAAAAT GGAAATATTG GAATAGGCTA 
CAATCAGTGA AACTTAATGG CCTTTTAGGA CAATTGCGTC AATTAACTCA TAGTTCTGAA 
CCTGAAAAGG TCGTTATCTT TTCACAGTTT ACCACATTCC TGGATATTAT CGCAGACGTT 
CTCGAGTCAG AGAAGATGGG GTATGCTAGA TTCGATGGTA CCATGTCACA ACAAATGAGG 
TCGACGGCTT TAGAGACATT TCGAAATGAT CCTGATGTTA ACGTGCTGAT TATCTCTTTA 
AAGGCAGGCG GGGTTGGTTT AAACCTTACT TGTGCCAACC ATGTTTTTAT TATGGATCCA 
TGGTGGAGCT GGTCAGTGGA AGCACAGGCC ATCGATCGTA TTCATCGTTT AGGACAGGAA 
AAGCCTGTAT TTGTGACGAG ATATATTGTA CGTGATACTG TTGAGGAAAG GATGCTCAAA 
ATACAAGAAC GCAAAAATTT TATTACTGGG ACCCTGGGCA TGTCGGAAGG AAAGCAGCAG 
GTTCAGTCAA TTGAAGACAT CAAAATGTTG TTTGAATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001713034.1
CDS	329..3730
Translation	MKRKVQKIIDEAPLEENSPPRFFDSDVEADSKPNDLTAANSIVDLKTNSQHENANAAGKEYGDSGVSESWVLDFLSVTGEKTISEFLAQKIWKTSNGDLNVAVDMYFDESFNIKNSNPDSESQKDTDASLTQMDQLSNTVSVKDLSINRNTNKKALNAVSPSLNLSSNSSVQDVSIDKEEMMKKQSRNALTPLDFIMKKNELMKYIGCFGVEAYSTASGTRTLQAGERIYLERQKLSIKSQSRNSRKKSKLLSINSSCYSNIVRFCNSDHHEIGKLPTEVASVISTLMEQGFWSFEAICIYSDNIIRFGSNVTLQVYCFINVNHPSLNRSPFTLATNSMQEEEEHLKASFAQNKRDHLLRLFTWIALEPDLEDCNTKESIHIDDILKTSSLPEARDESNSDLTPSSTEDEEDVVSDQLAILYDKVKTSGAELPSAPKPSTFALDLREYQKQALYWMCCKEEGVQSDGSAPKLHPLWSRFRFPKDSEFPEFFKCSSDDDNTHFYVNLYTGETTMLFPNSMPYHRGGILADEMGLGKTIEVLSLIHSRPCFSTDEIPEAFRHSKPSLPVASRTTLVVAPMSLLDQWHSEACKVSQGTKFRSMIYYGSEKPLDLKSCVIDTSTAPLIIITSYGVLLSEFSQQSHSSGLFSVHWFRVVLDEGHNIRNRESKTAKACHSISSQNRWVITGTPIVNKLDDLYSLIKFMRYEPWCNYTYWQTFVSLPYQSKDVLKALNVVQSILEFLVLRRTKETKDRNGNSIVTLPPKTVKIEYLDFSDSERKIYDSLYTKAKSTVNANIVAGTLFRNYTTILGLLLRLRQACCDPVLLSNMTINSETFDDFEFSVEQFNSLINQFVVTGKPIPSDILKIDTLKSFEALITECPICCNEPIQNPLLLNCKHACCGDCLSEHIQYQKRRNIIPPLCHTCRQPFNEQDVYKPFFVKNNGTQSTLLVGEEVKWKYWNRLQSVKLNGLLGQLRQLTHSSEPEKVVIFSQFTTFLDIIADVLESEKMGYARFDGTMSQQMRSTALETFRNDPDVNVLIISLKAGGVGLNLTCANHVFIMDPWWSWSVEAQAIDRIHRLGQEKPVFVTRYIVRDTVEERMLKIQERKNFITGTLGMSEGKQQVQSIEDIKMLFEY

Target ORF information:

RefSeq Version	XM_001712982.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- ubiquitin-protein ligase E3 Rad8 (rad8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001712982.2

ORF Insert Sequence:

ATGAAAAGAA AGGTACAAAA AATTATAGAT GAAGCCCCTT TAGAAGAAAA TTCTCCTCCT 
CGATTTTTTG ATTCAGATGT TGAAGCTGAC TCTAAACCGA ATGATTTAAC TGCTGCGAAT 
TCCATCGTAG ACTTAAAAAC AAATTCTCAA CATGAAAATG CTAACGCAGC AGGCAAGGAA 
TATGGAGATA GTGGAGTTTC AGAGTCATGG GTTTTAGACT TTTTATCTGT CACTGGTGAA 
AAAACAATTT CGGAATTTTT AGCTCAAAAA ATTTGGAAAA CCTCGAATGG AGATTTGAAT 
GTCGCCGTTG ATATGTATTT TGATGAATCC TTTAATATAA AGAATTCGAA TCCTGACAGT 
GAAAGTCAGA AAGACACTGA TGCCTCGTTG ACCCAGATGG ACCAATTATC TAATACTGTA 
TCCGTTAAGG ATCTTAGTAT TAATAGGAAC ACAAATAAAA AAGCCTTAAA TGCTGTTAGC 
CCTTCATTGA ATTTGTCATC TAACAGTTCT GTTCAAGATG TATCTATTGA TAAAGAAGAA 
ATGATGAAGA AGCAATCACG GAATGCTCTT ACTCCGCTGG ACTTTATTAT GAAGAAGAAT 
GAGTTGATGA AGTATATCGG ATGCTTTGGT GTGGAGGCTT ATTCAACTGC ATCAGGTACT 
CGAACACTCC AGGCTGGCGA AAGGATCTAT TTAGAACGTC AAAAACTTTC CATAAAATCA 
CAATCGAGAA ATTCTAGGAA AAAATCAAAA TTGCTAAGCA TTAATTCTTC ATGCTATAGC 
AATATCGTTA GATTTTGTAA TTCAGATCAT CATGAAATTG GTAAATTACC TACTGAAGTC 
GCTTCTGTTA TTTCAACGTT GATGGAGCAA GGTTTTTGGT CATTTGAAGC CATATGCATT 
TACTCCGACA ATATAATTAG ATTTGGGAGT AATGTCACTT TACAAGTTTA TTGTTTTATC 
AATGTCAATC ATCCGTCCTT GAACCGATCT CCATTCACTT TGGCAACTAA TTCTATGCAA 
GAAGAGGAAG AACATTTAAA AGCCTCTTTT GCTCAAAATA AACGTGATCA TTTACTTCGT 
TTGTTTACTT GGATAGCTTT GGAACCAGAC CTTGAAGACT GCAACACTAA AGAGAGTATT 
CATATTGATG ATATATTGAA AACAAGTTCA TTGCCTGAAG CGAGGGACGA GAGTAACTCA 
GATCTAACCC CATCCTCAAC TGAAGACGAG GAAGATGTTG TTAGCGACCA ACTTGCTATA 
TTATATGACA AAGTTAAAAC TTCTGGAGCT GAATTGCCGT CTGCTCCAAA GCCATCAACG 
TTTGCTCTTG ATCTTCGTGA GTATCAAAAA CAGGCTTTGT ATTGGATGTG TTGTAAAGAA 
GAGGGCGTAC AAAGTGATGG TAGTGCACCT AAGCTTCATC CTTTGTGGAG TCGATTTCGA 
TTTCCAAAGG ACTCAGAATT CCCTGAATTC TTTAAATGTT CTAGTGATGA TGACAACACA 
CATTTTTACG TTAACTTATA TACTGGCGAA ACTACAATGT TGTTTCCGAA TTCAATGCCT 
TATCATCGGG GTGGAATTTT AGCAGACGAA ATGGGCTTAG GCAAAACGAT TGAAGTATTA 
TCACTTATTC ATTCTAGACC TTGCTTTTCA ACCGATGAAA TACCAGAAGC CTTTAGACAT 
TCTAAACCCA GCCTTCCTGT CGCCAGTAGG ACGACTTTGG TAGTAGCACC TATGTCATTG 
TTAGATCAAT GGCACTCCGA AGCTTGTAAG GTGTCTCAAG GCACCAAATT CAGAAGCATG 
ATTTATTATG GTTCTGAAAA GCCTTTGGAT CTGAAGTCTT GTGTTATTGA TACATCCACA 
GCTCCCTTGA TCATCATAAC TAGTTATGGT GTGTTACTTT CAGAATTTAG TCAGCAATCT 
CATTCTTCGG GATTGTTTTC TGTGCATTGG TTCCGTGTTG TCCTTGATGA AGGACATAAC 
ATTAGAAATA GGGAAAGCAA AACCGCGAAA GCGTGTCATA GTATTTCGTC CCAGAATCGT 
TGGGTGATTA CAGGTACGCC AATAGTCAAT AAACTGGATG ACTTGTATAG CTTGATTAAA 
TTTATGCGAT ATGAGCCGTG GTGTAATTAC ACTTATTGGC AAACCTTTGT GAGTCTGCCA 
TATCAGTCAA AAGATGTCTT AAAGGCGTTG AATGTTGTTC AATCAATTTT GGAGTTTTTA 
GTTTTGAGAA GAACCAAAGA AACTAAAGAT AGAAACGGAA ACTCAATTGT TACGTTACCT 
CCAAAAACCG TAAAAATTGA ATATTTGGAC TTTTCTGACT CAGAAAGGAA AATTTATGAC 
TCTCTCTATA CCAAAGCAAA AAGTACCGTT AATGCAAACA TAGTTGCCGG AACGCTGTTC 
AGAAACTATA CAACGATCCT CGGATTACTT TTAAGATTAA GACAGGCGTG TTGTGACCCT 
GTGCTTCTTT CCAATATGAC CATTAACAGT GAAACGTTTG ATGATTTTGA GTTTTCGGTT 
GAGCAATTTA ATTCTTTAAT TAATCAATTT GTTGTCACTG GAAAGCCCAT TCCTTCAGAT 
ATTTTAAAAA TTGATACACT GAAGTCTTTT GAAGCATTGA TAACGGAATG TCCCATATGT 
TGTAATGAAC CAATTCAAAA TCCCTTGTTA TTGAACTGCA AGCATGCTTG TTGCGGAGAT 
TGTTTGTCAG AACATATTCA GTATCAAAAA AGAAGAAACA TAATTCCTCC GCTTTGTCAT 
ACATGTCGTC AACCCTTCAA TGAGCAAGAT GTCTATAAAC CTTTTTTTGT CAAAAATAAC 
GGAACACAGT CTACGCTTTT AGTCGGTGAG GAAGTAAAAT GGAAATATTG GAATAGGCTA 
CAATCAGTGA AACTTAATGG CCTTTTAGGA CAATTGCGTC AATTAACTCA TAGTTCTGAA 
CCTGAAAAGG TCGTTATCTT TTCACAGTTT ACCACATTCC TGGATATTAT CGCAGACGTT 
CTCGAGTCAG AGAAGATGGG GTATGCTAGA TTCGATGGTA CCATGTCACA ACAAATGAGG 
TCGACGGCTT TAGAGACATT TCGAAATGAT CCTGATGTTA ACGTGCTGAT TATCTCTTTA 
AAGGCAGGCG GGGTTGGTTT AAACCTTACT TGTGCCAACC ATGTTTTTAT TATGGATCCA 
TGGTGGAGCT GGTCAGTGGA AGCACAGGCC ATCGATCGTA TTCATCGTTT AGGACAGGAA 
AAGCCTGTAT TTGTGACGAG ATATATTGTA CGTGATACTG TTGAGGAAAG GATGCTCAAA 
ATACAAGAAC GCAAAAATTT TATTACTGGG ACCCTGGGCA TGTCGGAAGG AAAGCAGCAG 
GTTCAGTCAA TTGAAGACAT CAAAATGTTG TTTGAATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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RAD8 cDNA ORF clone, Schizosaccharomyces pombe 972h-

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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