RAD8 cDNA ORF clone, Schizosaccharomyces pombe 972h-

The following RAD8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAD8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc101724 NM_001355968.1
Latest version!
Schizosaccharomyces pombe ubiquitin-protein ligase E3 Rad8 (rad8), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OSj04880 XM_001712982.2
Latest version!
Schizosaccharomyces pombe 972h- ubiquitin-protein ligase E3 Rad8 (rad8), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc101724
    Clone ID Related Accession (Same CDS sequence) NM_001355968.1
    Accession Version NM_001355968.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product ubiquitin-protein ligase E3 Rad8
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAAAGAA AGGTACAAAA AATTATAGAT GAAGCCCCTT TAGAAGAAAA TTCTCCTCCT 
    CGATTTTTTG ATTCAGATGT TGAAGCTGAC TCTAAACCGA ATGATTTAAC TGCTGCGAAT
    TCCATCGTAG ACTTAAAAAC AAATTCTCAA CATGAAAATG CTAACGCAGC AGGCAAGGAA
    TATGGAGATA GTGGAGTTTC AGAGTCATGG GTTTTAGACT TTTTATCTGT CACTGGTGAA
    AAAACAATTT CGGAATTTTT AGCTCAAAAA ATTTGGAAAA CCTCGAATGG AGATTTGAAT
    GTCGCCGTTG ATATGTATTT TGATGAATCC TTTAATATAA AGAATTCGAA TCCTGACAGT
    GAAAGTCAGA AAGACACTGA TGCCTCGTTG ACCCAGATGG ACCAATTATC TAATACTGTA
    TCCGTTAAGG ATCTTAGTAT TAATAGGAAC ACAAATAAAA AAGCCTTAAA TGCTGTTAGC
    CCTTCATTGA ATTTGTCATC TAACAGTTCT GTTCAAGATG TATCTATTGA TAAAGAAGAA
    ATGATGAAGA AGCAATCACG GAATGCTCTT ACTCCGCTGG ACTTTATTAT GAAGAAGAAT
    GAGTTGATGA AGTATATCGG ATGCTTTGGT GTGGAGGCTT ATTCAACTGC ATCAGGTACT
    CGAACACTCC AGGCTGGCGA AAGGATCTAT TTAGAACGTC AAAAACTTTC CATAAAATCA
    CAATCGAGAA ATTCTAGGAA AAAATCAAAA TTGCTAAGCA TTAATTCTTC ATGCTATAGC
    AATATCGTTA GATTTTGTAA TTCAGATCAT CATGAAATTG GTAAATTACC TACTGAAGTC
    GCTTCTGTTA TTTCAACGTT GATGGAGCAA GGTTTTTGGT CATTTGAAGC CATATGCATT
    TACTCCGACA ATATAATTAG ATTTGGGAGT AATGTCACTT TACAAGTTTA TTGTTTTATC
    AATGTCAATC ATCCGTCCTT GAACCGATCT CCATTCACTT TGGCAACTAA TTCTATGCAA
    GAAGAGGAAG AACATTTAAA AGCCTCTTTT GCTCAAAATA AACGTGATCA TTTACTTCGT
    TTGTTTACTT GGATAGCTTT GGAACCAGAC CTTGAAGACT GCAACACTAA AGAGAGTATT
    CATATTGATG ATATATTGAA AACAAGTTCA TTGCCTGAAG CGAGGGACGA GAGTAACTCA
    GATCTAACCC CATCCTCAAC TGAAGACGAG GAAGATGTTG TTAGCGACCA ACTTGCTATA
    TTATATGACA AAGTTAAAAC TTCTGGAGCT GAATTGCCGT CTGCTCCAAA GCCATCAACG
    TTTGCTCTTG ATCTTCGTGA GTATCAAAAA CAGGCTTTGT ATTGGATGTG TTGTAAAGAA
    GAGGGCGTAC AAAGTGATGG TAGTGCACCT AAGCTTCATC CTTTGTGGAG TCGATTTCGA
    TTTCCAAAGG ACTCAGAATT CCCTGAATTC TTTAAATGTT CTAGTGATGA TGACAACACA
    CATTTTTACG TTAACTTATA TACTGGCGAA ACTACAATGT TGTTTCCGAA TTCAATGCCT
    TATCATCGGG GTGGAATTTT AGCAGACGAA ATGGGCTTAG GCAAAACGAT TGAAGTATTA
    TCACTTATTC ATTCTAGACC TTGCTTTTCA ACCGATGAAA TACCAGAAGC CTTTAGACAT
    TCTAAACCCA GCCTTCCTGT CGCCAGTAGG ACGACTTTGG TAGTAGCACC TATGTCATTG
    TTAGATCAAT GGCACTCCGA AGCTTGTAAG GTGTCTCAAG GCACCAAATT CAGAAGCATG
    ATTTATTATG GTTCTGAAAA GCCTTTGGAT CTGAAGTCTT GTGTTATTGA TACATCCACA
    GCTCCCTTGA TCATCATAAC TAGTTATGGT GTGTTACTTT CAGAATTTAG TCAGCAATCT
    CATTCTTCGG GATTGTTTTC TGTGCATTGG TTCCGTGTTG TCCTTGATGA AGGACATAAC
    ATTAGAAATA GGGAAAGCAA AACCGCGAAA GCGTGTCATA GTATTTCGTC CCAGAATCGT
    TGGGTGATTA CAGGTACGCC AATAGTCAAT AAACTGGATG ACTTGTATAG CTTGATTAAA
    TTTATGCGAT ATGAGCCGTG GTGTAATTAC ACTTATTGGC AAACCTTTGT GAGTCTGCCA
    TATCAGTCAA AAGATGTCTT AAAGGCGTTG AATGTTGTTC AATCAATTTT GGAGTTTTTA
    GTTTTGAGAA GAACCAAAGA AACTAAAGAT AGAAACGGAA ACTCAATTGT TACGTTACCT
    CCAAAAACCG TAAAAATTGA ATATTTGGAC TTTTCTGACT CAGAAAGGAA AATTTATGAC
    TCTCTCTATA CCAAAGCAAA AAGTACCGTT AATGCAAACA TAGTTGCCGG AACGCTGTTC
    AGAAACTATA CAACGATCCT CGGATTACTT TTAAGATTAA GACAGGCGTG TTGTGACCCT
    GTGCTTCTTT CCAATATGAC CATTAACAGT GAAACGTTTG ATGATTTTGA GTTTTCGGTT
    GAGCAATTTA ATTCTTTAAT TAATCAATTT GTTGTCACTG GAAAGCCCAT TCCTTCAGAT
    ATTTTAAAAA TTGATACACT GAAGTCTTTT GAAGCATTGA TAACGGAATG TCCCATATGT
    TGTAATGAAC CAATTCAAAA TCCCTTGTTA TTGAACTGCA AGCATGCTTG TTGCGGAGAT
    TGTTTGTCAG AACATATTCA GTATCAAAAA AGAAGAAACA TAATTCCTCC GCTTTGTCAT
    ACATGTCGTC AACCCTTCAA TGAGCAAGAT GTCTATAAAC CTTTTTTTGT CAAAAATAAC
    GGAACACAGT CTACGCTTTT AGTCGGTGAG GAAGTAAAAT GGAAATATTG GAATAGGCTA
    CAATCAGTGA AACTTAATGG CCTTTTAGGA CAATTGCGTC AATTAACTCA TAGTTCTGAA
    CCTGAAAAGG TCGTTATCTT TTCACAGTTT ACCACATTCC TGGATATTAT CGCAGACGTT
    CTCGAGTCAG AGAAGATGGG GTATGCTAGA TTCGATGGTA CCATGTCACA ACAAATGAGG
    TCGACGGCTT TAGAGACATT TCGAAATGAT CCTGATGTTA ACGTGCTGAT TATCTCTTTA
    AAGGCAGGCG GGGTTGGTTT AAACCTTACT TGTGCCAACC ATGTTTTTAT TATGGATCCA
    TGGTGGAGCT GGTCAGTGGA AGCACAGGCC ATCGATCGTA TTCATCGTTT AGGACAGGAA
    AAGCCTGTAT TTGTGACGAG ATATATTGTA CGTGATACTG TTGAGGAAAG GATGCTCAAA
    ATACAAGAAC GCAAAAATTT TATTACTGGG ACCCTGGGCA TGTCGGAAGG AAAGCAGCAG
    GTTCAGTCAA TTGAAGACAT CAAAATGTTG TTTGAATATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001342793.1
    CDS329..3730
    Translation

    Target ORF information:

    RefSeq Version NM_001355968.1
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe ubiquitin-protein ligase E3 Rad8 (rad8), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001355968.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAAAGAA AGGTACAAAA AATTATAGAT GAAGCCCCTT TAGAAGAAAA TTCTCCTCCT 
    CGATTTTTTG ATTCAGATGT TGAAGCTGAC TCTAAACCGA ATGATTTAAC TGCTGCGAAT
    TCCATCGTAG ACTTAAAAAC AAATTCTCAA CATGAAAATG CTAACGCAGC AGGCAAGGAA
    TATGGAGATA GTGGAGTTTC AGAGTCATGG GTTTTAGACT TTTTATCTGT CACTGGTGAA
    AAAACAATTT CGGAATTTTT AGCTCAAAAA ATTTGGAAAA CCTCGAATGG AGATTTGAAT
    GTCGCCGTTG ATATGTATTT TGATGAATCC TTTAATATAA AGAATTCGAA TCCTGACAGT
    GAAAGTCAGA AAGACACTGA TGCCTCGTTG ACCCAGATGG ACCAATTATC TAATACTGTA
    TCCGTTAAGG ATCTTAGTAT TAATAGGAAC ACAAATAAAA AAGCCTTAAA TGCTGTTAGC
    CCTTCATTGA ATTTGTCATC TAACAGTTCT GTTCAAGATG TATCTATTGA TAAAGAAGAA
    ATGATGAAGA AGCAATCACG GAATGCTCTT ACTCCGCTGG ACTTTATTAT GAAGAAGAAT
    GAGTTGATGA AGTATATCGG ATGCTTTGGT GTGGAGGCTT ATTCAACTGC ATCAGGTACT
    CGAACACTCC AGGCTGGCGA AAGGATCTAT TTAGAACGTC AAAAACTTTC CATAAAATCA
    CAATCGAGAA ATTCTAGGAA AAAATCAAAA TTGCTAAGCA TTAATTCTTC ATGCTATAGC
    AATATCGTTA GATTTTGTAA TTCAGATCAT CATGAAATTG GTAAATTACC TACTGAAGTC
    GCTTCTGTTA TTTCAACGTT GATGGAGCAA GGTTTTTGGT CATTTGAAGC CATATGCATT
    TACTCCGACA ATATAATTAG ATTTGGGAGT AATGTCACTT TACAAGTTTA TTGTTTTATC
    AATGTCAATC ATCCGTCCTT GAACCGATCT CCATTCACTT TGGCAACTAA TTCTATGCAA
    GAAGAGGAAG AACATTTAAA AGCCTCTTTT GCTCAAAATA AACGTGATCA TTTACTTCGT
    TTGTTTACTT GGATAGCTTT GGAACCAGAC CTTGAAGACT GCAACACTAA AGAGAGTATT
    CATATTGATG ATATATTGAA AACAAGTTCA TTGCCTGAAG CGAGGGACGA GAGTAACTCA
    GATCTAACCC CATCCTCAAC TGAAGACGAG GAAGATGTTG TTAGCGACCA ACTTGCTATA
    TTATATGACA AAGTTAAAAC TTCTGGAGCT GAATTGCCGT CTGCTCCAAA GCCATCAACG
    TTTGCTCTTG ATCTTCGTGA GTATCAAAAA CAGGCTTTGT ATTGGATGTG TTGTAAAGAA
    GAGGGCGTAC AAAGTGATGG TAGTGCACCT AAGCTTCATC CTTTGTGGAG TCGATTTCGA
    TTTCCAAAGG ACTCAGAATT CCCTGAATTC TTTAAATGTT CTAGTGATGA TGACAACACA
    CATTTTTACG TTAACTTATA TACTGGCGAA ACTACAATGT TGTTTCCGAA TTCAATGCCT
    TATCATCGGG GTGGAATTTT AGCAGACGAA ATGGGCTTAG GCAAAACGAT TGAAGTATTA
    TCACTTATTC ATTCTAGACC TTGCTTTTCA ACCGATGAAA TACCAGAAGC CTTTAGACAT
    TCTAAACCCA GCCTTCCTGT CGCCAGTAGG ACGACTTTGG TAGTAGCACC TATGTCATTG
    TTAGATCAAT GGCACTCCGA AGCTTGTAAG GTGTCTCAAG GCACCAAATT CAGAAGCATG
    ATTTATTATG GTTCTGAAAA GCCTTTGGAT CTGAAGTCTT GTGTTATTGA TACATCCACA
    GCTCCCTTGA TCATCATAAC TAGTTATGGT GTGTTACTTT CAGAATTTAG TCAGCAATCT
    CATTCTTCGG GATTGTTTTC TGTGCATTGG TTCCGTGTTG TCCTTGATGA AGGACATAAC
    ATTAGAAATA GGGAAAGCAA AACCGCGAAA GCGTGTCATA GTATTTCGTC CCAGAATCGT
    TGGGTGATTA CAGGTACGCC AATAGTCAAT AAACTGGATG ACTTGTATAG CTTGATTAAA
    TTTATGCGAT ATGAGCCGTG GTGTAATTAC ACTTATTGGC AAACCTTTGT GAGTCTGCCA
    TATCAGTCAA AAGATGTCTT AAAGGCGTTG AATGTTGTTC AATCAATTTT GGAGTTTTTA
    GTTTTGAGAA GAACCAAAGA AACTAAAGAT AGAAACGGAA ACTCAATTGT TACGTTACCT
    CCAAAAACCG TAAAAATTGA ATATTTGGAC TTTTCTGACT CAGAAAGGAA AATTTATGAC
    TCTCTCTATA CCAAAGCAAA AAGTACCGTT AATGCAAACA TAGTTGCCGG AACGCTGTTC
    AGAAACTATA CAACGATCCT CGGATTACTT TTAAGATTAA GACAGGCGTG TTGTGACCCT
    GTGCTTCTTT CCAATATGAC CATTAACAGT GAAACGTTTG ATGATTTTGA GTTTTCGGTT
    GAGCAATTTA ATTCTTTAAT TAATCAATTT GTTGTCACTG GAAAGCCCAT TCCTTCAGAT
    ATTTTAAAAA TTGATACACT GAAGTCTTTT GAAGCATTGA TAACGGAATG TCCCATATGT
    TGTAATGAAC CAATTCAAAA TCCCTTGTTA TTGAACTGCA AGCATGCTTG TTGCGGAGAT
    TGTTTGTCAG AACATATTCA GTATCAAAAA AGAAGAAACA TAATTCCTCC GCTTTGTCAT
    ACATGTCGTC AACCCTTCAA TGAGCAAGAT GTCTATAAAC CTTTTTTTGT CAAAAATAAC
    GGAACACAGT CTACGCTTTT AGTCGGTGAG GAAGTAAAAT GGAAATATTG GAATAGGCTA
    CAATCAGTGA AACTTAATGG CCTTTTAGGA CAATTGCGTC AATTAACTCA TAGTTCTGAA
    CCTGAAAAGG TCGTTATCTT TTCACAGTTT ACCACATTCC TGGATATTAT CGCAGACGTT
    CTCGAGTCAG AGAAGATGGG GTATGCTAGA TTCGATGGTA CCATGTCACA ACAAATGAGG
    TCGACGGCTT TAGAGACATT TCGAAATGAT CCTGATGTTA ACGTGCTGAT TATCTCTTTA
    AAGGCAGGCG GGGTTGGTTT AAACCTTACT TGTGCCAACC ATGTTTTTAT TATGGATCCA
    TGGTGGAGCT GGTCAGTGGA AGCACAGGCC ATCGATCGTA TTCATCGTTT AGGACAGGAA
    AAGCCTGTAT TTGTGACGAG ATATATTGTA CGTGATACTG TTGAGGAAAG GATGCTCAAA
    ATACAAGAAC GCAAAAATTT TATTACTGGG ACCCTGGGCA TGTCGGAAGG AAAGCAGCAG
    GTTCAGTCAA TTGAAGACAT CAAAATGTTG TTTGAATATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OSj04880
    Clone ID Related Accession (Same CDS sequence) XM_001712982.2
    Accession Version XM_001712982.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2013-04-30
    Organism Schizosaccharomyces pombe 972h-
    Product ubiquitin-protein ligase E3 Rad8
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced XM_001712982.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAAAGAA AGGTACAAAA AATTATAGAT GAAGCCCCTT TAGAAGAAAA TTCTCCTCCT 
    CGATTTTTTG ATTCAGATGT TGAAGCTGAC TCTAAACCGA ATGATTTAAC TGCTGCGAAT
    TCCATCGTAG ACTTAAAAAC AAATTCTCAA CATGAAAATG CTAACGCAGC AGGCAAGGAA
    TATGGAGATA GTGGAGTTTC AGAGTCATGG GTTTTAGACT TTTTATCTGT CACTGGTGAA
    AAAACAATTT CGGAATTTTT AGCTCAAAAA ATTTGGAAAA CCTCGAATGG AGATTTGAAT
    GTCGCCGTTG ATATGTATTT TGATGAATCC TTTAATATAA AGAATTCGAA TCCTGACAGT
    GAAAGTCAGA AAGACACTGA TGCCTCGTTG ACCCAGATGG ACCAATTATC TAATACTGTA
    TCCGTTAAGG ATCTTAGTAT TAATAGGAAC ACAAATAAAA AAGCCTTAAA TGCTGTTAGC
    CCTTCATTGA ATTTGTCATC TAACAGTTCT GTTCAAGATG TATCTATTGA TAAAGAAGAA
    ATGATGAAGA AGCAATCACG GAATGCTCTT ACTCCGCTGG ACTTTATTAT GAAGAAGAAT
    GAGTTGATGA AGTATATCGG ATGCTTTGGT GTGGAGGCTT ATTCAACTGC ATCAGGTACT
    CGAACACTCC AGGCTGGCGA AAGGATCTAT TTAGAACGTC AAAAACTTTC CATAAAATCA
    CAATCGAGAA ATTCTAGGAA AAAATCAAAA TTGCTAAGCA TTAATTCTTC ATGCTATAGC
    AATATCGTTA GATTTTGTAA TTCAGATCAT CATGAAATTG GTAAATTACC TACTGAAGTC
    GCTTCTGTTA TTTCAACGTT GATGGAGCAA GGTTTTTGGT CATTTGAAGC CATATGCATT
    TACTCCGACA ATATAATTAG ATTTGGGAGT AATGTCACTT TACAAGTTTA TTGTTTTATC
    AATGTCAATC ATCCGTCCTT GAACCGATCT CCATTCACTT TGGCAACTAA TTCTATGCAA
    GAAGAGGAAG AACATTTAAA AGCCTCTTTT GCTCAAAATA AACGTGATCA TTTACTTCGT
    TTGTTTACTT GGATAGCTTT GGAACCAGAC CTTGAAGACT GCAACACTAA AGAGAGTATT
    CATATTGATG ATATATTGAA AACAAGTTCA TTGCCTGAAG CGAGGGACGA GAGTAACTCA
    GATCTAACCC CATCCTCAAC TGAAGACGAG GAAGATGTTG TTAGCGACCA ACTTGCTATA
    TTATATGACA AAGTTAAAAC TTCTGGAGCT GAATTGCCGT CTGCTCCAAA GCCATCAACG
    TTTGCTCTTG ATCTTCGTGA GTATCAAAAA CAGGCTTTGT ATTGGATGTG TTGTAAAGAA
    GAGGGCGTAC AAAGTGATGG TAGTGCACCT AAGCTTCATC CTTTGTGGAG TCGATTTCGA
    TTTCCAAAGG ACTCAGAATT CCCTGAATTC TTTAAATGTT CTAGTGATGA TGACAACACA
    CATTTTTACG TTAACTTATA TACTGGCGAA ACTACAATGT TGTTTCCGAA TTCAATGCCT
    TATCATCGGG GTGGAATTTT AGCAGACGAA ATGGGCTTAG GCAAAACGAT TGAAGTATTA
    TCACTTATTC ATTCTAGACC TTGCTTTTCA ACCGATGAAA TACCAGAAGC CTTTAGACAT
    TCTAAACCCA GCCTTCCTGT CGCCAGTAGG ACGACTTTGG TAGTAGCACC TATGTCATTG
    TTAGATCAAT GGCACTCCGA AGCTTGTAAG GTGTCTCAAG GCACCAAATT CAGAAGCATG
    ATTTATTATG GTTCTGAAAA GCCTTTGGAT CTGAAGTCTT GTGTTATTGA TACATCCACA
    GCTCCCTTGA TCATCATAAC TAGTTATGGT GTGTTACTTT CAGAATTTAG TCAGCAATCT
    CATTCTTCGG GATTGTTTTC TGTGCATTGG TTCCGTGTTG TCCTTGATGA AGGACATAAC
    ATTAGAAATA GGGAAAGCAA AACCGCGAAA GCGTGTCATA GTATTTCGTC CCAGAATCGT
    TGGGTGATTA CAGGTACGCC AATAGTCAAT AAACTGGATG ACTTGTATAG CTTGATTAAA
    TTTATGCGAT ATGAGCCGTG GTGTAATTAC ACTTATTGGC AAACCTTTGT GAGTCTGCCA
    TATCAGTCAA AAGATGTCTT AAAGGCGTTG AATGTTGTTC AATCAATTTT GGAGTTTTTA
    GTTTTGAGAA GAACCAAAGA AACTAAAGAT AGAAACGGAA ACTCAATTGT TACGTTACCT
    CCAAAAACCG TAAAAATTGA ATATTTGGAC TTTTCTGACT CAGAAAGGAA AATTTATGAC
    TCTCTCTATA CCAAAGCAAA AAGTACCGTT AATGCAAACA TAGTTGCCGG AACGCTGTTC
    AGAAACTATA CAACGATCCT CGGATTACTT TTAAGATTAA GACAGGCGTG TTGTGACCCT
    GTGCTTCTTT CCAATATGAC CATTAACAGT GAAACGTTTG ATGATTTTGA GTTTTCGGTT
    GAGCAATTTA ATTCTTTAAT TAATCAATTT GTTGTCACTG GAAAGCCCAT TCCTTCAGAT
    ATTTTAAAAA TTGATACACT GAAGTCTTTT GAAGCATTGA TAACGGAATG TCCCATATGT
    TGTAATGAAC CAATTCAAAA TCCCTTGTTA TTGAACTGCA AGCATGCTTG TTGCGGAGAT
    TGTTTGTCAG AACATATTCA GTATCAAAAA AGAAGAAACA TAATTCCTCC GCTTTGTCAT
    ACATGTCGTC AACCCTTCAA TGAGCAAGAT GTCTATAAAC CTTTTTTTGT CAAAAATAAC
    GGAACACAGT CTACGCTTTT AGTCGGTGAG GAAGTAAAAT GGAAATATTG GAATAGGCTA
    CAATCAGTGA AACTTAATGG CCTTTTAGGA CAATTGCGTC AATTAACTCA TAGTTCTGAA
    CCTGAAAAGG TCGTTATCTT TTCACAGTTT ACCACATTCC TGGATATTAT CGCAGACGTT
    CTCGAGTCAG AGAAGATGGG GTATGCTAGA TTCGATGGTA CCATGTCACA ACAAATGAGG
    TCGACGGCTT TAGAGACATT TCGAAATGAT CCTGATGTTA ACGTGCTGAT TATCTCTTTA
    AAGGCAGGCG GGGTTGGTTT AAACCTTACT TGTGCCAACC ATGTTTTTAT TATGGATCCA
    TGGTGGAGCT GGTCAGTGGA AGCACAGGCC ATCGATCGTA TTCATCGTTT AGGACAGGAA
    AAGCCTGTAT TTGTGACGAG ATATATTGTA CGTGATACTG TTGAGGAAAG GATGCTCAAA
    ATACAAGAAC GCAAAAATTT TATTACTGGG ACCCTGGGCA TGTCGGAAGG AAAGCAGCAG
    GTTCAGTCAA TTGAAGACAT CAAAATGTTG TTTGAATATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001713034.1
    CDS329..3730
    Translation

    Target ORF information:

    RefSeq Version XM_001712982.2
    Organism Schizosaccharomyces pombe 972h-
    Definition Schizosaccharomyces pombe 972h- ubiquitin-protein ligase E3 Rad8 (rad8), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001712982.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAAAGAA AGGTACAAAA AATTATAGAT GAAGCCCCTT TAGAAGAAAA TTCTCCTCCT 
    CGATTTTTTG ATTCAGATGT TGAAGCTGAC TCTAAACCGA ATGATTTAAC TGCTGCGAAT
    TCCATCGTAG ACTTAAAAAC AAATTCTCAA CATGAAAATG CTAACGCAGC AGGCAAGGAA
    TATGGAGATA GTGGAGTTTC AGAGTCATGG GTTTTAGACT TTTTATCTGT CACTGGTGAA
    AAAACAATTT CGGAATTTTT AGCTCAAAAA ATTTGGAAAA CCTCGAATGG AGATTTGAAT
    GTCGCCGTTG ATATGTATTT TGATGAATCC TTTAATATAA AGAATTCGAA TCCTGACAGT
    GAAAGTCAGA AAGACACTGA TGCCTCGTTG ACCCAGATGG ACCAATTATC TAATACTGTA
    TCCGTTAAGG ATCTTAGTAT TAATAGGAAC ACAAATAAAA AAGCCTTAAA TGCTGTTAGC
    CCTTCATTGA ATTTGTCATC TAACAGTTCT GTTCAAGATG TATCTATTGA TAAAGAAGAA
    ATGATGAAGA AGCAATCACG GAATGCTCTT ACTCCGCTGG ACTTTATTAT GAAGAAGAAT
    GAGTTGATGA AGTATATCGG ATGCTTTGGT GTGGAGGCTT ATTCAACTGC ATCAGGTACT
    CGAACACTCC AGGCTGGCGA AAGGATCTAT TTAGAACGTC AAAAACTTTC CATAAAATCA
    CAATCGAGAA ATTCTAGGAA AAAATCAAAA TTGCTAAGCA TTAATTCTTC ATGCTATAGC
    AATATCGTTA GATTTTGTAA TTCAGATCAT CATGAAATTG GTAAATTACC TACTGAAGTC
    GCTTCTGTTA TTTCAACGTT GATGGAGCAA GGTTTTTGGT CATTTGAAGC CATATGCATT
    TACTCCGACA ATATAATTAG ATTTGGGAGT AATGTCACTT TACAAGTTTA TTGTTTTATC
    AATGTCAATC ATCCGTCCTT GAACCGATCT CCATTCACTT TGGCAACTAA TTCTATGCAA
    GAAGAGGAAG AACATTTAAA AGCCTCTTTT GCTCAAAATA AACGTGATCA TTTACTTCGT
    TTGTTTACTT GGATAGCTTT GGAACCAGAC CTTGAAGACT GCAACACTAA AGAGAGTATT
    CATATTGATG ATATATTGAA AACAAGTTCA TTGCCTGAAG CGAGGGACGA GAGTAACTCA
    GATCTAACCC CATCCTCAAC TGAAGACGAG GAAGATGTTG TTAGCGACCA ACTTGCTATA
    TTATATGACA AAGTTAAAAC TTCTGGAGCT GAATTGCCGT CTGCTCCAAA GCCATCAACG
    TTTGCTCTTG ATCTTCGTGA GTATCAAAAA CAGGCTTTGT ATTGGATGTG TTGTAAAGAA
    GAGGGCGTAC AAAGTGATGG TAGTGCACCT AAGCTTCATC CTTTGTGGAG TCGATTTCGA
    TTTCCAAAGG ACTCAGAATT CCCTGAATTC TTTAAATGTT CTAGTGATGA TGACAACACA
    CATTTTTACG TTAACTTATA TACTGGCGAA ACTACAATGT TGTTTCCGAA TTCAATGCCT
    TATCATCGGG GTGGAATTTT AGCAGACGAA ATGGGCTTAG GCAAAACGAT TGAAGTATTA
    TCACTTATTC ATTCTAGACC TTGCTTTTCA ACCGATGAAA TACCAGAAGC CTTTAGACAT
    TCTAAACCCA GCCTTCCTGT CGCCAGTAGG ACGACTTTGG TAGTAGCACC TATGTCATTG
    TTAGATCAAT GGCACTCCGA AGCTTGTAAG GTGTCTCAAG GCACCAAATT CAGAAGCATG
    ATTTATTATG GTTCTGAAAA GCCTTTGGAT CTGAAGTCTT GTGTTATTGA TACATCCACA
    GCTCCCTTGA TCATCATAAC TAGTTATGGT GTGTTACTTT CAGAATTTAG TCAGCAATCT
    CATTCTTCGG GATTGTTTTC TGTGCATTGG TTCCGTGTTG TCCTTGATGA AGGACATAAC
    ATTAGAAATA GGGAAAGCAA AACCGCGAAA GCGTGTCATA GTATTTCGTC CCAGAATCGT
    TGGGTGATTA CAGGTACGCC AATAGTCAAT AAACTGGATG ACTTGTATAG CTTGATTAAA
    TTTATGCGAT ATGAGCCGTG GTGTAATTAC ACTTATTGGC AAACCTTTGT GAGTCTGCCA
    TATCAGTCAA AAGATGTCTT AAAGGCGTTG AATGTTGTTC AATCAATTTT GGAGTTTTTA
    GTTTTGAGAA GAACCAAAGA AACTAAAGAT AGAAACGGAA ACTCAATTGT TACGTTACCT
    CCAAAAACCG TAAAAATTGA ATATTTGGAC TTTTCTGACT CAGAAAGGAA AATTTATGAC
    TCTCTCTATA CCAAAGCAAA AAGTACCGTT AATGCAAACA TAGTTGCCGG AACGCTGTTC
    AGAAACTATA CAACGATCCT CGGATTACTT TTAAGATTAA GACAGGCGTG TTGTGACCCT
    GTGCTTCTTT CCAATATGAC CATTAACAGT GAAACGTTTG ATGATTTTGA GTTTTCGGTT
    GAGCAATTTA ATTCTTTAAT TAATCAATTT GTTGTCACTG GAAAGCCCAT TCCTTCAGAT
    ATTTTAAAAA TTGATACACT GAAGTCTTTT GAAGCATTGA TAACGGAATG TCCCATATGT
    TGTAATGAAC CAATTCAAAA TCCCTTGTTA TTGAACTGCA AGCATGCTTG TTGCGGAGAT
    TGTTTGTCAG AACATATTCA GTATCAAAAA AGAAGAAACA TAATTCCTCC GCTTTGTCAT
    ACATGTCGTC AACCCTTCAA TGAGCAAGAT GTCTATAAAC CTTTTTTTGT CAAAAATAAC
    GGAACACAGT CTACGCTTTT AGTCGGTGAG GAAGTAAAAT GGAAATATTG GAATAGGCTA
    CAATCAGTGA AACTTAATGG CCTTTTAGGA CAATTGCGTC AATTAACTCA TAGTTCTGAA
    CCTGAAAAGG TCGTTATCTT TTCACAGTTT ACCACATTCC TGGATATTAT CGCAGACGTT
    CTCGAGTCAG AGAAGATGGG GTATGCTAGA TTCGATGGTA CCATGTCACA ACAAATGAGG
    TCGACGGCTT TAGAGACATT TCGAAATGAT CCTGATGTTA ACGTGCTGAT TATCTCTTTA
    AAGGCAGGCG GGGTTGGTTT AAACCTTACT TGTGCCAACC ATGTTTTTAT TATGGATCCA
    TGGTGGAGCT GGTCAGTGGA AGCACAGGCC ATCGATCGTA TTCATCGTTT AGGACAGGAA
    AAGCCTGTAT TTGTGACGAG ATATATTGTA CGTGATACTG TTGAGGAAAG GATGCTCAAA
    ATACAAGAAC GCAAAAATTT TATTACTGGG ACCCTGGGCA TGTCGGAAGG AAAGCAGCAG
    GTTCAGTCAA TTGAAGACAT CAAAATGTTG TTTGAATATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
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