CHD1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CHD1
Entrez Gene ID	856911
Full Name	chromatin-remodeling ATPase CHD1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179054.1	NP_011091.1	chromatin-remodeling ATPase CHD1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CHD1	XP_517850.2
	CHD1	XP_001097125.1
Mus musculus (house mouse)	Chd1	NP_031716.2
Eremothecium gossypii	AGOS_AGR123C	NP_986789.1
Rattus norvegicus (Norway rat)	Chd1	NP_001100935.1
Danio rerio (zebrafish)	zgc:173506	NP_001121770.2
Saccharomyces cerevisiae (baker's yeast)	CHD1	NP_011091.1
Schizosaccharomyces pombe (fission yeast)	hrp3	NP_594967.1
Homo sapiens (human)	CHD1	NP_001261.2
Canis lupus familiaris (dog)	CHD1	XP_848459.1
Gallus gallus (chicken)	CHD1	NP_990272.1
Kluyveromyces lactis	KLLA0C17578g	XP_452985.1
Schizosaccharomyces pombe (fission yeast)	hrp1	NP_593660.1
Bos taurus (cattle)	CHD1	NP_001178977.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

It has been shown that the monomeric Chd1 remodeler shifts DNA back and forth by dynamically alternating between different segments of the nucleosome.
Title: The Chd1 Chromatin Remodeler Shifts Nucleosomal DNA Bidirectionally as a Monomer.

Intron retention was significantly lower in the absence of CHD1, suggesting that CHD1 function as a chromatin remodeler could indirectly affect RNA splicing.
Title: The ATP-dependent chromatin remodeler Chd1 is recruited by transcription elongation factors and maintains H3K4me3/H3K36me3 domains at actively transcribed and spliced genes.

Kinetic and equilibrium experiments show that poly(dA:dT) tracts perturb remodeling reactions if within one and a half helical turns of superhelix location 2 (SHL2), where the Chd1 ATPase engages nucleosomal DNA.
Title: The Sequence of Nucleosomal DNA Modulates Sliding by the Chd1 Chromatin Remodeler.

The orientation of the DNA-binding domain is mediated by sequences in the N-terminus and mutations to this part of the protein have positive and negative effects on Chd1 activity.
Title: Structural reorganization of the chromatin remodeling enzyme Chd1 upon engagement with nucleosomes.

Chd1 takes part in chromatin reorganization surrounding transcribing RNA polymerase II (Pol II) in asymmetric nucleosomes; ubiquitin-conjugated histone H2B stimulates nucleosome sliding by Chd1.
Title: The Chd1 chromatin remodeler shifts hexasomes unidirectionally.

The following CHD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05659	NM_001179054.1 Latest version!	Saccharomyces cerevisiae S288C chromatin-remodeling ATPase CHD1 (CHD1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHD1 ( NM_001179054.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011091.1 Saccharomyces cerevisiae S288C chromatin-remodeling ATPase CHD1 (CHD1), partial mRNA.

Price & Availability↑

CloneID	OSi05659
Clone ID Related Accession (Same CDS sequence)	NM_001179054.1
Accession Version	NM_001179054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	chromatin-remodeling ATPase CHD1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCAGCCA AGGATATTTC TACTGAAGTG CTGCAAAATC CTGAACTATA TGGGCTGAGG 
CGGTCACATA GAGCTGCTGC GCATCAACAA AACTACTTCA ACGATAGCGA TGATGAGGAC 
GATGAAGATA ATATTAAACA GTCGAGGAGG AAGAGAATGA CCACGATTGA AGACGACGAA 
GACGAATTTG AAGATGAAGA AGGCGAAGAA GATTCTGGAG AGGACGAAGA TGAGGAAGAT 
TTTGAAGAAG ATGATGATTA TTATGGCTCT CCTATAAAAC AAAATCGATC AAAACCGAAA 
TCAAGAACAA AATCAAAGTC TAAATCTAAG CCAAAATCTC AATCTGAAAA GCAATCTACA 
GTGAAAATAC CAACAAGGTT TTCTAATCGT CAAAACAAAA CGGTAAATTA TAACATCGAT 
TATAGCGATG ATGACCTCTT AGAATCTGAA GATGACTATG GTTCCGAGGA GGCTTTATCA 
GAGGAAAATG TGCATGAAGC ATCTGCCAAT CCTCAACCAG AGGACTTCCA CGGTATTGAT 
ATTGTTATCA ATCACAGACT AAAGACATCT TTGGAAGAAG GAAAGGTCTT GGAAAAGACT 
GTTCCTGACC TCAATAACTG CAAGGAAAAT TATGAATTCT TAATCAAATG GACAGATGAA 
TCTCATCTCC ACAATACCTG GGAAACTTAT GAATCCATTG GCCAAGTCCG TGGATTGAAG 
AGGTTGGACA ACTATTGTAA GCAATTCATC ATCGAAGATC AACAAGTAAG ATTAGATCCA 
TACGTTACCG CCGAAGATAT AGAGATCATG GACATGGAAC GTGAACGTAG GCTTGATGAA 
TTTGAGGAGT TCCACGTGCC AGAAAGGATT ATTGATAGCC AACGTGCTTC GTTGGAAGAC 
GGCACCTCAC AATTACAGTA TTTAGTTAAA TGGCGTCGTC TAAATTACGA CGAGGCTACT 
TGGGAGAATG CCACAGATAT CGTGAAATTG GCACCTGAAC AAGTGAAACA TTTTCAAAAC 
AGAGAAAACT CTAAGATCCT CCCACAATAT TCCAGTAATT ATACTTCACA GAGACCGCGT 
TTTGAGAAGT TAAGCGTGCA ACCTCCGTTC ATTAAAGGTG GGGAATTAAG AGATTTTCAA 
CTAACTGGTA TTAATTGGAT GGCATTTTTG TGGTCCAAAG GTGATAATGG TATACTGGCA 
GATGAGATGG GCCTGGGAAA AACGGTCCAG ACTGTCGCCT TTATCAGTTG GCTGATATTT 
GCTCGTAGAC AAAACGGACC TCACATCATT GTCGTTCCTT TATCGACAAT GCCTGCCTGG 
TTGGATACTT TTGAGAAATG GGCGCCTGAT TTGAATTGTA TATGCTATAT GGGCAACCAA 
AAATCAAGAG ATACCATTCG AGAATATGAA TTTTACACCA ATCCAAGGGC AAAAGGGAAA 
AAAACAATGA AATTTAATGT TTTATTAACA ACATACGAGT ACATCTTAAA GGATCGTGCT 
GAATTAGGAA GTATAAAATG GCAATTTATG GCCGTTGACG AAGCTCATAG ACTAAAAAAT 
GCTGAATCAT CCCTTTATGA ATCATTAAAC AGTTTCAAGG TCGCCAACCG TATGTTAATC 
ACAGGCACAC CTCTTCAGAA TAATATTAAA GAGTTAGCTG CGTTGGTTAA TTTCCTAATG 
CCCGGAAGGT TTACGATTGA TCAGGAGATT GATTTTGAAA ACCAAGATGA AGAGCAAGAA 
GAATATATTC ATGATTTACA CCGAAGAATA CAGCCTTTTA TTCTTCGTCG GTTGAAGAAA 
GACGTAGAAA AATCACTTCC ATCAAAGACA GAGCGTATTT TAAGAGTTGA ATTGTCCGAC 
GTACAGACTG AGTACTATAA AAATATTCTG ACTAAAAACT ACTCTGCTTT AACTGCTGGA 
GCTAAAGGGG GTCATTTCTC TTTACTGAAT ATTATGAACG AGTTGAAAAA GGCATCGAAC 
CATCCATATC TCTTCGATAA TGCTGAAGAG CGCGTCTTAC AGAAATTTGG GGATGGTAAA 
ATGACTCGAG AAAACGTACT AAGAGGTTTG ATCATGTCTT CGGGTAAGAT GGTTCTTTTA 
GACCAATTAT TGACCAGATT GAAGAAAGAT GGGCACCGCG TGTTGATTTT TTCACAAATG 
GTCAGAATGC TTGACATTTT AGGCGACTAT TTATCCATTA AAGGTATTAA CTTCCAAAGG 
TTAGATGGTA CAGTACCATC TGCTCAAAGA AGGATATCTA TTGATCATTT CAACTCTCCG 
GATTCAAACG ACTTCGTATT TTTACTTTCT ACTCGTGCTG GTGGTTTGGG TATCAACTTA 
ATGACCGCCG ATACCGTTGT GATTTTTGAT TCCGATTGGA ATCCGCAAGC CGATTTACAA 
GCAATGGCTA GAGCTCATCG TATTGGCCAA AAAAATCACG TTATGGTGTA TAGGTTGGTT 
TCAAAAGACA CAGTAGAGGA AGAGGTATTA GAAAGAGCAC GGAAGAAAAT GATTCTGGAA 
TATGCTATTA TTTCTCTTGG AGTGACAGAT GGTAACAAAT ACACTAAGAA GAATGAACCA 
AATGCCGGTG AATTATCGGC AATCCTGAAG TTCGGTGCAG GCAATATGTT CACTGCCACA 
GACAACCAGA AAAAATTGGA GGATCTTAAC TTGGATGATG TTTTGAATCA TGCAGAGGAT 
CACGTTACTA CTCCAGACTT AGGAGAGTCG CATCTTGGCG GTGAAGAGTT TTTGAAGCAA 
TTCGAAGTGA CTGATTATAA AGCTGATATA GATTGGGATG ATATCATTCC AGAAGAAGAA 
CTAAAAAAAC TCCAAGATGA AGAGCAGAAA CGCAAGGATG AAGAATATGT TAAAGAACAA 
CTTGAAATGA TGAATAGAAG GGATAACGCC CTAAAAAAAA TAAAAAACAG TGTTAATGGT 
GATGGGACCG CCGCAAACTC AGATTCTGAC GACGATAGTA CTTCCAGATC TTCTAGAAGA 
AGAGCAAGAG CTAATGACAT GGACTCTATT GGTGAATCGG AGGTGAGAGC TTTGTATAAA 
GCTATTTTAA AATTTGGCAA CCTAAAGGAG ATTTTGGACG AGTTGATTGC AGATGGAACC 
CTGCCGGTCA AATCATTTGA AAAATACGGT GAAACTTACG ATGAAATGAT GGAAGCAGCT 
AAAGACTGTG TACACGAGGA AGAGAAAAAT AGAAAAGAAA TCTTGGAGAA ACTTGAGAAG 
CATGCTACCG CCTATAGAGC AAAGCTAAAA AGCGGTGAAA TAAAAGCAGA GAACCAACCA 
AAGGATAATC CGTTGACTAG ATTATCTTTA AAAAAAAGAG AAAAGAAAGC CGTCCTTTTC 
AACTTTAAAG GTGTTAAATC TTTAAACGCC GAATCTTTAC TAAGTAGAGT GGAGGATTTA 
AAGTACTTGA AGAACTTGAT AAATTCAAAC TACAAAGATG ATCCATTAAA GTTTAGCCTA 
GGCAACAACA CACCTAAGCC TGTACAGAAT TGGTCATCTA ATTGGACGAA AGAGGAAGAT 
GAGAAGCTAT TGATTGGTGT TTTCAAATAT GGATATGGTT CCTGGACACA AATAAGAGAC 
GATCCATTTC TAGGCATTAC TGATAAAATA TTCTTGAATG AAGTTCACAA TCCGGTAGCA 
AAAAAGTCGG CAAGCTCCTC TGATACAACA CCAACACCCT CAAAGAAAGG AAAGGGAATA 
ACGGGTTCTT CAAAAAAAGT ACCTGGTGCC ATTCACTTGG GCAGAAGAGT TGATTATCTA 
TTATCCTTCT TAAGAGGAGG CCTAAATACA AAGAGTCCCA GTGCTGACAT AGGCTCGAAA 
AAACTCCCTA CTGGTCCTTC CAAAAAGAGA CAAAGAAAAC CTGCTAATCA CAGCAAATCC 
ATGACTCCAG AAATTACAAG TTCTGAGCCT GCAAATGGCC CGCCAAGCAA ACGTATGAAA 
GCCCTCCCAA AAGGTCCTGC CGCCTTAATA AATAACACGA GATTGTCTCC AAACAGCCCC 
ACGCCCCCAT TAAAGTCCAA GGTTTCCAGA GATAATGGTA CAAGGCAATC AAGCAACCCC 
AGTAGCGGCA GCGCTCACGA AAAAGAATAT GACAGCATGG ATGAAGAAGA CTGTCGACAC 
ACAATGAGTG CAATACGTAC TTCTTTGAAG CGACTTAGAA GAGGCGGCAA AAGTCTTGAT 
CGTAAGGAAT GGGCCAAAAT TTTGAAGACC GAATTAACTA CGATTGGTAA TCATATCGAA 
TCGCAAAAGG GCTCATCGAG AAAGGCAAGC CCAGAAAAAT ATCGCAAGCA CCTTTGGTCT 
TACAGTGCCA ACTTTTGGCC TGCCGACGTT AAGAGTACAA AGCTGATGGC AATGTACGAC 
AAGATAACAG AGTCTCAAAA GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011091.1
CDS	1..4407
Translation	MAAKDISTEVLQNPELYGLRRSHRAAAHQQNYFNDSDDEDDEDNIKQSRRKRMTTIEDDEDEFEDEEGEEDSGEDEDEEDFEEDDDYYGSPIKQNRSKPKSRTKSKSKSKPKSQSEKQSTVKIPTRFSNRQNKTVNYNIDYSDDDLLESEDDYGSEEALSEENVHEASANPQPEDFHGIDIVINHRLKTSLEEGKVLEKTVPDLNNCKENYEFLIKWTDESHLHNTWETYESIGQVRGLKRLDNYCKQFIIEDQQVRLDPYVTAEDIEIMDMERERRLDEFEEFHVPERIIDSQRASLEDGTSQLQYLVKWRRLNYDEATWENATDIVKLAPEQVKHFQNRENSKILPQYSSNYTSQRPRFEKLSVQPPFIKGGELRDFQLTGINWMAFLWSKGDNGILADEMGLGKTVQTVAFISWLIFARRQNGPHIIVVPLSTMPAWLDTFEKWAPDLNCICYMGNQKSRDTIREYEFYTNPRAKGKKTMKFNVLLTTYEYILKDRAELGSIKWQFMAVDEAHRLKNAESSLYESLNSFKVANRMLITGTPLQNNIKELAALVNFLMPGRFTIDQEIDFENQDEEQEEYIHDLHRRIQPFILRRLKKDVEKSLPSKTERILRVELSDVQTEYYKNILTKNYSALTAGAKGGHFSLLNIMNELKKASNHPYLFDNAEERVLQKFGDGKMTRENVLRGLIMSSGKMVLLDQLLTRLKKDGHRVLIFSQMVRMLDILGDYLSIKGINFQRLDGTVPSAQRRISIDHFNSPDSNDFVFLLSTRAGGLGINLMTADTVVIFDSDWNPQADLQAMARAHRIGQKNHVMVYRLVSKDTVEEEVLERARKKMILEYAIISLGVTDGNKYTKKNEPNAGELSAILKFGAGNMFTATDNQKKLEDLNLDDVLNHAEDHVTTPDLGESHLGGEEFLKQFEVTDYKADIDWDDIIPEEELKKLQDEEQKRKDEEYVKEQLEMMNRRDNALKKIKNSVNGDGTAANSDSDDDSTSRSSRRRARANDMDSIGESEVRALYKAILKFGNLKEILDELIADGTLPVKSFEKYGETYDEMMEAAKDCVHEEEKNRKEILEKLEKHATAYRAKLKSGEIKAENQPKDNPLTRLSLKKREKKAVLFNFKGVKSLNAESLLSRVEDLKYLKNLINSNYKDDPLKFSLGNNTPKPVQNWSSNWTKEEDEKLLIGVFKYGYGSWTQIRDDPFLGITDKIFLNEVHNPVAKKSASSSDTTPTPSKKGKGITGSSKKVPGAIHLGRRVDYLLSFLRGGLNTKSPSADIGSKKLPTGPSKKRQRKPANHSKSMTPEITSSEPANGPPSKRMKALPKGPAALINNTRLSPNSPTPPLKSKVSRDNGTRQSSNPSSGSAHEKEYDSMDEEDCRHTMSAIRTSLKRLRRGGKSLDRKEWAKILKTELTTIGNHIESQKGSSRKASPEKYRKHLWSYSANFWPADVKSTKLMAMYDKITESQKK

Target ORF information:

RefSeq Version	NM_001179054.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C chromatin-remodeling ATPase CHD1 (CHD1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179054.1

ORF Insert Sequence:

ATGGCAGCCA AGGATATTTC TACTGAAGTG CTGCAAAATC CTGAACTATA TGGGCTGAGG 
CGGTCACATA GAGCTGCTGC GCATCAACAA AACTACTTCA ACGATAGCGA TGATGAGGAC 
GATGAAGATA ATATTAAACA GTCGAGGAGG AAGAGAATGA CCACGATTGA AGACGACGAA 
GACGAATTTG AAGATGAAGA AGGCGAAGAA GATTCTGGAG AGGACGAAGA TGAGGAAGAT 
TTTGAAGAAG ATGATGATTA TTATGGCTCT CCTATAAAAC AAAATCGATC AAAACCGAAA 
TCAAGAACAA AATCAAAGTC TAAATCTAAG CCAAAATCTC AATCTGAAAA GCAATCTACA 
GTGAAAATAC CAACAAGGTT TTCTAATCGT CAAAACAAAA CGGTAAATTA TAACATCGAT 
TATAGCGATG ATGACCTCTT AGAATCTGAA GATGACTATG GTTCCGAGGA GGCTTTATCA 
GAGGAAAATG TGCATGAAGC ATCTGCCAAT CCTCAACCAG AGGACTTCCA CGGTATTGAT 
ATTGTTATCA ATCACAGACT AAAGACATCT TTGGAAGAAG GAAAGGTCTT GGAAAAGACT 
GTTCCTGACC TCAATAACTG CAAGGAAAAT TATGAATTCT TAATCAAATG GACAGATGAA 
TCTCATCTCC ACAATACCTG GGAAACTTAT GAATCCATTG GCCAAGTCCG TGGATTGAAG 
AGGTTGGACA ACTATTGTAA GCAATTCATC ATCGAAGATC AACAAGTAAG ATTAGATCCA 
TACGTTACCG CCGAAGATAT AGAGATCATG GACATGGAAC GTGAACGTAG GCTTGATGAA 
TTTGAGGAGT TCCACGTGCC AGAAAGGATT ATTGATAGCC AACGTGCTTC GTTGGAAGAC 
GGCACCTCAC AATTACAGTA TTTAGTTAAA TGGCGTCGTC TAAATTACGA CGAGGCTACT 
TGGGAGAATG CCACAGATAT CGTGAAATTG GCACCTGAAC AAGTGAAACA TTTTCAAAAC 
AGAGAAAACT CTAAGATCCT CCCACAATAT TCCAGTAATT ATACTTCACA GAGACCGCGT 
TTTGAGAAGT TAAGCGTGCA ACCTCCGTTC ATTAAAGGTG GGGAATTAAG AGATTTTCAA 
CTAACTGGTA TTAATTGGAT GGCATTTTTG TGGTCCAAAG GTGATAATGG TATACTGGCA 
GATGAGATGG GCCTGGGAAA AACGGTCCAG ACTGTCGCCT TTATCAGTTG GCTGATATTT 
GCTCGTAGAC AAAACGGACC TCACATCATT GTCGTTCCTT TATCGACAAT GCCTGCCTGG 
TTGGATACTT TTGAGAAATG GGCGCCTGAT TTGAATTGTA TATGCTATAT GGGCAACCAA 
AAATCAAGAG ATACCATTCG AGAATATGAA TTTTACACCA ATCCAAGGGC AAAAGGGAAA 
AAAACAATGA AATTTAATGT TTTATTAACA ACATACGAGT ACATCTTAAA GGATCGTGCT 
GAATTAGGAA GTATAAAATG GCAATTTATG GCCGTTGACG AAGCTCATAG ACTAAAAAAT 
GCTGAATCAT CCCTTTATGA ATCATTAAAC AGTTTCAAGG TCGCCAACCG TATGTTAATC 
ACAGGCACAC CTCTTCAGAA TAATATTAAA GAGTTAGCTG CGTTGGTTAA TTTCCTAATG 
CCCGGAAGGT TTACGATTGA TCAGGAGATT GATTTTGAAA ACCAAGATGA AGAGCAAGAA 
GAATATATTC ATGATTTACA CCGAAGAATA CAGCCTTTTA TTCTTCGTCG GTTGAAGAAA 
GACGTAGAAA AATCACTTCC ATCAAAGACA GAGCGTATTT TAAGAGTTGA ATTGTCCGAC 
GTACAGACTG AGTACTATAA AAATATTCTG ACTAAAAACT ACTCTGCTTT AACTGCTGGA 
GCTAAAGGGG GTCATTTCTC TTTACTGAAT ATTATGAACG AGTTGAAAAA GGCATCGAAC 
CATCCATATC TCTTCGATAA TGCTGAAGAG CGCGTCTTAC AGAAATTTGG GGATGGTAAA 
ATGACTCGAG AAAACGTACT AAGAGGTTTG ATCATGTCTT CGGGTAAGAT GGTTCTTTTA 
GACCAATTAT TGACCAGATT GAAGAAAGAT GGGCACCGCG TGTTGATTTT TTCACAAATG 
GTCAGAATGC TTGACATTTT AGGCGACTAT TTATCCATTA AAGGTATTAA CTTCCAAAGG 
TTAGATGGTA CAGTACCATC TGCTCAAAGA AGGATATCTA TTGATCATTT CAACTCTCCG 
GATTCAAACG ACTTCGTATT TTTACTTTCT ACTCGTGCTG GTGGTTTGGG TATCAACTTA 
ATGACCGCCG ATACCGTTGT GATTTTTGAT TCCGATTGGA ATCCGCAAGC CGATTTACAA 
GCAATGGCTA GAGCTCATCG TATTGGCCAA AAAAATCACG TTATGGTGTA TAGGTTGGTT 
TCAAAAGACA CAGTAGAGGA AGAGGTATTA GAAAGAGCAC GGAAGAAAAT GATTCTGGAA 
TATGCTATTA TTTCTCTTGG AGTGACAGAT GGTAACAAAT ACACTAAGAA GAATGAACCA 
AATGCCGGTG AATTATCGGC AATCCTGAAG TTCGGTGCAG GCAATATGTT CACTGCCACA 
GACAACCAGA AAAAATTGGA GGATCTTAAC TTGGATGATG TTTTGAATCA TGCAGAGGAT 
CACGTTACTA CTCCAGACTT AGGAGAGTCG CATCTTGGCG GTGAAGAGTT TTTGAAGCAA 
TTCGAAGTGA CTGATTATAA AGCTGATATA GATTGGGATG ATATCATTCC AGAAGAAGAA 
CTAAAAAAAC TCCAAGATGA AGAGCAGAAA CGCAAGGATG AAGAATATGT TAAAGAACAA 
CTTGAAATGA TGAATAGAAG GGATAACGCC CTAAAAAAAA TAAAAAACAG TGTTAATGGT 
GATGGGACCG CCGCAAACTC AGATTCTGAC GACGATAGTA CTTCCAGATC TTCTAGAAGA 
AGAGCAAGAG CTAATGACAT GGACTCTATT GGTGAATCGG AGGTGAGAGC TTTGTATAAA 
GCTATTTTAA AATTTGGCAA CCTAAAGGAG ATTTTGGACG AGTTGATTGC AGATGGAACC 
CTGCCGGTCA AATCATTTGA AAAATACGGT GAAACTTACG ATGAAATGAT GGAAGCAGCT 
AAAGACTGTG TACACGAGGA AGAGAAAAAT AGAAAAGAAA TCTTGGAGAA ACTTGAGAAG 
CATGCTACCG CCTATAGAGC AAAGCTAAAA AGCGGTGAAA TAAAAGCAGA GAACCAACCA 
AAGGATAATC CGTTGACTAG ATTATCTTTA AAAAAAAGAG AAAAGAAAGC CGTCCTTTTC 
AACTTTAAAG GTGTTAAATC TTTAAACGCC GAATCTTTAC TAAGTAGAGT GGAGGATTTA 
AAGTACTTGA AGAACTTGAT AAATTCAAAC TACAAAGATG ATCCATTAAA GTTTAGCCTA 
GGCAACAACA CACCTAAGCC TGTACAGAAT TGGTCATCTA ATTGGACGAA AGAGGAAGAT 
GAGAAGCTAT TGATTGGTGT TTTCAAATAT GGATATGGTT CCTGGACACA AATAAGAGAC 
GATCCATTTC TAGGCATTAC TGATAAAATA TTCTTGAATG AAGTTCACAA TCCGGTAGCA 
AAAAAGTCGG CAAGCTCCTC TGATACAACA CCAACACCCT CAAAGAAAGG AAAGGGAATA 
ACGGGTTCTT CAAAAAAAGT ACCTGGTGCC ATTCACTTGG GCAGAAGAGT TGATTATCTA 
TTATCCTTCT TAAGAGGAGG CCTAAATACA AAGAGTCCCA GTGCTGACAT AGGCTCGAAA 
AAACTCCCTA CTGGTCCTTC CAAAAAGAGA CAAAGAAAAC CTGCTAATCA CAGCAAATCC 
ATGACTCCAG AAATTACAAG TTCTGAGCCT GCAAATGGCC CGCCAAGCAA ACGTATGAAA 
GCCCTCCCAA AAGGTCCTGC CGCCTTAATA AATAACACGA GATTGTCTCC AAACAGCCCC 
ACGCCCCCAT TAAAGTCCAA GGTTTCCAGA GATAATGGTA CAAGGCAATC AAGCAACCCC 
AGTAGCGGCA GCGCTCACGA AAAAGAATAT GACAGCATGG ATGAAGAAGA CTGTCGACAC 
ACAATGAGTG CAATACGTAC TTCTTTGAAG CGACTTAGAA GAGGCGGCAA AAGTCTTGAT 
CGTAAGGAAT GGGCCAAAAT TTTGAAGACC GAATTAACTA CGATTGGTAA TCATATCGAA 
TCGCAAAAGG GCTCATCGAG AAAGGCAAGC CCAGAAAAAT ATCGCAAGCA CCTTTGGTCT 
TACAGTGCCA ACTTTTGGCC TGCCGACGTT AAGAGTACAA AGCTGATGGC AATGTACGAC 
AAGATAACAG AGTCTCAAAA GAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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CHD1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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