BEM2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	BEM2
Entrez Gene ID	856899
Full Name	Bem2p
Synonyms	IPL2,SUP9,TSL1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179045.3	NP_011082.3	Bem2p

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AGR144C	NP_986810.1
Saccharomyces cerevisiae (baker's yeast)	BEM2	NP_011082.3
Kluyveromyces lactis	KLLA0E00903g	XP_453989.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Dietrich FS, Mulligan J, Hennessy K, Yelton MA, Allen E, Araujo R, Aviles E, Berno A, Brennan T, Carpenter J, Chen E, Cherry JM, Chung E, Duncan M, Guzman E, Hartzell G, Hunicke-Smith S, Hyman RW, Kayser A, Komp C, Lashkari D, Lew H, Lin D, Mosedale D, Davis RW
Nature387(6632 Suppl)78-81(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bem2 might be a novel GTPase-activating protein for Rho4 in Saccharomyces cerevisiae.
Title: Control of polarized growth by the Rho family GTPase Rho4 in budding yeast: requirement of the N-terminal extension of Rho4 and regulation by the Rho GTPase-activating protein Bem2.

Bem2p and Bem3p may function as global inhibitors of Cdc42p activation during G1, and their inactivation by the Cdc28p/Cln kinase contributes to site-specific activation of Cdc42p at bud emergence
Title: Phosphorylation of Bem2p and Bem3p may contribute to local activation of Cdc42p at bud emergence.

The following BEM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BEM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05650	NM_001179045.3 Latest version!	Saccharomyces cerevisiae S288C Bem2p (BEM2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BEM2 ( NM_001179045.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011082.3 Saccharomyces cerevisiae S288C Bem2p (BEM2), partial mRNA.

Price & Availability↑

CloneID	OSi05650
Clone ID Related Accession (Same CDS sequence)	NM_001179045.3
Accession Version	NM_001179045.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Bem2p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001137). On Jul 30, 2012 this sequence version replaced NM_001179045.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGAAAGGTC TTCTCTGGTC TAAGAACAGG AAATCTTCAA CGGCCAGTGC TAGTTCAAGC 
TCAACTTCCA CGTCTCATAA GACAACTACT GCGTCTACAG CATCGTCGTC GTCGCCGTCA 
TCATCCTCTC AAACTATTCG AAACTCGACA AGCGGTGCTT CTCCATACAT GCATTCCCAC 
CATCACCATG GCCAGGGACA CAGTCACCAC AGAGGGGAAG ACAACAACAG AGATAAGAGA 
AAAAGCTCCG TATTTCCACC CTCCAAGCAG TACACATCCA CTAGTTCCAG TCAGGTGAAC 
TTGGGGATGT ATCATTCGGA TACTAACACC AGATCTTCTA GAAGCATAGC CTCTACGCTA 
AAAGACGATT CTCCGTCTGT CTGCTCCGAA GATGAAATAA GCAATTCTTC TAGCCAGAAG 
TCCAATGCCC AGGATGAGAC TCCCATTGCG TACAAGAAGA GTGCCCATTC GAAAGACTCA 
TTGTTGCCTT CACGTTCCTC GTCTTTATCA CCTCCTCAGT CTCGATGTAG CACTGGAACT 
ACGCTAGAGA AATCGTTGAA CACTTCAGGT ATCAGTAACA GTAGCGGCAC TAATAATAAC 
AATAGCAATA ATAACAACGA TAACGAGCAA AAGCAAAGAA ATGTTATCCA CTTAAACTCT 
GAAAACTATG ATACAACTGT TTTTAAGACT GGATGGGTTA ATAAGTCCCA TGGCCAGACT 
GTTGCTACAA ACTATAATAG CTCAATGACA GCCCCTTCCT CTTCCTCTTC GTCCTCCTCT 
CAGAATCTCC GTAATGATGC GTATTCTAGA AACAGGGAAA GCAGGTTTTA TGGGAACGAC 
GGTTCGTCTT TAAAAAATGA TGACAGCAGT AGTACTACGG CCACTAACAG TGGCAATGAT 
GTGGCCTCAG CAAGAAGCAG CATGGCTATA GATCCTCAAA TGCTAGTACC TGATTACCGT 
CTTTATAGGG CTCAATTGAA AGGTTGCGTT CTCAACCTTT ACAAAAGTGG TTTGAATAGT 
AACATTAAGT TTTTTGATCC AACTTTACCA GCTTCAAATA GTAGTATAGC AAATGAAAAC 
CATCAACAGA AAAAACAACA AACAAACAAC CAAGCACAAG CGGAAGCTCT GCACCAAAAG 
CAATCATTTG GCCAAATGGG TGAACCTATT ACACTGGACC TGAAGTATTT ATCTGAAGTT 
TACCCGCACC CAGATTTAAG ACAAGACAGT GATGGTAAAA TTATATCAGG CACAATAGAA 
AGTTTATGTC ACACAGTCTT ATTTTACCCC GGTCCAAAGC AATCGGACGT ACCTAATGAG 
AAATCATTAT CTAAGACACA TAGAGCTGTT ATTAATCTGT TATTGATGTT TCCATTACTA 
GATCATTTCA TTAAGTTTCT AAAGGTATTC AACCAATTCG GTCTTTCTTT TACCAAGAAT 
AAATCAAGAT TGACAAATAA TTCCACACAA TTCTACAACA TATCCCCTGC TGTCGATGAC 
TCCATGACTC AAAGATTAGC TTTAACTGCT AAAACAATCC TTGATGTATT TCCGGGATTT 
TTGCTGGATG AACCAATGCT GAAGACTATA ATTTCCTTAT TAGATACTAT ATCTTTGCAT 
AACGATGAAA TTTCCAACAA TTTGAAAATT AAAATCGCCA ATAAACATAA CGAATTAATG 
AAACTTACCG CATTCACTCG ATCTCTTCCA ATGGCAACAT CATCTACACA TGAATTAGAG 
ATTATATTGG ATCCAAGTCA TTTCCTGTCT TTAGACATTA CAACATTGGC TGATGAAGTT 
CATCATATCA ATTTAAAGTT TGATAAAGTA TGGGCACCGA AATTTGACTA TTCTCTGCTG 
TATGATTCGA AGTTTATTAA TCGAAGAATT GTCTCGTTAA ATCCATTGGT ATTCAACAAC 
GACCAAAATA TCCATTTTCT TGGAAGGCTA CTAATATCAC ATTTATTTCC AACAAATCCT 
GAGTTTAGTA AAAAAGTGAC TCCAAAAGTG AGAGCTGAGT TATTAGATAA ATGGGTCCAA 
ATTGGCTGTA GATTTGAACA TTTAGGAGAC ATGGTATCAT GGCTAGCTGT CGCCACTATC 
ATTTGTTCAA TTCCTGTACT AAGGTCATCT TCATGGAAAT ATGTTCCTGA TCAGTCATTG 
AAAACTATTT TTAAAGATTG GGTTCCTACC ATTATTCAGT TAGAGAGGCG ACAAAGGACA 
TCTAAATCTA CAAGTAGTGT TTTCATACTC GCTCCTCCAA ATCTGGATGA CGACTTTACG 
AGAGCTAACG TGATTTCTTA TTTTGGCGAT TTATTAATTC ATGCTGATGA TTTACCATCT 
GATACGAAAT TCAAGTACTT GGAAAAGAAA ATTAACAGAA CAAAAAACGC TTTTCATAAA 
TGGCAACAAA GATTACAAGC AATCGACTCT ACGAGACATA AAACTAATTC GACAGAAAAC 
GTCAGGGATA ATGATTCTCC TAATAACGTT GTTTATCAGC TCTGGAAATT TCATTTATCA 
CAACCTCCGT TGAACATTGA AGGAATAATG AAATTAAGTG TCCAACACGA ACCTCCAATA 
ATCGACCAGA AAGCGTATTC TACTATTGGT TCTCAAAGGA GTGCTTTAGT AACTGGAAGT 
TATTTACCCA TTTTATTCAA TGAACTATTC CCAAATTATT CACTATTTCC TAAGAATACA 
TTAGTGGGTG CTGCCAGTGA TGCGAAGTTG CCGCCTCCAA GATCATCCGC GCGCCTCTCA 
AAATCACTTT CAATCAGTGA ACCAATTCCA ATAGCTTCAA ACAGTCACAC TATGGGCTCA 
TTAACAGATG ATGCTATGTC CTCAAAAAAT GATAATAATA AAGTTACAGG TGTTGGAAAA 
ATTGACGGAC CTGTGATCAA AGAGATGTCG TCAAAACAAT CAAATAAACA GAGACTATTA 
AAATCTGTGA GAGATGTTTT TAATATTGAC ATGGATGTGT TTCATATATC AGATGAGTTA 
GTATTTAAAT CGGTATACGA TAATGATGGT AAATCCCGTC CTGCTAGTAT GGTCATAGAA 
ACACCAAAGA GATTCTCACA ACACTCTTCC ATGCTTATTA ATAATCCAGC TACTCCAAAC 
CAAAAGATGA GAGATTCCTT GGATACCACT GGAAGACTAA GTAAGACATT AGAAAATATG 
GACTTTTTCA ACAATATTGG TCAAGTATCA GATTCACTAA AAGAATCGAT TATTCGTGTC 
GTATTGAAAT CGTCGTCGCT AGAAAAGATT TTTGATTTAC TGGTATTAAC TTCAAATATC 
TTTTCAAAAT TGGTAGACAC TAAAGACCTT GAGAATTATT ACTATCATCA AAGACAAAGG 
GGTCATTCTA CCAGAGGGCT TTCCGATGAT AACATAGGAC TACTAGATTA TGCATTCGTT 
AAACTAACTA TGGACAATGA TATTTTCACA GAAACATTCT TCAACACTTA TAAAAGCTTT 
ACTACTACAA CTACGGTATT AGAAAATATG GCAAAACGGT ATGTTGGTGC AAAGAGTTGC 
AGCGTGTCAA TTTCCAAGAT TTTAGATCGC AGTGATGATT CTAAAATGAA GATAAATGAG 
GATACAAATT TAGTGTCCTC TTCATTATAC GACCAAAATT TTCCTGTTTG GGATATGAAA 
GTAACTGACG ATGAAAACAT AAACCTTATT TATATGGCAA AAATCCAAAT CGGCGCCGCG 
GAAGCTATAC TACATTTAGT GAAAAATCAT TACTCTGACT TTACTGACGA TTTATGTAAC 
AACTCTACTC TATTGGATAT TATAAAAATA ATGGAACAAG AAGTATCCAC TGAATGGCCT 
ACGCGAATTG CAAACTCAAA GTTACAAAAA AGCCTACCAG AAAATTTTGT GATTGAAACA 
GAAAATTTGT TGACTACGTT AACTGATCTA TTTCATGGTA TTAAATCAGC ATACCAAAAG 
CAACTATACA GACCTATTGG TGTCAATAGA ACTCAGAAGA GAATAACTGA CATTTTGAAT 
TCCTTTAACA CATTCTCTTT TACAGATCTT AACAACATCA TTGATGATCC TTCCTTTAGC 
GATGACATGA TTAGATCTTT TCAAAAGTTA CACTCAACCA ATTACGAAGA TATACTAGAA 
TGGATCTACC AGTTAGATAA CTTTATTTCT AAGAAGTTCA ATTTAGTTTC TAAGAAAGAT 
TGGATAGTTC TTTTCCAGGA GTTGGAATTG CTTTCAAAGG AATCTCTGGT CTCTTTTTTC 
AATTATCCGC TACATTTTAA GTCAAGCAAA TTGATAAACC CCGGATATTT GCAATTGCAC 
GAGTTTGAAA TTTCCAATCT TTTTACTTGG ATTTCTACAT TGATTCTTAA GGATGATAAT 
GGTACGGAGA GTCTTTTTTT TGAAAAACTA CCACAATCTA TAAAATTGTT AATAAAGTTA 
CACACGAGTC TAACTACGTT TTTCGTGATG GAAATTTCAA ACGTCAACAA AAGCAGTAGT 
GAAAGGTTAA CTACTTGTAA AGTTATATTA CAAATTTTGA ACTACATCCG CTGGAAAAAC 
GGAAGTTTGG ATTTATTTGA TTCAGAGGAG GATGAAAGTC CTCATGCTAT TTGTCCACAC 
ATTCCAGCTT TTATTGAAAC TGCTATTGCG CATGCCATAA TATCACCAGA GTCTAGAAAC 
TATGAACTTT CGTGGATCAA AGCTTCTGAA AAACTTTCGG ACCCTACTAA AGGAACCCAA 
AATCTAAGAT CGATTAGTAA CGTTTTAGAG AAGATAGATG ACATACATAT AAAGAGATTC 
ATTGAGATTG ATGATGTATT CAGCAAAAAC TGCAAGAATT TATGCCCCTG CCCTGGTTGG 
TTCATTTCAA GGTTACTAGA AATATCTCAG TTTGTCCCTA ACATGAGTAT CACTAATTCG 
AAACTTATTA ATTTTGACAA AAGGAGATTT GTTAACAACA TAATATCTAA TGTCTTGGAT 
TTAATTCCGA ACGAAAGGGA GTTTCCACTT GATATTGAAA TGAGTGACGA AAATCCTTCG 
AAAAGAACTA CATTTGGCCG CATTCTGTTC AATAATTTTG AAGATGTTAA TAAAGTATAC 
AGAAAGAAGA CTAAAAAAGT ATCAGAATCA GAAGCCATAT CGGAGAGATT TCAAGAGCAG 
GGAGTATTTA ATGAAATACT AGTCAATGAA ATTGAAAAAA TAAAGAGGGA AGCCAGAAAA 
TTAGAGGTTT TACTCGATCA AGAAAAGATC TTGAAGAACT CTGCTGCTTT ACATCAGGCC 
GTTCCGAAAA AGAATAGGAA GTCTGTTATT ATCTCAGGTA CTCATAGTGA CAACGATCAT 
AGCTATAATA TCAATAAGAA CACCGGTCAA ACGCCTTCTC TAGGTAGTGT TATGGAAAGC 
AATAATTCAG CAAGGAATAG AAGAGATTCA AGGGCCTCCT TTTCCACAAA TAGATCATCA 
GTAGTGTCAA ATTCCAGCCA TAATGGTGTT AGTAAAAAAA TAGGTGGATT TTTCAGAAGA 
CCATTTTCAA TCGGTGGATT CAACACATCC AGTTCTAATT ATTCACTCAA CAGTATATTA 
TCGCAAGAAG TTTCAAGTAA CAAATCCATT CTACCAAGTA TACTTCCAGA GGTTGATTCC 
ATGCAACTAC ACGACTTAAA ACCTAGTTAT AGTTTGAAGA CCTTTGAAAT CAAGTCAATA 
ATGGAAATAA TCAATCACAG AAACATTCCG GCTTACTACT ATGCGTTCAA GATAGTTATG 
CAAAATGGCC ATGAATATCT GATTCAAACG GCTAGTTCTA GTGACCTCAC AGAATGGATA 
AAAATGATCA AGGCTTCCAA GAGATTTTCA TTCCATTCGA AAAAATATAA AGGTAAAACT 
CATAATAAGA TATTTGGGGT ACCATTAGAA GACGTTTGTG AAAGAGAGAA TACCTTGATA 
CCTACAATTG TTGTGAAATT GCTGGAAGAG ATTGAGCTGC GTGGGCTAGA TGAAGTGGGA 
TTGTACAGAA TTCCTGGTTC CATCGGCAGT ATAAATGCAC TAAAGAACGC ATTTGACGAA 
GAAGGTGCAA CAGATAATTC CTTTACATTA GAGGACGATA GATGGTTTGA AGTGAATGCG 
ATCGCAGGCT GTTTTAAGAT GTATTTAAGA GAGTTACCAG ATTCTTTATT TTCTCATGCT 
ATGGTGAACG ACTTTACCGA TTTGGCGATT AAGTATAAAG CACATGCAAT GGTAAATGAA 
GAATACAAGA GAATGATGAA TGAGTTACTC CAAAAGTTAC CTACATGCTA TTACCAAACT 
TTGAAAAGAA TTGTATTTCA TTTGAATAAA GTACATCAGC ATGTTGTAAA TAATAAAATG 
GACGCATCAA ACTTGGCTAT TGTATTTTCC ATGAGTTTTA TAAACCAAGA AGACTTAGCC 
AACAGCATGG GGTCCAGATT AGGTGCAGTT CAGACAATCC TGCAAGATTT TATTAAGAAT 
CCAAATGATT ATTTCAAGCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011082.3
CDS	1..6504
Translation	MKGLLWSKNRKSSTASASSSSTSTSHKTTTASTASSSSPSSSSQTIRNSTSGASPYMHSHHHHGQGHSHHRGEDNNRDKRKSSVFPPSKQYTSTSSSQVNLGMYHSDTNTRSSRSIASTLKDDSPSVCSEDEISNSSSQKSNAQDETPIAYKKSAHSKDSLLPSRSSSLSPPQSRCSTGTTLEKSLNTSGISNSSGTNNNNSNNNNDNEQKQRNVIHLNSENYDTTVFKTGWVNKSHGQTVATNYNSSMTAPSSSSSSSSQNLRNDAYSRNRESRFYGNDGSSLKNDDSSSTTATNSGNDVASARSSMAIDPQMLVPDYRLYRAQLKGCVLNLYKSGLNSNIKFFDPTLPASNSSIANENHQQKKQQTNNQAQAEALHQKQSFGQMGEPITLDLKYLSEVYPHPDLRQDSDGKIISGTIESLCHTVLFYPGPKQSDVPNEKSLSKTHRAVINLLLMFPLLDHFIKFLKVFNQFGLSFTKNKSRLTNNSTQFYNISPAVDDSMTQRLALTAKTILDVFPGFLLDEPMLKTIISLLDTISLHNDEISNNLKIKIANKHNELMKLTAFTRSLPMATSSTHELEIILDPSHFLSLDITTLADEVHHINLKFDKVWAPKFDYSLLYDSKFINRRIVSLNPLVFNNDQNIHFLGRLLISHLFPTNPEFSKKVTPKVRAELLDKWVQIGCRFEHLGDMVSWLAVATIICSIPVLRSSSWKYVPDQSLKTIFKDWVPTIIQLERRQRTSKSTSSVFILAPPNLDDDFTRANVISYFGDLLIHADDLPSDTKFKYLEKKINRTKNAFHKWQQRLQAIDSTRHKTNSTENVRDNDSPNNVVYQLWKFHLSQPPLNIEGIMKLSVQHEPPIIDQKAYSTIGSQRSALVTGSYLPILFNELFPNYSLFPKNTLVGAASDAKLPPPRSSARLSKSLSISEPIPIASNSHTMGSLTDDAMSSKNDNNKVTGVGKIDGPVIKEMSSKQSNKQRLLKSVRDVFNIDMDVFHISDELVFKSVYDNDGKSRPASMVIETPKRFSQHSSMLINNPATPNQKMRDSLDTTGRLSKTLENMDFFNNIGQVSDSLKESIIRVVLKSSSLEKIFDLLVLTSNIFSKLVDTKDLENYYYHQRQRGHSTRGLSDDNIGLLDYAFVKLTMDNDIFTETFFNTYKSFTTTTTVLENMAKRYVGAKSCSVSISKILDRSDDSKMKINEDTNLVSSSLYDQNFPVWDMKVTDDENINLIYMAKIQIGAAEAILHLVKNHYSDFTDDLCNNSTLLDIIKIMEQEVSTEWPTRIANSKLQKSLPENFVIETENLLTTLTDLFHGIKSAYQKQLYRPIGVNRTQKRITDILNSFNTFSFTDLNNIIDDPSFSDDMIRSFQKLHSTNYEDILEWIYQLDNFISKKFNLVSKKDWIVLFQELELLSKESLVSFFNYPLHFKSSKLINPGYLQLHEFEISNLFTWISTLILKDDNGTESLFFEKLPQSIKLLIKLHTSLTTFFVMEISNVNKSSSERLTTCKVILQILNYIRWKNGSLDLFDSEEDESPHAICPHIPAFIETAIAHAIISPESRNYELSWIKASEKLSDPTKGTQNLRSISNVLEKIDDIHIKRFIEIDDVFSKNCKNLCPCPGWFISRLLEISQFVPNMSITNSKLINFDKRRFVNNIISNVLDLIPNEREFPLDIEMSDENPSKRTTFGRILFNNFEDVNKVYRKKTKKVSESEAISERFQEQGVFNEILVNEIEKIKREARKLEVLLDQEKILKNSAALHQAVPKKNRKSVIISGTHSDNDHSYNINKNTGQTPSLGSVMESNNSARNRRDSRASFSTNRSSVVSNSSHNGVSKKIGGFFRRPFSIGGFNTSSSNYSLNSILSQEVSSNKSILPSILPEVDSMQLHDLKPSYSLKTFEIKSIMEIINHRNIPAYYYAFKIVMQNGHEYLIQTASSSDLTEWIKMIKASKRFSFHSKKYKGKTHNKIFGVPLEDVCERENTLIPTIVVKLLEEIELRGLDEVGLYRIPGSIGSINALKNAFDEEGATDNSFTLEDDRWFEVNAIAGCFKMYLRELPDSLFSHAMVNDFTDLAIKYKAHAMVNEEYKRMMNELLQKLPTCYYQTLKRIVFHLNKVHQHVVNNKMDASNLAIVFSMSFINQEDLANSMGSRLGAVQTILQDFIKNPNDYFKQ

Target ORF information:

RefSeq Version	NM_001179045.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Bem2p (BEM2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179045.3

ORF Insert Sequence:

ATGAAAGGTC TTCTCTGGTC TAAGAACAGG AAATCTTCAA CGGCCAGTGC TAGTTCAAGC 
TCAACTTCCA CGTCTCATAA GACAACTACT GCGTCTACAG CATCGTCGTC GTCGCCGTCA 
TCATCCTCTC AAACTATTCG AAACTCGACA AGCGGTGCTT CTCCATACAT GCATTCCCAC 
CATCACCATG GCCAGGGACA CAGTCACCAC AGAGGGGAAG ACAACAACAG AGATAAGAGA 
AAAAGCTCCG TATTTCCACC CTCCAAGCAG TACACATCCA CTAGTTCCAG TCAGGTGAAC 
TTGGGGATGT ATCATTCGGA TACTAACACC AGATCTTCTA GAAGCATAGC CTCTACGCTA 
AAAGACGATT CTCCGTCTGT CTGCTCCGAA GATGAAATAA GCAATTCTTC TAGCCAGAAG 
TCCAATGCCC AGGATGAGAC TCCCATTGCG TACAAGAAGA GTGCCCATTC GAAAGACTCA 
TTGTTGCCTT CACGTTCCTC GTCTTTATCA CCTCCTCAGT CTCGATGTAG CACTGGAACT 
ACGCTAGAGA AATCGTTGAA CACTTCAGGT ATCAGTAACA GTAGCGGCAC TAATAATAAC 
AATAGCAATA ATAACAACGA TAACGAGCAA AAGCAAAGAA ATGTTATCCA CTTAAACTCT 
GAAAACTATG ATACAACTGT TTTTAAGACT GGATGGGTTA ATAAGTCCCA TGGCCAGACT 
GTTGCTACAA ACTATAATAG CTCAATGACA GCCCCTTCCT CTTCCTCTTC GTCCTCCTCT 
CAGAATCTCC GTAATGATGC GTATTCTAGA AACAGGGAAA GCAGGTTTTA TGGGAACGAC 
GGTTCGTCTT TAAAAAATGA TGACAGCAGT AGTACTACGG CCACTAACAG TGGCAATGAT 
GTGGCCTCAG CAAGAAGCAG CATGGCTATA GATCCTCAAA TGCTAGTACC TGATTACCGT 
CTTTATAGGG CTCAATTGAA AGGTTGCGTT CTCAACCTTT ACAAAAGTGG TTTGAATAGT 
AACATTAAGT TTTTTGATCC AACTTTACCA GCTTCAAATA GTAGTATAGC AAATGAAAAC 
CATCAACAGA AAAAACAACA AACAAACAAC CAAGCACAAG CGGAAGCTCT GCACCAAAAG 
CAATCATTTG GCCAAATGGG TGAACCTATT ACACTGGACC TGAAGTATTT ATCTGAAGTT 
TACCCGCACC CAGATTTAAG ACAAGACAGT GATGGTAAAA TTATATCAGG CACAATAGAA 
AGTTTATGTC ACACAGTCTT ATTTTACCCC GGTCCAAAGC AATCGGACGT ACCTAATGAG 
AAATCATTAT CTAAGACACA TAGAGCTGTT ATTAATCTGT TATTGATGTT TCCATTACTA 
GATCATTTCA TTAAGTTTCT AAAGGTATTC AACCAATTCG GTCTTTCTTT TACCAAGAAT 
AAATCAAGAT TGACAAATAA TTCCACACAA TTCTACAACA TATCCCCTGC TGTCGATGAC 
TCCATGACTC AAAGATTAGC TTTAACTGCT AAAACAATCC TTGATGTATT TCCGGGATTT 
TTGCTGGATG AACCAATGCT GAAGACTATA ATTTCCTTAT TAGATACTAT ATCTTTGCAT 
AACGATGAAA TTTCCAACAA TTTGAAAATT AAAATCGCCA ATAAACATAA CGAATTAATG 
AAACTTACCG CATTCACTCG ATCTCTTCCA ATGGCAACAT CATCTACACA TGAATTAGAG 
ATTATATTGG ATCCAAGTCA TTTCCTGTCT TTAGACATTA CAACATTGGC TGATGAAGTT 
CATCATATCA ATTTAAAGTT TGATAAAGTA TGGGCACCGA AATTTGACTA TTCTCTGCTG 
TATGATTCGA AGTTTATTAA TCGAAGAATT GTCTCGTTAA ATCCATTGGT ATTCAACAAC 
GACCAAAATA TCCATTTTCT TGGAAGGCTA CTAATATCAC ATTTATTTCC AACAAATCCT 
GAGTTTAGTA AAAAAGTGAC TCCAAAAGTG AGAGCTGAGT TATTAGATAA ATGGGTCCAA 
ATTGGCTGTA GATTTGAACA TTTAGGAGAC ATGGTATCAT GGCTAGCTGT CGCCACTATC 
ATTTGTTCAA TTCCTGTACT AAGGTCATCT TCATGGAAAT ATGTTCCTGA TCAGTCATTG 
AAAACTATTT TTAAAGATTG GGTTCCTACC ATTATTCAGT TAGAGAGGCG ACAAAGGACA 
TCTAAATCTA CAAGTAGTGT TTTCATACTC GCTCCTCCAA ATCTGGATGA CGACTTTACG 
AGAGCTAACG TGATTTCTTA TTTTGGCGAT TTATTAATTC ATGCTGATGA TTTACCATCT 
GATACGAAAT TCAAGTACTT GGAAAAGAAA ATTAACAGAA CAAAAAACGC TTTTCATAAA 
TGGCAACAAA GATTACAAGC AATCGACTCT ACGAGACATA AAACTAATTC GACAGAAAAC 
GTCAGGGATA ATGATTCTCC TAATAACGTT GTTTATCAGC TCTGGAAATT TCATTTATCA 
CAACCTCCGT TGAACATTGA AGGAATAATG AAATTAAGTG TCCAACACGA ACCTCCAATA 
ATCGACCAGA AAGCGTATTC TACTATTGGT TCTCAAAGGA GTGCTTTAGT AACTGGAAGT 
TATTTACCCA TTTTATTCAA TGAACTATTC CCAAATTATT CACTATTTCC TAAGAATACA 
TTAGTGGGTG CTGCCAGTGA TGCGAAGTTG CCGCCTCCAA GATCATCCGC GCGCCTCTCA 
AAATCACTTT CAATCAGTGA ACCAATTCCA ATAGCTTCAA ACAGTCACAC TATGGGCTCA 
TTAACAGATG ATGCTATGTC CTCAAAAAAT GATAATAATA AAGTTACAGG TGTTGGAAAA 
ATTGACGGAC CTGTGATCAA AGAGATGTCG TCAAAACAAT CAAATAAACA GAGACTATTA 
AAATCTGTGA GAGATGTTTT TAATATTGAC ATGGATGTGT TTCATATATC AGATGAGTTA 
GTATTTAAAT CGGTATACGA TAATGATGGT AAATCCCGTC CTGCTAGTAT GGTCATAGAA 
ACACCAAAGA GATTCTCACA ACACTCTTCC ATGCTTATTA ATAATCCAGC TACTCCAAAC 
CAAAAGATGA GAGATTCCTT GGATACCACT GGAAGACTAA GTAAGACATT AGAAAATATG 
GACTTTTTCA ACAATATTGG TCAAGTATCA GATTCACTAA AAGAATCGAT TATTCGTGTC 
GTATTGAAAT CGTCGTCGCT AGAAAAGATT TTTGATTTAC TGGTATTAAC TTCAAATATC 
TTTTCAAAAT TGGTAGACAC TAAAGACCTT GAGAATTATT ACTATCATCA AAGACAAAGG 
GGTCATTCTA CCAGAGGGCT TTCCGATGAT AACATAGGAC TACTAGATTA TGCATTCGTT 
AAACTAACTA TGGACAATGA TATTTTCACA GAAACATTCT TCAACACTTA TAAAAGCTTT 
ACTACTACAA CTACGGTATT AGAAAATATG GCAAAACGGT ATGTTGGTGC AAAGAGTTGC 
AGCGTGTCAA TTTCCAAGAT TTTAGATCGC AGTGATGATT CTAAAATGAA GATAAATGAG 
GATACAAATT TAGTGTCCTC TTCATTATAC GACCAAAATT TTCCTGTTTG GGATATGAAA 
GTAACTGACG ATGAAAACAT AAACCTTATT TATATGGCAA AAATCCAAAT CGGCGCCGCG 
GAAGCTATAC TACATTTAGT GAAAAATCAT TACTCTGACT TTACTGACGA TTTATGTAAC 
AACTCTACTC TATTGGATAT TATAAAAATA ATGGAACAAG AAGTATCCAC TGAATGGCCT 
ACGCGAATTG CAAACTCAAA GTTACAAAAA AGCCTACCAG AAAATTTTGT GATTGAAACA 
GAAAATTTGT TGACTACGTT AACTGATCTA TTTCATGGTA TTAAATCAGC ATACCAAAAG 
CAACTATACA GACCTATTGG TGTCAATAGA ACTCAGAAGA GAATAACTGA CATTTTGAAT 
TCCTTTAACA CATTCTCTTT TACAGATCTT AACAACATCA TTGATGATCC TTCCTTTAGC 
GATGACATGA TTAGATCTTT TCAAAAGTTA CACTCAACCA ATTACGAAGA TATACTAGAA 
TGGATCTACC AGTTAGATAA CTTTATTTCT AAGAAGTTCA ATTTAGTTTC TAAGAAAGAT 
TGGATAGTTC TTTTCCAGGA GTTGGAATTG CTTTCAAAGG AATCTCTGGT CTCTTTTTTC 
AATTATCCGC TACATTTTAA GTCAAGCAAA TTGATAAACC CCGGATATTT GCAATTGCAC 
GAGTTTGAAA TTTCCAATCT TTTTACTTGG ATTTCTACAT TGATTCTTAA GGATGATAAT 
GGTACGGAGA GTCTTTTTTT TGAAAAACTA CCACAATCTA TAAAATTGTT AATAAAGTTA 
CACACGAGTC TAACTACGTT TTTCGTGATG GAAATTTCAA ACGTCAACAA AAGCAGTAGT 
GAAAGGTTAA CTACTTGTAA AGTTATATTA CAAATTTTGA ACTACATCCG CTGGAAAAAC 
GGAAGTTTGG ATTTATTTGA TTCAGAGGAG GATGAAAGTC CTCATGCTAT TTGTCCACAC 
ATTCCAGCTT TTATTGAAAC TGCTATTGCG CATGCCATAA TATCACCAGA GTCTAGAAAC 
TATGAACTTT CGTGGATCAA AGCTTCTGAA AAACTTTCGG ACCCTACTAA AGGAACCCAA 
AATCTAAGAT CGATTAGTAA CGTTTTAGAG AAGATAGATG ACATACATAT AAAGAGATTC 
ATTGAGATTG ATGATGTATT CAGCAAAAAC TGCAAGAATT TATGCCCCTG CCCTGGTTGG 
TTCATTTCAA GGTTACTAGA AATATCTCAG TTTGTCCCTA ACATGAGTAT CACTAATTCG 
AAACTTATTA ATTTTGACAA AAGGAGATTT GTTAACAACA TAATATCTAA TGTCTTGGAT 
TTAATTCCGA ACGAAAGGGA GTTTCCACTT GATATTGAAA TGAGTGACGA AAATCCTTCG 
AAAAGAACTA CATTTGGCCG CATTCTGTTC AATAATTTTG AAGATGTTAA TAAAGTATAC 
AGAAAGAAGA CTAAAAAAGT ATCAGAATCA GAAGCCATAT CGGAGAGATT TCAAGAGCAG 
GGAGTATTTA ATGAAATACT AGTCAATGAA ATTGAAAAAA TAAAGAGGGA AGCCAGAAAA 
TTAGAGGTTT TACTCGATCA AGAAAAGATC TTGAAGAACT CTGCTGCTTT ACATCAGGCC 
GTTCCGAAAA AGAATAGGAA GTCTGTTATT ATCTCAGGTA CTCATAGTGA CAACGATCAT 
AGCTATAATA TCAATAAGAA CACCGGTCAA ACGCCTTCTC TAGGTAGTGT TATGGAAAGC 
AATAATTCAG CAAGGAATAG AAGAGATTCA AGGGCCTCCT TTTCCACAAA TAGATCATCA 
GTAGTGTCAA ATTCCAGCCA TAATGGTGTT AGTAAAAAAA TAGGTGGATT TTTCAGAAGA 
CCATTTTCAA TCGGTGGATT CAACACATCC AGTTCTAATT ATTCACTCAA CAGTATATTA 
TCGCAAGAAG TTTCAAGTAA CAAATCCATT CTACCAAGTA TACTTCCAGA GGTTGATTCC 
ATGCAACTAC ACGACTTAAA ACCTAGTTAT AGTTTGAAGA CCTTTGAAAT CAAGTCAATA 
ATGGAAATAA TCAATCACAG AAACATTCCG GCTTACTACT ATGCGTTCAA GATAGTTATG 
CAAAATGGCC ATGAATATCT GATTCAAACG GCTAGTTCTA GTGACCTCAC AGAATGGATA 
AAAATGATCA AGGCTTCCAA GAGATTTTCA TTCCATTCGA AAAAATATAA AGGTAAAACT 
CATAATAAGA TATTTGGGGT ACCATTAGAA GACGTTTGTG AAAGAGAGAA TACCTTGATA 
CCTACAATTG TTGTGAAATT GCTGGAAGAG ATTGAGCTGC GTGGGCTAGA TGAAGTGGGA 
TTGTACAGAA TTCCTGGTTC CATCGGCAGT ATAAATGCAC TAAAGAACGC ATTTGACGAA 
GAAGGTGCAA CAGATAATTC CTTTACATTA GAGGACGATA GATGGTTTGA AGTGAATGCG 
ATCGCAGGCT GTTTTAAGAT GTATTTAAGA GAGTTACCAG ATTCTTTATT TTCTCATGCT 
ATGGTGAACG ACTTTACCGA TTTGGCGATT AAGTATAAAG CACATGCAAT GGTAAATGAA 
GAATACAAGA GAATGATGAA TGAGTTACTC CAAAAGTTAC CTACATGCTA TTACCAAACT 
TTGAAAAGAA TTGTATTTCA TTTGAATAAA GTACATCAGC ATGTTGTAAA TAATAAAATG 
GACGCATCAA ACTTGGCTAT TGTATTTTCC ATGAGTTTTA TAAACCAAGA AGACTTAGCC 
AACAGCATGG GGTCCAGATT AGGTGCAGTT CAGACAATCC TGCAAGATTT TATTAAGAAT 
CCAAATGATT ATTTCAAGCA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome V.
Nature387(6632 Suppl)78-81(1997 May)
Dietrich FS,Mulligan J,Hennessy K,Yelton MA,Allen E,Araujo R,Aviles E,Berno A,Brennan T,Carpenter J,Chen E,Cherry JM,Chung E,Duncan M,Guzman E,Hartzell G,Hunicke-Smith S,Hyman RW,Kayser A,Komp C,Lashkari D,Lew H,Lin D,Mosedale D,Davis RW

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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BEM2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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