Gene Symbol | RPN1 |
Entrez Gene ID | 856422 |
Full Name | proteasome regulatory particle base subunit RPN1 |
Synonyms | HRD2,NAS1 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
ORF » Species Summary » Saccharomyces cerevisiae S288C » RPN1 cDNA ORF clone
Gene Symbol | RPN1 |
Entrez Gene ID | 856422 |
Full Name | proteasome regulatory particle base subunit RPN1 |
Synonyms | HRD2,NAS1 |
Gene Type | protein-coding |
Organism | Saccharomyces cerevisiae S288C |
mRNA | Protein | Name |
---|---|---|
NM_001179157.1 | NP_011892.1 | proteasome regulatory particle base subunit RPN1 |
Rattus norvegicus (Norway rat) | Psmd2 | NP_001026809.1 |
Magnaporthe oryzae (rice blast fungus) | MGG_07120 | XP_003715310.1 |
Homo sapiens (human) | PSMD2 | NP_002799.3 |
Drosophila melanogaster (fruit fly) | Rpn1 | NP_649158.1 |
Saccharomyces cerevisiae (baker's yeast) | RPN1 | NP_011892.1 |
Schizosaccharomyces pombe (fission yeast) | mts4 | NP_596171.1 |
Arabidopsis thaliana (thale cress) | RPN1B | NP_194576.5 |
Arabidopsis thaliana (thale cress) | RPN1A | NP_565477.1 |
PSMD2 | XP_001093407.2 | |
Mus musculus (house mouse) | Psmd2 | NP_598862.1 |
Kluyveromyces lactis | KLLA0E11507g | XP_454469.1 |
Eremothecium gossypii | AGOS_ACR072C | NP_983474.1 |
Oryza sativa (rice) | Os02g0146700 | NP_001045879.1 |
Pan troglodytes (chimpanzee) | PSMD2 | XP_001147451.2 |
Gallus gallus (chicken) | PSMD2 | NP_001012952.1 |
Anopheles gambiae (African malaria mosquito) | AgaP_AGAP002481 | XP_563695.1 |
Oryza sativa (rice) | Os09g0326800 | NP_001062884.1 |
Canis lupus familiaris (dog) | PSMD2 | XP_535824.3 |
Bos taurus (cattle) | PSMD2 | NP_001094667.1 |
Danio rerio (zebrafish) | psmd2 | NP_956840.1 |
Caenorhabditis elegans (roundworm) | rpn-1 | NP_501064.1 |
Neurospora crassa | NCU07721 | XP_961971.2 |
Xenopus tropicalis (tropical clawed frog) | psmd2 | NP_001015819.1 |
Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
GeneRIFs: Gene References Into Functions What's a GeneRIF?
The structures of proteasome substrate receptor complexes with the shuttle factors that deliver ubiquitinated proteins to proteasomes have been solved, namely human Rpn13 complexed with PLIC2 and Saccharomyces cerevisiae Rpn1 with Rad23.
Title: Structures of Rpn1 T1:Rad23 and hRpn13:hPLIC2 Reveal Distinct Binding Mechanisms between Substrate Receptors and Shuttle Factors of the Proteasome.
Data suggest regulated process for removal of proteasomes upon nitrogen starvation; autophagy involves separate processes for dissociation and nuclear export of core (PRE2) and regulatory (RPN1) proteins; only core protein autophagy depends on UBP3.
Title: Starvation Induces Proteasome Autophagy with Different Pathways for Core and Regulatory Particles.
Study found that the five known proteasomal ubiquitin receptors in yeast are collectively nonessential for ubiquitin recognition and identified a sixth receptor, Rpn1. A site (T1:) in the Rpn1 toroid recognized ubiquitin and ubiquitin-like (UBL:) domains of substrate shuttling factors.
Title: Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome.
Rpn1 and Rpn2 coordinate ubiquitin processing factors at proteasome.
Title: Rpn1 and Rpn2 coordinate ubiquitin processing factors at proteasome.
The current study identifies residues in the LRR1 domain of Rpn1 that contribute to shuttle receptor docking. It validates Ddi1 as a proteasomal shuttle receptor whose stable binding to the proteasome depends on Rpn1 residue D517.
Title: Identification of a functional docking site in the Rpn1 LRR domain for the UBA-UBL domain protein Ddi1.
The following RPN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
CloneID | OSi05225 | |
Clone ID Related Accession (Same CDS sequence) | NM_001179157.1 | |
Accession Version | NM_001179157.1 Latest version! | Documents for ORF clone product in default vector |
Sequence Information | ORF Nucleotide Sequence (Length: 2982bp) Protein sequence SNP |
|
Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
Clone information | Clone Map | MSDS |
Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
ORF Insert Method | CloneEZ™ Seamless cloning technology | |
Insert Structure | linear | |
Update Date | 2019-10-31 | |
Organism | Saccharomyces cerevisiae S288C | |
Product | proteasome regulatory particle base subunit RPN1 | |
Comment | Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. |
1 | ATGGTAGACG AAAGTGATAA GAAACAACAG ACTATTGATG AACAATCGCA GATAAGTCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
RefSeq | NP_011892.1 |
CDS | 1..2982 |
Translation |
Target ORF information:
Target ORF information:
|
NM_001179157.1 |
1 | ATGGTAGACG AAAGTGATAA GAAACAACAG ACTATTGATG AACAATCGCA GATAAGTCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Life with 6000 genes. |
Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII. |