YHR020W cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	YHR020W
Entrez Gene ID	856413
Full Name	proline--tRNA ligase
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179150.1	NP_011884.1	proline--tRNA ligase

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	EPRS	NP_001006398.1
Kluyveromyces lactis	KLLA0A08448g	XP_451373.1
Oryza sativa (rice)	Os12g0443700	NP_001066707.2
Schizosaccharomyces pombe (fission yeast)	prs1	NP_596162.1
Magnaporthe oryzae (rice blast fungus)	MGG_10357	XP_003719394.1
Arabidopsis thaliana (thale cress)	AT3G62120	NP_191771.1
Saccharomyces cerevisiae (baker's yeast)	YHR020W	NP_011884.1
Neurospora crassa	NCU04449	XP_955907.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

replacement of the N-terminal domain of S. cerevisiae ProRS with the Escherichia coli INS domain confers posttransfer editing function to this chimeric enzyme, with specificity for yeast Ala-tRNA(Pro).
Title: Restoring species-specific posttransfer editing activity to a synthetase with a defunct editing domain.

The following YHR020W gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YHR020W cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05217	NM_001179150.1 Latest version!	Saccharomyces cerevisiae S288C proline--tRNA ligase (YHR020W), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

YHR020W ( NM_001179150.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011884.1 Saccharomyces cerevisiae S288C proline--tRNA ligase (YHR020W), partial mRNA.

Price & Availability↑

CloneID	OSi05217
Clone ID Related Accession (Same CDS sequence)	NM_001179150.1
Accession Version	NM_001179150.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	proline--tRNA ligase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCCTGTTT CGGAAGCGTT TGCCAAGTTG TGTGTGAATG AGAAGCCTCC TGCTGAATCT 
GCCGTTGCGG TGAAGTCTTT GGTCTTCAAA CCAAAGACTC CAAAGTCCGC CACCCCTGTT 
CCTATCGTTG TGGTGGCTTT GCAATCTACT ACTACTCCTT CTGCGTTGAT TGCCAACGCT 
ACGTCCAGTA AGGATCCAAG ATTGGCTCGC GATGATTTGG TCAAGCAAGC GTTCCAATCT 
GAATCCGCTA GAGCTTTTAT CTTGGGGGAT TTGGCTAACG CCACGTCCAA TTTTCACTTG 
CTAATTGATC ACGAGTTGGG TACTGTTGAC GGTGACACTA TCTTGCAGTT AAACGACAGT 
ACTTATATGA AGAAATCTGA CATGATGAAA TTTCTAAACA ATTTTGAGGA TAGTCAAAAA 
GTGGTTGATT TCTCCCAAGA GGTGTCTAAG GAAACCGCTA CAGAAGGCAA GAAACAACAG 
AAAAAGCAGC AACCTTCTAA GGCAGGCACT GCAGCAGCAG CAGCAGCAGC AGCTTTGGAA 
GACGCCAAAC TTATTGGTAT CACCGTAGAC AAAGCGTTGG ATTTCCCAGG CTGGTACCAA 
CAAATCTTGA CCAAGGGTGA AATGTTGGAC TACTACGATG TTTCTGGCTG TTACATCTTA 
AGACCTCCTT CTTATGCGAT CTGGGAAAAC ATTCAGAAAT GGTTCGACGA CAAGATCAAG 
GCCATTGGTG TACAAAATGC CTATTTCCCA ATGTTTGTTT CCTCACGTGT GTTGGAAAAG 
GAAAAGGACC ATGTTGAAGG CTTTGCTCCA GAAGTTGCCT GGGTCACCAG AGCTGGCTCC 
TCTGAATTGG AAGAACCAAT CGCCATTAGA CCAACTTCTG AAACTGTCAT GTATCCTTAC 
TACGCAAAAT GGGTCCAGTC TTACAGAGAT TTACCATTGA AACTAAACCA ATGGAATTCT 
GTGGTTAGAT GGGAATTCAA GCATCCTCAG CCCTTTTTAA GAACCAGAGA ATTCTTGTGG 
CAAGAAGGTC ATACTGCTCA CTTGACTGCT AAGGATGCCG AAGAGGAGGT TTTGCAAATC 
TTAGATTTCT ACGCTGGGGT TTACGAAGAG TTATTAGCTG TTCCAGTGGT TAAGGGTAGA 
AAGACCGAAA AGGAAAAATT TGCTGGTGGT GACTTCACCA CAACTTGTGA AGGCTATATT 
CCACAAACCG GTCGTGGTAT TCAAGGTGCC ACCTCTCACC ATTTGGGCCA AAACTTTTCT 
AAGATGTTCA ATTTGTCTGT GGAGAACCCA CTAGGTTCTG ATCATCCAAA GATCTTTGCG 
TACCAAAATT CATGGGGTTT ATCCACTCGT GTCATTGGTG TCATGGTCAT GATCCACTCT 
GACAACAAGG GTTTGGTTAT CCCACCAAGA GTGTCCCAAT TCCAATCCGT TGTCATTCCA 
GTGGGCATCA CAAAGAAGAC CTCTGAGGAA CAGCGTAAAC ACATTCATGA AACAGCCAGA 
AGTGTGGAAT CCCGTTTGAA AAAGGTTGGC ATTAGAGCCT TTGGTGACTA TAACGATAAT 
TACACACCAG GTTGGAAATT TTCCCAATAC GAGCTAAAGG GTATTCCAAT CCGTATTGAA 
TTAGGTCCTA AGGATATTGA AAAAAACCAA GTCGTTGTCG TTCGTAGAAA TGACTCCAAG 
AAATACGTCG TTTCTTTCGA CGAATTGGAA GCCCGTATCC CAGAAATCTT AGAAGAAATG 
CAAGGAGATT TGTTCAAAAA AGCCAAGGAG CTATTCGACA CCCACAGAGT TATCGTTAAT 
GAATGGAGTG GTTTTGTTCC AGCATTGAAC AAGAAGAACG TTATCTTAGC ACCATGGTGT 
GGTGTGATGG AATGTGAAGA AGATATCAAG GAATCTTCTG CAAAGAAAGA CGATGGTGAA 
GAATTCGAGG AAGATGACAA GGCACCAAGT ATGGGTGCCA AATCTTTATG TATCCCATTC 
GACCAACCTG TATTGAATGA AGGTCAAAAA TGTATCAAGT GTGAACGTAT TGCTGTCAAT 
TACTGTATGT TCGGTCGTTC TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011884.1
CDS	1..2067
Translation	MPVSEAFAKLCVNEKPPAESAVAVKSLVFKPKTPKSATPVPIVVVALQSTTTPSALIANATSSKDPRLARDDLVKQAFQSESARAFILGDLANATSNFHLLIDHELGTVDGDTILQLNDSTYMKKSDMMKFLNNFEDSQKVVDFSQEVSKETATEGKKQQKKQQPSKAGTAAAAAAAALEDAKLIGITVDKALDFPGWYQQILTKGEMLDYYDVSGCYILRPPSYAIWENIQKWFDDKIKAIGVQNAYFPMFVSSRVLEKEKDHVEGFAPEVAWVTRAGSSELEEPIAIRPTSETVMYPYYAKWVQSYRDLPLKLNQWNSVVRWEFKHPQPFLRTREFLWQEGHTAHLTAKDAEEEVLQILDFYAGVYEELLAVPVVKGRKTEKEKFAGGDFTTTCEGYIPQTGRGIQGATSHHLGQNFSKMFNLSVENPLGSDHPKIFAYQNSWGLSTRVIGVMVMIHSDNKGLVIPPRVSQFQSVVIPVGITKKTSEEQRKHIHETARSVESRLKKVGIRAFGDYNDNYTPGWKFSQYELKGIPIRIELGPKDIEKNQVVVVRRNDSKKYVVSFDELEARIPEILEEMQGDLFKKAKELFDTHRVIVNEWSGFVPALNKKNVILAPWCGVMECEEDIKESSAKKDDGEEFEEDDKAPSMGAKSLCIPFDQPVLNEGQKCIKCERIAVNYCMFGRSY

Target ORF information:

RefSeq Version	NM_001179150.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C proline--tRNA ligase (YHR020W), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179150.1

ORF Insert Sequence:

ATGCCTGTTT CGGAAGCGTT TGCCAAGTTG TGTGTGAATG AGAAGCCTCC TGCTGAATCT 
GCCGTTGCGG TGAAGTCTTT GGTCTTCAAA CCAAAGACTC CAAAGTCCGC CACCCCTGTT 
CCTATCGTTG TGGTGGCTTT GCAATCTACT ACTACTCCTT CTGCGTTGAT TGCCAACGCT 
ACGTCCAGTA AGGATCCAAG ATTGGCTCGC GATGATTTGG TCAAGCAAGC GTTCCAATCT 
GAATCCGCTA GAGCTTTTAT CTTGGGGGAT TTGGCTAACG CCACGTCCAA TTTTCACTTG 
CTAATTGATC ACGAGTTGGG TACTGTTGAC GGTGACACTA TCTTGCAGTT AAACGACAGT 
ACTTATATGA AGAAATCTGA CATGATGAAA TTTCTAAACA ATTTTGAGGA TAGTCAAAAA 
GTGGTTGATT TCTCCCAAGA GGTGTCTAAG GAAACCGCTA CAGAAGGCAA GAAACAACAG 
AAAAAGCAGC AACCTTCTAA GGCAGGCACT GCAGCAGCAG CAGCAGCAGC AGCTTTGGAA 
GACGCCAAAC TTATTGGTAT CACCGTAGAC AAAGCGTTGG ATTTCCCAGG CTGGTACCAA 
CAAATCTTGA CCAAGGGTGA AATGTTGGAC TACTACGATG TTTCTGGCTG TTACATCTTA 
AGACCTCCTT CTTATGCGAT CTGGGAAAAC ATTCAGAAAT GGTTCGACGA CAAGATCAAG 
GCCATTGGTG TACAAAATGC CTATTTCCCA ATGTTTGTTT CCTCACGTGT GTTGGAAAAG 
GAAAAGGACC ATGTTGAAGG CTTTGCTCCA GAAGTTGCCT GGGTCACCAG AGCTGGCTCC 
TCTGAATTGG AAGAACCAAT CGCCATTAGA CCAACTTCTG AAACTGTCAT GTATCCTTAC 
TACGCAAAAT GGGTCCAGTC TTACAGAGAT TTACCATTGA AACTAAACCA ATGGAATTCT 
GTGGTTAGAT GGGAATTCAA GCATCCTCAG CCCTTTTTAA GAACCAGAGA ATTCTTGTGG 
CAAGAAGGTC ATACTGCTCA CTTGACTGCT AAGGATGCCG AAGAGGAGGT TTTGCAAATC 
TTAGATTTCT ACGCTGGGGT TTACGAAGAG TTATTAGCTG TTCCAGTGGT TAAGGGTAGA 
AAGACCGAAA AGGAAAAATT TGCTGGTGGT GACTTCACCA CAACTTGTGA AGGCTATATT 
CCACAAACCG GTCGTGGTAT TCAAGGTGCC ACCTCTCACC ATTTGGGCCA AAACTTTTCT 
AAGATGTTCA ATTTGTCTGT GGAGAACCCA CTAGGTTCTG ATCATCCAAA GATCTTTGCG 
TACCAAAATT CATGGGGTTT ATCCACTCGT GTCATTGGTG TCATGGTCAT GATCCACTCT 
GACAACAAGG GTTTGGTTAT CCCACCAAGA GTGTCCCAAT TCCAATCCGT TGTCATTCCA 
GTGGGCATCA CAAAGAAGAC CTCTGAGGAA CAGCGTAAAC ACATTCATGA AACAGCCAGA 
AGTGTGGAAT CCCGTTTGAA AAAGGTTGGC ATTAGAGCCT TTGGTGACTA TAACGATAAT 
TACACACCAG GTTGGAAATT TTCCCAATAC GAGCTAAAGG GTATTCCAAT CCGTATTGAA 
TTAGGTCCTA AGGATATTGA AAAAAACCAA GTCGTTGTCG TTCGTAGAAA TGACTCCAAG 
AAATACGTCG TTTCTTTCGA CGAATTGGAA GCCCGTATCC CAGAAATCTT AGAAGAAATG 
CAAGGAGATT TGTTCAAAAA AGCCAAGGAG CTATTCGACA CCCACAGAGT TATCGTTAAT 
GAATGGAGTG GTTTTGTTCC AGCATTGAAC AAGAAGAACG TTATCTTAGC ACCATGGTGT 
GGTGTGATGG AATGTGAAGA AGATATCAAG GAATCTTCTG CAAAGAAAGA CGATGGTGAA 
GAATTCGAGG AAGATGACAA GGCACCAAGT ATGGGTGCCA AATCTTTATG TATCCCATTC 
GACCAACCTG TATTGAATGA AGGTCAAAAA TGTATCAAGT GTGAACGTAT TGCTGTCAAT 
TACTGTATGT TCGGTCGTTC TTATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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YHR020W cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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