GPA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	GPA1
Entrez Gene ID	856394
Full Name	guanine nucleotide-binding protein subunit alpha
Synonyms	CDC70,DAC1,SCG1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179135.1	NP_011868.1	guanine nucleotide-binding protein subunit alpha

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	GNAI2	NP_990733.1
Canis lupus familiaris (dog)	GNAI2	NP_001003364.1
Mus musculus (house mouse)	Gnai2	NP_032164.2
Danio rerio (zebrafish)	gnai2b	NP_956136.1
Saccharomyces cerevisiae (baker's yeast)	GPA1	NP_011868.1
Xenopus tropicalis (tropical clawed frog)	gnai2	NP_989250.1
Homo sapiens (human)	GNAI2	NP_002061.1
Pan troglodytes (chimpanzee)	GNAI2	XP_001167942.1
	GNAI2	XP_001103122.1
Bos taurus (cattle)	GNAI2	NP_001094510.1
Magnaporthe oryzae (rice blast fungus)	MGG_00365	XP_003718715.1
Rattus norvegicus (Norway rat)	Gnai2	NP_112297.1
Kluyveromyces lactis	KLLA0F25916g	XP_456234.1
Eremothecium gossypii	AGOS_ADR153C	NP_984250.1
Neurospora crassa	NCU06493	XP_957133.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Johnston M, Andrews S, Brinkman R, Cooper J, Ding H, Dover J, Du Z, Favello A, Fulton L, Gattung S
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A mutant gpa1 protein is biochemically different and still physiologically relevant.
Title: Soya bean G? proteins with distinct biochemical properties exhibit differential ability to complement Saccharomyces cerevisiae gpa1 mutant.

Data indicate that proper endocytosis of G protein alpha subunit Gpa1 is required for sustained morphogenesis and efficient mating.
Title: Guanine nucleotide-binding protein (G?) endocytosis by a cascade of ubiquitin binding domain proteins is required for sustained morphogenesis and proper mating in yeast.

the same kinases and phosphatase that regulate Snf1 also regulate Gpa1.
Title: Regulation of yeast G protein signaling by the kinases that activate the AMPK homolog Snf1.

Gpa1 serves as a positional determinant for Kar3-bound microtubule plus ends during mating.
Title: The mating-specific Galpha interacts with a kinesin-14 and regulates pheromone-induced nuclear migration in budding yeast.

the ubiquitin ligase Rsp5 is necessary for Gpa1 mono-ubiquitination in vivo and that the purified enzyme is sufficient to catalyze Gpa1 mono-ubiquitination in vitro.
Title: G Protein Mono-ubiquitination by the Rsp5 Ubiquitin Ligase.

The following GPA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05201	NM_001179135.1 Latest version!	Saccharomyces cerevisiae S288C guanine nucleotide-binding protein subunit alpha (GPA1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPA1 ( NM_001179135.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011868.1 Saccharomyces cerevisiae S288C guanine nucleotide-binding protein subunit alpha (GPA1), partial mRNA.

Price & Availability↑

CloneID	OSi05201
Clone ID Related Accession (Same CDS sequence)	NM_001179135.1
Accession Version	NM_001179135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	guanine nucleotide-binding protein subunit alpha
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001140). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Veresion :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGGGTGTA CAGTGAGTAC GCAAACAATA GGAGACGAAA GTGATCCTTT TCTACAGAAC 
AAAAGAGCCA ATGATGTCAT CGAGCAATCG TTGCAGCTGG AGAAACAACG TGACAAGAAT 
GAAATAAAAC TGTTACTATT AGGTGCCGGT GAGTCAGGTA AATCAACGGT TTTAAAACAA 
TTAAAATTAT TACATCAAGG CGGTTTCTCC CATCAAGAAA GGTTACAGTA TGCTCAAGTG 
ATATGGGCAG ATGCCATACA ATCAATGAAA ATTTTGATTA TTCAGGCCAG AAAACTAGGT 
ATTCAACTTG ACTGTGATGA TCCGATCAAC AATAAAGATT TGTTTGCATG CAAGAGAATA 
CTGCTAAAGG CTAAAGCTTT AGATTATATC AACGCCAGTG TTGCCGGTGG TTCTGATTTT 
CTAAATGATT ATGTACTGAA GTACTCAGAA AGGTATGAAA CTAGGAGGCG TGTTCAGAGT 
ACCGGACGAG CAAAAGCTGC TTTCGATGAA GACGGAAATA TTTCTAATGT CAAAAGTGAC 
ACTGACAGAG ATGCTGAAAC GGTGACGCAA AATGAGGATG CTGATAGAAA CAACAGTAGT 
AGAATTAACC TACAGGATAT TTGCAAGGAC TTGAACCAAG AAGGCGATGA CCAGATGTTT 
GTTAGAAAAA CATCAAGGGA AATTCAAGGA CAAAATAGAC GAAATCTTAT TCACGAAGAC 
ATTGCTAAGG CAATAAAGCA ACTTTGGAAT AACGACAAAG GTATAAAGCA GTGTTTTGCA 
CGTTCTAATG AGTTTCAATT GGAGGGCTCA GCTGCATACT ACTTTGATAA CATTGAGAAA 
TTTGCTAGTC CGAATTATGT CTGTACGGAT GAAGACATTT TGAAGGGCCG TATAAAGACT 
ACAGGCATTA CAGAAACCGA ATTTAACATC GGCTCGTCCA AATTCAAGGT TCTCGACGCT 
GGTGGGCAGC GTTCTGAACG TAAGAAGTGG ATTCATTGTT TCGAAGGAAT TACAGCAGTT 
TTATTTGTTT TAGCAATGAG TGAATACGAC CAGATGTTGT TTGAGGATGA AAGAGTGAAC 
AGAATGCATG AATCAATAAT GCTATTTGAC ACGTTATTGA ACTCTAAGTG GTTCAAAGAT 
ACACCGTTTA TTTTGTTTTT AAATAAAATT GATTTGTTCG AGGAAAAGGT AAAAAGCATG 
CCCATAAGAA AGTACTTTCC TGATTACCAG GGACGTGTCG GCGATGCAGA AGCGGGTCTA 
AAATATTTTG AGAAGATATT TTTGAGCTTG AATAAGACAA ACAAACCAAT CTACGTGAAA 
CGAACCTGCG CTACCGATAC CCAAACTATG AAGTTCGTAT TGAGTGCAGT CACCGATCTA 
ATCATCCAGC AAAACCTTAA AAAAATTGGT ATTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_011868.1
CDS	1..1419
Translation	MGCTVSTQTIGDESDPFLQNKRANDVIEQSLQLEKQRDKNEIKLLLLGAGESGKSTVLKQLKLLHQGGFSHQERLQYAQVIWADAIQSMKILIIQARKLGIQLDCDDPINNKDLFACKRILLKAKALDYINASVAGGSDFLNDYVLKYSERYETRRRVQSTGRAKAAFDEDGNISNVKSDTDRDAETVTQNEDADRNNSSRINLQDICKDLNQEGDDQMFVRKTSREIQGQNRRNLIHEDIAKAIKQLWNNDKGIKQCFARSNEFQLEGSAAYYFDNIEKFASPNYVCTDEDILKGRIKTTGITETEFNIGSSKFKVLDAGGQRSERKKWIHCFEGITAVLFVLAMSEYDQMLFEDERVNRMHESIMLFDTLLNSKWFKDTPFILFLNKIDLFEEKVKSMPIRKYFPDYQGRVGDAEAGLKYFEKIFLSLNKTNKPIYVKRTCATDTQTMKFVLSAVTDLIIQQNLKKIGII

Target ORF information:

RefSeq Version	NM_001179135.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C guanine nucleotide-binding protein subunit alpha (GPA1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179135.1

ORF Insert Sequence:

ATGGGGTGTA CAGTGAGTAC GCAAACAATA GGAGACGAAA GTGATCCTTT TCTACAGAAC 
AAAAGAGCCA ATGATGTCAT CGAGCAATCG TTGCAGCTGG AGAAACAACG TGACAAGAAT 
GAAATAAAAC TGTTACTATT AGGTGCCGGT GAGTCAGGTA AATCAACGGT TTTAAAACAA 
TTAAAATTAT TACATCAAGG CGGTTTCTCC CATCAAGAAA GGTTACAGTA TGCTCAAGTG 
ATATGGGCAG ATGCCATACA ATCAATGAAA ATTTTGATTA TTCAGGCCAG AAAACTAGGT 
ATTCAACTTG ACTGTGATGA TCCGATCAAC AATAAAGATT TGTTTGCATG CAAGAGAATA 
CTGCTAAAGG CTAAAGCTTT AGATTATATC AACGCCAGTG TTGCCGGTGG TTCTGATTTT 
CTAAATGATT ATGTACTGAA GTACTCAGAA AGGTATGAAA CTAGGAGGCG TGTTCAGAGT 
ACCGGACGAG CAAAAGCTGC TTTCGATGAA GACGGAAATA TTTCTAATGT CAAAAGTGAC 
ACTGACAGAG ATGCTGAAAC GGTGACGCAA AATGAGGATG CTGATAGAAA CAACAGTAGT 
AGAATTAACC TACAGGATAT TTGCAAGGAC TTGAACCAAG AAGGCGATGA CCAGATGTTT 
GTTAGAAAAA CATCAAGGGA AATTCAAGGA CAAAATAGAC GAAATCTTAT TCACGAAGAC 
ATTGCTAAGG CAATAAAGCA ACTTTGGAAT AACGACAAAG GTATAAAGCA GTGTTTTGCA 
CGTTCTAATG AGTTTCAATT GGAGGGCTCA GCTGCATACT ACTTTGATAA CATTGAGAAA 
TTTGCTAGTC CGAATTATGT CTGTACGGAT GAAGACATTT TGAAGGGCCG TATAAAGACT 
ACAGGCATTA CAGAAACCGA ATTTAACATC GGCTCGTCCA AATTCAAGGT TCTCGACGCT 
GGTGGGCAGC GTTCTGAACG TAAGAAGTGG ATTCATTGTT TCGAAGGAAT TACAGCAGTT 
TTATTTGTTT TAGCAATGAG TGAATACGAC CAGATGTTGT TTGAGGATGA AAGAGTGAAC 
AGAATGCATG AATCAATAAT GCTATTTGAC ACGTTATTGA ACTCTAAGTG GTTCAAAGAT 
ACACCGTTTA TTTTGTTTTT AAATAAAATT GATTTGTTCG AGGAAAAGGT AAAAAGCATG 
CCCATAAGAA AGTACTTTCC TGATTACCAG GGACGTGTCG GCGATGCAGA AGCGGGTCTA 
AAATATTTTG AGAAGATATT TTTGAGCTTG AATAAGACAA ACAAACCAAT CTACGTGAAA 
CGAACCTGCG CTACCGATAC CCAAACTATG AAGTTCGTAT TGAGTGCAGT CACCGATCTA 
ATCATCCAGC AAAACCTTAA AAAAATTGGT ATTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII.
Science (New York, N.Y.)265(5181)2077-82(1994 Sep)
Johnston M,Andrews S,Brinkman R,Cooper J,Ding H,Dover J,Du Z,Favello A,Fulton L,Gattung S

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GPA1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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