CTF4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	CTF4
Entrez Gene ID	856254
Full Name	chromatin-binding protein CTF4
Synonyms	CHL15,POB1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001184232.1	NP_015461.1	chromatin-binding protein CTF4

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0B11099g	XP_452023.1
Magnaporthe oryzae (rice blast fungus)	MGG_06969	XP_003709714.1
Neurospora crassa	NCU08484	XP_963155.1
Eremothecium gossypii	AGOS_AAR103C	NP_982644.1
Saccharomyces cerevisiae (baker's yeast)	CTF4	NP_015461.1
Schizosaccharomyces pombe (fission yeast)	mcl1	NP_594128.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Bussey H, Storms RK, Ahmed A, Albermann K, Allen E, Ansorge W, Araujo R, Aparicio A, Barrell B, Badcock K, Benes V, Botstein D, Bowman S, Br?ckner M, Carpenter J, Cherry JM, Chung E, Churcher C, Coster F, Davis K, Davis RW, Dietrich FS, Delius H, DiPaolo T, Hani J
Nature387(6632 Suppl)103-5(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ctf4 as a key regulator of DNA double strand end resection at arrested forks. End resection also occurs during physiological rDNA amplification even in the presence of Ctf4. Suppression of end resection is thus important for protecting DSBs at arrested forks from chromosome rearrangements.
Title: Ctf4 Prevents Genome Rearrangements by Suppressing DNA Double-Strand Break Formation and Its End Resection at Arrested Replication Forks.

Ctf4-dependent recruitment of Dna2/Tof2 couples other processes to DNA synthesis, including rDNA copy-number regulation.
Title: Ctf4 Is a Hub in the Eukaryotic Replisome that Links Multiple CIP-Box Proteins to the CMG Helicase.

The Chl1 helicase facilitates replication fork progression under conditions of nucleotide depletion, partly independently of Ctf4 interaction.
Title: Ctf4 Links DNA Replication with Sister Chromatid Cohesion Establishment by Recruiting the Chl1 Helicase to the Replisome.

Ctf4 is an essential member of the H3 lysine 56 acetylation pathway.
Title: Replisome function during replicative stress is modulated by histone h3 lysine 56 acetylation through Ctf4.

Data indicate that a defective DNA polymerase alpha (Polalpha)/DNA primase/chromatin-binding protein Ctf4 complex causes unscheduled strand annealing and fork reversal.
Title: Error-free DNA damage tolerance and sister chromatid proximity during DNA replication rely on the Pol?/Primase/Ctf4 Complex.

The following CTF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CTF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi05082	NM_001184232.1 Latest version!	Saccharomyces cerevisiae S288C chromatin-binding protein CTF4 (CTF4), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CTF4 ( NM_001184232.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_015461.1 Saccharomyces cerevisiae S288C chromatin-binding protein CTF4 (CTF4), partial mRNA.

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CloneID	OSi05082
Clone ID Related Accession (Same CDS sequence)	NM_001184232.1
Accession Version	NM_001184232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2784bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	chromatin-binding protein CTF4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001148). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGTTTCAG TTATAGACAA GCTTGTTTTT GACTTTGGTG GGAAAACTCT GGTCTCTCTC 
GCACCAGATA ATAATACTTT GTGTGTAGCC AATAAAAATG GCTTAACCAA GATTCTGAAG 
ACAAATAACC CAGAAGAAGA GCCAGAGACT TTAGATTCTT CCAAATTGGT CTCGTCTATA 
AAATGCTATT CAAACTCGCA CTTTCTGATG ACTACAATGC AAGGTGATGC TCTTAGGTAT 
AACATCGATT CTAGTCAAGA AGAATTATTG GCTAGATTTG CTTTACCCCT ACGTGACTGC 
TGTGTTATTC ATTCAGGTAA AATGGCCGTA TTTGGAGGAG ACGACTTAGA ACTAATCCTT 
TTGGAATTGG ACGATGAAAC ACACAAAAAA CATGCCATTA AAATCGATGA ACAAGTTTCT 
CAAATTTCTT ACAATTCACA GATGAATATT TTAGCAGTTT CAATGATAAA TGGTAAGGTA 
CAAATTTTTT CTCTGACATC TACTATTCCA AACAAAGTTC ATGAGTTAAA TGATTACATA 
GTGGCCAATT CATATGATGA TACACACAGA GATAAGATAC TCTCGAATAT GATGGATGAT 
ATAGATAAAG ACAATGATAA TGACCTGAGT GAAACGGCTG ATCCAGATGA GAACAATGTA 
GCTGATCCAG AATTCTGTGC TGCTAATAGA ATTTGCACGA GAGTGGCTTG GCATCCGAAG 
GGTCTGCATT TTGCGCTACC ATGTGCAGAT GATACAGTAA AAATATTCTC TATAAAGGGA 
TATTCCCTAC AAAAGACGTT GTCCACAAAT CTCTCATCAA CAAAGGCTCA TTTCATTGAT 
TTGCAATTTG ACCCGTTACG TGGAACTTAC ATTGCGGCAG TAGATTTAAA TAATAAGTTA 
ACGGTATGGA ATTGGGAAAC TTCCGAGATC CACTACACCA GGGAATTCAA AAGAAAAATT 
ACTAATATTG CCTGGAAAAT CCAAGCGGAC TCAAAAACTC TAGACCTTGT TTTAGGTACC 
TGGTCCGGTA GTATAGCCAT TGTCCAAAAT TTGGCAGAGT CCGTAGTATC TAATATACCT 
GACCAATCTG TTGCTGAATC TTCAACTAAA CATGGGCTTT TTGTAGACTC CGAATCTGAC 
TTGGAAAACT TAGAGGGAAA TGATGATATA AACAAAAGCG ATAAGCTATT TTCAGATATT 
ACTCAAGAAG CGAATGCGGA AGATGTGTTC ACTCAAACAC ACGACGGCCC CAGTGGATTA 
AGTGAAAAGA GAAAATACAA CTTCGAAGAT GAAGAAGACT TTATTGATGA TGACGATGGT 
GCTGGTTATA TTAGTGGCAA AAAACCACAT AATGAACATT CTTATTCGAG AGTACACAAG 
ACTCATTCGT TTCCAATCAG TTTGGCAAAC ACAGGAAAAT TTCGTTATAT GCCTTTTTCT 
CCAGCGGGAA CACCTTTTGG CTTTACTGAC AGGCGTTATT TGACAATGAA TGAAGTGGGC 
TACGTATCTA CTGTCAAGAA TAGTGAGCAA TACAGCATAA CTGTCTCTTT TTTTGATGTT 
GGACGTTTTA GAGAATACCA TTTCGAGGAC TTATTTGGCT ACGATTTATG CTTCCTAAAT 
GAAAAAGGCA CTTTATTTGG CCAATCCAAA ACTGGGCAGA TACAATATAG GCCACACGAT 
AGCATACATT CAAACTGGAC CAAGATTATT CCTTTGCAAG CTGGTGAGAG AATAACAAGT 
GTGGCAGCCA CCCCGGTTCG CGTTATTGTT GGTACATCAT TAGGCTATTT CAGAAGTTTC 
AATCAATTCG GGGTTCCATT TGCTGTTGAA AAGACATCCC CAATTGTAGC GCTTACTGCT 
CAGAATTATA GGGTTTTTTC AGTACATTAT TCGCAGTTTC ATGGCCTTTC ATACTCTTTA 
TCTGAATTGG GTACTTCTAG TAAAAGGTAC TATAAAAGAG AGTGTCCACT TCCAATGAGT 
TTACCAAACA TTAATTCTGA TATGAAAAAA GACGCAAATC TTGACTACTA CAATTTTAAT 
CCGATGGGCA TCAAAAGTTT GTTCTTTTCA AGCTACGGAG ATCCATGCAT TTTTGGGTCC 
GACAACACGC TTCTATTGTT ATCAAAGTGG AGATCACCAG AAGAAAGTAA ATGGCTCCCT 
ATTCTAGATA GCAACATGGA AATATGGAAG ATGTCAGGAG GGAAGGAAAC GACAGATATA 
CATGTCTGGC CCTTGGCTTT GGCGTATGAC ACATTGAATT GTATCTTAGT TAAGGGCAAG 
CATATATGGC CCGAGTTTCC CCTTCCGTTG CCATCCGAAA TGGAGATTAG AATGCCAGTA 
TTTGTTAAGA GTAAATTACT AGAGGAAAAC AAAGCTATAT TAAATAAAAA GAACGAAATT 
GGAGCTGACA CCGAAGCGGA AGAAGGGGAA GAAGACAAAG AAATACAGAT TCCTGTTTCT 
ATGGCGGCGG AAGAAGAGTA TCTGCGCAGC AAGGTTTTGT CAGAGCTGTT GACAGATACA 
CTCGAAAATG ACGGTGAAAT GTACGGCAAC GAAAATGAGG TATTGGCAGC ATTGAACGGT 
GCATATGATA AGGCTTTGTT ACGTTTATTT GCGTCTGCAT GCTCAGACCA AAATGTTGAA 
AAGGCTCTTT CGCTTGCTCA TGAATTAAAA CAAGATAGAG CACTTACTGC AGCCGTTAAG 
ATATCAGAAA GAGCTGAGCT GCCCTCTCTC GTTAAAAAAA TTAATAATAT AAGGGAAGCT 
AGATATGAAC AGCAATTGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_015461.1
CDS	1..2784
Translation	MVSVIDKLVFDFGGKTLVSLAPDNNTLCVANKNGLTKILKTNNPEEEPETLDSSKLVSSIKCYSNSHFLMTTMQGDALRYNIDSSQEELLARFALPLRDCCVIHSGKMAVFGGDDLELILLELDDETHKKHAIKIDEQVSQISYNSQMNILAVSMINGKVQIFSLTSTIPNKVHELNDYIVANSYDDTHRDKILSNMMDDIDKDNDNDLSETADPDENNVADPEFCAANRICTRVAWHPKGLHFALPCADDTVKIFSIKGYSLQKTLSTNLSSTKAHFIDLQFDPLRGTYIAAVDLNNKLTVWNWETSEIHYTREFKRKITNIAWKIQADSKTLDLVLGTWSGSIAIVQNLAESVVSNIPDQSVAESSTKHGLFVDSESDLENLEGNDDINKSDKLFSDITQEANAEDVFTQTHDGPSGLSEKRKYNFEDEEDFIDDDDGAGYISGKKPHNEHSYSRVHKTHSFPISLANTGKFRYMPFSPAGTPFGFTDRRYLTMNEVGYVSTVKNSEQYSITVSFFDVGRFREYHFEDLFGYDLCFLNEKGTLFGQSKTGQIQYRPHDSIHSNWTKIIPLQAGERITSVAATPVRVIVGTSLGYFRSFNQFGVPFAVEKTSPIVALTAQNYRVFSVHYSQFHGLSYSLSELGTSSKRYYKRECPLPMSLPNINSDMKKDANLDYYNFNPMGIKSLFFSSYGDPCIFGSDNTLLLLSKWRSPEESKWLPILDSNMEIWKMSGGKETTDIHVWPLALAYDTLNCILVKGKHIWPEFPLPLPSEMEIRMPVFVKSKLLEENKAILNKKNEIGADTEAEEGEEDKEIQIPVSMAAEEEYLRSKVLSELLTDTLENDGEMYGNENEVLAALNGAYDKALLRLFASACSDQNVEKALSLAHELKQDRALTAAVKISERAELPSLVKKINNIREARYEQQLK

Target ORF information:

RefSeq Version	NM_001184232.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C chromatin-binding protein CTF4 (CTF4), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184232.1

ORF Insert Sequence:

ATGGTTTCAG TTATAGACAA GCTTGTTTTT GACTTTGGTG GGAAAACTCT GGTCTCTCTC 
GCACCAGATA ATAATACTTT GTGTGTAGCC AATAAAAATG GCTTAACCAA GATTCTGAAG 
ACAAATAACC CAGAAGAAGA GCCAGAGACT TTAGATTCTT CCAAATTGGT CTCGTCTATA 
AAATGCTATT CAAACTCGCA CTTTCTGATG ACTACAATGC AAGGTGATGC TCTTAGGTAT 
AACATCGATT CTAGTCAAGA AGAATTATTG GCTAGATTTG CTTTACCCCT ACGTGACTGC 
TGTGTTATTC ATTCAGGTAA AATGGCCGTA TTTGGAGGAG ACGACTTAGA ACTAATCCTT 
TTGGAATTGG ACGATGAAAC ACACAAAAAA CATGCCATTA AAATCGATGA ACAAGTTTCT 
CAAATTTCTT ACAATTCACA GATGAATATT TTAGCAGTTT CAATGATAAA TGGTAAGGTA 
CAAATTTTTT CTCTGACATC TACTATTCCA AACAAAGTTC ATGAGTTAAA TGATTACATA 
GTGGCCAATT CATATGATGA TACACACAGA GATAAGATAC TCTCGAATAT GATGGATGAT 
ATAGATAAAG ACAATGATAA TGACCTGAGT GAAACGGCTG ATCCAGATGA GAACAATGTA 
GCTGATCCAG AATTCTGTGC TGCTAATAGA ATTTGCACGA GAGTGGCTTG GCATCCGAAG 
GGTCTGCATT TTGCGCTACC ATGTGCAGAT GATACAGTAA AAATATTCTC TATAAAGGGA 
TATTCCCTAC AAAAGACGTT GTCCACAAAT CTCTCATCAA CAAAGGCTCA TTTCATTGAT 
TTGCAATTTG ACCCGTTACG TGGAACTTAC ATTGCGGCAG TAGATTTAAA TAATAAGTTA 
ACGGTATGGA ATTGGGAAAC TTCCGAGATC CACTACACCA GGGAATTCAA AAGAAAAATT 
ACTAATATTG CCTGGAAAAT CCAAGCGGAC TCAAAAACTC TAGACCTTGT TTTAGGTACC 
TGGTCCGGTA GTATAGCCAT TGTCCAAAAT TTGGCAGAGT CCGTAGTATC TAATATACCT 
GACCAATCTG TTGCTGAATC TTCAACTAAA CATGGGCTTT TTGTAGACTC CGAATCTGAC 
TTGGAAAACT TAGAGGGAAA TGATGATATA AACAAAAGCG ATAAGCTATT TTCAGATATT 
ACTCAAGAAG CGAATGCGGA AGATGTGTTC ACTCAAACAC ACGACGGCCC CAGTGGATTA 
AGTGAAAAGA GAAAATACAA CTTCGAAGAT GAAGAAGACT TTATTGATGA TGACGATGGT 
GCTGGTTATA TTAGTGGCAA AAAACCACAT AATGAACATT CTTATTCGAG AGTACACAAG 
ACTCATTCGT TTCCAATCAG TTTGGCAAAC ACAGGAAAAT TTCGTTATAT GCCTTTTTCT 
CCAGCGGGAA CACCTTTTGG CTTTACTGAC AGGCGTTATT TGACAATGAA TGAAGTGGGC 
TACGTATCTA CTGTCAAGAA TAGTGAGCAA TACAGCATAA CTGTCTCTTT TTTTGATGTT 
GGACGTTTTA GAGAATACCA TTTCGAGGAC TTATTTGGCT ACGATTTATG CTTCCTAAAT 
GAAAAAGGCA CTTTATTTGG CCAATCCAAA ACTGGGCAGA TACAATATAG GCCACACGAT 
AGCATACATT CAAACTGGAC CAAGATTATT CCTTTGCAAG CTGGTGAGAG AATAACAAGT 
GTGGCAGCCA CCCCGGTTCG CGTTATTGTT GGTACATCAT TAGGCTATTT CAGAAGTTTC 
AATCAATTCG GGGTTCCATT TGCTGTTGAA AAGACATCCC CAATTGTAGC GCTTACTGCT 
CAGAATTATA GGGTTTTTTC AGTACATTAT TCGCAGTTTC ATGGCCTTTC ATACTCTTTA 
TCTGAATTGG GTACTTCTAG TAAAAGGTAC TATAAAAGAG AGTGTCCACT TCCAATGAGT 
TTACCAAACA TTAATTCTGA TATGAAAAAA GACGCAAATC TTGACTACTA CAATTTTAAT 
CCGATGGGCA TCAAAAGTTT GTTCTTTTCA AGCTACGGAG ATCCATGCAT TTTTGGGTCC 
GACAACACGC TTCTATTGTT ATCAAAGTGG AGATCACCAG AAGAAAGTAA ATGGCTCCCT 
ATTCTAGATA GCAACATGGA AATATGGAAG ATGTCAGGAG GGAAGGAAAC GACAGATATA 
CATGTCTGGC CCTTGGCTTT GGCGTATGAC ACATTGAATT GTATCTTAGT TAAGGGCAAG 
CATATATGGC CCGAGTTTCC CCTTCCGTTG CCATCCGAAA TGGAGATTAG AATGCCAGTA 
TTTGTTAAGA GTAAATTACT AGAGGAAAAC AAAGCTATAT TAAATAAAAA GAACGAAATT 
GGAGCTGACA CCGAAGCGGA AGAAGGGGAA GAAGACAAAG AAATACAGAT TCCTGTTTCT 
ATGGCGGCGG AAGAAGAGTA TCTGCGCAGC AAGGTTTTGT CAGAGCTGTT GACAGATACA 
CTCGAAAATG ACGGTGAAAT GTACGGCAAC GAAAATGAGG TATTGGCAGC ATTGAACGGT 
GCATATGATA AGGCTTTGTT ACGTTTATTT GCGTCTGCAT GCTCAGACCA AAATGTTGAA 
AAGGCTCTTT CGCTTGCTCA TGAATTAAAA CAAGATAGAG CACTTACTGC AGCCGTTAAG 
ATATCAGAAA GAGCTGAGCT GCCCTCTCTC GTTAAAAAAA TTAATAATAT AAGGGAAGCT 
AGATATGAAC AGCAATTGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.
Nature387(6632 Suppl)103-5(1997 May)
Bussey H,Storms RK,Ahmed A,Albermann K,Allen E,Ansorge W,Araujo R,Aparicio A,Barrell B,Badcock K,Benes V,Botstein D,Bowman S,Br?ckner M,Carpenter J,Cherry JM,Chung E,Churcher C,Coster F,Davis K,Davis RW,Dietrich FS,Delius H,DiPaolo T,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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CTF4 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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