MEP2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MEP2
Entrez Gene ID	855580
Full Name	ammonium permease MEP2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182980.1	NP_014257.1	ammonium permease MEP2

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0D07744g	XP_453408.1
Kluyveromyces lactis	KLLA0D07766g	XP_453409.1
Oryza sativa (rice)	Os02g0550800	NP_001047097.1
Magnaporthe oryzae (rice blast fungus)	MGG_16392	XP_003710394.1
Neurospora crassa	NCU01065	XP_961677.1
Saccharomyces cerevisiae (baker's yeast)	MEP2	NP_014257.1
Schizosaccharomyces pombe (fission yeast)	amt1	NP_588424.1
Oryza sativa (rice)	Os01g0870300	NP_001044932.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Philippsen P, Kleine K, P?hlmann R, D?sterh?ft A, Hamberg K, Hegemann JH, Obermaier B, Urrestarazu LA, Aert R, Albermann K, Altmann R, Andr? B, Baladron V, Ballesta JP, B?cam AM, Beinhauer J, Boskovic J, Buitrago MJ, Bussereau F, Coster F, Crouzet M, D'Angelo M, Dal Pero F, De Antoni A, Del Rey F, Doignon F, Domdey H, Dubois E, Fiedler T, Fleig U, Floeth M, Fritz C, Gaillardin C, Garcia-Cantalejo JM, Glansdorff NN, Goffeau A, Gueldener U, Herbert C, Heumann K, Heuss-Neitzel D, Hilbert H, Hinni K, Iraqui Houssaini I, Jacquet M, Jimenez A, Jonniaux JL, Karpfinger L, Lanfranchi G, Lepingle A, Levesque H, Lyck R, Maftahi M, Mallet L, Maurer KC, Messenguy F, Mewes HW, M?sti D, Nasr F, Nicaud JM, Niedenthal RK, Pandolfo D, Pi?rard A, Piravandi E, Planta RJ, Pohl TM, Purnelle B, Rebischung C, Remacha M, Revuelta JL, Rinke M, Saiz JE, Sartorello F, Scherens B, Sen-Gupta M, Soler-Mira A, Urbanus JH, Valle G, Van Dyck L, Verhasselt P, Vierendeels F, Vissers S, Voet M, Volckaert G, Wach A, Wambutt R, Wedler H, Zollner A, Hani J
Nature387(6632 Suppl)93-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

X-ray crystal structures are being reported of the Mep2 orthologues from Saccharomyces cerevisiae and Candida albicans showing that under nitrogen-sufficient conditions the transporters are not phosphorylated and present in closed, inactive conformations.
Title: Structural basis for Mep2 ammonium transceptor activation by phosphorylation.

Mep2-like proteins are nutrient sensing transceptors that govern cellular differentiation
Title: A Mep2-dependent transcriptional profile links permease function to gene expression during pseudohyphal growth in Saccharomyces cerevisiae.

The growth impairment of cells lacking Npr1 on many nitrogen sources is shared by cells deprived of the three Mep proteins and is a consequence of deficient ammonium retrieval.
Title: The yeast ammonium transport protein Mep2 and its positive regulator, the Npr1 kinase, play an important role in normal and pseudohyphal growth on various nitrogen media through retrieval of excreted ammonium.

an ammonium permease MEP2-based sensing mechanism for rapid activation of the protein kinase A pathway
Title: Ammonium permease-based sensing mechanism for rapid ammonium activation of the protein kinase A pathway in yeast.

The following MEP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MEP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi04495	NM_001182980.1 Latest version!	Saccharomyces cerevisiae S288C ammonium permease MEP2 (MEP2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MEP2 ( NM_001182980.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014257.1 Saccharomyces cerevisiae S288C ammonium permease MEP2 (MEP2), partial mRNA.

Price & Availability↑

CloneID	OSi04495
Clone ID Related Accession (Same CDS sequence)	NM_001182980.1
Accession Version	NM_001182980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	ammonium permease MEP2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001146). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTTACA ATTTTACAGG TACGCCTACA GGCGAAGGAA CGGGTGGTAA CTCGTTGACA 
ACAGATTTGA ATACACAATT TGACTTGGCC AACATGGGAT GGATCGGTGT GGCTTCAGCA 
GGTGTGTGGA TTATGGTCCC AGGTATCGGT TTATTATATT CTGGTTTATC CAGGAAAAAG 
CATGCTTTAT CCTTGCTTTG GGCCTCGATG ATGGCTTCCG CCGTGTGTAT TTTCCAATGG 
TTTTTCTGGG GATACTCATT AGCTTTCTCA CACAACACTA GAGGTAACGG TTTTATTGGT 
ACCTTGGAAT TCTTTGGGTT TCGTAACGTT TTAGGAGCCC CATCTAGTGT CAGTTCTCTT 
CCCGATATAC TGTTTGCCGT TTACCAAGGT ATGTTTGCCG CAGTCACCGG TGCCCTAATG 
CTAGGTGGTG CCTGCGAGAG GGCAAGGTTG TTTCCTATGA TGGTGTTCTT GTTTTTATGG 
ATGACTATTG TTTATTGTCC TATTGCATGC TGGGTCTGGA ATGCCGAGGG TTGGTTGGTC 
AAATTGGGTA GCTTGGACTA TGCAGGTGGT TTATGTGTCC ATTTAACATC TGGACATGGT 
GGTCTAGTTT ACGCTTTGAT ACTGGGTAAG CGTAATGACC CTGTTACACG TAAAGGGATG 
CCCAAGTACA AACCACATTC CGTCACCTCG GTGGTTTTAG GCACAGTGTT CTTATGGTTT 
GGTTGGATGT TCTTTAACGG AGGCTCTGCA GGTAATGCAA CTATACGAGC ATGGTACTCT 
ATTATGTCCA CAAACTTAGC TGCTGCTTGC GGTGGCTTGA CATGGATGGT TATCGATTAT 
TTCAGATGCG GAAGAAAGTG GACTACAGTT GGTTTGTGTT CAGGTATCAT CGCTGGCCTA 
GTGGGTATCA CCCCAGCCGC CGGGTTCGTG CCAATCTGGT CAGCCGTTGT CATTGGTGTG 
GTTACTGGTG CAGGATGTAA CCTTGCTGTT GACTTAAAGA GTCTATTGCG CATCGATGAT 
GGTCTAGATT GTTACTCTAT CCATGGTGTG GGTGGTTGTA TTGGTTCTGT ATTAACTGGT 
ATCTTTGCTG CAGACTATGT AAATGCCACT GCAGGCTCTT ACATTAGTCC AATTGATGGT 
GGCTGGATCA ATCATCACTA TAAACAAGTT GGTTATCAAT TAGCAGGTAT ATGCGCTGCA 
CTAGCCTGGA CTGTTACTGT CACATCTATC TTGCTTCTAA CTATGAATGC CATTCCATTT 
TTAAAACTAA GATTAAGTGC TGATGAGGAA GAATTAGGTA CCGACGCTGC TCAAATTGGT 
GAATTTACAT ACGAGGAATC CACTGCTTAC ATCCCAGAAC CAATCAGATC TAAAACATCG 
GCACAAATGC CACCTCCTCA TGAAAACATT GATGATAAGA TTGTGGGTAA CACAGACGCA 
GAAAAGAATT CTACGCCTTC CGACGCTTCT TCTACTAAGA ACACTGACCA TATAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014257.1
CDS	1..1500
Translation	MSYNFTGTPTGEGTGGNSLTTDLNTQFDLANMGWIGVASAGVWIMVPGIGLLYSGLSRKKHALSLLWASMMASAVCIFQWFFWGYSLAFSHNTRGNGFIGTLEFFGFRNVLGAPSSVSSLPDILFAVYQGMFAAVTGALMLGGACERARLFPMMVFLFLWMTIVYCPIACWVWNAEGWLVKLGSLDYAGGLCVHLTSGHGGLVYALILGKRNDPVTRKGMPKYKPHSVTSVVLGTVFLWFGWMFFNGGSAGNATIRAWYSIMSTNLAAACGGLTWMVIDYFRCGRKWTTVGLCSGIIAGLVGITPAAGFVPIWSAVVIGVVTGAGCNLAVDLKSLLRIDDGLDCYSIHGVGGCIGSVLTGIFAADYVNATAGSYISPIDGGWINHHYKQVGYQLAGICAALAWTVTVTSILLLTMNAIPFLKLRLSADEEELGTDAAQIGEFTYEESTAYIPEPIRSKTSAQMPPPHENIDDKIVGNTDAEKNSTPSDASSTKNTDHIV

Target ORF information:

RefSeq Version	NM_001182980.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C ammonium permease MEP2 (MEP2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182980.1

ORF Insert Sequence:

ATGTCTTACA ATTTTACAGG TACGCCTACA GGCGAAGGAA CGGGTGGTAA CTCGTTGACA 
ACAGATTTGA ATACACAATT TGACTTGGCC AACATGGGAT GGATCGGTGT GGCTTCAGCA 
GGTGTGTGGA TTATGGTCCC AGGTATCGGT TTATTATATT CTGGTTTATC CAGGAAAAAG 
CATGCTTTAT CCTTGCTTTG GGCCTCGATG ATGGCTTCCG CCGTGTGTAT TTTCCAATGG 
TTTTTCTGGG GATACTCATT AGCTTTCTCA CACAACACTA GAGGTAACGG TTTTATTGGT 
ACCTTGGAAT TCTTTGGGTT TCGTAACGTT TTAGGAGCCC CATCTAGTGT CAGTTCTCTT 
CCCGATATAC TGTTTGCCGT TTACCAAGGT ATGTTTGCCG CAGTCACCGG TGCCCTAATG 
CTAGGTGGTG CCTGCGAGAG GGCAAGGTTG TTTCCTATGA TGGTGTTCTT GTTTTTATGG 
ATGACTATTG TTTATTGTCC TATTGCATGC TGGGTCTGGA ATGCCGAGGG TTGGTTGGTC 
AAATTGGGTA GCTTGGACTA TGCAGGTGGT TTATGTGTCC ATTTAACATC TGGACATGGT 
GGTCTAGTTT ACGCTTTGAT ACTGGGTAAG CGTAATGACC CTGTTACACG TAAAGGGATG 
CCCAAGTACA AACCACATTC CGTCACCTCG GTGGTTTTAG GCACAGTGTT CTTATGGTTT 
GGTTGGATGT TCTTTAACGG AGGCTCTGCA GGTAATGCAA CTATACGAGC ATGGTACTCT 
ATTATGTCCA CAAACTTAGC TGCTGCTTGC GGTGGCTTGA CATGGATGGT TATCGATTAT 
TTCAGATGCG GAAGAAAGTG GACTACAGTT GGTTTGTGTT CAGGTATCAT CGCTGGCCTA 
GTGGGTATCA CCCCAGCCGC CGGGTTCGTG CCAATCTGGT CAGCCGTTGT CATTGGTGTG 
GTTACTGGTG CAGGATGTAA CCTTGCTGTT GACTTAAAGA GTCTATTGCG CATCGATGAT 
GGTCTAGATT GTTACTCTAT CCATGGTGTG GGTGGTTGTA TTGGTTCTGT ATTAACTGGT 
ATCTTTGCTG CAGACTATGT AAATGCCACT GCAGGCTCTT ACATTAGTCC AATTGATGGT 
GGCTGGATCA ATCATCACTA TAAACAAGTT GGTTATCAAT TAGCAGGTAT ATGCGCTGCA 
CTAGCCTGGA CTGTTACTGT CACATCTATC TTGCTTCTAA CTATGAATGC CATTCCATTT 
TTAAAACTAA GATTAAGTGC TGATGAGGAA GAATTAGGTA CCGACGCTGC TCAAATTGGT 
GAATTTACAT ACGAGGAATC CACTGCTTAC ATCCCAGAAC CAATCAGATC TAAAACATCG 
GCACAAATGC CACCTCCTCA TGAAAACATT GATGATAAGA TTGTGGGTAA CACAGACGCA 
GAAAAGAATT CTACGCCTTC CGACGCTTCT TCTACTAAGA ACACTGACCA TATAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications.
Nature387(6632 Suppl)93-8(1997 May)
Philippsen P,Kleine K,P?hlmann R,D?sterh?ft A,Hamberg K,Hegemann JH,Obermaier B,Urrestarazu LA,Aert R,Albermann K,Altmann R,Andr? B,Baladron V,Ballesta JP,B?cam AM,Beinhauer J,Boskovic J,Buitrago MJ,Bussereau F,Coster F,Crouzet M,D'Angelo M,Dal Pero F,De Antoni A,Del Rey F,Doignon F,Domdey H,Dubois E,Fiedler T,Fleig U,Floeth M,Fritz C,Gaillardin C,Garcia-Cantalejo JM,Glansdorff NN,Goffeau A,Gueldener U,Herbert C,Heumann K,Heuss-Neitzel D,Hilbert H,Hinni K,Iraqui Houssaini I,Jacquet M,Jimenez A,Jonniaux JL,Karpfinger L,Lanfranchi G,Lepingle A,Levesque H,Lyck R,Maftahi M,Mallet L,Maurer KC,Messenguy F,Mewes HW,M?sti D,Nasr F,Nicaud JM,Niedenthal RK,Pandolfo D,Pi?rard A,Piravandi E,Planta RJ,Pohl TM,Purnelle B,Rebischung C,Remacha M,Revuelta JL,Rinke M,Saiz JE,Sartorello F,Scherens B,Sen-Gupta M,Soler-Mira A,Urbanus JH,Valle G,Van Dyck L,Verhasselt P,Vierendeels F,Vissers S,Voet M,Volckaert G,Wach A,Wambutt R,Wedler H,Zollner A,Hani J

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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MEP2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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