STH1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	STH1
Entrez Gene ID	854680
Full Name	RSC chromatin remodeling complex ATPase subunit STH1
Synonyms	NPS1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179474.1	NP_012140.1	RSC chromatin remodeling complex ATPase subunit STH1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Smarca2	NP_035546.2
Rattus norvegicus (Norway rat)	Smarca2	NP_001004446.1
Saccharomyces cerevisiae (baker's yeast)	STH1	NP_012140.1
Schizosaccharomyces pombe (fission yeast)	snf22	NP_588472.2
Gallus gallus (chicken)	SMARCA2	NP_990470.1
Arabidopsis thaliana (thale cress)	AT5G19310	NP_197432.2
Arabidopsis thaliana (thale cress)	ATCHR12	NP_187252.2
Canis lupus familiaris (dog)	SMARCA2	XP_850159.1
Danio rerio (zebrafish)	smarca2	NP_001038240.1
Eremothecium gossypii	AGOS_AER375C	NP_985231.1
Schizosaccharomyces pombe (fission yeast)	snf21	NP_594861.1
Magnaporthe oryzae (rice blast fungus)	MGG_06388	XP_003717185.1
Neurospora crassa	NCU06488	XP_957128.1
Xenopus tropicalis (tropical clawed frog)	smarca2	XP_004910846.1
Homo sapiens (human)	SMARCA2	NP_001276325.1
Bos taurus (cattle)	SMARCA2	NP_001092585.1
Drosophila melanogaster (fruit fly)	brm	NP_730089.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010462	XP_311484.3
Pan troglodytes (chimpanzee)	SMARCA2	XP_003951484.1
Caenorhabditis elegans (roundworm)	swsn-4	NP_502082.1
Kluyveromyces lactis	KLLA0F04521g	XP_455284.1
Oryza sativa (rice)	Os05g0144300	NP_001054624.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Churcher C, Bowman S, Badcock K, Bankier A, Brown D, Chillingworth T, Connor R, Devlin K, Gentles S, Hamlin N, Harris D, Horsnell T, Hunt S, Jagels K, Jones M, Lye G, Moule S, Odell C, Pearson D, Rajandream M, Rice P, Rowley N, Skelton J, Smith V, Barrell B
Nature387(6632 Suppl)84-7(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sliding-to-ejection involves a continuum of DNA translocation efficiency, consistent with higher magnitudes of ATPase and coupling activities (involving ARPs and Sth1 domains), enabling the simultaneous rupture of multiple histone-DNA contacts facilitating ejection.
Title: Regulation of DNA Translocation Efficiency within the Chromatin Remodeler RSC/Sth1 Potentiates Nucleosome Sliding and Ejection.

Results show that depletion of STH1, the ATPase subunit of RSC, causes enhanced sensitivity to the DNA alkylating agent methyl methanesulfonate (MMS).
Title: Chromatin remodelling complex RSC promotes base excision repair in chromatin of Saccharomyces cerevisiae.

results suggest that Nps1p/RSC plays important roles during the spore development process by controlling gene expression for initiating both meiosis and spore morphogenesis, and ensures proper expression timing of late meiotic genes
Title: RSC Nucleosome-remodeling complex plays prominent roles in transcriptional regulation throughout budding yeast gametogenesis.

The following STH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the STH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03716	NM_001179474.1 Latest version!	Saccharomyces cerevisiae S288C RSC chromatin remodeling complex ATPase subunit STH1 (STH1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

STH1 ( NM_001179474.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012140.1 Saccharomyces cerevisiae S288C RSC chromatin remodeling complex ATPase subunit STH1 (STH1), partial mRNA.

Price & Availability↑

CloneID	OSi03716
Clone ID Related Accession (Same CDS sequence)	NM_001179474.1
Accession Version	NM_001179474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	RSC chromatin remodeling complex ATPase subunit STH1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001141). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGCTTCAGG AACAATCTGA GTTGATGAGC ACGGTGATGA ATAATACACC TACAACAGTT 
GCCGCATTGG CTGCGGTTGC TGCAGCCTCT GAAACAAATG GAAAGCTGGG GTCCGAGGAA 
CAACCTGAAA TCACTATTCC AAAGCCGAGG AGTAGTGCAC AGTTGGAGCA GCTACTCTAC 
CGCTATAGGG CTATTCAAAA CCATCCAAAG GAAAACAAGT TGGAGATCAA GGCAATAGAA 
GACACTTTTA GGAATATATC ACGTGATCAG GATATCTACG AAACAAAGTT GGATACCTTG 
AGAAAGAGTA TAGATAAAGG ATTTCAGTAC GATGAGGATT TATTGAATAA ACACTTGGTT 
GCCTTACAGT TACTGGAGAA AGATACAGAT GTCCCTGACT ACTTCTTAGA CTTACCAGAC 
ACCAAGAACG ATAACACTAC GGCGATCGAA GTAGACTATT CTGAAAAGAA ACCAATAAAA 
ATATCAGCAG ATTTCAATGC GAAGGCCAAA AGTTTGGGTT TAGAATCAAA ATTTTCTAAC 
GCTACGAAGA CTGCATTGGG CGATCCAGAT ACCGAAATAA GGATTTCTGC ACGTATATCC 
AACAGAATTA ACGAGCTGGA AAGATTACCT GCCAATCTGG GTACATACTC ACTAGATGAT 
TGTTTAGAGT TTATTACAAA AGATGATTTA TCGTCCAGAA TGGATACTTT CAAAATCAAA 
GCTCTTGTGG AATTGAAAAG TTTAAAGCTA CTAACTAAAC AAAAATCGAT TAGGCAAAAG 
TTAATCAACA ATGTTGCCAG CCAGGCACAC CACAATATTC CATATCTACG TGATTCTCCT 
TTCACAGCAG CTGCACAAAG ATCTGTTCAA ATTAGAAGCA AAGTTATTGT CCCACAAACC 
GTTCGTTTGG CAGAAGAATT AGAAAGGCAA CAACTATTGG AAAAAAGAAA AAAGGAACGT 
AATTTACATT TACAAAAAAT AAACAGCATC ATTGATTTCA TCAAAGAAAG GCAGTCCGAG 
CAATGGTCTC GTCAAGAACG TTGCTTTCAG TTCGGTAGAT TAGGCGCTTC ATTACACAAT 
CAAATGGAGA AAGATGAACA GAAAAGAATA GAAAGAACTG CTAAGCAACG TTTAGCTGCT 
TTGAAGTCTA ACGATGAGGA GGCTTATTTA AAGCTTTTAG ATCAAACTAA GGATACTAGA 
ATTACACAGT TATTGAGGCA GACTAATTCC TTTTTGGATT CTTTGTCGGA GGCTGTTAGA 
GCACAACAAA ACGAAGCAAA AATTCTTCAT GGTGAGGAAG TCCAACCAAT TACAGATGAA 
GAAAGAGAAA AGACTGACTA TTATGAAGTC GCTCATAGGA TCAAAGAAAA AATTGATAAA 
CAACCTTCTA TTCTTGTTGG CGGCACTTTA AAAGAGTATC AATTACGAGG TTTAGAATGG 
ATGGTGTCGT TGTACAATAA TCATTTGAAC GGTATTCTAG CAGATGAGAT GGGTTTAGGT 
AAAACCATCC AATCTATTTC TCTAATAACA TATCTTTATG AAGTTAAGAA AGACATCGGC 
CCTTTCCTAG TCATTGTACC TTTATCCACA ATAACCAATT GGACTTTAGA ATTTGAAAAA 
TGGGCTCCGA GCTTAAACAC TATTATATAC AAAGGTACAC CAAATCAAAG ACACTCTTTA 
CAGCATCAAA TAAGGGTTGG AAATTTTGAC GTCTTATTGA CAACATATGA GTATATTATT 
AAAGATAAAT CTCTTTTATC AAAACATGAT TGGGCTCATA TGATCATTGA CGAAGGTCAT 
AGAATGAAAA ATGCTCAATC GAAGTTATCA TTCACAATTT CTCATTATTA TCGTACAAGA 
AACAGATTGA TTTTAACGGG TACTCCTTTG CAGAACAATC TACCAGAATT ATGGGCACTG 
TTGAACTTTG TTTTGCCTAA AATTTTCAAT TCTGCGAAGA CTTTTGAAGA TTGGTTTAAT 
ACTCCATTTG CCAACACCGG TACCCAAGAG AAACTAGAAT TAACGGAAGA AGAAACTCTG 
TTAATTATTA GAAGATTACA TAAGGTGTTA AGGCCATTTT TATTGCGTCG TTTGAAAAAG 
GAAGTGGAAA AGGATTTGCC TGATAAAGTG GAAAAAGTTA TCAAATGTAA ACTGTCTGGT 
TTGCAACAAC AGTTATATCA GCAAATGCTA AAGCATAACG CGCTATTTGT TGGTGCAGGG 
ACTGAGGGGG CTACAAAGGG TGGTATTAAA GGTTTGAACA ATAAGATTAT GCAATTAAGA 
AAAATCTGCA ACCATCCATT CGTATTTGAT GAAGTGGAAG GTGTTGTAAA CCCCTCAAGA 
GGTAATAGTG ATTTACTTTT CAGAGTAGCG GGCAAGTTTG AATTACTGGA TAGAGTGCTA 
CCGAAATTCA AAGCCTCAGG CCACAGAGTG CTAATGTTTT TCCAAATGAC TCAAGTTATG 
GATATTATGG AAGACTTCCT AAGAATGAAA GATTTGAAGT ATATGAGATT AGATGGTAGC 
ACTAAGACTG AAGAAAGAAC AGAAATGCTG AATGCATTTA ATGCTCCAGA TTCAGATTAT 
TTCTGTTTCC TTTTGTCAAC TAGAGCCGGT GGTTTGGGTT TGAATTTACA AACTGCAGAT 
ACGGTGATCA TCTTTGATAC AGATTGGAAT CCACACCAAG ATTTACAGGC CCAAGATAGA 
GCTCACAGAA TTGGACAAAA GAATGAGGTT AGAATTTTAA GATTAATTAC CACTGATTCC 
GTAGAGGAAG TTATTTTGGA AAGAGCTATG CAGAAATTGG ACATTGACGG CAAGGTTATC 
CAGGCAGGTA AGTTTGATAA CAAATCAACT GCAGAAGAGC AAGAAGCTTT TTTGAGAAGA 
TTGATCGAAA GTGAAACGAA CAGGGATGAT GATGACAAAG CAGAATTAGA TGACGATGAG 
TTAAACGATA CTTTGGCCAG AAGCGCTGAT GAAAAAATAC TATTTGATAA AATTGATAAG 
GAGAGAATGA ACCAAGAAAG AGCAGATGCT AAAGCGCAAG GCCTGAGAGT CCCTCCTCCT 
AGATTGATTC AATTAGACGA GCTACCCAAA GTATTCAGAG AAGACATTGA AGAACATTTC 
AAGAAAGAGG ATTCTGAACC ATTGGGAAGG ATTAGACAAA AGAAAAGAGT TTACTATGAT 
GACGGTCTAA CTGAAGAACA ATTTTTGGAG GCTGTAGAAG ATGATAATAT GTCTTTAGAA 
GATGCCATTA AAAAAAGACG TGAAGCACGT GAAAGAAGGA GATTACGTCA AAATGGTACA 
AAAGAGAATG AAATAGAAAC CCTGGAAAAC ACGCCTGAAG CATCCGAAAC TTCATTGATT 
GAGAACAATA GCTTCACTGC AGCTGTGGAT GAGGAAACTA ATGCTGATAA AGAGACTACA 
GCTTCCAGGT CAAAACGCCG GAGTAGTAGA AAGAAAAGGA CCATTAGCAT TGTTACGGCA 
GAAGACAAAG AGAATACTCA AGAAGAGAGT ACATCTCAAG AAAACGGGGG CGCTAAGGTG 
GAAGAGGAAG TAAAAAGTAG CTCCGTTGAA ATTATAAATG GTTCAGAATC CAAGAAAAAG 
AAACCGAAAC TGACCGTCAA GATCAAACTA AACAAAACAA CTGTGTTGGA GAATAATGAT 
GGTAAGCGAG CAGAAGAAAA GCCAGAGAGT AAATCGCCCG CGAAGAAGAC TGCAGCCAAA 
AAAACTAAAA CTAAATCCAA GTCGCTTGGG ATTTTTCCCA CGGTGGAAAA ATTGGTGGAA 
GAAATGCGCG AACAGTTAGA TGAGGTAGAC TCCCATCCAA GGACGTCCAT TTTTGAAAAG 
CTTCCTTCGA AACGCGACTA TCCGGACTAT TTTAAAGTAA TTGAAAAACC CATGGCCATT 
GATATTATAT TGAAAAACTG CAAGAATGGC ACTTACAAAA CTTTGGAGGA GGTAAGGCAA 
GCCTTACAAA CGATGTTTGA AAATGCTAGA TTCTATAATG AAGAGGGATC CTGGGTTTAC 
GTTGATGCTG ACAAGTTAAA TGAGTTTACT GATGAATGGT TCAAGGAACA CTCTTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012140.1
CDS	1..4080
Translation	MLQEQSELMSTVMNNTPTTVAALAAVAAASETNGKLGSEEQPEITIPKPRSSAQLEQLLYRYRAIQNHPKENKLEIKAIEDTFRNISRDQDIYETKLDTLRKSIDKGFQYDEDLLNKHLVALQLLEKDTDVPDYFLDLPDTKNDNTTAIEVDYSEKKPIKISADFNAKAKSLGLESKFSNATKTALGDPDTEIRISARISNRINELERLPANLGTYSLDDCLEFITKDDLSSRMDTFKIKALVELKSLKLLTKQKSIRQKLINNVASQAHHNIPYLRDSPFTAAAQRSVQIRSKVIVPQTVRLAEELERQQLLEKRKKERNLHLQKINSIIDFIKERQSEQWSRQERCFQFGRLGASLHNQMEKDEQKRIERTAKQRLAALKSNDEEAYLKLLDQTKDTRITQLLRQTNSFLDSLSEAVRAQQNEAKILHGEEVQPITDEEREKTDYYEVAHRIKEKIDKQPSILVGGTLKEYQLRGLEWMVSLYNNHLNGILADEMGLGKTIQSISLITYLYEVKKDIGPFLVIVPLSTITNWTLEFEKWAPSLNTIIYKGTPNQRHSLQHQIRVGNFDVLLTTYEYIIKDKSLLSKHDWAHMIIDEGHRMKNAQSKLSFTISHYYRTRNRLILTGTPLQNNLPELWALLNFVLPKIFNSAKTFEDWFNTPFANTGTQEKLELTEEETLLIIRRLHKVLRPFLLRRLKKEVEKDLPDKVEKVIKCKLSGLQQQLYQQMLKHNALFVGAGTEGATKGGIKGLNNKIMQLRKICNHPFVFDEVEGVVNPSRGNSDLLFRVAGKFELLDRVLPKFKASGHRVLMFFQMTQVMDIMEDFLRMKDLKYMRLDGSTKTEERTEMLNAFNAPDSDYFCFLLSTRAGGLGLNLQTADTVIIFDTDWNPHQDLQAQDRAHRIGQKNEVRILRLITTDSVEEVILERAMQKLDIDGKVIQAGKFDNKSTAEEQEAFLRRLIESETNRDDDDKAELDDDELNDTLARSADEKILFDKIDKERMNQERADAKAQGLRVPPPRLIQLDELPKVFREDIEEHFKKEDSEPLGRIRQKKRVYYDDGLTEEQFLEAVEDDNMSLEDAIKKRREARERRRLRQNGTKENEIETLENTPEASETSLIENNSFTAAVDEETNADKETTASRSKRRSSRKKRTISIVTAEDKENTQEESTSQENGGAKVEEEVKSSSVEIINGSESKKKKPKLTVKIKLNKTTVLENNDGKRAEEKPESKSPAKKTAAKKTKTKSKSLGIFPTVEKLVEEMREQLDEVDSHPRTSIFEKLPSKRDYPDYFKVIEKPMAIDIILKNCKNGTYKTLEEVRQALQTMFENARFYNEEGSWVYVDADKLNEFTDEWFKEHSS

Target ORF information:

RefSeq Version	NM_001179474.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C RSC chromatin remodeling complex ATPase subunit STH1 (STH1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179474.1

ORF Insert Sequence:

ATGCTTCAGG AACAATCTGA GTTGATGAGC ACGGTGATGA ATAATACACC TACAACAGTT 
GCCGCATTGG CTGCGGTTGC TGCAGCCTCT GAAACAAATG GAAAGCTGGG GTCCGAGGAA 
CAACCTGAAA TCACTATTCC AAAGCCGAGG AGTAGTGCAC AGTTGGAGCA GCTACTCTAC 
CGCTATAGGG CTATTCAAAA CCATCCAAAG GAAAACAAGT TGGAGATCAA GGCAATAGAA 
GACACTTTTA GGAATATATC ACGTGATCAG GATATCTACG AAACAAAGTT GGATACCTTG 
AGAAAGAGTA TAGATAAAGG ATTTCAGTAC GATGAGGATT TATTGAATAA ACACTTGGTT 
GCCTTACAGT TACTGGAGAA AGATACAGAT GTCCCTGACT ACTTCTTAGA CTTACCAGAC 
ACCAAGAACG ATAACACTAC GGCGATCGAA GTAGACTATT CTGAAAAGAA ACCAATAAAA 
ATATCAGCAG ATTTCAATGC GAAGGCCAAA AGTTTGGGTT TAGAATCAAA ATTTTCTAAC 
GCTACGAAGA CTGCATTGGG CGATCCAGAT ACCGAAATAA GGATTTCTGC ACGTATATCC 
AACAGAATTA ACGAGCTGGA AAGATTACCT GCCAATCTGG GTACATACTC ACTAGATGAT 
TGTTTAGAGT TTATTACAAA AGATGATTTA TCGTCCAGAA TGGATACTTT CAAAATCAAA 
GCTCTTGTGG AATTGAAAAG TTTAAAGCTA CTAACTAAAC AAAAATCGAT TAGGCAAAAG 
TTAATCAACA ATGTTGCCAG CCAGGCACAC CACAATATTC CATATCTACG TGATTCTCCT 
TTCACAGCAG CTGCACAAAG ATCTGTTCAA ATTAGAAGCA AAGTTATTGT CCCACAAACC 
GTTCGTTTGG CAGAAGAATT AGAAAGGCAA CAACTATTGG AAAAAAGAAA AAAGGAACGT 
AATTTACATT TACAAAAAAT AAACAGCATC ATTGATTTCA TCAAAGAAAG GCAGTCCGAG 
CAATGGTCTC GTCAAGAACG TTGCTTTCAG TTCGGTAGAT TAGGCGCTTC ATTACACAAT 
CAAATGGAGA AAGATGAACA GAAAAGAATA GAAAGAACTG CTAAGCAACG TTTAGCTGCT 
TTGAAGTCTA ACGATGAGGA GGCTTATTTA AAGCTTTTAG ATCAAACTAA GGATACTAGA 
ATTACACAGT TATTGAGGCA GACTAATTCC TTTTTGGATT CTTTGTCGGA GGCTGTTAGA 
GCACAACAAA ACGAAGCAAA AATTCTTCAT GGTGAGGAAG TCCAACCAAT TACAGATGAA 
GAAAGAGAAA AGACTGACTA TTATGAAGTC GCTCATAGGA TCAAAGAAAA AATTGATAAA 
CAACCTTCTA TTCTTGTTGG CGGCACTTTA AAAGAGTATC AATTACGAGG TTTAGAATGG 
ATGGTGTCGT TGTACAATAA TCATTTGAAC GGTATTCTAG CAGATGAGAT GGGTTTAGGT 
AAAACCATCC AATCTATTTC TCTAATAACA TATCTTTATG AAGTTAAGAA AGACATCGGC 
CCTTTCCTAG TCATTGTACC TTTATCCACA ATAACCAATT GGACTTTAGA ATTTGAAAAA 
TGGGCTCCGA GCTTAAACAC TATTATATAC AAAGGTACAC CAAATCAAAG ACACTCTTTA 
CAGCATCAAA TAAGGGTTGG AAATTTTGAC GTCTTATTGA CAACATATGA GTATATTATT 
AAAGATAAAT CTCTTTTATC AAAACATGAT TGGGCTCATA TGATCATTGA CGAAGGTCAT 
AGAATGAAAA ATGCTCAATC GAAGTTATCA TTCACAATTT CTCATTATTA TCGTACAAGA 
AACAGATTGA TTTTAACGGG TACTCCTTTG CAGAACAATC TACCAGAATT ATGGGCACTG 
TTGAACTTTG TTTTGCCTAA AATTTTCAAT TCTGCGAAGA CTTTTGAAGA TTGGTTTAAT 
ACTCCATTTG CCAACACCGG TACCCAAGAG AAACTAGAAT TAACGGAAGA AGAAACTCTG 
TTAATTATTA GAAGATTACA TAAGGTGTTA AGGCCATTTT TATTGCGTCG TTTGAAAAAG 
GAAGTGGAAA AGGATTTGCC TGATAAAGTG GAAAAAGTTA TCAAATGTAA ACTGTCTGGT 
TTGCAACAAC AGTTATATCA GCAAATGCTA AAGCATAACG CGCTATTTGT TGGTGCAGGG 
ACTGAGGGGG CTACAAAGGG TGGTATTAAA GGTTTGAACA ATAAGATTAT GCAATTAAGA 
AAAATCTGCA ACCATCCATT CGTATTTGAT GAAGTGGAAG GTGTTGTAAA CCCCTCAAGA 
GGTAATAGTG ATTTACTTTT CAGAGTAGCG GGCAAGTTTG AATTACTGGA TAGAGTGCTA 
CCGAAATTCA AAGCCTCAGG CCACAGAGTG CTAATGTTTT TCCAAATGAC TCAAGTTATG 
GATATTATGG AAGACTTCCT AAGAATGAAA GATTTGAAGT ATATGAGATT AGATGGTAGC 
ACTAAGACTG AAGAAAGAAC AGAAATGCTG AATGCATTTA ATGCTCCAGA TTCAGATTAT 
TTCTGTTTCC TTTTGTCAAC TAGAGCCGGT GGTTTGGGTT TGAATTTACA AACTGCAGAT 
ACGGTGATCA TCTTTGATAC AGATTGGAAT CCACACCAAG ATTTACAGGC CCAAGATAGA 
GCTCACAGAA TTGGACAAAA GAATGAGGTT AGAATTTTAA GATTAATTAC CACTGATTCC 
GTAGAGGAAG TTATTTTGGA AAGAGCTATG CAGAAATTGG ACATTGACGG CAAGGTTATC 
CAGGCAGGTA AGTTTGATAA CAAATCAACT GCAGAAGAGC AAGAAGCTTT TTTGAGAAGA 
TTGATCGAAA GTGAAACGAA CAGGGATGAT GATGACAAAG CAGAATTAGA TGACGATGAG 
TTAAACGATA CTTTGGCCAG AAGCGCTGAT GAAAAAATAC TATTTGATAA AATTGATAAG 
GAGAGAATGA ACCAAGAAAG AGCAGATGCT AAAGCGCAAG GCCTGAGAGT CCCTCCTCCT 
AGATTGATTC AATTAGACGA GCTACCCAAA GTATTCAGAG AAGACATTGA AGAACATTTC 
AAGAAAGAGG ATTCTGAACC ATTGGGAAGG ATTAGACAAA AGAAAAGAGT TTACTATGAT 
GACGGTCTAA CTGAAGAACA ATTTTTGGAG GCTGTAGAAG ATGATAATAT GTCTTTAGAA 
GATGCCATTA AAAAAAGACG TGAAGCACGT GAAAGAAGGA GATTACGTCA AAATGGTACA 
AAAGAGAATG AAATAGAAAC CCTGGAAAAC ACGCCTGAAG CATCCGAAAC TTCATTGATT 
GAGAACAATA GCTTCACTGC AGCTGTGGAT GAGGAAACTA ATGCTGATAA AGAGACTACA 
GCTTCCAGGT CAAAACGCCG GAGTAGTAGA AAGAAAAGGA CCATTAGCAT TGTTACGGCA 
GAAGACAAAG AGAATACTCA AGAAGAGAGT ACATCTCAAG AAAACGGGGG CGCTAAGGTG 
GAAGAGGAAG TAAAAAGTAG CTCCGTTGAA ATTATAAATG GTTCAGAATC CAAGAAAAAG 
AAACCGAAAC TGACCGTCAA GATCAAACTA AACAAAACAA CTGTGTTGGA GAATAATGAT 
GGTAAGCGAG CAGAAGAAAA GCCAGAGAGT AAATCGCCCG CGAAGAAGAC TGCAGCCAAA 
AAAACTAAAA CTAAATCCAA GTCGCTTGGG ATTTTTCCCA CGGTGGAAAA ATTGGTGGAA 
GAAATGCGCG AACAGTTAGA TGAGGTAGAC TCCCATCCAA GGACGTCCAT TTTTGAAAAG 
CTTCCTTCGA AACGCGACTA TCCGGACTAT TTTAAAGTAA TTGAAAAACC CATGGCCATT 
GATATTATAT TGAAAAACTG CAAGAATGGC ACTTACAAAA CTTTGGAGGA GGTAAGGCAA 
GCCTTACAAA CGATGTTTGA AAATGCTAGA TTCTATAATG AAGAGGGATC CTGGGTTTAC 
GTTGATGCTG ACAAGTTAAA TGAGTTTACT GATGAATGGT TCAAGGAACA CTCTTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.
Nature387(6632 Suppl)84-7(1997 May)
Churcher C,Bowman S,Badcock K,Bankier A,Brown D,Chillingworth T,Connor R,Devlin K,Gentles S,Hamlin N,Harris D,Horsnell T,Hunt S,Jagels K,Jones M,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Rowley N,Skelton J,Smith V,Barrell B

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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STH1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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