ORF » Species Summary » Saccharomyces cerevisiae S288C » DAS1 cDNA ORF clone

DAS1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following DAS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi02568 NM_001181582.1
Latest version!		 Saccharomyces cerevisiae S288C SCF ubiquitin ligase complex subunit DAS1 (DAS1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    DAS1 ( NM_001181582.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012386.1 Saccharomyces cerevisiae S288C SCF ubiquitin ligase complex subunit DAS1 (DAS1), partial mRNA.
    Price & Availability↑
    CloneID OSi02568
    Clone ID Related Accession (Same CDS sequence) NM_001181582.1
    Accession Version NM_001181582.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product SCF ubiquitin ligase complex subunit DAS1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCCATTTC AAGATTATTT TCAGAAGAAA AAAGCAGCGT TCATTAACAG AAACAATAAA 
    AGTAACGCAG ATGCTTCAGC GCTACGAGAT ATTAACGATA TTAACATAAA TTTCGCCGCC 
    AAGAGCAAAA ACTACGTGTT CCCGCTAACA AAGCTCCCCG ATGAGCTCAT GCAAGAAGTA 
    TTCTCTCATT TGCCGCAACC AGACCGCTTG CAGCTTTGCC TTGTGAACAA AAGGTTGAAT 
    AAGATCGCTA CCAAACTGCT TTATAGAAGA ATATATTTGA ACGACTCAAA TGTAGTGAAG 
    AGTGACTTCA TGCATCTCGC AATTAATTGG ACCCTACTGA ACTTGCCGTC TTCGTTGAAA 
    GAAGAGGAGT CCCGCGATAT CGCCAACTGC AAATTGAAAA AACTTATAGA AACACTGCAA 
    AATAATATTC ACATTACTGA AGTCATCCAG TGGATAAGGA TCAACTGGGA TTTGGACTCT 
    ACCTTGCAAA GGTCCATTCT CAGCATCCTT TGCAATCAGG GCAAGTCCTT GCAAAGACTA 
    GAAAACGTCA CTGACCCGGC TTGTAACGAT ATCATCTCCA ATGGTCACTT TTCCAGGAGC 
    AACGTCTCAA GTTTCGACAT GGCTCCTCCA AATTCATTGC CTGAGATGGT TGTTCCCGAA 
    AATTACATTC CGAATCTCAC CAAATATCTT TCACAGCGTA TCTCTTCTCG GCTTTCTCAT 
    ATGACTTTAT TCATCGACCC TTTAAAACTG TTCAATTATC TTTACCCTTT AGATATCAAG 
    TTACAAATCA TCGATTTAAA ATTGCATTGG AGGAGGGAGT TTTACAATAA TGATTATTTT 
    GTTAAAAAGA TACGACCCGG CAATCCTCTG ACTAAACTAT CGGAGGTTTT TGACAAGAGG 
    ACATTGAAAA TTCTTACCAT CATTTCTTGG AATGACACTT TGTTAAAACG CGAGACAGAA 
    ATGCTGAAAG ATTTTAAAGA ATTTGAGAAC CTGGAAGATT TGTCTTTGAT TTCTATTAAG 
    CAGGATGTTC ATATCCTCGT AGATCTTTTT AGTTCGTTAA CCAATTTGAA AAGACTAAAA 
    ATGGATTTCT TAGAGGAGTA TGTACCTGAA CCCACAAATC CTCACATATT TCTCTCCATT 
    CTTTTAGCTT GCTCCAAATT GCAATTCATA GATCTGAGAT ACGACGGTCT TATCCCACAA 
    ATCATCAATA TTCAAGAAAA TAAGTTCCAA CTAAATCAGC AATGCAACTG CACAAACTGT 
    CAAATAGTAT TCAGTGACAT TCTGAAGGGC AAAATATTCA TGTTCCCTGA AGACTACTAT 
    ATTCACGATC TTCAAGACAT TGCTGCAAAG GATATTTTTA AAATGATGAA ATACCTATCT 
    TTGTTACCAT ACTCTAAAGC TTGTGATGCT TATCCGAGCG TTAGAACACA ACCAATGAAT 
    TTGACTAATT TTGTGACCAA GATGAACCGC AACCTTTTGG AATATAGAAA TTCTAAATCC 
    CAGTTGGTGC CCAAAATCGT TAATAACCCA CATCAACACT CCACCGTGAC TTCCACATCA 
    ACCGCTCATA TGAGCGAGCC CGAAATGATA ATAATCGATG ACGATGATGA TGATGATGAA 
    ATTAATGCGG CCATTCCTCC AAGTAGTGAT GACACAGCAG CTACAATTTC CACTGACCTG 
    GAACTTCCTC ATGAATCATT GACCAAAAGA GATATTATTA TGTGTTATCA CGCTCTTATC 
    CATCACTTCA AGTCGATTTA CGTTACTTTT TTGAAAAGTT TTCCTCATTT GAGATTCTTG 
    ATGTTGAATG ACATTCCCAC AATAGTTATG GAGGAGAATA ATGAACGTAT TTTTGAACCC 
    GTCTTTTATC ATTATGATTA TAAGAGTAAT CTGTATGGAT GGTCAAAGGA ATCGAACAAG 
    AATCTGGAGA ACGACAGCAA CAACAACAAT AATAATTCTG ATACCATTGC AAGAATAGCC 
    ACTGTTATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_012386.1
    CDS1..1992
    Translation

    Target ORF information:

    RefSeq Version NM_001181582.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C SCF ubiquitin ligase complex subunit DAS1 (DAS1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001181582.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCCATTTC AAGATTATTT TCAGAAGAAA AAAGCAGCGT TCATTAACAG AAACAATAAA 
    AGTAACGCAG ATGCTTCAGC GCTACGAGAT ATTAACGATA TTAACATAAA TTTCGCCGCC 
    AAGAGCAAAA ACTACGTGTT CCCGCTAACA AAGCTCCCCG ATGAGCTCAT GCAAGAAGTA 
    TTCTCTCATT TGCCGCAACC AGACCGCTTG CAGCTTTGCC TTGTGAACAA AAGGTTGAAT 
    AAGATCGCTA CCAAACTGCT TTATAGAAGA ATATATTTGA ACGACTCAAA TGTAGTGAAG 
    AGTGACTTCA TGCATCTCGC AATTAATTGG ACCCTACTGA ACTTGCCGTC TTCGTTGAAA 
    GAAGAGGAGT CCCGCGATAT CGCCAACTGC AAATTGAAAA AACTTATAGA AACACTGCAA 
    AATAATATTC ACATTACTGA AGTCATCCAG TGGATAAGGA TCAACTGGGA TTTGGACTCT 
    ACCTTGCAAA GGTCCATTCT CAGCATCCTT TGCAATCAGG GCAAGTCCTT GCAAAGACTA 
    GAAAACGTCA CTGACCCGGC TTGTAACGAT ATCATCTCCA ATGGTCACTT TTCCAGGAGC 
    AACGTCTCAA GTTTCGACAT GGCTCCTCCA AATTCATTGC CTGAGATGGT TGTTCCCGAA 
    AATTACATTC CGAATCTCAC CAAATATCTT TCACAGCGTA TCTCTTCTCG GCTTTCTCAT 
    ATGACTTTAT TCATCGACCC TTTAAAACTG TTCAATTATC TTTACCCTTT AGATATCAAG 
    TTACAAATCA TCGATTTAAA ATTGCATTGG AGGAGGGAGT TTTACAATAA TGATTATTTT 
    GTTAAAAAGA TACGACCCGG CAATCCTCTG ACTAAACTAT CGGAGGTTTT TGACAAGAGG 
    ACATTGAAAA TTCTTACCAT CATTTCTTGG AATGACACTT TGTTAAAACG CGAGACAGAA 
    ATGCTGAAAG ATTTTAAAGA ATTTGAGAAC CTGGAAGATT TGTCTTTGAT TTCTATTAAG 
    CAGGATGTTC ATATCCTCGT AGATCTTTTT AGTTCGTTAA CCAATTTGAA AAGACTAAAA 
    ATGGATTTCT TAGAGGAGTA TGTACCTGAA CCCACAAATC CTCACATATT TCTCTCCATT 
    CTTTTAGCTT GCTCCAAATT GCAATTCATA GATCTGAGAT ACGACGGTCT TATCCCACAA 
    ATCATCAATA TTCAAGAAAA TAAGTTCCAA CTAAATCAGC AATGCAACTG CACAAACTGT 
    CAAATAGTAT TCAGTGACAT TCTGAAGGGC AAAATATTCA TGTTCCCTGA AGACTACTAT 
    ATTCACGATC TTCAAGACAT TGCTGCAAAG GATATTTTTA AAATGATGAA ATACCTATCT 
    TTGTTACCAT ACTCTAAAGC TTGTGATGCT TATCCGAGCG TTAGAACACA ACCAATGAAT 
    TTGACTAATT TTGTGACCAA GATGAACCGC AACCTTTTGG AATATAGAAA TTCTAAATCC 
    CAGTTGGTGC CCAAAATCGT TAATAACCCA CATCAACACT CCACCGTGAC TTCCACATCA 
    ACCGCTCATA TGAGCGAGCC CGAAATGATA ATAATCGATG ACGATGATGA TGATGATGAA 
    ATTAATGCGG CCATTCCTCC AAGTAGTGAT GACACAGCAG CTACAATTTC CACTGACCTG 
    GAACTTCCTC ATGAATCATT GACCAAAAGA GATATTATTA TGTGTTATCA CGCTCTTATC 
    CATCACTTCA AGTCGATTTA CGTTACTTTT TTGAAAAGTT TTCCTCATTT GAGATTCTTG 
    ATGTTGAATG ACATTCCCAC AATAGTTATG GAGGAGAATA ATGAACGTAT TTTTGAACCC 
    GTCTTTTATC ATTATGATTA TAAGAGTAAT CTGTATGGAT GGTCAAAGGA ATCGAACAAG 
    AATCTGGAGA ACGACAGCAA CAACAACAAT AATAATTCTG ATACCATTGC AAGAATAGCC 
    ACTGTTATGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


    Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
    The EMBO journal15(9)2031-49(1996 May)
    Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L


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