ORF » Species Summary » Saccharomyces cerevisiae S288C » EFM5 cDNA ORF clone

EFM5 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following EFM5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EFM5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi02234 NM_001181130.3
Latest version!		 Saccharomyces cerevisiae S288C protein-lysine N-methyltransferase (EFM5), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    EFM5 ( NM_001181130.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_011515.4 Saccharomyces cerevisiae S288C protein-lysine N-methyltransferase (EFM5), partial mRNA.
    Price & Availability↑
    CloneID OSi02234
    Clone ID Related Accession (Same CDS sequence) NM_001181130.3
    Accession Version NM_001181130.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 747bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product protein-lysine N-methyltransferase
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001139). On Jul 30, 2012 this sequence version replaced NM_001181130.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTCCGACT CCGACTCCGA CTCCGATTAT GAATTGACAC TTTCTGCTAA TGCCCTCGCT 
    GCCCTTGAAG AATTCAAAAG AGAGGAACAA CAACATCAAG AAGCCTTTCA AAAGCTTTAC 
    GACGAAACGG ATGAAGACTT CCAAAAGAAA AAAAAAGAAG AAGGGATGAA GCTTTTCAAG 
    GAAGATTGGC AGCTTTCCCA GTTTTGGTAC AGCGATGACA CAGCCGCAAT TTTAGCAGAT 
    GCCATATTGG AAGGTGCGGA CGAAAACACT GTAATTGCAA TAGTTAGTGC GCCATCCGTC 
    TATGCTGCCA TTCAAAAGAA ACCTACTAAT GAAATTCCAA CCGAACATAT CTACTTGTTC 
    GAATTTGATA AGAGATTCGA GTTGTTAGCT GGAAGAGATC ATTTCTTTTT TTATGATTAC 
    AATAAACCAC TGGACTTCAG TGATGAAATT AAAGGAAAAG TTGATAGATT GTTAATTGAC 
    CCACCTTTTT TAAATGAAGA TTGTCAAACA AAGTCCTCTA TCACTGCAAA GTGTTTATTA 
    GCGCCAAATG ACAACTCTAA AACTAAGAAA GGAGTTTTTA AGCACCGCCT CATAAGTTGT 
    ACTGGTGAAA GAATGTCTGA AGTCATATCC AAAGTTTATT CTGACACAAG AATAACAACT 
    TTTCTTCCTG AACATTCTAA TGGTTTGAGT AACGAATTTA GGTGCTACGC AAATTTTGAA 
    TGTAGTTCAT GGAAATTTGC TTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_011515.4
    CDS1..747
    Translation

    Target ORF information:

    RefSeq Version NM_001181130.3
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C protein-lysine N-methyltransferase (EFM5), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001181130.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGTCCGACT CCGACTCCGA CTCCGATTAT GAATTGACAC TTTCTGCTAA TGCCCTCGCT 
    GCCCTTGAAG AATTCAAAAG AGAGGAACAA CAACATCAAG AAGCCTTTCA AAAGCTTTAC 
    GACGAAACGG ATGAAGACTT CCAAAAGAAA AAAAAAGAAG AAGGGATGAA GCTTTTCAAG 
    GAAGATTGGC AGCTTTCCCA GTTTTGGTAC AGCGATGACA CAGCCGCAAT TTTAGCAGAT 
    GCCATATTGG AAGGTGCGGA CGAAAACACT GTAATTGCAA TAGTTAGTGC GCCATCCGTC 
    TATGCTGCCA TTCAAAAGAA ACCTACTAAT GAAATTCCAA CCGAACATAT CTACTTGTTC 
    GAATTTGATA AGAGATTCGA GTTGTTAGCT GGAAGAGATC ATTTCTTTTT TTATGATTAC 
    AATAAACCAC TGGACTTCAG TGATGAAATT AAAGGAAAAG TTGATAGATT GTTAATTGAC 
    CCACCTTTTT TAAATGAAGA TTGTCAAACA AAGTCCTCTA TCACTGCAAA GTGTTTATTA 
    GCGCCAAATG ACAACTCTAA AACTAAGAAA GGAGTTTTTA AGCACCGCCT CATAAGTTGT 
    ACTGGTGAAA GAATGTCTGA AGTCATATCC AAAGTTTATT CTGACACAAG AATAACAACT 
    TTTCTTCCTG AACATTCTAA TGGTTTGAGT AACGAATTTA GGTGCTACGC AAATTTTGAA 
    TGTAGTTCAT GGAAATTTGC TTCTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.
    Nature387(6632 Suppl)81-4(1997 May)
    Tettelin H,Agostoni Carbone ML,Albermann K,Albers M,Arroyo J,Backes U,Barreiros T,Bertani I,Bjourson AJ,Br?ckner M,Bruschi CV,Carignani G,Castagnoli L,Cerdan E,Clemente ML,Coblenz A,Coglievina M,Coissac E,Defoor E,Del Bino S,Delius H,Delneri D,de Wergifosse P,Dujon B,Kleine K


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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