MEC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	MEC1
Entrez Gene ID	852433
Full Name	protein kinase MEC1
Synonyms	ESR1,RAD31,SAD3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178484.3	NP_009694.3	protein kinase MEC1

Pathways from BioSystems

Homologies

Saccharomyces cerevisiae (baker's yeast)	MEC1	NP_009694.3
Kluyveromyces lactis	KLLA0C15785g	XP_452905.1
Eremothecium gossypii	AGOS_ABR108C	NP_983055.2
Schizosaccharomyces pombe (fission yeast)	rad3	NP_595357.1
Magnaporthe oryzae (rice blast fungus)	MGG_02656	XP_003721126.1
Neurospora crassa	NCU11188	XP_001728458.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete DNA sequence of yeast chromosome II.
Feldmann H, Aigle M, Aljinovic G, Andr? B, Baclet MC, Barthe C, Baur A, B?cam AM, Biteau N, Boles E, Brandt T, Brendel M, Br?ckner M, Bussereau F, Christiansen C, Contreras R, Crouzet M, Cziepluch C, D?molis N, Delaveau T, Doignon F, Domdey H, D?sterhus S, Dubois E, Dujon B, El Bakkoury M, Entian KD, Feurmann M, Fiers W, Fobo GM, Fritz C, Gassenhuber H, Glandsdorff N, Goffeau A, Grivell LA, de Haan M, Hein C, Herbert CJ, Hollenberg CP, Holmstr?m K, Jacq C, Jacquet M, Jauniaux JC, Jonniaux JL, Kalles?e T, Kiesau P, Kirchrath L, K?tter P, Korol S, Liebl S, Logghe M, Lohan AJ, Louis EJ, Li ZY, Maat MJ, Mallet L, Mannhaupt G, Messenguy F, Miosga T, Molemans F, M?ller S, Nasr F, Obermaier B, Perea J, Pi?rard A, Piravandi E, Pohl FM, Pohl TM, Potier S, Proft M, Purnelle B, Ramezani Rad M, Rieger M, Rose M, Schaaff-Gerstenschl?ger I, Scherens B, Schwarzlose C, Skala J, Slonimski PP, Smits PH, Souciet JL, Steensma HY, Stucka R, Urrestarazu A, van der Aart QJ, van Dyck L, Vassarotti A, Vetter I, Vierendeels F, Vissers S, Wagner G, de Wergifosse P, Wolfe KH, Zagulski M, Zimmermann FK, Mewes HW, Kleine K
The EMBO journal13(24)5795-809(1994 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RPA-dependent recruitment of Mec1-Ddc2 is crucial for maintaining its homodimeric state at ssDNA and that Ddc2's recruitment domain and CCD are important for Mec1-dependent survival of UV-light-induced DNA damage.
Title: Structural Basis of Mec1-Ddc2-RPA Assembly and Activation on Single-Stranded DNA at Sites of Damage.

Our findings provide a model in which two different Tel2 pathways promote protein stabilization of Mec1 and Tel1 in budding yeast.
Title: Two separate pathways regulate protein stability of ATM/ATR-related protein kinases Mec1 and Tel1 in budding yeast.

hese unusual mcm2DENQ phenotypes are markedly similar to those of a special previously-studied allele of the checkpoint sensor kinase ATR/MEC1, suggesting a possible regulatory interplay between Mcm2-7 and ATR during unchallenged growth.
Title: A Checkpoint-Related Function of the MCM Replicative Helicase Is Required to Avert Accumulation of RNA:DNA Hybrids during S-phase and Ensuing DSBs during G2/M.

Mec1 is highly activated during normal DNA replication.
Title: Phosphoproteomics reveals distinct modes of Mec1/ATR signaling during DNA replication.

the SWI/SNF chromatin remodeling complex is capable of regulating Mec1 kinase activity
Title: Regulation of Mec1 kinase activity by the SWI/SNF chromatin remodeling complex.

The following MEC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MEC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01839	NM_001178484.3 Latest version!	Saccharomyces cerevisiae S288C protein kinase MEC1 (MEC1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MEC1 ( NM_001178484.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_009694.3 Saccharomyces cerevisiae S288C protein kinase MEC1 (MEC1), partial mRNA.

Price & Availability↑

CloneID	OSi01839
Clone ID Related Accession (Same CDS sequence)	NM_001178484.3
Accession Version	NM_001178484.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	protein kinase MEC1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001134). On Jul 30, 2012 this sequence version replaced NM_001178484.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGAATCAC ACGTCAAATA TCTTGACGAA TTGATATTGG CAATAAAAGA CCTGAACTCG 
GGGGTGGATT CAAAGGTGCA GATTAAAAAA GTGCCCACGG ATCCATCTTC TTCTCAGGAG 
TACGCCAAGA GTTTAAAGAT CCTGAACACC CTCATAAGAA ACCTAAAAGA TCAAAGAAGG 
AACAATATCA TGAAAAATGA TACTATATTT TCGAAAACAG TTTCCGCCCT TGCCTTATTG 
TTGGAGTACA ACCCCTTCTT GCTTGTTATG AAGGATTCCA ACGGGAACTT TGAGATACAA 
AGGCTGATAG ATGATTTCCT CAACATATCC GTTCTGAACT ATGATAATTA CCACAGAATA 
TGGTTTATGA GGCGAAAATT AGGCAGCTGG TGCAAAGCAT GTGTCGAATT TTACGGAAAA 
CCTGCTAAGT TTCAGCTTAC TGCACATTTT GAGAACACCA TGAATCTTTA CGAACAGGCC 
TTGACTGAAG TCTTGTTGGG CAAGACTGAG CTTCTCAAAT TTTATGACAC CTTGAAGGGT 
CTATACATTC TTTTATACTG GTTCACTTCG GAGTATAGTA CTTTTGGGAA CTCTATAGCA 
TTCTTAGATT CTTCTTTGGG GTTCACGAAA TTTGACTTTA ACTTCCAACG ATTAATCAGG 
ATTGTTCTTT ACGTCTTTGA TTCCTGCGAA CTAGCAGCAC TAGAATATGC CGAAATCCAA 
CTCAAATATA TTTCTCTAGT TGTGGACTAT GTTTGCAATA GAACAATTTC CACAGCCCTG 
GATGCCCCAG CGTTAGTTTG TTGTGAACAA TTAAAGTTTG TATTGACTAC TATGCATCAT 
TTTTTGGATA ACAAGTATGG GCTCTTGGAT AATGACCCCA CTATGGCCAA AGGAATTCTT 
CGACTATATT CTCTTTGCAT TTCTAACGAT TTCTCAAAAT GCTTTGTAGA CCACTTCCCA 
ATTGACCAGT GGGCAGATTT TTCACAAAGT GAACATTTTC CGTTCACGCA GTTGACTAAT 
AAAGCTCTCT CGATTGTATA TTTTGATTTG AAAAGAAGGT CCCTACCTGT TGAAGCTTTA 
AAGTACGATA ATAAGTTCAA CATCTGGGTA TACCAATCGG AGCCGGACTC GAGCTTGAAA 
AATGTCACTT CTCCCTTTGA TGATCGATAT AAGCAGCTGG AAAAGCTAAG GTTGCTAGTA 
CTAAAGAAGT TTAACAAGAC AGAAAGAGGA ACTTTGCTCA AATACCGCGT GAACCAGCTA 
AGTCCTGGAT TTTTTCAAAG AGCTGGAAAC GATTTCAAGC TAATTTTAAA TGAAGCATCT 
GTATCCATTC AAACTTGTTT CAAGACAAAC AATATAACAA GGCTAACATC ATGGACTGTA 
ATTCTCGGAC GTCTAGCCTG TCTAGAATCA GAGAAGTTTT CCGGCACTCT GCCAAATTCC 
ACAAAGGATA TGGATAATTG GTATGTTTGT CATTTATGCG ATATTGAGAA AACTGGCAAC 
CCTTTCGTGC GAATAAATCC AAATAGACCA GAGGCTGCGG GTAAATCAGA AATCTTCAGG 
ATACTTCATT CAAACTTTCT ATCTCACCCA AATATAGATG AATTTAGCGA ATCTTTGTTA 
AGTGGCATCT TATTTTCTCT ACATAGGATA TTTTCACACT TTCAACCTCC AAAACTTACA 
GATGGAAACG GTCAAATCAA TAAGAGCTTT AAACTGGTAC AAAAGTGCTT TATGAATTCT 
AACAGATACC TACGTTTATT AAGTACTAGA ATTATACCTT TATTCAATAT ATCAGACTCT 
CATAATTCCG AAGATGAACA CACTGCCACG CTGATAAAGT TTCTACAATC TCAAAAATTG 
CCAGTGGTGA AAGAAAATTT AGTCATTGCT TGGACACAAT TAACATTGAC GACTTCTAAT 
GATGTATTTG ATACACTACT TTTGAAACTG ATTGATATTT TCAATTCTGA TGATTATAGT 
TTACGAATAA TGATGACTTT GCAAATTAAA AATATGGCCA AAATTTTAAA GAAAACACCA 
TATCAATTAC TATCGCCTAT TTTACCTGTA TTACTAAGAC AGTTGGGAAA AAACCTCGTG 
GAAAGAAAAG TTGGCTTTCA AAATTTAATA GAATTATTGG GATATTCTTC AAAAACAATT 
CTCGATATTT TCCAGAGATA TATCATCCCT TATGCAATTA TTCAATATAA GAGCGATGTG 
CTAAGTGAAA TTGCTAAGAT TATGTGTGAT GGCGATACAA GTTTAATTAA CCAAATGAAG 
GTTAATTTAC TGAAAAAAAA CAGTAGGCAA ATATTTGCCG TAGCTTTGGT AAAACACGGA 
TTATTTTCTT TGGATATCTT GGAAACCCTT TTTTTAAATA GGGCTCCAAC TTTTGACAAA 
GGATATATAA CTGCATACCT TCCCGATTAT AAAACTTTAG CTGAAATAAC GAAGCTCTAC 
AAAAACAGCG TTACTAAAGA TGCAAGTGAC AGCGAGAATG CTAATATGAT TTTATGCTCT 
TTGCGATTTT TAATCACCAA TTTTGAAAAA GACAAAAGGC ATGGTTCGAA GTACAAAAAT 
ATCAATAACT GGACGGATGA TCAGGAACAA GCGTTCCAAA AGAAACTACA GGATAATATC 
TTAGGTATTT TCCAAGTTTT TTCGAGTGAC ATACATGATG TTGAAGGCCG CACCACTTAC 
TACGAAAAGT TAAGGGTTAT CAATGGCATT TCTTTTCTTA TCATATATGC CCCCAAAAAA 
TCAATAATTT CCGCATTAGC CCAGATTAGT ATTTGTTTGC AAACAGGACT TGGGCTTAAG 
GAAGTTCGAT ACGAGGCCTT TAGATGTTGG CATCTGTTAG TTCGCCATCT AAATGATGAA 
GAACTCTCTA CTGTTATAGA TAGCTTAATT GCATTCATAC TTCAAAAGTG GTCTGAGTTC 
AACGGAAAAC TTCGAAATAT AGTGTACAGT ATACTGGATA CCTTAATCAA AGAGAAATCG 
GACCTGATTT TGAAATTAAA ACCTTACACT ACTTTGGCTT TAGTAGGCAA GCCTGAATTA 
GGTATTTTAG CTCGTGATGG CCAATTTGCA AGAATGGTGA ATAAAATAAG AAGTACCACG 
GACCTTATAC CCATATTTGC TAATAACTTG AAAAGTAGTA ACAAGTATGT CATAAACCAA 
AATTTAGACG ATATAGAGGT ATATCTTCGG AGAAAGCAGA CAGAAAGATC GATTGATTTT 
ACACCAAAGA AGGTTGGGCA AACTTCTGAT ATAACATTAG TTTTGGGTGC TTTATTAGAC 
ACTTCTCATA AGTTTAGAAA TTTAGACAAG GACCTATGCG AGAAGTGCGC CAAATGTATC 
AGTATGATTG GTGTTTTAGA CGTTACAAAG CATGAGTTTA AAAGAACAAC ATATTCAGAA 
AACGAAGTTT ATGATTTGAA TGATAGTGTT CAAACTATTA AGTTCTTGAT ATGGGTCATA 
AATGATATCC TCGTTCCTGC GTTTTGGCAA AGTGAGAATC CCAGCAAGCA ATTGTTTGTT 
GCCCTTGTCA TACAGGAATC ATTAAAATAT TGCGGGCTAA GTTCAGAGTC ATGGGATATG 
AACCATAAAG AATTATATCC AAATGAAGCC AAACTATGGG AAAAGTTTAA CTCTGTCTCC 
AAGACAACCA TCTATCCGCT TTTATCTTCC TTGTATCTTG CGCAATCATG GAAAGAATAT 
GTCCCGCTAA AATATCCTTC TAATAACTTC AAGGAAGGAT ACAAAATTTG GGTGAAAAGG 
TTTACATTGG ATTTATTGAA AACAGGTACA ACAGAAAATC ATCCATTACA CGTGTTTTCC 
TCTTTGATTA GGGAAGATGA TGGCTCACTA TCAAATTTTT TGCTACCTTA TATTTCTCTG 
GACATTATTA TCAAGGCAGA AAAAGGAACT CCATACGCTG ATATTTTAAA CGGGATTATT 
ATTGAATTTG ACAGCATTTT CACGTGCAAT CTGGAAGGAA TGAATAACTT GCAAGTGGAT 
TCGTTAAGAA TGTGCTATGA ATCCATCTTC AGAGTTTTCG AATATTGCAA AAAATGGGCA 
ACTGAGTTTA AACAAAATTA CAGTAAACTA CACGGCACTT TTATCATTAA AGATACGAAA 
ACAACTAACA TGCTTTTGAG AATAGATGAG TTTTTGCGAA CAACCCCTTC AGATTTGCTA 
GCTCAACGCT CCTTAGAGAC GGATTCTTTT GAAAGGTCTG CTCTATACCT TGAACAGTGC 
TATCGACAGA ATCCTCACGA TAAGAACCAA AATGGACAAC TACTGAAAAA TTTACAAATC 
ACATACGAAG AAATAGGAGA CATTGACTCA CTCGATGGTG TACTGAGAAC CTTTGCTACA 
GGAAACTTGG TTTCTAAAAT TGAAGAATTG CAATATTCTG AAAACTGGAA ACTCGCACAA 
GACTGCTTTA ATGTCCTCGG CAAATTTTCA GATGACCCCA AAACTACAAC CAGGATGCTA 
AAGTCTATGT ATGACCACCA ATTGTATTCT CAAATAATAT CGAACTCTTC GTTCCATTCT 
TCAGACGGAA AAATTTCTTT GTCTCCAGAT GTGAAGGAAT GGTACAGCAT AGGTCTTGAA 
GCTGCAAATC TAGAAGGCAA TGTTCAAACT TTGAAAAATT GGGTAGAACA AATAGAGAGT 
TTAAGAAATA TTGACGATAG AGAAGTACTT TTGCAGTACA ATATTGCGAA AGCTTTAATT 
GCCATCTCAA ACGAGGATCC ATTAAGGACT CAAAAATACA TCCACAATTC CTTTAGGCTT 
ATCGGAACAA ATTTTATAAC GTCATCTAAA GAGACGACGC TGCTAAAGAA ACAGAATTTA 
TTGATGAAAT TACACAGTTT ATATGACCTC AGTTTTTTAT CTTCTGCGAA AGATAAGTTT 
GAATACAAAA GTAACACTAC CATACTCGAT TATCGAATGG AACGTATTGG GGCTGACTTC 
GTGCCAAATC ATTACATATT GTCAATGAGA AAGTCATTTG ACCAATTGAA AATGAATGAA 
CAAGCAGACG CTGACTTAGG AAAAACATTC TTCACTTTAG CCCAATTGGC GAGAAACAAC 
GCTAGGCTAG ATATAGCCTC CGAATCATTA ATGCATTGTT TGGAAAGGCG GTTGCCTCAG 
GCAGAGTTGG AGTTTGCTGA AATACTATGG AAGCAAGGTG AGAATGATAG AGCCTTAAAG 
ATAGTGCAAG AAATACATGA AAAGTATCAA GAAAATTCCT CGGTTAATGC TCGCGATCGT 
GCCGCCGTGC TATTAAAGTT TACTGAATGG TTAGACCTTT CGAACAATTC AGCGTCCGAA 
CAAATTATTA AACAATATCA GGATATTTTT CAGATTGATT CTAAATGGGA TAAACCATAT 
TACTCTATTG GCTTATACTA TAGTAGACTA CTTGAGCGCA AAAAAGCAGA GGGTTATATT 
ACTAATGGTC GTTTTGAGTA CAGGGCAATA TCTTACTTTT TATTGGCATT TGAAAAGAAC 
ACTGCTAAAG TAAGAGAAAA TTTGCCCAAA GTTATCACGT TTTGGCTAGA TATTGCGGCC 
GCATCAATTT CTGAAGCTCC TGGAAACAGA AAGGAAATGC TGAGTAAGGC TACGGAAGAT 
ATATGTAGTC ATGTTGAAGA AGCGCTGCAG CATTGTCCCA CTTATATTTG GTACTTTGTT 
TTGACTCAGT TGTTATCTAG GTTATTACAT TCTCATCAAT CATCGGCCCA GATAATAATG 
CACATACTGC TAAGTTTGGC TGTTGAATAC CCCTCTCATA TTTTATGGTA TATCACAGCC 
CTTGTAAATT CCAATTCTTC AAAAAGAGTT CTTCGTGGTA AGCATATTTT AGAAAAGTAT 
AGACAACATT CGCAAAATCC TCATGATCTA GTTTCTAGTG CATTGGATTT AACGAAAGCA 
TTAACTCGTG TCTGTTTGCA AGATGTCAAA AGCATTACAA GTAGATCAGG CAAATCTTTA 
GAAAAAGACT TCAAATTTGA CATGAACGTG GCCCCATCTG CAATGGTTGT TCCAGTAAGA 
AAAAATTTAG ACATCATTTC ACCACTAGAG TCTAACTCAA TGAGGGGCTA TCAACCATTT 
AGGCCGGTTG TTTCTATAAT TAGATTCGGA TCATCTTATA AAGTGTTTTC TTCATTAAAG 
AAGCCAAAAC AATTGAACAT AATAGGTTCA GATGGCAACA TTTATGGGAT CATGTGTAAG 
AAGGAAGATG TCCGACAAGA TAACCAATAT ATGCAGTTCG CCACAACAAT GGATTTTCTT 
CTGAGTAAGG ACATAGCTTC AAGAAAAAGA AGCCTGGGCA TAAATATTTA CTCCGTACTA 
TCTCTTCGAG AAGACTGTGG GATATTGGAA ATGGTACCGA ATGTTGTAAC TTTAAGATCT 
ATTCTTTCTA CAAAGTACGA AAGTCTGAAA ATTAAGTATA GCCTGAAAAG TCTACATGAT 
AGGTGGCAGC ACACCGCAGT AGATGGAAAA CTCGAGTTTT ACATGGAACA GGTAGATAAA 
TTTCCTCCAA TCTTGTACCA ATGGTTTTTA GAAAACTTTC CTGATCCAAT CAATTGGTTC 
AACGCCAGGA ATACGTATGC CAGATCTTAC GCCGTCATGG CAATGGTTGG CCATATATTA 
GGTCTAGGTG ATAGGCACTG TGAAAACATA TTACTAGATA TACAGACGGG TAAAGTTCTT 
CATGTAGACT TCGACTGTTT ATTTGAGAAA GGCAAAAGGT TACCTGTCCC AGAAATTGTT 
CCCTTCAGAC TAACACCAAA TTTATTGGAT GCGTTGGGCA TAATTGGGAC AGAAGGAACA 
TTTAAGAAGT CTAGTGAAGT CACGTTGGCT TTAATGAGAA AAAATGAAGT AGCGTTGATG 
AATGTGATCG AAACAATTAT GTACGATAGA AACATGGACC ACTCAATTCA AAAAGCGCTA 
AAGGTCTTAA GAAACAAAAT CCGCGGTATA GATCCGCAGG ATGGCCTGGT ATTGAGTGTT 
GCTGGCCAAA CAGAAACATT GATCCAAGAA GCAACATCAG AAGACAATCT AAGCAAGATG 
TATATTGGTT GGCTTCCATT TTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_009694.3
CDS	1..7107
Translation	MESHVKYLDELILAIKDLNSGVDSKVQIKKVPTDPSSSQEYAKSLKILNTLIRNLKDQRRNNIMKNDTIFSKTVSALALLLEYNPFLLVMKDSNGNFEIQRLIDDFLNISVLNYDNYHRIWFMRRKLGSWCKACVEFYGKPAKFQLTAHFENTMNLYEQALTEVLLGKTELLKFYDTLKGLYILLYWFTSEYSTFGNSIAFLDSSLGFTKFDFNFQRLIRIVLYVFDSCELAALEYAEIQLKYISLVVDYVCNRTISTALDAPALVCCEQLKFVLTTMHHFLDNKYGLLDNDPTMAKGILRLYSLCISNDFSKCFVDHFPIDQWADFSQSEHFPFTQLTNKALSIVYFDLKRRSLPVEALKYDNKFNIWVYQSEPDSSLKNVTSPFDDRYKQLEKLRLLVLKKFNKTERGTLLKYRVNQLSPGFFQRAGNDFKLILNEASVSIQTCFKTNNITRLTSWTVILGRLACLESEKFSGTLPNSTKDMDNWYVCHLCDIEKTGNPFVRINPNRPEAAGKSEIFRILHSNFLSHPNIDEFSESLLSGILFSLHRIFSHFQPPKLTDGNGQINKSFKLVQKCFMNSNRYLRLLSTRIIPLFNISDSHNSEDEHTATLIKFLQSQKLPVVKENLVIAWTQLTLTTSNDVFDTLLLKLIDIFNSDDYSLRIMMTLQIKNMAKILKKTPYQLLSPILPVLLRQLGKNLVERKVGFQNLIELLGYSSKTILDIFQRYIIPYAIIQYKSDVLSEIAKIMCDGDTSLINQMKVNLLKKNSRQIFAVALVKHGLFSLDILETLFLNRAPTFDKGYITAYLPDYKTLAEITKLYKNSVTKDASDSENANMILCSLRFLITNFEKDKRHGSKYKNINNWTDDQEQAFQKKLQDNILGIFQVFSSDIHDVEGRTTYYEKLRVINGISFLIIYAPKKSIISALAQISICLQTGLGLKEVRYEAFRCWHLLVRHLNDEELSTVIDSLIAFILQKWSEFNGKLRNIVYSILDTLIKEKSDLILKLKPYTTLALVGKPELGILARDGQFARMVNKIRSTTDLIPIFANNLKSSNKYVINQNLDDIEVYLRRKQTERSIDFTPKKVGQTSDITLVLGALLDTSHKFRNLDKDLCEKCAKCISMIGVLDVTKHEFKRTTYSENEVYDLNDSVQTIKFLIWVINDILVPAFWQSENPSKQLFVALVIQESLKYCGLSSESWDMNHKELYPNEAKLWEKFNSVSKTTIYPLLSSLYLAQSWKEYVPLKYPSNNFKEGYKIWVKRFTLDLLKTGTTENHPLHVFSSLIREDDGSLSNFLLPYISLDIIIKAEKGTPYADILNGIIIEFDSIFTCNLEGMNNLQVDSLRMCYESIFRVFEYCKKWATEFKQNYSKLHGTFIIKDTKTTNMLLRIDEFLRTTPSDLLAQRSLETDSFERSALYLEQCYRQNPHDKNQNGQLLKNLQITYEEIGDIDSLDGVLRTFATGNLVSKIEELQYSENWKLAQDCFNVLGKFSDDPKTTTRMLKSMYDHQLYSQIISNSSFHSSDGKISLSPDVKEWYSIGLEAANLEGNVQTLKNWVEQIESLRNIDDREVLLQYNIAKALIAISNEDPLRTQKYIHNSFRLIGTNFITSSKETTLLKKQNLLMKLHSLYDLSFLSSAKDKFEYKSNTTILDYRMERIGADFVPNHYILSMRKSFDQLKMNEQADADLGKTFFTLAQLARNNARLDIASESLMHCLERRLPQAELEFAEILWKQGENDRALKIVQEIHEKYQENSSVNARDRAAVLLKFTEWLDLSNNSASEQIIKQYQDIFQIDSKWDKPYYSIGLYYSRLLERKKAEGYITNGRFEYRAISYFLLAFEKNTAKVRENLPKVITFWLDIAAASISEAPGNRKEMLSKATEDICSHVEEALQHCPTYIWYFVLTQLLSRLLHSHQSSAQIIMHILLSLAVEYPSHILWYITALVNSNSSKRVLRGKHILEKYRQHSQNPHDLVSSALDLTKALTRVCLQDVKSITSRSGKSLEKDFKFDMNVAPSAMVVPVRKNLDIISPLESNSMRGYQPFRPVVSIIRFGSSYKVFSSLKKPKQLNIIGSDGNIYGIMCKKEDVRQDNQYMQFATTMDFLLSKDIASRKRSLGINIYSVLSLREDCGILEMVPNVVTLRSILSTKYESLKIKYSLKSLHDRWQHTAVDGKLEFYMEQVDKFPPILYQWFLENFPDPINWFNARNTYARSYAVMAMVGHILGLGDRHCENILLDIQTGKVLHVDFDCLFEKGKRLPVPEIVPFRLTPNLLDALGIIGTEGTFKKSSEVTLALMRKNEVALMNVIETIMYDRNMDHSIQKALKVLRNKIRGIDPQDGLVLSVAGQTETLIQEATSEDNLSKMYIGWLPFW

Target ORF information:

RefSeq Version	NM_001178484.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C protein kinase MEC1 (MEC1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178484.3

ORF Insert Sequence:

ATGGAATCAC ACGTCAAATA TCTTGACGAA TTGATATTGG CAATAAAAGA CCTGAACTCG 
GGGGTGGATT CAAAGGTGCA GATTAAAAAA GTGCCCACGG ATCCATCTTC TTCTCAGGAG 
TACGCCAAGA GTTTAAAGAT CCTGAACACC CTCATAAGAA ACCTAAAAGA TCAAAGAAGG 
AACAATATCA TGAAAAATGA TACTATATTT TCGAAAACAG TTTCCGCCCT TGCCTTATTG 
TTGGAGTACA ACCCCTTCTT GCTTGTTATG AAGGATTCCA ACGGGAACTT TGAGATACAA 
AGGCTGATAG ATGATTTCCT CAACATATCC GTTCTGAACT ATGATAATTA CCACAGAATA 
TGGTTTATGA GGCGAAAATT AGGCAGCTGG TGCAAAGCAT GTGTCGAATT TTACGGAAAA 
CCTGCTAAGT TTCAGCTTAC TGCACATTTT GAGAACACCA TGAATCTTTA CGAACAGGCC 
TTGACTGAAG TCTTGTTGGG CAAGACTGAG CTTCTCAAAT TTTATGACAC CTTGAAGGGT 
CTATACATTC TTTTATACTG GTTCACTTCG GAGTATAGTA CTTTTGGGAA CTCTATAGCA 
TTCTTAGATT CTTCTTTGGG GTTCACGAAA TTTGACTTTA ACTTCCAACG ATTAATCAGG 
ATTGTTCTTT ACGTCTTTGA TTCCTGCGAA CTAGCAGCAC TAGAATATGC CGAAATCCAA 
CTCAAATATA TTTCTCTAGT TGTGGACTAT GTTTGCAATA GAACAATTTC CACAGCCCTG 
GATGCCCCAG CGTTAGTTTG TTGTGAACAA TTAAAGTTTG TATTGACTAC TATGCATCAT 
TTTTTGGATA ACAAGTATGG GCTCTTGGAT AATGACCCCA CTATGGCCAA AGGAATTCTT 
CGACTATATT CTCTTTGCAT TTCTAACGAT TTCTCAAAAT GCTTTGTAGA CCACTTCCCA 
ATTGACCAGT GGGCAGATTT TTCACAAAGT GAACATTTTC CGTTCACGCA GTTGACTAAT 
AAAGCTCTCT CGATTGTATA TTTTGATTTG AAAAGAAGGT CCCTACCTGT TGAAGCTTTA 
AAGTACGATA ATAAGTTCAA CATCTGGGTA TACCAATCGG AGCCGGACTC GAGCTTGAAA 
AATGTCACTT CTCCCTTTGA TGATCGATAT AAGCAGCTGG AAAAGCTAAG GTTGCTAGTA 
CTAAAGAAGT TTAACAAGAC AGAAAGAGGA ACTTTGCTCA AATACCGCGT GAACCAGCTA 
AGTCCTGGAT TTTTTCAAAG AGCTGGAAAC GATTTCAAGC TAATTTTAAA TGAAGCATCT 
GTATCCATTC AAACTTGTTT CAAGACAAAC AATATAACAA GGCTAACATC ATGGACTGTA 
ATTCTCGGAC GTCTAGCCTG TCTAGAATCA GAGAAGTTTT CCGGCACTCT GCCAAATTCC 
ACAAAGGATA TGGATAATTG GTATGTTTGT CATTTATGCG ATATTGAGAA AACTGGCAAC 
CCTTTCGTGC GAATAAATCC AAATAGACCA GAGGCTGCGG GTAAATCAGA AATCTTCAGG 
ATACTTCATT CAAACTTTCT ATCTCACCCA AATATAGATG AATTTAGCGA ATCTTTGTTA 
AGTGGCATCT TATTTTCTCT ACATAGGATA TTTTCACACT TTCAACCTCC AAAACTTACA 
GATGGAAACG GTCAAATCAA TAAGAGCTTT AAACTGGTAC AAAAGTGCTT TATGAATTCT 
AACAGATACC TACGTTTATT AAGTACTAGA ATTATACCTT TATTCAATAT ATCAGACTCT 
CATAATTCCG AAGATGAACA CACTGCCACG CTGATAAAGT TTCTACAATC TCAAAAATTG 
CCAGTGGTGA AAGAAAATTT AGTCATTGCT TGGACACAAT TAACATTGAC GACTTCTAAT 
GATGTATTTG ATACACTACT TTTGAAACTG ATTGATATTT TCAATTCTGA TGATTATAGT 
TTACGAATAA TGATGACTTT GCAAATTAAA AATATGGCCA AAATTTTAAA GAAAACACCA 
TATCAATTAC TATCGCCTAT TTTACCTGTA TTACTAAGAC AGTTGGGAAA AAACCTCGTG 
GAAAGAAAAG TTGGCTTTCA AAATTTAATA GAATTATTGG GATATTCTTC AAAAACAATT 
CTCGATATTT TCCAGAGATA TATCATCCCT TATGCAATTA TTCAATATAA GAGCGATGTG 
CTAAGTGAAA TTGCTAAGAT TATGTGTGAT GGCGATACAA GTTTAATTAA CCAAATGAAG 
GTTAATTTAC TGAAAAAAAA CAGTAGGCAA ATATTTGCCG TAGCTTTGGT AAAACACGGA 
TTATTTTCTT TGGATATCTT GGAAACCCTT TTTTTAAATA GGGCTCCAAC TTTTGACAAA 
GGATATATAA CTGCATACCT TCCCGATTAT AAAACTTTAG CTGAAATAAC GAAGCTCTAC 
AAAAACAGCG TTACTAAAGA TGCAAGTGAC AGCGAGAATG CTAATATGAT TTTATGCTCT 
TTGCGATTTT TAATCACCAA TTTTGAAAAA GACAAAAGGC ATGGTTCGAA GTACAAAAAT 
ATCAATAACT GGACGGATGA TCAGGAACAA GCGTTCCAAA AGAAACTACA GGATAATATC 
TTAGGTATTT TCCAAGTTTT TTCGAGTGAC ATACATGATG TTGAAGGCCG CACCACTTAC 
TACGAAAAGT TAAGGGTTAT CAATGGCATT TCTTTTCTTA TCATATATGC CCCCAAAAAA 
TCAATAATTT CCGCATTAGC CCAGATTAGT ATTTGTTTGC AAACAGGACT TGGGCTTAAG 
GAAGTTCGAT ACGAGGCCTT TAGATGTTGG CATCTGTTAG TTCGCCATCT AAATGATGAA 
GAACTCTCTA CTGTTATAGA TAGCTTAATT GCATTCATAC TTCAAAAGTG GTCTGAGTTC 
AACGGAAAAC TTCGAAATAT AGTGTACAGT ATACTGGATA CCTTAATCAA AGAGAAATCG 
GACCTGATTT TGAAATTAAA ACCTTACACT ACTTTGGCTT TAGTAGGCAA GCCTGAATTA 
GGTATTTTAG CTCGTGATGG CCAATTTGCA AGAATGGTGA ATAAAATAAG AAGTACCACG 
GACCTTATAC CCATATTTGC TAATAACTTG AAAAGTAGTA ACAAGTATGT CATAAACCAA 
AATTTAGACG ATATAGAGGT ATATCTTCGG AGAAAGCAGA CAGAAAGATC GATTGATTTT 
ACACCAAAGA AGGTTGGGCA AACTTCTGAT ATAACATTAG TTTTGGGTGC TTTATTAGAC 
ACTTCTCATA AGTTTAGAAA TTTAGACAAG GACCTATGCG AGAAGTGCGC CAAATGTATC 
AGTATGATTG GTGTTTTAGA CGTTACAAAG CATGAGTTTA AAAGAACAAC ATATTCAGAA 
AACGAAGTTT ATGATTTGAA TGATAGTGTT CAAACTATTA AGTTCTTGAT ATGGGTCATA 
AATGATATCC TCGTTCCTGC GTTTTGGCAA AGTGAGAATC CCAGCAAGCA ATTGTTTGTT 
GCCCTTGTCA TACAGGAATC ATTAAAATAT TGCGGGCTAA GTTCAGAGTC ATGGGATATG 
AACCATAAAG AATTATATCC AAATGAAGCC AAACTATGGG AAAAGTTTAA CTCTGTCTCC 
AAGACAACCA TCTATCCGCT TTTATCTTCC TTGTATCTTG CGCAATCATG GAAAGAATAT 
GTCCCGCTAA AATATCCTTC TAATAACTTC AAGGAAGGAT ACAAAATTTG GGTGAAAAGG 
TTTACATTGG ATTTATTGAA AACAGGTACA ACAGAAAATC ATCCATTACA CGTGTTTTCC 
TCTTTGATTA GGGAAGATGA TGGCTCACTA TCAAATTTTT TGCTACCTTA TATTTCTCTG 
GACATTATTA TCAAGGCAGA AAAAGGAACT CCATACGCTG ATATTTTAAA CGGGATTATT 
ATTGAATTTG ACAGCATTTT CACGTGCAAT CTGGAAGGAA TGAATAACTT GCAAGTGGAT 
TCGTTAAGAA TGTGCTATGA ATCCATCTTC AGAGTTTTCG AATATTGCAA AAAATGGGCA 
ACTGAGTTTA AACAAAATTA CAGTAAACTA CACGGCACTT TTATCATTAA AGATACGAAA 
ACAACTAACA TGCTTTTGAG AATAGATGAG TTTTTGCGAA CAACCCCTTC AGATTTGCTA 
GCTCAACGCT CCTTAGAGAC GGATTCTTTT GAAAGGTCTG CTCTATACCT TGAACAGTGC 
TATCGACAGA ATCCTCACGA TAAGAACCAA AATGGACAAC TACTGAAAAA TTTACAAATC 
ACATACGAAG AAATAGGAGA CATTGACTCA CTCGATGGTG TACTGAGAAC CTTTGCTACA 
GGAAACTTGG TTTCTAAAAT TGAAGAATTG CAATATTCTG AAAACTGGAA ACTCGCACAA 
GACTGCTTTA ATGTCCTCGG CAAATTTTCA GATGACCCCA AAACTACAAC CAGGATGCTA 
AAGTCTATGT ATGACCACCA ATTGTATTCT CAAATAATAT CGAACTCTTC GTTCCATTCT 
TCAGACGGAA AAATTTCTTT GTCTCCAGAT GTGAAGGAAT GGTACAGCAT AGGTCTTGAA 
GCTGCAAATC TAGAAGGCAA TGTTCAAACT TTGAAAAATT GGGTAGAACA AATAGAGAGT 
TTAAGAAATA TTGACGATAG AGAAGTACTT TTGCAGTACA ATATTGCGAA AGCTTTAATT 
GCCATCTCAA ACGAGGATCC ATTAAGGACT CAAAAATACA TCCACAATTC CTTTAGGCTT 
ATCGGAACAA ATTTTATAAC GTCATCTAAA GAGACGACGC TGCTAAAGAA ACAGAATTTA 
TTGATGAAAT TACACAGTTT ATATGACCTC AGTTTTTTAT CTTCTGCGAA AGATAAGTTT 
GAATACAAAA GTAACACTAC CATACTCGAT TATCGAATGG AACGTATTGG GGCTGACTTC 
GTGCCAAATC ATTACATATT GTCAATGAGA AAGTCATTTG ACCAATTGAA AATGAATGAA 
CAAGCAGACG CTGACTTAGG AAAAACATTC TTCACTTTAG CCCAATTGGC GAGAAACAAC 
GCTAGGCTAG ATATAGCCTC CGAATCATTA ATGCATTGTT TGGAAAGGCG GTTGCCTCAG 
GCAGAGTTGG AGTTTGCTGA AATACTATGG AAGCAAGGTG AGAATGATAG AGCCTTAAAG 
ATAGTGCAAG AAATACATGA AAAGTATCAA GAAAATTCCT CGGTTAATGC TCGCGATCGT 
GCCGCCGTGC TATTAAAGTT TACTGAATGG TTAGACCTTT CGAACAATTC AGCGTCCGAA 
CAAATTATTA AACAATATCA GGATATTTTT CAGATTGATT CTAAATGGGA TAAACCATAT 
TACTCTATTG GCTTATACTA TAGTAGACTA CTTGAGCGCA AAAAAGCAGA GGGTTATATT 
ACTAATGGTC GTTTTGAGTA CAGGGCAATA TCTTACTTTT TATTGGCATT TGAAAAGAAC 
ACTGCTAAAG TAAGAGAAAA TTTGCCCAAA GTTATCACGT TTTGGCTAGA TATTGCGGCC 
GCATCAATTT CTGAAGCTCC TGGAAACAGA AAGGAAATGC TGAGTAAGGC TACGGAAGAT 
ATATGTAGTC ATGTTGAAGA AGCGCTGCAG CATTGTCCCA CTTATATTTG GTACTTTGTT 
TTGACTCAGT TGTTATCTAG GTTATTACAT TCTCATCAAT CATCGGCCCA GATAATAATG 
CACATACTGC TAAGTTTGGC TGTTGAATAC CCCTCTCATA TTTTATGGTA TATCACAGCC 
CTTGTAAATT CCAATTCTTC AAAAAGAGTT CTTCGTGGTA AGCATATTTT AGAAAAGTAT 
AGACAACATT CGCAAAATCC TCATGATCTA GTTTCTAGTG CATTGGATTT AACGAAAGCA 
TTAACTCGTG TCTGTTTGCA AGATGTCAAA AGCATTACAA GTAGATCAGG CAAATCTTTA 
GAAAAAGACT TCAAATTTGA CATGAACGTG GCCCCATCTG CAATGGTTGT TCCAGTAAGA 
AAAAATTTAG ACATCATTTC ACCACTAGAG TCTAACTCAA TGAGGGGCTA TCAACCATTT 
AGGCCGGTTG TTTCTATAAT TAGATTCGGA TCATCTTATA AAGTGTTTTC TTCATTAAAG 
AAGCCAAAAC AATTGAACAT AATAGGTTCA GATGGCAACA TTTATGGGAT CATGTGTAAG 
AAGGAAGATG TCCGACAAGA TAACCAATAT ATGCAGTTCG CCACAACAAT GGATTTTCTT 
CTGAGTAAGG ACATAGCTTC AAGAAAAAGA AGCCTGGGCA TAAATATTTA CTCCGTACTA 
TCTCTTCGAG AAGACTGTGG GATATTGGAA ATGGTACCGA ATGTTGTAAC TTTAAGATCT 
ATTCTTTCTA CAAAGTACGA AAGTCTGAAA ATTAAGTATA GCCTGAAAAG TCTACATGAT 
AGGTGGCAGC ACACCGCAGT AGATGGAAAA CTCGAGTTTT ACATGGAACA GGTAGATAAA 
TTTCCTCCAA TCTTGTACCA ATGGTTTTTA GAAAACTTTC CTGATCCAAT CAATTGGTTC 
AACGCCAGGA ATACGTATGC CAGATCTTAC GCCGTCATGG CAATGGTTGG CCATATATTA 
GGTCTAGGTG ATAGGCACTG TGAAAACATA TTACTAGATA TACAGACGGG TAAAGTTCTT 
CATGTAGACT TCGACTGTTT ATTTGAGAAA GGCAAAAGGT TACCTGTCCC AGAAATTGTT 
CCCTTCAGAC TAACACCAAA TTTATTGGAT GCGTTGGGCA TAATTGGGAC AGAAGGAACA 
TTTAAGAAGT CTAGTGAAGT CACGTTGGCT TTAATGAGAA AAAATGAAGT AGCGTTGATG 
AATGTGATCG AAACAATTAT GTACGATAGA AACATGGACC ACTCAATTCA AAAAGCGCTA 
AAGGTCTTAA GAAACAAAAT CCGCGGTATA GATCCGCAGG ATGGCCTGGT ATTGAGTGTT 
GCTGGCCAAA CAGAAACATT GATCCAAGAA GCAACATCAG AAGACAATCT AAGCAAGATG 
TATATTGGTT GGCTTCCATT TTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete DNA sequence of yeast chromosome II.
The EMBO journal13(24)5795-809(1994 Dec)
Feldmann H,Aigle M,Aljinovic G,Andr? B,Baclet MC,Barthe C,Baur A,B?cam AM,Biteau N,Boles E,Brandt T,Brendel M,Br?ckner M,Bussereau F,Christiansen C,Contreras R,Crouzet M,Cziepluch C,D?molis N,Delaveau T,Doignon F,Domdey H,D?sterhus S,Dubois E,Dujon B,El Bakkoury M,Entian KD,Feurmann M,Fiers W,Fobo GM,Fritz C,Gassenhuber H,Glandsdorff N,Goffeau A,Grivell LA,de Haan M,Hein C,Herbert CJ,Hollenberg CP,Holmstr?m K,Jacq C,Jacquet M,Jauniaux JC,Jonniaux JL,Kalles?e T,Kiesau P,Kirchrath L,K?tter P,Korol S,Liebl S,Logghe M,Lohan AJ,Louis EJ,Li ZY,Maat MJ,Mallet L,Mannhaupt G,Messenguy F,Miosga T,Molemans F,M?ller S,Nasr F,Obermaier B,Perea J,Pi?rard A,Piravandi E,Pohl FM,Pohl TM,Potier S,Proft M,Purnelle B,Ramezani Rad M,Rieger M,Rose M,Schaaff-Gerstenschl?ger I,Scherens B,Schwarzlose C,Skala J,Slonimski PP,Smits PH,Souciet JL,Steensma HY,Stucka R,Urrestarazu A,van der Aart QJ,van Dyck L,Vassarotti A,Vetter I,Vierendeels F,Vissers S,Wagner G,de Wergifosse P,Wolfe KH,Zagulski M,Zimmermann FK,Mewes HW,Kleine K

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MEC1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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