GCN2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	GCN2
Entrez Gene ID	851877
Full Name	serine/threonine-protein kinase GCN2
Synonyms	AAS1,AAS102,NDR2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001180591.3	NP_010569.3	serine/threonine-protein kinase GCN2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EIF2AK4	XP_001140245.1
	EIF2AK4	XP_002804763.1
Gallus gallus (chicken)	EIF2AK4	XP_004941777.1
Eremothecium gossypii	AGOS_AAL083W	NP_982459.1
Rattus norvegicus (Norway rat)	Eif2ak4	NP_001099214.2
Danio rerio (zebrafish)	eif2ak4	XP_002667149.3
Drosophila melanogaster (fruit fly)	Gcn2	NP_001263141.1
Saccharomyces cerevisiae (baker's yeast)	GCN2	NP_010569.3
Kluyveromyces lactis	KLLA0C10802g	XP_452681.1
Neurospora crassa	NCU01187	XP_961553.1
Xenopus tropicalis (tropical clawed frog)	eif2ak4	NP_001243855.1
Homo sapiens (human)	EIF2AK4	NP_001013725.2
Canis lupus familiaris (dog)	EIF2AK4	XP_535431.3
Bos taurus (cattle)	EIF2AK4	XP_002690831.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012368	XP_320188.4
Caenorhabditis elegans (roundworm)	gcn-2	NP_001254370.1
Schizosaccharomyces pombe (fission yeast)	gcn2	NP_595991.2
Mus musculus (house mouse)	Eif2ak4	NP_038747.2
Magnaporthe oryzae (rice blast fungus)	MGG_08643	XP_003711074.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Jacq C, Alt-M?rbe J, Andre B, Arnold W, Bahr A, Ballesta JP, Bargues M, Baron L, Becker A, Biteau N, Bl?cker H, Blugeon C, Boskovic J, Brandt P, Br?ckner M, Buitrago MJ, Coster F, Delaveau T, del Rey F, Dujon B, Eide LG, Garcia-Cantalejo JM, Goffeau A, Gomez-Peris A, Zaccaria P
Nature387(6632 Suppl)75-8(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GCN2 is required for nitrogen catabolite repression-sensitive nuclear Gln3 localization. Gcn2 functions upstream of Ure2.
Title: General Amino Acid Control and 14-3-3 Proteins Bmh1/2 Are Required for Nitrogen Catabolite Repression-Sensitive Regulation of Gln3 and Gat1 Localization.

findings demonstrate that TORC1 is an effector of Gcn2 in amino acid signaling, hence defining a novel mechanism by which TORC1 senses amino acid starvation
Title: General Control Nonderepressible 2 (GCN2) Kinase Inhibits Target of Rapamycin Complex 1 in Response to Amino Acid Starvation in

To mediate the activation of protein kinase Gcn2, Gcn1 interacts with Rps10.
Title: Gcn1 contacts the small ribosomal protein Rps10, which is required for full activation of the protein kinase Gcn2.

Mutations leading to amino acid substitutions on this surface were identified that activate Gcn2 at low levels of tRNA binding (Gcd- phenotype), while other substitutions block kinase activation (Gcn- phenotype
Title: Interaction between the tRNA-binding and C-terminal domains of Yeast Gcn2 regulates kinase activity in vivo.

Gcd- substitutions enhance YKD-KD interactions in vitro, whereas Gcn- substitutions in alphaE and alphaI suppress both this effect and the constitutive activation of Gcn2 conferred by YKD Gcd- substitutions
Title: Enhanced interaction between pseudokinase and kinase domains in Gcn2 stimulates eIF2? phosphorylation in starved cells.

The following GCN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi01361	NM_001180591.3 Latest version!	Saccharomyces cerevisiae S288C serine/threonine-protein kinase GCN2 (GCN2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCN2 ( NM_001180591.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_010569.3 Saccharomyces cerevisiae S288C serine/threonine-protein kinase GCN2 (GCN2), partial mRNA.

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CloneID	OSi01361
Clone ID Related Accession (Same CDS sequence)	NM_001180591.3
Accession Version	NM_001180591.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4980bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	serine/threonine-protein kinase GCN2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001136). On Jul 30, 2012 this sequence version replaced NM_001180591.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCATTGA GTCATCTCAC TTTAGATCAA TATTATGAAA TACAGTGCAA CGAACTTGAA 
GCGATACGTT CCATTTATAT GGATGACTTT ACTGACTTAA CTAAAAGAAA GTCTAGCTGG 
GATAAGCAGC CACAGATTAT ATTCGAAATT ACGCTTCGAT CTGTTGACAA AGAGCCGGTT 
GAATCTTCTA TAACATTACA TTTTGCGATG ACCCCAATGT ATCCTTATAC CGCTCCAGAA 
ATAGAATTCA AAAATGTACA AAATGTAATG GATAGTCAAT TGCAAATGCT GAAAAGTGAA 
TTTAAGAAAA TCCACAACAC CTCCCGAGGC CAAGAGATTA TATTTGAAAT TACATCTTTT 
ACTCAAGAAA AACTGGACGA ATTTCAAAAT GTGGTAAATA CACAGTCCTT GGAAGATGAT 
CGATTACAAA GAATCAAAGA AACTAAAGAA CAATTAGAAA AGGAAGAAAG AGAAAAACAA 
CAGGAAACAA TAAAAAAAAG ATCAGATGAG CAGCGAAGGA TAGATGAAAT TGTTCAAAGA 
GAGTTGGAGA AAAGACAAGA TGATGATGAT GATTTGCTAT TCAACAGAAC AACCCAGTTA 
GATTTACAAC CACCTTCAGA ATGGGTTGCA TCAGGTGAAG CTATTGTCTT TTCAAAAACT 
ATAAAGGCAA AATTGCCTAA TAACTCAATG TTCAAGTTTA AAGCAGTTGT AAATCCTAAG 
CCAATAAAAC TGACATCAGA TATATTTAGT TTTTCCAAAC AATTTCTTGT GAAGCCTTAT 
ATACCACCAG AATCTCCGTT GGCAGATTTT TTAATGTCTT CTGAAATGAT GGAAAATTTC 
TACTATTTGC TATCTGAAAT TGAATTGGAT AATAGCTATT TCAACACAAG TAATGGAAAA 
AAAGAAATAG CAAATTTAGA GAAAGAGTTA GAGACGGTGT TGAAAGCTAA GCATGACAAT 
GTGAATCGAT TGTTCGGTTA TACGGTGGAG CGCATGGGGA GAAATAATGC AACGTTTGTT 
TGGAAAATAA GACTCTTGAC AGAGTACTGT AACTACTATC CATTGGGAGA TTTGATACAA 
TCTGTTGGAT TTGTTAACTT AGCAACAGCG CGTATTTGGA TGATTAGATT GCTTGAAGGA 
TTGGAGGCCA TACACAAATT GGGAATTGTT CATAAATGTA TCAACTTAGA AACCGTGATC 
CTGGTGAAGG ATGCAGATTT TGGAAGCACT ATTCCCAAGT TAGTTCACTC CACTTATGGC 
TACACTGTTT TGAATATGCT ATCGAGATAT CCAAATAAAA ATGGTTCTTC GGTTGAGTTA 
TCTCCAAGTA CATGGATAGC CCCTGAGTTG TTGAAATTCA ATAACGCCAA ACCTCAAAGA 
TTAACTGATA TTTGGCAACT TGGTGTTTTG TTTATCCAGA TAATCAGTGG ATCTGATATA 
GTGATGAATT TTGAAACGCC TCAAGAATTC CTAGATTCAA CAAGTATGGA TGAAACTTTA 
TATGATCTTC TTTCGAAAAT GCTTAATAAC GATCCGAAGA AAAGATTAGG AACATTAGAA 
CTACTGCCCA TGAAATTCTT AAGGACCAAT ATTGACTCTA CAATCAATCG ATTTAACTTA 
GTTTCCGAAA GTGTCAATTC TAATTCCTTG GAGTTAACTC CTGGAGATAC CATAACCGTT 
CGGGGCAATG GAGGTAGAAC ACTTTCACAA TCGAGTATAC GAAGAAGATC ATTTAATGTT 
GGTTCCAGAT TCTCTTCTAT AAATCCTGCA ACGCGATCAC GATATGCTTC TGACTTTGAA 
GAGATTGCAG TTTTAGGCCA GGGCGCATTT GGACAAGTTG TCAAGGCACG TAATGCTCTC 
GATAGCAGAT ACTATGCGAT CAAGAAGATT AGACATACAG AAGAAAAGTT ATCTACTATA 
TTGAGTGAAG TAATGCTGTT AGCAAGCTTA AATCATCAAT ATGTTGTGCG TTACTATGCT 
GCATGGTTAG AAGAAGACAG TATGGATGAA AACGTTTTTG AATCAACTGA TGAAGAAAGT 
GACTTGAGCG AATCTTCCTC TGATTTTGAG GAAAATGATT TATTAGATCA AAGCAGTATT 
TTTAAAAATA GAACAAATCA CGATTTGGAT AATAGTAACT GGGATTTCAT ATCGGGGTCA 
GGATATCCGG ATATTGTCTT TGAAAATAGT TCTCGTGATG ATGAAAATGA AGATCTAGAC 
CATGATACTT CCTCGACTTC CTCGAGCGAA AGTCAAGATG ATACTGATAA AGAATCAAAG 
AGTATCCAGA ACGTTCCAAG AAGGAGGAAT TTTGTAAAAC CGATGACTGC TGTTAAGAAG 
AAAAGTACGC TTTTTATTCA AATGGAGTAC TGTGAAAATA GAACGCTATA TGATTTGATC 
CATTCTGAAA ATTTAAATCA ACAACGTGAT GAATATTGGA GGTTATTTCG ACAAATTTTG 
GAAGCACTGA GTTATATACA TTCCCAGGGT ATCATTCATA GGGATCTGAA GCCAATGAAT 
ATTTTTATAG ATGAATCGAG AAATGTTAAA ATCGGTGATT TTGGGTTAGC TAAGAACGTC 
CATAGATCTC TGGATATACT TAAGCTAGAT TCACAGAATT TGCCAGGCAG CTCAGATAAT 
TTAACATCCG CCATTGGTAC AGCAATGTAT GTTGCTACTG AAGTTTTAGA TGGTACAGGT 
CACTATAATG AAAAGATTGA TATGTATTCA CTTGGAATCA TTTTTTTTGA AATGATCTAT 
CCTTTCAGTA CAGGTATGGA GAGAGTTAAT ATTTTGAAAA AGTTACGATC AGTGTCGATA 
GAATTTCCTC CTGATTTCGA CGATAATAAG ATGAAAGTTG AAAAGAAAAT TATAAGGTTA 
CTCATAGACC ATGATCCCAA TAAAAGGCCT GGTGCTAGGA CATTATTAAA TAGTGGTTGG 
CTTCCTGTGA AGCATCAGGA TGAAGTAATC AAAGAGGCTT TAAAAAGTTT GTCGAATCCT 
TCATCCCCTT GGCAACAGCA AGTTCGAGAA AGTTTATTTA ACCAATCTTA CAGTCTAACA 
AATGATATTC TATTTGATAA CTCAGTTCCA ACATCCACTC CTTTCGCAAA CATTCTCAGG 
TCCCAAATGA CAGAAGAGGT AGTTAAAATT TTCAGGAAAC ATGGAGGAAT TGAAAATAAT 
GCTCCTCCGA GGATTTTTCC AAAGGCCCCC ATATACGGTA CGCAGAATGT ATATGAAGTG 
CTTGACAAGG GCGGTACCGT CTTGCAGTTA CAATATGATT TAACTTATCC TATGGCTAGG 
TATCTATCTA AAAATCCAAG TCTGATTTCT AAGCAATATA GGATGCAGCA CGTTTACCGA 
CCTCCTGATC ATTCAAGGTC AAGTTTGGAA CCTAGAAAGT TTGGTGAGAT TGACTTCGAC 
ATAATTTCAA AATCTTCCTC AGAGTCAGGA TTTTATGATG CAGAAAGCTT GAAAATTATC 
GATGAAATAT TAACCGTATT TCCTGTATTT GAGAAAACAA ACACTTTTTT CATATTAAAT 
CATGCTGATA TTTTGGAGAG TGTTTTCAAC TTTACAAATA TTGATAAAGC CCAAAGGCCT 
CTAGTTTCAC GAATGTTGTC GCAAGTAGGC TTTGCAAGGT CCTTCAAGGA AGTAAAGAAT 
GAACTAAAGG CGCAACTGAA CATATCTTCT ACGGCATTGA ATGATTTGGA GTTATTTGAT 
TTTAGACTGG ACTTCGAAGC AGCCAAAAAA CGCCTGTATA AATTGATGAT TGATAGTCCG 
CATCTAAAAA AAATTGAGGA CTCTTTGTCC CATATATCAA AGGTTCTCAG TTACCTAAAA 
CCCTTAGAAG TTGCAAGAAA TGTTGTGATA TCTCCTTTGA GTAACTACAA TAGCGCTTTT 
TACAAAGGAG GTATCATGTT TCATGCAGTT TATGACGATG GATCCTCACG TAATATGATA 
GCTGCTGGAG GGAGGTATGA CACTTTGATA TCCTTTTTTG CCAGACCATC AGGAAAAAAG 
AGCAGCAATA CTCGTAAGGC TGTAGGTTTC AACTTAGCGT GGGAAACAAT ATTCGGTATA 
GCCCAAAACT ATTTCAAACT CGCTTCTGGA AATAGGATAA AGAAGAGAAA TAGGTTTTTG 
AAAGATACAG CTGTTGATTG GAAGCCAAGC AGGTGTGATG TATTGATATC GAGTTTTTCG 
AACTCTTTGT TGGACACAAT CGGGGTTACA ATACTGAATA CATTGTGGAA GCAAAACATT 
AAAGCGGATA TGTTAAGGGA TTGTTCCTCG GTGGATGATG TCGTTACTGG CGCTCAACAG 
GATGGTATAG ACTGGATTTT GCTGATTAAG CAACAAGCGT ATCCACTAAC CAATCACAAG 
AGAAAGTACA AGCCATTAAA AATAAAAAAA TTGAGCACTA ATGTTGACAT AGATTTAGAT 
CTTGATGAGT TTTTAACCTT GTACCAACAA GAAACTGGTA ATAAATCTTT GATCAACGAT 
AGTCTCACTT TGGGCGATAA GGCTGATGAA TTTAAAAGAT GGGATGAAAA CAGCAGTGCC 
GGTAGTAGTC AAGAAGGTGA CATAGATGAT GTTGTTGCTG GTTCGACTAA TAATCAAAAG 
GTAATTTATG TTCCAAACAT GGCTACAAGA TCTAAGAAAG CTAATAAAAG GGAAAAGTGG 
GTTTATGAGG ATGCAGCCAG AAATTCTTCG AATATGATAT TACACAATTT ATCCAATGCA 
CCAATTATCA CTGTTGATGC CTTAAGAGAT GAAACTTTAG AAATAATCTC AATTACTTCT 
TTGGCTCAGA AGGAAGAATG GCTGAGAAAA GTTTTTGGGT CAGGTAATAA CTCGACTCCT 
AGAAGCTTTG CCACGAGCAT TTATAATAAC CTCTCCAAAG AGGCTCATAA AGGGAATAGG 
TGGGCAATAT TATACTGCCA CAAAACCGGA AAATCATCTG TTATCGATTT ACAGAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_010569.3
CDS	1..4980
Translation	MSLSHLTLDQYYEIQCNELEAIRSIYMDDFTDLTKRKSSWDKQPQIIFEITLRSVDKEPVESSITLHFAMTPMYPYTAPEIEFKNVQNVMDSQLQMLKSEFKKIHNTSRGQEIIFEITSFTQEKLDEFQNVVNTQSLEDDRLQRIKETKEQLEKEEREKQQETIKKRSDEQRRIDEIVQRELEKRQDDDDDLLFNRTTQLDLQPPSEWVASGEAIVFSKTIKAKLPNNSMFKFKAVVNPKPIKLTSDIFSFSKQFLVKPYIPPESPLADFLMSSEMMENFYYLLSEIELDNSYFNTSNGKKEIANLEKELETVLKAKHDNVNRLFGYTVERMGRNNATFVWKIRLLTEYCNYYPLGDLIQSVGFVNLATARIWMIRLLEGLEAIHKLGIVHKCINLETVILVKDADFGSTIPKLVHSTYGYTVLNMLSRYPNKNGSSVELSPSTWIAPELLKFNNAKPQRLTDIWQLGVLFIQIISGSDIVMNFETPQEFLDSTSMDETLYDLLSKMLNNDPKKRLGTLELLPMKFLRTNIDSTINRFNLVSESVNSNSLELTPGDTITVRGNGGRTLSQSSIRRRSFNVGSRFSSINPATRSRYASDFEEIAVLGQGAFGQVVKARNALDSRYYAIKKIRHTEEKLSTILSEVMLLASLNHQYVVRYYAAWLEEDSMDENVFESTDEESDLSESSSDFEENDLLDQSSIFKNRTNHDLDNSNWDFISGSGYPDIVFENSSRDDENEDLDHDTSSTSSSESQDDTDKESKSIQNVPRRRNFVKPMTAVKKKSTLFIQMEYCENRTLYDLIHSENLNQQRDEYWRLFRQILEALSYIHSQGIIHRDLKPMNIFIDESRNVKIGDFGLAKNVHRSLDILKLDSQNLPGSSDNLTSAIGTAMYVATEVLDGTGHYNEKIDMYSLGIIFFEMIYPFSTGMERVNILKKLRSVSIEFPPDFDDNKMKVEKKIIRLLIDHDPNKRPGARTLLNSGWLPVKHQDEVIKEALKSLSNPSSPWQQQVRESLFNQSYSLTNDILFDNSVPTSTPFANILRSQMTEEVVKIFRKHGGIENNAPPRIFPKAPIYGTQNVYEVLDKGGTVLQLQYDLTYPMARYLSKNPSLISKQYRMQHVYRPPDHSRSSLEPRKFGEIDFDIISKSSSESGFYDAESLKIIDEILTVFPVFEKTNTFFILNHADILESVFNFTNIDKAQRPLVSRMLSQVGFARSFKEVKNELKAQLNISSTALNDLELFDFRLDFEAAKKRLYKLMIDSPHLKKIEDSLSHISKVLSYLKPLEVARNVVISPLSNYNSAFYKGGIMFHAVYDDGSSRNMIAAGGRYDTLISFFARPSGKKSSNTRKAVGFNLAWETIFGIAQNYFKLASGNRIKKRNRFLKDTAVDWKPSRCDVLISSFSNSLLDTIGVTILNTLWKQNIKADMLRDCSSVDDVVTGAQQDGIDWILLIKQQAYPLTNHKRKYKPLKIKKLSTNVDIDLDLDEFLTLYQQETGNKSLINDSLTLGDKADEFKRWDENSSAGSSQEGDIDDVVAGSTNNQKVIYVPNMATRSKKANKREKWVYEDAARNSSNMILHNLSNAPIITVDALRDETLEIISITSLAQKEEWLRKVFGSGNNSTPRSFATSIYNNLSKEAHKGNRWAILYCHKTGKSSVIDLQR

Target ORF information:

RefSeq Version	NM_001180591.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C serine/threonine-protein kinase GCN2 (GCN2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001180591.3

ORF Insert Sequence:

ATGTCATTGA GTCATCTCAC TTTAGATCAA TATTATGAAA TACAGTGCAA CGAACTTGAA 
GCGATACGTT CCATTTATAT GGATGACTTT ACTGACTTAA CTAAAAGAAA GTCTAGCTGG 
GATAAGCAGC CACAGATTAT ATTCGAAATT ACGCTTCGAT CTGTTGACAA AGAGCCGGTT 
GAATCTTCTA TAACATTACA TTTTGCGATG ACCCCAATGT ATCCTTATAC CGCTCCAGAA 
ATAGAATTCA AAAATGTACA AAATGTAATG GATAGTCAAT TGCAAATGCT GAAAAGTGAA 
TTTAAGAAAA TCCACAACAC CTCCCGAGGC CAAGAGATTA TATTTGAAAT TACATCTTTT 
ACTCAAGAAA AACTGGACGA ATTTCAAAAT GTGGTAAATA CACAGTCCTT GGAAGATGAT 
CGATTACAAA GAATCAAAGA AACTAAAGAA CAATTAGAAA AGGAAGAAAG AGAAAAACAA 
CAGGAAACAA TAAAAAAAAG ATCAGATGAG CAGCGAAGGA TAGATGAAAT TGTTCAAAGA 
GAGTTGGAGA AAAGACAAGA TGATGATGAT GATTTGCTAT TCAACAGAAC AACCCAGTTA 
GATTTACAAC CACCTTCAGA ATGGGTTGCA TCAGGTGAAG CTATTGTCTT TTCAAAAACT 
ATAAAGGCAA AATTGCCTAA TAACTCAATG TTCAAGTTTA AAGCAGTTGT AAATCCTAAG 
CCAATAAAAC TGACATCAGA TATATTTAGT TTTTCCAAAC AATTTCTTGT GAAGCCTTAT 
ATACCACCAG AATCTCCGTT GGCAGATTTT TTAATGTCTT CTGAAATGAT GGAAAATTTC 
TACTATTTGC TATCTGAAAT TGAATTGGAT AATAGCTATT TCAACACAAG TAATGGAAAA 
AAAGAAATAG CAAATTTAGA GAAAGAGTTA GAGACGGTGT TGAAAGCTAA GCATGACAAT 
GTGAATCGAT TGTTCGGTTA TACGGTGGAG CGCATGGGGA GAAATAATGC AACGTTTGTT 
TGGAAAATAA GACTCTTGAC AGAGTACTGT AACTACTATC CATTGGGAGA TTTGATACAA 
TCTGTTGGAT TTGTTAACTT AGCAACAGCG CGTATTTGGA TGATTAGATT GCTTGAAGGA 
TTGGAGGCCA TACACAAATT GGGAATTGTT CATAAATGTA TCAACTTAGA AACCGTGATC 
CTGGTGAAGG ATGCAGATTT TGGAAGCACT ATTCCCAAGT TAGTTCACTC CACTTATGGC 
TACACTGTTT TGAATATGCT ATCGAGATAT CCAAATAAAA ATGGTTCTTC GGTTGAGTTA 
TCTCCAAGTA CATGGATAGC CCCTGAGTTG TTGAAATTCA ATAACGCCAA ACCTCAAAGA 
TTAACTGATA TTTGGCAACT TGGTGTTTTG TTTATCCAGA TAATCAGTGG ATCTGATATA 
GTGATGAATT TTGAAACGCC TCAAGAATTC CTAGATTCAA CAAGTATGGA TGAAACTTTA 
TATGATCTTC TTTCGAAAAT GCTTAATAAC GATCCGAAGA AAAGATTAGG AACATTAGAA 
CTACTGCCCA TGAAATTCTT AAGGACCAAT ATTGACTCTA CAATCAATCG ATTTAACTTA 
GTTTCCGAAA GTGTCAATTC TAATTCCTTG GAGTTAACTC CTGGAGATAC CATAACCGTT 
CGGGGCAATG GAGGTAGAAC ACTTTCACAA TCGAGTATAC GAAGAAGATC ATTTAATGTT 
GGTTCCAGAT TCTCTTCTAT AAATCCTGCA ACGCGATCAC GATATGCTTC TGACTTTGAA 
GAGATTGCAG TTTTAGGCCA GGGCGCATTT GGACAAGTTG TCAAGGCACG TAATGCTCTC 
GATAGCAGAT ACTATGCGAT CAAGAAGATT AGACATACAG AAGAAAAGTT ATCTACTATA 
TTGAGTGAAG TAATGCTGTT AGCAAGCTTA AATCATCAAT ATGTTGTGCG TTACTATGCT 
GCATGGTTAG AAGAAGACAG TATGGATGAA AACGTTTTTG AATCAACTGA TGAAGAAAGT 
GACTTGAGCG AATCTTCCTC TGATTTTGAG GAAAATGATT TATTAGATCA AAGCAGTATT 
TTTAAAAATA GAACAAATCA CGATTTGGAT AATAGTAACT GGGATTTCAT ATCGGGGTCA 
GGATATCCGG ATATTGTCTT TGAAAATAGT TCTCGTGATG ATGAAAATGA AGATCTAGAC 
CATGATACTT CCTCGACTTC CTCGAGCGAA AGTCAAGATG ATACTGATAA AGAATCAAAG 
AGTATCCAGA ACGTTCCAAG AAGGAGGAAT TTTGTAAAAC CGATGACTGC TGTTAAGAAG 
AAAAGTACGC TTTTTATTCA AATGGAGTAC TGTGAAAATA GAACGCTATA TGATTTGATC 
CATTCTGAAA ATTTAAATCA ACAACGTGAT GAATATTGGA GGTTATTTCG ACAAATTTTG 
GAAGCACTGA GTTATATACA TTCCCAGGGT ATCATTCATA GGGATCTGAA GCCAATGAAT 
ATTTTTATAG ATGAATCGAG AAATGTTAAA ATCGGTGATT TTGGGTTAGC TAAGAACGTC 
CATAGATCTC TGGATATACT TAAGCTAGAT TCACAGAATT TGCCAGGCAG CTCAGATAAT 
TTAACATCCG CCATTGGTAC AGCAATGTAT GTTGCTACTG AAGTTTTAGA TGGTACAGGT 
CACTATAATG AAAAGATTGA TATGTATTCA CTTGGAATCA TTTTTTTTGA AATGATCTAT 
CCTTTCAGTA CAGGTATGGA GAGAGTTAAT ATTTTGAAAA AGTTACGATC AGTGTCGATA 
GAATTTCCTC CTGATTTCGA CGATAATAAG ATGAAAGTTG AAAAGAAAAT TATAAGGTTA 
CTCATAGACC ATGATCCCAA TAAAAGGCCT GGTGCTAGGA CATTATTAAA TAGTGGTTGG 
CTTCCTGTGA AGCATCAGGA TGAAGTAATC AAAGAGGCTT TAAAAAGTTT GTCGAATCCT 
TCATCCCCTT GGCAACAGCA AGTTCGAGAA AGTTTATTTA ACCAATCTTA CAGTCTAACA 
AATGATATTC TATTTGATAA CTCAGTTCCA ACATCCACTC CTTTCGCAAA CATTCTCAGG 
TCCCAAATGA CAGAAGAGGT AGTTAAAATT TTCAGGAAAC ATGGAGGAAT TGAAAATAAT 
GCTCCTCCGA GGATTTTTCC AAAGGCCCCC ATATACGGTA CGCAGAATGT ATATGAAGTG 
CTTGACAAGG GCGGTACCGT CTTGCAGTTA CAATATGATT TAACTTATCC TATGGCTAGG 
TATCTATCTA AAAATCCAAG TCTGATTTCT AAGCAATATA GGATGCAGCA CGTTTACCGA 
CCTCCTGATC ATTCAAGGTC AAGTTTGGAA CCTAGAAAGT TTGGTGAGAT TGACTTCGAC 
ATAATTTCAA AATCTTCCTC AGAGTCAGGA TTTTATGATG CAGAAAGCTT GAAAATTATC 
GATGAAATAT TAACCGTATT TCCTGTATTT GAGAAAACAA ACACTTTTTT CATATTAAAT 
CATGCTGATA TTTTGGAGAG TGTTTTCAAC TTTACAAATA TTGATAAAGC CCAAAGGCCT 
CTAGTTTCAC GAATGTTGTC GCAAGTAGGC TTTGCAAGGT CCTTCAAGGA AGTAAAGAAT 
GAACTAAAGG CGCAACTGAA CATATCTTCT ACGGCATTGA ATGATTTGGA GTTATTTGAT 
TTTAGACTGG ACTTCGAAGC AGCCAAAAAA CGCCTGTATA AATTGATGAT TGATAGTCCG 
CATCTAAAAA AAATTGAGGA CTCTTTGTCC CATATATCAA AGGTTCTCAG TTACCTAAAA 
CCCTTAGAAG TTGCAAGAAA TGTTGTGATA TCTCCTTTGA GTAACTACAA TAGCGCTTTT 
TACAAAGGAG GTATCATGTT TCATGCAGTT TATGACGATG GATCCTCACG TAATATGATA 
GCTGCTGGAG GGAGGTATGA CACTTTGATA TCCTTTTTTG CCAGACCATC AGGAAAAAAG 
AGCAGCAATA CTCGTAAGGC TGTAGGTTTC AACTTAGCGT GGGAAACAAT ATTCGGTATA 
GCCCAAAACT ATTTCAAACT CGCTTCTGGA AATAGGATAA AGAAGAGAAA TAGGTTTTTG 
AAAGATACAG CTGTTGATTG GAAGCCAAGC AGGTGTGATG TATTGATATC GAGTTTTTCG 
AACTCTTTGT TGGACACAAT CGGGGTTACA ATACTGAATA CATTGTGGAA GCAAAACATT 
AAAGCGGATA TGTTAAGGGA TTGTTCCTCG GTGGATGATG TCGTTACTGG CGCTCAACAG 
GATGGTATAG ACTGGATTTT GCTGATTAAG CAACAAGCGT ATCCACTAAC CAATCACAAG 
AGAAAGTACA AGCCATTAAA AATAAAAAAA TTGAGCACTA ATGTTGACAT AGATTTAGAT 
CTTGATGAGT TTTTAACCTT GTACCAACAA GAAACTGGTA ATAAATCTTT GATCAACGAT 
AGTCTCACTT TGGGCGATAA GGCTGATGAA TTTAAAAGAT GGGATGAAAA CAGCAGTGCC 
GGTAGTAGTC AAGAAGGTGA CATAGATGAT GTTGTTGCTG GTTCGACTAA TAATCAAAAG 
GTAATTTATG TTCCAAACAT GGCTACAAGA TCTAAGAAAG CTAATAAAAG GGAAAAGTGG 
GTTTATGAGG ATGCAGCCAG AAATTCTTCG AATATGATAT TACACAATTT ATCCAATGCA 
CCAATTATCA CTGTTGATGC CTTAAGAGAT GAAACTTTAG AAATAATCTC AATTACTTCT 
TTGGCTCAGA AGGAAGAATG GCTGAGAAAA GTTTTTGGGT CAGGTAATAA CTCGACTCCT 
AGAAGCTTTG CCACGAGCAT TTATAATAAC CTCTCCAAAG AGGCTCATAA AGGGAATAGG 
TGGGCAATAT TATACTGCCA CAAAACCGGA AAATCATCTG TTATCGATTT ACAGAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.
Nature387(6632 Suppl)75-8(1997 May)
Jacq C,Alt-M?rbe J,Andre B,Arnold W,Bahr A,Ballesta JP,Bargues M,Baron L,Becker A,Biteau N,Bl?cker H,Blugeon C,Boskovic J,Brandt P,Br?ckner M,Buitrago MJ,Coster F,Delaveau T,del Rey F,Dujon B,Eide LG,Garcia-Cantalejo JM,Goffeau A,Gomez-Peris A,Zaccaria P

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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GCN2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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