SKI2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SKI2
Entrez Gene ID	851114
Full Name	SKI complex RNA helicase subunit SKI2
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182286.3	NP_013502.3	SKI complex RNA helicase subunit SKI2

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003182	XP_312880.4
Homo sapiens (human)	SKIV2L	NP_008860.4
Danio rerio (zebrafish)	skiv2l	NP_001038366.1
Schizosaccharomyces pombe (fission yeast)	SPCC550.03c	NP_588095.1
Canis lupus familiaris (dog)	SKIV2L	XP_538841.2
Bos taurus (cattle)	SKIV2L	XP_003587743.1
Arabidopsis thaliana (thale cress)	AT3G46960	NP_190280.5
Pan troglodytes (chimpanzee)	SKIV2L	XP_001160927.2
	SKIV2L	XP_001106475.2
Rattus norvegicus (Norway rat)	Skiv2l	NP_998724.1
Drosophila melanogaster (fruit fly)	tst	NP_524465.1
Caenorhabditis elegans (roundworm)	skih-2	NP_502084.2
Saccharomyces cerevisiae (baker's yeast)	SKI2	NP_013502.3
Xenopus tropicalis (tropical clawed frog)	skiv2l	XP_004917184.1
Mus musculus (house mouse)	Skiv2l	NP_067312.2
Eremothecium gossypii	AGOS_AER067C	NP_984927.2
Neurospora crassa	NCU01857	XP_965488.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ski2, Ski3, and Ski8 assemble in a tetramer with 1:1:2 stoichiometry; 370 kDa core complex shows that Ski3 forms an array of 33 tetratricopeptide repeat motifs organized in N-terminal and C-terminal arms. The C-terminal arm of Ski3 and the two Ski8 subunits position the helicase core of Ski2 centrally within the complex, enhancing RNA binding.
Title: The yeast ski complex: crystal structure and RNA channeling to the exosome complex.

the 2.4 A resolution crystal structure of the 113 kDa helicase region of Saccharomyces cerevisiae Ski2 was determined.
Title: The crystal structure of S. cerevisiae Ski2, a DExH helicase associated with the cytoplasmic functions of the exosome.

Inactivating the Ski complex by mutating conserved residues in the DEVH helicase motif of Ski2 did not abrogate its interaction with Ski7p, indicating that Ski2p function is not necessary for this interaction.
Title: Domain interactions within the Ski2/3/8 complex and between the Ski complex and Ski7p.

The following SKI2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SKI2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00713	NM_001182286.3 Latest version!	Saccharomyces cerevisiae S288C SKI complex RNA helicase subunit SKI2 (SKI2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SKI2 ( NM_001182286.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013502.3 Saccharomyces cerevisiae S288C SKI complex RNA helicase subunit SKI2 (SKI2), partial mRNA.

Price & Availability↑

CloneID	OSi00713
Clone ID Related Accession (Same CDS sequence)	NM_001182286.3
Accession Version	NM_001182286.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	SKI complex RNA helicase subunit SKI2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). On Jul 30, 2012 this sequence version replaced NM_001182286.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTGAGG GATTCAGTAG CAGTTCCATA CAGGAATTAT ATCAATCGTT AAAAGAAATC 
ACTAACAACG CAGACGTAGA GCTTTTCGAA GATCGCATTA CTAAATTAGA TTTTGAGAGC 
ACCGATGAAC CAAAGCATGC AAATGATATC ATAAAGGATA GATTTTTGAG ACCTTCTAAT 
GCTCTTCCTT GGAGCTTGTT GGACATGGTC CAAGACGTTC CACATACGTC TTCTCCAGAA 
GATTGCTCTG GTAAACTAGA TTACAAGGAA TTGCTGAAAG TCCCGGACCC AATTAATAGA 
ACATCATACC AGTTTAAAAG AACTGGGTTA GAAGGTAAAA TTTCAGGCTA CAAAGAAGAG 
GTTGATTTGA AGGAAGTTGC AAATGCCAAT GCATCAAATT CACTGTCGAT TACGAGAAGT 
ATCAACCATA ACCAAAACTC AGTAAGGGGT TCTACAGCGC AATTGCCTTT CACACCAGGC 
GGAATTCCCA TGAAATCTGT CAAGACAGAT TCAGAACAAA ATGGATCTTC TACAATGGCC 
AACGCTACTA AATTGTTACA CAAGGATGGT CAAGGTTTAT TCGATATTCC AGAGGGTATG 
AATAGAGGCA TTAAGCCGAT GGATTCTCCA GCTGAAAATG AGGATCAAAA CGGACAGTTC 
AAAGAATTGA AACAGCTAAA TGAGATAGAT AATGAATTAG ACATAAGGAT TGAGGCGAAC 
GAGGCAAAGT TAAAAGAGGA GGAAAAATCT GCCAAGTCAA TTTCTGAAGA AATTATGGAG 
GAAGCCACTG AAGAAACGAC TGCCGATAAT GCTGATGATG CCGAAATCGA TGAACTATTA 
CCAATTGGAA TAGACTTTGG CAGAACTAAA CCAGTTTCGA AAAGTGTTCC TGTTAAAAAG 
GAATGGGCCC ATGTTGTTGA TTTGAACCAT AAGATCGAAA ATTTCGATGA GTTGATTCCA 
AACCCCGCTA GATCGTGGCC TTTTGAATTG GATACATTTC AAAAGGAAGC CGTCTATCAT 
CTCGAACAAG GTGATTCCGT ATTTGTTGCC GCCCACACAT CTGCTGGTAA AACTGTTGTT 
GCAGAATACG CAATTGCCAT GGCACATAGA AACATGACTA AAACTATATA TACATCACCC 
ATTAAAGCCT TATCCAATCA AAAATTCCGA GACTTTAAAG AAACTTTTGA CGATGTTAAT 
ATTGGGTTGA TTACTGGTGA TGTGCAAATT AATCCGGATG CTAACTGTTT GATTATGACG 
ACCGAAATTT TAAGATCAAT GCTTTATAGA GGTGCCGATT TGATCAGAGA TGTAGAGTTT 
GTCATTTTCG ATGAAGTTCA CTACGTTAAT GATCAAGACC GTGGTGTTGT CTGGGAAGAA 
GTTATTATTA TGCTTCCACA GCATGTCAAG TTTATCTTAT TATCTGCCAC CGTTCCTAAC 
ACTTATGAGT TTGCTAATTG GATTGGAAGA ACTAAGCAAA AAAATATTTA TGTCATTTCC 
ACACCAAAAA GACCCGTTCC ATTGGAAATA AATATATGGG CTAAAAAGGA ACTGATACCG 
GTTATTAATC AAAATTCAGA ATTTTTAGAA GCTAATTTCA GGAAACATAA AGAGATTCTA 
AATGGGGAGA GTGCAAAAGG GGCTCCTTCA AAAACTGACA ACGGGAGAGG TGGATCCACA 
GCAAGAGGGG GCCGTGGTGG ATCCAATACT CGAGATGGAA GAGGAGGCCG TGGTAATTCA 
ACACGTGGAG GTGCCAACCG TGGAGGTTCA AGAGGTGCTG GCGCAATTGG CTCCAACAAG 
CGTAAGTTTT TCACTCAAGA TGGTCCTTCG AAAAAAACAT GGCCCGAAAT TGTCAATTAC 
CTAAGGAAAA GAGAGCTTCT ACCAATGGTT GTTTTCGTAT TTAGTAAAAA GAGGTGCGAG 
GAATATGCTG ACTGGTTGGA GGGAATTAAC TTCTGCAACA ATAAGGAAAA ATCACAAATC 
CACATGTTTA TTGAGAAGTC GATTACTCGT TTGAAAAAAG AGGATAGAGA TTTGCCTCAA 
ATTCTTAAAA CCAGATCATT ACTTGAACGT GGTATAGCTG TTCATCATGG GGGGCTATTG 
CCCATTGTCA AGGAGTTGAT CGAAATATTA TTTTCCAAGG GTTTTATCAA AGTTTTATTT 
GCAACAGAAA CTTTTGCCAT GGGTTTGAAC CTTCCCACAA GAACAGTTAT CTTTAGCAGC 
ATCCGTAAAC ATGATGGTAA TGGATTAAGA GAACTGACTC CAGGTGAGTT TACCCAAATG 
GCTGGTAGAG CTGGTAGAAG GGGCTTGGAT TCCACTGGTA CAGTCATTGT GATGGCATAT 
AATAGTCCTT TATCTATTGC TACGTTCAAA GAAGTAACGA TGGGTGTTCC GACGAGGTTG 
CAGTCTCAGT TTAGATTAAC ATACAATATG ATATTAAATC TTTTAAGAAT TGAGGCGTTG 
AGAGTTGAAG AAATGATCAA GTATTCTTTT AGTGAGAATG CCAAAGAAAC TTTACAACCT 
GAACATGAAA AACAAATCAA AGTATTACAA GAGGAATTAC AAACCATAGA GTACAAAAGT 
TGTGAAATCT GTGATAATGA TATCGAAAAG TTTTTGGAAT TGATGCTGGC ATATAAGGAG 
GCAACAGTCA ACCTAATGCA AGAAATGGTT AAATCGCCTT CGATATTGCA TATCTTGAAA 
GAGGGCAGAC TCGTTGCTTT TAGGGACCCC AATGATTGCT TGAAATTAGG ATTTGTATTT 
AAAGTTTCTC TGAAGGATGC TGTTTGTGTC ATTATGACGT TCACAAAACC ATACAAACTC 
CCAAATGGTG AGCCAAATCA TTTAATATAC TTTCCAAAAG CGGATGGATA CAGAAGAAGG 
AACTTCCCTA AATTCCAGAA GACTGATTTT TATATGGAAG AAGTTCCTGT GACGGCTATT 
GAAGTGATCA CTAAACGCAA GTTTGCCGCA CCCTTGGGAA AAGTGATTAA AAAGGATGTT 
GCCGCTTTGA ACGAGTTTAA CGCTGAAACA AATAACATTT TGGACGGCAA AACGCTAAAA 
GAAGCAATTA ACATTGAAAA GCAAGGTTTA AAAATTCACC AAATATTGTT AGACCGTACA 
AATATAAGGG ATGAGATATT CAAACTAAAA AGCATTAAAT GTCCCAACCT AAGCCAACAC 
ATTGTTCCCA AGTTTAAGGC GCATGTAATT AAAAAGAAAA TTGAAGAATT GTACCATTTG 
ATGTCTGACC AAAATCTGAG CTTACTACCC GATTATGAAA AGAGATTGGC TGTTCTGAAA 
GACACTGAAT TTATCGACCA AAACCACAAC GTGCTGTTAA AAGGTAGAGT GGCATGTGAA 
ATTAATTCTG GTTATGAGTT AGTTTTAACT GAATTAATTT TAGACAACTT TTTAGGAAGC 
TTTGAACCTG AAGAGATTGT AGCCTTATTA TCAGTATTTG TTTATGAGGG GAAAACCAGG 
GAAGAGGAGC CTCCTATTGT CACACCAAGG CTAGCAAAGG GTAAACAAAG AATTGAGGAA 
ATTTACAAGA AAATGCTTTG TGTTTTCAAT ACGCATCAGA TTCCATTAAC TCAGGATGAA 
GCTGAGTTTT TGGACAGAAA AAGGTTTGCC ATGATGAATG TGGTGTATGA GTGGGCCCGT 
GGTTTATCAT TCAAAGAAAT CATGGAAATG AGCCCCGAGG CTGAAGGTAC TGTAGTCAGA 
GTTATCACTT GGCTAGACGA AATTTGCCGC GAAGTTAAGA CTGCCTCTAT TATTATTGGT 
AATTCCACAT TACATATGAA GATGAGCAGG GCTCAAGAGT TGATTAAGAG AGATATTGTT 
TTCGCCGCAA GTTTGTATTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013502.3
CDS	1..3864
Translation	MSEGFSSSSIQELYQSLKEITNNADVELFEDRITKLDFESTDEPKHANDIIKDRFLRPSNALPWSLLDMVQDVPHTSSPEDCSGKLDYKELLKVPDPINRTSYQFKRTGLEGKISGYKEEVDLKEVANANASNSLSITRSINHNQNSVRGSTAQLPFTPGGIPMKSVKTDSEQNGSSTMANATKLLHKDGQGLFDIPEGMNRGIKPMDSPAENEDQNGQFKELKQLNEIDNELDIRIEANEAKLKEEEKSAKSISEEIMEEATEETTADNADDAEIDELLPIGIDFGRTKPVSKSVPVKKEWAHVVDLNHKIENFDELIPNPARSWPFELDTFQKEAVYHLEQGDSVFVAAHTSAGKTVVAEYAIAMAHRNMTKTIYTSPIKALSNQKFRDFKETFDDVNIGLITGDVQINPDANCLIMTTEILRSMLYRGADLIRDVEFVIFDEVHYVNDQDRGVVWEEVIIMLPQHVKFILLSATVPNTYEFANWIGRTKQKNIYVISTPKRPVPLEINIWAKKELIPVINQNSEFLEANFRKHKEILNGESAKGAPSKTDNGRGGSTARGGRGGSNTRDGRGGRGNSTRGGANRGGSRGAGAIGSNKRKFFTQDGPSKKTWPEIVNYLRKRELLPMVVFVFSKKRCEEYADWLEGINFCNNKEKSQIHMFIEKSITRLKKEDRDLPQILKTRSLLERGIAVHHGGLLPIVKELIEILFSKGFIKVLFATETFAMGLNLPTRTVIFSSIRKHDGNGLRELTPGEFTQMAGRAGRRGLDSTGTVIVMAYNSPLSIATFKEVTMGVPTRLQSQFRLTYNMILNLLRIEALRVEEMIKYSFSENAKETLQPEHEKQIKVLQEELQTIEYKSCEICDNDIEKFLELMLAYKEATVNLMQEMVKSPSILHILKEGRLVAFRDPNDCLKLGFVFKVSLKDAVCVIMTFTKPYKLPNGEPNHLIYFPKADGYRRRNFPKFQKTDFYMEEVPVTAIEVITKRKFAAPLGKVIKKDVAALNEFNAETNNILDGKTLKEAINIEKQGLKIHQILLDRTNIRDEIFKLKSIKCPNLSQHIVPKFKAHVIKKKIEELYHLMSDQNLSLLPDYEKRLAVLKDTEFIDQNHNVLLKGRVACEINSGYELVLTELILDNFLGSFEPEEIVALLSVFVYEGKTREEEPPIVTPRLAKGKQRIEEIYKKMLCVFNTHQIPLTQDEAEFLDRKRFAMMNVVYEWARGLSFKEIMEMSPEAEGTVVRVITWLDEICREVKTASIIIGNSTLHMKMSRAQELIKRDIVFAASLYL

Target ORF information:

RefSeq Version	NM_001182286.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C SKI complex RNA helicase subunit SKI2 (SKI2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182286.3

ORF Insert Sequence:

ATGTCTGAGG GATTCAGTAG CAGTTCCATA CAGGAATTAT ATCAATCGTT AAAAGAAATC 
ACTAACAACG CAGACGTAGA GCTTTTCGAA GATCGCATTA CTAAATTAGA TTTTGAGAGC 
ACCGATGAAC CAAAGCATGC AAATGATATC ATAAAGGATA GATTTTTGAG ACCTTCTAAT 
GCTCTTCCTT GGAGCTTGTT GGACATGGTC CAAGACGTTC CACATACGTC TTCTCCAGAA 
GATTGCTCTG GTAAACTAGA TTACAAGGAA TTGCTGAAAG TCCCGGACCC AATTAATAGA 
ACATCATACC AGTTTAAAAG AACTGGGTTA GAAGGTAAAA TTTCAGGCTA CAAAGAAGAG 
GTTGATTTGA AGGAAGTTGC AAATGCCAAT GCATCAAATT CACTGTCGAT TACGAGAAGT 
ATCAACCATA ACCAAAACTC AGTAAGGGGT TCTACAGCGC AATTGCCTTT CACACCAGGC 
GGAATTCCCA TGAAATCTGT CAAGACAGAT TCAGAACAAA ATGGATCTTC TACAATGGCC 
AACGCTACTA AATTGTTACA CAAGGATGGT CAAGGTTTAT TCGATATTCC AGAGGGTATG 
AATAGAGGCA TTAAGCCGAT GGATTCTCCA GCTGAAAATG AGGATCAAAA CGGACAGTTC 
AAAGAATTGA AACAGCTAAA TGAGATAGAT AATGAATTAG ACATAAGGAT TGAGGCGAAC 
GAGGCAAAGT TAAAAGAGGA GGAAAAATCT GCCAAGTCAA TTTCTGAAGA AATTATGGAG 
GAAGCCACTG AAGAAACGAC TGCCGATAAT GCTGATGATG CCGAAATCGA TGAACTATTA 
CCAATTGGAA TAGACTTTGG CAGAACTAAA CCAGTTTCGA AAAGTGTTCC TGTTAAAAAG 
GAATGGGCCC ATGTTGTTGA TTTGAACCAT AAGATCGAAA ATTTCGATGA GTTGATTCCA 
AACCCCGCTA GATCGTGGCC TTTTGAATTG GATACATTTC AAAAGGAAGC CGTCTATCAT 
CTCGAACAAG GTGATTCCGT ATTTGTTGCC GCCCACACAT CTGCTGGTAA AACTGTTGTT 
GCAGAATACG CAATTGCCAT GGCACATAGA AACATGACTA AAACTATATA TACATCACCC 
ATTAAAGCCT TATCCAATCA AAAATTCCGA GACTTTAAAG AAACTTTTGA CGATGTTAAT 
ATTGGGTTGA TTACTGGTGA TGTGCAAATT AATCCGGATG CTAACTGTTT GATTATGACG 
ACCGAAATTT TAAGATCAAT GCTTTATAGA GGTGCCGATT TGATCAGAGA TGTAGAGTTT 
GTCATTTTCG ATGAAGTTCA CTACGTTAAT GATCAAGACC GTGGTGTTGT CTGGGAAGAA 
GTTATTATTA TGCTTCCACA GCATGTCAAG TTTATCTTAT TATCTGCCAC CGTTCCTAAC 
ACTTATGAGT TTGCTAATTG GATTGGAAGA ACTAAGCAAA AAAATATTTA TGTCATTTCC 
ACACCAAAAA GACCCGTTCC ATTGGAAATA AATATATGGG CTAAAAAGGA ACTGATACCG 
GTTATTAATC AAAATTCAGA ATTTTTAGAA GCTAATTTCA GGAAACATAA AGAGATTCTA 
AATGGGGAGA GTGCAAAAGG GGCTCCTTCA AAAACTGACA ACGGGAGAGG TGGATCCACA 
GCAAGAGGGG GCCGTGGTGG ATCCAATACT CGAGATGGAA GAGGAGGCCG TGGTAATTCA 
ACACGTGGAG GTGCCAACCG TGGAGGTTCA AGAGGTGCTG GCGCAATTGG CTCCAACAAG 
CGTAAGTTTT TCACTCAAGA TGGTCCTTCG AAAAAAACAT GGCCCGAAAT TGTCAATTAC 
CTAAGGAAAA GAGAGCTTCT ACCAATGGTT GTTTTCGTAT TTAGTAAAAA GAGGTGCGAG 
GAATATGCTG ACTGGTTGGA GGGAATTAAC TTCTGCAACA ATAAGGAAAA ATCACAAATC 
CACATGTTTA TTGAGAAGTC GATTACTCGT TTGAAAAAAG AGGATAGAGA TTTGCCTCAA 
ATTCTTAAAA CCAGATCATT ACTTGAACGT GGTATAGCTG TTCATCATGG GGGGCTATTG 
CCCATTGTCA AGGAGTTGAT CGAAATATTA TTTTCCAAGG GTTTTATCAA AGTTTTATTT 
GCAACAGAAA CTTTTGCCAT GGGTTTGAAC CTTCCCACAA GAACAGTTAT CTTTAGCAGC 
ATCCGTAAAC ATGATGGTAA TGGATTAAGA GAACTGACTC CAGGTGAGTT TACCCAAATG 
GCTGGTAGAG CTGGTAGAAG GGGCTTGGAT TCCACTGGTA CAGTCATTGT GATGGCATAT 
AATAGTCCTT TATCTATTGC TACGTTCAAA GAAGTAACGA TGGGTGTTCC GACGAGGTTG 
CAGTCTCAGT TTAGATTAAC ATACAATATG ATATTAAATC TTTTAAGAAT TGAGGCGTTG 
AGAGTTGAAG AAATGATCAA GTATTCTTTT AGTGAGAATG CCAAAGAAAC TTTACAACCT 
GAACATGAAA AACAAATCAA AGTATTACAA GAGGAATTAC AAACCATAGA GTACAAAAGT 
TGTGAAATCT GTGATAATGA TATCGAAAAG TTTTTGGAAT TGATGCTGGC ATATAAGGAG 
GCAACAGTCA ACCTAATGCA AGAAATGGTT AAATCGCCTT CGATATTGCA TATCTTGAAA 
GAGGGCAGAC TCGTTGCTTT TAGGGACCCC AATGATTGCT TGAAATTAGG ATTTGTATTT 
AAAGTTTCTC TGAAGGATGC TGTTTGTGTC ATTATGACGT TCACAAAACC ATACAAACTC 
CCAAATGGTG AGCCAAATCA TTTAATATAC TTTCCAAAAG CGGATGGATA CAGAAGAAGG 
AACTTCCCTA AATTCCAGAA GACTGATTTT TATATGGAAG AAGTTCCTGT GACGGCTATT 
GAAGTGATCA CTAAACGCAA GTTTGCCGCA CCCTTGGGAA AAGTGATTAA AAAGGATGTT 
GCCGCTTTGA ACGAGTTTAA CGCTGAAACA AATAACATTT TGGACGGCAA AACGCTAAAA 
GAAGCAATTA ACATTGAAAA GCAAGGTTTA AAAATTCACC AAATATTGTT AGACCGTACA 
AATATAAGGG ATGAGATATT CAAACTAAAA AGCATTAAAT GTCCCAACCT AAGCCAACAC 
ATTGTTCCCA AGTTTAAGGC GCATGTAATT AAAAAGAAAA TTGAAGAATT GTACCATTTG 
ATGTCTGACC AAAATCTGAG CTTACTACCC GATTATGAAA AGAGATTGGC TGTTCTGAAA 
GACACTGAAT TTATCGACCA AAACCACAAC GTGCTGTTAA AAGGTAGAGT GGCATGTGAA 
ATTAATTCTG GTTATGAGTT AGTTTTAACT GAATTAATTT TAGACAACTT TTTAGGAAGC 
TTTGAACCTG AAGAGATTGT AGCCTTATTA TCAGTATTTG TTTATGAGGG GAAAACCAGG 
GAAGAGGAGC CTCCTATTGT CACACCAAGG CTAGCAAAGG GTAAACAAAG AATTGAGGAA 
ATTTACAAGA AAATGCTTTG TGTTTTCAAT ACGCATCAGA TTCCATTAAC TCAGGATGAA 
GCTGAGTTTT TGGACAGAAA AAGGTTTGCC ATGATGAATG TGGTGTATGA GTGGGCCCGT 
GGTTTATCAT TCAAAGAAAT CATGGAAATG AGCCCCGAGG CTGAAGGTAC TGTAGTCAGA 
GTTATCACTT GGCTAGACGA AATTTGCCGC GAAGTTAAGA CTGCCTCTAT TATTATTGGT 
AATTCCACAT TACATATGAA GATGAGCAGG GCTCAAGAGT TGATTAAGAG AGATATTGTT 
TTCGCCGCAA GTTTGTATTT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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SKI2 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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