HAP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	HAP1
Entrez Gene ID	850958
Full Name	Hap1p
Synonyms	CYP1
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182143.1	NP_013357.1	Hap1p

Pathways from BioSystems

Homologies

Kluyveromyces lactis	KLLA0F22990g	XP_456105.1
Eremothecium gossypii	AGOS_AFR117C	NP_985664.2
Saccharomyces cerevisiae (baker's yeast)	HAP1	NP_013357.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Hap1 also plays a significant and direct role under hypoxic conditions, not as an activator, but as a repressor.
Title: Heme levels switch the function of Hap1 of Saccharomyces cerevisiae between transcriptional activator and transcriptional repressor.

analysis of a complex mechanism of transcriptional regulation at the HAP1 promoter, involving multiple DNA elements and regulatory proteins
Title: Regulation of the HAP1 gene involves positive actions of histone deacetylases.

Hap1 binds to DNA and activates transcription of genes encoding functions required for respiration and for controlling oxidative damage.
Title: The heme activator protein Hap1 represses transcription by a heme-independent mechanism in Saccharomyces cerevisiae.

A simultaneous optimization of distinct intermolecular interactions determines the DNA-binding specificity of Hap1. Perturbations to individual interactions modulate Hap1 binding affinity and, very likely, its binding geometry.
Title: Control of Hap1-DNA site recognition through the interplay of multiple distinct intermolecular interactions.

The following HAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00587	NM_001182143.1 Latest version!	Saccharomyces cerevisiae S288C Hap1p (HAP1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HAP1 ( NM_001182143.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013357.1 Saccharomyces cerevisiae S288C Hap1p (HAP1), partial mRNA.

Price & Availability↑

CloneID	OSi00587
Clone ID Related Accession (Same CDS sequence)	NM_001182143.1
Accession Version	NM_001182143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	Hap1p
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCGAATA CCCCTTATAA TTCATCTGTG CCTTCCATTG CATCCATGAC CCAGTCTTCG 
GTCTCAAGAA GTCCTAACAT GCATACAGCA ACTACGCCCG GTGCCAACAC CAGCTCTAAC 
TCTCCACCCT TGCACATGTC TTCAGATTCG TCCAAGATCA AGAGGAAGCG TAACAGAATT 
CCGCTCAGTT GCACCATTTG TCGGAAAAGG AAAGTCAAAT GTGACAAACT CAGACCACAC 
TGCCAGCAGT GCACTAAAAC TGGGGTAGCC CATCTCTGCC ACTACATGGA ACAGACCTGG 
GCAGAAGAGG CAGAGAAAGA ATTGCTGAAG GACAACGAAT TAAAGAAGCT TAGGGAGCGC 
GTAAAATCTT TAGAAAAGAC TCTTTCTAAG GTGCACTCTT CTCCTTCGTC TAACTCCTTG 
AAAAGTTACA ACACTCCCGA GAGCAGCAAC CTGTTTATGG GTAGCGATGA ACACACCACC 
CTTGTTAATG CAAATACAGG CTCTGCTTCC TCTGCCTCGC ATATGCATCA GCAACAACAG 
CAACAGCAGC AACAGGAACA ACAACAAGAC TTTTCCAGAA GTGCGAACGC CAACGCGAAT 
TCCTCGTCCC TTTCTATCTC AAATAAATAT GACAACGATG AGCTGGACTT AACTAAGGAC 
TTTGATCTTT TGCATATCAA AAGTAACGGA ACCATCCACT TAGGTGCCAC CCACTGGTTG 
TCTATCATGA AAGGTGACCC GTACCTAAAA CTTTTGTGGG GTCATATCTT CGCTATGAGG 
GAAAAGTTAA ATGAATGGTA CTACCAAAAA AATTCGTACT CTAAGCTGAA GTCAAGCAAA 
TGTCCCATCA ATCACGCGCA AGCGCCGCCT TCTGCCGCTG CCGCCGCTAC CAGAAAATGT 
CCTGTTGATC ACTCCGCGTT TTCGTCTGGC ATGGTGGCCC CAAAGGAGGA GACTCCTCTT 
CCTAGGAAAT GTCCAGTTGA CCACACCATG TTCTCTTCGG GAATGATTCC TCCCAGAGAG 
GACACTTCGT CCCAGAAGAG GTGTCCCGTT GACCACACCA TGTATTCCGC AGGAATGATG 
CCGCCCAAGG ACGAGACACC TTCCCCATTT TCCACTAAAG CTATGATAGA CCATAACAAG 
CATACAATGA ATCCGCCTCA GTCAAAATGT CCTGTGGACC ATAGAAACTA TATGAAGGAT 
TATCCCTCTG ACATGGCAAA TTCTTCTTCG AACCCGGCAA GTCGTTGCCC CATTGACCAT 
TCAAGCATGA AAAATACAGC GGCCTTACCA GCTTCAACGC ACAATACCAT CCCACACCAC 
CAACCACAGT CCGGATCTCA TGCTCGTTCG CATCCCGCAC AAAGCAGGAA ACATGATTCC 
TACATGACAG AATCTGAAGT CCTCGCAACA CTTTGTGAGA TGTTGCCACC AAAGCGCGTC 
ATCGCATTAT TCATCGAGAA ATTCTTCAAA CATTTATACC CTGCCATTCC AATCTTAGAT 
GAACAGAATT TCAAAAATCA CGTGAATCAA ATGCTTTCGT TGTCTTCGAT GAATCCCACA 
GTTAACAACT TTGGTATGAG CATGCCATCT TCATCTACAC TAGAGAACCA ACCCATAACA 
CAAATCAATC TTCCAAAACT TTCCGATTCT TGTAACTTAG GTATTCTGAT AATAATCTTG 
AGATTGACAT GGCTATCCAT ACCTTCTAAT TCCTGCGAAG TCGACCTGGG AGAAGAAAGT 
GGCTCATTTT TAGTGCCCAA CGAATCTAGC AATATGTCTG CATCTGCATT GACCTCGATG 
GCTAAAGAAG AATCACTTCT GCTAAAGCAT GAGACACCGG TCGAGGCACT GGAGCTATGT 
CAAAAATACT TGATTAAATT CGATGAACTT TCTAGTATTT CCAATAACAA CGTTAATTTA 
ACCACGGTGC AGTTTGCCAT TTTTTACAAC TTCTATATGA AAAGTGCCTC TAATGATTTG 
ACTACCTTGA CAAATACCAA CAACACTGGC ATGGCCAATC CTGGTCACGA TTCCGAGTCT 
CACCAGATCC TATTGTCCAA TATTACTCAA ATGGCCTTTA GTTGTGGGTT ACACAGAGAC 
CCTGATAATT TTCCTCAATT AAACGCTACC ATTCCAGCAA CCAGCCAGGA CGTGTCTAAC 
AACGGGAGCA AAAAGGCAAA CCCTAGCACC AATCCAACTT TGAATAACAA CATGTCTGCT 
GCCACTACCA ACAGCAGTAG CAGATCTGGC AGTGCTGATT CAAGAAGTGG TTCTAACCCT 
GTGAACAAGA AGGAAAATCA GGTTAGTATC GAAAGATTTA AACACACTTG GAGGAAAATT 
TGGTATTACA TTGTTAGCAT GGATGTTAAC CAATCTCTTT CCCTGGGGAG CCCTCGACTA 
CTAAGAAATC TGAGGGATTT CAGCGATACA AAGCTACCAA GTGCGTCAAG GATTGATTAT 
GTTCGCGATA TCAAAGAGTT AATCATTGTG AAGAATTTTA CTCTTTTTTT CCAAATTGAT 
TTGTGTATTA TTGCTGTATT AAATCACATT TTGAATGTTT CTTTAGCAAG AAGCGTGAGA 
AAATTTGAAC TGGATTCATT GATTAATTTA TTGAAAAATC TGACCTATGG TACTGAGAAT 
GTCAATGATG TAGTGAGCTC CTTGATCAAC AAAGGGTTAT TACCAACTTC GGAAGGTGGT 
TCTGTAGATT CAAATAATGA TGAAATTTAC GGTCTACCGA AACTACCCGA TATTCTAAAC 
CATGGTCAAC ATAACCAAAA CTTGTATGCT GATGGAAGAA ATACTTCTAG TAGTGATATA 
GATAAGAAAT TGGACCTTCC TCACGAATCT ACAACGAGAG CTCTATTCTT TTCCAAGCAT 
ATGACAATTA GAATGTTGCT GTACTTATTG AACTACATTT TGTTTACTCA TTATGAACCA 
ATGGGCAGTG AAGATCCTGG TACTAATATC CTAGCTAAGG AGTACGCTCA AGAGGCATTA 
AATTTTGCCA TGGATGGCTA CAGAAACTGC ATGATTTTCT TCAACAATAT CAGAAACACC 
AATTCACTAT TCGATTACAT GAATGTTATC TTGTCTTACC CTTGTTTGGA CATTGGACAT 
CGTTCTTTAC AATTTATCGT TTGTTTGATC CTGAGAGCTA AATGTGGCCC ATTGACTGGT 
ATGCGTGAAT CATCGATCAT TACTAATGGT ACATCAAGTG GATTTAATAG TTCGGTAGAA 
GATGAGGACG TCAAAGTTAA ACAAGAATCT TCTGATGAAT TGAAAAAAGA CGATTTCATG 
AAAGATGTAA ATTTGGATTC AGGCGATTCA TTAGCAGAGA TTCTAATGTC AAGAATGCTG 
CTATTTCAAA AACTAACAAA ACAACTATCA AAGAAGTACA ACTACGCTAT TCGTATGAAC 
AAATCCACTG GATTCTTTGT CTCTTTACTA GATACACCTT CAAAGAAATC AGACTCGAAA 
TCGGGTGGTA GTTCATTCAT GTTGGGTAAT TGGAAACATC CAAAGGTTTC AAACATGAGC 
GGATTTCTTG CTGGTGACAA AGACCAATTA CAGAAATGCC CCGTGTACCA AGATGCGCTG 
GGGTTTGTTA GTCCAACCGG TGCTAATGAA GGTTCTGCTC CGATGCAAGG CATGTCCTTA 
CAGGGCTCTA CTGCTAGGAT GGGAGGGACC CAGTTGCCAC CAATTAGATC ATACAAACCT 
ATCACGTACA CAAGTAGTAA TCTACGTCGT ATGAATGAAA CGGGTGAGGC AGAAGCTAAG 
AGAAGAAGAT TTAATGATGG CTATATTGAT AATAATAGTA ACAACGATAT ACCTAGAGGA 
ATCAGCCCAA AACCTTCAAA TGGGCTATCA TCGGTGCAGC CACTATTATC GTCATTTTCC 
ATGAACCAGC TAAACGGGGG TACCATTCCA ACGGTTCCAT CGTTAACCAA CATTACTTCA 
CAAATGGGAG CTTTACCATC TTTAGATAGG ATCACCACTA ATCAAATAAA TTTGCCAGAC 
CCATCTAGAG ATGAAGCATT TGACAACTCC ATCAAGCAAA TGACGCCTAT GACAAGTGCA 
TTCATGAATG CTAATACTAC AATTCCAAGT TCAACTTTAA ACGGGAATAT GAACATGAAT 
GGAGCTGGAA CTGCGAATAC AGATACAAGT GCCAACGGCA GTGCTTTATC GACACTGACA 
AGCCCACAAG GCTCAGACTT AGCATCCAAT TCTGCTACAC AGTATAAACC TGACTTAGAA 
GACTTTTTGA TGCAAAATTC TAACTTTAAT GGGCTAATGA TAAATCCTTC CAGTCTGGTA 
GAAGTTGTTG GTGGATACAA CGATCCTAAT AACCTTGGAA GAAATGACGC GGTTGATTTT 
CTACCCGTTG ATAATGTTGA AATTGATGGT GTTGGAATAA AAATCAACTA TCATCTACTA 
ACTAGTATTT ACGTTACTAG TATATTATCA TATACGGTGT TAGAAGATGA CGCAAATGAT 
GAGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013357.1
CDS	1..4509
Translation	MSNTPYNSSVPSIASMTQSSVSRSPNMHTATTPGANTSSNSPPLHMSSDSSKIKRKRNRIPLSCTICRKRKVKCDKLRPHCQQCTKTGVAHLCHYMEQTWAEEAEKELLKDNELKKLRERVKSLEKTLSKVHSSPSSNSLKSYNTPESSNLFMGSDEHTTLVNANTGSASSASHMHQQQQQQQQQEQQQDFSRSANANANSSSLSISNKYDNDELDLTKDFDLLHIKSNGTIHLGATHWLSIMKGDPYLKLLWGHIFAMREKLNEWYYQKNSYSKLKSSKCPINHAQAPPSAAAAATRKCPVDHSAFSSGMVAPKEETPLPRKCPVDHTMFSSGMIPPREDTSSQKRCPVDHTMYSAGMMPPKDETPSPFSTKAMIDHNKHTMNPPQSKCPVDHRNYMKDYPSDMANSSSNPASRCPIDHSSMKNTAALPASTHNTIPHHQPQSGSHARSHPAQSRKHDSYMTESEVLATLCEMLPPKRVIALFIEKFFKHLYPAIPILDEQNFKNHVNQMLSLSSMNPTVNNFGMSMPSSSTLENQPITQINLPKLSDSCNLGILIIILRLTWLSIPSNSCEVDLGEESGSFLVPNESSNMSASALTSMAKEESLLLKHETPVEALELCQKYLIKFDELSSISNNNVNLTTVQFAIFYNFYMKSASNDLTTLTNTNNTGMANPGHDSESHQILLSNITQMAFSCGLHRDPDNFPQLNATIPATSQDVSNNGSKKANPSTNPTLNNNMSAATTNSSSRSGSADSRSGSNPVNKKENQVSIERFKHTWRKIWYYIVSMDVNQSLSLGSPRLLRNLRDFSDTKLPSASRIDYVRDIKELIIVKNFTLFFQIDLCIIAVLNHILNVSLARSVRKFELDSLINLLKNLTYGTENVNDVVSSLINKGLLPTSEGGSVDSNNDEIYGLPKLPDILNHGQHNQNLYADGRNTSSSDIDKKLDLPHESTTRALFFSKHMTIRMLLYLLNYILFTHYEPMGSEDPGTNILAKEYAQEALNFAMDGYRNCMIFFNNIRNTNSLFDYMNVILSYPCLDIGHRSLQFIVCLILRAKCGPLTGMRESSIITNGTSSGFNSSVEDEDVKVKQESSDELKKDDFMKDVNLDSGDSLAEILMSRMLLFQKLTKQLSKKYNYAIRMNKSTGFFVSLLDTPSKKSDSKSGGSSFMLGNWKHPKVSNMSGFLAGDKDQLQKCPVYQDALGFVSPTGANEGSAPMQGMSLQGSTARMGGTQLPPIRSYKPITYTSSNLRRMNETGEAEAKRRRFNDGYIDNNSNNDIPRGISPKPSNGLSSVQPLLSSFSMNQLNGGTIPTVPSLTNITSQMGALPSLDRITTNQINLPDPSRDEAFDNSIKQMTPMTSAFMNANTTIPSSTLNGNMNMNGAGTANTDTSANGSALSTLTSPQGSDLASNSATQYKPDLEDFLMQNSNFNGLMINPSSLVEVVGGYNDPNNLGRNDAVDFLPVDNVEIDGVGIKINYHLLTSIYVTSILSYTVLEDDANDEK

Target ORF information:

RefSeq Version	NM_001182143.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C Hap1p (HAP1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182143.1

ORF Insert Sequence:

ATGTCGAATA CCCCTTATAA TTCATCTGTG CCTTCCATTG CATCCATGAC CCAGTCTTCG 
GTCTCAAGAA GTCCTAACAT GCATACAGCA ACTACGCCCG GTGCCAACAC CAGCTCTAAC 
TCTCCACCCT TGCACATGTC TTCAGATTCG TCCAAGATCA AGAGGAAGCG TAACAGAATT 
CCGCTCAGTT GCACCATTTG TCGGAAAAGG AAAGTCAAAT GTGACAAACT CAGACCACAC 
TGCCAGCAGT GCACTAAAAC TGGGGTAGCC CATCTCTGCC ACTACATGGA ACAGACCTGG 
GCAGAAGAGG CAGAGAAAGA ATTGCTGAAG GACAACGAAT TAAAGAAGCT TAGGGAGCGC 
GTAAAATCTT TAGAAAAGAC TCTTTCTAAG GTGCACTCTT CTCCTTCGTC TAACTCCTTG 
AAAAGTTACA ACACTCCCGA GAGCAGCAAC CTGTTTATGG GTAGCGATGA ACACACCACC 
CTTGTTAATG CAAATACAGG CTCTGCTTCC TCTGCCTCGC ATATGCATCA GCAACAACAG 
CAACAGCAGC AACAGGAACA ACAACAAGAC TTTTCCAGAA GTGCGAACGC CAACGCGAAT 
TCCTCGTCCC TTTCTATCTC AAATAAATAT GACAACGATG AGCTGGACTT AACTAAGGAC 
TTTGATCTTT TGCATATCAA AAGTAACGGA ACCATCCACT TAGGTGCCAC CCACTGGTTG 
TCTATCATGA AAGGTGACCC GTACCTAAAA CTTTTGTGGG GTCATATCTT CGCTATGAGG 
GAAAAGTTAA ATGAATGGTA CTACCAAAAA AATTCGTACT CTAAGCTGAA GTCAAGCAAA 
TGTCCCATCA ATCACGCGCA AGCGCCGCCT TCTGCCGCTG CCGCCGCTAC CAGAAAATGT 
CCTGTTGATC ACTCCGCGTT TTCGTCTGGC ATGGTGGCCC CAAAGGAGGA GACTCCTCTT 
CCTAGGAAAT GTCCAGTTGA CCACACCATG TTCTCTTCGG GAATGATTCC TCCCAGAGAG 
GACACTTCGT CCCAGAAGAG GTGTCCCGTT GACCACACCA TGTATTCCGC AGGAATGATG 
CCGCCCAAGG ACGAGACACC TTCCCCATTT TCCACTAAAG CTATGATAGA CCATAACAAG 
CATACAATGA ATCCGCCTCA GTCAAAATGT CCTGTGGACC ATAGAAACTA TATGAAGGAT 
TATCCCTCTG ACATGGCAAA TTCTTCTTCG AACCCGGCAA GTCGTTGCCC CATTGACCAT 
TCAAGCATGA AAAATACAGC GGCCTTACCA GCTTCAACGC ACAATACCAT CCCACACCAC 
CAACCACAGT CCGGATCTCA TGCTCGTTCG CATCCCGCAC AAAGCAGGAA ACATGATTCC 
TACATGACAG AATCTGAAGT CCTCGCAACA CTTTGTGAGA TGTTGCCACC AAAGCGCGTC 
ATCGCATTAT TCATCGAGAA ATTCTTCAAA CATTTATACC CTGCCATTCC AATCTTAGAT 
GAACAGAATT TCAAAAATCA CGTGAATCAA ATGCTTTCGT TGTCTTCGAT GAATCCCACA 
GTTAACAACT TTGGTATGAG CATGCCATCT TCATCTACAC TAGAGAACCA ACCCATAACA 
CAAATCAATC TTCCAAAACT TTCCGATTCT TGTAACTTAG GTATTCTGAT AATAATCTTG 
AGATTGACAT GGCTATCCAT ACCTTCTAAT TCCTGCGAAG TCGACCTGGG AGAAGAAAGT 
GGCTCATTTT TAGTGCCCAA CGAATCTAGC AATATGTCTG CATCTGCATT GACCTCGATG 
GCTAAAGAAG AATCACTTCT GCTAAAGCAT GAGACACCGG TCGAGGCACT GGAGCTATGT 
CAAAAATACT TGATTAAATT CGATGAACTT TCTAGTATTT CCAATAACAA CGTTAATTTA 
ACCACGGTGC AGTTTGCCAT TTTTTACAAC TTCTATATGA AAAGTGCCTC TAATGATTTG 
ACTACCTTGA CAAATACCAA CAACACTGGC ATGGCCAATC CTGGTCACGA TTCCGAGTCT 
CACCAGATCC TATTGTCCAA TATTACTCAA ATGGCCTTTA GTTGTGGGTT ACACAGAGAC 
CCTGATAATT TTCCTCAATT AAACGCTACC ATTCCAGCAA CCAGCCAGGA CGTGTCTAAC 
AACGGGAGCA AAAAGGCAAA CCCTAGCACC AATCCAACTT TGAATAACAA CATGTCTGCT 
GCCACTACCA ACAGCAGTAG CAGATCTGGC AGTGCTGATT CAAGAAGTGG TTCTAACCCT 
GTGAACAAGA AGGAAAATCA GGTTAGTATC GAAAGATTTA AACACACTTG GAGGAAAATT 
TGGTATTACA TTGTTAGCAT GGATGTTAAC CAATCTCTTT CCCTGGGGAG CCCTCGACTA 
CTAAGAAATC TGAGGGATTT CAGCGATACA AAGCTACCAA GTGCGTCAAG GATTGATTAT 
GTTCGCGATA TCAAAGAGTT AATCATTGTG AAGAATTTTA CTCTTTTTTT CCAAATTGAT 
TTGTGTATTA TTGCTGTATT AAATCACATT TTGAATGTTT CTTTAGCAAG AAGCGTGAGA 
AAATTTGAAC TGGATTCATT GATTAATTTA TTGAAAAATC TGACCTATGG TACTGAGAAT 
GTCAATGATG TAGTGAGCTC CTTGATCAAC AAAGGGTTAT TACCAACTTC GGAAGGTGGT 
TCTGTAGATT CAAATAATGA TGAAATTTAC GGTCTACCGA AACTACCCGA TATTCTAAAC 
CATGGTCAAC ATAACCAAAA CTTGTATGCT GATGGAAGAA ATACTTCTAG TAGTGATATA 
GATAAGAAAT TGGACCTTCC TCACGAATCT ACAACGAGAG CTCTATTCTT TTCCAAGCAT 
ATGACAATTA GAATGTTGCT GTACTTATTG AACTACATTT TGTTTACTCA TTATGAACCA 
ATGGGCAGTG AAGATCCTGG TACTAATATC CTAGCTAAGG AGTACGCTCA AGAGGCATTA 
AATTTTGCCA TGGATGGCTA CAGAAACTGC ATGATTTTCT TCAACAATAT CAGAAACACC 
AATTCACTAT TCGATTACAT GAATGTTATC TTGTCTTACC CTTGTTTGGA CATTGGACAT 
CGTTCTTTAC AATTTATCGT TTGTTTGATC CTGAGAGCTA AATGTGGCCC ATTGACTGGT 
ATGCGTGAAT CATCGATCAT TACTAATGGT ACATCAAGTG GATTTAATAG TTCGGTAGAA 
GATGAGGACG TCAAAGTTAA ACAAGAATCT TCTGATGAAT TGAAAAAAGA CGATTTCATG 
AAAGATGTAA ATTTGGATTC AGGCGATTCA TTAGCAGAGA TTCTAATGTC AAGAATGCTG 
CTATTTCAAA AACTAACAAA ACAACTATCA AAGAAGTACA ACTACGCTAT TCGTATGAAC 
AAATCCACTG GATTCTTTGT CTCTTTACTA GATACACCTT CAAAGAAATC AGACTCGAAA 
TCGGGTGGTA GTTCATTCAT GTTGGGTAAT TGGAAACATC CAAAGGTTTC AAACATGAGC 
GGATTTCTTG CTGGTGACAA AGACCAATTA CAGAAATGCC CCGTGTACCA AGATGCGCTG 
GGGTTTGTTA GTCCAACCGG TGCTAATGAA GGTTCTGCTC CGATGCAAGG CATGTCCTTA 
CAGGGCTCTA CTGCTAGGAT GGGAGGGACC CAGTTGCCAC CAATTAGATC ATACAAACCT 
ATCACGTACA CAAGTAGTAA TCTACGTCGT ATGAATGAAA CGGGTGAGGC AGAAGCTAAG 
AGAAGAAGAT TTAATGATGG CTATATTGAT AATAATAGTA ACAACGATAT ACCTAGAGGA 
ATCAGCCCAA AACCTTCAAA TGGGCTATCA TCGGTGCAGC CACTATTATC GTCATTTTCC 
ATGAACCAGC TAAACGGGGG TACCATTCCA ACGGTTCCAT CGTTAACCAA CATTACTTCA 
CAAATGGGAG CTTTACCATC TTTAGATAGG ATCACCACTA ATCAAATAAA TTTGCCAGAC 
CCATCTAGAG ATGAAGCATT TGACAACTCC ATCAAGCAAA TGACGCCTAT GACAAGTGCA 
TTCATGAATG CTAATACTAC AATTCCAAGT TCAACTTTAA ACGGGAATAT GAACATGAAT 
GGAGCTGGAA CTGCGAATAC AGATACAAGT GCCAACGGCA GTGCTTTATC GACACTGACA 
AGCCCACAAG GCTCAGACTT AGCATCCAAT TCTGCTACAC AGTATAAACC TGACTTAGAA 
GACTTTTTGA TGCAAAATTC TAACTTTAAT GGGCTAATGA TAAATCCTTC CAGTCTGGTA 
GAAGTTGTTG GTGGATACAA CGATCCTAAT AACCTTGGAA GAAATGACGC GGTTGATTTT 
CTACCCGTTG ATAATGTTGA AATTGATGGT GTTGGAATAA AAATCAACTA TCATCTACTA 
ACTAGTATTT ACGTTACTAG TATATTATCA TATACGGTGT TAGAAGATGA CGCAAATGAT 
GAGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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HAP1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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