YEF3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	YEF3
Entrez Gene ID	850951
Full Name	translation elongation factor EF-3
Synonyms	TEF3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001182136.1	NP_013350.1	translation elongation factor EF-3

Pathways from BioSystems

Homologies

Eremothecium gossypii	AGOS_AAL028W	NP_982514.2
Neurospora crassa	NCU07922	XP_962438.1
Schizosaccharomyces pombe (fission yeast)	tef3	NP_588285.1
Magnaporthe oryzae (rice blast fungus)	MGG_14971	XP_003720172.1
Kluyveromyces lactis	KLLA0F12210g	XP_455632.1
Saccharomyces cerevisiae (baker's yeast)	YEF3	NP_013350.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Johnston M, Hillier L, Riles L, Albermann K, Andr? B, Ansorge W, Benes V, Br?ckner M, Delius H, Dubois E, D?sterh?ft A, Entian KD, Floeth M, Goffeau A, Hebling U, Heumann K, Heuss-Neitzel D, Hilbert H, Hilger F, Kleine K, K?tter P, Louis EJ, Messenguy F, Mewes HW, Hoheisel JD
Nature387(6632 Suppl)87-90(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the elongation factor Yef3 was found to interact with mRNA in non-coding regions and in a translation independent manner. These results suggest an additional, non-elongation function for this factor.
Title: The elongation factor eEF3 (Yef3) interacts with mRNA in a translation independent manner.

Data indicate that the growth defect associated with constitutively active Gcn2 was diminished by eEF3 (YEF3) overexpression.
Title: Overexpression of eukaryotic translation elongation factor 3 impairs Gcn2 protein activation.

The following YEF3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the YEF3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00582	NM_001182136.1 Latest version!	Saccharomyces cerevisiae S288C translation elongation factor EF-3 (YEF3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

YEF3 ( NM_001182136.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013350.1 Saccharomyces cerevisiae S288C translation elongation factor EF-3 (YEF3), partial mRNA.

Price & Availability↑

CloneID	OSi00582
Clone ID Related Accession (Same CDS sequence)	NM_001182136.1
Accession Version	NM_001182136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3135bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	translation elongation factor EF-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001144). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTCTGATT CCCAGCAATC CATTAAGGTT CTAGAAGAAC TATTCCAGAA GTTATCTGTT 
GCCACTGCTG ACAACAGACA CGAAATCGCT TCTGAAGTCG CTTCTTTCTT GAATGGTAAC 
ATCATTGAAC ATGATGTTCC AGAACACTTC TTCGGTGAAT TGGCCAAGGG TATCAAGGAC 
AAGAAGACCG CTGCTAACGC CATGCAAGCT GTTGCTCACA TTGCTAACCA ATCTAACTTG 
TCTCCATCTG TTGAACCATA CATCGTCCAA TTGGTTCCAG CTATCTGTAC CAACGCAGGT 
AACAAGGACA AAGAAATTCA ATCTGTTGCT TCCGAAACTT TGATTTCCAT CGTTAACGCT 
GTTAACCCAG TTGCCATCAA AGCTTTGTTG CCACATTTGA CTAACGCTAT TGTCGAAACT 
AACAAATGGC AAGAAAAGAT TGCTATTTTG GCAGCTATCT CTGCTATGGT CGATGCTGCT 
AAGGATCAAG TTGCCCTAAG AATGCCAGAA TTGATTCCAG TCTTGTCTGA AACCATGTGG 
GACACCAAGA AGGAAGTCAA GGCTGCTGCT ACTGCCGCCA TGACCAAGGC TACCGAAACT 
GTTGACAACA AGGATATTGA ACGTTTCATT CCAAGTTTGA TTCAATGTAT TGCTGACCCA 
ACTGAAGTTC CAGAAACCGT TCATTTGTTA GGTGCTACTA CTTTCGTTGC TGAAGTTACT 
CCAGCTACTT TGTCCATCAT GGTCCCATTG TTGTCCAGAG GTTTGAACGA AAGAGAAACC 
GGTATCAAGC GTAAGTCTGC TGTTATTATT GACAACATGT GTAAGTTGGT CGAAGACCCA 
CAAGTTATTG CTCCTTTCTT GGGTAAATTG TTGCCAGGTT TGAAGAGTAA CTTTGCTACC 
ATTGCTGACC CAGAAGCCAG AGAAGTTACT TTGAGAGCTT TGAAGACTTT GAGAAGAGTT 
GGTAACGTTG GTGAAGACGA TGCTATTCCA GAAGTTTCTC ACGCTGGTGA CGTTTCTACT 
ACTTTGCAAG TCGTTAACGA ATTGTTGAAG GACGAAACCG TTGCTCCAAG ATTTAAGATT 
GTCGTCGAGT ACATTGCCGC CATTGGTGCT GATTTGATCG ATGAAAGAAT CATTGACCAA 
CAAGCTTGGT TCACCCACAT CACCCCATAC ATGACTATCT TCTTGCACGA AAAGAAGGCC 
AAGGACATCT TGGACGAATT CAGAAAGAGA GCTGTCGACA ACATTCCAGT TGGTCCAAAC 
TTCGACGACG AAGAAGACGA AGGTGAAGAC TTATGTAACT GTGAATTCTC TTTGGCTTAT 
GGTGCTAAAA TCTTGTTGAA CAAGACCCAA TTAAGATTGA AGAGAGCCAG AAGATATGGT 
ATCTGTGGTC CAAACGGTTG TGGTAAGTCC ACTTTAATGA GAGCTATTGC CAACGGTCAA 
GTTGATGGTT TCCCAACCCA AGAAGAATGT AGAACCGTCT ACGTCGAACA CGACATTGAT 
GGTACTCACT CTGACACTTC CGTCTTGGAT TTCGTTTTCG AATCTGGTGT TGGTACTAAA 
GAAGCTATCA AGGACAAATT GATTGAATTC GGTTTCACCG ATGAAATGAT TGCTATGCCA 
ATCTCTGCTT TGTCTGGTGG TTGGAAGATG AAGTTGGCTC TAGCTAGAGC TGTGTTGAGA 
AATGCTGATA TCTTGTTGTT AGATGAACCA ACTAACCATT TGGATACCGT CAACGTTGCT 
TGGTTAGTTA ACTACTTGAA CACCTGTGGT ATCACTTCTA TCACTATTTC TCACGACTCC 
GTTTTCTTAG ATAACGTCTG TGAATATATT ATTAACTACG AAGGTTTGAA GTTGAGAAAG 
TACAAGGGTA ACTTTACCGA ATTCGTTAAG AAGTGTCCAG CTGCTAAGGC TTACGAAGAA 
TTATCCAACA CTGATTTGGA ATTCAAGTTC CCAGAACCAG GTTACTTGGA AGGTGTTAAG 
ACTAAGCAAA AGGCTATTGT CAAGGTTACC AACATGGAAT TCCAATATCC AGGTACCTCT 
AAGCCACAAA TCACTGACAT TAACTTCCAA TGTTCTTTGT CTTCCAGAAT TGCTGTCATT 
GGTCCAAATG GTGCTGGTAA GTCTACTTTG ATTAACGTCT TGACTGGTGA ACTATTACCA 
ACCTCTGGTG AAGTCTACAC CCACGAAAAT TGTCGTATCG CTTACATTAA GCAACACGCT 
TTTGCTCATA TCGAATCTCA TTTGGACAAG ACTCCATCTG AATATATCCA ATGGAGATTC 
CAAACCGGTG AAGATAGAGA AACCATGGAC AGAGCTAACA GACAAATCAA CGAAAACGAT 
GCTGAAGCTA TGAACAAGAT CTTCAAGATT GAAGGTACCC CTAGAAGAAT TGCCGGTATC 
CACTCCAGAA GAAAGTTCAA GAACACTTAC GAATATGAAT GTTCTTTCTT ATTGGGTGAA 
AACATTGGTA TGAAATCTGA AAGATGGGTT CCAATGATGT CCGTCGACAA CGCTTGGATT 
CCAAGAGGTG AATTGGTTGA ATCTCACTCT AAGATGGTTG CTGAAGTTGA TATGAAGGAA 
GCTTTGGCTT CTGGTCAATT CCGTCCATTA ACCAGAAAAG AAATTGAAGA ACATTGTTCC 
ATGTTGGGTT TGGACCCAGA AATTGTTTCT CACTCCAGAA TTAGAGGTTT GTCTGGTGGT 
CAAAAGGTTA AGTTGGTCTT AGCTGCCGGT ACATGGCAAA GACCTCACTT GATTGTCTTA 
GATGAACCTA CCAACTATCT GGACAGAGAT TCTTTGGGTG CTTTGTCTAA GGCTTTGAAG 
GAATTTGAAG GTGGTGTTAT TATCATTACT CACTCTGCTG AATTCACAAA GAACTTGACT 
GAAGAAGTCT GGGCCGTCAA GGACGGTAGA ATGACTCCAT CTGGTCACAA CTGGGTTAGT 
GGTCAAGGTG CTGGTCCAAG AATCGAAAAG AAGGAAGACG AAGAAGATAA ATTCGATGCT 
ATGGGTAACA AGATTGCCGG TGGTAAGAAG AAGAAGAAGT TGTCTTCTGC GGAATTGAGA 
AAGAAGAAGA AGGAAAGAAT GAAGAAGAAG AAGGAATTGG GTGATGCTTA CGTTTCTTCT 
GACGAAGAAT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_013350.1
CDS	1..3135
Translation	MSDSQQSIKVLEELFQKLSVATADNRHEIASEVASFLNGNIIEHDVPEHFFGELAKGIKDKKTAANAMQAVAHIANQSNLSPSVEPYIVQLVPAICTNAGNKDKEIQSVASETLISIVNAVNPVAIKALLPHLTNAIVETNKWQEKIAILAAISAMVDAAKDQVALRMPELIPVLSETMWDTKKEVKAAATAAMTKATETVDNKDIERFIPSLIQCIADPTEVPETVHLLGATTFVAEVTPATLSIMVPLLSRGLNERETGIKRKSAVIIDNMCKLVEDPQVIAPFLGKLLPGLKSNFATIADPEAREVTLRALKTLRRVGNVGEDDAIPEVSHAGDVSTTLQVVNELLKDETVAPRFKIVVEYIAAIGADLIDERIIDQQAWFTHITPYMTIFLHEKKAKDILDEFRKRAVDNIPVGPNFDDEEDEGEDLCNCEFSLAYGAKILLNKTQLRLKRARRYGICGPNGCGKSTLMRAIANGQVDGFPTQEECRTVYVEHDIDGTHSDTSVLDFVFESGVGTKEAIKDKLIEFGFTDEMIAMPISALSGGWKMKLALARAVLRNADILLLDEPTNHLDTVNVAWLVNYLNTCGITSITISHDSVFLDNVCEYIINYEGLKLRKYKGNFTEFVKKCPAAKAYEELSNTDLEFKFPEPGYLEGVKTKQKAIVKVTNMEFQYPGTSKPQITDINFQCSLSSRIAVIGPNGAGKSTLINVLTGELLPTSGEVYTHENCRIAYIKQHAFAHIESHLDKTPSEYIQWRFQTGEDRETMDRANRQINENDAEAMNKIFKIEGTPRRIAGIHSRRKFKNTYEYECSFLLGENIGMKSERWVPMMSVDNAWIPRGELVESHSKMVAEVDMKEALASGQFRPLTRKEIEEHCSMLGLDPEIVSHSRIRGLSGGQKVKLVLAAGTWQRPHLIVLDEPTNYLDRDSLGALSKALKEFEGGVIIITHSAEFTKNLTEEVWAVKDGRMTPSGHNWVSGQGAGPRIEKKEDEEDKFDAMGNKIAGGKKKKKLSSAELRKKKKERMKKKKELGDAYVSSDEEF

Target ORF information:

RefSeq Version	NM_001182136.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C translation elongation factor EF-3 (YEF3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001182136.1

ORF Insert Sequence:

ATGTCTGATT CCCAGCAATC CATTAAGGTT CTAGAAGAAC TATTCCAGAA GTTATCTGTT 
GCCACTGCTG ACAACAGACA CGAAATCGCT TCTGAAGTCG CTTCTTTCTT GAATGGTAAC 
ATCATTGAAC ATGATGTTCC AGAACACTTC TTCGGTGAAT TGGCCAAGGG TATCAAGGAC 
AAGAAGACCG CTGCTAACGC CATGCAAGCT GTTGCTCACA TTGCTAACCA ATCTAACTTG 
TCTCCATCTG TTGAACCATA CATCGTCCAA TTGGTTCCAG CTATCTGTAC CAACGCAGGT 
AACAAGGACA AAGAAATTCA ATCTGTTGCT TCCGAAACTT TGATTTCCAT CGTTAACGCT 
GTTAACCCAG TTGCCATCAA AGCTTTGTTG CCACATTTGA CTAACGCTAT TGTCGAAACT 
AACAAATGGC AAGAAAAGAT TGCTATTTTG GCAGCTATCT CTGCTATGGT CGATGCTGCT 
AAGGATCAAG TTGCCCTAAG AATGCCAGAA TTGATTCCAG TCTTGTCTGA AACCATGTGG 
GACACCAAGA AGGAAGTCAA GGCTGCTGCT ACTGCCGCCA TGACCAAGGC TACCGAAACT 
GTTGACAACA AGGATATTGA ACGTTTCATT CCAAGTTTGA TTCAATGTAT TGCTGACCCA 
ACTGAAGTTC CAGAAACCGT TCATTTGTTA GGTGCTACTA CTTTCGTTGC TGAAGTTACT 
CCAGCTACTT TGTCCATCAT GGTCCCATTG TTGTCCAGAG GTTTGAACGA AAGAGAAACC 
GGTATCAAGC GTAAGTCTGC TGTTATTATT GACAACATGT GTAAGTTGGT CGAAGACCCA 
CAAGTTATTG CTCCTTTCTT GGGTAAATTG TTGCCAGGTT TGAAGAGTAA CTTTGCTACC 
ATTGCTGACC CAGAAGCCAG AGAAGTTACT TTGAGAGCTT TGAAGACTTT GAGAAGAGTT 
GGTAACGTTG GTGAAGACGA TGCTATTCCA GAAGTTTCTC ACGCTGGTGA CGTTTCTACT 
ACTTTGCAAG TCGTTAACGA ATTGTTGAAG GACGAAACCG TTGCTCCAAG ATTTAAGATT 
GTCGTCGAGT ACATTGCCGC CATTGGTGCT GATTTGATCG ATGAAAGAAT CATTGACCAA 
CAAGCTTGGT TCACCCACAT CACCCCATAC ATGACTATCT TCTTGCACGA AAAGAAGGCC 
AAGGACATCT TGGACGAATT CAGAAAGAGA GCTGTCGACA ACATTCCAGT TGGTCCAAAC 
TTCGACGACG AAGAAGACGA AGGTGAAGAC TTATGTAACT GTGAATTCTC TTTGGCTTAT 
GGTGCTAAAA TCTTGTTGAA CAAGACCCAA TTAAGATTGA AGAGAGCCAG AAGATATGGT 
ATCTGTGGTC CAAACGGTTG TGGTAAGTCC ACTTTAATGA GAGCTATTGC CAACGGTCAA 
GTTGATGGTT TCCCAACCCA AGAAGAATGT AGAACCGTCT ACGTCGAACA CGACATTGAT 
GGTACTCACT CTGACACTTC CGTCTTGGAT TTCGTTTTCG AATCTGGTGT TGGTACTAAA 
GAAGCTATCA AGGACAAATT GATTGAATTC GGTTTCACCG ATGAAATGAT TGCTATGCCA 
ATCTCTGCTT TGTCTGGTGG TTGGAAGATG AAGTTGGCTC TAGCTAGAGC TGTGTTGAGA 
AATGCTGATA TCTTGTTGTT AGATGAACCA ACTAACCATT TGGATACCGT CAACGTTGCT 
TGGTTAGTTA ACTACTTGAA CACCTGTGGT ATCACTTCTA TCACTATTTC TCACGACTCC 
GTTTTCTTAG ATAACGTCTG TGAATATATT ATTAACTACG AAGGTTTGAA GTTGAGAAAG 
TACAAGGGTA ACTTTACCGA ATTCGTTAAG AAGTGTCCAG CTGCTAAGGC TTACGAAGAA 
TTATCCAACA CTGATTTGGA ATTCAAGTTC CCAGAACCAG GTTACTTGGA AGGTGTTAAG 
ACTAAGCAAA AGGCTATTGT CAAGGTTACC AACATGGAAT TCCAATATCC AGGTACCTCT 
AAGCCACAAA TCACTGACAT TAACTTCCAA TGTTCTTTGT CTTCCAGAAT TGCTGTCATT 
GGTCCAAATG GTGCTGGTAA GTCTACTTTG ATTAACGTCT TGACTGGTGA ACTATTACCA 
ACCTCTGGTG AAGTCTACAC CCACGAAAAT TGTCGTATCG CTTACATTAA GCAACACGCT 
TTTGCTCATA TCGAATCTCA TTTGGACAAG ACTCCATCTG AATATATCCA ATGGAGATTC 
CAAACCGGTG AAGATAGAGA AACCATGGAC AGAGCTAACA GACAAATCAA CGAAAACGAT 
GCTGAAGCTA TGAACAAGAT CTTCAAGATT GAAGGTACCC CTAGAAGAAT TGCCGGTATC 
CACTCCAGAA GAAAGTTCAA GAACACTTAC GAATATGAAT GTTCTTTCTT ATTGGGTGAA 
AACATTGGTA TGAAATCTGA AAGATGGGTT CCAATGATGT CCGTCGACAA CGCTTGGATT 
CCAAGAGGTG AATTGGTTGA ATCTCACTCT AAGATGGTTG CTGAAGTTGA TATGAAGGAA 
GCTTTGGCTT CTGGTCAATT CCGTCCATTA ACCAGAAAAG AAATTGAAGA ACATTGTTCC 
ATGTTGGGTT TGGACCCAGA AATTGTTTCT CACTCCAGAA TTAGAGGTTT GTCTGGTGGT 
CAAAAGGTTA AGTTGGTCTT AGCTGCCGGT ACATGGCAAA GACCTCACTT GATTGTCTTA 
GATGAACCTA CCAACTATCT GGACAGAGAT TCTTTGGGTG CTTTGTCTAA GGCTTTGAAG 
GAATTTGAAG GTGGTGTTAT TATCATTACT CACTCTGCTG AATTCACAAA GAACTTGACT 
GAAGAAGTCT GGGCCGTCAA GGACGGTAGA ATGACTCCAT CTGGTCACAA CTGGGTTAGT 
GGTCAAGGTG CTGGTCCAAG AATCGAAAAG AAGGAAGACG AAGAAGATAA ATTCGATGCT 
ATGGGTAACA AGATTGCCGG TGGTAAGAAG AAGAAGAAGT TGTCTTCTGC GGAATTGAGA 
AAGAAGAAGA AGGAAAGAAT GAAGAAGAAG AAGGAATTGG GTGATGCTTA CGTTTCTTCT 
GACGAAGAAT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.
Nature387(6632 Suppl)87-90(1997 May)
Johnston M,Hillier L,Riles L,Albermann K,Andr? B,Ansorge W,Benes V,Br?ckner M,Delius H,Dubois E,D?sterh?ft A,Entian KD,Floeth M,Goffeau A,Hebling U,Heumann K,Heuss-Neitzel D,Hilbert H,Hilger F,Kleine K,K?tter P,Louis EJ,Messenguy F,Mewes HW,Hoheisel JD

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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YEF3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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